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https://www.readbyqxmd.com/read/28927137/atypical-chronic-myeloid-leukemia-with-isochromosome-x-p10-a-case-report
#1
Masahide Yamamoto, Sayaka Suzuki, Jun-Ichi Mukae, Keisuke Tanaka, Ken Watanabe, Gaku Oshikawa, Tetsuya Fukuda, Naomi Murakami, Osamu Miura
Atypical chronic myeloid leukemia (aCML) is a rare subtype of myelodysplastic/myeloproliferative neoplasm (MDS/MPN). Although recurrent chromosomal and genetic abnormalities are frequently observed in aCML, none are specific to this type of leukemia. The present study reported a case of aCML associated with i(X)(p10), a rare recurrent chromosomal abnormality of hematological malignancy. A 40-year-old female was referred to the Tokyo Medical and Dental University Hospital (Tokyo, Japan) due to slight leukocytosis and anemia...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28914948/nanotopography-mediated-osteogenic-differentiation-of-human-dental-pulp-derived-stem-cells
#2
Akash Bachhuka, Bahman Delalat, Soraya Rasi Ghaemi, Stan Gronthos, Nicolas H Voelcker, Krasimir Vasilev
Advanced medical devices, treatments and therapies demand an understanding of the role of interfacial properties on the cellular response. This is particularly important in the emerging fields of cell therapies and tissue regeneration. In this study, we evaluate the role of surface nanotopography on the fate of human dental pulp derived stem cells (hDPSC). These stem cells have attracted interest because of their capacity to differentiate to a range of useful lineages but are relatively easy to isolate. We generated and utilized density gradients of gold nanoparticles which allowed us to examine, on a single substrate, the influence of nanofeature density and size on stem cell behavior...
September 15, 2017: Nanoscale
https://www.readbyqxmd.com/read/28910136/nrp1-accelerates-odontoblast-differentiation-of-dental-pulp-stem-cells-through-classical-wnt-%C3%AE-catenin-signaling
#3
Yihua Song, Xiaojuan Liu, Xingmei Feng, Zhifeng Gu, Yongchun Gu, Min Lian, Jingwen Xiao, Peipei Cao, Ke Zheng, Xiaobing Gu, Dongping Li, Ping He, Chenfei Wang
Neuropilin-1 (NRP1) is one of the members of neuropilin family. It can combine with disparate ligands involved in regulating cell proliferation, apoptosis, and differentiation. The binding of NRP1 to Sema3A stimulates osteoblast differentiation through the classical Wnt/β-catenin pathway. However, the functions of NRP1 in dental pulp stem cells (DPSCs) are not clear. The aim of our study was to investigate how NRP1 controlled odontoblast differentiation in DPSCs and clarified the underlying mechanisms. NRP1 expression was increased in time-dependent manner along with cell odontoblast differentiation...
September 14, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28902344/insulin-like-growth-factor-1-promotes-the-proliferation-and-odontoblastic-differentiation-of-human-dental-pulp-cells-under-high-glucose-conditions
#4
Lu Yan, Shangmin Sun, Liu Qu
Insulin-like growth factor-1 (IGF-1) promotes human dental pulp stem cell proliferation and osteogenic differentiation. However, the effects of IGF-1 on the proliferation, apoptosis and odontoblastic differentiation (mineralization) of dental pulp cells (DPCs) under high glucose (GLU) conditions remain unclear. In this study, isolated primary human DPCs were treated with various concentrations of high GLU. Cell proliferation and apoptosis were determined by Cell Counting Kit-8 and Annexin V-FITC/PI assays, respectively...
September 1, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28901252/the-origin-and-identification-of-mesenchymal-stem-cells-in-teeth-from-odontogenic-to-non-odontogenic
#5
Dixin Cui, Hongyu Li, Mian Wan, Yiran Peng, Xin Xu, Xuedong Zhou, Liwei Zheng
Mesenchymal stem cells (MSCs) in teeth have been exploited as vital seed cells for stem cell-based dental medicine. To date, several mesenchymal stem cell populations originated from odontogenic tissue have been isolated and characterized by their expression of MSC surface markers and capacity of multi-lineage differentiation, including dental pulp stem cells (DPSCs), stem cells from human exfoliated deciduous teeth (SHED), stem cells from apical papilla (SCAP) and so on. However, their identity in vivo remains elusive, which hinders further understanding of their application in stem cell-based tooth regeneration...
