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Pulp biology

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https://www.readbyqxmd.com/read/29079059/differential-expression-of-long-noncoding-rnas-in-normal-and-inflamed-human-dental-pulp
#1
Xin Huang, Ke Chen
INTRODUCTION: Dental pulp inflammation is an excellent model for the interaction between tissue inflammation and regenerative processes. It is worthwhile to better understand molecular signaling of repair and regeneration in inflammatory processes. Emerging evidence suggests that long noncoding RNA (lncRNA) participates in immune system inflammatory processes. Here we investigate the expression of lncRNAs in pulpitis, the inflammation of dental pulp tissue, and identify lncRNAs that possibly participate in inflammation responses and odontogenesis...
October 24, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/29079052/biocompatibility-of-new-pulp-capping-materials-neomta-plus-mta-repair-hp-and-biodentine-on-human-dental-pulp-stem-cells
#2
Christopher J Tomás-Catalá, Mar Collado-González, David García-Bernal, Ricardo E Oñate-Sánchez, Leopoldo Forner, Carmen Llena, Adrián Lozano, José M Moraleda, Francisco J Rodríguez-Lozano
INTRODUCTION: The aim of the present study was to evaluate the in vitro cytotoxicity of MTA Repair HP, NeoMTA Plus, and Biodentine, new bioactive materials used for dental pulp capping, on human dental pulp stem cells (hDPSCs). METHODS: Biological testing was carried out in vitro on hDPSCs. Cell viability and cell migration assays were performed using eluates of each capping material. To evaluate cell morphology and cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy...
October 24, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/29062364/alcohol-inhibits-odontogenic-differentiation-of-human-dental-pulp-cells-by-activating-mtor-signaling
#3
Wei Qin, Qi-Ting Huang, Michael D Weir, Zhi Song, Ashraf F Fouad, Zheng-Mei Lin, Liang Zhao, Hockin H K Xu
Long-term heavy alcohol consumption could result in a range of health, social, and behavioral problems. People who abuse alcohol are at high risks of seriously having osteopenia, periodontal disease, and compromised oral health. However, the role of ethanol (EtOH) in the biological functions of human dental pulp cells (DPCs) is unknown. Whether EtOH affects the odontoblastic differentiation of DPCs through the mechanistic target of rapamycin (mTOR) remains unexplored. The objective of this study was to investigate the effects of EtOH on DPC differentiation and mineralization...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29054688/collagenous-matrix-supported-by-a-3d-printed-scaffold-for-osteogenic-differentiation-of-dental-pulp-cells
#4
Farahnaz Fahimipour, Erfan Dashtimoghadam, Morteza Rasoulianboroujeni, Mostafa Yazdimamaghani, Kimia Khoshroo, Mohammadreza Tahriri, Amir Yadegari, Jose A Gonzalez, Daryoosh Vashaee, Douglas C Lobner, Tahereh S Jafarzadeh Kashi, Lobat Tayebi
OBJECTIVE: A systematic characterization of hybrid scaffolds, fabricated based on combinatorial additive manufacturing technique and freeze-drying method, is presented as a new platform for osteoblastic differentiation of dental pulp cells (DPCs). METHODS: The scaffolds were consisted of a collagenous matrix embedded in a 3D-printed beta-tricalcium phosphate (β-TCP) as the mineral phase. The developed construct design was intended to achieve mechanical robustness owing to 3D-printed β-TCP scaffold, and biologically active 3D cell culture matrix pertaining to the Collagen extracellular matrix...
October 17, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29054337/dental-pulp-stem-cells-dpscs-increase-prostate-cancer-cell-proliferation-and-migration-under-in-vitro-conditions
#5
Ayşegül Doğan, Selami Demirci, Hüseyin Apdik, Ezgi Avşar Apdik, Fikrettin Şahin
Cancer as a multistep and complicated disease is regulated by several molecular and cellular events. Cancer treatment could be managed at the early stages when the tumor is confined in the tissue. However, disseminated cancer cells metastasize to other body parts and generate new tumors resulting in mortality. Mesenchymal stem cells (MSCs) are found in different body parts and helps adult tissue regeneration. The role of MSCs in cancer progression has emerged as one of the important aspects in cancer biology and is the aim of interest in recent years...
