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Dental stem cell

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https://www.readbyqxmd.com/read/28532101/sonochemical-synthesis-of-fructose-1-6-bisphosphate-dicalcium-porous-microspheres-and-their-application-in-promotion-of-osteogenic-differentiation
#1
Chao Qi, Ding Zhou, Ying-Jie Zhu, Tuan-Wei Sun, Feng Chen, Chang-Qing Zhang
Human bone mesenchymal stem cells (hBMSCs) have the ability to differentiate into bone and cartilage for clinical bone regeneration. Biomaterials with an innate ability to stimulate osteogenic differentiation of hBMSCs into bone and cartilage are considered attractive candidates for the applications in bone tissue engineering and regeneration. In this paper, we synthesized fructose 1,6-bisphosphate dicalcium (Ca2FBP) porous microspheres by the sonochemical method, and investigated the ability of Ca2FBP for the promotion of the osteogenic differentiation of hBMSCs...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531913/methods-of-isolation-and-characterization-of-stem-cells-from-different-regions-of-oral-cavity-using-markers-a-systematic-review
#2
REVIEW
Kavarthapu Avinash, Sankari Malaippan, Jayakumar Nadathur Dooraiswamy
Background: Periodontitis is a destructive inflammatory disorder of the periodontium caused by the destruction of periodontal tissues namely the PDL, cementum, alveolar bone, and gingiva. Once these tissues are lost, the foremost goal of periodontal therapy is to regenerate the diseased tissues if possible to their original form, architecture, and function. Various regenerative procedures were employed and still a gap was found in achieving the goal. As stem cells are characterized by their ability to self-renew and differentiate to produce specialized cells, there could be a possibility of using them for regenerative therapy...
May 30, 2017: International Journal of Stem Cells
https://www.readbyqxmd.com/read/28530652/cd34-cells-from-dental-pulp-stem-cells-with-a-zfn-mediated-and-homology-driven-repair-mediated-locus-specific-knock-in-of-an-artificial-%C3%AE-globin-gene
#3
S Chattong, O Ruangwattanasuk, W Yindeedej, A Setpakdee, K Manotham
In humans, mutations in the β-globin gene (HBB) have two important clinical manifestations: β-thalassemia and sickle cell disease. The progress in genome editing and stem cell research may be relevant to the treatment of β-globin-related diseases. In this work, we employed zinc finger nuclease (ZFN)-mediated gene integration of synthetic β-globin cDNA into HBB loci, thus correcting almost all β-globin mutations. The integration was achieved in both HEK 293 cells and isolated dental pulp stem cell (DPSCs)...
May 22, 2017: Gene Therapy
https://www.readbyqxmd.com/read/28530133/efficacy-of-a-self-assembling-peptide-hydrogel-spg-178-gel-for-bone-regeneration-and-three-dimensional-osteogenic-induction-of-dental-pulp-stem-cells
#4
Jun Tsukamoto, Keiko Naruse, Yusuke Nagai, Shuhei Kan, Nobuhisa Nakamura, Masaki Hata, Maiko Omi, Tatsuhide Hayashi, Tatsushi Kawai, Tatsuaki Matsubara
The aim of this study was to assess the efficacy of a self-assembling peptide hydrogel as a scaffold for bone regeneration. We used a neutral and injectable self-assembling peptide hydrogel, SPG-178-Gel. Bone defects (5 mm in diameter) in rat calvarial bones were filled with a mixture of alpha-modified Eagle's medium and the peptide hydrogel. Three weeks after surgery, soft X-ray and micro-computed tomography (micro-CT) images of the gel-treated bones showed new bone formations in the periphery and in central areas of the defects...
May 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28527850/complement-activation-by-pulp-capping-materials-plays-a-significant-role-in-both-inflammatory-and-pulp-stem-cells-recruitment
#5
Thomas Giraud, Pierre Rufas, Fanny Chmilewsky, Charlotte Rombouts, Jacques Dejou, Charlotte Jeanneau, Imad About
INTRODUCTION: The role of complement, especially through the C5a fragment, is well-known for the initiation of inflammation. Its involvement in regeneration has been shown more recently by the recruitment of mesenchymal stem cells. C5a can be produced locally by the pulp fibroblasts in response to injury or infection. This work aims to investigate the effect of different pulp capping biomaterials on complement activation and its possible influence on inflammatory and pulp stem cell recruitment...
