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https://www.readbyqxmd.com/read/28812219/eminent-sources-of-adult-mesenchymal-stem-cells-and-their-therapeutic-imminence
#1
REVIEW
Dannie Macrin, Joel P Joseph, Aruthra Arumugam Pillai, Arikketh Devi
In the recent times, stem cell biology has garnered the attention of the scientific fraternity and the general public alike due to the immense therapeutic potential that it holds in the field of regenerative medicine. A breakthrough in this direction came with the isolation of stem cells from human embryo and their differentiation into cell types of all three germ layers. However, the isolation of mesenchymal stem cells from adult tissues proved to be advantageous over embryonic stem cells due to the ethical and immunological naivety...
August 15, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28800076/human-dental-pulp-cells-differentiate-toward-neuronal-cells-and-promote-neuroregeneration-in-adult-organotypic-hippocampal-slices-in-vitro
#2
Li Xiao, Ryoji Ide, Chikako Saiki, Yasuo Kumazawa, Hisashi Okamura
The adult mammalian central nerve system has fundamental difficulties regarding effective neuroregeneration. The aim of this study is to investigate whether human dental pulp cells (DPCs) can promote neuroregeneration by (i) being differentiated toward neuronal cells and/or (ii) stimulating local neurogenesis in the adult hippocampus. Using immunostaining, we demonstrated that adult human dental pulp contains multipotent DPCs, including STRO-1, CD146 and P75-positive stem cells. DPC-formed spheroids were able to differentiate into neuronal, vascular, osteogenic and cartilaginous lineages under osteogenic induction...
August 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28794794/sfrp2-enhances-the-osteogenic-differentiation-of-apical-papilla-stem-cells-by-antagonizing-the-canonical-wnt-pathway
#3
Luyuan Jin, Yu Cao, Guoxia Yu, Jinsong Wang, Xiao Lin, Lihua Ge, Juan Du, Liping Wang, Shu Diao, Xiaomeng Lian, Songlin Wang, Rui Dong, Zhaochen Shan
BACKGROUND: Exploring the molecular mechanisms underlying directed differentiation is helpful in the development of clinical applications of mesenchymal stem cells (MSCs). Our previous study on dental tissue-derived MSCs demonstrated that secreted frizzled-related protein 2 (SFRP2), a Wnt inhibitor, could enhance osteogenic differentiation in stem cells from the apical papilla (SCAPs). However, how SFRP2 promotes osteogenic differentiation of dental tissue-derived MSCs remains unclear...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28793038/transplantation-of-human-immature-dental-pulp-stem-cell-in-dogs-with-chronic-spinal-cord-injury
#4
Matheus Levi Tajra Feitosa, Carlos Alberto Palmeira Sarmento, Renato Zonzini Bocabello, Patrícia Cristina Baleeiro Beltrão-Braga, Graciela Conceição Pignatari, Robson Fortes Giglio, Maria Angelica Miglino, Jéssica Rodrigues Orlandin, Carlos Eduardo Ambrósio
Purpose: To investigate the therapeutic potential of human immature dental pulp stem cells in the treatment of chronic spinal cord injury in dogs. Methods: Three dogs of different breeds with chronic SCI were presented as animal clinical cases. Human immature dental pulp stem cells were injected at three points into the spinal cord, and the animals were evaluated by limb function and magnetic resonance imaging (MRI) pre and post-operative. Results: There was significant improvement from the limb function evaluated by Olby Scale, though it was not supported by the imaging data provided by MRI and clinical sign and evaluation...
July 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28792150/immune-tolerance-of-human-dental-pulp-derived-mesenchymal-stem-cells-mediated-by-cd4%C3%A2-%C2%BAcd25%C3%A2-%C2%BAfoxp3%C3%A2-%C2%BA-regulatory-t-cells-and-induced-by-tgf-%C3%AE-1-and-il-10
#5
Jong Won Hong, Jung Hyun Lim, Chooryung J Chung, Tae Jo Kang, Tae Yeon Kim, Young Seok Kim, Tae Suk Roh, Dae Hyun Lew
PURPOSE: Most studies on immune tolerance of mesenchymal stem cells (MSCs) have been performed using MSCs derived from bone marrow, cord blood, or adipose tissue. MSCs also exist in the craniofacial area, specifically in teeth. The purpose of this study was to evaluate the mechanisms of immune tolerance of dental pulp-derived MSC (DP-MSC) in vitro and in vivo. MATERIALS AND METHODS: We isolated DP-MSCs from human dental pulp and co-cultured them with CD4⁺ T-cells...
