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Ku antibody

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https://www.readbyqxmd.com/read/27903863/internalization-of-secreted-antigen-targeted-antibodies-by-the-neonatal-fc-receptor-for-precision-imaging-of-the-androgen-receptor-axis
#1
Daniel L J Thorek, Philip A Watson, Sang-Gyu Lee, Anson T Ku, Stylianos Bournazos, Katharina Braun, Kwanghee Kim, Kjell Sjöström, Michael G Doran, Urpo Lamminmäki, Elmer Santos, Darren Veach, Mesruh Turkekul, Emily Casey, Jason S Lewis, Diane S Abou, Marise R H van Voss, Peter T Scardino, Sven-Erik Strand, Mary L Alpaugh, Howard I Scher, Hans Lilja, Steven M Larson, David Ulmert
Targeting the androgen receptor (AR) pathway prolongs survival in patients with prostate cancer, but resistance rapidly develops. Understanding this resistance is confounded by a lack of noninvasive means to assess AR activity in vivo. We report intracellular accumulation of a secreted antigen-targeted antibody (SATA) that can be used to characterize disease, guide therapy, and monitor response. AR-regulated human kallikrein-related peptidase 2 (free hK2) is a prostate tissue-specific antigen produced in prostate cancer and androgen-stimulated breast cancer cells...
November 30, 2016: Science Translational Medicine
https://www.readbyqxmd.com/read/27865104/detection-of-low-abundance-biomarker-lipocalin-1-for-diabetic-retinopathy-using-optoelectrokinetic-bead-based-immunosensing
#2
Jhih-Cheng Wang, Hu-Yao Ku, Tain-Song Chen, Han-Sheng Chuang
Early diagnosis of diabetic retinopathy (DR) is vital but challenging. DR is a common complication and a major cause of vision loss in patients with diabetes mellitus. Without appropriate medical intervention, visual impairment may become a great burden to our healthcare system. In clinical practice, the current diagnostic methods, such as fluorescence angiography and optical coherence tomography, remain constrained by non-quantitative examinations and individual ophthalmologists' experiences. Late diagnosis often prevents early treatment...
November 10, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27835848/biochemical-characterization-and-immunolocalization-studies-of-a-capsicum-chinense-jacq-protein-fraction-containing-ding-proteins-and-anti-microbial-activity
#3
Ligia Brito-Argáez, José A Tamayo-Sansores, Dianeli Madera-Piña, Francisco J García-Villalobos, Rosa E Moo-Puc, Ángela Kú-González, Marco A Villanueva, Ignacio Islas-Flores
The DING protein family consists of proteins of great biological importance due to their ability to inhibit carcinogenic cell growth. A DING peptide with Mr ∼7.57 kDa and pI ∼5.06 was detected in G10P1.7.57, a protein fraction from Capsicum chinense Jacq. seeds. Amino acid sequencing of the peptide produced three smaller peptides showing identity to the DING protein family. G10P1.7.57 displayed a phosphatase activity capable of dephosphorylating different phosphorylated substrates and inhibited the growth of Saccharomyces cerevisiae cells...
November 3, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27793685/transcutaneous-immunization-via-rapidly-dissolvable-microneedles-protects-against-hand-foot-and-mouth-disease-caused-by-enterovirus-71
#4
Zhuangzhi Zhu, Xiaohua Ye, Zhiqiang Ku, Qingwei Liu, Chaoyun Shen, Huafei Luo, Hansen Luan, Chenghao Zhang, Shaoqiong Tian, CheeYen Lim, Zhong Huang, Hao Wang
Recent large outbreaks of hand-foot-and-mouth disease (HFMD) have seriously affected the health of young children. Enterovirus 71 (EV71) is the main causative agent of HFMD. Herein, for the first time, rapidly dissolvable microneedles (MNs) loaded with EV71 virus-like particles (VLPs) were evaluated whether they could induce robust immune responses that confer protection against EV71 infection. The characteristics of prepared MNs including hygroscopy, mechanical strength, insertion capacity, dissolution profile, skin irritation and storage stability were comprehensively assessed...
October 25, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/27684828/pulmonary-arterial-hypertension-in-idiopathic-inflammatory-myopathies-data-from-the-french-pulmonary-hypertension-registry-and-review-of-the-literature
#5
Sébastien Sanges, Cécile M Yelnik, Olivier Sitbon, Olivier Benveniste, Kuberaka Mariampillai, Mathilde Phillips-Houlbracq, Christophe Pison, Christophe Deligny, Jocelyn Inamo, Vincent Cottin, Luc Mouthon, David Launay, Marc Lambert, Pierre-Yves Hatron, Laurence Rottat, Marc Humbert, Eric Hachulla
Occurrence of pulmonary arterial hypertension (PAH) in idiopathic inflammatory myopathies (IIMs) without extensive interstitial lung disease (ILD) has rarely been described in the medical literature. This study aimed to report all cases with association of PAH and IIM in the French Pulmonary Hypertension (PH) Registry, to identify IIM features associated with the presence of PAH, and to describe treatment modalities of these patients.All cases of IIM-PAH were retrieved from the French PH Registry, which gathers PH patients prospectively enrolled by 27 referral hospital centers across France...
