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https://www.readbyqxmd.com/read/28213894/controllable-rotating-behavior-of-individual-dielectric-micro-rod-in-a-rotating-electric-field
#1
Weiyu Liu, Yukun Ren, Ye Tao, Yanbo Li, Xiaoming Chen
We report herein controllable rotating behavior of an individual dielectric micro-rod driven by a background rotating electric field. By disposing or removing structured floating microelectrode, the rigid rod suspended in electrolyte solution accordingly exhibits co-field or anti-field rotating motion. In the absence of the ideally polarizable metal surface, the dielectric rod rotates opposite to propagation of electric field, with the measured rotating rate much larger than predicted by Maxwell-Wager interfacial polarization theory incorporating surface conduction of fixed bond charge...
February 18, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28213275/a-single-microfluidic-chip-with-dual-surface-properties-for-protein-drug-delivery
#2
Mehrdad Bokharaei, Katayoun Saatchi, Urs O Häfeli
Principles of double emulsion generation were incorporated in a glass microfluidic chip fabricated with two different surface properties in order to produce protein loaded polymer microspheres. The microspheres were produced by integrating two microfluidic flow focusing systems and a multi-step droplet splitting and mixing system into one chip. The chip consists a hydrophobic and a hydrophilic section with two different heights, 12μm and 45μm, respectively. As a result, the protein is homogenously distributed throughout the polymer microsphere matrix, not just in its center (which has been studied before)...
February 14, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28213235/preparation-and-characterization-of-a-packed-bead-immobilized-trypsin-reactor-integrated-into-a-pdms-microfluidic-chip-for-rapid-protein-digestion
#3
Adam Kecskemeti, Attila Gaspar
This paper demonstrates the design, efficiency and applicability of a simple, inexpensive and high sample throughput microchip immobilized enzymatic reactor (IMER) for rapid protein digestion. The IMER contains conventional silica particles with covalently immobilized trypsin packed inside of a poly(dimethylsiloxane) (PDMS) microchip channel (10mm×1mm×35µm). The microchip consists of 9 different channels, enabling 9 simultaneous protein digestions. Trypsin was covalently immobilized using carbodiimide activation, the ideal trypsin/silica particle ratio (i...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28211938/establishment-and-validation-of-a-microfluidic-capillary-gel-electrophoresis-platform-method-for-purity-analysis-of-therapeutic-monoclonal-antibodies
#4
Michael T Smith, Shu Zhang, Troy Adams, Byron DiPaolo, Jennifer Dally
Capillary and microfluidic chip electrophoresis technologies are heavily utilized for development, characterization, release, and stability testing of biopharmaceuticals. Within the biopharmaceutical industry, CE-SDS and M-CGE are commonly used for purity determination by separation and quantitation of size-based variants. M-CGE is used primarily as a R&D tool for product and process development, while cGMP release and stability testing applications are commonly reserved for CE-SDS. This paper describes the establishment of a M-CGE platform method to be used for R&D and cGMP applications, including release and stability testing, for monoclonal antibodies...
February 17, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28211673/capillary-microfluidics-assembled-virus-like-particle-bio-nano-receptor-interfaces-for-label-free-biosensing
#5
Faheng Zang, Konstantinos Gerasopoulos, Adam D Brown, James N Culver, Reza Ghodssi
In this paper, a capillary microfluidics-integrated sensor system is developed for rapid assembly of bionanoreceptor interfaces on-chip and label-free biosensing. Genetically engineered Tobacco mosaic virus (TMV) virus-like particle (VLP), displaying thousands copies of identical receptor peptides FLAG-tags, are utilized as nanoceptors for antibody sensing. Controlled and accelerated assembly of VLP receptor layer on impedance sensor has been achieved using capillary effect and surface evaporation from an open-channel capillary microfluidic system...