September 13, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28898112/functional-odontoblastic-like-cells-derived-from-human-ipscs
#6
H Xie, N Dubey, W Shim, C J A Ramachandra, K S Min, T Cao, V Rosa
The induced pluripotent stem cells (iPSCs) have an intrinsic capability for indefinite self-renewal and large-scale expansion and can differentiate into all types of cells. Here, we tested the potential of iPSCs from dental pulp stem cells (DPSCs) to differentiate into functional odontoblasts. DPSCs were reprogrammed into iPSCs via electroporation of reprogramming factors OCT-4, SOX2, KLF4, LIN28, and L-MYC. The iPSCs presented overexpression of the reprogramming genes and high protein expressions of alkaline phosphatase, OCT4, and TRA-1-60 in vitro and generated tissues from 3 germ layers in vivo...
September 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28891221/comparative-analysis-of-the-biological-effects-of-the-endodontic-bioactive-cements-mta-angelus-mta-repair-hp-and-neomta-plus-on-human-dental-pulp-stem-cells
#7
C J Tomás-Catalá, M Collado-González, D García-Bernal, R E Oñate-Sánchez, L Forner, C Llena, A Lozano, P Castelo-Baz, J M Moraleda, F J Rodríguez-Lozano
AIM: To evaluate the biological effects in vitro of MTA Angelus (MTA-Ang; Angelus, Londrina, PR, Brazil), MTA Repair HP (MTA-HP; Angelus,) and NeoMTA Plus (NeoMTA-P; Avalon Biomed Inc, Bradenton, FL, USA) on human dental pulp stem cells (hDPSCs). METHODOLOGY: Cell viability and cell migration assays were performed using eluates of each material. To evaluate cell morphology and cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analyzed by immunocytofluorescence and scanning electron microscopy, respectively...
September 11, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28888760/an-in-vitro-investigation-of-indigenous-south-african-medicinal-plants-used-to-treat-oral-infections
#8
S Akhalwaya, S van Vuuren, M Patel
ETHNOPHARMACOLOGICAL RELEVANCE: Over a 120 South African medicinal plants are used for the treatment of oral diseases. Despite the vast collection of antimicrobial studies being done on South African plants, there is still limited research on pathogens associated with oral infections. In consultation with the available ethnobotanical literature, this study investigates the antimicrobial efficacy of some South African medicinal plants against oral pathogens. AIM OF THE STUDY: To provide a detailed account of the antimicrobial properties of selected South African medicinal plants used traditionally to treat oral infections...
September 7, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28887952/evaluating-adhesion-and-alignment-of-dental-pulp-stem-cells-to-a-spider-silk-substrate-for-tissue-engineering-applications
#9
Katherine Hafner, Dallas Montag, Hannah Maeser, Congyue Peng, William R Marcotte, Delphine Dean, Marian S Kennedy
A proposed source of stem cells for nerve regeneration are dental pulp stem cells (DPSCs), based on their close embryonic origin to neurons and the ease with which DPSCs can be obtained from a donor. This study evaluated the response of human DPSCs to spider dragline silk fibers, a potential substrate material for tissue regeneration. The DPSCs' morphology and spread pattern were characterized after these cells were plated onto Nephila clavipes dragline fibers in media. In addition, the responses of two other well established cell lines, osteoblasts (7F2s), and fibroblasts (3T3s), were also studied under identical conditions...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28887537/lngfr-targets-the-wnt-%C3%AE-catenin-pathway-and-promotes-the-osteogenic-differentiation-in-rat-ectomesenchymal-stem-cells
#10
Gang Li, Junyu Liu, Yingying Wang, Kun Yang, Manzhu Zhao, Yong Xiao, Xiujie Wen, Luchuan Liu
Considerable evidence has shown that the Wnt/β-catenin pathway is involved in osteogenic differentiation in various stem cells. However, the role of Wnt/β-catenin pathway in regulating the osteogenic differentiation of rat ectomesenchymal stem cells (EMSCs), which are considered to be the progenitors of dental mesenchymal stem cells, remains unknown. In this study, we demonstrated that nuclear β-catenin was upregulated during EMSC osteogenic differentiation. The Wnt signalling inhibitor IWR-1-endo inhibited EMSC osteogenic differentiation, while the Wnt signalling agonist SKL2001 promoted it...
