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Hind limb ischemia

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https://www.readbyqxmd.com/read/28427198/hypoxia-activates-placental-growth-factor-expression-in-lymphatic-endothelial-cells
#1
Laura Tudisco, Augusto Orlandi, Valeria Tarallo, Sandro De Falco
Placental growth factor (PlGF), a proangiogenic member of vascular endothelial growth family, is active during pathological conditions like cancer, metastasis formation and hind limb ischemia and in wound healing. Endothelial cells express PlGF and hypoxia positively modulates in vitro its expression. To verify whether hypoxia modulates PlGF expression in different cellular contexts and in vivo, we first analyzed five human and five mouse cancer cell lines showing that in eight of them hypoxia positively modulates PlGF...
March 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28407473/trimodal-rescue-of-hind-limb-ischemia-with-growth-factors-cells-and-nanocarriers-fundamentals-to-clinical-trials
#2
Rajesh Laksmanan, Gopi Ukani, Muhammad Tipu Rishi, Nilanjana Maulik
Peripheral Artery Disease is a severe medical condition commonly characterized by critical or acute limb ischemia. Gradual accumulation of thrombotic plaques in peripheral arteries of the lower limb may lead to intermittent claudication or ischemia in muscle tissue. Ischemic muscle tissue with lesions may become infected, resulting in a non-healing wound. Stable progression of the non-healing wound associated with severe ischemia might lead to functional deterioration of the limb, which, depending on the severity, can result in amputation...
April 13, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28406931/a-model-of-periventricular-leukomalacia-pvl-in-neonate-mice-with-histopathological-and-neurodevelopmental-outcomes-mimicking-human-pvl-in-neonates
#3
Nahla Zaghloul, Hardik Patel, Mohamed Nagy Ahmed
Periventricular leukomalacia (PVL), a brain injury affecting premature infants is commonly associated with cerebral palsy. PVL results from hypoxia-ischemia (HI) with or without infection and is characterized by white matter necrotic lesions, hypomyelination, microglial activation, astrogliosis, and neuronal death. It is important to study a PVL mouse model that mimics human PVL in symptomatology, anatomic and molecular basis. In our neonate mice model, bilateral carotid arteries were temporary ligated at P5 followed by hypoxic exposure (FiO2 of 8% for 20 min...
2017: PloS One
https://www.readbyqxmd.com/read/28397127/a-novel-strategy-to-enhance-angiogenesis-in-vivo-using-the-small-vegf-binding-peptide-pr1p
#4
Avner Adini, Irit Adini, Zai-Long Chi, Ratmir Derda, Amy E Birsner, Benjamin D Matthews, Robert J D'Amato
Therapeutic angiogenesis is an experimental frontier in vascular biology that seeks to deliver angiogenic growth factors to ischemic or injured tissues to promote targeted formation of new blood vessels as an alternative approach to surgical revascularization procedures. Vascular endothelial growth factor (VEGF) is a potent angiogenic signal protein that is locally upregulated at sites of tissue injury. However, therapies aimed at increasing VEGF levels experimentally by injecting VEGF gene or protein failed to improve outcomes in human trials in part due to its short half-life and systemic toxicity...
April 10, 2017: Angiogenesis
https://www.readbyqxmd.com/read/28392885/the-transcription-factor-bach1-suppresses-the-developmental-angiogenesis-of-zebrafish
#5
Li Jiang, Meng Yin, Jie Xu, Mengping Jia, Shaoyang Sun, Xu Wang, Jianyi Zhang, Dan Meng
Bach1 disrupts Wnt/β-catenin signaling, reduces the proliferation, migration, and tube formation activity of endothelial cells (ECs), and suppresses angiogenesis in mice with surgically induced hind-limb ischemia (HLI). However, the function of Bach1 during developmental angiogenesis in zebrafish remains unclear. Here, we found that zebrafish Bach1 was expressed ubiquitously during early embryonic development in zebrafish. Bach1b mRNA injection of Tg(fli1:gfp) fish disrupted intersegmental vessels (ISV) and dorsal longitudinal anastomotic vessels (DLAV) and suppressed endogenous Wnt/β-catenin signaling and Wnt8a stimulated vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) gene expression at early embryonic stages of zebrafish...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28364777/in-vivo-mri-and-ex-vivo-histological-assessment-of-the-cardioprotection-induced-by-ischemic-preconditioning-postconditioning-and-remote-conditioning-in-a-closed-chest-porcine-model-of-reperfused-acute-myocardial-infarction-importance-of-microvasculature
#6
Tamás Baranyai, Zoltán Giricz, Zoltán V Varga, Gábor Koncsos, Dominika Lukovic, András Makkos, Márta Sárközy, Noémi Pávó, András Jakab, Csilla Czimbalmos, Hajnalka Vágó, Zoltán Ruzsa, Levente Tóth, Rita Garamvölgyi, Béla Merkely, Rainer Schulz, Mariann Gyöngyösi, Péter Ferdinandy
BACKGROUND: Cardioprotective value of ischemic post- (IPostC), remote (RIC) conditioning in acute myocardial infarction (AMI) is unclear in clinical trials. To evaluate cardioprotection, most translational animal studies and clinical trials utilize necrotic tissue referred to the area at risk (AAR) by magnetic resonance imaging (MRI). However, determination of AAR by MRI' may not be accurate, since MRI-indices of microvascular damage, i.e., myocardial edema and microvascular obstruction (MVO), may be affected by cardioprotection independently from myocardial necrosis...
