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William J Hickey, Ameesha R Shetty, Randall J Massey, Daniel B Toso, Jotham Austin
Bacterial biofilms play key roles in environmental and biomedical processes, and understanding their activities requires comprehension of their nanoarchitectural characteristics. Electron microscopy (EM) is an essential tool for nanostructural analysis, but conventional EM methods are limited in that they either provide topographical information alone, or are suitable for imaging only relatively thin (<300 nm) sample volumes. For biofilm investigations, these are significant restrictions. Understanding structural relations between cells requires imaging of a sample volume sufficiently large to encompass multiple cells and the capture of both external and internal details of cell structure...
August 12, 2016: Journal of Microscopy
H Goost, K Kabir, C Burger, P Pennekamp, H Röhrig, D C Wirtz, C Deborre, A Rabanus
BACKGROUND: Instability of the sternoclavicular joint is a very uncommon disorder of the shoulder girdle. Acute traumatic dislocations are commonly treated nonoperatively. But severe displacement or chronic instability with recurrent symptomatic subluxation may require surgical intervention. We present our results with open reduction and internal fixation through an autologous gracilis tendon transplant or fiber tape in 8 patients treated surgically. The operative stabilisation of the sternoclavicular joint reduces pain level and improves function of the shoulder...
August 2015: Operative Orthopädie und Traumatologie
M Gargari, B Lorè, F M Ceruso
OBJECTIVE: The aim of this study is to report a case of restorative treatment of severely worn dentition. In this case report, the erosive/abrasive worn dentition have been reconstructed with metal ceramic crown on the posterior teeth and with ceramic veneers on the anterior teeth 1.3 - 2.3 and 3.4 - 4.3. METHODS: A prosthetic treatment was proposed to a male patient of 58 years old having a clinically significant tooth wear. After clinical exam, impressions of maxillary and mandible arches were taken with alginate to obtain preliminary casts for diagnostic waxing to all maxillary and mandibular teeth and fabrication of all provisional crowns in acrylic resin for posterior teeth, and from the diagnostic wax-up were fabricated a silicone guide masks for anterior teeth...
April 2014: Oral & Implantology
Roberto Cuomo, Irene Zerini, Gaia Botteri, Laura Barberi, Giuseppe Nisi, Carlo D'Aniello
BACKGROUND: After a review of clinical cases of the Unit of Plastic Surgery of the University of Siena, Italy, we found that 22 patients undergoing lipofilling for breast recontruction needed less pain drugs compared to 18 patients which did not undergo lipofilling. In this work, the postoperative pain was analyzed in two groups of patients: a cohort treated with prosthesis and a cohort treated with prosthesis implant together with a lipofilling procedure. PATIENTS AND METHODS: During the immediate postoperative period, a visual analog scale for pain was submitted to every patient every eight hours until they were discharged, then every day for a week, every two days during the second week and once a week in the first three months...
September 2014: In Vivo
G Márquez, A Pinto, L Alamo, B Baumann, F Ye, H Winkler, K Taylor, R Padrón
Myosin interacting-heads (MIH) motifs are visualized in 3D-reconstructions of thick filaments from striated muscle. These reconstructions are calculated by averaging methods using images from electron micrographs of grids prepared using numerous filament preparations. Here we propose an alternative method to calculate the 3D-reconstruction of a single thick filament using only a tilt series images recorded by electron tomography. Relaxed thick filaments, prepared from tarantula leg muscle homogenates, were negatively stained...
May 2014: Journal of Structural Biology
Jun Li, Liang-Jun Zhong, Hetaer-Huojia Mu, Li-Kun Duo, Guang-Peng Yan
OBJECTIVE: To observe the cellular morphological and histological changes of the reconstructed tongue defect by rectus abdominis musculoperitoneal flap of dogs with or without nerve. METHODS: 12 Beagle dogs were randomly divided into two groups. Group A made rectus abdominis musculoperitoneal flap with the intercostal nerve while group B without the intercostal nerve. Nerve anastomosis was performed in Group A while not in Group B in the repairment. 12 weeks later, the length, width, surface area and cellular morphology and histological changes of the two transfer flaps were observed...
November 2013: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
R Dimitrov
Images of the levator hiatus in 40 nulliparous, 40 asymptomatic parous women and 40 women with pelvic organ prolapse were obtained by transperineal/translabial three-dimentional ultrasonography of the pelvic floor. Volumes were taken at rest and during squeezing. Recontructed images of the levator hiatus at the plane of minimal dimentions were obtained by off-line processing of the stored volumes. The transverse, the longitudinal diameter and the area of the levator hiatus were measured at rest and during squeezing in the three groups...
