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Pulp biology

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https://www.readbyqxmd.com/read/28070691/biological-characteristic-effects-of-human-dental-pulp-stem-cells-on-poly-%C3%AE%C2%B5-caprolactone-biphasic-calcium-phosphate-fabricated-scaffolds-using-modified-melt-stretching-and-multilayer-deposition
#1
Natkrita Wongsupa, Thongchai Nuntanaranont, Suttatip Kamolmattayakul, Nuttawut Thuaksuban
Craniofacial bone defects such as alveolar cleft affect the esthetics and functions that need bone reconstruction. The advanced techniques of biomaterials combined with stem cells have been a challenging role for maxillofacial surgeons and scientists. PCL-coated biphasic calcium phosphate (PCL-BCP) scaffolds were created with the modified melt stretching and multilayer deposition (mMSMD) technique and merged with human dental pulp stem cells (hDPSCs) to fulfill the component of tissue engineering for bone substitution...
February 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28067970/fourier-transform-infrared-imaging-analysis-of-dental-pulp-inflammatory-diseases
#2
E Giorgini, S Sabbatini, C Conti, C Rubini, R Rocchetti, M Fioroni, L Memè, G Orilisi
OBJECTIVES: FTIR Microspectroscopy let characterize the macromolecular composition and distribution of tissues and cells, by studying the interaction between infrared radiation and matter. Therefore, we hypothesize to exploit this analytical tool in the analysis of inflamed pulps, to detect the different biochemical features related to various degrees of inflammation. MATERIALS AND METHODS: IR maps of 13 irreversible and 12 hyperplastic pulpitis, together with 10 normal pulps, were acquired, compared with histological findings and submitted to multivariate (HCA, PCA, SIMCA) and statistical (one-way ANOVA) analysis...
January 9, 2017: Oral Diseases
https://www.readbyqxmd.com/read/28067250/promotion-of-natural-tooth-repair-by-small-molecule-gsk3-antagonists
#3
Vitor C M Neves, Rebecca Babb, Dhivya Chandrasekaran, Paul T Sharpe
The restoration of dentine lost in deep caries lesions in teeth is a routine and common treatment that involves the use of inorganic cements based on calcium or silicon-based mineral aggregates. Such cements remain in the tooth and fail to degrade and thus normal mineral volume is never completely restored. Here we describe a novel, biological approach to dentine restoration that stimulates the natural formation of reparative dentine via the mobilisation of resident stem cells in the tooth pulp. Biodegradable, clinically-approved collagen sponges are used to deliver low doses of small molecule glycogen synthase kinase (GSK-3) antagonists that promote the natural processes of reparative dentine formation to completely restore dentine...
January 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28064439/eugenol-specialty-chemical-production-in-transgenic-poplar-populus-tremula-%C3%A3-p-alba-field-trials
#4
Da Lu, Xianghe Yuan, Sung-Jin Kim, Joaquim V Marques, P Pawan Chakravarthy, Syed G A Moinuddin, Randi Luchterhand, Barri Herman, Laurence B Davin, Norman G Lewis
A foundational study assessed effects of biochemical pathway introduction into poplar to produce eugenol, chavicol, p-anol, isoeugenol, and their sequestered storage products, from potentially available substrates, coniferyl and p-coumaryl alcohols. At the onset, it was unknown whether significant carbon flux to monolignols vs other phenylpropanoid (acetate) pathway metabolites would be kinetically favored. Various transgenic poplar lines generated eugenol and chavicol glucosides in ca. 0.45% (~0.35 and ~0...
January 8, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28040177/regulation-of-the-synthesis-of-pulp-degrading-enzymes-in-bacillus-isolated-from-cocoa-fermentation
#5
Honoré G Ouattara, Sylvie Reverchon, Sébastien L Niamke, William Nasser
Pectin degrading enzymes are essential for quality of product from cocoa fermentation. Previously, we studied purified pectate lyases (Pel) produced by Bacillus strains from fermenting cocoa and characterized the cloned pel genes. This study aims to search for biological signals that modulates Pel production and regulators that influence pel gene expression. Strains were grown to the end of exponential phase in media containing various carbon sources. Pel enzymes production in Bacillus was unaffected by simple sugar content variation up to 2%...
