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Pulp biology

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https://www.readbyqxmd.com/read/28530605/screening-of-passiflora-l-mineral-content-using-principal-component-analysis-and-kohonen-self-organizing-maps
#1
Cleber Galvão Novaes, Ivana Lago da Silva Romão, Berlane Gomes Santos, Jéssica Palhares Ribeiro, Marcos Almeida Bezerra, Erik Galvão Paranhos da Silva
A method using digestion with diluted nitric acid and inductively coupled plasma optical emission spectrometry was applied to determine metal ions in the peel, pulp, and seed of Passiflora edulis f. flavicarpa and Passiflora cincinnata cultivated in the Bahia state, Brazil. Plasma parameters (radio frequency power and argon flow rates) were optimized. The quantification limits (µg g(-1)) were: Ca (43.0); Co (1.3); Cu (10.0); Fe (10.0); K (20.0); Mg (26.6); Mn (0.33); Na (56.6) and Zn (10.0). The accuracy of the method was evaluated by the analysis of certified reference materials (NIST 1567a Wheat Flour and NIST 1577b Bovine Liver)...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28518212/evaluation-of-cytotoxicity-and-ph-changes-generated-by-various-dental-pulp-capping-materials-an-in-vitro-study
#2
Elzbieta Luczaj-Cepowicz, Grazyna Marczuk-Kolada, Malgorzata Pawinska, Marta Obidzinska, Adam Holownia
INTRODUCTION: Various materials are used in direct dental pulp capping method. Their biocompatibility and alkalizing abilities are of primary importance affecting therapeutic effects. The aim of this study was to evaluate and compare the cytotoxicity of various pulp-capping materials on human gingival fibroblasts and investigate the pH changes induced by these materials. MATERIAL AND METHODS: Human gingival fibroblasts were cultured with nine direct pulp materials using culture plate inserts...
May 18, 2017: Folia Histochemica et Cytobiologica
https://www.readbyqxmd.com/read/28491933/wnt-and-bmp-signaling-crosstalk-in-regulating-dental-stem-cells-implications-in-dental-tissue-engineering
#3
Fugui Zhang, Jinglin Song, Hongmei Zhang, Enyi Huang, Dongzhe Song, Viktor Tollemar, Jing Wang, Jinhua Wang, Maryam Mohammed, Qiang Wei, Jiaming Fan, Junyi Liao, Yulong Zou, Feng Liu, Xue Hu, Xiangyang Qu, Liqun Chen, Xinyi Yu, Hue H Luu, Michael J Lee, Tong-Chuan He, Ping Ji
Tooth is a complex hard tissue organ and consists of multiple cell types that are regulated by important signaling pathways such as Wnt and BMP signaling. Serious injuries and/or loss of tooth or periodontal tissues may significantly impact aesthetic appearance, essential oral functions and the quality of life. Regenerative dentistry holds great promise in treating oral/dental disorders. The past decade has witnessed a rapid expansion of our understanding of the biological features of dental stem cells, along with the signaling mechanisms governing stem cell self-renewal and differentiation...
December 2016: Genes & Diseases
https://www.readbyqxmd.com/read/28475038/resolving-stem-and-progenitor-cells-in-the-adult-mouse-incisor-through-gene-co-expression-analysis
#4
Kerstin Seidel, Pauline Marangoni, Cynthia Tang, Bahar Houshmand, Wen Du, Richard L Maas, Steven Murray, Michael C Oldham, Ophir D Klein
Investigations into stem cell-fueled renewal of an organ benefit from an inventory of cell type-specific markers and a deep understanding of the cellular diversity within stem cell niches. Using the adult mouse incisor as a model for a continuously renewing organ, we performed an unbiased analysis of gene co-expression relationships to identify modules of co-expressed genes that represent differentiated cells, transit-amplifying cells, and residents of stem cell niches. Through in vivo lineage tracing, we demonstrated the power of this approach by showing that co-expression module members Lrig1 and Igfbp5 define populations of incisor epithelial and mesenchymal stem cells...
