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https://www.readbyqxmd.com/read/27910892/dynamic-microenvironment-induces-phenotypic-plasticity-of-esophageal-cancer-cells-under-flow
#1
Gizem Calibasi Kocal, Sinan Güven, Kira Foygel, Aaron Goldman, Pu Chen, Shiladitya Sengupta, Ramasamy Paulmurugan, Yasemin Baskin, Utkan Demirci
Cancer microenvironment is a remarkably heterogeneous composition of cellular and non-cellular components, regulated by both external and intrinsic physical and chemical stimuli. Physical alterations driven by increased proliferation of neoplastic cells and angiogenesis in the cancer microenvironment result in the exposure of the cancer cells to elevated levels of flow-based shear stress. We developed a dynamic microfluidic cell culture platform utilizing eshopagael cancer cells as model cells to investigate the phenotypic changes of cancer cells upon exposure to fluid shear stress...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27904893/assay-for-listeria-monocytogenes-cells-in-whole-blood-using-isotachophoresis-and-recombinase-polymerase-amplification
#2
Charbel Eid, Juan G Santiago
We present a new approach which enables lysis, extraction, and detection of inactivated Listeria monocytogenes cells from blood using isotachophoresis (ITP) and recombinase polymerase amplification (RPA). We use an ITP-compatible alkaline and proteinase K approach for rapid and effective lysis. We then perform ITP purification to separate bacterial DNA from whole blood contaminants using a microfluidic device that processes 25 μL sample volume. Lysis, mixing, dispensing, and on-chip ITP purification are completed in a total of less than 50 min...
December 1, 2016: Analyst
https://www.readbyqxmd.com/read/27902940/a-novel-lab-on-chip-platform-with-integrated-solid-phase-pcr-and-supercritical-angle-fluorescence-saf-microlens-array-for-highly-sensitive-and-multiplexed-pathogen-detection
#3
Tran Quang Hung, Wai Hoe Chin, Yi Sun, Anders Wolff, Dang Duong Bang
Solid-phase PCR (SP-PCR) has become increasingly popular for molecular diagnosis and there have been a few attempts to incorporate SP-PCR into lab-on-a-chip (LOC) devices. However, their applicability for on-line diagnosis is hindered by the lack of sensitive and portable on-chip optical detection technology. In this paper, we addressed this challenge by combining the SP-PCR with super critical angle fluorescence (SAF) microlens array embedded in a microchip. We fabricated miniaturized SAF microlens array as part of a microfluidic chamber in thermoplastic material and performed multiplexed SP-PCR directly on top of the SAF microlens array...
November 12, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27900430/visible-and-red-emissive-molecular-beacons-for-optical-temperature-measurements-and-quality-control-in-diagnostic-assays-utilizing-temperature-dependent-amplification-reactions
#4
Zerrin Fidan, Andy Wende, Ute Resch-Genger
Quality control requirements imposed on assays used in clinical diagnostics and point-of-care-diagnostic testing (POCT), utilizing amplification reactions performed at elevated temperatures of 35 to 95 °C are very stringent. As the temperature of a reaction vessel has a large impact on the specificity and sensitivity of the amplification reaction, simple tools for local in situ temperature sensing and monitoring are required for reaction and assay control. We describe here a platform of stem-and-loop structured DNA hairpins (molecular beacons, MBs), absorbing and emitting in the visible and red spectral region, rationally designed for precise temperature measurements in microfluidic assays for POCT, and their application for temperature measurements in a common DNA-based molecular biological assay utilizing thermophilic helicase-dependent amplification (tHDA)...
November 29, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27900374/spatially-resolved-microfluidic-stimulation-of-lymphoid-tissue-ex-vivo
#5
Ashley E Ross, Maura C Belanger, Jacob F Woodroof, Rebecca R Pompano
The lymph node is a structurally complex organ of the immune system, whose dynamic cellular arrangements are thought to control much of human health. Currently, no methods exist to precisely stimulate substructures within the lymph node or analyze local stimulus-response behaviors, making it difficult to rationally design therapies for inflammatory disease. Here we describe a novel integration of live lymph node slices with a microfluidic system for local stimulation. Slices maintained the cellular organization of the lymph node while making its core experimentally accessible...