April 1, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28351415/role-of-carbonyl-sulfide-in-acute-lung-injury-following-limb-ischemia-reperfusion-in-rats
#7
Yan-Rui Zhao, Wen-Rui Lv, Jun-Lin Zhou
OBJECTIVE: To investigate the effect of carbonyl sulfide (COS) on limb ischemia/reperfusion (I/R)-induced acute lung injury (ALI) and the associated mechanism in rats. METHODS: ALI was induced by bilateral hind limb I/R in Sprague-Dawley (SD) rats. Sixty-four SD rats were randomly divided into the control group, I/R group, I/R + COS group, and I/R + AIR group. We observed the survival rate of the rats and the morphological changes of lung tissues, and we measured the change in the lung coefficient, the expression levels of the intercellular adhesion factor-1 (ICAM-1) protein in lung tissue, the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-lβ, and interleukin (IL)-6 in both lung tissue and serum, and cell apoptosis...
March 28, 2017: European Journal of Medical Research
https://www.readbyqxmd.com/read/28347862/cell-sheet-engineering-using-the-stromal-vascular-fraction-of-adipose-tissue-as-a-vascularization-strategy
#8
Marina Costa, Mariana T Cerqueira, Tírcia C Santos, Belém Sampaio-Marques, Paula Ludovico, Alexandra P Marques, Rogério P Pirraco, Rui L Reis
Current vascularization strategies for Tissue Engineering constructs, in particular cell sheet-based, are limited by time-consuming and expensive endothelial cell isolation and/or by the complexity of using extrinsic growth factors. Herein, we propose an alternative strategy using angiogenic cell sheets (CS) obtained from the stromal vascular fraction (SVF) of adipose tissue that can be incorporated into more complex constructs. Cells from the SVF were cultured in normoxic and hypoxic conditions for up to 8 days in the absence of extrinsic growth factors...
March 24, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28302707/neuropeptide-y3-36-incorporated-into-pvax-nanoparticle-improves-functional-blood-flow-in-a-murine-model-of-hind-limb-ischemia
#9
Derek Eshun, Rabya Saraf, Soochan Bae, Jelliffe Jeganathan, Feroze Mahmood, Serkan Dilmen, Qingen Ke, Dongwon Lee, Peter M Kang, Robina Matyal
We generated a novel nanoparticle called PVAX, which has intrinsic anti-apoptotic and anti-inflammatory properties. This nanoparticle was loaded with Neuropeptide Y3-36, an angiogenic neuro-hormone that plays a central role in angiogenesis. Subsequently, we investigated whether PVAX-NPY3-36 could act as a therapeutic agent and induce angiogenesis and vascular remodeling in a murine model of hind limb ischemia. Adult C57BL/J6 mice (n=40) were assigned to treatment groups: Control, Ischemia PBS, Ischemia PVAX, Ischemia NPY3-36, and Ischemia PVAX-NPY3-36 Ischemia was induced by ligation of the femoral artery in all groups except Control and given relevant treatments (PBS, PVAX, NPY3-36, and PVAX-NPY3-36)...
March 16, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28287126/pet-cell-tracking-using-18-f-flt-is-not-limited-by-local-reuptake-of-free-radiotracer
#10
Mark G MacAskill, Adriana S Tavares, Junxi Wu, Christophe Lucatelli, Joanne C Mountford, Andrew H Baker, David E Newby, Patrick W F Hadoke
Assessing the retention of cell therapies following implantation is vital and often achieved by labelling cells with 2'-[(18)F]-fluoro-2'-deoxy-D-glucose ((18)F-FDG). However, this approach is limited by local retention of cell-effluxed radiotracer. Here, in a preclinical model of critical limb ischemia, we assessed a novel method of cell tracking using 3'-deoxy-3'-L-[(18)F]-fluorothymidine ((18)F-FLT); a clinically available radiotracer which we hypothesise will result in minimal local radiotracer reuptake and allow a more accurate estimation of cell retention...
