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Maite Arrizubieta, Oihane Simón, Trevor Williams, Primitivo Caballero
A co-occluded binary mixture of Helicoverpa armigera nucleopolyhedrovirus genotypes HearSP1B and HearLB6 at a 1:1 ratio (HearSP1B+HearLB6) was selected for the development of a virus-based biological insecticide, which requires an efficient large-scale production system. In vivo production systems require optimization studies in each host-virus pathosystem. In the present study, the effects of larval instar, rearing density, timing of inoculation, inoculum concentration and temperature on the production of HearSP1B+HearLB6 in its homologous host were evaluated...
2016: PloS One
Hongyu Mi, Mingming Guan, Hongyan Shan, Qiang Fei, Yanfu Huan, Zhiquan Zhang, Guodong Feng
A label-free, highly selective, and highly sensitive fluorescent sensor to detect Hg(2+) was developed using a water-soluble conjugated polymer with carboxylate groups (poly(2,5-bis(sodium 4-oxybutyrate)-1,4-phenylethynylene-alt-1,4-phenyleneethynylene, PPE-OBS) in this work. The fluorescence of PPE-OBS would be quenched because of the effect among the unique coordination-induced aggregation and electron transfers of PPE-OBS toward Hg(2+). The linear relationship between the fluorescence intensity and concentration of Hg(2+) was observed within the range from 6 × 10(-8) to 8 × 10(-5) mol L(-1) (R(2) = 0...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
Frederick J Ehlert, Richard Sl Stein
We describe a method for estimating the affinities of ligands for active and inactive states of a G protein-coupled receptor (GPCR). Our protocol involves measuring agonist-induced signaling responses of a wild type GPCR and a constitutively active mutant of it under control conditions and after partial receptor inactivation or reduced receptor expression. Our subsequent analysis is based on the assumption that the activating mutation increases receptor isomerization into the active state without affecting the affinities of ligands for receptor states...
October 7, 2016: Journal of Pharmacological and Toxicological Methods
Igor Diemberger, Giulia Massaro, Maria Letizia Bacchi Reggiani, Stefano Lorenzetti, Mauro Biffi, Matteo Ziacchi, Cristian Martignani, Giuseppe Boriani
Dronedarone was found to have divergent safety profiles in randomized controlled trials (RCT) in term of cardiac death and overall mortality. We decided to evaluate all available evidence on the cardiovascular safety of this drug. A systematic search was made of the MEDLINE and the Cochrane Central Register of Controlled Trials from January 2003 through April 2016 for RCT comparing dronedarone to placebo/active control, to provide the most accurate estimate of the effects of this agent and observational cohort studies (OBS) reporting clinical outcomes in patients treated with dronedarone, according to current guidelines, to obtain a real-life comparator for the findings summarized by RTC analysis...
October 6, 2016: Current Pharmaceutical Design
Romain Rivoirard, Vianney Duplay, Mathieu Oriol, Fabien Tinquaut, Franck Chauvin, Nicolas Magne, Aurelie Bourmaud
BACKGROUND: Quality of reporting for Randomized Clinical Trials (RCTs) in oncology was analyzed in several systematic reviews, but, in this setting, there is paucity of data for the outcomes definitions and consistency of reporting for statistical tests in RCTs and Observational Studies (OBS). The objective of this review was to describe those two reporting aspects, for OBS and RCTs in oncology. METHODS: From a list of 19 medical journals, three were retained for analysis, after a random selection: British Medical Journal (BMJ), Annals of Oncology (AoO) and British Journal of Cancer (BJC)...
2016: PloS One
David Gilbert
No abstract text is available yet for this article.
September 26, 2016: Medical Humanities
Goran Baranović
Three refractive index mixing rules, Arago-Biot, Lorentz-Lorenz, and Newton, are generalized to complex refractive index and used to define infrared (IR) spectra of the corresponding ideal liquid mixtures. Using the measured optical constants n and k for acetonitrile-water mixtures (Bertie and Lan, 1997) the excess absorbances, A(E )= A(obs) - A(ideal), are calculated. Relying upon the well-established properties of the acetonitrile-water mixtures, the interpretation of the excess absorbances is established that is essentially based on the understanding of a liquid as a set of oscillators...
September 19, 2016: Applied Spectroscopy
Marie Denizet, Laurent Cotter, Pierre-Marie Lledo, Françoise Lazarini
The olfactory bulb (OB) is a highly plastic structure that can change organizational networks depending on environmental inputs in adult mammals. Particularly, in rodents, adult neurogenesis underlies plastic changes in the OB circuitry by continuously adding new interneurons to the network. We addressed the question of whether microglia, the immune cells of the brain, were involved in pruning OB neurons. Using lentiviral labeling of neurons in neonatal or adult mice and confocal analysis, we showed that microglia engulfed parts of neonatal-born and adult-born neurons in the healthy OB...
