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lyophilized plasma fibrinogen

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https://www.readbyqxmd.com/read/25705417/role-of-prothrombin-complex-concentrate-in-perioperative-coagulation-therapy
#1
REVIEW
Kenichi A Tanaka, Michael Mazzeffi, Miroslav Durila
Prothrombin complex concentrate (PCC) is a term to describe pharmacological products that contain lyophilized, human plasma-derived vitamin K-dependent factors (F), FII, FVII, FIX, FX, and various amounts of proteins C and S. PCCs can be rapidly reconstituted in a small volume (20 ml for about 500 international units (IU)) at bedside and administered regardless of the patient's blood type. PCCs are categorized as 4-factor PCC if they contain therapeutic amounts of FVII, and 3-factor PCC when FVII content is low...
2014: Journal of Intensive Care
https://www.readbyqxmd.com/read/25460592/aqueous-extract-from-brownea-grandiceps-flowers-with-effect-on-coagulation-and-fibrinolytic-system
#2
Betzabeth Pereira, Josmary Brazón
ETHNOPHARMACOLOGICAL RELEVANCE: Brownea grandiceps flowers are used in Venezuelan folk medicine as anti-hemorrhagic in women with heavy menstrual blood loss (menorrhagia). However, prior to this study, there were no scientific investigations to support this fact, because the aqueous extract from Brownea grandiceps flowers had not been previously evaluated neither phytochemically nor biologically. The objective of this work was to evaluate in vitro the effects of aqueous extract from Brownea grandiceps flowers on the coagulation system and fibrinolysis...
February 3, 2015: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/24445628/the-effectiveness-of-different-functional-fibrinogen-polymerization-assays-in-eliminating-platelet-contribution-to-clot-strength-in-thromboelastometry
#3
Christoph J Schlimp, Cristina Solomon, Marco Ranucci, Gerald Hochleitner, Heinz Redl, Herbert Schöchl
BACKGROUND: Viscoelastic tests such as functional fibrinogen polymerization assays (FFPAs) in thrombelastography (TEG®) or thromboelastometry (ROTEM®) measure clot elasticity under platelet inhibition. Incomplete platelet inhibition influences maximum clot firmness (MCF) of FFPAs. We compared the ability of existing and newly developed FFPAs to eliminate the platelet contribution to clot strength. METHODS: MCF of whole blood (WB), platelet-rich plasma (PRP), and platelet-poor plasma samples was recorded using a ROTEM device with different FFPAs, including the TEG functional fibrinogen test (FFTEG) and different ROTEM-based assays: the standard fib-tem reagent (FIBTEM), a lyophilized single-portion reagent fib-tem S (FIBTEM-S), a newly developed reagent FIBTEM PLUS, as well as FIBTEM or the standard extrinsic activation reagent ex-tem® (EXTEM) combined with 10-μg abciximab (FIBTEM-ABC/EXTEM-ABC)...
February 2014: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/23928743/hyperosmolar-reconstituted-lyophilized-plasma-is-an-effective-low-volume-hemostatic-resuscitation-fluid-for-trauma
#4
Tim H Lee, Kate Watson, Loïc Fabricant, Jeffrey Barton, Jerome Differding, Igor Kremenevskiy, Claire Sands, Connor Wiles, Jennifer M Watters, Martin A Schreiber
BACKGROUND: We performed this study to optimize reconstituted lyophilized plasma (LP) into a minimal volume fluid that provides effective hemostatic resuscitation for trauma while minimizing logistical limitations. METHODS: We performed a prospective, blinded animal study. Plasma was lyophilized following whole blood collection from anesthetized swine. The minimal volume needed for reconstitution was determined, and this solution was evaluated for safe infusion into the swine...
