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https://www.readbyqxmd.com/read/29144515/detection-of-sepsis-in-patient-blood-samples-using-cd64-expression-in-a-microfluidic-cell-separation-device
#1
Ye Zhang, Wenjie Li, Yun Zhou, Amanda Johnson, Amanda Venable, Ahmed Hassan, John Griswold, Dimitri Pappas
A microfluidic affinity separation device was developed for the detection of sepsis in critical care patients. An affinity capture method was developed to capture cells based on changes in CD64 expression in a single, simple microfluidic chip for sepsis detection. Both sepsis patient samples and a laboratory CD64+ expression model were used to validate the microfluidic assay. Flow cytometry analysis showed that the chip cell capture had a linear relationship with CD64 expression in laboratory models. The Sepsis Chip detected an increase in upregulated neutrophil-like cells when the upregulated cell population is as low as 10% of total cells spiked into commercially available aseptic blood samples...
November 16, 2017: Analyst
https://www.readbyqxmd.com/read/29144379/a-digital-microfluidics-platform-for-loop-mediated-isothermal-amplification-detection
#2
Beatriz Jorge Coelho, Bruno Veigas, Hugo Águas, Elvira Fortunato, Rodrigo Martins, Pedro Viana Baptista, Rui Igreja
Digital microfluidics (DMF) arises as the next step in the fast-evolving field of operation platforms for molecular diagnostics. Moreover, isothermal schemes, such as loop-mediated isothermal amplification (LAMP), allow for further simplification of amplification protocols. Integrating DMF with LAMP will be at the core of a new generation of detection devices for effective molecular diagnostics at point-of-care (POC), providing simple, fast, and automated nucleic acid amplification with exceptional integration capabilities...
November 16, 2017: Sensors
https://www.readbyqxmd.com/read/29143053/recent-advances-in-microfluidic-technologies-for-cell-to-cell-interaction-studies
#3
REVIEW
Mario Rothbauer, Helene Zirath, Peter Ertl
Microfluidic cell cultures are ideally positioned to become the next generation of in vitro diagnostic tools for biomedical research, where key biological processes such as cell signalling and dynamic cell-to-cell interactions can be reliably analysed under reproducible physiological cell culture conditions. In the last decade, a large number of microfluidic cell analysis systems have been developed for a variety of applications including drug target optimization, drug screening and toxicological testing. More recently, advanced in vitro microfluidic cell culture systems have emerged that are capable of replicating the complex three-dimensional architectures of tissues and organs and thus represent valid biological models for investigating the mechanism and function of human tissue structures, as well as studying the onset and progression of diseases such as cancer...
November 16, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/29138494/drying-in-a-microfluidic-chip-experiments-and-simulations
#4
Paolo Fantinel, Oshri Borgman, Ran Holtzman, Lucas Goehring
We present an experimental micro-model of drying porous media, based on microfluidic cells made of arrays of pillars on a regular grid, and complement these experiments with a matching two-dimensional pore-network model of drying. Disorder, or small-scale heterogeneity, was introduced into the cells by randomly varying the radii of the pillars. The microfluidic chips were filled with a volatile oil and then dried horizontally, such that gravitational effects were excluded. The experimental and simulated drying rates and patterns were then compared in detail, for various levels of disorder...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29136313/in-situ-scatheless-cell-detachment-reveals-connections-between-adhesion-strength-and-viability-at-single-cell-resolution
#5
Sifeng Mao, Wanling Zhang, Qiushi Huang, Masqooh Khan, Haifang Li, Katsumi Uchiyama, Jin-Ming Lin
Single-cell biology provides insights to some of the most fundamental processes in biology and promotes the understanding of life's mysteries. And as the technologies to study single-cells expand, they will require sophisticated analytical tools to make sense of various behaviors and components of single-cells as well as their relations in adherent tissue culture. In this paper, we revealed cell heterogeneity and uncovered the connections between cell adhesion strength and cell viability at single-cell resolution by extracting single adherent cells of interest from standard tissue culture via a microfluidic chip based Live Single-Cell Extractor (LSCE)...
