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https://www.readbyqxmd.com/read/28346363/rapid-and-low-cost-crp-measurement-by-integrating-a-paper-based-microfluidic-immunoassay-with-smartphone-crp-chip
#1
Meili Dong, Jiandong Wu, Zimin Ma, Hagit Peretz-Soroka, Michael Zhang, Paul Komenda, Navdeep Tangri, Yong Liu, Claudio Rigatto, Francis Lin
Traditional diagnostic tests for chronic diseases are expensive and require a specialized laboratory, therefore limiting their use for point-of-care (PoC) testing. To address this gap, we developed a method for rapid and low-cost C-reactive protein (CRP) detection from blood by integrating a paper-based microfluidic immunoassay with a smartphone (CRP-Chip). We chose CRP for this initial development because it is a strong biomarker of prognosis in chronic heart and kidney disease. The microfluidic immunoassay is realized by lateral flow and gold nanoparticle-based colorimetric detection of the target protein...
March 26, 2017: Sensors
https://www.readbyqxmd.com/read/28345085/formation-of-surface-nanodroplets-facing-a-structured-microchannel-wall
#2
Haitao Yu, Shantanu Maheshwari, Jiuyang Zhu, Detlef Lohse, Xuehua Zhang
Surface nanodroplets are important units for lab-on-a-chip devices, compartmentalised catalytic reactions, high-resolution near-field imaging, and many others. Solvent exchange is a simple solution-based bottom-up approach for producing surface nanodroplets by displacing a good solvent of the droplet liquid by a poor one in a narrow channel in the laminar regime. The droplet size is controlled by the solution composition and the flow conditions during the solvent exchange. In this paper, we investigated the effects of local microfluidic structures on the formation of surface nanodroplets...
March 27, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28345028/self-powered-integrated-microfluidic-point-of-care-low-cost-enabling-simple-chip
#3
Erh-Chia Yeh, Chi-Cheng Fu, Lucy Hu, Rohan Thakur, Jeffrey Feng, Luke P Lee
Portable, low-cost, and quantitative nucleic acid detection is desirable for point-of-care diagnostics; however, current polymerase chain reaction testing often requires time-consuming multiple steps and costly equipment. We report an integrated microfluidic diagnostic device capable of on-site quantitative nucleic acid detection directly from the blood without separate sample preparation steps. First, we prepatterned the amplification initiator [magnesium acetate (MgOAc)] on the chip to enable digital nucleic acid amplification...
March 2017: Science Advances
https://www.readbyqxmd.com/read/28344726/evaluation-of-disposable-microfluidic-chip-design-for-automated-and-fast-immunoassays
#4
Guochun Wang, Champak Das, Bradley Ledden, Qian Sun, Chien Nguyen, Sai Kumar
We report here, the design and development of a disposable immunoassay chip for protein biomarker detection within ∼1 h. The unique design allows for real-time dynamic calibration of immunoassay for multiple biomarker detections on the chip. The limit of detection achieved for this test chip is 10 pg/ml for IL6, and 50 pg/ml for GFAP with a detection time of 1 h. The prototype instrument used for flowing the reagents through the chip can be easily assembled from off-the-shelf components with the final chemiluminescent detection carried out in a commercial plate reader...
January 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28343437/organs-on-chips-progress-challenges-and-future-directions
#5
Lucie A Low, Danilo A Tagle
The National Institutes of Health Microphysiological Systems (MPS) program, led by the National Center for Advancing Translational Sciences, is part of a joint effort on MPS development with the Defense Advanced Research Projects Agency and with regulatory guidance from FDA, is now in its final year of funding. The program has produced many tangible outcomes in tissue chip development in terms of stem cell differentiation, microfluidic engineering, platform development, and single and multi-organ systems-and continues to help facilitate the acceptance and use of tissue chips by the wider community...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28342274/a-feasibility-study-for-enrichment-of-highly-aggressive-cancer-subpopulations-by-their-biophysical-properties-via-dielectrophoresis-enhanced-with-synergistic-fluid-flow
#6
Temple Anne Douglas, Jaka Cemazar, Nikita Balani, Daniel C Sweeney, Eva M Schmelz, Rafael V Davalos
A common problem with cancer treatment is the development of treatment resistance and tumor recurrence that result from treatments that kill most tumor cells yet leave behind aggressive cells to repopulate. Presented here is a microfluidic device that can be used to isolate tumor subpopulations to optimize treatment selection. Dielectrophoresis (DEP) is a phenomenon where particles are polarized by an electric field and move along the electric field gradient. Different cell subpopulations have different DEP responses depending on their bioelectrical phenotype, which, we hypothesize, correlate with aggressiveness...
