keyword
MENU ▼
Read by QxMD icon Read
search

contact inhibition

keyword
https://www.readbyqxmd.com/read/28922632/antibacterial-and-anti-biofouling-coating-on-hydroxyapatite-surface-based-on-peptide-modified-tannic-acid
#1
Xiao Yang, Pei Huang, Haihuan Wang, Shuang Cai, Yixue Liao, Ziqin Mo, Xinyuan Xu, Chunmei Ding, Changsheng Zhao, Jianshu Li
Hydroxyapatite-based implant is widely used in the fields of artificial teeth and bones. However, the problem of microbial communities on hydroxyapatite surfaces remain a great challenge. In order to inhibit bacterial adhesion and activity in a long term, a salivary acquired pellicle (SAP) bioinspired tannic acid (SAP3-TA) is synthesized and characterized. The SAP inspired peptide sequence DDDEEK has a good adsorption performance on the surface of hydroxyapatite (HA). Tannic acid (TA) is a natural polyphenolic compound that can be extracted from green tea, and it has been proved to have an effective antibacterial property...
September 6, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28920003/cd226-natural-killer-cells-fail-to-establish-stable-contacts-with-cancer-cells-and-show-impaired-control-of-tumor-metastasis-in-vivo
#2
Ji Sung Kim, Bo Ram Shin, Hong Kyung Lee, Jae Hee Lee, Ki Hun Kim, Jeong Eun Choi, A Young Ji, Jin Tae Hong, Youngsoo Kim, Sang-Bae Han
CD226 is an activating receptor expressed on natural killer (NK) cells, CD8(+) T cells, and other immune cells. Upon binding to its ligands expressed on target cells, CD226 activates intracellular signaling that triggers cytokine production and degranulation in NK cells. However, the role of CD226 in contact dynamics between NK and cancer cells has remained unclear. Our time-lapse images showed that individual wild-type CD226(+) NK cells contacted B16F10 melanoma cells for 23.7 min, but Cd226(-/-) NK cells only for 12...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28919738/surface-modification-of-endovascular-stents-with-rosuvastatin-and-heparin-loaded-biodegradable-nanofibers-by-electrospinning
#3
Milka Janjic, Foteini Pappa, Varvara Karagkiozaki, Christakis Gitas, Kiriakos Ktenidis, Stergios Logothetidis
This study describes the development of drug-loaded nanofibrous scaffolds as a nanocoating for endovascular stents for the local and sustained delivery of rosuvastatin (Ros) and heparin (Hep) to injured artery walls after endovascular procedures via the electrospinning process. PURPOSE: The proposed hybrid covered stents can promote re-endothelialization; improve endothelial function; reduce inflammatory reaction; inhibit neointimal hyperplasia of the injured artery wall, due to well-known pleiotropic actions of Ros; and prevent adverse events such as in-stent restenosis (ISR) and stent thrombosis (ST), through the antithrombotic action of Hep...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28919501/structural-and-kinetics-characterization-of-the-f1f0-atp-synthase-dimer-new-repercussion-of-monomer-monomer-contact
#4
Mercedes Esparza-Perusquía, Sofía Olvera-Sánchez, Juan Pablo Pardo, Guillermo Mendoza-Hernández, Federico Martínez, Oscar Flores-Herrera
Ustilago maydis is an aerobic basidiomycete that fully depends on oxidative phosphorylation for its supply of ATP, pointing to mitochondria as a key player in the energy metabolism of this organism. Mitochondrial F1F0-ATP synthase occurs in supramolecular structures. In this work, we isolated the monomer (640kDa) and the dimer (1280kDa) and characterized their subunit composition and kinetics of ATP hydrolysis. Mass spectrometry revealed that dimerizing subunits e and g were present in the dimer but not in the monomer...
September 14, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28919310/cadherin-11-promotes-neural-crest-cell-spreading-by-reducing-intracellular-tension-mapping-adhesion-and-mechanics-in-neural-crest-explants-by-atomic-force-microscopy
#5
REVIEW
Carina Blaue, Jubin Kashef, Clemens M Franz
During development cranial neural crest cells (NCCs) display a striking transition from collective to single-cell migration, but the mechanisms enabling individual NCCs to separate from the neural crest tissue are still incompletely understood. In this study we have employed atomic force microscopy (AFM) to investigate potential adhesive and mechanical changes associated with the dissociation of individual cells from cohesive Xenopus NCC explants at early stages of migration. AFM-based single-cell force spectroscopy (SCFS) revealed a uniform distribution of cell-cell adhesion forces within NCC explants, including semi-detached leader cells in the process of delaminating from the explant edge...
