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https://www.readbyqxmd.com/read/28728008/pen-on-paper-strategy-for-point-of-care-testing-rapid-prototyping-of-fully-written-microfluidic-biosensor
#1
Zedong Li, Fei Li, Yue Xing, Zhi Liu, Minli You, Yingchun Li, Ting Wen, Zhiguo Qu, Xiao Ling Li, Feng Xu
Paper-based microfluidic biosensors have recently attracted increasing attentions in point-of-care testing (POCT) territories benefiting from their affordable, accessible and eco-friendly features, where technologies for fabricating such biosensors are preferred to be equipment free, easy-to-operate and capable of rapid prototyping. In this work, we developed a pen-on-paper (PoP) strategy based on two custom-made pens, i.e., a wax pen and a conductive-ink pen, to fully write paper-based microfluidic biosensors through directly writing both microfluidic channels and electrodes...
July 1, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28728007/direct-immune-detection-of-cortisol-by-chemiresistor-graphene-oxide-sensor
#2
Yo-Han Kim, Kyungmin Lee, Hunsang Jung, Hee Kyung Kang, Jihoon Jo, In-Kyu Park, Hyun Ho Lee
In this study, a biosensor to detect a stress biomarker of cortisol using cortisol monoclonal antibody (c-Mab) covalently immobilized on reduced graphene oxide (rGO) channel as electrical sensing element was demonstrated. Highly specific immune-recognition between the c-Mab and the cortisol was identified and characterized on a basis of resistance change at the rGO channel based chemiresistor sensor achieving the limit of detection of 10pg/mL (27.6 pM). In addition, cortisol concentrations of real human salivary sample and buffer solution of rat adrenal gland acute slices, which could secret the cortisol induced by adrenocorticotropic hormone (ACTH), were directly measured by the chemiresistor corresponding to the specific sensing of the cortisol...
July 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28724363/comparing-patient-and-healthcare-worker-experiences-during-a-dengue-outbreak-in-singapore-understanding-the-patient-journey-and-the-introduction-of-a-point-of-care-test-poct-toward-better-care-delivery
#3
Qinghui Tan, Zoe J-L Hildon, Shweta Singh, Jin Jing, Tun Linn Thein, Richard Coker, Hubertus J M Vrijhoef, Yee Sin Leo
BACKGROUND: In the aftermath of an upsurge in the number of dengue cases in 2013 and 2014, the SD BIOLINE Dengue Duo rapid diagnostic Point-of-Care Test (POCT) kit was introduced in Tan Tock Seng Hospital, Singapore in June 2013. It is known that the success of POCT usage is contingent on its implementation within the health system. We evaluated health services delivery and the Dengue Duo rapid diagnostic test kit application in Singapore from healthcare workers' perspectives and patient experiences of dengue at surge times...
July 19, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28721805/automated-one-pot-radiosynthesis-of-11c-s-adenosyl-methionine
#4
Florencia Zoppolo, Williams Porcal, Patricia Oliver, Eduardo Osvaldo Savio, Henry Engler
BACKGROUND: Glycine-N-methyl transferase is an enzyme overexpressed in some neoplastic tissues. It catalyses the methylation of glycine using S-adenosyl methionine (SAM) as co-enzyme. SAM is involved in a great variety of biochemical processes, including transmethylation reactions. Thus, [11C]SAM could be used to evaluate transmethylation activity in tumours. The only method reported for [11C]SAM synthesis is an enzymatic process with several limitations. We propose a new chemical method to obtain [11C]SAM, through a one-pot synthesis...
July 18, 2017: Current Radiopharmaceuticals
https://www.readbyqxmd.com/read/28711526/xpert-hiv-1-point-of-care-test-for-neonatal-diagnosis-of-hiv-in-the-birth-testing-programme-of-a-maternity-hospital-a-field-evaluation-study
#5
Karl-Günter Technau, Louise Kuhn, Ashraf Coovadia, Pamela M Murnane, Gayle Sherman
BACKGROUND: Point-of-care testing (POCT) among HIV-exposed infants might improve linkage to care relative to laboratory-based testing (LABT). We evaluated HIV-1 POCT at birth in the context of universal LABT in a maternity hospital and describe our implementation experience. METHODS: We did a field evaluation study between Oct 1, 2014, and April 30, 2016, at the urban Rahima Moosa Mother and Child Hospital (RMMCH), Johannesburg, South Africa. We aimed to sample consecutive neonates at birth with POCT (Cepheid Xpert HIV-1 Qualitative test) and compared results with those of LABT (Roche COBAS TaqMan HIV-1 Qualitative test) with respect to performance in terms of sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and Cohen's κ coefficient, result return, antiretroviral treatment (ART) initiation, and coverage...