March 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28266625/inhibition-of-phd3-by-salidroside-promotes-neovascularization-through-cell-cell-communications-mediated-by-muscle-secreted-angiogenic-factors
#11
Jing Zhang, Vivi Kasim, Yu-Dan Xie, Can Huang, Julita Sisjayawan, Agnes Dwi Ariyanti, Xue-Song Yan, Xiao-Yan Wu, Cai-Ping Liu, Li Yang, Makoto Miyagishi, Shou-Rong Wu
Therapeutic angiogenesis has been considered as a potential strategy for treating peripheral artery diseases including hind-limb ischemia (HLI); however, no effective drug-based treatment is currently available. Here we showed that intramuscular administration of salidroside, an active compound of Chinese herb Rhodiola, could robustly enhance blood perfusion recovery by promoting neovascularization in HLI mice. We revealed that salidroside promoted skeletal muscle cell migration and paracrine function through inhibiting the transcriptional level of prolyl-hydroxylase domain 3 (PHD3) without affecting PHD1 and PHD2...
March 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28261920/remote-physiological-ischemic-training-promotes-coronary-angiogenesis-via-molecular-and-cellular-mobilization-after-myocardial-ischemia
#12
Yu Zheng, Mingyue Xiao, Ling Li, Jianan Li, Jan D Reinhardt, Xiao Lu
AIM: This study was designed to explore the mechanism of physiological ischemic training (PIT) on remote limbs, with a special focus on the potential role of endothelial progenitor cells (EPCs), vascular endothelial growth factor (VEGF) and nitric oxide (NO) in local ischemic myocardium. METHOD: MI rabbit models were randomly assigned to sham-operated group (SO), training only group (TO), myocardial ischemia group (MI), PIT group, VEGF inhibitor group (VEGF-), NO inhibitor group (NO-), VEGF and NO inhibitor group (VEGF-/NO-), and EPC inhibitor group (EPC-)...
March 6, 2017: Cardiovascular Therapeutics
https://www.readbyqxmd.com/read/28258850/angiogenic-characteristics-of-human-stromal-vascular-fraction-in-ischemic-hindlimb
#13
Enze Jin, Dong-Sik Chae, Mina Son, Sung-Whan Kim
INTRODUCTION: In this study, we sought to characterize the angio-vasculogenic property of human adipose tissue-derived stromal vascular fraction (SVF) and to determine the therapeutic potential of SVF in the context of experimental ischemia. Although human SVF is used for cell therapy, its angiogenic and vasculogenic characteristics have not been fully elucidated. METHODS AND RESULTS: We conducted flow cytometry, microarray, quantitative (q)-PCR, Matrigel tube formation assays and in vivo therapeutic assays using an ischemic hind limb mouse model...
May 1, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28216514/diallyl-trisulfide-augments-ischemia-induced-angiogenesis-via-an-endothelial-nitric-oxide-synthase-dependent-mechanism
#14
Ryo Hayashida, Kazuhisa Kondo, Sumio Morita, Kazumasa Unno, Satoshi Shintani, Yuuki Shimizu, John W Calvert, Rei Shibata, Toyoaki Murohara
BACKGROUND: Hydrogen sulfide (H2S) exerts beneficial actions against the development of cardiovascular disease. Diallyl trisulfide (DATS) is an organic polysulfide found in garlic oil that liberates H2S under physiological conditions. This study investigated whether DATS modulates endothelial cell function, as well as revascularization processes in a mouse model of hind-limb ischemia.Methods and Results:Wild-type (WT), endothelial nitric oxide synthase-deficient (eNOS-KO) and Akt1-heterogenic deficient (Akt-Het) mice were subjected to unilateral hindlimb ischemia (HLI)...
February 17, 2017: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/28216347/systemic-application-of-carbon-monoxide-releasing-molecule-3-protects-skeletal-muscle-from-ischemia-reperfusion-injury
#15
Aurelia Bihari, Gediminas Cepinskas, Thomas L Forbes, Richard F Potter, Abdel-Rahman Lawendy
OBJECTIVE: Ischemia-reperfusion (IR) is a limb- and life-threatening complication of acute limb ischemia and musculoskeletal trauma. Carbon monoxide-releasing molecules (CORMs) have recently been shown to protect microvascular perfusion and to reduce inflammation and injury in various ischemic animal models. The purpose of this study was to examine the effects of water-soluble CORM-3 on the extent of IR-induced muscle injury. METHODS: Wistar rats were randomized into three groups: sham (no ischemia), IR + CORM-3 (10 mg/kg intraperitoneally), and IR + inactive CORM-3 (iCORM-3; 10 mg/kg intraperitoneally)...