September 2017: Yonsei Medical Journal
https://www.readbyqxmd.com/read/28791359/stromal-cell-derived-factor-1-protects-human-periodontal-ligament-stem-cells-against-hydrogen-peroxide-induced-apoptosis
#6
Yimiao Feng, Xiaohui Fu, Xintian Lou, Baiping Fu
Periodontal ligament stem cells (PDLSCs) are considered a promising cell source for dental tissue regeneration. Stromal cell-derived factor 1 [SDF‑1, also known as chemokine (C‑X‑C motif) ligand 12] is regarded as a critical cytokine involved in stem/progenitor cell chemotaxis and homing during tissue regeneration. The present study described a previously unsuspected role for SDF‑1 in the protection of PDLSCs against oxidative stress‑induced apoptosis. In the present study, apoptosis was induced by exposure of PDLSCs to various concentrations of H2O2 for 12 h, following which cell viability was assessed, and cleaved caspase‑3 and ‑9 expression levels were evaluated...
August 8, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28782906/differentiation-of-human-dental-pulp-stem-cells-into-neuronal-by-resveratrol
#7
Ya-Wei Geng, Zhen Zhang, Ming-Yue Liu, Wei-Ping Hu
Dental pulp stem cells (DPSCs) have been proposed as a promising source of stem cells in nerve regeneration due to their close embryonic origin and ease of harvest. Resveratrol (RSV) is a natural polyphenolic and possesses many biological functions such as anti-inflammatory activity and protection against atherosclerosis and neuroprotective activities. There is increasing evidence showing that RSV plays a pivotal role in neuron protection and neuronal differentiation. In this study, we isolated DPSCs from impacted third molars and investigated whether RSV induces neuronal differentiation of DPSCs...
August 7, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28782711/cough-sensors-from-dental-pulp
#8
REVIEW
Lorcan P McGarvey, Rebecca Clarke, Fionnuala T Lundy
Mechanisms which alter sensory neural activity, in particular those rendering nerves hyper-responsive have been implicated in the pathophysiology of common clinical syndromes including chronic cough, itch and pain. However, experimental study of human sensory neurons is challenging because the cell bodies of peripheral neurons are housed in neuronal ganglia which are not accessible using peripheral biopsy techniques. While important advances have been made from studies conducted in animal models, there are interspecies differences...
August 4, 2017: Pulmonary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28781092/properties-of-dental-pulp-derived-mesenchymal-stem-cells-and-the-effects-of-culture-conditions
#9
Nobuyuki Kawashima, Sonoko Noda, Mioko Yamamoto, Takashi Okiji
Dental pulp mesenchymal stem cells (DPMSCs) highly express mesenchymal stem cell markers and possess the potential to differentiate into neural cells, osteoblasts, adipocytes, and chondrocytes. Thus, DPMSCs are considered suitable for tissue regeneration. The colony isolation method has commonly been used to collect relatively large amounts of heterogeneous DPMSCs. Homogenous DPMSCs can be isolated by fluorescence-activated cell sorting using antibodies against mesenchymal stem cell markers, although this method yields a limited number of cells...
August 3, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28781091/dental-pulp-stem-cells-their-potential-in-reinnervation-and-angiogenesis-by-using-scaffolds
#10
Ivo Lambrichts, Ronald B Driesen, Yörg Dillen, Pascal Gervois, Jessica Ratajczak, Tim Vangansewinkel, Esther Wolfs, Annelies Bronckaers, Petra Hilkens
Dental pulp is a highly vascularized and innervated tissue containing a heterogeneous stem cell population with multilineage differentiation potential. Current endodontic treatments focus on the preservation of the pulp tissue and the regeneration of dental pulp after pathological insults. Human dental pulp stem cells (hDPSCs) are currently investigated as stem cell-based therapy for pulp regeneration and for peripheral nerve injury in which neurons and Schwann cells display limited regenerative capacity. We have developed a neuronal differentiation protocol for hDPSCs that requires neurosphere formation before neuronal maturation...