July 12, 2017: Lancet HIV
https://www.readbyqxmd.com/read/28708567/%C3%AE-defensin-point-of-care-test-for-diagnosis-of-prosthetic-joint-infections-neglected-role-of-laboratory-and-clinical-pathologists
#6
REVIEW
Lorenzo Drago, Marco Toscano, Lorenza Tacchini, Giuseppe Banfi
Periprosthetic joint infection (PJI) is a serious complication that may occur after native joint replacement leading to a severe health and economic burden. Currently, due to several confounding factors, PJI is difficult to diagnose. Today, a multidisciplinary approach is indispensable to correctly define a periprosthetic joint infection; indeed, tissue histology, microbiology cultures and clinical findings are used together to achieve this goal. Analysis of α-defensin is commonly used for PJI diagnosis, as it allows the rapid detection of α-defensin present in the synovial fluid following a microbial infection...
July 14, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28705266/a-new-method-for-blood-nt-probnp-determination-based-on-a-near-infrared-point-of-care-testing-device-with-high-sensitivity-and-wide-scope
#7
Xiao Guang Zhang, Yao Gen Shu, Ju Gao, Xuan Wang, Li Peng Liu, Meng Wang, Yu Xi Cao, Yi Zeng
OBJECTIVE: To develop a rapid, highly sensitive, and quantitative method for the detection of NT-proBNP levels based on a near-infrared point-of-care diagnostic (POCT) device with wide scope. METHODS: The lateral flow assay (LFA) strip of NT-proBNP was first prepared to achieve rapid detection. Then, the antibody pairs for NT-proBNP were screened and labeled with the near-infrared fluorescent dye Dylight-800. The capture antibody was fixed on a nitrocellulose membrane by a scribing device...
June 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28702608/point-of-care-testing-applications-of-3d-printing
#8
REVIEW
Ho Nam Chan, Ming Jun Andrew Tan, Hongkai Wu
Point-of-care testing (POCT) devices fulfil a critical need in the modern healthcare ecosystem, enabling the decentralized delivery of imperative clinical strategies in both developed and developing worlds. To achieve diagnostic utility and clinical impact, POCT technologies are immensely dependent on effective translation from academic laboratories out to real-world deployment. However, the current research and development pipeline is highly bottlenecked owing to multiple restraints in material, cost, and complexity of conventionally available fabrication techniques...
July 12, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28685569/point-of-care-assay-of-telomerase-activity-at-single-cell-level-via-gas-pressure-readout
#9
Yanjun Wang, Luzhu Yang, Baoxin Li, Chaoyong James Yang, Yan Jin
Detection of telomerase activity at the single-cell level is one of the central challenges in cancer diagnostics and therapy. Herein, we describe a facile and reliable point-of-care testing (POCT) strategy for detection of telomerase activity via a portable pressure meter. Telomerase primer (TS) was immobilized onto the surface of magnetic beads (MBs), and then was elongated to a long single-stranded DNA by telomerase. The elongated (TTAGGG)n repeat unit hybridized with several short PtNP-functionalized complementary DNA (PtNPs-cDNA), which specifically enriched PtNPs onto the surfaces of magnetic beads (MBs), which were separated using a magnet...
July 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28677648/advancing-point-of-care-poc-testing-using-human-saliva-as-liquid-biopsy
#10
REVIEW
Rabia Sannam Khan, Zohaib Khurshid, Faris Yahya Ibrahim Asiri
Salivary diagnostics is an emerging field for the encroachment of point of care technology (PoCT). The necessity of the development of point-of-care (PoC) technology, the potential of saliva, identification and validation of biomarkers through salivary diagnostic toolboxes, and a broad overview of emerging technologies is discussed in this review. Furthermore, novel advanced techniques incorporated in devices for the early detection and diagnosis of several oral and systemic diseases in a non-invasive, easily-monitored, less time consuming, and in a personalised way is explicated...