February 16, 2017: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/28210050/a-study-of-the-relationships-of-changes-in-pain-and-gait-after-tourniquet-induced-ischemia-reperfusion-in-rats
#16
Kazuki Aihara, Takeya Ono, Namiko Umei, Wakako Tsumiyama, Atsushi Tasaka, Hideki Ishikura, Yuta Sato, Tomohiro Matsumoto, Sadaaki Oki
[Purpose] The purpose of this study was to determine the relationships of changes in pain and gait after ischemia reperfusion was induced by tourniquet in rats. [Subjects and Methods] The subjects were six ten-week-old male Wistar rats. Ischemia was induced in the left lower limbs of the experimental rats at a pressure of 300 mmHg for 90 minutes. Pain behavior evaluations were measured using the von Frey test in all the rats' hind limbs. A consistently increasing plantar stimulus was applied until the rats exhibited an escape behavior...
January 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28207177/quaking-is-a-key-regulator-of-endothelial-cell-differentiation-neovascularization-and-angiogenesis
#17
Amy Cochrane, Sophia Kelaini, Marianna Tsifaki, James Bojdo, Marta Vilà-González, Daiana Drehmer, Rachel Caines, Corey Magee, Magdalini Eleftheriadou, Yanhua Hu, David Grieve, Alan W Stitt, Lingfang Zeng, Qingbo Xu, Andriana Margariti
The capability to derive endothelial cell (ECs) from induced pluripotent stem cells (iPSCs) holds huge therapeutic potential for cardiovascular disease. This study elucidates the precise role of the RNA-binding protein Quaking isoform 5 (QKI-5) during EC differentiation from both mouse and human iPSCs (hiPSCs) and dissects how RNA-binding proteins can improve differentiation efficiency toward cell therapy for important vascular diseases. iPSCs represent an attractive cellular approach for regenerative medicine today as they can be used to generate patient-specific therapeutic cells toward autologous cell therapy...
April 2017: Stem Cells
https://www.readbyqxmd.com/read/28194544/exploring-the-role-of-trpv-and-cgrp-in-adenosine-preconditioning-and-remote-hind-limb-preconditioning-induced-cardioprotection-in-rats
#18
Amritpal Singh, Puneet Kaur Randhawa, Anjana Bali, Nirmal Singh, Amteshwar Singh Jaggi
The cardioprotective effects of remote hind limb preconditioning (RIPC) are well known, but mechanisms by which protection occurs still remain to be explored. Therefore, the present study was designed to investigate the role of TRPV and CGRP in adenosine and remote preconditioning-induced cardioprotection, using sumatriptan, a CGRP release inhibitor and ruthenium red, a TRPV inhibitor, in rats. For remote preconditioning, a pressure cuff was tied around the hind limb of the rat and was inflated with air up to 150 mmHg to produce ischemia in the hind limb and during reperfusion pressure was released...
February 14, 2017: Cardiovascular Drugs and Therapy
https://www.readbyqxmd.com/read/28188848/electroacupuncture-preconditioning-and-postconditioning-inhibit-apoptosis-and-neuroinflammation-induced-by-spinal-cord-ischemia-reperfusion-injury-through-enhancing-autophagy-in-rats
#19
Bo Fang, Meiman Qin, Yun Li, Xiaoqian Li, Wenfei Tan, Ying Zhang, Hong Ma
Electroacupuncture (EA) has beneficial effects on spinal cord ischemia reperfusion (I/R) injury, but the underlying mechanisms are not fully understood. This study aimed to investigate the role of autophagy in the protection of EA preconditioning and postconditioning against spinal cord I/R injury. For this, spinal cord I/R injury was induced by 14min occlusion of the aortic arch, and rats were treated with EA for 20min before or after the surgery. The expression of autophagy components, light chain 3 and Beclin 1, was assessed by Western blot...
March 6, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28167285/nanoparticular-delivery-system-for-a-secretoneurin-derivative-induces-angiogenesis-in-a-hind-limb-ischemia-model
#20
Karin Albrecht-Schgoer, Jan Barthelmes, Wilfried Schgoer, Markus Theurl, Isabelle Nardin, Daniela Lener, Clemens Gutmann, Sarah Dünnhaupt, Andreas Bernkop-Schnürch, Rudolf Kirchmair
Common therapeutic strategies for peripheral arterial disease often fail to re-establish sufficient blood flow within legs and feet of patients for avoiding critical limb ischemia, what is characterized by a substantial risk for amputation. The neuropeptide secretoneurin induces angiogenesis in models of limb and myocardial ischemia and might be a promising tool in the treatment of patients without the option of revascularization therapy for severe ischemia. Within this manuscript, the biologically active part of secretoneurin was identified, modified by induction of a cysteine residue to gain higher stability against enzymatic degradation and further packed into S-protected thiolated chitosan nanoparticles, which enable intra-muscular application of secretoneurin...
February 3, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
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