August 3, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28773189/evaluation-of-3d-printed-polycaprolactone-scaffolds-coated-with-freeze-dried-platelet-rich-plasma-for-bone-regeneration
#11
Junda Li, Meilin Chen, Xiaoying Wei, Yishan Hao, Jinming Wang
Three-dimensional printing is one of the most promising techniques for the manufacturing of scaffolds for bone tissue engineering. However, a pure scaffold is limited by its biological properties. Platelet-rich plasma (PRP) has been shown to have the potential to improve the osteogenic effect. In this study, we improved the biological properties of scaffolds by coating 3D-printed polycaprolactone (PCL) scaffolds with freeze-dried and traditionally prepared PRP, and we evaluated these scaffolds through in vitro and in vivo experiments...
July 19, 2017: Materials
https://www.readbyqxmd.com/read/28772088/effect-of-zoledronate-a-third-generation-bisphosphonate-on-proliferation-and-apoptosis-of-human-dental-pulp-stem-cells
#12
Solmaz Pourgonabadi, Seyed Hadi Mousavi, Zahra Tayarani Najaran, Ahmad Ghorbani
Clinical use of zoledronate is accompanied with osteonecrosis of the jaw but the pathogenesis is not well understood. We assumed that zoledronate may have cytotoxicity against stem cells of the oral cavity, and in this way helps to initiate or promoting osteonecrosis. Dental pulp stem cells (DPSCs) and gingival fibroblasts (GFs) were isolated from volunteers who were undergoing third molar extraction. The proliferation of DPSCs and GFs was evaluated using thiazolyl blue tetrazolium bromide assay. Effect of zoledronate on apoptosis was determined by propidium iodide staining and western blotting analysis...
August 3, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28771866/cellular-behaviours-of-bone-marrow-derived-mesenchymal-stem-cells-towards-pristine-graphene-oxide-nanosheets
#13
Changbo Wei, Zifeng Liu, Fangfang Jiang, Binghui Zeng, Mingdi Huang, Dongsheng Yu
OBJECTIVES: Graphene oxide (GO), the derivative of graphene with unique properties, has attracted much attention for applications in dental implants. The aim of this study was, by two biomimetic cell culture methods, to investigate the quantitative relationship between the concentration of pristine GO nanosheets and their cellular behaviours towards bone marrow-derived mesenchymal stem cells (BMSCs). MATERIALS AND METHODS: The cells were firstly characterized according to their morphology, self-renewal capabilities and multipotency...
August 3, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28770470/the-cell-cycle-regulator-protein-p16-and-the-cellular-senescence-of-dental-follicle-cells
#14
Christian Morsczeck, Markus Hullmann, Anja Reck, Torsten E Reichert
Cellular senescence is a restricting factor for regenerative therapies with somatic stem cells. We showed previously that the onset of cellular senescence inhibits the osteogenic differentiation in stem cells of the dental follicle (DFCs), although the mechanism remains elusive. Two different pathways are involved in the induction of the cellular senescence, which are driven either by the cell cycle protein P21 or by the cell cycle protein P16. In this study, we investigated the expression of cell cycle proteins in DFCs after the induction of cellular senescence...
August 2, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28769982/nurr1-downregulation-favors-osteoblastic-differentiation-of-mscs
#15
Adriana Di Benedetto, Francesca Posa, Claudia Carbone, Stefania Cantore, Giacomina Brunetti, Matteo Centonze, Maria Grano, Lorenzo Lo Muzio, Elisabetta A Cavalcanti-Adam, Giorgio Mori
Mesenchymal stem cells (MSCs) have been identified in human dental tissues. Dental pulp stem cells (DPSCs) were classified within MSC family, are multipotent, can be isolated from adult teeth, and have been shown to differentiate, under particular conditions, into various cell types including osteoblasts. In this work, we investigated how the differentiation process of DPSCs toward osteoblasts is controlled. Recent literature data attributed to the nuclear receptor related 1 (NURR1), a still unclarified role in osteoblast differentiation, while NURR1 is primarily involved in dopaminergic neuron differentiation and activity...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28764928/tissue-engineering-dentin-pulp-complex-regeneration-approaches-a-review
#16
REVIEW
Batool Hashemi-Beni, Maryam Khoroushi, Mohammad Reza Foroughi, Saeed Karbasi, Abbas Ali Khademi
Dental pulp is a highly specialized tissue that preserves teeth. It is important to maintain the capabilities of dental pulp before a pulpectomy by creating a local restoration of the dentin-pulp complex from residual dental pulp. The articles identified were selected by two reviewers based on entry and exit criteria. All relevant articles indexed in PubMed, Springer, Science Direct, and Scopus with no limitations from 1961 to 2016 were searched. Factors investigated in the selected articles included the following key words: Dentin-Pulp Complex, Regeneration, Tissue Engineering, Scaffold, Stem Cell, and Growth Factors...
