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Dental stem cell

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https://www.readbyqxmd.com/read/28106508/gelma-encapsulated-hdpscs-and-huvecs-for-dental-pulp-regeneration
#1
A Khayat, N Monteiro, E E Smith, S Pagni, W Zhang, A Khademhosseini, P C Yelick
Pulpal revascularization is commonly used in the dental clinic to obtain apical closure of immature permanent teeth with thin dentinal walls. Although sometimes successful, stimulating bleeding from the periapical area of the tooth can be challenging and in turn may deleteriously affect tooth root maturation. Our objective here was to define reliable methods to regenerate pulp-like tissues in tooth root segments (RSs). G1 RSs were injected with human dental pulp stem cells (hDPSCs) and human umbilical vein endothelial cells (HUVECs) encapsulated in 5% gelatin methacrylate (GelMA) hydrogel...
February 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28106505/pulp-vascularization-during-tooth-development-regeneration-and-therapy
#2
C Rombouts, T Giraud, C Jeanneau, I About
The pulp is a highly vascularized tissue situated in an inextensible environment surrounded by rigid dentin walls, with the apical foramina being the only access. The pulp vascular system is not only responsible for nutrient supply and waste removal but also contributes actively to the pulp inflammatory response and subsequent regeneration. This review discusses the underlying mechanisms of pulp vascularization during tooth development, regeneration, and therapeutic procedures, such as tissue engineering and tooth transplantation...
February 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28105979/human-dental-pulp-stem-cells-are-more-effective-than-human-bone-marrow-derived-mesenchymal-stem-cells-in-cerebral-ischemic-injury
#3
Miyeoun Song, Jae-Hyung Lee, Jinhyun Bae, Youngmin Bu, Eun-Cheol Kim
We compared the therapeutic effects and mechanism of transplanted human dental pulp stem cells (hDPSCs) and human bonemarrow-derived mesenchymal stem cells (hMSCs) in a stroke model of rats and an <i>in vitro</i> ischemic model. Both hDPSCs- and hMSCs- intravenously injected rats 24&#8201;h after middle cerebral artery occlusion showed improved functional recovery and reduced infarct volume versus control rats, but the hDPSCs group showed greater reduction in infarct volume than hMSCs. The positive area for the endothelial cell marker was greater in the lesion boundary areas in the hDPSCs group than with hMSCs...
January 20, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28098169/ifn-%C3%AE-regulates-human-dental-pulp-stem-cells-behavior-via-nf-%C3%AE%C2%BAb-and-mapk-signaling
#4
Xinyao He, Wenkai Jiang, Zhirong Luo, Tiejun Qu, Zhihua Wang, Ningning Liu, Yaqing Zhang, Paul R Cooper, Wenxi He
During caries, dental pulp expresses a range of pro-inflammatory cytokines in response to the infectious challenge. Interferon gamma (IFN-γ) is a dimerized soluble cytokine, which is critical for immune responses. Previous study has demonstrated that IFN-γ at relative high concentration (100 ng/mL) treatment improved the impaired dentinogenic and immunosuppressive regulatory functions of disease-derived dental pulp stem cells (DPSCs). However, little is known about the regulatory effects of IFN-γ at relative low concentration on healthy DPSC behavior (including proliferation, migration, and multiple-potential differentiation)...
January 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28094306/maternal-diabetes-modulates-dental-epithelial-stem-cells-proliferation-and-self-renewal-in-offspring-through-apurinic-apyrimidinicendonuclease-1-mediated-dna-methylation
#5
Guoqing Chen, Jie Chen, Zhiling Yan, Ziyue Li, Mei Yu, Weihua Guo, Weidong Tian
Maternal gestational diabetes mellitus (GDM) has many adverse effects on the development of offspring. Aberrant DNA methylation is a potential mechanism associated with these effects. However, the effects of GDM on tooth development and the underlying mechanisms have not been thoroughly investigated. In the present study, a GDM rat model was established and incisor labial cervical loop tissue and dental epithelial stem cells (DESCs) were harvested from neonates of diabetic and control dams. GDM significantly suppressed incisor enamel formation and DESCs proliferation and self-renewal in offspring...