September 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27632981/interaction-of-interferon-alpha-therapy-with-thyroid-function-tests-in-the-management-of-hepatitis-c-a-case-report
#6
Gurmit Gill, Hammad Bajwa, Peter Strouhal, Harit N Buch
BACKGROUND: Interferon alpha is a widely used therapeutic agent in the treatment of hepatitis C virus infection. Clinical thyroid disease is seen in nearly 15 % of patients receiving interferon alpha for hepatitis C virus infection. The mechanism of thyroid dysfunction with interferon alpha is either autoimmune or inflammatory. We report a case of young woman who developed biphasic thyroid dysfunction posing a diagnostic challenge, while receiving interferon alpha treatment for hepatitis C virus infection...
2016: Journal of Medical Case Reports
https://www.readbyqxmd.com/read/27600137/comparisons-of-serum-infliximab-and-antibodies-to-infliximab-tests-used-in-inflammatory-bowel-disease-clinical-trials-of-remicade%C3%A2
#7
Joseph C Marini, Jocelyn Sendecki, Freddy Cornillie, John W Popp, Shawn Black, Marion Blank, Ann Gils, Thomas Van Stappen, Dörte Hamann, Theo Rispens, Lina Thérien, Kelly Chun, Gopi Shankar
Monitoring infliximab (IFX) concentrations and antibodies-to-IFX (ATI) titers during inflammatory bowel disease treatment may allow more informed decisions in assessing exposure/response and determining appropriate dosing. To aid in interpreting results from different commercial tests in the context of Janssen's published Remicade® results, the reliability of Janssen's IFX and ATI assays was compared with commercial assays from KU Leuven, Sanquin, Dynacare, and LabCorp. Test results were independently reported to Janssen...
September 6, 2016: AAPS Journal
https://www.readbyqxmd.com/read/27583908/single-specificity-anti-ku-antibodies-in-an-international-cohort-of-2140-systemic-sclerosis-subjects-clinical-associations
#8
S Hoa, M Hudson, Y Troyanov, S Proudman, J Walker, W Stevens, M Nikpour, S Assassi, M D Mayes, M Wang, M Baron, M J Fritzler
Autoantibodies directed against the Ku autoantigen are present in systemic sclerosis (SSc) and have been associated with myositis overlap and interstitial lung disease (ILD). However, there is a paucity of data on the clinical correlates of anti-Ku antibodies in the absence of other SSc-specific antibodies. The aim of this study was to assess the clinical correlates of single-specificity anti-Ku in SSc.An international (Canada, Australia, USA, Mexico) cohort of 2140 SSc subjects was formed, demographic and clinical variables were harmonized, and sera were tested for anti-Ku using a line immunoassay...
August 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27568879/tumour-cell-surface-antigen-targeted-therapies-in-b-cell-lymphomas-beyond-rituximab
#9
Matthew Ku, Geoff Chong, Eliza A Hawkes
Recent proliferation of novel targeted therapies in lymphoma has been substantial. B-cell receptor pathway inhibitors and immune checkpoint inhibitors have been a major focus, however significant advances in monoclonal antibodies (MoAbs) which directly target malignant cells have also occurred. These MoAbs continue to make significant impact in lymphoma management. Novel dosing schedules of anti-CD20 MoAb rituximab potentially optimise efficacy in specific lymphoma subgroups, as certain populations may be receiving suboptimal doses using current schedules...
August 12, 2016: Blood Reviews
https://www.readbyqxmd.com/read/27562093/inactivated-coxsackievirus-a10-experimental-vaccines-protect-mice-against-lethal-viral-challenge
#10
Chaoyun Shen, Qingwei Liu, Yu Zhou, Zhiqiang Ku, Lili Wang, Ke Lan, Xiaohua Ye, Zhong Huang
Coxsackievirus A10 (CVA10) has become one of the major causative agents of hand, foot and mouth disease (HFMD). It is now recognized that CVA10 should be targeted for vaccine development. We report here that β-propiolactone inactivated whole-virus based CVA10 vaccines can elicit protective immunity in mice. We prepared two inactivated CVA10 experimental vaccines derived from the prototype strain CVA10/Kowalik and from a clinical isolate CVA10/S0148b, respectively. Immunization with the experimental vaccines elicited CVA10-specific serum antibodies in mice...
