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freezed dried plasma fibrinogen

Geir Strandenes, Ivar Austlid, Torunn O Apelseth, Tor A Hervig, Jan Sommerfelt-Pettersen, Maryanne C Herzig, Andrew P Cap, Heather F Pidcoke, Einar K Kristoffersen
BACKGROUND: Formulation of a medical preparedness plan for treating severely bleeding casualties during naval deployment is a significant challenge because of territory covered during most missions. The aim of this study was to evaluate the concept of "walking blood bank" as a supportable plan for supplying safe blood and blood products. METHODS: In 2013, the Royal Norwegian Navy conducted antipiracy operations from a frigate, beginning in the Gulf of Aden and ending in the Indian Ocean...
June 2015: Journal of Trauma and Acute Care Surgery
John R Hess, Charles C M Lelkens, John B Holcomb, Thomas M Scalea
Two decades of war in south-west Asia has demonstrated the essential role of primary resuscitation with blood products in the care of critically injured soldiers. This idea has been widely adopted and is being critically tested in civilian trauma centers. The need for red cells, plasma and platelets to be immediately available in remote locations creates a logistic burden that will best be eased by innovative new blood products such as longer-stored liquid RBCs, freeze-dried plasma, small-volume frozen platelets, and coagulation factor concentrates such as fibrinogen concentrates and prothrombin complex concentrates...
December 2013: Transfusion and Apheresis Science
Kenichi A Tanaka, Katherine Egan, Fania Szlam, Satoru Ogawa, John D Roback, Gautam Sreeram, Robert A Guyton, Edward P Chen
BACKGROUND: Platelet (PLT) and plasma transfusion remain the mainstay hemostatic therapy for perioperative bleeding. Several studies have indicated that acquired fibrinogen (FIB) deficiency can be the primary cause of bleeding after cardiac surgery. The aim of this study was to compare hematologic and transfusion profiles between the first-line FIB replacement and PLT transfusion in post-cardiac surgical bleeding. STUDY DESIGN AND METHODS: In this prospective, randomized, open-label study, 20 adult patients who underwent valve replacement or repair and fulfilled preset visual bleeding scale were randomized to 4 g of FIB or 1 unit of apheresis PLTs...
January 2014: Transfusion
Jürgen Bux, Dieter Dickhörner, Edgar Scheel
BACKGROUND: Transfusion of plasma is a basic treatment for complex coagulopathies as well as in major blood loss. Early transfusion of plasma after trauma with major hemorrhage has been recommended by retrospective studies. However, the use of plasma is often hampered by the need to maintain a cold chain and the time needed for thawing fresh-frozen plasma (FFP). With freeze-dried (lyophilized) plasma (FDP) both difficulties can be avoided. Here, we describe the production, quality characteristics, and our experiences with FDP...
December 2013: Transfusion
Christophe Martinaud, Sylvain Ausset, Anne Virginie Deshayes, Amandine Cauet, Nicolas Demazeau, Anne Sailliol
BACKGROUND: Modern warfare causes severe injuries, and despite rapid transportation to theater regional trauma centers, casualties frequently arrive coagulopathic and in shock. Massive hemorrhage management includes transfusion of red blood cells and plasma in a 1:1 ratio. Fresh frozen plasma requires thawing and badly fits the emergency criteria. Since 1994, the French Military Blood Bank has been producing freeze-dried plasma (FDP) and providing it for overseas operation. The aim of our study was to evaluate the use of FDP in war settings and to assess its clinical efficiency and safety...
December 2011: Journal of Trauma
Fahad Shuja, Robert A Finkelstein, Eugene Fukudome, Michael Duggan, Tareq Kheirbek, Kristopher Hamwi, Thomas H Fischer, Karim Fikry, Marc deMoya, George C Velmahos, Hasan B Alam
BACKGROUND: Trauma-associated coagulopathy carries an extremely high mortality. Fresh-frozen plasma (FFP) is the mainstay of treatment; however, its availability in the battlefield is limited. We have already shown that lyophilized, freeze-dried plasma (FDP) reconstituted in its original volume can reverse trauma-associated coagulopathy. To enhance the logistical advantage (lower volume and weight), we developed and tested a hyperoncotic, hyperosmotic spray-dried plasma (SDP) product in a multiple injuries/hemorrhagic shock swine model...