September 15, 2016: Brain, Behavior, and Immunity
Rongbing Fu, Zhen Xu, Lin Peng, Dongsu Bi
In this study, nanoscale zerovalent iron (NZVI) immobilized on biomass carbon was used for the high efficient removal of BDE 209. NZVI supported on biomass carbon minimized the aggregation of NZVI particles resulting in the increased reaction performance. The proposed removal mechanism included the adsorption of BDE 209 on the surface or interior of the biomass carbon NZVI (BC-NZVI) particles and the subsequent debromination of BDE 209 by NZVI while biomass carbon served as an electron shuttle. BC-NZVI particles and the interaction between BC-NZVI particles and BDE 209 were characterized by TEM, XRD, and XPS...
September 16, 2016: Environmental Science and Pollution Research International
Ryuhei Kokusho, Yoshikazu Koh, Masaru Fujimoto, Toru Shimada, Susumu Katsuma
Bombyx mori nucleopolyhedrovirus (BmNPV) orf5 (Bm5) is a core gene of lepidopteran baculoviruses and encodes the protein with the conserved amino acid residues (DUF3627) in its C-terminus. Here, we found that Bm5 disruption resulted in lower titers of budded viruses and fewer numbers of occlusion bodies (OBs) in B. mori cultured cells and larvae, although viral genome replication was not affected. Bm5 disruption also caused aberrant expression of various viral genes at the very late stage of infection. Immunocytochemical analysis revealed that BM5 localized to the nuclear membrane...
November 2016: Virology
Ke Zhu, Pingping Song, Yumei Lai, Chuanju Liu, Guozhi Xiao
The roles of prolyl hydroxylase domain proteins (PHDs) in bone are incompletely understood. Here we deleted the expression of genes encoding PHD1, PHD2, and PHD3 in osteoblasts in mice by breeding the floxed Phd1-3 mice with Col1a1-Cre transgenic mice. Results showed that mice lacking PHD1-3 in osteoblasts (Phd1-3ob-/-) had increased bone mass. Bone parameters such as bone volume/tissue volume (BV/TV), trabecular number (Tb.N), and trabecular thickness (Tb.Th) were increased, while trabecular spacing (Tb.Sp) was decreased in Phd1-3ob-/- relative to wild-type (WT) femurs...
December 5, 2016: Gene
Aurélie Jacquart, Roberta Brayner, Jean-Michel El Hage Chahine, Nguyet-Thanh Ha-Duong
Phytochelatins or PCn, (γGlu-Cys)n-Gly, and their glutathione (GSH) precursor are thiol-rich peptides that play an important role in heavy metal detoxification in plants and microorganisms. Complex formation between Cd(2+) and Pb(2+) and GSH or PCn (n = 2, 4 and 6) are investigated by microcalorimetry, absorption spectrophotometry and T-jump kinetics. Complex formation with Pb(2+) or Cd(2+) is exothermic, and induces ligand metal charge transfer bands in UV absorption spectral range, which implies the formation of a coordination bond between the metal and the thiol groups of the phytochelatins...
September 6, 2016: Chemico-biological Interactions
S A Begum, T S Chowdhury, T Mahmud, S Chowdhury, T A Chowdhury, S J Urmi, S Khatun, A Nessa, N Fatema
A 25 years married women having one child delivered vaginaly presented in the department of Obs & Gynae, Bangabandhu Sheikh Mujib Medical University, Bangladesh on April 2013 with pelvic pain and discomfort. No history of previous pelvic trauma was present. Patient examination showed a isolated mass in the right lower abdomen, right adnexa, extending to the pelvic wall upto lower end of ureter. Tumor markers were within normal limit. Intravenous pyelogram (IVP) showed mild right hydroureter and hydronephrosis with obstruction at the lower end of ureter...
July 2016: Mymensingh Medical Journal: MMJ
Xu Yang, Hui Huo, Chunyu Xiu, Miao Song, Yanfei Han, Yanfei Li, Yanzhu Zhu
Bone morphogenetic protein-2 (BMP-2)/Smad signaling pathway plays an important role in regulating osteoblast (OB) differentiation. OB differentiation is a key process of bone formation. Aluminum (Al) exposure inhibits bone formation and causes Al-induced bone disease. However, the mechanism is not fully understood. To investigate whether BMP-2/Smad signaling pathway is associated with OB differentiation in aluminum trichloride (AlCl3)-treated OBs, the primary rat OBs were cultured and exposed to 0 (control group, CG), 1/40 IC50 (low-dose group, LG), 1/20 IC50 (mid-dose group, MG), and 1/10 IC50 (high-dose group, HG) of AlCl3 for 24 h, respectively...