September 22, 2016: Vaccine
https://www.readbyqxmd.com/read/27540627/pegylated-artificial-antibodies-plasmonic-biosensors-with-improved-selectivity
#11
Jingyi Luan, Keng-Ku Liu, Sirimuvva Tadepalli, Qisheng Jiang, Jeremiah J Morrissey, Evan D Kharasch, Srikanth Singamaneni
Molecular imprinting, which involves the formation of artificial recognition elements or cavities with complementary shape and chemical functionality to the target species, is a powerful method to overcome a number of limitations associated with natural antibodies. An important but often overlooked consideration in the design of artificial biorecognition elements based on molecular imprinting is the nonspecific binding of interfering species to noncavity regions of the imprinted polymer. Here, we demonstrate a universal method, namely, PEGylation of the noncavity regions of the imprinted polymer, to minimize the nonspecific binding and significantly enhance the selectivity of the molecular imprinted polymer for the target biomolecules...
September 14, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27538058/advances-in-serological-diagnostics-of-inflammatory-myopathies
#12
Olivier Benveniste, Werner Stenzel, Yves Allenbach
PURPOSE OF REVIEW: Inflammatory myopathies are rare diseases. Their diagnosis criteria are historically based on their clinical phenotype (topography of the muscle weakness, presence of skin lesions and/or of extra-skin/muscle signs) and the presence of inflammatory infiltrates on muscle biopsy. However, the recent discovery of different myositis-specific antibodies (MSA) or myositis-associated antibodies (MAA) permitted to revisit these old classifications. This review covers recent findings in clinical and pathological phenotypes regarding prognosis, associated cancer and response to the treatment based on MSA/MAA categorization...
October 2016: Current Opinion in Neurology
https://www.readbyqxmd.com/read/27525523/identification-of-a-major-epitope-by-anti-interferon-%C3%AE-autoantibodies-in-patients-with-mycobacterial-disease
#13
Chia-Hao Lin, Chih-Yu Chi, Han-Po Shih, Jing-Ya Ding, Chia-Chi Lo, Shang-Yu Wang, Chen-Yen Kuo, Chun-Fu Yeh, Kun-Hua Tu, Shou-Hsuan Liu, Hung-Kai Chen, Chen-Hsuan Ho, Mao-Wang Ho, Chen-Hsiang Lee, Hsin-Chin Lai, Cheng-Lung Ku
The binding of autoantibodies (autoAbs) to interferon (IFN)-γ in people with mycobacterial diseases has become an emerging medical concern. Many patients display specific human leukocyte antigen (HLA) class II haplotypes, which suggests that a common T cell-dependent and B cell-dependent mechanism might underlie the production of specific anti-IFN-γ autoAbs. We show here that these autoAbs target a major epitope (amino acids 121-131, designated position (P)121-131) in a region crucial for IFN-γ receptor (IFN-γR) activation to impair IFN-γ-mediated activities...
September 2016: Nature Medicine
https://www.readbyqxmd.com/read/27505432/real-life-study-for-the-diagnosis-of-house-dust-mite-allergy-the-value-of-recombinant-allergen-based-ige-serology
#14
Sven Becker, Thomas Schlederer, Matthias F Kramer, Mareike Haack, Susanne Vrtala, Yvonne Resch, Christian Lupinek, Rudolf Valenta, Moritz Gröger
BACKGROUND: Dermatophagoides pteronyssinus is one of the most important perennial allergen sources worldwide. Molecular diagnostics using the commercially available major allergens (Der p 1 and Der p 2) in combination with Der p 10 do not detect house dust mite (HDM) sensitization in a number of cases when used alone. The objective was to evaluate the IgE reactivity profiles of these patients using an experimental immunoassay biochip. METHODS: Sera of HDM-allergic patients (positive skin prick test, CAP class ≥1 for allergen extract, and positive intranasal provocation) were tested for IgE antibodies against Der p 1, Der p 2, and Der p 10 by ImmunoCAP fluorescence enzyme immunoassay...
2016: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/27477413/sarcoid-myositis-with-anti-ku-antibody-consistent-with-both-sarcoidosis-and-polymyositis
#15
Nobuyasu Awano, Kensuke Fukuda, Masashi Sakayori, Keisuke Kondoh, Ryu Ono, Atsuko Moriya, Tsunehiro Ando, Toshio Kumasaka, Tamiko Takemura, Soichiro Ikushima
We herein describe a case of sarcoid myositis with anti-Ku antibody positivity. Pathological findings of the muscle were compatible with sarcoidosis, but could not be completely distinguished from myositis diseases that arise from other causes. According to a physical examination, pathological findings, the detection of anti-Ku antibody and the human leukocyte antigen (HLA)-DPB1 allele, we strongly suspected that the patient developed both sarcoidosis and polymyositis. Sarcoidosis is often complicated by autoimmune diseases...