September 2013: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/23718572/transfusion-and-hematologic-variables-after-fibrinogen-or-platelet-transfusion-in-valve-replacement-surgery-preliminary-data-of-purified-lyophilized-human-fibrinogen-concentrate-versus-conventional-transfusion
#5
RANDOMIZED CONTROLLED TRIAL
Kenichi A Tanaka, Katherine Egan, Fania Szlam, Satoru Ogawa, John D Roback, Gautam Sreeram, Robert A Guyton, Edward P Chen
BACKGROUND: Platelet (PLT) and plasma transfusion remain the mainstay hemostatic therapy for perioperative bleeding. Several studies have indicated that acquired fibrinogen (FIB) deficiency can be the primary cause of bleeding after cardiac surgery. The aim of this study was to compare hematologic and transfusion profiles between the first-line FIB replacement and PLT transfusion in post-cardiac surgical bleeding. STUDY DESIGN AND METHODS: In this prospective, randomized, open-label study, 20 adult patients who underwent valve replacement or repair and fulfilled preset visual bleeding scale were randomized to 4 g of FIB or 1 unit of apheresis PLTs...
January 2014: Transfusion
https://www.readbyqxmd.com/read/23581390/quality-of-freeze-dried-lyophilized-quarantined-single-donor-plasma
#6
Jürgen Bux, Dieter Dickhörner, Edgar Scheel
BACKGROUND: Transfusion of plasma is a basic treatment for complex coagulopathies as well as in major blood loss. Early transfusion of plasma after trauma with major hemorrhage has been recommended by retrospective studies. However, the use of plasma is often hampered by the need to maintain a cold chain and the time needed for thawing fresh-frozen plasma (FFP). With freeze-dried (lyophilized) plasma (FDP) both difficulties can be avoided. Here, we describe the production, quality characteristics, and our experiences with FDP...
December 2013: Transfusion
https://www.readbyqxmd.com/read/23462378/purification-characterization-and-gene-cloning-of-da-36-a-novel-serine-protease-from-deinagkistrodon-acutus-venom
#7
Ying Zheng, Feng-Ping Ye, Jie Wang, Guo-Yang Liao, Yun Zhang, Quan-Shui Fan, Wen-Hui Lee
A serine protease termed Da-36 was isolated from crude venom of Deinagkistrodon acutus. The enzyme was a single chain protein with an apparent molecular weight of 36,000 on SDS-PAGE with an isoelectric point of 6.59. Da-36 could clot human plasma by cleaving the Aα, Bβ and γ chains of fibrinogen and also exhibited arginine esterase activity. The proteolytic activity of Da-36 toward TAME was strongly inhibited by PMSF and moderately affected by benzamidine and aprotinin, indicating that it was a serine protease...
June 1, 2013: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/23321555/potential-value-of-pharmacological-protocols-in-trauma
#8
REVIEW
Herbert Schöchl, Christoph J Schlimp, Wolfgang Voelckel
PURPOSE OF REVIEW: Diagnosis and treatment of trauma-induced coagulopathy (TIC) presents a challenge for trauma care providers. Viscoelastic tests (VETs) including thromboelastometry and thrombelastography are increasingly used to diagnose TIC and guide hemostatic therapy. We summarize the concept of individualized, goal-directed coagulation management using coagulation factor concentrates. RECENT FINDINGS: Early and aggressive treatment is mandatory to improve the survival of severely bleeding trauma patients...
April 2013: Current Opinion in Anaesthesiology
https://www.readbyqxmd.com/read/21965371/fibrinogen-and-hemostasis-a-primary-hemostatic-target-for-the-management-of-acquired-bleeding
#9
REVIEW
Jerrold H Levy, Fania Szlam, Kenichi A Tanaka, Roman M Sniecienski
Fibrinogen plays several key roles in the maintenance of hemostasis. Its cleavage by thrombin and subsequent polymerization to form fibrin strands provides the structural network required for effective clot formation. During cases of acute blood loss, attempts to maintain circulating volume and tissue perfusion often involve the infusion of crystalloids, colloids, and red blood cells. Intravascular volume resuscitation, although vital, frequently results in dilution of the remaining clotting factors and onset of dilutional coagulopathy...
February 2012: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/21610357/development-and-testing-of-low-volume-hyperoncotic-hyperosmotic-spray-dried-plasma-for-the-treatment-of-trauma-associated-coagulopathy
#10
Fahad Shuja, Robert A Finkelstein, Eugene Fukudome, Michael Duggan, Tareq Kheirbek, Kristopher Hamwi, Thomas H Fischer, Karim Fikry, Marc deMoya, George C Velmahos, Hasan B Alam
BACKGROUND: Trauma-associated coagulopathy carries an extremely high mortality. Fresh-frozen plasma (FFP) is the mainstay of treatment; however, its availability in the battlefield is limited. We have already shown that lyophilized, freeze-dried plasma (FDP) reconstituted in its original volume can reverse trauma-associated coagulopathy. To enhance the logistical advantage (lower volume and weight), we developed and tested a hyperoncotic, hyperosmotic spray-dried plasma (SDP) product in a multiple injuries/hemorrhagic shock swine model...
