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https://www.readbyqxmd.com/read/28109025/immune-regulation-by-t-regulatory-cells-in-hbv-related-inflammation-and-cancer
#1
REVIEW
Nirupama Trehanpati, Ashish Kumar Vyas
Hepatocellular carcinoma (HCC) is the leading cause of cancer death and hepatitis B virus (HBV) infection is one of the commonest causes in Asians countries. India has the second largest pool after China for hepatitis B infected subjects. HBV clearance is T cell dependent and one of the reason for T cells hypo responsiveness is due to mass production of Tregs through activation of Notch signaling, which suppressCD4/CD8 T cells. Regulatory T cells (Tregs) are important to maintain cellular homeostasis; however during viral infection increase of Tregs is inversely proportional to HBV DNA titers...
January 21, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28108961/effects-of-negatively-and-positively-charged-ti-metal-surfaces-on-ceramic-coating-adhesion-and-cell-response
#2
Rodney Marcelo do Nascimento, Vanessa Rafaela de Carvalho, José Silvio Govone, Antônio Carlos Hernandes, Nilson Cristino da Cruz
This manuscript reports an evaluation of the effects of simple chemical-heat treatments on the deposition of different ceramic coatings, i.e., TiO2, CaTiO3 and CaP, on commercially pure titanium (cp-Ti) and Ti6Al4V and the influence of the coatings on cells interaction with the surfaces. The ceramic materials were prepared by the sol-gel method and the coating adhesion was analyzed by pull-off bending tests. The wettability of positively or negatively charged surfaces was characterized by contact angle measurements, which also enabled the calculation of the surface free energy through the polar-apolar liquids approach...
February 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28106783/extract-of-polygala-tenuifolia-alleviates-stress-exacerbated-atopy-like-skin-dermatitis-through-the-modulation-of-protein-kinase-a-and-p38-mitogen-activated-protein-kinase-signaling-pathway
#3
Bongjun Sur, Bombi Lee, Ye Seul Yoon, Pooreum Lim, Riwon Hong, Mijung Yeom, Hyang Sook Lee, Hijoon Park, Insop Shim, Hyejung Lee, Young Pyo Jang, Dae-Hyun Hahm
Atopic dermatitis (AD) and stress create a vicious cycle: stress exacerbates atopic symptoms, and atopic disease elicits stress and anxiety. Targeting multiple pathways including stress and allergic inflammation is, therefore, important for treating AD. In this study, we investigated the remedial value of Polygala tenuifolia Willd. (PTW) for treating immobilization (IMO) stress-exacerbated atopy-like skin dermatitis and its underlying mechanism. Trimellitic anhydride (TMA) was applied to dorsal skin for sensitization and subsequently both ears for eliciting T-cell-dependent contact hypersensitivity in mice, which underwent 2 h-IMO stress and PTW administration for the latter 6 and 9 days in the ear exposure period of TMA, respectively...
January 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28105982/human-umbilical-cord-mesenchymal-stem-cells-inhibit-t-follicular-helper-cell-expansion-through-the-activation-of-inos-in-lupus-prone-b6-mrl-faslpr-mice
#4
Zhuoya Zhang, Ruihai Feng, Lingying Niu, Saisai Huang, Wei Deng, Bingyu Shi, Genhong Yao, Weiwei Chen, Xiaojun Tang, Xiang Gao, Xuebing Feng, Lingyun Sun
The aberrant generation or activation of T follicular helper (Tfh) cells contributes to the pathogenesis of systemic lupus erythematosus (SLE), yet little is known about how these cells are regulated. In this study, we demonstrated that the frequency of Tfh cells was increased in lupus-prone B6.MRL-<em>Fas<sup>lpr</sup></em> (B6.<em>lpr</em>) mice and positively correlated to plasma cell proportions and serum total IgG as well as anti-dsDNA antibody levels. Transplantation of human umbilical cord mesenchymal stem cells (UC-MSCs) ameliorated lupus symptoms in B6...
January 20, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28105269/electrophilic-helical-peptides-that-bond-covalently-irreversibly-and-selectively-in-a-protein-protein-interaction-site
#5
Aline Dantas de Araujo, Junxian Lim, Andrew C Good, Renato T Skerlj, David P Fairlie
Protein-protein interactions mediate most physiological and disease processes. Helix-constrained peptides potently mimic or inhibit these interactions by making multiple contacts over large surface areas. However, despite high affinities, they typically have short lifetimes bound to the protein. Here we insert both a helix-inducing constraint and an adjacent electrophile into the native peptide ligand BIM to target the oncogenic protein Bcl2A1. The modified BIM peptide bonds covalently and irreversibly to one cysteine within the helix-binding groove of Bcl2A1, but not to two other exposed cysteines on its surface, and shows no covalent bonding to other Bcl2 proteins...
