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https://www.readbyqxmd.com/read/28736788/characterisation-of-anticancer-peptides-at-the-single-cell-level
#1
L Armbrecht, G Gabernet, F Kurth, J A Hiss, G Schneider, P S Dittrich
The development of efficacious anticancer therapeutics is difficult due to the heterogeneity of the cellular response to chemotherapy. Anticancer peptides (ACPs) are promising drug candidates that have been shown to be active against a range of cancer cells. However, few ACP studies focus on tumour single-cell heterogeneities. In order to address this need, we developed a microfluidic device and an imaging procedure that enable the capture, monitoring, and analysis of several hundred single cells for the study of drug response...
July 24, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28735045/multiplexed-capillary-microfluidic-immunoassay-with-smartphone-data-acquisition-for-parallel-mycotoxin-detection
#2
Jessica M D Machado, Ruben R G Soares, Virginia Chu, João P Conde
The field of microfluidics holds great promise for the development of simple and portable lab-on-a-chip systems. The use of capillarity as a means of fluidic manipulation in lab-on-a-chip systems can potentially reduce the complexity of the instrumentation and allow the development of user-friendly devices for point-of-need analyses. In this work, a PDMS microchannel-based, colorimetric, autonomous capillary chip provides a multiplexed and semi-quantitative immunodetection assay. Results are acquired using a standard smartphone camera and analyzed with a simple gray scale quantification procedure...
July 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28734608/sequence-specific-dna-solid-phase-extraction-in-an-on-chip-monolith-towards-detection-of-antibiotic-resistance-genes
#3
Radim Knob, Daniel B Nelson, Richard A Robison, Adam T Woolley
Antibiotic resistance of bacteria is a growing problem and presents a challenge for prompt treatment in patients with sepsis. Currently used methods rely on culturing or amplification; however, these steps are either time consuming or suffer from interference issues. A microfluidic device was made from black polypropylene, with a monolithic column modified with a capture oligonucleotide for sequence selective solid-phase extraction of a complementary target from a lysate sample. Porous properties of the monolith allow flow and hybridization of a target complementary to the probe immobilized on the column surface...
July 10, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28734352/real-time-observation-of-perturbation-of-a-drosophila-embryo-s-early-cleavage-cycles-with-microfluidics
#4
Zeqing Bai, Hongcun Bao, Yingying Yuan, Xiaohang Yang, Yongmei Xi, Min Wang
It is of great importance to understand biochemical system's behavior toward environmental perturbation during the development of living organisms. Here a microfluidic platform for Drosophila embryo's online development and observation is presented. The system is capable of developing the embryo's anterior and posterior halves controlled at different temperature environments, and it can be easily coupled with a confocal microscope for real-time image acquisition. The microfluidic chip is consisted of a polymethylmethacrylate (PMMA) substrate with a thickness of 4...
August 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28726375/nanoscale-hydrodynamic-film-for-diffusive-mass-transport-control-in-compartmentalized-microfluidic-chambers
#5
Minseok Kim, Ji Won Lim, Sung Kuk Lee, Taesung Kim
A compartmentalized microfluidic chamber array that not only offers separate cell culture environments but also independent control of the diffusion of small molecules provides an extremely useful platform for cell cultivations and versatile cellular assays. However, it is challenging to incorporate both cell compartmentalization and active diffusion control in real-time and precise manners. Here, we present a novel nanoscale hydrodynamic film (NHF) that is formed between a solid substrate and a polydimethylsiloxane (PDMS) surface...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28723908/sorting-cells-by-their-density
#6
Nazila Norouzi, Heran C Bhakta, William H Grover
Sorting cells by their type is an important capability in biological research and medical diagnostics. However, most cell sorting techniques rely on labels or tags, which may have limited availability and specificity. Sorting different cell types by their different physical properties is an attractive alternative to labels because all cells intrinsically have these physical properties. But some physical properties, like cell size, vary significantly from cell to cell within a cell type; this makes it difficult to identify and sort cells based on their sizes alone...
2017: PloS One
https://www.readbyqxmd.com/read/28723147/quantum-dot-based-molecularly-imprinted-polymers-on-three-dimensional-origami-paper-microfluidic-chip-for-fluorescence-detection-of-phycocyanin
#7
Bowei Li, Zhong Zhang, Ji Qi, Na Zhou, Song Qin, Jaebum Choo, Lingxin Chen
In this work, we developed a novel strategy using fluorescent quantum dots (QDs) combined with molecularly imprinted polymers (MIPs) on three-dimensional (3D) origami paper-based microfluidic devices for specific recognition and sensitive detection of phycocyanin. This method can realize the liquid phase of QDs@MIPs being transferred to the solid-phase paper base and achieve easy portability for the analysis. Under optimal conditions, we successfully demonstrated the proposed paper@QDs@MIPs 3D microfluidic chip for the sensitive and selective detection of phycocyanin protein target in a simple and robust manner...