March 2011: Journal of Trauma
https://www.readbyqxmd.com/read/21116496/effect-of-oxidation-modified-fibrinogen-on-the-formation-and-lysis-of-fibrin-clot-in-the-plasma
#11
A P Piryazev, A V Aseichev, O A Azizova
Turbidimetry studies showed that after addition of thrombin to fresh donor plasma light scatter in the sample increases and slowly attains a plateau. The process of fibrin formation was less intensive in the presence of oxidized fibrinogen. The formation of fibrin clot in lyophilized plasma was characterized by a biphasic kinetic of light scatter, oxidized fibrinogen inhibited both phases of the process. In the presence of streptokinase, oxidized fibrinogen did not modify the kinetics of fibrin clot lysis. Addition of oxidized fibrinogen to plasma reduced optical density of fibrin clot the more intensely the higher was the degree of oxidative modification of fibrinogen...
December 2009: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/20454754/-thromboelastography-metry-and-external-quality-control-results-of-a-pilot-study
#12
A Dick, M Schwaiger, C Jámbor
Thromboelastography/thromboelastometry (TEG/ROTEM) is widely used in near-patient setting, especially in perioperative and intensive care medicine for the management of acute bleeding. Until now a comprehensive quality management especially an external quality control of TEG/ROTEM results is not established. Here we report about our results of a pilot survey performed in 2008 and 2009 integrated in the External Quality Assessment Schemes (EQAS) performed by INSTAND. According to this first EQAS data ROTEM results can be controlled in external quality schemes using lyophilized plasma samples...
May 2010: Hämostaseologie
https://www.readbyqxmd.com/read/20109654/results-of-an-international-multicentre-pharmacokinetic-trial-in-congenital-fibrinogen-deficiency
#13
MULTICENTER STUDY
Flora Peyvandi
Rare bleeding disorders represent 3-5% of all inherited coagulation factor disorders. Inherited afibrinogenemia, which is caused by a deficiency in plasma fibrinogen (coagulation factor I), accounts for only a small portion of all the rare bleeding disorders. Patients with afibrinogenemia have a variable bleeding pattern that may include potentially serious or life-threatening haemorrhages. Treatment of afibrinogenemia is aimed at replacing the missing fibrinogen to restore efficient haemostasis and to stop bleeding...
December 2009: Thrombosis Research
https://www.readbyqxmd.com/read/19001961/development-and-testing-of-freeze-dried-plasma-for-the-treatment-of-trauma-associated-coagulopathy
#14
Fahad Shuja, Christian Shults, Michael Duggan, Malek Tabbara, Muhammad U Butt, Thomas H Fischer, Martin A Schreiber, Brandon Tieu, John B Holcomb, Jill L Sondeen, Marc Demoya, George C Velmahos, Hasan B Alam
BACKGROUND: Trauma-induced coagulopathy is associated with an extremely high mortality. We have recently shown that survival can be improved by correction of coagulopathy through early, aggressive infusion of Fresh Frozen Plasma (FFP). However, FFP is a perishable product, and its use is impractical in challenging environments such as a battlefield. Development of shelf-stable, easy to use, low volume, lyophilized, Freeze-Dried Plasma (FDP) can overcome the logistical limitations. We hereby report the development and testing of such a product...
November 2008: Journal of Trauma
https://www.readbyqxmd.com/read/18924717/quality-control-material-for-plasma-fibrinogen-test-produced-from-purified-human-fibrinogen
#15
Masahiro Okuda, Yahiro Uemura, Noriyuki Tatsumi
Plasma fibrinogen measurement is a routine laboratory procedure commonly performed on automated coagulation analysers. Its determination is quantitative, not quantitative. Yet, a lack of precision has been an issue for fibrinogen measurement. A control material derived from plasma comprises many proteins, inhibitors and fatty acids, any or all of which can interfere in the fibrinogen assay. This study has attempted to develop a quality control material using purified human fibrinogen and has compared measurement precision between both purified and plasma materials...