July 25, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28764802/mesenchymal-stem-cells-derived-from-inflamed-dental-pulpal-and-gingival-tissue-a-potential-application-for-bone-formation
#17
Laura Tomasello, Rodolfo Mauceri, Antonina Coppola, Maria Pitrone, Giuseppe Pizzo, Giuseppina Campisi, Giuseppe Pizzolanti, Carla Giordano
BACKGROUND: Chronic periodontal disease is an infectious disease consisting of prolonged inflammation of the supporting tooth tissue and resulting in bone loss. Guided bone regeneration procedures have become common and safe treatments in dentistry, and in this context dental stem cells would represent the ideal solution as autologous cells. In this study, we verified the ability of dental pulp mesenchymal stem cells (DPSCs) and gingival mesenchymal stem cells (GMSCs) harvested from periodontally affected teeth to produce new mineralized bone tissue in vitro, and compared this to cells from healthy teeth...
August 1, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28761445/in-vitro-and-in-vivo-dentinogenic-efficacy-of-human-dental-pulp-derived-cells-induced-by-demineralized-dentin-matrix-and-ha-tcp
#18
Kyung-Jung Kang, Min Suk Lee, Chan-Woong Moon, Jae-Hoon Lee, Hee Seok Yang, Young-Joo Jang
Human dental pulp cells have been known to have the stem cell features such as self-renewal and multipotency. These cells are differentiated into hard tissue by addition of proper cytokines and biomaterials. Hydroxyapatite-tricalcium phosphates (HA-TCPs) are essential components of hard tissue and generally used as a biocompatible material in tissue engineering of bone. Demineralized dentin matrix (DDM) has been reported to increase efficiency of bone induction. We compared the efficiencies of osteogenic differentiation and in vivo bone formation of HA-TCP and DDM on human dental pulp stem cells (hDPSCs)...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28758259/transient-receptor-potential-melastatin-4-channel-is-required-for-rat-dental-pulp-stem-cell-proliferation-and-survival
#19
T D Ngoc Tran, K E Stovall, T Suantawee, Y Hu, S Yao, L-J Yang, S Adisakwattana, H Cheng
OBJECTIVES: Investigate the role of the transient receptor potential melastatin 4 (TRPM4) channel in rat dental pulp stem cell (DPSC) proliferation and survival. MATERIALS AND METHODS: Immunofluorescence and FACS analysis were used to detect the stem cell marker CD90. Alizarin Red S and Oil Red O staining were used to identify osteoblast and adipocyte differentiation, respectively. To characterize TRPM4, patch-clamp recordings were obtained from single cells in the whole-cell configuration mode...
July 30, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28751918/cellular-responses-in-human-dental-pulp-stem-cells-treated-with-three-endodontic-materials
#20
Alejandro Victoria-Escandell, José Santiago Ibañez-Cabellos, Sergio Bañuls-Sánchez de Cutanda, Ester Berenguer-Pascual, Jesús Beltrán-García, Eva García-López, Federico V Pallardó, José Luis García-Giménez, Antonio Pallarés-Sabater, Ignacio Zarzosa-López, Manuel Monterde
Human dental pulp stem cells (HDPSCs) are of special relevance in future regenerative dental therapies. Characterizing cytotoxicity and genotoxicity produced by endodontic materials is required to evaluate the potential for regeneration of injured tissues in future strategies combining regenerative and root canal therapies. This study explores the cytotoxicity and genotoxicity mediated by oxidative stress of three endodontic materials that are widely used on HDPSCs: a mineral trioxide aggregate (MTA-Angelus white), an epoxy resin sealant (AH-Plus cement), and an MTA-based cement sealer (MTA-Fillapex)...
2017: Stem Cells International
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