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https://www.readbyqxmd.com/read/29770425/dynamic-microfluidic-nanocalorimetry-system-for-measuring-caenorhabditis-elegans-metabolic-heat
#1
Roger Krenger, Thomas Lehnert, Martin A M Gijs
Basal heat production is a key phenotype for assessing the metabolic activity of small living organisms. Here, we present a new nanocalorimetric system, based on thin film thermopile sensors combined with microfluidic chips for measuring metabolic heat signals generated by Caenorhabditis elegans larval populations (60 to 220 organisms). In addition to versatile on-chip fluidic manipulation, our microfluidic approach allows confining worm populations close to the sensor surface, thus increasing the sensitivity of the assays...
May 17, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29767998/rapid-on-chip-assembly-of-niosomes-batch-versus-continuous-flow-reactors
#2
Sara Garcia-Salinas, Erico Himawan, Gracia Mendoza, Manuel Arruebo, Victor Sebastian Cabeza
The large-scale continuous production of niosomes remains challenging. The inherent drawbacks of batch processes such as large particle polydispersity and reduced batch-to-batch reproducibility are here overcome by using commercially available microfluidic reactors. Compared to the traditional batch-based film hydration method, herein, we demonstrate that it is possible to carry out the homogeneous, large-scale (up to 120mg/min) production of niosomes using two different synthesis techniques (the thin film hydration method and the emulsification technique)...
May 16, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29767364/single-cell-mrna-seq-using-the-fluidigm-c1-system-and-integrated-fluidics-circuits
#3
Haibiao Gong, Devin Do, Ramesh Ramakrishnan
Single-cell mRNA-seq is a valuable tool to dissect expression profiles and to understand the regulatory network of genes. Microfluidics is well suited for single-cell analysis owing both to the small volume of the reaction chambers and easiness of automation. Here we describe the workflow of single-cell mRNA-seq using C1 IFC, which can isolate and process up to 96 cells. Both on-chip procedure (lysis, reverse transcription, and preamplification PCR) and off-chip sequencing library preparation protocols are described...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29765657/facile-fabrication-of-microfluidic-surface-enhanced-raman-scattering-devices-via-lift-up-lithography
#4
Yuanzi Wu, Ye Jiang, Xiaoshan Zheng, Shasha Jia, Zhi Zhu, Bin Ren, Hongwei Ma
We describe a facile and low-cost approach for a flexibly integrated surface-enhanced Raman scattering (SERS) substrate in microfluidic chips. Briefly, a SERS substrate was fabricated by the electrostatic assembling of gold nanoparticles, and shaped into designed patterns by subsequent lift-up soft lithography. The SERS micro-pattern could be further integrated within microfluidic channels conveniently. The resulting microfluidic SERS chip allowed ultrasensitive in situ SERS monitoring from the transparent glass window...
April 2018: Royal Society Open Science
https://www.readbyqxmd.com/read/29757285/microfluidic-chips-for-in-situ-crystal-x-ray-diffraction-and-in-situ-dynamic-light-scattering-for-serial-crystallography
#5
Yannig Gicquel, Robin Schubert, Svetlana Kapis, Gleb Bourenkov, Thomas Schneider, Markus Perbandt, Christian Betzel, Henry N Chapman, Michael Heymann
This protocol describes fabricating microfluidic devices with low X-ray background optimized for goniometer based fixed target serial crystallography. The devices are patterned from epoxy glue using soft lithography and are suitable for in situ X-ray diffraction experiments at room temperature. The sample wells are lidded on both sides with polymeric polyimide foil windows that allow diffraction data collection with low X-ray background. This fabrication method is undemanding and inexpensive. After the sourcing of a SU-8 master wafer, all fabrication can be completed outside of a cleanroom in a typical research lab environment...
April 24, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29756630/a-biomimetic-microfluidic-model-to-study-signalling-between-endothelial-and-vascular-smooth-muscle-cells-under-hemodynamic-conditions
#6
Nicole C A van Engeland, Andreas M A O Pollet, Jaap M J den Toonder, Carlijn V C Bouten, Oscar M J A Stassen, Cecilia M Sahlgren
Cell signalling and mechanics influence vascular pathophysiology and there is an increasing demand for in vitro model systems that enable examination of signalling between vascular cells under hemodynamic conditions. Current 3D vessel wall constructs do not recapitulate the mechanical conditions of the native tissue nor do they allow examination of cell-cell interactions under relevant hemodynamic conditions. Here, we describe a 3D microfluidic chip model of arterial endothelial and smooth muscle cells where cellular organization, composition and interactions, as well as the mechanical environment of the arterial wall are mimicked...
