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Sfp1 yeast cell cycle

Aprotim Mazumder, Laia Quiros Pesudo, Siobhan McRee, Mark Bathe, Leona D Samson
An effective response to DNA damaging agents involves modulating numerous facets of cellular homeostasis in addition to DNA repair and cell-cycle checkpoint pathways. Fluorescence microscopy-based imaging offers the opportunity to simultaneously interrogate changes in both protein level and subcellular localization in response to DNA damaging agents at the single-cell level. We report here results from screening the yeast Green Fluorescent Protein (GFP)-fusion library to investigate global cellular protein reorganization on exposure to the alkylating agent methyl methanesulfonate (MMS)...
November 2013: Nucleic Acids Research
Chasity B Coleman, Patricia L Allen, James M Valles, Timothy G Hammond
To understand the cellular effects of magnetic traps requires independent analysis of the effects of magnetic field, gravity, and buoyancy. In the current study, buoyancy is manipulated by addition of Ficoll, a viscous substance that can create gradients of buoyancy without significantly affecting osmolality. Specifically, we investigated whether Ficoll induces concentration dependent changes in cell growth, cell cycle, and gene expression in Saccharomyces cerevisiae, with special attention paid to the neutrally buoyant concentration of 35% Ficoll...
June 1, 2008: Biotechnology and Bioengineering
Chasity B Coleman, Romer A Gonzalez-Villalobos, Patricia L Allen, Kelly Johanson, Karine Guevorkian, James M Valles, Timothy G Hammond
Inhomogeneous magnetic fields are used in magnetic traps to levitate biological specimens by exploiting the natural diamagnetism of virtually all materials. Using Saccharomyces cerevisiae, this report investigates whether magnetic field (B) induces changes in growth, cell cycle, and gene expression. Comparison to the effects of gravity and temperature allowed determination of whether the responses are general pathways or stimulus specific. Growth and cell cycle analysis were examined in wild-type (WT) yeast and strains with deletions in transcription factors Msn4 or Sfp1...
November 1, 2007: Biotechnology and Bioengineering
Chiara Cipollina, Lilia Alberghina, Danilo Porro, Marina Vai
Saccharomyces cerevisiae grows fast on glucose, while growth slows down on ethanol as cells move from glucose fermentation to oxidative metabolism. The type of carbon source influences both the specific growth rate and cell cycle progression, as well as cell size. Yeast cells grown on glucose have a larger size than cells grown on ethanol. Here, we analysed the behaviour of a sfp1 null mutant during balanced and transitory states of growth in batch in response to changes in the growth medium carbon sources...
April 15, 2005: Yeast
Paul Jorgensen, Ivan Rupes, Jeffrey R Sharom, Lisa Schneper, James R Broach, Mike Tyers
Cell-size homeostasis entails a fundamental balance between growth and division. The budding yeast Saccharomyces cerevisiae establishes this balance by enforcing growth to a critical cell size prior to cell cycle commitment (Start) in late G1 phase. Nutrients modulate the critical size threshold, such that cells are large in rich medium and small in poor medium. Here, we show that two potent negative regulators of Start, Sfp1 and Sch9, are activators of the ribosomal protein (RP) and ribosome biogenesis (Ribi) regulons, the transcriptional programs that dictate ribosome synthesis rate in accord with environmental and intracellular conditions...
October 15, 2004: Genes & Development
Paul Jorgensen, Joy L Nishikawa, Bobby-Joe Breitkreutz, Mike Tyers
Size homeostasis in budding yeast requires that cells grow to a critical size before commitment to division in the late prereplicative growth phase of the cell cycle, an event termed Start. We determined cell size distributions for the complete set of approximately 6000 Saccharomyces cerevisiae gene deletion strains and identified approximately 500 abnormally small (whi) or large (lge) mutants. Genetic analysis revealed a complex network of newly found factors that govern critical cell size at Start, the most potent of which were Sfp1, Sch9, Cdh1, Prs3, and Whi5...
July 19, 2002: Science
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