February 24, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28723125/pharmacokinetics-on-a-chip-using-label-free-sers-technique-for-programmable-dual-drug-analysis
#8
Jiayuan Fei, Lei Wu, Yizhi Zhang, Shenfei Zong, Zhuyuan Wang, Yiping Cui
Synergistic effects of dual or multiple drugs have attracted great attention in medical fields, especially in cancer therapies. We provide a programmable microfluidic platform for pharmacokinetic detection of multiple drugs in multiple cells. The well-designed microfluidic platform includes two 2 × 3 microarrays of cell chambers, two gradient generators, and several pneumatic valves. Through the combined use of valves and gradient generators, each chamber can be controlled to infuse different kinds of living cells and drugs with specific concentrations as needed...
June 23, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28722238/the-preparation-of-microfluidic-architecture-with-monolithic-materials-using-a-dual-porous-silica-structure
#9
Christopher Birch, Mohammad Mehdi Nasr Esfahani, Kirsty J Shaw, Cordula Kemp, Stephen James Haswell, Charlotte Dyer
A microfluidic device (MD) has been developed which features a porous silica (PS) monolithic disk synthesized from tetramethyl orthosilicate (TMOS), incorporated into the device post-fabrication and sealed in place with a second PS monolithic layer, synthesized from potassium silicate. This dual porous silica (DPS) structure provides a pathway for sample introduction to the microfluidic device and offers an ideal platform for solid phase extraction (SPE) methodologies which can be rapidly and efficiently integrated into a chip-based format...
July 19, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28722051/holographic-microscope-slide-in-a-spatio-temporal-imaging-modality-for-reliable-3d-cell-counting
#10
Biagio Mandracchia, Vittorio Bianco, Zhe Wang, Martina Mugnano, Alessia Bramanti, Melania Paturzo, Pietro Ferraro
In the current trend of miniaturization and simplification of imaging flow cytometry, Lab-on-a-Chip (LoC) microfluidic devices represent an innovative and cost-effective solution. In this framework, we propose for the first time a novel platform based on the compactness of a holographic microscope slide (HMS) in combination with the new computational features of space-time digital holography (STDH) that uses a 1D linear sensor array (LSA) instead of 2D CCD or CMOS cameras to respond to real diagnostic needs...
July 19, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28721842/rapid-seafood-species-identification-using-chip-based-capillary-electrophoresis-and-protein-pattern-matching
#11
Calvin Walker, Cheryl Lassitter, Shannara Lynn, Courtney Ford, Kevin Rademacher, Angela Ruple, Jon Bell
Authenticity is crucial to the seafood industry, as substitution and mislabeling have important economic, environmental, and food safety consequences. To address this problem, protein profiling and software algorithm techniques were developed to classify fish muscle samples by species. The method uses water-based protein extraction, chip-based microfluidic electrophoresis (Agilent 2100 Bioanalyzer) for the analysis of high abundance fish muscle proteins, and a novel data analysis method for species-specific protein pattern recognition...
July 19, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28719742/integrated-smartphone-app-chip-system-for-on-site-ppb-level-colorimetric-quantitation-of-aflatoxins
#12
Xiaochun Li, Fan Yang, Jessica X H Wong, Hua-Zhong Yu
We demonstrate herein an integrated, smartphone-app-chip (SPAC) system for on-site quantitation of food toxins, as demonstrated with aflatoxin B1 (AFB1), at parts-per-billion (ppb) level in food products. The detection is based on an indirect competitive immunoassay fabricated on a transparent plastic chip with the assistance of a microfluidic channel plate. A 3D-printed optical accessory attached to a smartphone is adapted to align the assay chip and to provide uniform illumination for imaging, with which high-quality images of the assay chip are captured by the smartphone camera and directly processed using a custom-developed Android app...
July 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28718979/in-situ-two-step-photoreduced-sers-materials-for-on-chip-single-molecule-spectroscopy-with-high-reproducibility
#13
Wenjie Yan, Longkun Yang, Jianing Chen, Yaqi Wu, Peijie Wang, Zhipeng Li
A method is developed to synthesize surface-enhanced Raman scattering (SERS) materials capable of single-molecule detection, integrated with a microfluidic system. Using a focused laser, silver nanoparticle aggregates as SERS monitors are fabricated in a microfluidic channel through photochemical reduction. After washing out the monitor, the aggregates are irradiated again by the same laser. This key step leads to full reduction of the residual reactants, which generates numerous small silver nanoparticles on the former nanoaggregates...