2016: Internal Medicine
https://www.readbyqxmd.com/read/27454740/multiplexed-and-scalable-super-resolution-imaging-of-three-dimensional-protein-localization-in-size-adjustable-tissues
#16
Taeyun Ku, Justin Swaney, Jeong-Yoon Park, Alexandre Albanese, Evan Murray, Jae Hun Cho, Young-Gyun Park, Vamsi Mangena, Jiapei Chen, Kwanghun Chung
The biology of multicellular organisms is coordinated across multiple size scales, from the subnanoscale of molecules to the macroscale, tissue-wide interconnectivity of cell populations. Here we introduce a method for super-resolution imaging of the multiscale organization of intact tissues. The method, called magnified analysis of the proteome (MAP), linearly expands entire organs fourfold while preserving their overall architecture and three-dimensional proteome organization. MAP is based on the observation that preventing crosslinking within and between endogenous proteins during hydrogel-tissue hybridization allows for natural expansion upon protein denaturation and dissociation...
September 2016: Nature Biotechnology
https://www.readbyqxmd.com/read/27438127/silk-encapsulated-plasmonic-biochips-with-enhanced-thermal-stability
#17
Congzhou Wang, Jingyi Luan, Sirimuvva Tadepalli, Keng-Ku Liu, Jeremiah J Morrissey, Evan D Kharasch, Rajesh R Naik, Srikanth Singamaneni
Because of their high sensitivity, cost-efficiency, and great potential as point-of-care biodiagnostic devices, plasmonic biosensors based on localized surface plasmon resonance have gained immense attention. However, most plasmonic biosensors and conventional bioassays rely on natural antibodies, which are susceptible to elevated temperatures and nonaqueous media. Hence, an expensive and cumbersome "cold chain" system is necessary to preserve the labile antibodies by maintaining optimal cold temperatures during transport, storage, and handling...
October 12, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27402064/pharmacokinetic-pharmacodynamic-and-safety-profile-of-a-novel-anti-cd28-domain-antibody-antagonist-in-healthy-subjects
#18
Rong Shi, Marek Honczarenko, Sean Zhang, Catherine Fleener, Johanna Mora, Sun Ku Lee, Reena Wang, Xiaoni Liu, Diane E Shevell, Zheng Yang, Haiqing Wang, Bindu Murthy
We report pharmacokinetics, pharmacodynamics, and safety of a novel anti-CD28 domain antibody antagonist (lulizumab pegol) in healthy subjects following single- or multiple-dose administration. A minimal anticipated biological effect level approach was used to select a 0.01 mg starting dose for a single-ascending-dose (SAD), double-blind, first-in-human study. Part 1 included 9 intravenous (IV; 0.01-100 mg) and 3 subcutaneous (SC; 9-50 mg) doses or placebo. In part 2, a keyhole limpet hemocyanin (KLH) immunization was performed in 16 subjects/panel, who received 1 of 3 IV doses (9-100 mg) or placebo...
July 12, 2016: Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/27349006/specific-ige-antibodies-in-young-children-with-atopic-dermatitis-correlation-of-multiple-allergen-simultaneous-immunoblot-test-and-immunocap-system
#19
Ewa Konopka, Aldona Ceregra, Elzbieta Maciorkowska, Barbara Surowska, Ilona Trojanowska, Izabela Roszko-Kirpsza, Bozena Cukrowska
BACKGROUND: Sensitization to food allergens is a common condition in pediatric atopic dermatitis (AD). Recently, the multiple allergen simultaneous test (MAST) allowing for a comprehensive assessment of atopy has been developed, but the usefulness in young AD children is not known. The aim of this study was to determine IgE specificity in AD children using MAST and to compare the results for selected food allergens with the reference ImmunoCap system. METHODS: The study enrolled 50 children up to 2 years old with a diagnosis of AD...
2016: Clinical Laboratory
https://www.readbyqxmd.com/read/27340093/virus-like-particle-based-vaccine-against-coxsackievirus-a6-protects-mice-against-lethal-infections
#20
Chaoyun Shen, Zhiqiang Ku, Yu Zhou, Dapeng Li, Lili Wang, Ke Lan, Qingwei Liu, Zhong Huang
Coxsackievirus A6 (CA6) is emerging as one of the major causative agents of hand, foot, and mouth disease (HFMD) worldwide. However, no vaccine is currently available for preventing CA6 infection. Here, we report the development of a virus-like particle (VLP)-based recombinant vaccine for CA6. We produced CA6 VLPs in insect cells by infecting the cells with a baculovirus coexpressing the genes encoding CA6 P1 and 3CD. Biochemical analyses showed that the produced VLPs consisted of VP0, VP1, and VP3 capsid subunit proteins generated by the cleavage of P1 by 3CD...
July 25, 2016: Vaccine
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