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Dendritic cells, cigarette smoke

Sylvia M Kiertscher, Pallavi R Gangalum, Grace Ibrahim, Donald P Tashkin, Michael D Roth
Exposure to Δ9-tetrahydrocannabinol (THC) in vitro and in animal models can significantly impair the differentiation, activation and function of dendritic cells, T cells and B cells. However, studies directly assessing the impact of marijuana smoking on human immunity are lacking. A prospective study of immune responses to a standard hepatitis B vaccination was therefore carried out in a matched cohort of 9 marijuana smokers (MS) and 9 nonsmokers (NS). In addition to their regular marijuana use, MS smoked four marijuana cigarettes in a monitored setting on the day of each vaccination...
January 16, 2018: Journal of Neuroimmune Pharmacology: the Official Journal of the Society on NeuroImmune Pharmacology
Shi-Lin Qiu, Liang-Jian Kuang, Qi-Ya Tang, Min-Chao Duan, Jing Bai, Zhi-Yi He, Jian-Quan Zhang, Mei-Hua Li, Jing-Min Deng, Guang-Nan Liu, Xiao-Ning Zhong
Plasmacytoid dendritic cells (pDCs) are key cells bridging the innate with adaptive immunity. However, the phenotypic characteristics of circulating pDCs and its role in smoking related-Chronic Obstructive Pulmonary Disease (COPD) remain largely unknown. The aim of this study was analyzed the phenotype of circulating pDCs and the expression of IFN-γ producing CD8(+)T cells and IL-17-producing CD8(+)T cells in patients with COPD by using multi-colour flow cytometry. The cytokine profiles in peripheral blood from all subjects were measured by ELISA...
November 7, 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
Haihong Gong, Tao Liu, Wei Chen, Weixun Zhou, Jinming Gao
BACKGROUND: IRAK-M, negatively regulating Toll-like receptor, is shown the dual properties in the varied disease contexts. We studied the effect of IRAK-M deficiency on cigarette smoking- (CS-) induced airway inflammation under acute or subacute conditions in a mouse model. METHODS: A number of cellular and molecular techniques were used to detect the differences between IRAK-M knockout (KO) and wild type (WT) mice exposed to 3-day or 7-week CS. RESULTS: Airway inflammation was comparable between IRAK-M KO and WT mice under 3-day CS exposure...
2017: Mediators of Inflammation
Farrah Kheradmand, R You, Bon Hee Gu, D B Corry
Smoking-related lung diseases are among the most preventable and incurable ailments in the world. Smokers are at increased risk of developing chronic obstructive pulmonary disease that can be further complicated by emphysema and lung cancer. A subset of former smokers shows persistent lung inflammation and progressive loss of lung function, indicating a role for activation of acquired immunity in smoking-induced lung diseases. In addition to the well-established noxious effects of volatile compounds in cigarette smoke, incomplete combustion of tobacco generates nano-sized carbon black (nCB) that accumulate in lung myeloid dendritic cells and macrophages...
2017: Transactions of the American Clinical and Climatological Association
Shi-Lin Qiu, Hui Zhang, Qi-Ya Tang, Jing Bai, Zhi-Yi He, Jian-Quan Zhang, Mei-Hua Li, Jing-Min Deng, Guang-Nan Liu, Xiao-Ning Zhong
BACKGROUND: Neutrophil extracellular traps (NETs) represent a distinct strategy by which neutrophils trap, confine and eliminate invading microorganisms. Emerging evidence suggests that NETs exert a deleterious effect to the host in the absence of microbial stimuli. However, the role of NETs in smoking-related lung diseases remains to be elucidated. OBJECTIVES: To evaluate the formation of NETs in the context of chronic inflammation induced by cigarette smoking and explore its potential role in an experimental mouse model of emphysema...