May 14, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29755926/challenges-in-paper-based-fluorogenic-optical-sensing-with-smartphones
#7
REVIEW
Tiffany-Heather Ulep, Jeong-Yeol Yoon
Application of optically superior, tunable fluorescent nanotechnologies have long been demonstrated throughout many chemical and biological sensing applications. Combined with microfluidics technologies, i.e. on lab-on-a-chip platforms, such fluorescent nanotechnologies have often enabled extreme sensitivity, sometimes down to single molecule level. Within recent years there has been a peak interest in translating fluorescent nanotechnology onto paper-based platforms for chemical and biological sensing, as a simple, low-cost, disposable alternative to conventional silicone-based microfluidic substrates...
2018: Nano Convergence
https://www.readbyqxmd.com/read/29755636/microfluidic-chip-for-non-invasive-analysis-of-tumor-cells-interaction-with-anti-cancer-drug-doxorubicin-by-afm-and-raman-spectroscopy
#8
Han Zhang, Lifu Xiao, Qifei Li, Xiaojun Qi, Anhong Zhou
Raman spectroscopy has been playing an increasingly significant role for cell classification. Here, we introduce a novel microfluidic chip for non-invasive Raman cell natural fingerprint collection. Traditional Raman spectroscopy measurement of the cells grown in a Polydimethylsiloxane (PDMS) based microfluidic device suffers from the background noise from the substrate materials of PDMS when intended to apply as an in vitro cell assay. To overcome this disadvantage, the current device is designed with a middle layer of PDMS layer sandwiched by two MgF2 slides which minimize the PDMS background signal in Raman measurement...
March 2018: Biomicrofluidics
https://www.readbyqxmd.com/read/29755635/microfluidic-platform-for-probing-cancer-cells-migration-property-under-periodic-mechanical-confinement
#9
Dongce Ma, Ran Wang, Shuxun Chen, Tao Luo, Yu-Ting Chow, Dong Sun
Cancer cell migration and invasion, which are involved in tumour metastasis, are hard to predict and control. Numerous studies have demonstrated that physical cues influence cancer cell migration and affect tumour metastasis. In this study, we proposed the use of a microchannel chip equipped with a number of vertical constrictions to produce periodic compression forces on cells passing through narrow channels. The chip with repeated vertical confinement was applied on adherent MHCC-97L liver cancer cells and suspended OCI-AML leukaemia cells to determine the migration ability of these cancer cells...
March 2018: Biomicrofluidics
https://www.readbyqxmd.com/read/29755444/microfluidic-pcr-amplification-and-miseq-amplicon-sequencing-techniques-for-high-throughput-detection-and-genotyping-of-human-pathogenic-rna-viruses-in-human-feces-sewage-and-oysters
#10
Mamoru Oshiki, Takayuki Miura, Shinobu Kazama, Takahiro Segawa, Satoshi Ishii, Masashi Hatamoto, Takashi Yamaguchi, Kengo Kubota, Akinori Iguchi, Tadashi Tagawa, Tsutomu Okubo, Shigeki Uemura, Hideki Harada, Naohiro Kobayashi, Nobuo Araki, Daisuke Sano
Detection and genotyping of pathogenic RNA viruses in human and environmental samples are useful for monitoring the circulation and prevalence of these pathogens, whereas a conventional PCR assay followed by Sanger sequencing is time-consuming and laborious. The present study aimed to develop a high-throughput detection-and-genotyping tool for 11 human RNA viruses [Aichi virus; astrovirus; enterovirus; norovirus genogroup I (GI), GII, and GIV; hepatitis A virus; hepatitis E virus; rotavirus; sapovirus; and human parechovirus] using a microfluidic device and next-generation sequencer...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29755211/microfluidic-diatomite-analytical-devices-for-illicit-drug-sensing-with-ppb-level-sensitivity
#11
Xianming Kong, Xinyuan Chong, Kenny Squire, Alan X Wang
The escalating research interests in porous media microfluidics, such as microfluidic paper-based analytical devices, have fostered a new spectrum of biomedical devices for point-of-care (POC) diagnosis and biosensing. In this paper, we report microfluidic diatomite analytical devices (μDADs), which consist of highly porous photonic crystal biosilica channels, as an innovative lab-on-a-chip platform to detect illicit drugs. The μDADs in this work are fabricated by spin-coating and tape-stripping diatomaceous earth on regular glass slides with cross section of 400×30µm2 ...