September 4, 2016: Food and Chemical Toxicology
X Wu, N Z Kubilay, J Ren, B Allegranzi, P Bischoff, B Zayed, D Pittet, J Li
To investigate the effectiveness of antimicrobial-coated sutures compared with non-coated sutures in reducing surgical site infection (SSI) and develop recommendations for World Health Organization (WHO) SSI prevention guidelines. We searched Medline, Embase, Cinahl, Cochrane Central Register of Controlled Trials, and WHO Global Health from 1990-16/02/2015 with language restricted to English, Spanish, and French. Meta-analysis was performed with a random-effects model. Meta-regression analysis assessed whether the effect of antimicrobial coating changed according to the type of suture and surgery...
September 2, 2016: European Journal of Clinical Microbiology & Infectious Diseases
Mohammed A Al-Shargabi, Asadullah Shaikh, Abdulsamad S Ismail
Optical burst switching (OBS) networks have been attracting much consideration as a promising approach to build the next generation optical Internet. A solution for enhancing the Quality of Service (QoS) for high priority real time traffic over OBS with the fairness among the traffic types is absent in current OBS' QoS schemes. In this paper we present a novel Real Time Quality of Service with Fairness Ratio (RT-QoSFR) scheme that can adapt the burst assembly parameters according to the traffic QoS needs in order to enhance the real time traffic QoS requirements and to ensure the fairness for other traffic...
2016: PloS One
Bo Li, Ru-Yin Hu, Li Sun, Rui Luo, Kai-Hang Lu, Xiao-Bin Tian
The aim of the present study was to explore the effects of andrographolide (AG) and the ERK signaling pathway on the proliferation of osteoblasts (OBs) in vitro. The calvarial OBs from Sprague Dawley (SD) rats were collected and treated at different concentrations of AG and U0126. The concentrations of AG were measured by colorimetry. Based on different treatment methods, the cells were separated into four groups: control group, U0126 group, AG group, and AG+U0126 group. The cells were cultured for 24h, 48h and 72h...
August 26, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Xiaobing Huang, Biao Zhu, Xiaodong Wang, Rong Xiao, Chunsen Wang
Recent studies have indicated that the hematopoietic stem/progenitor cell (HSPC) niche, consisting of two major crucial components, namely osteoblasts (OBs) and mesenchymal stromal cells (MSCs), is responsible for the fate of HSPCs. Thus, closely mimicking the HSPC niche ex vivo may be an efficient strategy with which to develop new culture strategies to specifically regulate the balance between HSPC self-renewal and proliferation. The aim of this study was to establish a novel HSPC three-dimensional culture system by co-culturing bone marrow-derived MSCs and OBs differentiated from MSCs without any cytokines as feeder cells and applying bio-derived bone from human femoral metaphyseal portion as the scaffold...
October 2016: International Journal of Molecular Medicine
Fereshteh Shafiei, Mahtab Memarpour, Zahra Sarafraz
PURPOSE: This study was undertaken to investigate whether use of an adhesive penetration enhancer, dimethyl sulfoxide (DMSO), improves bond stability of fiber posts to root dentin using two two-step etch-and-rinse resin cements. MATERIALS AND METHODS: Forty human maxillary central incisor roots were randomly divided into 4 groups after endodontic treatment and post space preparation, based on the fiber post/cement used with and without DMSO pretreatment. Acid-etched root dentin was treated with 5% DMSO aqueous solution for 60 seconds or with distilled water (control) prior to the application of Excite DSC/Variolink II or One-Step Plus/Duo-link for post cementation...
August 2016: Journal of Advanced Prosthodontics
Lei Tan, Bin Liang, Wen Cheng, Zhanqiang Fang, Eric Pokeung Tsang
Nano zero-valent iron (nZVI) and its modified nanomaterials are widely used in the degradation of some halogenated organic pollutants. In this study, we explored the effects of different proportions of tetrahydrofuran (THF) (50, 60, 70, 80, 90, and 100 %) on the degradation of decabromodiphenyl ether (BDE209) by Ni/Fe and nZVI nanoparticles with reference to the degradation kinetics, products, and pathway. The results illustrated that the effects of solvent on the degradation of BDE209 were similar when the two kinds of nanomaterials were used, although the Ni/Fe bimetallic nanoparticles exhibited a better catalytic activity compared with the pure nZVI during the degradation of BDE209...
August 21, 2016: Environmental Science and Pollution Research International
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