May 2017: Food Microbiology
https://www.readbyqxmd.com/read/28033065/effects-of-bioactive-compounds-on-odontogenic-differentiation-and-mineralization
#6
S Phung, C Lee, C Hong, M Song, J K Yi, R G Stevenson, M K Kang, K-H Shin, N-H Park, R H Kim
Direct pulp capping involves the placement of dental materials directly onto vital pulp tissues after deep caries removal to stimulate the regeneration of reparative dentin. This physical barrier will serve as a "biological seal" between these materials and the pulp tissue. Although numerous direct pulp capping materials are available, the use of small bioactive compounds that can potently stimulate and expedite reparative dentin formation is still underexplored. Here, the authors compared and evaluated the pro-osteogenic and pro-odontogenic effects of 4 small bioactive compounds- phenamil (Phen), purmorphamine (Pur), genistein (Gen), and metformin (Met)...
January 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/27986375/biological-evaluation-of-a-new-pulp-capping-material-developed-from-portland-cement
#7
Ahmed M Negm, Ehab E Hassanien, Ashraf M Abu-Seida, Mohamed M Nagy
This study evaluates the biological properties of a new pulp capping material developed from Portland cement. This study was conducted on 48 teeth in 4 dogs (12 teeth/dog). The dogs were classified into two equal groups (n=24 teeth) according to the evaluation period including: group A (3 weeks) and group B (3 months). Each group was further subdivided into three equal subgroups (n=8 teeth) according to the capping material including: subgroup 1: mineral trioxide aggregate (MTA), subgroup2: Portland cement+10% calcium hydroxide+20% bismuth oxide (Port Cal) and subgroup 3: Portland cement+bismuth oxide...
December 13, 2016: Experimental and Toxicologic Pathology: Official Journal of the Gesellschaft Für Toxikologische Pathologie
https://www.readbyqxmd.com/read/27986102/regenerative-endodontic-procedures-a-perspective-from-stem-cell-niche-biology
#8
REVIEW
Manuel Marí-Beffa, Juan José Segura-Egea, Aránzazu Díaz-Cuenca
INTRODUCTION: Endodontics uses cell therapy strategies to treat pulpal and periapical diseases. During these therapies, surgeons aim to reconstruct the natural microenvironments that regulate the activity of dental stem cells. METHODS: We searched for more than 400 articles in PubMed using key words from regenerative endodontics and dental stem cell biology. In 268 articles, we reviewed what factors may influence histologic results after preclinical dental treatments that use regenerative endodontic procedures after pulpectomy...
January 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/27984793/sludge-from-paper-mill-effluent-treatment-as-raw-material-to-produce-carbon-adsorbents-an-alternative-waste-management-strategy
#9
Guilaine Jaria, Carla Patrícia Silva, Catarina I A Ferreira, Marta Otero, Vânia Calisto
Pulp and paper industry produces massive amounts of sludge from wastewater treatment, which constitute an enormous environmental challenge. A possible management option is the conversion of sludge into carbon-based adsorbents to be applied in water remediation. For such utilization it is important to investigate if sludge is a consistent raw material originating reproducible final materials (either over time or from different manufacturing processes), which is the main goal of this work. For that purpose, different primary (PS) and biological sludge (BS) batches from two factories with different operation modes were sampled and subjected to pyrolysis (P materials) and to pyrolysis followed by acid washing (PW materials)...
March 1, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/27982171/does-cryopreservation-affect-the-biological-properties-of-stem-cells-from-dental-tissues-a-systematic-review
#10
Marcus Cristian Muniz Conde, Luiz Alexandre Chisini, Guillermo Grazioli, Alejandro Francia, Rodrigo Varella de Carvalho, Jose Carlos Bernedo Alcázar, Sandra Beatriz Chavez Tarquinio, Flávio Fernando Demarco
This systematic review evaluated if different cryopreservation protocols could affect biological properties (Cell survival rate (CSR), proliferation, differentiation, maintenance of stem cell markers) of stem cells obtained from dental tissues (DSC) post-thaw. An electronic search was carried out within PubMed and ISI Web Science by using specific keyword. Two independent reviewers read the titles and abstracts of all reports respecting predetermined inclusion/exclusion criteria. Data were extracted considering the biological properties of previously cryopreserved DSCs and previously cryopreserved dental tissues...