January 12, 2017: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28104899/amino-acid-based-material-for-the-complementary-therapy-of-decubitus-ulcers
#6
Frederico Nogueira, Isabel C Gouveia
Chronic wounds, pressure sores, lesions and infections of microbial origin in bedridden, paralyzed or malnutrition patients remain the object of study of many researchers. There are several intrinsic and extrinsic factors involved in the development of these disorders, which are related to the patient's immune system, making it unable to respond effectively to the treatment of the wound. These factors can be properly controlled, giving particular importance to the ethiology and stage of the wound, as well as the time periods corresponding to the replacement of the dressings...
January 20, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28104015/-establishment-and-characterization-of-a-laryngeal-squamous-cell-carcinoma-cell-line
#7
R Wang, X C Bian, Y Q Liu, J G Fang, H Wang, H G Liu, M Lian, H Z Ma, L Feng, H Z Wang
Objective: To establish a laryngeal squamous cell carcinoma (LSCC) cell line through primary cell culture and observe its biological characteristics. Methods: Tissue block culture method was used for primary cell culture. After LSCC cells passed 25 times in vitro, the morphology of cells was observed, keratin was stained histochemically, cell cycle was tested by PI-FACS, and the specie of cells was detected by PCR and short tandem repeat(STR) typing. Results: This newly established LSCC cell line was named as TR-LCC-1, most of the cancer cells were polygonal shape, like the cobblestone, loss of contact inhibition and with overlapping growth...
January 7, 2017: Zhonghua Er Bi Yan Hou Tou Jing Wai Ke za Zhi, Chinese Journal of Otorhinolaryngology Head and Neck Surgery
https://www.readbyqxmd.com/read/28103785/non-peptide-crf-receptor-antagonists-allosterism-kinetics-and-translation-to-efficacy-in-human-disease
#8
Dimitri E Grigoriadis, Samuel R J Hoare
G-Protein coupled receptors (GPCRs) have been, and remain a key target of drug discovery programs for human disease. While many drugs have been developed that interact with these proteins in the simple classic manner - that is - physically blocking the cognate ligand from simply binding to its target receptor, drug discovery approaches have elucidated alternative more complex methods by which small molecules can interact with these receptors and block their function. This is most evident in the Class B GPCRs where the cognate ligands are relatively large peptides with multiple points of contact on the GPCR spanning both hydrophilic and hydrophobic domains on the same protein to elicit function...
January 10, 2017: Current Molecular Pharmacology
https://www.readbyqxmd.com/read/28101211/receptor-fgfrl1-acts-as-a-tumor-suppressor-in-nude-mice-when-overexpressed-in-hek-293-tet-on-cells
#9
Lei Zhuang, Florian Steinberg, Beat Trueb
Fibroblast growth factor receptor-like 1 (FGFRL1) is a transmembrane receptor that interacts with heparin and FGF ligands. In contrast to the classical FGF receptors, FGFR1 to FGFR4, it does not appear to affect cell growth and proliferation. In the present study, an inducible gene expression system was utilized in combination with a xenograft tumor model to investigate the effects of FGFRL1 on cell adhesion and tumor formation. It was determined that recombinant FGFRL1 promotes the adhesion of HEK 293 Tet-On(®) cells in vitro...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28100054/efficacy-of-silver-hydrophilic-poly-p-xylylene-on-preventing-bacterial-growth-and-biofilm-formation-in-urinary-catheters
#10
Hamideh Heidari Zare, Viktorija Juhart, Attila Vass, Gerhard Franz, Dieter Jocham
Catheter associated urinary tract infections (CAUTI), caused by several strains of bacteria, are a common complication for catheterized patients. This may eventually lead to a blockage of the catheter due to the formation of a crystalline or amorphous biofilm. Inhibiting bacteria should result in a longer application time free of complaints. This issue has been investigated using an innovative type of silver-coated catheter with a semipermeable cap layer to prevent CAUTI. In this work, two different types of silver catheters were investigated, both of which were capped with poly(p-xylylene) (PPX-N) and exhibited different surface properties that completely changed their wetting conduct with water...
