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Yingjun Ma, Jun Chen, Danping Chen, Yuzhi Xu, Li Zhang, Zong Dai, Xiaoyong Zou
Specific nucleic acids amplification at a constant and mild temperature is important for imaging assay of endogenous microRNAs (miRNAs) in living cells. Duplex-specific nuclease (DSN) is attractive in one-step isothermal assay of miRNA; however, its intrinsic limitations of low amplification specificity and high reaction temperature greatly restrict the application scope. Herein, we present a short-probe-based DSN signal amplification (spDSNSA) strategy enabling analysis of miRNAs at body temperature with significantly high specificity...
August 15, 2018: Talanta
Alessio Mancini, Giampaolo Esposto, Silvana Manfrini, Silvia Rilli, Gessica Tinti, Giuseppe Carta, Laura Petrolati, Matteo Vidali, Simone Barocci
The aim of this retrospective study is to evaluate the reliability and robustness of six glucose meters for point-of-care testing in our wards using a brand-new protocol. During a 30-days study period a total of 50 diabetes patients were subjected to venous blood sampling and glucose meter blood analysis. The results of six glucose meters were compared with our laboratory reference assay. GlucoMen Plus (Menarini) with the 82% of acceptable results was the most robust glucose meter. Even if the Passing-Bablok analysis demonstrates the presence of constant systematic errors and the Bland-Altman test highlighted a possible overestimation, the surveillance error grid analysis showed that this glucose meter can be used safely...
May 1, 2018: Journal of Diabetes Science and Technology
Xin-Yu Cao, Fan-Zhi Kong, Qiang Zhang, Wei-Wen Liu, Xiao-Ping Liu, Guo-Qing Li, Ran Zhong, Liu-Yin Fan, Hua Xiao, Cheng-Xi Cao
As a vital enzyme, alkaline phosphatase (ALP) has great clinical significance in diagnoses of bone or liver cancer, bone metastases, rickets, and extrahepatic biliary obstruction. However, there is still no really portable chip for the ALP assay in blood. Herein, a simple electrophoresis titration (ET) model was developed for ALP detection via a moving reaction boundary (MRB). In the model, ALP catalyzed the dephosphorylation of a 4-methylumbelliferyl phosphate disodium salt (4-MUP) substrate in the cathode well to 4-methylumbelliferone ([4-MU]-) with a negative charge and blue fluorescence under UV excitation...
May 21, 2018: Lab on a Chip
Nan-Fu Chiu, Chi-Chu Chen, Cheng-Du Yang, Yu-Sheng Kao, Wei-Ren Wu
In this study, we propose a modified gold nanoparticle-graphene oxide sheet (AuNP-GO) nanocomposite to detect two different interactions between proteins and hybrid nanocomposites for use in biomedical applications. GO sheets have high bioaffinity, which facilitates the attachment of biomolecules to carboxyl groups and has led to its use in the development of sensing mechanisms. When GO sheets are decorated with AuNPs, they introduce localized surface plasmon resonance (LSPR) in the resonance energy transfer of spectral changes...
May 16, 2018: Nanoscale Research Letters
Jesse M Pines, Mark S Zocchi, Caitlin Carter, Charles Z Marriott, Matthew Bernard, Leah H Warner
STUDY OBJECTIVE: Point-of-care testing (POCT) is a commonly used technology that hastens the time to laboratory results in emergency departments (ED). We evaluated an ED-based POCT program on ED length of stay and time to care, coupled with qualitative interviews of local ED stakeholders. METHODS: We conducted a mixed-methods study (2012-16) to examine the impact of point-of-care testing in a single, community ED. The quantiative analysis involved an observational before-after study comparing time to laboratory test result (POC troponin or POC chemistry) and ED length of stay after implementation of POCT, using a propensity-weighted interrupted time series analysis (ITSA)...
May 13, 2018: Academic Emergency Medicine: Official Journal of the Society for Academic Emergency Medicine
S Bhaduri, H Curtis, H McClean, A K Sullivan
This national audit of 142 clinics demonstrated that the majority of clinics surveyed had policies and agreed clinical practice for alcohol and recreational drug enquiry, as well as documentation of HIV test refusal, although this was not the case in 24% of clinics as regards alcohol usage, 21% of clinics as regards recreational drugs use and 43% of clinics as regards chemsex usage. Regarding management of HIV test refusal, there was no policy or agreed practice in 13% of clinics with respect to men having sex with men (MSM) attenders, and in 18% of clinics for heterosexual attenders...
January 1, 2018: International Journal of STD & AIDS
Veerle Matheeussen, Viviane Van Hoof, Katherine Loens, Christine Lammens, Anouk Vanderstraeten, Samuel Coenen, Chris C Butler, Paul Little, Theo J M Verheij, Herman Goossens, Margareta Ieven
C-reactive protein (CRP) is a biomarker widely used for disease severity assessment and treatment of inflammatory conditions. Point-of-care testing (POCT) devices should ideally be rapid and provide similar results to standard tests done in laboratories. Two thousand nine hundred twenty-two serum samples were obtained from adult patients presenting to primary care with symptoms of lower respiratory infection in a European diagnostic study. The analytic performance of the CRP QuikRead POCT device (Orion Diagnostica) was evaluated by comparing results with a central laboratory method (Dimension Vista, Siemens), with both tests performed in a laboratory setting...