May 5, 2017: ELife
https://www.readbyqxmd.com/read/28456779/effect-of-long-term-culture-on-the-biological-and-morphological-characteristics-of-human-adipose-tissue-derived-stem-cells
#5
L Danisovic, L Oravcova, L Krajciova, Z Varchulova Novakova, M Bohac, I Varga, J Vojtassak
Mesenchymal stem cells (MSCs) are multipotent cells that can be obtained from different tissues, including bone marrow, adipose tissue, umbilical blood, Wharton's jelly, and dental pulp. Due to their differentiation potential, regenerative and immunosuppressive properties, as well as ability to expand under in vitro conditions, these cells represent a promising therapeutic tool for regenerative medicine. However, the basic prerequisite for the therapeutic utilization of MSCs is obtaining a sufficient amount...
February 2017: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/28442657/structural-characterization-and-biological-activities-of-a-novel-polysaccharide-from-phyllanthus-emblica
#6
Zihan Zeng, Wenjie Lv, Yongshuai Jing, Zhiyan Chen, Liyan Song, Ting Liu, Rongmin Yu
A novel water-soluble polysaccharide named PEPW80-1, with a molecular mass of 4.7 kDa, was isolated from the pulp tissues of Phyllanthus emblica, and purified by sephadex G-100 column and sephacryl S-300 HR chromatography. The structural features of PEPW80-1 were investigated by a combination of acid hydrolysis, periodate oxidation-Smith degradation, methylation analysis, gas chromatography-mass spectrometry, scanning electron microscope, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectroscopy...
April 25, 2017: Drug Discoveries & Therapeutics
https://www.readbyqxmd.com/read/28430345/healing-response-of-rat-pulp-treated-with-an-injectable-keratin-hydrogel
#7
Lavanya Ajay Sharma, Robert M Love, Mohammad A Ali, Ajay Sharma, Soraia Macari, Avadhoot Avadhani, George J Dias
BACKGROUND: Keratin has shown promising outcomes as a biomaterial due to its inherent bioactivity, biocompatibility and regenerative effects. The effect of keratin on repair and regeneration of dental tissues has never been studied before. Current therapies to treat pulp tissues involve its replacement with inert, synthetic materials that do not have a proper biological function, leading to failure and tooth loss. This study aimed to develop a biocompatible keratin hydrogel (KH) suitable for pulp therapies...
April 20, 2017: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/28416846/-influence-of-the-arg-gly-asp-ser-sequence-on-the-biological-effects-of-bioactive-glass-on-human-dental-pulp-cells
#8
Y Liu, S N Wang, C Y Cui, Y M Dong
OBJECTIVE: Positive effects of bioactive glass (BG) on proliferation, mineralization, and differentiation of human dental pulp cells (hDPCs) was already verified in various former studies. The Arg-Gly-Asp-Ser sequence (RGDS) was confirmed of affecting cell adhesion. Before further investigation, the objective of this study is to investigate whether RGDS can affect the effects of BG on the adhesion, proliferation and mineralization of hDPCs. METHODS: hDPCs were harvested from third molars of 18-25-year-old individuals after informed consent...
April 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/28398254/fructose-and-pectin-detection-in-fruit-based-food-products-by-surface-enhanced-raman-spectroscopy
#9
Carlo Camerlingo, Marianna Portaccio, Rosarita Tatè, Maria Lepore, Ines Delfino
Surface-Enhanced Raman Spectroscopy (SERS) enables the investigation of samples with weak specific Raman signals, such as opaque samples, including fruit juices and pulp. In this paper, biological apple juices and apple/pear pulp have been studied in order to evidence the presence of fructose and pectin, which are components of great relevance for quality assessment of these kinds of products. In order to perform SERS measurements a low-cost home-made substrate consisting of a glass slide decorated with 30-nm-sized gold nanoparticles has been designed and used...
April 11, 2017: Sensors
https://www.readbyqxmd.com/read/28396925/caffeic-acid-production-by-simultaneous-saccharification-and-fermentation-of-kraft-pulp-using-recombinant-escherichia-coli
#10
Hideo Kawaguchi, Yohei Katsuyama, Du Danyao, Prihardi Kahar, Sachiko Nakamura-Tsuruta, Hiroshi Teramura, Keiko Wakai, Kumiko Yoshihara, Hiromichi Minami, Chiaki Ogino, Yasuo Ohnishi, Ahikiko Kondo
Caffeic acid (3,4-dihydroxycinnamic acid) serves as a building block for thermoplastics and a precursor for biologically active compounds and was recently produced from glucose by microbial fermentation. To produce caffeic acid from inedible cellulose, separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) reactions were compared using kraft pulp as lignocellulosic feedstock. Here, a tyrosine-overproducing Escherichia coli strain was metabolically engineered to produce caffeic acid from glucose by introducing the genes encoding a 4-hydroxyphenyllactate 3-hydroxylase (hpaBC) from Pseudomonas aeruginosa and tyrosine ammonia lyase (fevV) from Streptomyces sp...