February 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28211642/biomechanical-strain-exacerbates-inflammation-on-a-progeria-on-a-chip-model
#6
João Ribas, Yu Shrike Zhang, Patrícia R Pitrez, Jeroen Leijten, Mario Miscuglio, Jeroen Rouwkema, Mehmet Remzi Dokmeci, Xavier Nissan, Lino Ferreira, Ali Khademhosseini
Organ-on-a-chip platforms seek to recapitulate the complex microenvironment of human organs using miniaturized microfluidic devices. Besides modeling healthy organs, these devices have been used to model diseases, yielding new insights into pathophysiology. Hutchinson-Gilford progeria syndrome (HGPS) is a premature aging disease showing accelerated vascular aging, leading to the death of patients due to cardiovascular diseases. HGPS targets primarily vascular cells, which reside in mechanically active tissues...
February 17, 2017: Small
https://www.readbyqxmd.com/read/28198459/label-free-detection-of-influenza-viruses-using-a-reduced-graphene-oxide-based-electrochemical-immunosensor-integrated-with-a-microfluidic-platform
#7
Renu Singh, Seongkyeol Hong, Jaesung Jang
Reduced graphene oxide (RGO) has recently gained considerable attention for use in electrochemical biosensing applications due to its outstanding conducting properties and large surface area. This report presents a novel microfluidic chip integrated with an RGO-based electrochemical immunosensor for label-free detection of an influenza virus, H1N1. Three microelectrodes were fabricated on a glass substrate using the photolithographic technique, and the working electrode was functionalized using RGO and monoclonal antibodies specific to the virus...
February 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28198401/enrichment-of-circulating-head-and-neck-tumour-cells-using-spiral-microfluidic-technology
#8
Arutha Kulasinghe, Thao Huynh Phuoc Tran, Tony Blick, Ken O'Byrne, Erik W Thompson, Majid E Warkiani, Colleen Nelson, Liz Kenny, Chamindie Punyadeera
Whilst locoregional control of head and neck cancers (HNCs) has improved over the last four decades, long-term survival has remained largely unchanged. A possible reason for this is that the rate of distant metastasis has not changed. Such disseminated disease is reflected in measurable levels of cancer cells in the blood of HNC patients, referred to as circulating tumour cells (CTCs). Numerous marker-independent techniques have been developed for CTC isolation and detection. Recently, microfluidics-based platforms have come to the fore to avoid molecular bias...
February 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28197601/active-droplet-sorting-in-microfluidics-a-review
#9
REVIEW
Heng-Dong Xi, Hao Zheng, Wei Guo, Alfonso M Gañán-Calvo, Ye Ai, Chia-Wen Tsao, Jun Zhou, Weihua Li, Yanyi Huang, Nam-Trung Nguyen, Say Hwa Tan
The ability to manipulate and sort droplets is a fundamental issue in droplet-based microfluidics. Various lab-on-a-chip applications can only be realized if droplets are systematically categorized and sorted. These micron-sized droplets act as ideal reactors which compartmentalize different biological and chemical reagents. Array processing of these droplets hinges on the competence of the sorting and integration into the fluidic system. Recent technological advances only allow droplets to be actively sorted at the rate of kilohertz or less...
February 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28194934/continuous-flow-1-h-and-13-c-nmr-spectroscopy-in-microfluidic-stripline-nmr-chips
#10
Anna Jo Oosthoek-de Vries, Jacob Bart, Roald M Tiggelaar, Johannes W G Janssen, P Jan M van Bentum, Han J G E Gardeniers, Arno P M Kentgens
Microfluidic stripline NMR technology not only allows for NMR experiments to be performed on small sample volumes in the submicroliter range, but also experiments can easily be performed in continuous flow because of the stripline's favorable geometry. In this study we demonstrate the possibility of dual-channel operation of a microfluidic stripline NMR setup showing one- and two-dimensional (1)H, (13)C and heteronuclear NMR experiments under continuous flow. We performed experiments on ethyl crotonate and menthol, using three different types of NMR chips aiming for straightforward microfluidic connectivity...