July 18, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28717801/mimicking-arterial-thrombosis-in-a-3d-printed-microfluidic-in-vitro-vascular-model-based-on-computed-tomography-angiography-data
#14
Pedro F Costa, Hugo J Albers, John E A Linssen, Heleen H T Middelkamp, Linda van der Hout, Robert Passier, Albert van den Berg, Jos Malda, Andries D van der Meer
Arterial thrombosis is the main instigating factor of heart attacks and strokes, which result in over 14 million deaths worldwide every year. The mechanism of thrombosis involves factors from the blood and the vessel wall, and it also relies strongly on 3D vessel geometry and local blood flow patterns. Microfluidic chip-based vascular models allow controlled in vitro studies of the interaction between vessel wall and blood in thrombosis, but until now, they could not fully recapitulate the 3D geometry and blood flow patterns of real-life healthy or diseased arteries...
July 18, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28717569/histogram-analysis-for-smartphone-based-rapid-hematocrit-determination
#15
Uddin M Jalal, Sang C Kim, Joon S Shim
A novel and rapid analysis technique using histogram has been proposed for the colorimetric quantification of blood hematocrits. A smartphone-based "Histogram" app for the detection of hematocrits has been developed integrating the smartphone embedded camera with a microfluidic chip via a custom-made optical platform. The developed histogram analysis shows its effectiveness in the automatic detection of sample channel including auto-calibration and can analyze the single-channel as well as multi-channel images...
July 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28714133/tilted-pillar-array-fabrication-by-the-combination-of-proton-beam-writing-and-soft-lithography-for-microfluidic-cell-capture-part-2-image-sequence-analysis-based-evaluation-and-biological-application
#16
Gabor Járvás, Tamas Varga, Marton G Szigeti, Laszlo Hajba, Peter Fürjes, Istvan Rajta, Andras Guttman
As a continuation of our previously published work, this paper presents a detailed evaluation of a microfabricated cell capture device utilizing a doubly tilted micropillar array. The device was fabricated using a novel hybrid technology based on the combination of proton beam writing and conventional lithography techniques. Tilted pillars offer unique flow characteristics and support enhanced fluidic interaction for improved immuno-affinity based cell capture. The performance of the microdevice was evaluated by an image sequence analysis based in-house developed single cell tracking system...
July 17, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28713851/building-an-experimental-model-of-the-human-body-with-non-physiological-parameters
#17
Joseph M Labuz, Christopher Moraes, David R Mertz, Brendan M Leung, Shuichi Takayama
New advances in engineering and biomedical technology have enabled recent efforts to capture essential aspects of human physiology in microscale, in-vitro systems. The application of these advances to experimentally model complex processes in an integrated platform - commonly called a 'human-on-a-chip (HOC)' - requires that relevant compartments and parameters be sized correctly relative to each other and to the system as a whole. Empirical observation, theoretical treatments of resource distribution systems and natural experiments can all be used to inform rational design of such a system, but technical and fundamental challenges (e...
March 2017: Technology
https://www.readbyqxmd.com/read/28713479/automated-selection-of-aptamers-against-cholangiocarcinoma-cells-on-an-integrated-microfluidic-platform
#18
Priya Gopinathan, Lien-Yu Hung, Chih-Hung Wang, Nai-Jung Chiang, Yu-Chun Wang, Yan-Shen Shan, Gwo-Bin Lee
An integrated microfluidic system capable of automatically identifying aptamers specific to cholangiocarcinoma (CCA) cells was developed herein. The developed system was capable of performing cell-based systematic evolution of ligands via an exponential enrichment (Cell-SELEX) process on-chip, and only six rounds of Cell-SELEX were required to identify high specificity aptamers; this represents a significant improvement in speed over conventional SELEX, in which 15-20 rounds are typically required. Using the microfluidic chip developed, three aptamers specific to CCA cells (one for SNU-478 cells and two for HuCCT-1 cells) were successfully screened...
July 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28713478/an-integrated-microfluidic-system-for-the-isolation-and-detection-of-ovarian-circulating-tumor-cells-using-cell-selection-and-enrichment-methods
#19
Sung-Chi Tsai, Lien-Yu Hung, Gwo-Bin Lee
Gynecological cancer is difficult to be diagnosed at early stages. The relatively high mortality rate has been a serious issue accordingly. We herein reported a diagnosis method by using circulating tumor cells (CTCs) which have been extensively explored as a potential tool for diagnostics and prognostics of ovarian cancers. Nonetheless, the detection of CTCs still remains a challenge because of the difficulty in isolating them from whole blood samples since they are shed into the vasculature from primary tumors and circulate irregularly in the bloodstream in extremely low concentrations...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28713477/mopsa-a-microfluidics-optimized-particle-simulation-algorithm
#20
Junchao Wang, Victor G J Rodgers, Philip Brisk, William H Grover
Computer simulation plays a growing role in the design of microfluidic chips. However, the particle tracers in some existing commercial computational fluid dynamics software are not well suited for accurately simulating the trajectories of particles such as cells, microbeads, and droplets in microfluidic systems. To address this issue, we present a microfluidics-optimized particle simulation algorithm (MOPSA) that simulates the trajectories of cells, droplets, and other particles in microfluidic chips with more lifelike results than particle tracers in existing commercial software...
May 2017: Biomicrofluidics
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