May 17, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28527849/role-of-piezo-channels-in-ultrasound-stimulated-dental-stem-cells
#6
Qianhua Gao, Paul R Cooper, A Damien Walmsley, Ben A Scheven
INTRODUCTION: Piezo1 and Piezo2 are mechanosensitive membrane ion channels. We hypothesized that Piezo proteins may play a role in transducing ultrasound-associated mechanical signals and activate downstream mitogen-activated protein kinase (MAPK) signaling processes in dental cells. In this study, the expression and role of Piezo channels were investigated in dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) after treatment with low-intensity pulsed ultrasound (LIPUS)...
May 17, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28506888/calcium-sensing-receptor-erk-signaling-promotes-odontoblastic-differentiation-of-human-dental-pulp-cells
#7
Hiroyuki Mizumachi, Shinichiro Yoshida, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Asuka Yuda, Hideki Sugii, Suguru Serita, Hiromi Mitarai, Katsuaki Koori, Naohisa Wada, Hidefumi Maeda
Activation of the G protein-coupled calcium-sensing receptor (CaSR) has crucial roles in skeletal development and bone turnover. Our recent study has identified a role for activated CaSR in the osteogenic differentiation of human periodontal ligament stem cells. Furthermore, odontoblasts residing inside the tooth pulp chamber play a central role in dentin formation. However, it remains unclear how CaSR activation affects the odontoblastic differentiation of human dental pulp cells (HDPCs). We have investigated the odontoblastic differentiation of HDPCs exposed to elevated levels of extracellular calcium (Ca) and strontium (Sr), and the contribution of CaSR and the L-type voltage-dependent calcium channel (L-VDCC) to this process...
May 12, 2017: Bone
https://www.readbyqxmd.com/read/28503150/dental-pulp-cells-isolated-from-teeth-with-superficial-caries-retain-an-inflammatory-phenotype-and-display-an-enhanced-matrix-mineralization-potential
#8
Hanaa Alkharobi, James Beattie, Josie Meade, Deirdre Devine, Reem El-Gendy
We have isolated dental pulp cells (DPCs) from three healthy (hDPCs) and three carious (cDPCs) donors and shown that compared to hDPCs cells isolated from superficial carious lesions show higher clonogenic potential; show an equivalent proportion of cells with putative stem cell surface markers; show enhanced matrix mineralization capability; have enhanced angiogenic marker expression and retain the inflammatory phenotype in vitro characteristic of superficial caries lesions in vivo. Our findings suggest that cDPCs may be used for further investigation of the cross talk between inflammatory, angiogenic and mineralization pathways in repair of carious pulp...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28500952/isolation-and-characterization-of-lymphoid-enhancer-factor-1-positive-deciduous-dental-pulp-stem-like-cells-after-transfection-with-a-piggybac-vector-containing-lef1-promoter-driven-selection-markers
#9
Tomoya Murakami, Issei Saitoh, Masahiro Sato, Emi Inada, Miki Soda, Masataka Oda, Hisanori Domon, Yoko Iwase, Tadashi Sawami, Kazunari Matsueda, Yutaka Terao, Hayato Ohshima, Hirofumi Noguchi, Haruaki Hayasaki
OBJECTIVE: Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. LEF1 is also an important member of the Wnt/β-catenin signaling pathway that plays important roles in the self-renewal and differentiation of embryonic stem cells. We speculated that LEF1 might function in the stem cells from human exfoliated deciduous teeth (SHED). In this study, we attempted to isolate such LEF1-positive cells from human deciduous dental pulp cells (HDDPCs) by genetic engineering technology, using the human LEF1 promoter...
May 7, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28498451/differential-effects-of-p38-and-erk1-2-on-the-chondrogenic-and-osteogenic-differentiation-of-dental-pulp-stem-cells
#10
Pengfei Ba, Xiaoyu Duan, Guo Fu, Shuyan Lv, Pishan Yang, Qinfeng Sun
The extracellular signal-regulated protein kinase 1/2 (Erk1/2) and p38 mitogen‑activated protein‑kinase pathways serve important roles in the regulation of osteogenic and chondrogenic differentiation in mesenchymal stem cells (MSCs). However, the exact mechanism remains unclear, and the effect is controversial. In the present study, the effects of Erk1/2 and p38 on the osteogenic and chondrogenic differentiation of dental pulp stem cells (DPSCs) were compared in vitro. The results indicated that inhibition of Erk1/2 is able to enhance the osteogenic differentiation of DPSCs and inhibit chondrogenic differentiation, whereas inhibition of p38 demonstrated the opposite effect...
