Jogeswar Satchidananda Purohit, Madhulika Singh, Yashankita Raghuvanshi, Saima Syeda, Madan M Chaturvedi
The N-terminus of Histone H3 is proteolytically processed in aged chicken liver. A histone H3 N-terminus specific endopeptidase (named H3ase) has been purified from the nuclear extract of aged chicken liver. By sequencing and a series of biochemical methods including the demonstration of H3ase activity in bacterially expressed GDH, it was established that the H3ase activity was a moonlighting protease activity of glutamate dehydrogenase (GDH). However, the active site for the H3ase in the GDH remains elusive...
December 1, 2023: Cell Biochemistry and Biophysics