September 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28887211/gold-nanoparticles-in-injectable-calcium-phosphate-cement-enhance-osteogenic-differentiation-of-human-dental-pulp-stem-cells
#11
Yang Xia, Huimin Chen, Feimin Zhang, Chongyun Bao, Michael D Weir, Mark A Reynolds, Junqing Ma, Ning Gu, Hockin H K Xu
In this study, a novel calcium phosphate cement containing gold nanoparticles (GNP-CPC) was developed. Its osteogenic induction ability on human dental pulp stem cells (hDPSCs) was investigated for the first time. The incorporation of GNPs improved hDPSCs behavior on CPC, including better cell adhesion (about 2-fold increase in cell spreading) and proliferation, and enhanced osteogenic differentiation (about 2-3-fold increase at 14days). GNPs endow CPC with micro-nano-structure, thus improving surface properties for cell adhesion and subsequent behaviors...
September 5, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28886764/advanced-scaffolds-for-dental-pulp-and-periodontal-regeneration
#12
REVIEW
Marco C Bottino, Divya Pankajakshan, Jacques E Nör
No current therapy promotes root canal disinfection and regeneration of the pulp-dentin complex in cases of pulp necrosis. Antibiotic pastes used to eradicate canal infection negatively affect stem cell survival. Three-dimensional easy-to-fit antibiotic-eluting nanofibers, combined with injectable scaffolds, enriched or not with stem cells and/or growth factors, may increase the likelihood of achieving predictable dental pulp regeneration. Periodontitis is an aggressive disease that impairs the integrity of tooth-supporting structures and may lead to tooth loss...
October 2017: Dental Clinics of North America
https://www.readbyqxmd.com/read/28885574/release-of-vegf-from-dental-implant-improves-osteogenetic-process-preliminary-in-vitro-tests
#13
Barbara Zavan, Letizia Ferroni, Chiara Gardin, Stefano Sivolella, Adriano Piattelli, Eitan Mijiritsky
INTRODUCTION: During osseointegration process, the presence of an inflammatory event could negatively influence the proper osteogenetic ability of the implant surface. In order to reduce this possibility, an implementation of angiogenetic event through the release of Vascular Endothelial Growth Factor (VEGF) can be a tool as co-factor for osteoblastic differentiation. In this paper, novel dental implant surfaces enriched with VEGF have been tested. MATERIAL AND METHODS: The ability of VEGF-enriched titanium implants to improve the osteogenetic properties of Mesenchymal stem cells (MSC), also in the presence of an inflammatory environment, have been in vitro tested...
September 8, 2017: Materials
https://www.readbyqxmd.com/read/28883989/osteoinductive-effects-of-preoperative-dexamethasone-in-human-dental-pulp-stem-cells-primary-culture
#14
Rani da Cunha Moretti, Monica Talarico Duailibi, Paulo Oliveira Martins, Jennifer Adriane Dos Santos, Silvio Eduardo Duailibi
AIM: The use of dexamethasone (DEX) in mesenchymal cell culture induces osteoblastic differentiation and, consequently, formation of mineralized tissues. Tissue engineering proposes the development of therapeutic strategies aimed at structural and functional regeneration of biological tissues. In this sense, cell characterization in vitro is critical to ensure the development of such techniques. Our objective was to evaluate the osteoinductive effect of DEX administered as a preoperative medication in primary cell culture of human dental pulp stem cell...
August 2017: Future Science OA
https://www.readbyqxmd.com/read/28879686/development-of-a-bioactive-porous-collagen-%C3%AE-tricalcium-phosphate-bone-graft-assisting-rapid-vascularization-for-bone-tissue-engineering-applications
#15
Nafiseh Baheiraei, Mohamma Reza Nourani, Sm Javad Mortazavi, Mansoureh Movahedin, Hossein Eyni, Fatemeh Baghery, Mohammad Hadi Norahan
We developed collagen (COL) and collagen/beta tricalcium phosphate (COL/β-TCP) scaffolds with a β-TCP/collagen weight ratio of 4 by freeze-drying. Mouse bone marrow-derived mesenchymal stem cells (BMMSCs) were cultured on these scaffolds for 14 days. Samples were characterized by physicochemical analyses and their biological properties such as cell viability and alkaline phosphatase (ALP) activity was,also, examined. Additionally, the vascularization potential of the prepared scaffolds was tested subcutaneously in Wistar rats...