November 30, 2016: Analyst
https://www.readbyqxmd.com/read/27900024/acute-effect-of-lactic-acid-on-tumor-endothelial-cell-metabolic-coupling-in-the-tumor-microenvironment
#6
Guanqun Zhu, Degui Wang, Shenqian Li, Xuecheng Yang, Yanwei Cao, Yonghua Wang, Haitao Niu
The present study aimed to systematically analyze alterations in the expression of mitochondrial-associated proteins in human bladder cancer T24 cells co-cultured with tumor-associated human umbilical vein endothelial cells (HUVECs), and to investigate the characteristics of bladder cancer cell energy metabolism. The present study used the following techniques: A co-culture system of T24 cells and HUVECs was constructed using a microfluidic chip as a 3D co-culture system; the concentration of lactic acid in the medium of the cells was determined using an automatic microplate reader; a qualitative analysis of mitochondria-associated protein expression was performed by immunofluorescent staining; and a quantitative analysis of mitochondrial-associated protein expression was conducted using western blotting...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27899572/c3-symmetric-opioid-scaffolds-are-ph-responsive-dna-condensation-agents
#7
Natasha McStay, Zara Molphy, Alan Coughlan, Attilio Cafolla, Vickie McKee, Nicholas Gathergood, Andrew Kellett
Herein we report the synthesis of tripodal C3-symmetric opioid scaffolds as high-affinity condensation agents of duplex DNA. Condensation was achieved on both supercoiled and canonical B-DNA structures and identified by agarose electrophoresis, viscosity, turbidity and atomic force microscopy (AFM) measurements. Structurally, the requirement of a tris-opioid scaffold for condensation is demonstrated as both di- (C2-symmetric) and mono-substituted (C1-symmetric) mesitylene-linked opioid derivatives poorly coordinate dsDNA...
November 28, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27896351/on-chip-integration-of-organic-synthesis-and-hplc-ms-analysis-for-monitoring-stereoselective-transformations-at-the-micro-scale
#8
Josef J Heiland, Rico Warias, Carsten Lotter, Laura Mauritz, Patrick J W Fuchs, Stefan Ohla, Kirsten Zeitler, Detlev Belder
We present a microfluidic system, seamlessly integrating microflow and microbatch synthesis with a HPLC/nano-ESI-MS functionality on a single glass chip. The microfluidic approach allows to efficiently steer and dispense sample streams down to the nanoliter-range for studying reactions in quasi real-time. In a proof-of-concept study, the system was applied to explore amino-catalyzed reactions, including asymmetric iminium-catalyzed Friedel-Crafts alkylations in microflow and micro confined reaction vessels.
November 29, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27892475/droplet-merging-on-a-lab-on-a-chip-platform-by-uniform-magnetic-fields
#9
V B Varma, A Ray, Z M Wang, Z P Wang, R V Ramanujan
Droplet microfluidics offers a range of Lab-on-a-chip (LoC) applications. However, wireless and programmable manipulation of such droplets is a challenge. We address this challenge by experimental and modelling studies of uniform magnetic field induced merging of ferrofluid based droplets. Control of droplet velocity and merging was achieved through uniform magnetic field and flow rate ratio. Conditions for droplet merging with respect to droplet velocity were studied. Merging and mixing of colour dye + magnetite composite droplets was demonstrated...
November 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27886051/a-laminar-flow-based-microfluidic-tesla-pump-via-lithography-enabled-3d-printing
#10
Mohammed-Baker Habhab, Tania Ismail, Joe Fujiou Lo
Tesla turbine and its applications in power generation and fluid flow were demonstrated by Nicholas Tesla in 1913. However, its real-world implementations were limited by the difficulty to maintain laminar flow between rotor disks, transient efficiencies during rotor acceleration, and the lack of other applications that fully utilize the continuous flow outputs. All of the aforementioned limits of Tesla turbines can be addressed by scaling to the microfluidic flow regime. Demonstrated here is a microscale Tesla pump designed and fabricated using a Digital Light Processing (DLP) based 3D printer with 43 µm lateral and 30 µm thickness resolutions...