January 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28088458/non-thermal-atmospheric-pressure-plasma-functionalized-dental-implant-for-enhancement-of-bacterial-resistance-and-osseointegration
#6
Jung-Hwan Lee, Won-Seok Jeong, Seog-Jin Seo, Hae-Won Kim, Kyoung-Nam Kim, Eun-Ha Choi, Kwang-Mahn Kim
OBJECTIVE: Even though roughened titanium (Ti) and Ti alloys have been clinically used as dental implant, they encourage bacterial adhesion, leading to failure of the initial stability. Here, the non-thermal atmospheric pressure plasma jet (NTAPPJ) functionalized Ti and Ti alloy were investigated to promote cellular activities but inhibit the initial attachment of the adherent pioneer bacterium, Streptococcus sanguinis, without topographical changes. METHODS: After the produced radicals from NTAPPJ were characterized, bacterial adhesion to specimens was assessed by PrestoBlue assay and live-dead staining with or without the NTAPPJ functionalizing...
January 11, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28081373/loss-of-function-of-evc2-in-dental-mesenchyme-leads-to-hypomorphic-enamel
#7
H Zhang, H Takeda, T Tsuji, N Kamiya, T Kunieda, Y Mochida, Y Mishina
Ellis-van Creveld (EvC) syndrome is an autosomal-recessive skeletal dysplasia, characterized by short stature and postaxial polydactyly. A series of dental abnormalities, including hypomorphic enamel formation, has been reported in patients with EvC. Despite previous studies that attempted to uncover the mechanism leading to abnormal tooth development, little is known regarding how hypomorphic enamel is formed in patients with EvC. In the current study, using Evc2/ Limbin mutant mice we recently generated, we analyzed enamel formation in the mouse incisor...
December 1, 2016: Journal of Dental Research
https://www.readbyqxmd.com/read/28079995/effects-of-cell-cycle-phases-on-the-induction-of-dental-pulp-stem-cells-toward-dopaminergic-like-cells
#8
Nareshwaran Gnanasegaran, Vijayendran Govindasamy, Premasangery Kathirvaloo, Sabri Musa, Noor Hayaty Abu Kasim
Parkinson's disease (PD) is characterized by tremors and cognitive issues, and is due to the death of dopaminergic (DA-ergic) neurons in brain circuits that are responsible for producing neurotransmitter dopamine (DA). Currently, cell replacement therapies are underway to overcome existing therapeutic approaches such as drug treatments and electrical stimulation. Among the widely available sources, dental pulp stem cells from deciduous teeth (DPSC) have gained popularity due to their neural crest origin and inherent propensity toward neuronal lineage...
January 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28079309/bioactive-injectable-aggregates-with-nanofibrous-microspheres-and-human-dental-pulp-stem-cells-a-translational-strategy-in-dental-endodontics
#9
I Garzón, M A Martin-Piedra, V Carriel, M Alaminos, X Liu, R D Souza
Regeneration of the pulp-dentin complex with stem cells is a potential alternative to conventional root canal treatments. Human dental pulp stem cells (hDPSCs) have been extensively studied because of their ability to proliferate and differentiate into mineralized dental and nondental tissues. In this work we combined hDPSCs with two types of injectable poly-L-lactic acid (PLLA) microspheres with a nanofibrous or smooth surface to form bioactive injectable aggregates, and examined their ability to promote pulp regeneration in the root canal in an in vivo model...
January 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28076902/decellularized-extracellular-matrix-of-human-umbilical-vein-endothelial-cells-promotes-endothelial-differentiation-of-stem-cells-from-exfoliated-deciduous-teeth
#10
Ting Gong, Boon Chin Heng, Jianguang Xu, Shaoyue Zhu, Changyong Yuan, Edward Chin Man Lo, Chengfei Zhang
Dental stem cells can serve as a potential source of functional endothelial cells (ECs) for tissue engineering applications, but the endothelial-lineage differentiation efficiency is rather low even with growth factors and mechanical stimuli, which greatly limits their clinical applications. This is partly due to the deficiency of standard two-dimensional (2-D) culture systems, which is unable to recapitulate the three-dimensional (3-D) in vivo milieu that is rich in extracellular matrix. Hence we extracted decellularized extracellular matrix from human umbilical vein endothelial cells (HUVECs-DECM) to provide a bioactive substratum conducive to the endothelial differentiation of dental stem cells...