November 14, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29134742/innovative-technologies-for-point-of-care-testing-of-viral-hepatitis%C3%A2-in-low-resource-and-decentralized-settings
#6
Léa Duchesne, Karine Lacombe
According to the Global Burden of Diseases, chronic viral hepatitis B and C are one of the most challenging global health conditions that rank among the first causes of morbidity and mortality worldwide. Low and middle income countries are particularly affected by the health burden associated with HBV or HCV infection. One major gap in efficiently addressing the issue of viral hepatitis is universal screening. However, the costs and chronic lack of human resources for using traditional screening strategies based on serology and molecular biology preclude any scaling-up...
November 13, 2017: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/29132051/high-purity-capture-of-ctcs-based-on-micro-beads-enhanced-isolation-by-size-of-epithelial-tumor-cells-iset-method
#7
Na Sun, Xinpan Li, Zhili Wang, Yuzhi Li, Renjun Pei
In this paper, we develop a low-cost size-based microfluidic chip using conventional polycarbonate membrane to isolate CTCs from blood, and propose a strategy to increase the capture efficiency before cell filtration by a size enlargement method utilizing modified microbeads specifically binding to CTCs. Up to 91% of target cells were isolated from whole blood samples using our microfluidic capture system at a flow rate of 1mL/min. Moreover, a WBC depletion process is introduced which greatly decreases the WBC retaining on the filter membrane...
November 7, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29131592/a-paper-plastic-hybrid-microfluidic-device-for-smartphone-based-colorimetric-analysis-of-urine
#8
M Jalal Uddin, Gyeong Jun Jin, Joon Sub Shim
In this work, a disposable paper-plastic hybrid microfluidic lab-on-a-chip (LOC) has been developed and successfully applied for the colorimetric measurement of urine by the smartphone-based optical platform using an "UrineAnalysis" Android app. The developed device was cost-effectively implemented as a stand-alone hybrid LOC by incorporating the paper-based conventional reagent test strip inside the plastic-based LOC microchannel. The LOC device quantitatively investigated the small volume (40 µL) of urine analytes for the colorimetric reaction of glucose, protein, pH and red blood cell (RBC) in integration with the finger-actuating micro pump...
November 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29128921/a-microfluidic-in-line-elisa-for-measuring-secreted-protein-under-perfusion
#9
Qiyue Luan, Stacey Cahoon, Agnes Wu, Shyam Sundhar Bale, Martin Yarmush, Abhinav Bhushan
Recent progress in the development of microfluidic microphysiological systems such as 'organs-on-chips' and microfabricated cell culture is geared to simulate organ-level physiology. These tissue models leverage microengineering technologies that provide capabilities of presenting cultured cells with input signals in a more physiologically relevant context such as perfused flow. Proteins that are secreted from cells have important information about the health of the cells. Techniques to quantify cellular proteins include mass spectrometry to ELISA (enzyme-linked immunosorbent assay)...
November 11, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/29128714/multiplex-immunoassays-using-virus-tethered-gold-microspheres-by-dc-impedance-based-flow-cytometry
#10
Jihun Rho, Woohyuk Jang, Inseong Hwang, Dahye Lee, Chang Heon Lee, Taek Dong Chung
Bead-based multiplex immunoassays for common use require enhanced sensitivity and effective prevention of non-specific adsorption, as well as miniaturization of the detection device. In this work, we have implemented virus-tethered gold microspheres for multiplex immunoassay applications, employing a DC impedance-based flow cytometer as a detection element. The advantages of virus-tethered gold microspheres, including excellent prevention of non-specific adsorption, are extended to signal enhancement arising from the large quantity of antibody loading on each virion, and to flexible movement of filamentous virus...