March 2011: Journal of Trauma
Helge Schoenfeld, Axel Pruss, Mareike Keller, Michael Schuster, Kristian Meinck, Bruno Neuner, Christian von Heymann
AIMS: Little is known about long-term stability of clotting factors in dissolved human lyophilised plasma. This study evaluated clotting factor and inhibitor activity in reconstituted lyophilised plasma after storage for up to 6 days at 4 degrees C. METHODS: Five samples from different lots of pooled lyophilised plasma (LyoPlas; German Red Cross Blood Transfusion Service West) were reconstituted. The activity of fibrinogen, factor II (FII), FV, FVII, FVIII, FIX, FX, FXI, FXII, FXIII, antithrombin, plasmin inhibitor, von Willebrand factor antigen, free protein S and protein C were determined immediately and at 2, 4, 6, 24, 48, 72, 96, 120 and 144 h after reconstitution...
August 2010: Journal of Clinical Pathology
Benny Sørensen, David Bevan
Maintaining the plasma fibrinogen concentration is important to limit excessive perioperative blood loss. This article considers the evidence for this statement, and questions the justification for using cryoprecipitate rather than virus-inactivated fibrinogen concentrate to support plasma fibrinogen levels. Haemophilia was historically treated with cryoprecipitate, but specific coagulation factor concentrates are now preferred. In contrast, primary fractions of allogeneic donor blood, including cryoprecipitate, are still commonly used to treat perioperative bleeding...
June 2010: British Journal of Haematology
Fahad Shuja, Christian Shults, Michael Duggan, Malek Tabbara, Muhammad U Butt, Thomas H Fischer, Martin A Schreiber, Brandon Tieu, John B Holcomb, Jill L Sondeen, Marc Demoya, George C Velmahos, Hasan B Alam
BACKGROUND: Trauma-induced coagulopathy is associated with an extremely high mortality. We have recently shown that survival can be improved by correction of coagulopathy through early, aggressive infusion of Fresh Frozen Plasma (FFP). However, FFP is a perishable product, and its use is impractical in challenging environments such as a battlefield. Development of shelf-stable, easy to use, low volume, lyophilized, Freeze-Dried Plasma (FDP) can overcome the logistical limitations. We hereby report the development and testing of such a product...
November 2008: Journal of Trauma
M Yunoki, S Yamamoto, H Tanaka, H Nishigaki, Y Tanaka, A Nishida, J Adan-Kubo, M Tsujikawa, S Hattori, T Urayama, M Yoshikawa, I Yamamoto, K Hagiwara, K Ikuta
BACKGROUND AND OBJECTIVE: To investigate the physico-chemical properties of hepatitis E virus (HEV) with regard to inactivation/removal, we have studied four isolates with respect to sensitivity to heat during liquid/dry-heating as well as removal by nanofiltration. MATERIALS AND METHODS: Hepatitis E virus in an albumin solution or phosphate-buffered saline (PBS) was liquid-heated at 60 degrees C for a preset time. HEV in a freeze-dried fibrinogen containing stabilizers was also dry-heated at 60 or 80 degrees C for a preset time...
August 2008: Vox Sanguinis
Peter Hellstern
Three types of therapeutic plasma are available that differ in their manufacturing processes, composition, clinical efficacy, and side effects. Quarantine-stored, not pathogen-reduced fresh-frozen plasma (QFFP) is prepared from single whole blood or plasma donations. The manufacture of pathogen-reduced single-donor plasmas such as methylene blue-light treated (MLP) or amotosalen-ultraviolet light treated plasma (ALP) involves the addition of a chemical followed by irradiation and subsequent removal of the chemical...
August 2008: Transfusion and Apheresis Science
Haiguang Zhao, Lie Ma, Jie Zhou, Zhengwei Mao, Changyou Gao, Jiacong Shen
The fast development of tissue engineering and regenerative medicine drives the old biomaterials, for example, fibrin glue, to find new applications in these areas. Aiming at developing a commercially available hydrogel for cell entrapment and delivery, in this study we optimized the fabrication and gelation conditions of fibrin gel. Fibrinogen was isolated from human plasma by a freeze-thaw circle. Gelation of the fibrinogen was accomplished by mixing with thrombin. Absorbance of the fibrinogen/thrombin mixture at 550 nm as a function of reaction time was monitored by UV-VIS spectroscopy...