January 18, 2017: Biointerphases
https://www.readbyqxmd.com/read/28099143/desirable-cytolytic-immune-effector-cell-recruitment-by-interleukin-15-dendritic-cells
#11
Heleen H Van Acker, Ottavio Beretta, Sébastien Anguille, Lien De Caluwé, Angela Papagna, Johan M Van den Bergh, Yannick Willemen, Herman Goossens, Zwi N Berneman, Viggo F Van Tendeloo, Evelien L Smits, Maria Foti, Eva Lion
Success of dendritic cell (DC) therapy in treating malignancies is depending on the DC capacity to attract immune effector cells, considering their reciprocal crosstalk is partially regulated by cell-contact-dependent mechanisms. Although critical for therapeutic efficacy, immune cell recruitment is a largely overlooked aspect regarding optimization of DC vaccination. In this paper we have made a head-to-head comparison of interleukin (IL)-15-cultured DCs and conventional IL-4-cultured DCs with regard to their proficiency in the recruitment of (innate) immune effector cells...
January 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/28099027/inactivation-of-soybean-trypsin-inhibitor-by-epigallocatechin-gallate-stopped-flow-fluorescence-thermodynamics-and-docking-studies
#12
Chun Liu, Fenfen Cheng, Xiaoquan Yang
Tea is one of the most widely daily consumed beverages all over the world, and it is usually consumed with milk and/or soy milk. However, very few researches have studied the interactions between tea polyphenols (TPs) and soy milk proteins as compared with milk proteins. Here, we reported that epigallocatechin gallate (EGCG), a major component of TPs, can effectively inhibit the inhibitory activity of Kunitz trypsin inhibitor (KTI, a major antinutrient in soy milk). The mechanism of inactivation of KTI by EGCG was investigated by stopped-flow/fluorescence, thermodynamics, and docking studies...
January 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28098447/the-stories-tryptophans-tell-exploring-protein-dynamics-of-heptosyltransferase-i-from-escherichia-coli
#13
Joy M Cote, Carlos Andres Ramirez-Mondragon, Zarek S Siegel, Daniel Joseph Czyzyk, Jiali Gao, Yuk Yin Sham, Ishita Mukerji, Erika A Taylor
Heptosyltransferase I (HepI) catalyzes the addition of a single L-glycero-β-D-manno-heptose sugar (heptose) onto the membrane incorporated Kdo2-Lipid A as part of the biosynthesis of the core region of lipopolysaccharide (LPS). Gram-negative bacteria with gene knockouts of HepI have reduced virulence with enhanced susceptibility to hydrophobic antibiotics, making design of inhibitors against HepI of interest. Previous studies have shown that HepI protein dynamics are partially rate limiting, and therefore disruption of protein dynamics might provide a new strategy for inhibiting HepI...
January 18, 2017: Biochemistry
https://www.readbyqxmd.com/read/28096300/enhanced-suppression-of-polyclonal-cd8-25-regulatory-t-cells-via-exosomal-arming-of-antigen-specific-peptide-mhc-complexes
#14
Chuanyong Mu, Xueshu Zhang, Lu Wang, Aizhang Xu, Khawaja Ashfaque Ahmed, Xueqin Pang, Rajni Chibbar, Andrew Freywald, Jianan Huang, Yehan Zhu, Jim Xiang
Compared with CD4(+)25(+) regulatory T cells (Tregs), the mechanisms for natural, polyclonal CD8(+)25(+) Treg immune suppression have been significantly less studied. We previously showed that polyclonal T cells can acquire antigen-specific targeting activity through arming with exosomal peptide-MHC (pMHC). In this study, we assessed the suppressive effect of CD8(+)25(+) Tregs or CD8(+)25(+) Tregs armed with ovalbumin (OVA)-specific exosomes on other immune cells and OVA-specific dendritic cell (DCOVA)-stimulated antitumor immunity...
January 17, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28093367/effect-of-construction-of-tio2-nanotubes-on-platelet-behaviors-structure-property-relationships
#15
Qiaoling Huang, Yun Yang, Dajiang Zheng, Ran Song, Yanmei Zhang, Pinliang Jiang, Erwin A Vogler, Changjian Lin
: Blood compatibility of TiO2 nanotubes (TNTs) has been assessed in rabbit platelet-rich plasma (PRP), which combines activation of both blood plasma coagulation and platelets. We find that (i) amorphous TiO2 nanotubes (TNTs) with relatively larger outer diameters led to reduced platelet adhesion/activation, (ii) TNTs with relatively smaller outer diameters in a predominately rutile phase also inhibited platelet adhesion and activation, and (iii) a pervasive fibrin network formed on larger outer diameter TNTs in a predominately anatase phase...