September 6, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28871128/synergistic-effect-of-bdnf-and-fgf2-in-efficient-generation-of-functional-dopaminergic-neurons-from-human-mesenchymal-stem-cells
#16
Manisha Singh, Anupama Kakkar, Rinkey Sharma, O P Kharbanda, Nitika Monga, Manish Kumar, Shantanu Chowdhary, Balram Airan, Sujata Mohanty
To understand the process of neurogenesis, generation of functional dopaminergic (DAergic) neurons from human mesenchymal stem cells (hMSCs) is important. BDNF has been reported to be responsible for inducing neuronal maturation and functionality. Previously, we have reported the efficient generation of neurons from human bone marrow derived MSCs using FGF2 alone. We hypothesize that hMSCs from various tissues [(bone marrow (BM), adipose tissue (AD) and dental pulp (DP)], if treated with BDNF on 9(th) day of induction, alongwith FGF2 will generate functional DAergic neurons...
September 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28866206/differentiation-of-dental-pulp-stem-cells-into-chondrocytes-upon-culture-on-porous-chitosan-xanthan-scaffolds-in-the-presence-of-kartogenin
#17
Cecília B Westin, Rafael B Trinca, Carolina Zuliani, Ibsen B Coimbra, Ângela M Moraes
Adhesion, proliferation and differentiation of dental pulp stem cells (DPSCs) into chondrocytes were investigated in this work with the purpose of broadening the array of cell alternatives to the therapy of cartilage lesions related to tissue engineering approaches. A porous chitosan-xanthan (C-X) matrix was used as scaffold and kartogenin was used as a selective chondrogenic differentiation promoter. The scaffold was characterized regarding aspect and surface morphology, absorption and stability in culture medium, thickness, porosity, thermogravimetric behavior, X-ray diffraction, mechanical properties and indirect cytocompatibility...
November 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28866191/nanosized-cap-silk-fibroin-pcl-peg-pcl-pcl-based-bilayer-membranes-for-guided-bone-regeneration
#18
Sibel Türkkan, A Engin Pazarçeviren, Dilek Keskin, Nesrin E Machin, Özgür Duygulu, Ayşen Tezcaner
Guided bone regeneration (GBR) concept has been developed to prevent the formation of non-functional scar tissue layer on defect site by undertaking barrier role. In this study, a new bilayer membrane which consisted of one layer of electrospun silk fibroin/PCL-PEG-PCL incorporating nanocalcium phosphate (SPCA)(1) and one layer of PCL membrane was developed for GBR. To improve the osteoconductivity of membranes, nanosized calcium phosphate particles synthesized by Flame Spray Pyrolysis method were incorporated into membranes at 10% (wt) (SPCA10) and 20% (wt) (SPCA20) of the polymer content...
November 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28865450/elution-kinetics-of-vancomycin-and-gentamicin-from-carriers-and-their-effects-on-mesenchymal-stem-cell-proliferation-an-in-vitro-study
#19
Tomas Kucera, Lenka Ryskova, Tomas Soukup, Jana Malakova, Eva Cermakova, Pavel Mericka, Jakub Suchanek, Pavel Sponer
BACKGROUND: Musculoskeletal infections remain a major complication in orthopedic surgery. The local delivery of antibiotics provides the high levels required to treat an infection without systemic toxicity. However, the local toxicity of antibiotic carriers to the mesenchymal stem cells, as a result of both the peak concentrations and the type of carrier, may be significant. METHODS: To address this concern, the elution kinetics of vancomycin and gentamicin from several commercially available antibiotic carriers and several carriers impregnated by a surgeon (10 ml of each sterile carrier were manually mixed with a 500 mg vancomycin and an 80 mg gentamicin solution, and the duration of impregnation was 30 min) were assessed...
September 2, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28865173/the-extracts-of-bredigite-bioceramics-enhanced-the-pluripotency-of-human-dental-pulp-cellss
#20
Lihong Chen, Lu Liu, Chengtie Wu, Ruiqi Yang, Jiang Chang, Xi Wei
Biomaterials have a profound effect on tissue engineering and regenerative medicine, but few studies have reported the role of extracts from bioceramics in the regulation of stem cell pluripotency. The present study investigated the effects of bioceramics extracts, including silicate bredigite (Ca7 MgSi4 O16 ) and conventional β-tricalcium phosphate (β-TCP), on the pluripotency and the multilineage differentiation potential of human dental pulp cells (hDPCs). Basic fibroblast growth factor (bFGF), which is a known regulator of hDPCs pluripotency, was used as a reference...
September 2, 2017: Journal of Biomedical Materials Research. Part A
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