March 2008: Biomedical Materials
Maria Leontowicz, Hanna Leontowicz, Zenon Jastrzebski, Iwona Jesion, Ratiporn Haruenkit, Sumitra Poovarodom, Elena Katrich, Zev Tashma, Jerzy Drzewiecki, Simon Trakhtenberg, Shela Gorinstein
The aim of this investigation was to assess the nutritional and health properties of Mon Thong durian cultivar at different stages of ripening. The assessment was carried out in vitro and in vivo. The contents of dietary fibers, minerals and trace metals at different stages of ripening were comparable. Total polyphenols (mgGAE/100 g FW) and flavonoids (mg CE/100 gFW) in ripe durian (358.8 +/- 31.4 and 95.4 +/- 9.3) were significantly higher (p < 0.05) than in mature (216.1 +/- 1 and 39.9 +/- 3.8) and overripe (283...
2007: BioFactors
Etheresia Pretorius, Sharon Briedenhann, Jorika Marx, Eureka Smit, Chris Van Der Merwe, Marlien Pieters, Carl Franz
The aim of the current study was to investigate the ultrastructural morphology of three different sources of fibrin networks and platelets, namely, lyophilized human platelet-rich plasma (LPRP), freshly prepared human platelet-rich plasma (FPRP), and human platelet concentrate (HPC). The ultrastructural morphology of the three different fibrin networks was studied using the scanning electron microscope (SEM). Turbidity curves were drawn at 405 nm at room temperature and fibrinogen concentrations were measured...
February 2007: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
Anny Appert-Flory, Florence Fischer, Didier Jambou, Pierre Toulon
INTRODUCTION: The ACL TOP is a fully-automated random-access multiparameter coagulation analyzer equipped with a photo-optical clot-detection unit. It is designed to perform coagulation, chromogenic and immunologic assays with continuous loading capabilities for samples, reagents and disposables. MATERIALS AND METHODS: The instrument was evaluated in a coagulation laboratory of a university hospital with respect to its technical features in the determination of routine coagulation (prothrombin time, activated partial thromboplastin time, fibrinogen and single coagulation factor levels), chromogenic (anti-activated factor X, antithrombin and protein C activities) and immunologic assays (free protein S and von Willebrand factor antigen concentrations)...
2007: Thrombosis Research
No abstract text is available yet for this article.
April 25, 1964: Nederlands Tijdschrift Voor Geneeskunde
Marlien Pieters, Johann C Jerling, John W Weisel
INTRODUCTION: We investigated the effect of freezing, freeze-drying and the duration of frozen storage of blood plasma on fibrin network characteristics of clots subsequently produced. MATERIALS AND METHODS: Fibrin network characteristics of clots made from freeze-dried and frozen plasma were compared to those made from fresh plasma. Freeze-dried pooled plasma was reconstituted and frozen each month for 4 months to describe the differences in fibrin networks that occur as a result of storage of the plasma over this period...
September 1, 2002: Thrombosis Research
Torstein Jensen, Sigrun Halvorsen, Hans C Godal, Ole H Skjønsberg
Freeze-dried plasma standards are often used to calibrate fibrinogen assays. Little is known, however, about the effect of freeze-drying on the clotting properties of fibrinogen. If these properties are altered, the use of freeze-dried calibration standards might influence the results obtained when applying clotting assays to determine fibrinogen concentrations. In order to disclose any discrepancies in fibrinogen concentrations before and after freeze-drying, we determined the fibrinogen concentrations in citrated plasma samples using a total clottable protein method and a clotting-rate assay before and after freeze-drying...
March 15, 2002: Thrombosis Research
C M Whitton, D Sands, A R Hubbard, P J Gaffney
An International Collaborative Study involving 12 laboratories in 7 different countries was undertaken in order to replace the 1st International Standard (IS) for Fibrinogen, Plasma (89/644). The candidate replacement standard was the ampouled and freeze-dried residue of solvent/detergent treated plasma and was calibrated as coded duplicates (A and B) versus the 1st IS Fibrinogen, Plasma by automated Clauss assay and by a recommended clot collection (gravimetric) assay. This latter method had been used to calibrate the 1st IS Fibrinogen, Plasma...
August 2000: Thrombosis and Haemostasis
Y S Kim, M K Pyo, K M Park, B S Hahn, K Y Yang, H S Yun-Choi
The freeze-dried powder of Lumbricus rubellus earthworm was administered orally to rats and its fibrinolytic and antithrombotic effects were investigated. The fibrinolytic activity of plasma was determined by measuring the plasmin activity of the euglobulin fraction and was increased to two-folds of the control at a dose of 0.5 g/kg/day and five times with 1 g/kg/day after 4-day administration. The antithrombotic effect was studied in an arterio-venous shunt model of rats. The thrombus weight decreased significantly from 43...
August 1998: Archives of Pharmacal Research
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