July 18, 2017: Thorax
Thayse Regina Brüggemann, Paula Fernandes, Luana de Mendonça Oliveira, Maria Notomi Sato, Mílton de Arruda Martins, Fernanda Magalhães Arantes-Costa
Asthma is an allergic lung disease and, when associated to cigarette smoke exposition, some patients show controversial signs about lung function and other inflammatory mediators. Epidemiologic and experimental studies have shown both increasing and decreasing inflammation in lungs of subjects with asthma and exposed to cigarette smoke. Therefore, in this study, we analyzed how cigarette smoke affects pro-inflammatory and anti-inflammatory mediators in a murine model of allergic pulmonary inflammation. We sensitized Balb/c mice to ovalbumin (OVA) with two intraperitoneal injections...
2017: Frontiers in Immunology
Jennifer K Mulligan, Brendan P O'Connell, Whitney Pasquini, Ryan M Mulligan, Sarah Smith, Zachary M Soler, Carl Atkinson, Rodney J Schlosser
BACKGROUND: In these studies we examined the impact of environmental tobacco smoke (ETS) and active smoking on sinonasal dendritic cell (DC) subsets in controls or patients with chronic rhinosinusitis with nasal polyps (CRSwNP). In subsequent in-vitro investigations, we examined the influence of cigarette smoke extract (CSE) on human sinonasal epithelial cells' (HSNECs) ability to regulate DC functions. METHODS: Sinonasal tissue, blood, and hair were collected from patients undergoing sinus surgery...
August 2017: International Forum of Allergy & Rhinology
Gaetano Finocchiaro, Tiziana Langella, Cristina Corbetta, Serena Pellegatta
Checkpoint inhibitors, like ipilimumab, nivolumab, and pembrolizumab, have provided a breakthrough in cancer immunotherapy, such as in the treatment of melanoma and colorectal and lung cancer. The close relationship between the number of mutations (mutational load) and the response to checkpoint immunotherapy has been convincingly demonstrated in these cancers. Hypermutations in tumors are caused by environmental factors, like UV radiations or cigarette smoking, or by germinal mutations affecting genes of the Mismatch Repair (MMR) machinery, as in the Lynch syndrome...
February 2017: Discovery Medicine
Cecilie Ammitzbøll, Lars Börnsen, Jeppe Romme Christensen, Rikke Ratzer, Birgitte Romme Nielsen, Helle B Søndergaard, Marina R von Essen, Finn Sellebjerg
Upon chronic cigarette smoke exposure, inhaled antigens and irritants cause altered lung immune homeostasis. Circulating immune cells are affected, and smoking is associated with an increased risk of developing various disorders, including multiple sclerosis (MS). This study was conducted to determine the impact of smoking on circulating immune cell subsets. Furthermore, we determined whether any smoking-associated changes were related to MS. With the use of flow cytometry, CFSE assays, and ELISpot assays, we analyzed circulating immune cell phenotypes and quantified antigen-induced proliferation and cytokine secretion in smokers and nonsmokers in a cohort of 100 healthy individuals (HI)...
May 2017: Journal of Leukocyte Biology
Desheng Sun, Yao Ouyang, Yanhui Gu, Xiansheng Liu
AIM: To evaluate whether the effect of dendritic cells (DCs) on chronic obstructive pulmonary disease (COPD) can be relieved by blocking CCL20. METHODS: 30 Wistar rats were randomly divided into three groups: control, COPD, and COPD treated with CCL20 monoclonal antibody. In the latter two groups, COPD was induced by four-week cigarette smoke exposure and trachea injection of lipopolysaccharide solution on two occasions. CCL20 monoclonal antibody was injected intraperitoneally on the first day...
August 31, 2016: Croatian Medical Journal
Olivier Le Rouzic, Bachirou Koné, Jerome Kluza, Philippe Marchetti, Florence Hennegrave, Cécile Olivier, Gwenola Kervoaze, Eva Vilain, Clémence Mordacq, Nicolas Just, Thierry Perez, Nathalie Bautin, Muriel Pichavant, Philippe Gosset
BACKGROUND: Chronic obstructive pulmonary disease (COPD) is associated with chronic inflammation and impaired immune response to pathogens leading to bacteria-induced exacerbation of the disease. A defect in Th17 cytokines in response to Streptococcus pneumoniae, a bacteria associated with COPD exacerbations, has been recently reported. Dendritic cells (DC) are professional antigen presenting cells that drive T-cells differentiation and activation. In this study, we hypothesized that exposure to cigarette smoke, the main risk factor of COPD, might altered the pro-Th17 response to S...