2003: Journal of Automated Methods & Management in Chemistry
https://www.readbyqxmd.com/read/18854274/human-derived-d-dimer-for-external-quality-assessment-results-of-four-surveys-in-ontario
#16
MULTICENTER STUDY
Mark A Crowther, Shannon M Bates, Michael Keeney, Anne Raby, Greg Flynn
External quality assessment (EQA) of D-dimer assays has been limited by a lack of standardized human-derived testing material. In 2006 and 2007, the Quality Management Program--Laboratory Services, Toronto, Canada, investigated the use of commercially prepared lyophilized human plasma spiked with human-derived D-dimer components manufactured by Affinity Biologicals, Hamilton, Canada. Four surveys were performed. Participants reported the level or presence of D-dimer using quantitative or qualitative methods...
November 2008: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/17441211/ultrastructural-comparison-of-the-morphology-of-three-different-platelet-and-fibrin-fiber-preparations
#17
COMPARATIVE STUDY
Etheresia Pretorius, Sharon Briedenhann, Jorika Marx, Eureka Smit, Chris Van Der Merwe, Marlien Pieters, Carl Franz
The aim of the current study was to investigate the ultrastructural morphology of three different sources of fibrin networks and platelets, namely, lyophilized human platelet-rich plasma (LPRP), freshly prepared human platelet-rich plasma (FPRP), and human platelet concentrate (HPC). The ultrastructural morphology of the three different fibrin networks was studied using the scanning electron microscope (SEM). Turbidity curves were drawn at 405 nm at room temperature and fibrinogen concentrations were measured...
February 2007: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/17408832/the-bioactivity-of-processed-garlic-allium-sativum-l-as-shown-in-vitro-and-in-vivo-studies-on-rats
#18
Zenon Jastrzebski, Hanna Leontowicz, Maria Leontowicz, Jacek Namiesnik, Zofia Zachwieja, Henryk Barton, Elke Pawelzik, Patricia Arancibia-Avila, Fernando Toledo, Shela Gorinstein
Garlic (Allium sativum L.) is widely used as an obligatory part in many cooked dishes loosing during this process a certain part of its bioactivity. Antioxidant capacity measured by the ferric-reducing/antioxidant power (FRAP) method and by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical assay was the highest in raw and in a short time processed garlic samples by cooking. 70 Wistar rats were randomly divided into 10 diet groups, each of seven. They were named Control, NPG, PG1, PG2, PG3, Chol, Chol/NPG, Chol/PG1, Chol/PG2 and Chol/PG3...
September 2007: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/17275069/evaluation-and-performance-characteristics-of-the-automated-coagulation-analyzer-acl-top
#19
Anny Appert-Flory, Florence Fischer, Didier Jambou, Pierre Toulon
INTRODUCTION: The ACL TOP is a fully-automated random-access multiparameter coagulation analyzer equipped with a photo-optical clot-detection unit. It is designed to perform coagulation, chromogenic and immunologic assays with continuous loading capabilities for samples, reagents and disposables. MATERIALS AND METHODS: The instrument was evaluated in a coagulation laboratory of a university hospital with respect to its technical features in the determination of routine coagulation (prothrombin time, activated partial thromboplastin time, fibrinogen and single coagulation factor levels), chromogenic (anti-activated factor X, antithrombin and protein C activities) and immunologic assays (free protein S and von Willebrand factor antigen concentrations)...
2007: Thrombosis Research
https://www.readbyqxmd.com/read/17087244/-standardization-of-coagulologic-technique-for-antithrombin-iii-estimation-and-its-clinical-application
#20
A L Berkovskiĭ, E V Sergeeva, A V Kartoshkina, A V Suvorov
A standard assay procedure for estimating antithrombin III activity was derived from the Abildgaard technique as follows: 1) to decrease errors at the preanalytical stage, by using preliminarily defibrinated lyophilized plasma-calibrator and 2) to increase measurement accuracy, by using the concentration of a fibrinogen preparation and simplifying the analytical procedure. The estimation of plasma antithrombin activity in patients with pulmonary thromboembolism or sepsis points to the changes in antithrombin III activity...
August 2006: Klinicheskaia Laboratornaia Diagnostika
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