July 4, 2017: Diagnostics
https://www.readbyqxmd.com/read/28662147/development-and-evaluation-of-an-up-converting-phosphor-technology-based-lateral-flow-assay-for-the-rapid-simultaneous-detection-of-vibrio-cholerae-serogroups-o1-and-o139
#11
Min Hao, Pingping Zhang, Baisheng Li, Xiao Liu, Yong Zhao, Hailing Tan, Chongyun Sun, Xiaochen Wang, Xinrui Wang, Haiyan Qiu, Duochun Wang, Baowei Diao, Huaiqi Jing, Ruifu Yang, Biao Kan, Lei Zhou
Vibrio cholerae serogroups O1 and O139 are etiological agents of cholera, a serious and acute diarrheal disease, and rapid detection of V. cholerae is a key method for preventing and controlling cholera epidemics. Here, a point of care testing (POCT) method called Vch-UPT-LF, which is an up-converting phosphor technology-based lateral flow (UPT-LF) assay with a dual-target detection mode, was developed to detect V. cholerae O1 and O139 simultaneously from one sample loading. Although applying an independent reaction pair made both detection results for the two Vch-UPT-LF detection channels more stable, the sensitivity slightly declined from 104 to 105 colony-forming units (CFU) mL-1 compared with that of the single-target assay, while the quantification ranges covering four orders of magnitude were maintained...
2017: PloS One
https://www.readbyqxmd.com/read/28660121/point-of-care-testing-to-promote-cardiovascular-disease-risk-assessment-a-proof-of-concept-study
#12
Kunal N Karmali, Tiffany Brown, Thomas Sanchez, Timothy Long, Stephen D Persell
Updated cholesterol guidelines emphasize multivariable cardiovascular disease (CVD) risk estimation to guide treatment decision-making in primary prevention. This study tested the preliminary feasibility, acceptability and efficacy of point-of-care testing (POCT) and quantitative CVD risk assessment in high-risk adults to increase guideline-recommended statin use in primary prevention. Participants were aged 40-75 years, without CVD or diabetes mellitus, and potentially-eligible for consideration of statins based on estimated 10-year CVD risk from last-measured risk factor levels in the electronic health record...
September 2017: Preventive Medicine Reports
https://www.readbyqxmd.com/read/28651513/the-performance-of-flash-glucose-monitoring-in-critically-ill-patients-with-diabetes
#13
Paolo Ancona, Glenn M Eastwood, Luca Lucchetta, Elif I Ekinci, Rinaldo Bellomo, Johan Mårtensson
OBJECTIVE: Frequent glucose monitoring may improve glycaemic control in critically ill patients with diabetes. We aimed to assess the accuracy of a novel subcutaneous flash glucose monitor (FreeStyle Libre [Abbott Diabetes Care]) in these patients. METHODS: We applied the FreeStyle Libre sensor to the upper arm of eight patients with diabetes in the intensive care unit and obtained hourly flash glucose measurements. Duplicate recordings were obtained to assess test-retest reliability...
June 2017: Critical Care and Resuscitation: Journal of the Australasian Academy of Critical Care Medicine
https://www.readbyqxmd.com/read/28650611/integrating-target-responsive-hydrogel-with-pressuremeter-readout-enables-simple-sensitive-user-friendly-quantitative-point-of-care-testing
#14
Dan Liu, Shasha Jia, Huimin Zhang, Yanli Ma, Zhichao Guan, Jiuxing Li, Zhi Zhu, Tianhai Ji, Chaoyong James Yang
Point-of-care testing (POCT) with the advantages of speed, simplicity, and low cost, as well as no need for instrumentation, is critical for the measurement of analytes in a variety of environments lacking access to laboratory infrastructure. In the present study, a hydrogel pressure-based assay for quantitative POCT was developed by integrating a target-responsive hydrogel with pressuremeter readout. The target-responsive hydrogels were constructed with DNA grafted linear polyacrylamide and the cross-linking DNA for selective target recognition...
July 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28649724/evaluation-of-the-uriscan-2-acr-strip-to-estimate-the-urine-albumin-creatinine-ratios
#15
Sangeun Lim, Hui-Jin Yu, Seungjun Lee, Hyosoon Park, Min-Jung Kwon, Hee-Yeon Woo
BACKGROUND: The urine albumin/creatinine ratio (ACR) test is used to screen patients with chronic diseases, such as diabetes, hypertension and cardiovascular diseases that put them at an increased risk of developing kidney disease. Here, we evaluated the performance of the URiSCAN 2 ACR Strip (URiSCAN; YD diagnostics, Yongin, Korea), a semiquantitative point-of-care testing (POCT) assay, and we compared to an existing POCT assay and a quantitative assay. MATERIALS AND METHODS: A total of 1,020 random urine specimens were analyzed using the semiquantitative URiSCAN 2 ACR Strip and semiquantitative CLINITEK Microalbumin 2 Strip (CLINITEK; Siemens, New York, USA)...