2016: Respiratory Research
Liang-Jian Kuang, Ting-Ting Deng, Qin Wang, Shi-Lin Qiu, Yi Liang, Zhi-Yi He, Jian-Quan Zhang, Jing Bai, Mei-Hua Li, Jing-Min Deng, Guang-Nan Liu, Ji-Feng Liu, Xiao-Ning Zhong
Dendritic cells and CD8(+) T cells participate in the pathology of chronic obstructive pulmonary disease, including emphysema, but little is known of the involvement of the CD40/CD40L pathway. We investigated the role of the CD40/CD40L pathway in Tc1 cell differentiation induced by dendritic cells in a mouse model of emphysema, and in vitro. C57BL/6J wild-type and CD40(-/-) mice were exposed to cigarette smoke (CS) or not (control), for 24 wk. In vitro experiments involved wild-type and CD40(-/-) dendritic cells treated with CS extract (CSE) or not...
September 1, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
Elena Arellano-Orden, Carmen Calero-Acuña, Nicolás Moreno-Mata, Lourdes Gómez-Izquierdo, Verónica Sánchez-López, Cecilia López-Ramírez, Daniela Tobar, José Luis López-Villalobos, Cesar Gutiérrez, Ana Blanco-Orozco, José Luis López-Campos
BACKGROUND: Conflicting data exist on the role of pulmonary dendritic cells (DCs) and their maturation in patients with chronic obstructive pulmonary disease (COPD). Herein, we investigated whether disease severity and smoking status could affect the distribution and maturation of DCs in lung tissues of patients undergoing elective pneumectomy or lobectomy for suspected primary lung cancer. MATERIALS AND METHODS: A total of 75 consecutive patients were included...
2016: PloS One
Yi Liang, Guang Zhou, Longju Zhang, Jifeng Liu, Xiaoning Zhong
OBJECTIVE: To explore the impact of both cigarette smoke extracts (CSE) and CD40-CD40L pathway blocking on mouse myeloid dendritic cells (DCs) inducing CD4(+)T cells differentiation into CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs). METHODS :A combination of recombinant mouse granulocyte-macrophage colony stimulating factor (rmGM-CSF, 40 ng/mL) and recombinant mouse IL-4 (rmIL-4, 10 ng/mL) was applied in vitro to induce the differentiation of BALB/c mouse myeloid monocytes into DCs...
January 2016: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Masoumeh Ezzati Givi, Gert Folkerts, Gerry T M Wagenaar, Frank A Redegeld, Esmaeil Mortaz
BACKGROUND: Dendritic cells (DCs) as professional antigen presenting cells (APCs) play a critical role in the regulation of host immune responses. DCs evolve from immature, antigen-capturing cells, to mature antigen-presenting cells. The relative contribution of DCs to cigarette smoke-induced inflammation is not well documented. In the current study, we investigated a modulatory effect of cigarette smoke extract (CSE) on differentiation, maturation and function of DCs. METHODS: Primary murine DCs were grown from bone marrow cells with GM-CSF...
2015: Respiratory Research
Masoumeh Ezzati Givi, Peyman Akbari, Louis Boon, Vladimir S Puzovic, Gillina F G Bezemer, Fabio L M Ricciardolo, Gert Folkerts, Frank A Redegeld, Esmaeil Mortaz
The recruitment and activation of inflammatory cells into the respiratory system is considered a crucial feature in the pathophysiology of chronic obstructive pulmonary disease (COPD). Because dendritic cells (DCs) have a pivotal role in the onset and regulation of immune responses, we investigated the effect of modulating DC subsets on airway inflammation by acute cigarette smoke (CS) exposure. CS-exposed mice (5 days) were treated with fms-like tyrosine kinase 3 ligand (Flt3L) and 120g8 antibody to increase total DC numbers and deplete plasmacytoid DCs (pDCs), respectively...