February 6, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28194461/a-3d-microblade-structure-for-precise-and-parallel-droplet-splitting-on-digital-microfluidic-chips
#11
Cheng Dong, Yanwei Jia, Jie Gao, Tianlan Chen, Pui-In Mak, Mang-I Vai, Rui P Martins
Existing digital microfluidic (DMF) chips exploit the electrowetting on dielectric (EWOD) force to perform droplet splitting. However, the current splitting methods are not flexible and the volume of the droplets suffers from a large variation. Herein, we propose a DMF chip featuring a 3D microblade structure to enhance the droplet-splitting performance. By exploiting the EWOD force for shaping and manipulating the mother droplet, we obtain an average dividing error of <2% in the volume of the daughter droplets for a number of fluids such as deionized water, DNA solutions and DNA-protein mixtures...
February 14, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28192955/microfluidic-migration-and-wound-healing-assay-based-on-mechanically-induced-injuries-of-defined-and-highly-reproducible-areas
#12
Drago Sticker, Sarah Lechner, Christian Jungreuthmayer, Jürgen Zanghellini, Peter Ertl
All cell migration and wound healing assays are based on the inherent ability of adherent cells to move into adjacent cell-free areas, thus providing information on cell culture viability, cellular mechanisms and multicellular movements. Despite their widespread use for toxicological screening, biomedical research and pharmaceutical studies, to date no satisfactory technological solutions are available for the automated, miniaturized and integrated induction of defined wound areas. To bridge this technological gap, we have developed a lab-on-a-chip capable of mechanically inducing circular cell-free areas within confluent cell layers...
January 31, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28192927/improving-the-resolution-of-3d-printed-molds-for-microfluidics-by-iterative-casting-shrinkage-cycles
#13
Miao Sun, Yanbo Xie, Jihong Zhu, Jun Li, Jan C T Eijkel
Breaking through technical barriers and cost reduction are critical issues for the development of microfluidic devices, and both rely greatly on the innovation of fabrication techniques and use of new materials. The application of 3D printing definitely accelerated the prototyping of microfluidic chips by its versatility and functionality. However, the resolution of existing 3D printing techniques is still far below that of lithography, which makes it difficult to work on the scale of single cells and near impossible for single molecule work...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28191880/single-cell-gene-expression-using-multiplex-rt-qpcr-to-characterize-heterogeneity-of-rare-lymphoid-populations
#14
Thibaut Perchet, Sylvestre Chea, Milena Hasan, Ana Cumano, Rachel Golub
Gene expression heterogeneity is an interesting feature to investigate in lymphoid populations. Gene expression in these cells varies during cell activation, stress, or stimulation. Single-cell multiplex gene expression enables the simultaneous assessment of tens of genes(1)(,)(2)(,)(3). At the single-cell level, multiplex gene expression determines population heterogeneity(4)(,)(5). It allows for the distinction of population heterogeneity by determining both the probable mix of diverse precursor stages among mature cells and also the diversity of cell responses to stimuli...
January 19, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28191587/cytotoxic-responses-of-carnosic-acid-and-doxorubicin-on-breast-cancer-cells-in-butterfly-shaped-microchips-in-comparison-to-2d-and-3d-culture
#15
Ece Yildiz-Ozturk, Sultan Gulce-Iz, Muge Anil, Ozlem Yesil-Celiktas
Two dimensional (2D) cell culture systems lack the ability to mimic in vivo conditions resulting in limitations for preclinical cell-based drug and toxicity screening assays and modelling tumor biology. Alternatively, 3D cell culture systems mimic the specificity of native tissue with better physiological integrity. In this regard, microfluidic chips have gained wide applicability for in vitro 3D cancer cell studies. The aim of this research was to develop a 3D biomimetic model comprising culture of breast cancer cells in butterfly-shaped microchip to determine the cytotoxicity of carnosic acid and doxorubicin on both estrogen dependent (MCF-7) and independent (MDA-MB231) breast cancer cells along with healthy mammary epithelial cells (MCF-10A) in 2D, 3D Matrigel™ and butterfly-shaped microchip environment...