2013: Akusherstvo i Ginekologii︠a︡
Predrag Milojević, S Mićović, Djordje Radak, Petar Vuković, Bosko Djukanović
OBJECTIVES: There is still a dilemma about best treatment option for patients with severe carotid and coronary artery disease. Reviving of beating heart revascularization technique and using of carotid stenting makes things even more difficult. Discussion about this subject is getting more and more profound. This is analysis of our initial experience with simultaneous carotid and off pump coronary procedures. MATERIALS AND METHODS: This is retrospective analysis of 18 patients operated using this technique in a period from 2001-2003 yrs...
2012: Acta Chirurgica Iugoslavica
Lazar Davidović, Miodrag Jevtić, Djordje Radak, Dragan Sagić, Ivan Marjanović, Igor Koncar, Momcilo Colić, Sinisa Rusović, Zelimir Antonić
BACKGROUND/AIM: Endovascular treatment of thoracic aortic diseases is an adequate alternative to open surgery. This method was firstly performed in Serbia in 2004, while routine usage started in 2007. Aim of this study was to analyse initial experience in endovacular treatment of thoracic aortic diseses of three main vascular hospitals in Belgrade - Clinic for Vascular and Endovascular Surgery of the Clinical Center of Serbia, Clinic for Vascular Surgery of the Military Medical Academy, and Clinic for Vascular Surgery of the Institute for Cardiovascular Diseases "Dedinje"...
January 2013: Vojnosanitetski Pregled. Military-medical and Pharmaceutical Review
Zairong Wei, Guangfeng Sun, Xiujun Tang, Chengliang Deng, Wenhu Jin, Dali Wang, Bo Wang
OBJECTIVE: To investigate the characteristics of blood supply of popliteal fossa middle artery pedicled flaps and the feasibility of reconstruction of postburn popliteal fossa contractures using the flaps in children. METHODS: Between January 2008 and October 2010, 6 cases of postburn popliteal fossa contractures were recontructed using popliteal fossa middle artery pedicled flaps. Of them, 2 were boys and 4 were girls, aged from 2 years and 2 months to 10 years...
August 2012: Chinese Journal of Reparative and Reconstructive Surgery
Arjun Nair, Myrna C Godoy, Emma L Holden, Brendan P Madden, Felix Chua, David E Ost, Justus E Roos, David P Naidich, Ioannis Vlahos
A widening spectrum of increasingly advanced bronchoscopic techniques is available for the diagnosis and treatment of various bronchopulmonary diseases. The evolution of computed tomography (CT)-multidetector CT in particular-has paralleled these advances. The resulting development of two-dimensional and three-dimensional (3D) postprocessing techniques has complemented axial CT interpretation in providing more anatomically familiar information to the pulmonologist. Two-dimensional techniques such as multiplanar recontructions and 3D techniques such as virtual bronchoscopy can provide accurate guidance for increasing yield in transbronchial needle aspiration and transbronchial biopsy of mediastinal and hilar lymph nodes...
September 2012: Radiographics: a Review Publication of the Radiological Society of North America, Inc
Z Spurná, L Dražan, L Foretová, L Dvorská
UNLABELLED: It is presumed that bilateral mastectomy is so far the most efficient way how to prevent development of breast carcinoma among BRCA positive patients. This mutilating intervention might be unacceptable for cosmetic reasons for most women. The purpose of this study was to determine the influence of prophylactic mastectomy on the quality of life of BRCA positive patients by comparing results of psychodiagnostic questionnaire methods before surgical intervention and after it...
2012: Klinická Onkologie: Casopis Ceské a Slovenské Onkologické Spolecnosti
D Re, G Pellegrini, P Francinetti, D Augusti, G Rasperini
AIM: Zirconia is a ceramic material with optimal aesthetic and mechanical properties that make it adapt to esthetic recontructions in narrow spaces. Aim of this split mouth clinical trial is to compare amount and structure of early plaque coated to zirconia and feldspathic ceramic. METHODS: Four patients were included in this study. A removable acrylic device was manufactured for each subject and equipped on the lingual surface in correspondence to molar teeth with eight specimens...