September 14, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28918189/effects-of-temperature-and-peg-grafting-density-on-the-translocation-of-pegylated-nanoparticles-across-asymmetric-lipid-membrane
#6
Zuoheng Zhang, Xubo Lin, Ning Gu
Plasma membrane internalization of nanoparticles (NPs) is important for their biomedical applications such as drug-delivery carriers. On one hand, in order to improve their half-life in circulation, PEGylation has been widely used. However, it may hinder the NPs' membrane internalization ability. On the other hand, higher temperature could enhance the membrane permeability and may affect the NPs' ability to enter into or exit from cells. To make full use of their advantages, we systematically investigated the effects of temperature and PEG density on the translocation of PEGylated nanoparticles across the plasma asymmetric membrane of eukaryotic cells, using near-atom level coarse-grained molecular dynamics simulations...
September 6, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28916655/tethering-il2-to-its-receptor-il2r%C3%AE-enhances-anti-tumor-activity-and-expansion-of-natural-killer-nk92-cells
#7
Youssef Jounaidi, Joseph F Cotten, Keith W Miller, Stuart A Forman
Interleukin-2 (IL2) is an immunostimulatory cytokine for key immune cells including T cells and natural killer (NK) cells. Systemic IL2 supplementation could enhance NK-mediated immunity in a variety of diseases ranging from neoplasms to viral infection. However, its systemic use is restricted by its serious side effects and limited efficacy due to activation of T regulatory cells (Tregs). IL2 signaling is mediated through interactions with a multi-subunit receptor complex containing IL2Rα, IL2Rβ and IL2Rγ...
September 15, 2017: Cancer Research
https://www.readbyqxmd.com/read/28916520/rubrocerebellar-feedback-loop-isolates-the-interposed-nucleus-as-an-independent-processor-of-corollary-discharge-information-in-mice
#8
Christy S Beitzel, Brenda D Houck, Samantha M Lewis, Abigail L Person
Understanding cerebellar contributions to motor coordination requires deeper insight into how the output structures of the cerebellum, the cerebellar nuclei, integrate their inputs and influence downstream motor pathways. The magnocellular red nucleus (RNm), a brainstem premotor structure, is a major target of the interposed nucleus (IN), and has also been described in previous studies to send feedback collaterals to the cerebellum. Because such a pathway is in a key position to provide motor efferent information to the cerebellum, satisfying predictions about the use of corollary discharge in cerebellar computations, we studied it in mice of both sexes...
September 15, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28916328/primary-hippocampal-neuronal-cell-death-induction-after-acute-and-repeated-paraquat-exposures-mediated-by-ache-variants-alteration-and-cholinergic-and-glutamatergic-transmission-disruption
#9
Javier Del Pino, Paula Moyano, Gloria Gómez Díaz, María José Anadon, Maria Jesus Diaz, José Manuel García, Margarita Lobo, Adela Pelayo, Emma Sola, María Teresa Frejo
Paraquat (PQ) is a widely used non-selective contact herbicide shown to produce memory and learning deficits after acute and repeated exposure similar to those induced in Alzheimer's disease (AD). However, the complete mechanisms through which it induces these effects are unknown. On the other hand, cholinergic and glutamatergic systems, mainly in the hippocampus, are involved on learning, memory and cell viability regulation. An alteration of hippocampal cholinergic or glutamatergic transmissions or neuronal cell loss may induce these effects...
September 12, 2017: Toxicology
https://www.readbyqxmd.com/read/28914615/apoptosis-generates-mechanical-forces-that-close-the-lens-vesicle-in-the-chick-embryo
#10
Alina Oltean, Larry A Taber
During the initial stages of eye development, optic vesicles grow laterally outward from both sides of the forebrain and come into contact with the surrounding surface ectoderm (SE). Within the region of contact, these layers then thicken locally to create placodes and invaginate to form the optic cup (primitive retina) and lens vesicle (LV), respectively. This paper examines the biophysical mechanisms involved in LV formation, which consists of three phases: (1) lens placode formation; (2) invagination to create the lens pit (LP); and (3) closure to form a complete ellipsoidally shaped LV...