January 13, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28092766/effect-of-multi-walled-carbon-nanotubes-on-phytotoxicity-of-sediments-contaminated-by-phenanthrene-and-cadmium
#16
Biao Song, Guangming Zeng, Jilai Gong, Peng Zhang, Jiaqin Deng, Canhui Deng, Jin Yan, Piao Xu, Cui Lai, Chen Zhang, Min Cheng
To implement effective control and abatement programs for contaminants accumulating in sediments, strategies are needed for evaluating the quality of amended sediments. In this study, phytotoxicity of the sediments contaminated by cadmium and phenanthrene was evaluated after in situ remediation with multi-walled carbon nanotubes (MWCNTs) as adsorbents. Adsorption experiments and measurement of aqueous concentrations of the contaminants in overlying water were used to investigate the remediation effectiveness from physical and chemical aspects...
January 9, 2017: Chemosphere
https://www.readbyqxmd.com/read/28092651/long-lasting-antinociceptive-effects-of-green-light-in-acute-and-chronic-pain-in-rats
#17
Mohab M Ibrahim, Amol Patwardhan, Kerry B Gilbraith, Aubin Moutal, Xiaofang Yang, Lindsey A Chew, Tally Largent-Milnes, T Philip Malan, Todd W Vanderah, Frank Porreca, Rajesh Khanna
Treatments for chronic pain are inadequate, and new options are needed. Nonpharmaceutical approaches are especially attractive with many potential advantages including safety. Light therapy has been suggested to be beneficial in certain medical conditions such as depression, but this approach remains to be explored for modulation of pain. We investigated the effects of light-emitting diodes (LEDs), in the visible spectrum, on acute sensory thresholds in naive rats as well as in experimental neuropathic pain...
February 2017: Pain
https://www.readbyqxmd.com/read/28092413/disruption-of-right-posterior-parietal-cortex-by-ctbs-alters-the-control-of-body-balance-in-quiet-stance
#18
David Kaulmann, Joachim Hermsdörfer, Leif Johannsen
Control of body balance relies on the integration of multiple sensory modalities. Lightly touching an earth-fixed reference augments the control of body sway. We aimed to advance the understanding of cortical integration of an afferent signal from light fingertip contact (LT) for the stabilisation of standing body balance. Assuming that right-hemisphere Posterior Parietal Cortex (rPPC) is involved in the integration and processing of touch for postural control, we expected that disrupting rPPC would attenuate any effects of light touch...
January 16, 2017: European Journal of Neuroscience
https://www.readbyqxmd.com/read/28091653/trivalent-f-elements-in-human-saliva-a-comprehensive-speciation-study-by-time-resolved-laser-induced-fluorescence-spectroscopy-and-thermodynamic-calculations
#19
Astrid Barkleit, Claudia Wilke, Anne Heller, Thorsten Stumpf, Atsushi Ikeda-Ohno
In the case of oral ingestion of radioactive contaminants, the first contact medium is saliva in the mouth. To gain a first insight into the interaction of radioactive contaminants in human saliva, the speciation of curium (Cm(iii)) and europium (Eu(iii)), i.e., trivalent f-elements, was investigated in different salivary media with time-resolved laser-induced fluorescence spectroscopy (TRLFS). The results indicate that these metal cations are primarily complexed with carbonates and phosphates, forming ternary complexes with a possible stoichiometry of 1 : 1 : 2 (M(iii) : carbonate : phosphate)...
January 16, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28088972/-critical-roles-of-matrix-metalloproteinases-secreted-by-leukemic-cells-in-the-pathogenesis-of-central-nervous-system-leukemia
#20
S R Feng, Z X Chen, J N Cen, H J Shen, Y Y Wang, L Yao
Objective: To observe the effects of matrix metalloproteinases (MMP)-2 and MMP-9 secreted by leukemic cells on tight junction proteins ZO-1, claudin-5 and occluding and the permeability of the blood-brain barrier (BBB) and explore the mechanisms of MMP-2 and MMP-9 in leukemic cell infiltration of the central nervous system (CNS). Methods: The mRNA expressions of MMP-2 and MMP-9 in leukemic cell lines SHI-1, HL-60 and U937 were detected by quantitative RT-PCR. The MMP inhibitor GM6001 was used to inhibit the secretion of MMP-2 and MMP-9...
December 14, 2016: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
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