April 15, 2018: Sensors and Actuators. B, Chemical
https://www.readbyqxmd.com/read/29752543/a-microfluidic-chip-containing-a-molecularly-imprinted-polymer-and-a-dna-aptamer-for-voltammetric-determination-of-carbofuran
#12
Shuhuai Li, Jianping Li, Jinhui Luo, Zhi Xu, Xionghui Ma
An electrochemical microfluidic chip is described for the determination of the insecticide carbofuran. It is making use of a molecularly imprinted film (MIP) and a DNA aptamer as dual recognition units. The analyte (carbofuran) is transported to the MIP and captured at the identification site in the channel. Then, carbofuran is eluted with carbinol-acetic acid and transported to the DNA aptamer on the testing position of the chip. It is captured again, this time by the aptamer, and detected by differential pulse voltammetry (DPV)...
May 11, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/29747161/a-3d-human-renal-cell-carcinoma-on-a-chip-for-the-study-of-tumor-angiogenesis
#13
Chris P Miller, Connor Tsuchida, Ying Zheng, Jonathan Himmelfarb, Shreeram Akilesh
Tractable human tissue-engineered 3D models of cancer that enable fine control of tumor growth, metabolism, and reciprocal interactions between different cell types in the tumor microenvironment promise to accelerate cancer research and pharmacologic testing. Progress to date mostly reflects the use of immortalized cancer cell lines, and progression to primary patient-derived tumor cells is needed to realize the full potential of these platforms. For the first time, we report endothelial sprouting induced by primary patient tumor cells in a 3D microfluidic system...
May 7, 2018: Neoplasia: An International Journal for Oncology Research
https://www.readbyqxmd.com/read/29746551/a-multi-chamber-microfluidic-intestinal-barrier-model-using-caco-2-cells-for-drug-transport-studies
#14
Hsih-Yin Tan, Sofie Trier, Ulrik L Rahbek, Martin Dufva, Jörg P Kutter, Thomas L Andresen
This paper presents the design and fabrication of a multi-layer and multi-chamber microchip system using thiol-ene 'click chemistry' aimed for drug transport studies across tissue barrier models. The fabrication process enables rapid prototyping of multi-layer microfluidic chips using different thiol-ene polymer mixtures, where porous Teflon membranes for cell monolayer growth were incorporated by masked sandwiching thiol-ene-based fluid layers. Electrodes for trans-epithelial electrical resistance (TEER) measurements were incorporated using low-melting soldering wires in combination with platinum wires, enabling parallel real-time monitoring of barrier integrity for the eight chambers...
2018: PloS One
https://www.readbyqxmd.com/read/29745611/-effect-of-cryoprotectant-removal-by-microfluidic-chip-on-developmental-capacity-of-oocytes
#15
Xingyue Yi, Xinli Zhou, Yun Yang, Jianjun Dai, Defu Zhang
In order to reduce osmotic damage and chemical toxicity of cryoprotectants (CPA) to oocytes during unloading process, the microfluidic chip was used to remove CPA from porcine MⅡ oocytes in this study. Firstly, the effects of unloading time, composition and concentration of diluting solutions of microfluidic method on survival rate and developmental capacity of oocytes were studied, then microfluidic method was compared with traditional one-step and two-step CPA unloading protocols. The results showed that when the total time is 8 minutes, the survival rate and morula rate of oocytes treated with microfluidic method could achieve 95...