November 7, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29127692/coagulation-measurement-from-whole-blood-using-vibrating-optical-fiber-in-a-disposable-cartridge
#11
Yusuf Samet Yaraş, Ali Bars Gündüz, Gökhan Sağlam, Selim Ölçer, Fehmi Civitçi, İbrahim Baris, Göksenin Yaralioğlu, Hakan Urey
In clinics, blood coagulation time measurements are performed using mechanical measurements with blood plasma. Such measurements are challenging to do in a lab-on-a-chip (LoC) system using a small volume of whole blood. Existing LoC systems use indirect measurement principles employing optical or electrochemical methods. We developed an LoC system using mechanical measurements with a small volume of whole blood without requiring sample preparation. The measurement is performed in a microfluidic channel where two fibers are placed inline with a small gap in between...
November 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29127057/quantitative-analysis-of-clonidine-and-ephedrine-by-a-microfluidic-system-on-chip-electromembrane-extraction-followed-by-high-performance-liquid-chromatography
#12
Mahroo Baharfar, Yadollah Yamini, Shahram Seidi, Monireh Karami
In this work, a microfluidic device was developed for on-chip electromembrane extraction of trace amounts of ephedrine (EPH) and clonidine (CLO) in human urine and plasma samples followed by HPLC-UV analysis. Two polymethylmethacrylate plates were used as substrates and a microchannel was carved in each plate. The microchannel channel on the underneath plate provided the flow pass of the sample solution and the one on the upper plate dedicated to a compartment for the stagnant acceptor phase. A piece of polypropylene sheet was impregnated by an organic solvent and mounted between the two parts of the chip device...
November 2, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29126108/laboratory-scale-photobiotechnology-current-trends-and-future-perspectives
#13
Holger Morschett, Varun Loomba, Gregor Huber, Wolfgang Wiechert, Eric von Lieres, Marco Oldiges
Phototrophic bioprocesses are a promising puzzle piece in future bio-economy concepts but mostly fail for economic reasons, yet. Besides other aspects, this is mainly attributed to the omnipresent issue of optimal light supply impeding scale-up and -down of phototrophic processes according to classic established concepts. This MiniReview examines two current trends in photobiotechnology, namely microscale cultivation and modeling and simulation. Micro photobioreactors are a valuable and promising trend with microfluidic chips and microtiter plates as predominant design concepts...
November 8, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29124848/quantitative-chemical-biosensing-by-bacterial-chemotaxis-in-microfluidic-chips
#14
Clémence Roggo, Cristian Picioreanu, Xavier Richard, Christian Mazza, Harald van Lintel, Jan Roelof van der Meer
Whole-cell bacterial bioreporters are proposed as alternatives to chemical analysis of, e.g., pollutants in environmental compartments. Commonly based on reporter gene induction, bioreporters produce a detectable signal within 30 min to a few hours after exposure to the chemical target, which is impractical for applications aiming at a fast response. In an attempt to attain faster readout but maintain flexibility of chemical targeting, we explored the concept for quantitative chemical sensing by bacterial chemotaxis...
November 10, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/29121462/design-of-lubricant-infused-surfaces
#15
Daniel John Preston, Youngsup Song, Zhengmao Lu, Dion S Antao, Evelyn N Wang
Lubricant infused surfaces (LIS) are a recently-developed and promising approach to fluid repellency for applications in biology, microfluidics, thermal management, lab-on-a-chip, and beyond. The design of LIS has been explored in past work in terms of surface energies which need to be determined empirically for each interface in a given system. Here, we developed an approach which predicts a priori whether an arbitrary combination of solid and lubricant will repel a given impinging fluid. This model was validated with experiments performed in our work as well as in literature and was subsequently used to develop a new framework for LIS with distinct design guidelines...