October 16, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/29051649/changes-in-functional-properties-and-in-vitro-bioaccessibilities-of-%C3%AE-carotene-and-lutein-after-extrusion-processing
#6
Melda Ortak, Cagla Caltinoglu, Ilkay Sensoy, Sibel Karakaya, Behic Mert
In this research, carrot pulp was added to traditional snack made from corn-grit. As the biological activity of carotenoids in the body depends on their bioaccessibility, change in carotenoid bioaccessibility during extrusion processing was investigated. In addition, phenolic content, antioxidant activity, β-carotene and lutein contents were investigated before and after the extrusion process. Two different temperature profiles were used for extrusion process. In-vitro bioaccessibilities of β-carotene and lutein increased by extrusion, β-carotene at both temperature profiles while lutein only at higher temperature profile...
October 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28964370/metabolic-fingerprinting-of-must-obtained-from-sun-dried-grapes-of-two-indigenous-cypriot-cultivars-destined-for-the-production-of-commandaria-a-protected-destignation-of-origin-product
#7
Savvas Constantinou, Ana Maria Gómez-Caravaca, Vlasios Goulas, Antonio Segura-Carretero, George A Manganaris
Grapes's sun-dried process is one of the most critical steps in the production of 'Commandaria', a dessert wine product that is exclusively produced in Cyprus and derived from must obtained from two indigenous grape cultivars, namely 'Mavro' and 'Xynisteri'. Despite its significant economic importance, no data regarding the primary and secondary metabolites of the aforementioned cultivars exist. Thus, in the current study, the effect of sun drying process on the qualitative attributes and phenolic profile of 'Mavro' and 'Xynisteri' musts was dissected...
October 2017: Food Research International
https://www.readbyqxmd.com/read/28954668/pulp-and-paper-mill-effluent-management
#8
Ian D Buchanan
This is a review of literature published in 2016 related to the prevention of water pollution by or recovery of beneficial materials from wastewater produced in the pulp and paper industry. This review includes the following sections: pulp and paper wastewater management, pollution avoidance, process modelling, physical and chemical treatment, biological treatment, phytoremediation and value added materials.
October 1, 2017: Water Environment Research: a Research Publication of the Water Environment Federation
https://www.readbyqxmd.com/read/28946303/substantial-equivalence-analysis-in-fruits-from-three-theobroma-species-through-chemical-composition-and-protein-profiling
#9
Walter Pérez-Mora, Jesús V Jorrin-Novo, Luz Marina Melgarejo
Substantial equivalence studies were performed in three Theobroma spp., cacao, bicolor and grandiflorum through chemical composition analysis and protein profiling of fruit (pulp juice and seeds). Principal component analysis of sugar, organic acid, and phenol content in pulp juice revealed equivalence among the three species, with differences in some of the compounds that may result in different organoleptic properties. Proteins were extracted from seeds and pulp juice, resolved by two dimensional electrophoresis and major spots subjected to mass spectrometry analysis and identification...