October 2016: Brazilian Dental Journal
https://www.readbyqxmd.com/read/27979133/isolation-and-characterization-of-a-xylan-with-industrial-and-biomedical-applications-from-edible-a%C3%A3-a%C3%A3-berries-euterpe-oleraceae
#11
Thaisa Moro Cantu-Jungles, Marcello Iacomini, Thales R Cipriani, Lucimara M C Cordeiro
The chemical features of xylan largely determine its physical and biological properties and its use in the industry. In this work, we describe the occurrence, purification and partial characterization of a xylan in edible açaí berries (Euterpe oleraceae), using a fairly simple and inexpensive method of purification from alkaline açaí extract. A mainly linear (1→4)-β-d-xylan was found as the majority (70%) of alkali extract and 4.2% of the dry matter açaí pulp. This represents the biggest source of xylan found so far in a fruit pulp and could be suitable for applications in the industry and biomedical field...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27973697/enhanced-regeneration-potential-of-mobilized-dental-pulp-stem-cells-from-immature-teeth
#12
Hidenori Nakayama, Koichiro Iohara, Yuki Hayashi, Yuta Okuwa, Kurita Kenichi, Misako Nakashima
OBJECTIVES: We have previously demonstrated that dental pulp stem cell (DPSCs) isolated from mature teeth by granulocyte-colony stimulating factor (G-CSF)-induced mobilization method can enhance angiogenesis/vasculogenesis and improve pulp regeneration when compared with colony-derived DPSCs. However, the efficacy of this method in immature teeth with root-formative stage has never been investigated. Therefore, the aim of this study is to examine the stemness, biological characteristics and regeneration potential in mobilized-DPSCs compared with colony-derived DPSCs from immature teeth...
December 14, 2016: Oral Diseases
https://www.readbyqxmd.com/read/27940193/nano-shape-varied-cerium-oxide-nanomaterials-rescue-human-dental-stem-cells-from-oxidative-insult-through-intracellular-or-extracellular-actions
#13
Chinmaya Mahapatra, Rajendra K Singh, Jung-Hwan Lee, Jieun Jung, Jung Keun Hyun, Hae-Won Kim
: Cerium oxide nanomaterials (CeNMs), due to their excellent scavenging properties of reactive oxygen species (ROS), have gained great promise for therapeutic applications. A high level of ROS often degrades the potential of stem cells in terms of survivability, maintenance and lineage differentiation. Here we hypothesize the CeNMs may play an important role in protecting the capacity of stem cells against the oxidative insult, and the suppression mechanism of ROS level may depend on the internalization of CeNMs...
December 9, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27928103/biological-efficacy-of-two-mineral-trioxide-aggregate-mta-based-materials-in-a-canine-model-of-pulpotomy
#14
Myeongyeon Lee, Chung-Min Kang, Je Seon Song, Yooseok Shin, Seunghye Kim, Seong-Oh Kim, Hyung-Jun Choi
The aim of this study was to compare the biocompatibility of Endocem Zr(®) and ProRoot MTA(®) by histopathologic analysis in a canine model of pulpotomy. This study utilized 39 teeth of two beagle dogs. The exposed pulp tissues were treated by pulpotomy using ProRoot MTA (n=19) or Endocem Zr (n=20). After 8 weeks, the teeth were extracted and processed with hematoxylin-eosin staining for histologic evaluation. Most of the specimens in both groups developed a calcific barrier at the pulp amputation site and formed an odontoblast layer...
December 6, 2016: Dental Materials Journal
https://www.readbyqxmd.com/read/27912821/clinical-and-molecular-perspectives-of-reparative-dentin-formation-lessons-learned-from-pulp-capping-materials-and-the-emerging-roles-of-calcium
#15
REVIEW
Minju Song, Bo Yu, Sol Kim, Marc Hayashi, Colby Smith, Suhjin Sohn, Euiseong Kim, James Lim, Richard G Stevenson, Reuben H Kim
The long-term use of calcium hydroxide and the recent increase in the use of hydraulic calcium-silicate cements as direct pulp-capping materials provide important clues in terms of how reparative dentin may be induced to form a "biological seal" to protect the underlying pulp tissues. In this review article, we discuss clinical and molecular perspectives of reparative dentin formation based on evidence learned from the use of these pulp-capping materials. We also discuss the emerging role of calcium as an odontoinductive component in these pulp-capping materials...