September 15, 2017: Physical Biology
https://www.readbyqxmd.com/read/28914053/construction-of-functional-coating-with-durable-and-broad-spectrum-antibacterial-potential-based-on-mussel-inspired-dendritic-polyglycerol-and-in-situ-formed-copper-nanoparticles
#11
Mingjun Li, Lingyan Gao, Christoph Schlaich, Jianguang Zhang, Ievgen S Donskyi, Guozhi Yu, Wenzhong Li, Zhaoxu Tu, Jens Rolff, Tanja Schwerdtle, Rainer Haag, Nan Ma
A novel surface coating with durable broad-spectrum antibacterial ability was prepared based on mussel-inspired dendritic polyglycerol (MI-dPG) embedded with copper nanoparticles (Cu NPs). The functional surface coating is fabricated via a facile dip-coating process followed by in-situ reduction of copper ions with a MI-dPG coating to introduce Cu NPs into the coating matrix. This coating has been demonstrated efficient long term antibacterial properties against Escherichia coli (E. coli), Staphylococcus aureus (S...
September 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28912796/a-low-cost-imaging-method-for-the-temporal-and-spatial-colorimetric-detection-of-free-amines-on-maize-root-surfaces
#12
Truc H Doan, Tu A Doan, Michael J Kangas, AdreAnna E Ernest, Danny Tran, Christina L Wilson, Andrea E Holmes, Erin L Doyle, Tessa L Durham Brooks
Plant root exudates are important mediators in the interactions that occur between plants and microorganisms in the soil, yet much remains to be learned about spatial and temporal variation in their production. This work outlines a method utilizing a novel colorimetric paper to detect spatial and temporal changes in the production of nitrogen-containing compounds on the root surface. While existing methods have made it possible to conduct detailed analysis of root exudate composition, relatively less is known about where in the root system exudates are produced and how this localization changes as the root grows...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28912528/extracellular-vesicles-derived-from-t-regulatory-cells-suppress-t-cell-proliferation-and-prolong-allograft-survival
#13
Sistiana Aiello, Federica Rocchetta, Lorena Longaretti, Silvia Faravelli, Marta Todeschini, Linda Cassis, Francesca Pezzuto, Susanna Tomasoni, Nadia Azzollini, Marilena Mister, Caterina Mele, Sara Conti, Matteo Breno, Giuseppe Remuzzi, Marina Noris, Ariela Benigni
We have previously shown that rat allogeneic DC, made immature by adenoviral gene transfer of the dominant negative form of IKK2, gave rise in-vitro to a unique population of CD4(+)CD25(-) regulatory T cells (dnIKK2-Treg). These cells inhibited Tcell response in-vitro, without needing cell-to-cell contact, and induced kidney allograft survival prolongation in-vivo. Deep insight into the mechanisms behind dnIKK2-Treg-induced suppression of Tcell proliferation remained elusive. Here we document that dnIKK2-Treg release extracellular vesicles (EV) riched in exosomes, fully accounting for the cell-contact independent immunosuppressive activity of parent cells...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28912435/turning-behaviors-of-t-cells-climbing-up-ramp-like-structures-are-regulated-by-myosin-light-chain-kinase-activity-and-lamellipodia-formation
#14
Kwang Hoon Song, Jaehyun Lee, Hong-Ryul Jung, HyoungJun Park, Junsang Doh
T cells navigate diverse microenvironments to perform immune responses. Micro-scale topographical structures within the tissues, which may inherently exist in normal tissues or may be formed by inflammation or injury, can influence T cell migration, but how T cell migration is affected by such topographical structures have not been investigated. In this study, we fabricated ramp-like structures with a 5 μm height and various slopes, and observed T cells climbing up the ramp-like structures. T cells encountering the ramp-like structures exhibited MLC accumulation near head-tail junctions contacting the ramp-like structures, and made turns to the direction perpendicular to the ramp-like structures...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28911019/early-arabidopsis-root-hair-growth-stimulation-by-pathogenic-strains-of-pseudomonas-syringae
#15
Tamara Pecenková, Martin Janda, Jitka Ortmannová, Vladimíra Hajná, Zuzana Stehlíková, Viktor Žárský
Background and Aims: Selected beneficial Pseudomonas spp. strains have the ability to influence root architecture in Arabidopsis thaliana by inhibiting primary root elongation and promoting lateral root and root hair formation. A crucial role for auxin in this long-term (1week), long-distance plant-microbe interaction has been demonstrated. Methods: Arabidopsis seedlings were cultivated in vitro on vertical plates and inoculated with pathogenic strains Pseudomonas syringae pv...