November 23, 2016: Sensors
https://www.readbyqxmd.com/read/27883138/characterization-of-size-dependent-mechanical-properties-of-tip-growing-cells-using-a-lab-on-chip-device
#11
Chengzhi Hu, Gautam Munglani, Hannes Vogler, Tohnyui Ndinyanka Fabrice, Naveen Shamsudhin, Falk K Wittel, Christoph Ringli, Ueli Grossniklaus, Hans J Herrmann, Bradley J Nelson
Quantification of mechanical properties of tissues, living cells, and cellular components is crucial for the modeling of plant developmental processes such as mechanotransduction. Pollen tubes are tip-growing cells that provide an ideal system to study the mechanical properties at the single cell level. In this article, a lab-on-a-chip (LOC) device is developed to quantitatively measure the biomechanical properties of lily (Lilium longiflorum) pollen tubes. A single pollen tube is fixed inside the microfluidic chip at a specific orientation and subjected to compression by a soft membrane...
November 24, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27883132/microfluidic-pdms-on-paper-pop-devices
#12
Jin-Wen Shangguan, Yu Liu, Jian-Bin Pan, Bi-Yi Xu, Jing-Juan Xu, Hong-Yuan Chen
In this paper, we propose a generalized concept of microfluidic polydimethylsiloxane (PDMS) on paper (POP) devices, which combines well the merits of paper chips and PDMS chips. First, we optimized the conditions for accurate PDMS spatial patterning on paper, based on screen printing and a high temperature enabled superfast curing technique, which enables PDMS patterning to an accuracy of tens of microns in less than ten seconds. This, in turn, makes it available for seamless, reversible and reliable integration of the resulting paper layer with other PDMS channel structures...
November 24, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27882460/development-of-a-specific-substrate-inhibitor-panel-liver-on-a-chip-for-evaluation-of-cytochrome-p450-activity
#13
A A Zakhariants, O A Burmistrova, M Y Shkurnikov, A A Poloznikov, D A Sakharov
We developed a cytochrome P450 substrate-inhibitor panel for preclinical in vitro evaluation of drugs in a 3D histotypical microfluidic cell model of human liver (liver-on-a-chip technology). The concentrations of substrates and inhibitors were optimized to ensure reliable detection of the principal metabolites by HPLC-mass-spectroscopy. The selected specific substrate-inhibitor pairs, namely bupropion/2-phenyl-2-(1-piperidinyl)propane) for evaluation of CYP2B6B activity, tolbutamide/sulfaphenazole for CYP2C9, omeprazole/(+)-N-benzylnirvanol for CYP2C19, and testosterone/ketoconazole for CYP3A4, enable reliable evaluation of the drug metabolism pathway...
November 23, 2016: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27875995/high-throughput-gene-expression-quantification-of-grapevine-defense-responses-in-the-field-using-microfluidic-dynamic-arrays
#14
Marie-Cécile Dufour, Noël Magnin, Bernard Dumas, Sophie Vergnes, Marie-France Corio-Costet
BACKGROUND: The fight against grapevine diseases due to biotrophic pathogens usually requires the massive use of chemical fungicides with harmful environmental effects. An alternative strategy could be the use of compounds able to stimulate plant immune responses which significantly limit the development of pathogens in laboratory conditions. However, the efficiency of this strategy in natura is still insufficient to be included in pest management programs. To understand and to improve the mode of action of plant defense stimulators in the field, it is essential to develop reliable tools that describe the resistance status of the plant upon treatment...
November 22, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27875619/automated-leukocyte-processing-by-microfluidic-deterministic-lateral-displacement
#15
Curt I Civin, Tony Ward, Alison M Skelley, Khushroo Gandhi, Zendra Peilun Lee, Christopher R Dosier, Joseph L D'Silva, Yu Chen, MinJung Kim, James Moynihan, Xiaochun Chen, Lee Aurich, Sergei Gulnik, George C Brittain, Diether J Recktenwald, Robert H Austin, James C Sturm
We previously developed a Deterministic Lateral Displacement (DLD) microfluidic method in silicon to separate cells of various sizes from blood (Davis et al., Proc Natl Acad Sci 2006;103:14779-14784; Huang et al., Science 2004;304:987-990). Here, we present the reduction-to-practice of this technology with a commercially produced, high precision plastic microfluidic chip-based device designed for automated preparation of human leukocytes (white blood cells; WBCs) for flow cytometry, without centrifugation or manual handling of samples...