March 25, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28340791/solid-phase-extraction-microfluidic-devices-for-matrix-removal-in-trace-element-assay-of-actinide-materials
#7
Jun Gao, Benjamin T Manard, Alonso Castro, Dennis P Montoya, Ning Xu, Rebecca M Chamberlin
Advances in sample nebulization and injection technology have significantly reduced the volume of solution required for trace impurity analysis in plutonium and uranium materials. Correspondingly, we have designed and tested a novel chip-based microfluidic platform, containing a 100-µL or 20-µL solid-phase microextraction column, packed by centrifugation, which supports nuclear material mass and solution volume reductions of 90% or more compared to standard methods. Quantitative recovery of 28 trace elements in uranium was demonstrated using a UTEVA chromatographic resin column, and trace element recovery from thorium (a surrogate for plutonium) was similarly demonstrated using anion exchange resin AG MP-1...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28338083/microvasculature-on-a-chip-study-of-the-endothelial-surface-layer-and-the-flow-structure-of-red-blood-cells
#8
Daria Tsvirkun, Alexei Grichine, Alain Duperray, Chaouqi Misbah, Lionel Bureau
Microvasculatures-on-a-chip, i.e. in vitro models that mimic important features of microvessel networks, have gained increasing interest in recent years. Such devices have allowed investigating pathophysiological situations involving abnormal biophysical interactions between blood cells and vessel walls. Still, a central question remains regarding the presence, in such biomimetic systems, of the endothelial glycocalyx. The latter is a glycosaminoglycans-rich surface layer exposed to blood flow, which plays a crucial role in regulating the interactions between circulating cells and the endothelium...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28335593/simultaneous-single-cell-analysis-of-na-k-ca2-and-mg2-in-neuron-like-pc-12-cells-in-a-microfluidic-system
#9
Lu Li, Yuanyuan Fan, Qingling Li, Renjie Sheng, Haibin Si, Juan Fang, Lili Tong, Bo Tang
Various intracellular metal ions have closely related functional roles in the nervous system. An excess or deficiency of essential metal ions can contribute to neurodegenerative diseases. Thus, the detection of various metal ions in neurons is important for diagnosing and monitoring these diseases. In particular, single-cell analysis of multiple metal ions allows us to not only understand the cellular heterogeneity and differentiation, but also determine the actual relationships among multiple metal ions in each individual cell...
March 24, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28334623/rapid-on-chip-apoptosis-assay-on-human-carcinoma-cells-based-on-annexin-v-quantum-dot-probes
#10
Helena Montón, Mariana Medina-Sánchez, Joan Antoni Soler, Andrzej Chałupniak, Carme Nogués, Arben Merkoçi
Despite all the efforts made over years to study the cancer expression and the metastasis event, there is not a clear understanding of its origins and effective treatment. Therefore, more specialized and rapid techniques are required for studying cell behaviour under different drug-based treatments. Here we present a quantum dot signalling-based cell assay carried out in a segmental microfluidic device that allows studying the effect of anti-cancer drugs in cultured cell lines by monitoring phosphatidylserine translocation that occurs in early apoptosis...
March 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28324958/ultrafast-oscillatory-flow-pcr-for-amplication-of-urine-short-tandem-repeats
#11
Haoyu Tang, Tao Dong
The short tandem repeats (STRs) in human urine which is the most sensitive body fluid can be serviced as biomarkers for the individual identification. PCR microchips are automated, sensitive and rapid. In this report, COMSOL with Finite Element Analysis (FEA) is employed to study the temperature distribution of the base layer to optimize the structure of the microfluidic chip. Meanwhile, the oscillatory PCR microdevice is preliminary tested by DNA samples extracted from urine with the FTA papers and CSF1PO, TPOX, TH01 Multiplex kits...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28322517/materials-for-microfluidic-immunoassays-a-review
#12
REVIEW
Lei Mou, Xingyu Jiang
Conventional immunoassays suffer from at least one of these following limitations: long processing time, high costs, poor user-friendliness, technical complexity, poor sensitivity and specificity. Microfluidics, a technology characterized by the engineered manipulation of fluids in channels with characteristic lengthscale of tens of micrometers, has shown considerable promise for improving immunoassays that could overcome these limitations in medical diagnostics and biology research. The combination of microfluidics and immunoassay can detect biomarkers with faster assay time, reduced volumes of reagents, lower power requirements, and higher levels of integration and automation compared to traditional approaches...
March 21, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28322404/device-and-programming-abstractions-for-spatiotemporal-control-of-active-micro-particle-swarms
#13
Amy T Lam, Karina G Samuel-Gama, Jonathan Griffin, Matthew Loeun, Lukas C Gerber, Zahid Hossain, Nate J Cira, Seung Ah Lee, Ingmar H Riedel-Kruse
We present a hardware setup and a set of executable commands for spatiotemporal programming and interactive control of a swarm of self-propelled microscopic agents inside a microfluidic chip. In particular, local and global spatiotemporal light stimuli are used to direct the motion of ensembles of Euglena gracilis, a unicellular phototactic organism. We develop three levels of programming abstractions (stimulus space, swarm space, and system space) to create a scripting language for directing swarms. We then implement a multi-level proof-of-concept biotic game using these commands to demonstrate their utility...