January 1, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
Ran You, Wen Lu, Ming Shan, Jacob M Berlin, Errol Lg Samuel, Daniela C Marcano, Zhengzong Sun, William Ka Sikkema, Xiaoyi Yuan, Lizhen Song, Amanda Y Hendrix, James M Tour, David B Corry, Farrah Kheradmand
Chronic inhalation of cigarette smoke is the major cause of sterile inflammation and pulmonary emphysema. The effect of carbon black (CB), a universal constituent of smoke derived from the incomplete combustion of organic material, in smokers and non-smokers is less known. In this study, we show that insoluble nanoparticulate carbon black (nCB) accumulates in human myeloid dendritic cells (mDCs) from emphysematous lung and in CD11c(+) lung antigen presenting cells (APC) of mice exposed to smoke. Likewise, nCB intranasal administration induced emphysema in mouse lungs...
October 5, 2015: ELife
Leen J M Seys, Fien M Verhamme, Anja Schinwald, Hamida Hammad, Danen Mootoosamy Cunoosamy, Claudie Bantsimba-Malanda, Alan Sabirsh, Eileen McCall, Liz Flavell, Ronald Herbst, Sharen Provoost, Bart N Lambrecht, Guy F Joos, Guy G Brusselle, Ken R Bracke
RATIONALE: B cell-activating factor (BAFF) plays a major role in activation of B cells and in adaptive humoral immune responses. In chronic obstructive pulmonary disease (COPD), lymphoid follicles have been associated with disease severity, and overexpression of BAFF has been demonstrated within lymphoid follicles of patients with severe COPD. OBJECTIVES: To investigate expression and localization of BAFF in the lungs of patients with COPD and to study the role of BAFF in COPD by antagonizing BAFF in a mouse model of chronic cigarette smoke (CS) exposure...
September 15, 2015: American Journal of Respiratory and Critical Care Medicine
Robert Vassallo, Lei Wang, Yoshimi Hirano, Paula Walters, Diane Grill
The tobacco industry has promoted certain cigarette products with claims that their use may be less harmful to the smoker as they purportedly deliver lower amounts of toxic chemicals compared to conventional cigarettes. This study was designed to compare the relative antigen presenting cellular toxicity of Eclipse, a presumed reduced exposure product (PREP) cigarette, when compared with the reference research 3R4F cigarettes (Kentucky University). Utilizing a murine macrophage cell line, murine bone marrow derived dendritic cells (DCs) and human monocyte-derived DCs incubated with extracts generated from Eclipse and Kentucky reference 3R4F cigarettes, we determined the relative toxic effects of the different cigarette smoke extracts on cellular viability, oxidative stress, T-helper-1 (Th-1) polarizing cytokine production and general gene expression...
September 1, 2015: Toxicology
Mitsuo Hashimoto, Haruhiko Yanagisawa, Shunsuke Minagawa, Debasish Sen, Royce Ma, Lynne A Murray, Ping Tsui, Jianlong Lou, James D Marks, Jody L Baron, Matthew F Krummel, Stephen L Nishimura
Small airway chronic inflammation is a major pathologic feature of chronic obstructive pulmonary disease (COPD) and is refractory to current treatments. Dendritic cells (DCs) accumulate around small airways in COPD. DCs are critical mediators of Ag surveillance and Ag presentation and amplify adaptive immune responses. How DCs accumulate around airways remains largely unknown. We use 2-photon DC imaging of living murine lung sections to directly visualize the dynamic movement of living DCs around airways in response to either soluble mediators (IL-1β) or environmental stimuli (cigarette smoke or TLR3 ligands) implicated in COPD pathogenesis...
August 1, 2015: Journal of Immunology: Official Journal of the American Association of Immunologists
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