November 22, 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27871055/microfluidic-device-for-label-free-quantitation-and-distinction-of-bladder-cancer-cells-from-the-blood-cells-using-micro-machined-silicon-based-electrical-approach-suitable-in-urinalysis-assays
#16
Seied Ali Hosseini, Somayeh Zanganeh, Elaheh Akbarnejad, Fatemeh Salehi, Mohammad Abdolahad
This paper introduces an integrated microfluidic chip as a promising tool to measure the concentration of bladder cancer cells (BCC) in urine samples. Silicon microchannels were used as trapping gates for both floated BCC and leukocytes which are found in the urine of patients. By the assistance of the gold electrodes patterned at the bottom of the micro gates, the capacitance of captured cancerous and blood cells were measured. Different membrane capacitance between BCC and leukocyte was the indicative signal for diagnosing the nature of captured cells in a urine like solution...
November 13, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27870841/tracking-the-dynamics-of-circulating-tumour-cell-phenotypes-using-nanoparticle-mediated-magnetic-ranking
#17
Mahla Poudineh, Peter M Aldridge, Sharif Ahmed, Brenda J Green, Leyla Kermanshah, Vivian Nguyen, Carmen Tu, Reza M Mohamadi, Robert K Nam, Aaron Hansen, Srikala S Sridhar, Antonio Finelli, Neil E Fleshner, Anthony M Joshua, Edward H Sargent, Shana O Kelley
Profiling the heterogeneous phenotypes of rare circulating tumour cells (CTCs) in whole blood is critical to unravelling the complex and dynamic properties of these potential clinical markers. This task is challenging because these cells are present at parts per billion levels among normal blood cells. Here we report a new nanoparticle-enabled method for CTC characterization, called magnetic ranking cytometry, which profiles CTCs on the basis of their surface expression phenotype. We achieve this using a microfluidic chip that successfully processes whole blood samples...
November 21, 2016: Nature Nanotechnology
https://www.readbyqxmd.com/read/27869150/skin-on-a-chip-model-simulating-inflammation-edema-and-drug-based-treatment
#18
Maierdanjiang Wufuer, GeonHui Lee, Woojune Hur, Byoungjun Jeon, Byung Jun Kim, Tae Hyun Choi, SangHoon Lee
Recent advances in microfluidic cell cultures enable the construction of in vitro human skin models that can be used for drug toxicity testing, disease study. However, current in vitro skin model have limitations to emulate real human skin due to the simplicity of model. In this paper, we describe the development of 'skin-on-a-chip' to mimic the structures and functional responses of the human skin. The proposed model consists of 3 layers, on which epidermal, dermal and endothelial components originated from human, were cultured...
November 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27868126/new-insights-into-mammalian-signaling-pathways-using-microfluidic-pulsatile-inputs-and-mathematical-modeling
#19
REVIEW
M Sumit, S Takayama, J J Linderman
Temporally modulated input mimics physiology. This chemical communication strategy filters the biochemical noise through entrainment and phase-locking. Under laboratory conditions, it also expands the observability space for downstream responses. A combined approach involving microfluidic pulsatile stimulation and mathematical modeling has led to deciphering of hidden/unknown temporal motifs in several mammalian signaling pathways and has provided mechanistic insights, including how these motifs combine to form distinct band-pass filters and govern fate regulation under dynamic microenvironment...
November 21, 2016: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/27866008/organs-on-chips-research-and-commercial-perspectives
#20
REVIEW
Aarathi Balijepalli, Vaibhav Sivaramakrishan
Traditional preclinical drug testing methods utilize animal models to predict pharmacology and toxicology profiles. However, the data obtained from such methods cannot be directly extrapolated to humans and often do not provide a safe starting dose for first-in-human studies. To overcome these limitations, researchers have developed organs-on-chips - microfluidic devices that can mimic the cellular architecture and physiology more accurately than conventional methods. Because accurate organ-level interactions can be achieved with these devices, they have the potential to provide a realistic determination of a drug's pharmacokinetics, pharmacodynamics and toxicity profile...
November 16, 2016: Drug Discovery Today
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