May 10, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Feng-Fei Li, Yun Xie, Bing-Yin Shi, Min Niu, Hui Guo, Yan Cao, Bing-Li Liu, Reng-Na Yan, Xiao-Fei Su, Jin-Dan Wu, Dan-Feng Zhang, Li-Ming Chen, Jian-Hua Ma
BACKGROUND:  The blood glucose point-of-care testing (POCT) system is important in the decision-making process involving patients suspected of having hypoglycemia. To investigate the real world of the POCT system being used in teaching hospitals in China. METHODS: The survey was conducted by Hisend Research Group from May 2015 to July 2015 in four teaching hospitals in China. The survey questions were referred to the ISO 15197:2013 standard requirements for the use of the POCT system in a hospital setting...
May 9, 2018: Expert Review of Medical Devices
Yanxue Cai, Keren Kang, Qianru Li, Yu Wang, Xiaowei He
A reliable lateral flow immunoassay (LFIA) based on a facile one-step synthesis of single microspheres in combining with immunochromatography technique was developed to establish a new point-of-care test (POCT) for the rapid and early detection of cardiac troponin I (cTnI), a kind of cardiac specific biomarker for acute myocardial infarction (AMI). The double layered microspheres with clear core-shell structures were produced using soap-free emulsion polymerization method with inexpensive compounds (styrene and acrylic acid)...
May 7, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Jakeline Ribeiro Barbosa, Vanessa Faria Cortes, Moyra Machado Portilho, Juliana Custódio Miguel, Vanessa Alves Marques, Cristianne Sousa Bezerra, Natalia Vasconcelos de Souza, Danielle Malta Lima, Jeová Keny Baima Colares, Lia Laura Lewis-Ximenez, Elisabeth Lampe, Livia Melo Villar
AIMS: Point of care testing (POCT) has been used for hepatitis B and C diagnosis in general population, but little is known about the influence of clinical conditions in the accuracy of these assays. This study aims to evaluate the performance of POCTs for detection of hepatitis B virus surface antigen (HBsAg) and antibodies to Hepatitis C Virus (anti-HCV) in Chronic Kidney Disease (CKD) patients. METHODS: A total of 286 subjects were included in this study. HBsAg and anti-HCV were detected using commercial EIAs and four POCTs: HBsAg (WAMA Imuno-Rápido HBsAg and VIKIA HBsAg) and anti-HCV (DOLES HCV teste rápido and WAMA Imuno-Rápido anti-HCV) in serum and whole blood...
May 5, 2018: Journal of Clinical Pathology
Andreas Bietenbeck, Wolf J Geilenkeuser, Frank Klawonn, Michael Spannagl, Matthias Nauck, Astrid Petersmann, Markus A Thaler, Christof Winter, Peter B Luppa
BACKGROUND: Determination of blood glucose concentration is one of the most important measurements in clinical chemistry worldwide. Analyzers in central laboratories (CL) and point-of-care tests (POCT) are both frequently used. In Germany, regular participation in external quality assessment (EQA) schemes is mandatory for laboratories performing glucose testing. METHODS: Glucose testing data from the two German EQAs "Reference Institute for Bioanalytics" (RfB) and "INSTAND - Gesellschaft zur Förderung der Qualitätssicherung in medizinischen Laboratorien" (Instand) were analyzed from 2012 to 2016...
May 1, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
Andres I Vecino-Ortiz, Simon D Goldenberg, Sam T Douthwaite, Chih-Yuan Cheng, Rebecca E Glover, Catherine Mak, Elisabeth J Adams
Patients with respiratory infections are often managed presumptively until confirmation of infection status. We assessed the impact of introducing the Enigma® MiniLab™ FluAB-RSV point-of-care test (POCT) on patients admitted with a suspected respiratory virus driven illness in an acute pediatric ward. This utilized a before and after design (respiratory viral seasons 2013/14 versus 2014/15). Following POCT implementation, oseltamivir prescribing increased in patients with influenza (OR = 12.7, P = 0...
April 4, 2018: Diagnostic Microbiology and Infectious Disease
Marie Brionne-François, Anne Bauters, Christine Mouton, Sophie Voisin, Claire Flaujac, Agnès Le Querrec, Dominique Lasne
Point of care testing (POCT) must comply with regulatory requirements according to standard EN ISO 22870, which identify biologists as responsible for POCT. INR for vitamin K antagonists (VKAs) monitoring is a test frequently performed in haemostasis laboratories. Bedside INR is useful in emergency room, in particular in case of VKAs overdosage but also for specific populations of patients like paediatrics or geriatrics. INR POCT devices are widely used at home by the patients for self-testing, but their use in the hospital by the clinical staff for bedside measurement is growing, with devices which now comply with standard for POCT accreditation for hospital use...