June 26, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28649023/stability-enhanced-repeatability-improved-parylene-c-passivated-on-qcm-sensor-for-aptt-measurement
#16
Yuchen Yang, Wei Zhang, Zhen Guo, Zhiqi Zhang, Hongnan Zhu, Ruhong Yan, Lianqun Zhou
Determination of blood clotting time is essential in monitoring therapeutic anticoagulants. In this work, Parylene-C passivated on quartz crystal microbalance (P-QCM) was developed for the activated partial thromboplastin time (aPTT) measurement. Compared with typical QCM, P-QCM possessed a hydrophobic surface and sensitive frequency response to viscoelastic variations on electrode surface. Fibrin could be adsorbed effectively, due to the hydrophobicity of the P-QCM surface. Comparing with typical QCM, the peak-to-peak value (PPV) of P-QCM was increased by 1...
June 16, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28638471/rapid-detection-of-avian-influenza-virus-by-fluorescent-diagnostic-assay-using-an-epitope-derived-peptide
#17
Duong Tuan Bao, Do Thi Hoang Kim, Hyun Park, Bui Thi Cuc, Nguyen Minh Ngoc, Nguyen Thi Phuong Linh, Nguyen Chien Huu, Trinh Thi Thuy Tien, Nguyen Thi Viet Anh, Tung Dao Duy, Chom-Kyu Chong, Seung-Taek Yu, Do-Young Choi, Seon-Ju Yeo
Currently, the point of care testing (POCT) is not fully developed for subtype-specific avian influenza virus detection. In this study, an H5N1 hemaglutinin 1 (HA1) epitope (P0: KPNDAINF) and three modified peptides (P1: KPNTAINF, P2: KPNGAINF, P3: KPNDAINDAINF) were evaluated as POCT elements for rapid detection of avian influenza virus. Based on modeling predictions by Autodock Vina, binding affinity varied depending on alteration of one amino acid in these peptides. The binding energy of P2 indicated its potential for a strong interaction with HA...
2017: Theranostics
https://www.readbyqxmd.com/read/28632479/use-and-acceptability-of-salivary-hepatitis-c-virus-testing-in-an-english-young-offender-institution
#18
Sophie Candfield, Mannampallil I Samuel, David Ritchie, Candice McDonald, Michael Brady, Chris Taylor
People held in prison are at a high risk of having hepatitis C virus (HCV) and there is a public health drive in the UK to increase HCV testing in prisons and Young Offender Institutions (YOIs), with opt-out testing. There is an oral antibody test for HCV; this project aims to determine its acceptability in an English YOI setting. This project offered HCV oral point-of-care testing (POCT) using the OraQuick® test to 107 male young offenders attending a sexual health service at an English YOI, monitoring HCV positivity and evaluating acceptability...
January 1, 2017: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/28619082/assessing-changes-in-knowledge-attitude-and-practices-on-dengue-diagnosis-and-management-among-primary-care-physicians-after-the-largest-dengue-epidemic-in-singapore
#19
Junxiong Pang, Zoe Jane-Lara Hildon, Tun Linn Thein, Jing Jin, Yee Sin Leo
BACKGROUND: Dengue results in high morbidity and mortality globally. The knowledge, attitude and practices (KAP) of dengue management, including diagnosis, among primary care physicians (PCPs) are important to reduce dengue transmission and burden. However, there is a lack of understanding on the impact of dengue epidemic on dengue management. Hence, the aim of this study is to examine the changes in KAP on dengue management among PCPs before and after the largest dengue epidemic in 2013 in Singapore...
June 15, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28615273/analysis-of-the-potential-for-point-of-care-test-to-enable-individualised-treatment-of-infections-caused-by-antimicrobial-resistant-and-susceptible-strains-of-neisseria-gonorrhoeae-a-modelling-study
#20
Katy Me Turner, Hannah Christensen, Elisabeth J Adams, David McAdams, Helen Fifer, Anthony McDonnell, Neil Woodford
OBJECTIVE: To create a mathematical model to investigate the treatment impact and economic implications of introducing an antimicrobial resistance point-of-care test (AMR POCT) for gonorrhoea as a way of extending the life of current last-line treatments. DESIGN: Modelling study. SETTING: England. POPULATION: Patients accessing sexual health services. INTERVENTIONS: Incremental impact of introducing a hypothetical AMR POCT that could detect susceptibility to previous first-line antibiotics, for example, ciprofloxacin or penicillin, so that patients are given more tailored treatment, compared with the current situation where all patients are given therapy with ceftriaxone and azithromycin...
June 14, 2017: BMJ Open
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