May 10, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28496518/cytotoxicity-of-selected-nanoparticles-on-human-dental-pulp-stem-cells
#11
Kasra Tabari, Sepanta Hosseinpour, Peter Parashos, Parisa Kardouni Khozestani, Hossein Mohammad Rahimi
INTRODUCTION: Nanoparticles are being increasingly applied in dentistry due to their antimicrobial and mechanical properties. This in vitro study aimed to assess and compare the cytotoxicity of four metal oxide nanoparticles (TiO2, SiO2, ZnO, and Al2O3) on human dental pulp stem cells. METHODS AND MATERIALS: Four suspension with different concentrations (25, 50, 75, 100 µg/mL) of each nanoparticle were prepared and placed into cavities of three 96-well plates (containing 1×10(4) cells per well that were seeded 24 earlier)...
2017: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/28495017/graphene-for-the-development-of-the-next-generation-of-biocomposites-for-dental-and-medical-applications
#12
REVIEW
Han Xie, Tong Cao, Francisco Javier Rodríguez-Lozano, Emma Kim Luong-Van, Vinicius Rosa
OBJECTIVE: Graphene and its derivatives, graphene oxide (GO) and reduced graphene oxide (rGO), are 2D carbon-based materials with remarkable physical, chemical and biological properties. Graphene sheets have high specific surface area and mechanical strength. Moreover, they have been shown to influence the differentiation of stem cells and to improve properties of biomaterials. METHODS: Here, we present the recent achievements on the use of graphene and its derivatives to improve properties and enhance bioactivity of biomaterials...
May 8, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28494644/the-alpha-7-nicotinic-acetylcholine-receptor-of-deciduous-dental-pulp-stem-cells-regulates-osteoclastogenesis-during-physiological-root-resorption
#13
Lulu Wang, Zhifei Zhou, Yujiang Chen, Shuai Yuan, Yang Du, Xinke Ju, Lizheng Wu, Xiaojing Wang
The physiological root resorption of deciduous teeth is a normal phenomenon, but the mechanisms underlying this process are still unclear. In this study, deciduous dental pulp stem cells (DDPSCs) and permanent dental pulp stem cells (DPSCs) were derived from deciduous teeth and normal permanent teeth at different stages of resorption. In the middle stage of root resorption, DDPSCs exhibited an increase in the ability to induce osteoclast differentiation. Activation of the alpha 7 nicotinic acetylcholine receptor (α7 nAChR) by secretory mammalian Ly-6 urokinase-type plasminogen activator receptor-associated protein 1 (SLURP-1) caused a significant increase in the expression levels of NF-κB, RANKL and the ratio of RANKL/OPG...
May 11, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28491933/wnt-and-bmp-signaling-crosstalk-in-regulating-dental-stem-cells-implications-in-dental-tissue-engineering
#14
Fugui Zhang, Jinglin Song, Hongmei Zhang, Enyi Huang, Dongzhe Song, Viktor Tollemar, Jing Wang, Jinhua Wang, Maryam Mohammed, Qiang Wei, Jiaming Fan, Junyi Liao, Yulong Zou, Feng Liu, Xue Hu, Xiangyang Qu, Liqun Chen, Xinyi Yu, Hue H Luu, Michael J Lee, Tong-Chuan He, Ping Ji
Tooth is a complex hard tissue organ and consists of multiple cell types that are regulated by important signaling pathways such as Wnt and BMP signaling. Serious injuries and/or loss of tooth or periodontal tissues may significantly impact aesthetic appearance, essential oral functions and the quality of life. Regenerative dentistry holds great promise in treating oral/dental disorders. The past decade has witnessed a rapid expansion of our understanding of the biological features of dental stem cells, along with the signaling mechanisms governing stem cell self-renewal and differentiation...
December 2016: Genes & Diseases
https://www.readbyqxmd.com/read/28487958/effects-of-taz-on-human-dental-pulp-stem-cell-proliferation-and-migration
#15
Songbo Tian, Xiaochao Tian, Yanping Liu, Fusheng Dong, Jie Wang, Xuqian Liu, Zhiyong Zhang, Huizhen Chen
Transcriptional coactivator with PDZ‑binding motif (TAZ) acts as the key downstream regulatory target in the Hippo signaling pathway. TAZ overexpression has been reported to promote cellular proliferation and induce epithelial‑mesenchymal transition in human mammary epithelial cells. However, the effects of TAZ in the regulation of human dental pulp stem cell (hDPSC) proliferation and migration, as well as the molecular mechanisms underlying its actions, remain to be elucidated. The present study demonstrated that TAZ was expressed in hDPSCs...