April 10, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28389072/the-characteristics-of-mineral-trioxide-aggregate-polycaprolactone-3-dimensional-scaffold-with-osteogenesis-properties-for-tissue-regeneration
#11
Yung-Cheng Chiu, Hsin-Yuan Fang, Tuan-Ti Hsu, Cheng-Yao Lin, Ming-You Shie
INTRODUCTION: The aim of this study was to investigate whether the mineral trioxide aggregate/polycaprolactone (MTA/PCL) hybrid 3-dimensional (3D) scaffold supplies a suitable microenvironment for the osteogenic differentiation of human dental pulp cells (hDPCs) and to further consider the effect of the MTA/PCL composite on the biological performance of hybrid scaffolds. METHODS: MTA was suspended in absolute alcohol and dropped slowly into PCL that was generated with the printable MTA-matrix...
April 4, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28384982/amelogenin-gene-the-pioneer-in-gender-determination-from-forensic-dental-samples
#12
Priyanjali Dutta, Satish Bhosale, Rajeshwar Singh, Priyanka Gubrellay, Jitendra Patil, Bhumika Sehdev, Sachin Bhagat, Tajinder Bansal
INTRODUCTION: In the event of any mass fatality incident, DNA analysis plays a vital role in disaster victim identification. Teeth are one of the most resistant structures in the human body that resist decomposition hence making them prime choice for extracting DNA for identification of individuals. Polymerase Chain Reaction (PCR) analysis that target regions of Amelogenin gene have become the method of choice for sex determination of biological samples. AIM: Determining the sex of a given DNA sample from either dental pulp or dentin of tooth and help in identification of missing persons and disaster victims...
February 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28371765/prerequisite-an-electrohydrolysis-pretreatment-for-anaerobic-digestion-of-lignocellulose-waste-material
#13
C Veluchamy, V Wilson Raju, Ajay S Kalamdhad
This novel work is focused on evaluating the electrohydrolysis pretreatment conditions (applied voltage and time) and anaerobic digestion process for the biological bioconversion of pulp and paper mill sludge into biogas in batch assay. The pretreatment at 15V for 45min shows highest impact on sludge solubilization. The XRD and FT-IR spectroscopic characterization shows the development of aliphatic, unsaturated and carbonyl carbon functionalities in the pretreated samples. FESEM picture also qualities the change in alteration of structure after pretreatment...
March 25, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28351770/2d-nmr-investigation-and-in%C3%A2-vitro-evaluation-of-antioxidant-antigenotoxic-and-estrogenic-antiestrogenic-activities-of-strawberry-grape
#14
Brigida D'Abrosca, Margherita Lavorgna, Monica Scognamiglio, Chiara Russo, Vittoria Graziani, Concetta Piscitelli, Antonio Fiorentino, Marina Isidori
Strawberry grape is considered beneficial due to its extensive phytochemical properties. To expand the knowledge about the chemical constituents and the biological activities of the whole plant, 2D-NMR investigation has been carried out on pulp, peel, seeds, stalks and leaves. Catechin and epicatechin were identified as the main constituents of the seed extract, quercetin and ferulic acid were detected in the leaves and malvidin and cyanidin glucopyranoside in the peels. The leaf, stalk and seed extracts were found to be very rich in phytochemicals and were tested for their ability to reduce the mutagenicity and genotoxicity of standard agents via Salmonella mutagenicity assay and SOS chromotest, respectively...