April 27, 2018: Annales de Biologie Clinique
Kelli Dulaney, Kenneth Hohmeier, Cindy Fisher, Lindsey Cardosi, Mike Wasson
OBJECTIVES: To assess pharmacists' perceptions of point-of-care testing (POCT) and treatment for influenza and streptococcus pharyngitis in a community pharmacy setting. A secondary objective was to explore the correlation between demographic data and survey responses. METHODS: An anonymous electronic Likert-type-scale questionnaire was sent to pharmacists in a division of a large national supermarket chain pharmacy in western Tennessee, Mississippi, and Arkansas...
April 21, 2018: Journal of the American Pharmacists Association: JAPhA
Mitsuko Seki, Chang-Ki Kim, Satoshi Hayakawa, Satoshi Mitarai
Smear-negative and drug-resistant cases of tuberculosis (TB) disease necessitate the development of new diagnostic methods, especially in resource-limited settings. To improve the current TB situations, sensitive and specific TB point-of-care tests (POCTs) should be developed. This review addresses the current status of TB, novel diagnostic methodologies for TB, and the impact of those new diagnostics on TB control in such situations. Moreover, the perspective of TB management based on laboratory examinations is described...
April 19, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Michael Marks, Tommy Esau, Rowena Asugeni, Relmah Harrington, Jason Diau, Hilary Toloka, James Asugeni, Eimhin Ansbro, Anthony W Solomon, David Maclaren, Michelle Redman-Maclaren, David C W Mabey
INTRODUCTION: The human treponematoses comprise venereal syphilis and the three non-venereal or endemic treponematoses yaws, bejel, and pinta. Serological assays remain the most common diagnostic method for all treponemal infections. Point-of-care tests (POCTs) for syphilis and yaws allow testing without further development of infrastructure in populations where routine laboratory facilities are not available. Alongside the test's performance characteristics assessed through diagnostic evaluation, it is important to consider broader issues when rolling out a POCT...
April 2018: PLoS Neglected Tropical Diseases
Lu Shi, Jing Lei, Bei Zhang, Baoxin Li, Chaoyong James Yang, Yan Jin
The upregulation of microRNA (miRNA) is highly related with some kinds of tumor, such as breast, prostate, lung, and pancreatic cancers. Therefore, for an important tumor biomarker, the point-of-care testing (POCT) of miRNA is of significant importance and is in great demand for disease diagnosis and clinical prognoses. Herein, a POCT assay for miRNA detection was developed via a portable pressure meter. Two hairpin DNA probes, H1 and H2, were ingeniously designed and functionalized with magnetic beads (MBs) and platinum nanoparticles (PtNPs), respectively, to form MBs-H1 and PtNPs-H2 complexes...
April 6, 2018: ACS Applied Materials & Interfaces
Simone M Gonçalves, Karina B Gomes, Maria das G Carvalho, Henrique P B Magalhães, Edna A Reis, Iêda de Fátima O Silva
BACKGROUND: Among cardiovascular diseases (CVD), acute coronary syndrome (ACS) is the main manifestation, corresponding to signs and symptoms that occur with ischemia and outcome of angina or acute myocardial infarction (AMI). The aim of this study was to investigate the performance of biochemical markers eligible in a chest pain protocol, using Point of care Test (POCT), in patients in a reference emergency room. METHODS: In this study, 1380 medical records of patients of both genders were evaluated, ranked by applying chest pain protocol using the Manchester Triage System (MTS)...
April 2, 2018: Journal of Clinical Laboratory Analysis
Marianne Abele-Horn, Ellen Pantke, Tim Eckmanns
Germany's position on prescribing antibiotics is among the lower third in Europe. There are some countries that prescribe remarkably fewer antibiotics. In Germany, the number of out-patient antibiotic prescriptions is too high for patients with low-grade infections and non-bacterial infections. Often, broad-spectrum antibiotics are prescribed instead of narrow-spectrum antibiotics. For in-patients, perioperative prophylaxis is given for too long.In this article, different ways to reduce antibiotic prescriptions are introduced...
March 26, 2018: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz
Kartika Saraswati, Nicholas P J Day, Mavuto Mukaka, Stuart D Blacksell
BACKGROUND: Diagnosing scrub typhus clinically is difficult, hence laboratory tests play a very important role in diagnosis. As performing sophisticated laboratory tests in resource-limited settings is not feasible, accurate point-of-care testing (POCT) for scrub typhus diagnosis would be invaluable for patient diagnosis and management. Here we summarise the existing evidence on the accuracy of scrub typhus POCTs to inform clinical practitioners in resource-limited settings of their diagnostic value...
March 26, 2018: PLoS Neglected Tropical Diseases
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