February 13, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28191267/single-cell-digital-polymerase-chain-reaction-on-self-priming-compartmentalization-chip
#16
Qiangyuan Zhu, Lin Qiu, Yanan Xu, Guang Li, Ying Mu
Single cell analysis provides a new framework for understanding biology and disease, however, an absolute quantification of single cell gene expression still faces many challenges. Microfluidic digital polymerase chain reaction (PCR) provides a unique method to absolutely quantify the single cell gene expression, but only limited devices are developed to analyze a single cell with detection variation. This paper describes a self-priming compartmentalization (SPC) microfluidic digital polymerase chain reaction chip being capable of performing single molecule amplification from single cell...
January 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28190836/specificity-of-microrna-detection-on-a-power-free-microfluidic-chip-with-laminar-flow-assisted-dendritic-amplification
#17
Kazuki Hasegawa, Rina Negishi, Mutsuyoshi Matsumoto, Masafumi Yohda, Kazuo Hosokawa, Mizuo Maeda
MicroRNAs (miRNAs) are attracting considerable attention as potential biomarkers for the early diagnosis of cancer. We have been developing a detection method for miRNAs on a microfluidic chip with external-power-free fluid pumping and enzyme-free amplification. The assay is completed within 20 min. Here, we describe the specificity of this miRNA detection method. First, the specificity against mismatched sequences was investigated. The nonspecific detection of a 2-nucleotide mismatched sequence was negligible, while that of a 1-nucleotide mismatched sequence was observed to a reasonable extent...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28189069/multitarget-sensing-of-glucose-and-cholesterol-based-on-janus-hydrogel-microparticles
#18
Xiao-Ting Sun, Ying Zhang, Dong-Hua Zheng, Shuai Yue, Chun-Guang Yang, Zhang-Run Xu
A visualized sensing method for glucose and cholesterol was developed based on the hemispheres of the same Janus hydrogel microparticles. Single-phase and Janus hydrogel microparticles were both generated using a centrifugal microfluidic chip. For glucose sensing, concanavalin A and fluorescein labeled dextran used for competitive binding assay were encapsulated in alginate microparticles, and the fluorescence of the microparticles was positively correlated with glucose concentration. For cholesterol sensing, the microparticles embedded with γ-Fe2O3 nanoparticles were used as catalyst for the oxidation of 3,3',5,5'-Tetramethylbenzidine by H2O2, an enzymatic hydrolysis product of cholesterol...
February 7, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28186118/dynamic-in-situ-sensing-of-fluid-dispersed-2d-materials-integrated-on-microfluidic-si-chip
#19
Benjamin T Hogan, Sergey A Dyakov, Lorcan J Brennan, Salma Younesy, Tatiana S Perova, Yurii K Gun'ko, Monica F Craciun, Anna Baldycheva
In this work, we propose a novel approach for wafer-scale integration of 2D materials on CMOS photonic chip utilising methods of synthetic chemistry and microfluidics technology. We have successfully demonstrated that this approach can be used for integration of any fluid-dispersed 2D nano-objects on silicon-on-insulator photonics platform. We demonstrate for the first time that the design of an optofluidic waveguide system can be optimised to enable simultaneous in-situ Raman spectroscopy monitoring of 2D dispersed flakes during the device operation...
February 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28184395/microfluidic-platform-for-rapid-measurement-of-transepithelial-water-transport
#20
Byung-Ju Jin, A S Verkman
Water transport across epithelial monolayers is of central importance in mammalian fluid homeostasis, and epithelial aquaporin (AQP) water channels are potential drug targets. Current methods to measure transepithelial water permeability based on indicator dilution have limited accuracy and can require hours for a single measurement. We report here a microfluidics platform for rapid and accurate measurement of water transport across a conventionally cultured epithelial monolayer on a porous filter requiring only a single image obtained using a standard laboratory fluorescence microscope...
February 10, 2017: Lab on a Chip
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