June 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28484278/role-of-gata-binding-protein-4-gata4-in-the-regulation-of-tooth-development-via-gnai3
#16
Shuyu Guo, Yuxin Zhang, Tingting Zhou, Dongyue Wang, Yajuan Weng, Lin Wang, Junqing Ma
Transcription factor GATA4 regulates cardiac and osteoblast differentiation. However, its role in tooth development is not clear. Therefore, we generated Wnt1-Cre;GATA4 (fl/fl) mice, with conditional inactivation of the GATA4 gene in the dental papilla mesenchymal cells. Phenotypic analysis showed short root deformity along with reduced expressions of odonto/osteogenic markers. Proliferation (but not apoptosis) of cells around the apical area of the root was attenuated. In vitro, we knocked down GATA4 expression in stem cells of dental apical papilla (SCAPs)...
May 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28482579/tio2-nanorod-arrays-modified-ti-substrates-promote-the-adhesion-proliferation-and-osteogenic-differentiation-of-human-periodontal-ligament-stem-cells
#17
Zhi Li, Jichuan Qiu, Ling Qian Du, Lu Jia, Hong Liu, Shaohua Ge
Nanostructure coating on titanium (Ti) implants is well known as a cue for directing osteoblast behavior and function. However, effects of nanostructure coatings on dental stem cells have been rarely explored. In this work, assembled TiO2 nanorod arrays (TNRs) were fabricated on the polished Ti substrates using hydrothermal and sintering methods. The adhesion, morphology, proliferation and osteogenic differentiation of human periodontal ligament stem cells (PDLSCs) seeded onto TNRs substrates were evaluated...
July 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28481351/dental-status-does-not-predict-infection-during-stem-cell-transplantation-a-single-center-survey
#18
A Guenther, E Losch, M Schiessl, A Schrauder, A Humpe, R Repp, T Nitsche, J Wiltfang, M Gramatzki
No abstract text is available yet for this article.
May 8, 2017: Bone Marrow Transplantation
https://www.readbyqxmd.com/read/28479933/the-potential-of-enriched-mesenchymal-stem-cells-with-neural-crest-cell-phenotypes-as-a-cell-source-for-regenerative-dentistry
#19
REVIEW
Kunimichi Niibe, Maolin Zhang, Kosuke Nakazawa, Satoru Morikawa, Taneaki Nakagawa, Yumi Matsuzaki, Hiroshi Egusa
Effective regenerative treatments for periodontal tissue defects have recently been demonstrated using mesenchymal stromal/stem cells (MSCs). Furthermore, current bioengineering techniques have enabled de novo fabrication of tooth-perio dental units in mice. These cutting-edge technologies are expected to address unmet needs within regenerative dentistry. However, to achieve efficient and stable treatment outcomes, preparation of an appropriate stem cell source is essential. Many researchers are investigating the use of adult stem cells for regenerative dentistry; bone marrow-derived MSCs (BM-MSCs) are particularly promising and presently used clinically...
May 2017: Japanese Dental Science Review
https://www.readbyqxmd.com/read/28475038/resolving-stem-and-progenitor-cells-in-the-adult-mouse-incisor-through-gene-co-expression-analysis
#20
Kerstin Seidel, Pauline Marangoni, Cynthia Tang, Bahar Houshmand, Wen Du, Richard L Maas, Steven Murray, Michael C Oldham, Ophir D Klein
Investigations into stem cell-fueled renewal of an organ benefit from an inventory of cell type-specific markers and a deep understanding of the cellular diversity within stem cell niches. Using the adult mouse incisor as a model for a continuously renewing organ, we performed an unbiased analysis of gene co-expression relationships to identify modules of co-expressed genes that represent differentiated cells, transit-amplifying cells, and residents of stem cell niches. Through in vivo lineage tracing, we demonstrated the power of this approach by showing that co-expression module members Lrig1 and Igfbp5 define populations of incisor epithelial and mesenchymal stem cells...
May 5, 2017: ELife
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