July 2011: Minerva Stomatologica
Manlio Schettini
INTRODUCTION: Radical cystectomy is the most effective treatment madality for high grade urinary bladder carcinoma and orthotopic reconstruction is the better urinary diversion modality also in women. MATERIAL AND METHODS: From 2002 to 2007 we performed 14 radical cystectomies followed by orthotopic reconstruction in women aged between 47 and 68 years (mean age 56) affected by urinary bladder carcinoma. Our reconstructive technique requires the preparation of two strips of the recti muscles fascia, the sectioning of the bladder neck and, when the uterus is present, hysteroannessiectomy and cystectomy en block leaving intact the lateral and inferior vaginal walls...
December 2010: Archivio Italiano di Urologia, Andrologia
L Liu, Q Zhou, J Yang, Y Zhao, Y Wang, Z Wu, Y Shi
BACKGROUND: To summarize the experience of carinal resection and reconstruction about the surgical technique and perioperative management in the treatment of carinal and stem bronchial tumor and central bronchogenic carcinoma. METHODS: Eight cases of carinal and stem bronchial tumor and 27 cases of central bronchogenic carcinoma were included in this group, in which the left atrium was invaded in 5 cases. Types of surgery included carinal resection and reconstruction without pulmonary resection in 5 cases, carinal resection and reconstruction plus right upper lobectomy 18 cases, right carinal pneumonectomy 4 cases, left carinal pneumonectomy 4 cases, and carinal resection and recontruction plus left upper lobectomy 4 cases...
June 20, 2001: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
Yonglan Yu, Haili Zhang, Guan Zhu
BACKGROUND: The trehalose synthetic pathway is present in bacteria, fungi, plants and invertebrate animals, but is absent in vertebrates. This disaccharide mainly functions as a stress protectant against desiccation, heat, cold and oxidation. Genes involved in trehalose synthesis have been observed in apicomplexan parasites, but little was known about these enzymes. Study on trehalose synthesis in apicomplexans would not only shed new light into the evolution of this pathway, but also provide data for exploring this pathway as novel drug target...
2010: PloS One
S Dommerich, H-W Pau, T Lindner, T Just, J Ostwald
BACKGROUND: In cholesteatoma surgery the use of autogenous ossicles for restoration of sound conduction is often limited because of ingrowth of matrix epithelia into the ossicular bone. In an attempt to eliminated these epithelial cells we tested extracorporal high-pressure hydrostatic treatment as a new method for devitalizing the bone but maintaining its structure. METHODS: The inpact of different high hydrostatic pressures either on single cell suspensions of fibroblasts and osteoblasts, or on cells in ex-vivo ossicles chain was examined with different methods (quantitative proceedings, live/dead assay)...
May 2010: Laryngo- Rhino- Otologie
Gheorghe Burnei, Cristian Burnei, Dan Hodorogea, Stefan Gavriliu, Ileana Georgescu, Costel Vlad
This paper is a retrospective study on 8 patients admitted and treated in Paediatric Surgery and Orthopaedics Clinic of "M. S. Curie" Hospital Bucharest between 1997 and 2007. The patients with malignant bone tumors (table 1.) were studied by sex, tumor type, location, age at the moment of diagnosis, age at the moment of the last evaluation, type of surgery, external fixator implanted, complications, results and survival period. We also considered for each patient the extent of the tumor to diaphysis, soft tissue involvement, involvement of physis and epiphyseal invasion, articular extent, vessels and nerves invasion, presence of metastases and local skin invasion...
July 2008: Journal of Medicine and Life
Milan J A van Hoek, Paulien Hogeweg
Whole genome duplications (WGDs) have been hypothesized to be responsible for major transitions in evolution. However, the effects of WGD and subsequent gene loss on cellular behavior and metabolism are still poorly understood. Here we develop a genome scale evolutionary model to study the dynamics of gene loss and metabolic adaptation after WGD. Using the metabolic network of Saccharomyces cerevisiae as an example, we primarily study the outcome of WGD on yeast as it currently is. However, similar results were obtained using a recontructed hypothetical metabolic network of the pre-WGD ancestor...
November 2009: Molecular Biology and Evolution
Shan Lin, Ke-Jian Lian, Chang-Qing Chen, Wen-Liang Zhai, Lin-Xin Guo, Tao-Yi Cali
OBJECTIVE: To investigate the effect of treatment methods of non-operation and surgical operation for scapular fracture. METHODS: A retrospective analysis of 52 patients (male 37, female 15, ranging in age from 20 to 48 years, meanly 31 years)with scapular fractures was done. According to Hardegger classification: the scapular body fracture in 24 cases, the surgical neck fracture in 12 cases, the anatomical neck fracture in 3 cases, the glenoidal lip fracture in 6 cases, the scapular spine fracrure in 7 cases...
February 2008: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
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