March 21, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28322047/thiol-ene-monolithic-pepsin-microreactor-with-a-3d-printed-interface-for-efficient-uplc-ms-peptide-mapping-analyses
#14
Alexander Jönsson, Rasmus R Svejdal, Nanna B Bøgelund, Tam T T N Nguyen, Henrik Flindt, Jörg P Kutter, Kasper D Rand, Josiane Penelope Lafleur
To improve the sample handling, and reduce cost and preparation time, of peptide mapping LC-MS workflows in protein analytical research, we here investigate the possibility of replacing conventional enzymatic digestion methods with a polymer microfluidic chip-based enzyme reactor. Off-stoichiometric thiol-ene is utilized as both bulk material and as a monolithic stationary phase for immobilization of the proteolytic enzyme pepsin. The digestion efficiency of the, thiol-ene based, immobilized enzym reactor (IMER) is compared to that of a conventional, agarose packed bed, pepsin IMER column commonly used in LC-MS-based protein analyses...
March 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28319898/immunodetection-of-salivary-biomarkers-by-an-optical-microfluidic-biosensor-with-polyethylenimine-modified-polythiophene-c70-organic-photodetectors
#15
Tao Dong, Nuno Miguel Matos Pires
This work reports a novel optical microfluidic biosensor with highly sensitive organic photodetectors (OPDs) for absorbance-based detection of salivary protein biomarkers at the point of care. The compact and miniaturized biosensor has comprised OPDs made of polythiophene-C70 bulk heterojunction for the photoactive layer; whilst a calcium-free cathode interfacial layer, made of linear polyethylenimine, was incorporated to the photodetectors to enhance the low cost. The OPDs realized onto a glass chip were aligned to antibody-functionalized chambers of a poly(methyl methacrylate) microfluidic chip, in where immunogold-silver assays were conducted...
March 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28319082/passive-phloem-loading-and-long-distance-transport-in-a-synthetic-tree-on-a-chip
#16
Jean Comtet, Kaare H Jensen, Robert Turgeon, Abraham D Stroock, A E Hosoi
Vascular plants rely on differences in osmotic pressure to export sugars from regions of synthesis (mature leaves) to sugar sinks (roots, fruits). In this process, known as Münch pressure flow, the loading of sugars from photosynthetic cells to the export conduit (the phloem) is crucial, as it sets the pressure head necessary to power long-distance transport. Whereas most herbaceous plants use active mechanisms to increase phloem sugar concentration above that of the photosynthetic cells, in most tree species, for which transport distances are largest, loading seems, counterintuitively, to occur by means of passive symplastic diffusion from the mesophyll to the phloem...
March 20, 2017: Nature Plants
https://www.readbyqxmd.com/read/28318376/advances-in-on-chip-vascularization
#17
Kristina Haase, Roger D Kamm
Microfluidics is invaluable for studying microvasculature, development of organ-on-chip models and engineering microtissues. Microfluidic design can cleverly control geometry, biochemical gradients and mechanical stimuli, such as shear and interstitial flow, to more closely mimic in vivo conditions. In vitro vascular networks are generated by two distinct approaches: via endothelial-lined patterned channels, or by self-assembled networks. Each system has its own benefits and is amenable to the study of angiogenesis, vasculogenesis and cancer metastasis...
March 20, 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/28317863/dynamic-monitoring-of-membrane-nanotubes-formation-induced-by-vaccinia-virus-on-a-high-throughput-microfluidic-chip
#18
Min Xiao, Na Xu, Cheng Wang, Dai-Wen Pang, Zhi-Ling Zhang
Membrane nanotubes (MNTs) are physical connections for intercellular communication and induced by various viruses. However, the formation of vaccinia virus (VACV)-induced MNTs has never been studied. In this report, VACV-induced MNTs formation process was monitored on a microfluidic chip equipped with a series of side chambers, which protected MNTs from fluidic shear stress. MNTs were formed between susceptible cells and be facilitated by VACV infection through three patterns. The formed MNTs varied with cell migration and virus concentration...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28317836/microscale-symmetrical-electroporator-array-as-a-versatile-molecular-delivery-system
#19
Mengxing Ouyang, Winfield Hill, Jung Hyun Lee, Soojung Claire Hur
Successful developments of new therapeutic strategies often rely on the ability to deliver exogenous molecules into cytosol. We have developed a versatile on-chip vortex-assisted electroporation system, engineered to conduct sequential intracellular delivery of multiple molecules into various cell types at low voltage in a dosage-controlled manner. Micro-patterned planar electrodes permit substantial reduction in operational voltages and seamless integration with an existing microfluidic technology. Equipped with real-time process visualization functionality, the system enables on-chip optimization of electroporation parameters for cells with varying properties...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28317005/a-cost-effective-micromilling-platform-for-rapid-prototyping-of-microdevices
#20
Daniel P Yen, Yuta Ando, Keyue Shen
Micromilling has great potential in producing microdevices for lab-on-a-chip and organ-on-a-chip applications, but has remained under-utilized due to the high machinery costs and limited accessibility. In this paper, we assessed the machining capabilities of a low-cost 3-D mill in polycarbonate material, which were showcased by the production of microfluidic devices. The study demonstrates that this particular mill is well suited for the fabrication of multi-scale microdevices with feature sizes from micrometers to centimeters...
December 2016: Technology
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