February 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/28928762/novel-protein-protein-inhibitor-based-approach-to-control-plant-ethylene-responses-synthetic-peptides-for-ripening-control
#10
Mareike Kessenbrock, Simone M Klein, Lena Müller, Mauricio Hunsche, Georg Noga, Georg Groth
Ethylene signaling is decisive for many plant developmental processes. Among these, control of senescence, abscission and fruit ripening are of fundamental relevance for global agriculture. Consequently, detailed knowledge of the signaling network along with the molecular processes of signal perception and transfer are expected to have high impact on future food production and agriculture. Recent advances in ethylene research have demonstrated that signaling of the plant hormone critically depends on the interaction of the ethylene receptor family with the NRAMP-like membrane protein ETHYLENE INSENSITIVE 2 (EIN2) at the ER membrane, phosphorylation-dependent proteolytic processing of ER-localized EIN2 and subsequent translocation of the cleaved EIN2 C-terminal polypeptide (EIN2-CEND) to the nucleus...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28911645/antibiofilm-activity-of-cashew-juice-pulp-against-staphylococcus-aureus-high-performance-liquid-chromatography-diode-array-detection-and-gas-chromatography-mass-spectrometry-analyses-and-interference-on-antimicrobial-drugs
#11
Marcus V Dias-Souza, Renan M Dos Santos, Ezequias P de Siqueira, Pedro H Ferreira-Marçal
The epidemiology of Staphylococcus aureus infections has evolved in recent years, as this species is a major Gram-positive pathogen associated with healthcare services. The antimicrobial resistance of this species raises an urgent need for new treatment strategies. Fruits play important nutritional and economic roles in society, but their biological and pharmacological features are poorly explored when compared to nonedible parts of plants such as barks and leaves. In this study, we show that the cashew apple juice [cashew juice pulp (CJP)] extract is active against the planktonic cells of S...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28899675/kraft-lignin-biorefinery-a-perspective
#12
Jianjun Hu, Quanguo Zhang, Duu-Jong Lee
Lignin is a huge energy and carbon reserve but owing to its highly biologically recalcitrant nature it is commonly regarded as a waste in lignocellulosic biomass biorefinery. To realize the lignin biorefinery, it is proposed to use Kraft lignin, isolated from black liquor from Kraft pulping mills, as starting material to be fragmented by fast pyrolysis or selective catalysis to aromatic sub-units and to be post-refining with additional cleavage reaction and separation/purification as commodity aromatics pool in chemical industries...
September 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28891221/comparative-analysis-of-the-biological-effects-of-the-endodontic-bioactive-cements-mta-angelus-mta-repair-hp-and-neomta-plus-on-human-dental-pulp-stem-cells
#13
C J Tomás-Catalá, M Collado-González, D García-Bernal, R E Oñate-Sánchez, L Forner, C Llena, A Lozano, P Castelo-Baz, J M Moraleda, F J Rodríguez-Lozano
AIM: To evaluate the biological effects in vitro of MTA-Angelus (MTA-Ang; Angelus, Londrina, PR, Brazil), MTA Repair HP (MTA-HP; Angelus) and NeoMTA Plus (NeoMTA-P; Avalon Biomed Inc, Bradenton, FL, USA) on human dental pulp stem cells (hDPSCs). METHODOLOGY: Cell viability and cell migration assays were performed using eluates of each material. To evaluate cell morphology and cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analysed by immunocytofluorescence and scanning electron microscopy, respectively...
September 11, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28887649/metal-concentrations-and-responses-of-chironomid-larvae-exposed-to-thailand-pulp-and-paper-mill-effluent
#14
Natsima Tokhun, Chuleemas Boonthai Iwai, Barry N Noller
Pulp and paper mills (PPM) may discharge insufficiently treated waste into rivers and give rise to serious effects with aquatic life. This study investigated the biological response of the chironomid (Chironomus javanus, Kieffer) when exposed to PPM effluent. Effluent concentrations of BOD, COD, TKN, TS, Cr, Cu, Pb and Zn were high. Cd and Cr concentrations in chironomid were the most accumulated. Whole effluent toxicity on the chironomid test organism was significant as expressed by the percent survival and decreased with increasing concentration...
September 8, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28885570/litchi-chinensis-as-a-functional-food-and-a-source-of-antitumor-compounds-an-overview-and-a-description-of-biochemical-pathways
#15
REVIEW
Sonia Emanuele, Marianna Lauricella, Giuseppe Calvaruso, Antonella D'Anneo, Michela Giuliano
Litchi is a tasty fruit that is commercially grown for food consumption and nutritional benefits in various parts of the world. Due to its biological activities, the fruit is becoming increasingly known and deserves attention not only for its edible part, the pulp, but also for its peel and seed that contain beneficial substances with antioxidant, cancer preventive, antimicrobial, and anti-inflammatory functions. Although literature demonstrates the biological activity of Litchi components in reducing tumor cell viability in in vitro or in vivo models, data about the biochemical mechanisms responsible for these effects are quite fragmentary...