January 2017: Dental Clinics of North America
https://www.readbyqxmd.com/read/27912814/biological-molecules-for-the-regeneration-of-the-pulp-dentin-complex
#16
REVIEW
Sahng G Kim
Regenerative endodontic treatment has yielded excellent clinical outcomes, but only several animal studies have shown the robust regeneration of the pulp-dentin complex. The biological molecules, if properly delivered, can enkindle regeneration of dental pulp and dentin rather than repair with tissues of periodontal origin. This review details the biological significance of regenerating the pulp-dentin complex, the effects of biological cues in pulp regeneration, and the delivery strategies of biological molecules to enhance the outcomes of regenerative endodontic therapy...
January 2017: Dental Clinics of North America
https://www.readbyqxmd.com/read/27900666/improved-production-of-thermostable-cellulase-from-thermoascus-aurantiacus-rckk-by-fermentation-bioprocessing-and-its-application-in-the-hydrolysis-of-office-waste-paper-algal-pulp-and-biologically-treated-wheat-straw
#17
Kavish Kumar Jain, Sandeep Kumar, Deepa Deswal, Ramesh Chander Kuhad
Thermostable cellulases have wide variety of applications and distinctive advantages, but their low titer becomes the hurdle in their commercialization. In the present work, an assessment of optimum levels of significant factors (temperature, moisture ratio, inoculum size, and ammonium sulfate) and the effect of their interactions on production of thermostable CMCase, FPase, and β-glucosidase by Thermoascus aurantiacus RCKK under solid-state fermentation (SSF) was carried out using central composite design (CCD) of response surface methodology (RSM)...
November 30, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27898727/biological-markers-for-pulpal-inflammation-a-systematic-review
#18
Dan-Krister Rechenberg, Johnah C Galicia, Ove A Peters
BACKGROUND AND OBJECTIVE: Pulpitis is mainly caused by an opportunistic infection of the pulp space with commensal oral microorganisms. Depending on the state of inflammation, different treatment regimes are currently advocated. Predictable vital pulp therapy depends on accurate determination of the pulpal status that will allow repair to occur. The role of several players of the host response in pulpitis is well documented: cytokines, proteases, inflammatory mediators, growth factors, antimicrobial peptides and others contribute to pulpal defense mechanisms; these factors may serve as biomarkers that indicate the status of the pulp...
2016: PloS One
https://www.readbyqxmd.com/read/27861633/task-allocation-of-wasps-governed-by-common-stomach-a-model-based-on-electric-circuits
#19
Allison Hilbun, Istvan Karsai
Simple regulatory mechanisms based on the idea of the saturable 'common stomach' can control the regulation of construction behavior and colony-level responses to environmental perturbations in Metapolybia wasp societies. We mapped the different task groups to mutual inductance electrical circuits and used Kirchoff's basic voltage laws to build a model that uses master equations from physics, yet is able to provide strong predictions for this complex biological phenomenon. Similar to real colonies, independently of the initial conditions, the system shortly sets into an equilibrium, which provides optimal task allocation for a steady construction, depending on the influx of accessible water...
2016: PloS One
https://www.readbyqxmd.com/read/27857514/the-use-of-bioceramics-in-endodontics-literature-review
#20
REVIEW
Stefan Jitaru, Ioana Hodisan, Lucia Timis, Anamaria Lucian, Marius Bud
BACKGROUND AND AIM: Bioceramics are ceramic compounds obtained both in situ and in vivo, by various chemical processes. Bioceramics exhibit excellent biocompatibility due to their similarity with biological materials, like hydroxyapatite. Bioceramics and multi-substituted hydroxyapatite or similar compounds have the ability to induce a regenerative response in the organism. The aim of this paper is to make a literature review on the main bioceramic materials currently used in endodontics and on their specific characteristics...
2016: Clujul Medical (1957)
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