March 27, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28330085/bioprospecting-and-biotechnological-applications-of-fungal-laccase
#15
REVIEW
Pooja Upadhyay, Rahul Shrivastava, Pavan Kumar Agrawal
Laccase belongs to a small group of enzymes called the blue multicopper oxidases, having the potential ability of oxidation. It belongs to enzymes, which have innate properties of reactive radical production, but its utilization in many fields has been ignored because of its unavailability in the commercial field. There are diverse sources of laccase producing organisms like bacteria, fungi and plants. In fungi, laccase is present in Ascomycetes, Deuteromycetes, Basidiomycetes and is particularly abundant in many white-rot fungi that degrade lignin...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28298928/biological-characteristics-of-fluorescent-superparamagnetic-iron-oxide-labeled-human-dental-pulp-stem-cells
#16
Liang Ma, Ming-Wei Li, Yu Bai, Hui-Hui Guo, Sheng-Chao Wang, Qing Yu
Tracking transplanted stem cells is necessary to clarify cellular properties and improve transplantation success. In this study, we investigate the effects of fluorescent superparamagnetic iron oxide particles (SPIO) (Molday ION Rhodamine-B™, MIRB) on biological properties of human dental pulp stem cells (hDPSCs) and monitor hDPSCs in vitro and in vivo using magnetic resonance imaging (MRI). Morphological analysis showed that intracellular MIRB particles were distributed in the cytoplasm surrounding the nuclei of hDPSCs...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28295583/ameloblastin-upregulates-inflammatory-response-through-induction-of-il-1%C3%AE-in-human-macrophages
#17
Mai Otsuka, Toshinori Okinaga, Wataru Ariyoshi, Chiaki Kitamura, Tatsuji Nishihara
Ameloblastin (AMBN) is an enamel matrix protein that has various biological functions such as healing dental pulp and repairing bone fractures. In the present study, we clarified the effect of AMBN on the expression of an inflammatory cytokine, interleukin-1β (IL-1β) in lipopolysaccharide (LPS)-treated human macrophages. Real-time RT-PCR analysis showed that LPS treatment upregulated expression of the IL-1β gene in U937 cells. Interestingly, AMBN significantly enhanced IL-1β gene expression in LPS-treated U937 cells as well as the secretion of mature IL-1β into culture supernatants by these cells...
March 10, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28294199/management-of-an-immature-partially-necrotic-permanent-molar-by-pulp-revascularization-%C3%A2-two-year-follow-up
#18
Julian Schmoeckel, Mhd Said Mourad, Christian H Splieth, Ruth M Santamaria
Root canal treatment of immature teeth is considered a great challenge for both the dentist and the patient. The thinness of the root canals walls and the lack of an apical barrier leads to unpredictable treatment results. Revascularization is a novel promising treatment option in the field of regenerative endodontics for management of immature necrotic permanent teeth. This case presents a successful treatment of an 8-year-old patient with a partially necrotic, immature permanent mandibular right first molar pulp by revascularization...
March 14, 2017: Quintessence International
https://www.readbyqxmd.com/read/28284102/g-protein-coupled-calcium-sensing-receptor-is-a-crucial-mediator-of-mta-induced-biological-activities
#19
Jin Man Kim, Seulki Choi, Kyu Hwan Kwack, Sun-Young Kim, Hyeon-Woo Lee, Kyungpyo Park
Mineral trioxide aggregate (MTA) is a calcium silicate-based bioactive material that has been extensively used in dentistry. MTA has been highlighted in its diverse biological functions and excellent clinical outcomes. However, limited insight into the intracellular signaling pathways has been provided to explain the biological activities of MTA. Here, we firstly elucidate that the extracellular calcium-sensing receptor (CaSR) is a major signaling mediator of MTA-induced biological reactions through versatile live imaging techniques of human dental pulp cells (hDPCs)...
February 28, 2017: Biomaterials
https://www.readbyqxmd.com/read/28257256/biological-effects-of-provisional-resin-materials-on-human-dental-pulp-stem-cells
#20
S-K Jun, C Mahapatra, H-H Lee, H-W Kim, J-H Lee
OBJECTIVES: This study investigated the in vitro cytotoxicity as well as the proinflammatory cytokine expression of provisional resin materials on primary cultured human dental pulp stem cells (hDPSCs). METHODS: Five commercially available provisional resin materials were chosen (SNAP [SN], Luxatemp [LT], Jet [JE], Revotek LC [RL], and Vipi block [VB]). Eluates that were either polymerizing or already set were added to hDPSCs under serially diluted conditions divided into three different setting times (25% set, 50% set, and 100% set) and incubated for 24 hours with 2× concentrated culture media...
March 2017: Operative Dentistry
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