September 8, 2017: Nutrients
https://www.readbyqxmd.com/read/28883989/osteoinductive-effects-of-preoperative-dexamethasone-in-human-dental-pulp-stem-cells-primary-culture
#16
Rani da Cunha Moretti, Monica Talarico Duailibi, Paulo Oliveira Martins, Jennifer Adriane Dos Santos, Silvio Eduardo Duailibi
AIM: The use of dexamethasone (DEX) in mesenchymal cell culture induces osteoblastic differentiation and, consequently, formation of mineralized tissues. Tissue engineering proposes the development of therapeutic strategies aimed at structural and functional regeneration of biological tissues. In this sense, cell characterization in vitro is critical to ensure the development of such techniques. Our objective was to evaluate the osteoinductive effect of DEX administered as a preoperative medication in primary cell culture of human dental pulp stem cell...
August 2017: Future Science OA
https://www.readbyqxmd.com/read/28860516/indirect-immobilized-jagged1-suppresses-cell-cycle-progression-and-induces-odonto-osteogenic-differentiation-in-human-dental-pulp-cells
#17
Jeeranan Manokawinchoke, Praphawi Nattasit, Tanutchaporn Thongngam, Prasit Pavasant, Kevin A Tompkins, Hiroshi Egusa, Thanaphum Osathanon
Notch signaling regulates diverse biological processes in dental pulp tissue. The present study investigated the response of human dental pulp cells (hDPs) to the indirect immobilized Notch ligand Jagged1 in vitro. The indirect immobilized Jagged1 effectively activated Notch signaling in hDPs as confirmed by the upregulation of HES1 and HEY1 expression. Differential gene expression profiling using an RNA sequencing technique revealed that the indirect immobilized Jagged1 upregulated genes were mainly involved in extracellular matrix organization, disease, and signal transduction...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28844305/regenerative-endodontics-for-adult-patients
#18
Ling He, Sahng G Kim, Qimei Gong, Juan Zhong, Sainan Wang, Xuedong Zhou, Ling Ye, Junqi Ling, Jeremy J Mao
The goal of endodontics is to save teeth. Since inception, endodontic treatments are performed to obturate disinfected root canals with inert materials such as gutta-percha. Although teeth can be saved after successful endodontic treatments, they are devitalized and therefore susceptible to reinfections and fractures. The American Association of Endodontists (AAE) has made a tremendous effort to revitalize disinfected immature permanent teeth in children and adolescents with diagnoses including pulp necrosis or apical periodontitis...
September 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28842333/a-theranostic-dental-pulp-capping-agent-with-improved-mri-and-ct-contrast-and-biological-properties
#19
S Mastrogiacomo, N Güvener, W Dou, H S Alghamdi, W A Camargo, J G O Cremers, P J A Borm, A Heerschap, E Oosterwijk, J A Jansen, X F Walboomers
Different materials have been used for vital dental pulp treatment. Preferably a pulp capping agent should show appropriate biological performance, excellent handling properties, and a good imaging contrast. These features can be delivered into a single material through the combination of therapeutic and diagnostic agents (i.e. theranostic). Calcium phosphate based composites (CPCs) are potentially ideal candidate for pulp treatment, although poor imaging contrast and poor dentino-inductive properties are limiting their clinical use...
August 24, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28842267/medicinal-properties-of-mangosteen-garcinia-mangostana-l-a-comprehensive-update
#20
REVIEW
Berenice Ovalle-Magallanes, Dianelena Eugenio-Pérez, José Pedraza-Chaverri
Garcinia mangostana L. (Clusiaceae) is a tropical tree native to Southeast Asia known as mangosteen which fruits possess a distinctive and pleasant taste that has granted them the epithet of "queen of the fruits". The seeds and pericarps of the fruit have a long history of use in the traditional medicinal practices of the region, and beverages containing mangosteen pulp and pericarps are sold worldwide as nutritional supplements. The main phytochemicals present in the species are isoprenylated xanthones, a class of secondary metabolites with multiple reports of biological effects, such as antioxidant, pro-apoptotic, anti-proliferative, antinociceptive, anti-inflammatory, neuroprotective, hypoglycemic and anti-obesity...
August 24, 2017: Food and Chemical Toxicology
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