February 1, 2018: Sheng Wu Yi Xue Gong Cheng Xue za Zhi, Journal of Biomedical Engineering, Shengwu Yixue Gongchengxue Zazhi
https://www.readbyqxmd.com/read/29745386/optical-calorimetry-in-microfluidic-droplets
#16
Jacob Chamoun, Ashish Pattekar, Farzaneh Afshinmanesh, Joerg Martini, Michael I Recht
A novel microfluidic calorimeter that measures the enthalpy change of reactions occurring in 100 μm diameter aqueous droplets in fluoropolymer oil has been developed. The aqueous reactants flow into a microfluidic droplet generation chip in separate fluidic channels, limiting contact between the streams until immediately before they form the droplet. The diffusion-driven mixing of reactants is predominantly restricted to within the droplet. The temperature change in droplets due to the heat of reaction is measured optically by recording the reflectance spectra of encapsulated thermochromic liquid crystals (TLC) that are added to one of the reactant streams...
May 10, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29743607/development-of-a-lab-on-a-chip-method-for-rapid-assay-of-xylella-fastidiosa-subsp-pauca-strain-codiro
#17
Maria Serena Chiriacò, Andrea Luvisi, Elisabetta Primiceri, Erika Sabella, Luigi De Bellis, Giuseppe Maruccio
Xylella fastidiosa subsp. pauca strain CoDiRO, a pathogen responsible for Olive Quick Decline Syndrome (OQDS), is strongly threatening the agricultural-based economy of South Italy and making its typical landscape collapse. The bacteria can also infect more than other twenty woody or shrub species and quarantine programs are carried out in Italy. Since symptoms of OQDS like leaf scorching and wilting of canopy may appear several months after infection and some hosts are asymptomatic, a tool for the rapid and early screening of plants is desirable, in order to plan a sudden control strategy and apply programs for pest management...
May 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29742104/3d-printed-microchannels-for-sub-nl-nmr-spectroscopy
#18
E Montinaro, M Grisi, M C Letizia, L Pethö, M A M Gijs, R Guidetti, J Michler, J Brugger, G Boero
Nuclear magnetic resonance (NMR) experiments on subnanoliter (sub-nL) volumes are hindered by the limited sensitivity of the detector and the difficulties in positioning and holding such small samples in proximity of the detector. In this work, we report on NMR experiments on liquid and biological entities immersed in liquids having volumes down to 100 pL. These measurements are enabled by the fabrication of high spatial resolution 3D printed microfluidic structures, specifically conceived to guide and confine sub-nL samples in the sub-nL most sensitive volume of a single-chip integrated NMR probe...
2018: PloS One
https://www.readbyqxmd.com/read/29741175/rapid-and-sensitive-detection-of-viral-nucleic-acids-using-silicon-microchips
#19
Laura Powell, Rodrigo Sergio Wiederkehr, Paige Damascus, Maarten Fauvart, Federico Buja, Tim Stakenborg, Stuart C Ray, Paolo Fiorini, William O Osburn
Clinical laboratory-based nucleic acid amplification tests (NAT) play an important role in diagnosing viral infections. However, laboratory infrastructure requirements and their failure to diagnose at the point-of-need (PON) limit their clinical utility in both resource-rich and -limited clinical settings. The development of fast and sensitive PON viral NAT may overcome these limitations. The scalability of silicon microchip manufacturing combined with advances in silicon microfluidics present an opportunity for development of rapid and sensitive PON NAT on silicon microchips...
May 9, 2018: Analyst
https://www.readbyqxmd.com/read/29740954/autonomous-magnetic-microrobots-by-navigating-gates-for-multiple-biomolecules-delivery
#20
Xinghao Hu, Byeonghwa Lim, Sri Ramulu Torati, Junjia Ding, Valentine Novosad, Mi-Young Im, Venu Reddy, Kunwoo Kim, Eunjoo Jung, Asif Iqbal Shawl, Eunjoo Kim, CheolGi Kim
The precise delivery of biofunctionalized matters is of great interest from the fundamental and applied viewpoints. In spite of significant progress achieved during the last decade, a parallel and automated isolation and manipulation of rare analyte, and their simultaneous on-chip separation and trapping, still remain challenging. Here, a universal micromagnet junction for self-navigating gates of microrobotic particles to deliver the biomolecules to specific sites using a remote magnetic field is described...
May 8, 2018: Small
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