November 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29119176/a-novel-microfluidic-microelectrode-chip-for-a-significantly-enhanced-monitoring-of-npy-receptor-activation-in-live-mode
#16
Franziska D Zitzmann, Heinz-Georg Jahnke, Felix Nitschke, Annette G Beck-Sickinger, Bernd Abel, Detlev Belder, Andrea A Robitzki
Lab-on-a-chip devices that combine, e.g. chemical synthesis with integrated on-chip analytics and multi-compartment organ-on-a-chip approaches, are a fast and attractive evolving research area. While integration of appropriate cell models in microfluidic setups for monitoring the biological activity of synthesis products or test compounds is already in focus, the integration of label-free bioelectronic analysis techniques is still poorly realized. In this context, we investigated the capabilities of impedance spectroscopy as a non-destructive real-time monitoring technique for adherent cell models in a microfluidic setup...
November 9, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/29118739/resolving-species-level-changes-in-a-representative-soil-bacterial-community-using-microfluidic-quantitative-pcr
#17
Hannah Kleyer, Robin Tecon, Dani Or
Rapid advances in genome sequencing technologies enable determination of relative bacterial abundances and community composition, yet, changes at the species level remain difficult to detect despite importance for certain ecological inferences. We present a method for extraction and direct quantification of species composition of a predefined multispecies bacterial community using microfluidic-based quantitative real-time PCR (qPCR). We employ a nested PCR approach based on universal 16S rRNA gene pre-amplification followed by detection and quantification of absolute abundance of bacterial species using microfluidic array of parallel singleplex qPCR reactions...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29118385/enhanced-physicochemical-properties-of-polydimethylsiloxane-based-microfluidic-devices-and-thin-films-by-incorporating-synthetic-micro-diamond
#18
Sidra Waheed, Joan M Cabot, Niall P Macdonald, Umme Kalsoom, Syamak Farajikhah, Peter C Innis, Pavel N Nesterenko, Trevor W Lewis, Michael C Breadmore, Brett Paull
Synthetic micro-diamond-polydimethylsiloxane (PDMS) composite microfluidic chips and thin films were produced using indirect 3D printing and spin coating fabrication techniques. Microfluidic chips containing up to 60 wt% micro-diamond were successfully cast and bonded. Physicochemical properties, including the dispersion pattern, hydrophobicity, chemical structure, elasticity and thermal characteristics of both chip and films were investigated. Scanning electron microscopy indicated that the micro-diamond particles were embedded and interconnected within the bulk material of the cast microfluidic chip, whereas in the case of thin films their increased presence at the polymer surface resulted in a reduced hydrophobicity of the composite...
November 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29117571/single-cell-virology-on-chip-investigation-of-viral-infection-dynamics
#19
Feng Guo, Sixing Li, Mehmet Umut Caglar, Zhangming Mao, Wu Liu, Andrew Woodman, Jamie J Arnold, Claus O Wilke, Tony Jun Huang, Craig E Cameron
We have developed a high-throughput, microfluidics-based platform to perform kinetic analysis of viral infections in individual cells. We have analyzed thousands of individual poliovirus infections while varying experimental parameters, including multiplicity of infection, cell cycle, viral genotype, and presence of a drug. We make several unexpected observations masked by population-based experiments: (1) viral and cellular factors contribute uniquely and independently to viral infection kinetics; (2) cellular factors cause wide variation in replication start times; and (3) infections frequently begin later and replication occurs faster than predicted by population measurements...
November 7, 2017: Cell Reports
https://www.readbyqxmd.com/read/29116458/3d-gut-liver-chip-with-a-pk-model-for-prediction-of-first-pass-metabolism
#20
Dong Wook Lee, Sang Keun Ha, Inwook Choi, Jong Hwan Sung
Accurate prediction of first-pass metabolism is essential for improving the time and cost efficiency of drug development process. Here, we have developed a microfluidic gut-liver co-culture chip that aims to reproduce the first-pass metabolism of oral drugs. This chip consists of two separate layers for gut (Caco-2) and liver (HepG2) cell lines, where cells can be co-cultured in both 2D and 3D forms. Both cell lines were maintained well in the chip, verified by confocal microscopy and measurement of hepatic enzyme activity...
November 7, 2017: Biomedical Microdevices
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