January 11, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28075485/culturing-and-neuronal-differentiation-of-human-dental-pulp-stem-cells
#11
Sarita Goorha, Lawrence T Reiter
A major issue in studying human neurogenetic disorders, especially rare syndromes affecting the nervous system, is the ability to grow neuronal cultures that accurately represent these disorders for analysis. Although there has been some success in generating induced pluripotent stem (iPS) cells from both skin and blood, there are still limitations to the collection and production of iPS cells from these biospecimens. We have had significant success in collecting and growing human dental pulp stem (DPS) cells from exfoliated teeth sent to our laboratory by the parents of children with a variety of rare neurogenetic syndromes...
January 11, 2017: Current Protocols in Human Genetics
https://www.readbyqxmd.com/read/28070691/biological-characteristic-effects-of-human-dental-pulp-stem-cells-on-poly-%C3%AE%C2%B5-caprolactone-biphasic-calcium-phosphate-fabricated-scaffolds-using-modified-melt-stretching-and-multilayer-deposition
#12
Natkrita Wongsupa, Thongchai Nuntanaranont, Suttatip Kamolmattayakul, Nuttawut Thuaksuban
Craniofacial bone defects such as alveolar cleft affect the esthetics and functions that need bone reconstruction. The advanced techniques of biomaterials combined with stem cells have been a challenging role for maxillofacial surgeons and scientists. PCL-coated biphasic calcium phosphate (PCL-BCP) scaffolds were created with the modified melt stretching and multilayer deposition (mMSMD) technique and merged with human dental pulp stem cells (hDPSCs) to fulfill the component of tissue engineering for bone substitution...
February 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28067275/sdf-1-cxcr4-axis-induces-human-dental-pulp-stem-cell-migration-through-fak-pi3k-akt-and-gsk3%C3%AE-%C3%AE-catenin-pathways
#13
Mingwei Li, Xuefei Sun, Liang Ma, Lu Jin, Wenfei Zhang, Min Xiao, Qing Yu
SDF-1 (stromal cell derived factor-1) has been found to be widely expressed during dental pulp inflammation, while hDPSCs (human dental pulp stem cells) contribute to the repair of dental pulp. We showed that the migration of hDPSCs was induced by SDF-1 in a concentration-dependent manner and could be inhibited with siCXCR4 (C-X-C chemokine receptor type 4) and siCDC42 (cell division control protein 42), as well as drug inhibitors such as AMD3100 (antagonist of CXCR4), LY294002 (inhibitor of PI3K) and PF573228 (inhibitor of FAK)...
January 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28053317/jawbone-microenvironment-promotes-periodontium-regeneration-by-regulating-the-function-of-periodontal-ligament-stem-cells
#14
Bin Zhu, Wenjia Liu, Yihan Liu, Xicong Zhao, Hao Zhang, Zhuojing Luo, Yan Jin
During tooth development, the jawbone interacts with dental germ and provides the development microenvironment. Jawbone-derived mesenchymal stem cells (JBMSCs) maintain this microenvironment for root and periodontium development. However, the effect of the jawbone microenvironment on periodontium tissue regeneration is largely elusive. Our previous study showed that cell aggregates (CAs) of bone marrow mesenchymal stem cells promoted periodontium regeneration on the treated dentin scaffold. Here, we found that JBMSCs enhanced not only the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) but also their adhesion to titanium (Ti) material surface...
January 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28052540/engineered-neural-tissue-with-schwann-cell-differentiated-human-dental-pulp-stem-cells-potential-for-peripheral-nerve-repair
#15
Kathleen Sanen, Wendy Martens, Melanie Georgiou, Marcel Ameloot, Ivo Lambrichts, James Phillips
Despite the spontaneous regenerative capacity of the peripheral nervous system, large gap peripheral nerve injuries (PNIs) require bridging strategies. The limitations and suboptimal results obtained with autografts or hollow nerve conduits in the clinic urge the need for alternative treatments. Recently, we have described promising neuroregenerative capacities of Schwann cells derived from differentiated human dental pulp stem cells (d-hDPSCs) in vitro. Here, we extended the in vitro assays to show the pro-angiogenic effects of d-hDPSCs, such as enhanced endothelial cell proliferation, migration and differentiation...