September 1, 2017: Annals of Botany
https://www.readbyqxmd.com/read/28904872/treatment-of-renal-anemia-erythropoiesis-stimulating-agents-and-beyond
#16
REVIEW
Patrick Biggar, Gheun-Ho Kim
Anemia, complicating the course of chronic kidney disease, is a significant parameter, whether interpreted as subjective impairment or an objective prognostic marker. Renal anemia is predominantly due to relative erythropoietin (EPO) deficiency. EPO inhibits apoptosis of erythrocyte precursors. Studies using EPO substitution have shown that increasing hemoglobin (Hb) levels up to 10-11 g/dL is associated with clinical improvement. However, it has not been unequivocally proven that further intensification of erythropoiesis stimulating agent (ESA) therapy actually leads to a comprehensive benefit for the patient, especially as ESAs are potentially associated with increased cerebro-cardiovascular events...
September 2017: Kidney Research and Clinical Practice
https://www.readbyqxmd.com/read/28903323/the-pancreatic-tumor-microenvironment-drives-changes-in-mirna-expression-that-promote-cytokine-production-and-inhibit-migration-by-the-tumor-associated-stroma
#17
Song Han, David H Gonzalo, Michael Feely, Daniel Delitto, Kevin E Behrns, Mark Beveridge, DongYu Zhang, Ryan Thomas, Jose G Trevino, Thomas D Schmittgen, Steven J Hughes
The pancreatic adenocarcinoma (PDAC) microenvironment is largely comprised of fibrotic tumor associated stroma (TAS) that contributes to the lethal biology of PDAC. microRNA (miRNA) are small non-coding RNAs that regulate gene expression. We hypothesized that interactions between PDAC cells and TAS cells within the microenvironment modulate miRNA expression and thus, tumor biology. We observed that miR-205 and members of the miR-200 family (miR-200a, -200b, -200c, -141 and miR-429) were exclusively expressed in PDAC cells, consistent with an epithelial miRNA signature, while miR-145 and miR-199 family members (miR-199a and -199b) were solely expressed in TAS cells, consistent with a stromal miRNA signature...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28902018/mechanism-of-proliferation-of-cultured-human-corneal-endothelial-cells
#18
Takeshi Joko, Atsushi Shiraishi, Takeshi Kobayashi, Yuichi Ohashi, Shigeki Higashiyama
Because human corneal endothelial cells (HCECs) do not proliferate once the endothelial monolayer has formed, corneal wound healing is believed to be mediated by cell enlargement or migration, rather than by proliferation. However, the cellular mechanisms involved in wound healing by HCECs have not been fully determined. In this review, we focus on the effects of promyelocytic leukemia zinc finger (PLZF), a DNA-binding transcription factor, and transforming growth factor (TGF)-β2 on the proliferation and migration of cultured HCECs...
September 7, 2017: Cornea
https://www.readbyqxmd.com/read/28901501/mtor-inhibition-reduces-growth-and-adhesion-of-hepatocellular-carcinoma-cells-in%C3%A2-vitro
#19
Tobias Engl, Jochen Rutz, Sebastian Maxeiner, Eva Juengel, Frederik Roos, Wael Khoder, Wolf O Bechstein, Karen Nelson, Igor Tsaur, Axel Haferkamp, Roman A Blaheta
Mechanistic target of rapamycin (mTOR) signaling is typically increased in hepatocellular carcinoma (HCC). A panel of HCC cell lines (HepG2, Hep3B and HuH6) was exposed to various concentrations of the mTOR inhibitors, everolimus and temsirolimus, in order to investigate their effects on cell growth, clonal formation, cell cycle progression, and adhesion and chemotactic migration using MTT and clonal cell growth assays, fluorometric detection of cell cycle phases and a Boyden chamber assay. In addition, integrin α and β adhesion receptors were analyzed by flow cytometry and blocking studies using function blocking monoclonal antibodies were conducted to explore functional relevance...
August 31, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28893939/human-t-cell-leukaemia-virus-type-1-parasitism-and-pathogenesis
#20
REVIEW
Charles R M Bangham, Masao Matsuoka
Human T-cell leukaemia virus type 1 (HTLV-1) causes not only adult T-cell leukaemia-lymphoma (ATL), but also inflammatory diseases including HTLV-1-associated myelopathy/tropical spastic paraparesis. HTLV-1 transmits primarily through cell-to-cell contact, and generates abundant infected cells in the host in order to survive and transmit to a new host. The resulting high proviral load is closely associated with the development of ATL and inflammatory diseases. To increase the number of infected cells, HTLV-1 changes the immunophenotype of infected cells, induces proliferation and inhibits apoptosis through the cooperative actions of two viral genes, tax and HTLV-1 bZIP factor (HBZ)...
October 19, 2017: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
keyword
keyword
90164
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"