Moisés Castellá, Alberto Mestres-Arenas, Aleix Gavaldà-Navarro, Albert Blasco-Roset, Tania Quesada-López, Inés Romero-Carramiñana, Marta Giralt, Francesc Villarroya, Rubén Cereijo
The ability of alternative splicing mechanisms to control gene expression is increasingly being recognized as relevant for adipose tissue function. The expression of SF3B1, a key component of the SF3B complex directly involved in spliceosome formation, was previously reported to be significantly induced in brown adipose tissue under cold-induced thermogenic activation. Here, we identify that noradrenergic cAMP-mediated thermogenic stimulation increases SF3B1 expression in brown and beige adipocytes. We further show that pladienolide-B, a drug that binds SF3B1 to inhibit pre-mRNA splicing by targeting the SF3B complex, down-regulates key components of the thermogenic machinery (e...
December 28, 2023: Biochemical Pharmacology