January 4, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28043012/tnf-alpha-stimulation-increases-dental-pulp-stem-cell-migration-in-vitro-through-integrin-alpha-6-subunit-upregulation
#16
Lei Shi, Shanqi Fu, Sidra Fahim, Shuang Pan, He Lina, Xiaodan Mu, Yumei Niu
OBJECTIVE: The dissemination of stem cells into tissues requiring inflammatory and reparative response is fundamentally dependent upon their chemotactic migration. Expression of TNF-α is up regulated in inflamed pulps. Dental pulp cells are also known to express integrin α6 subunit. Expression of integrin subunit α6 has been linked to the acquisition of migratory potential in a wide variety of cell types in both pathological and physiological capacities. Therefore, in this study we examined the effects of a pleiotropic cytokine TNF-α on the migration of hDPSCs and investigated its relationship with expression of integrin α6 in hDPSCs during chemotactic migration...
December 15, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/28042545/comparative-in-vitro-evaluation-of-human-dental-pulp-and-follicle-stem-cell-commitment
#17
Razieh Karamzadeh, Mohamadreza Baghaban Eslaminejad, Ali Sharifi-Zarchi
OBJECTIVE: Pulp and periodontal tissues are well-known sources of mesenchymal stem cells (MSCs) that provide a promising place in tissue engineering and regenerative medicine. The molecular mechanisms underlying commitment and differentiation of dental stem cells that originate from different dental tissues are not fully understood. In this study, we have compared the expression levels of pluripotency factors along with immunological and developmentally-related markers in the culture of human dental pulp stem cells (hDPSCs), human dental follicle stem cells (hDFSCs), and human embryonic stem cells (hESCs)...
2017: Cell Journal
https://www.readbyqxmd.com/read/28041686/evaluation-of-a-commercially-available-hyaluronic-acid-hydrogel-restylane-as-injectable-scaffold-for-dental-pulp-regeneration-an-in%C3%A2-vitro-evaluation
#18
Vanessa Chrepa, Obadah Austah, Anibal Diogenes
INTRODUCTION: Regenerative endodontic procedures (REPs) are viable alternatives for treating immature teeth, yet these procedures do not predictably lead to pulp-dentin regeneration. A true bioengineering approach for dental pulp regeneration requires the incorporation of a scaffold conducive with the regeneration of the pulp-dentin complex. Several materials have been proposed as scaffolds for REPs; nonetheless, the majority are not eligible for immediate clinical chairside use. Thus, the aim of this study was to evaluate Restylane, a Food and Drug Administration-approved hyaluronic acid-based gel, as possible scaffold for REPs...
December 29, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/28033183/investigation-of-osteoinductive-effects-of-different-compositions-of-bioactive-glass-nanoparticles-for-bone-tissue-engineering
#19
AmirHossein Tavakolizadeh, Mehdi Ahmadian, MohammadHossein Fathi, Ali Doostmohammadi, Ehsan Seyedjafari, Abdolreza Ardeshirylajimi
Bioactive glasses (BG) is one of the well-known materials that used as dental and bone implants, for this reason it is always interesting for researchers has been to increase BG efficiency in the bone tissue engineering. The aim of this work was to evaluate the osteoinductivity of BG different composition nanoparticles with SiO2-CaO-P2O5. The 45S, 58S, and 63S compositions were prepared via the sol-gel technique. Characterization techniques such as X-ray diffraction, field emission scanning electron microscopy (FE-SEM), and laser Doppler electrophoresis (LDE) were used...
December 26, 2016: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/28033065/effects-of-bioactive-compounds-on-odontogenic-differentiation-and-mineralization
#20
S Phung, C Lee, C Hong, M Song, J K Yi, R G Stevenson, M K Kang, K-H Shin, N-H Park, R H Kim
Direct pulp capping involves the placement of dental materials directly onto vital pulp tissues after deep caries removal to stimulate the regeneration of reparative dentin. This physical barrier will serve as a "biological seal" between these materials and the pulp tissue. Although numerous direct pulp capping materials are available, the use of small bioactive compounds that can potently stimulate and expedite reparative dentin formation is still underexplored. Here, the authors compared and evaluated the pro-osteogenic and pro-odontogenic effects of 4 small bioactive compounds- phenamil (Phen), purmorphamine (Pur), genistein (Gen), and metformin (Met)...
January 2017: Journal of Dental Research
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