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https://www.readbyqxmd.com/read/28441723/phenolic-compounds-isolated-from-caesalpinia-coriaria-induce-s-and-g2-m-phase-cell-cycle-arrest-differentially-and-trigger-cell-death-by-interfering-with-microtubule-dynamics-in-cancer-cell-lines
#1
Jessica Nayelli Sánchez-Carranza, Laura Alvarez, Silvia Marquina-Bahena, Enrique Salas-Vidal, Verónica Cuevas, Elizabeth W Jiménez, Rafael A Veloz G, Maelle Carraz, Leticia González-Maya
Caesalpinia coriaria (C. coriaria), also named cascalote, has been known traditionally in México for having cicatrizing and inflammatory properties. Phytochemical reports on Caesalpinia species have identified a high content of phenolic compounds and shown antineoplastic effects against cancer cells. The aim of this study was to isolate and identify the active compounds of a water:acetone:ethanol (WAE) extract of C. coriaria pods and characterize their cytotoxic effect and cell death induction in different cancer cell lines...
April 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28441631/up-regulated-baff-and-baff-receptor-expression-in-patients-with-intractable-temporal-lobe-epilepsy-and-a-pilocarpine-induced-epilepsy-rat-model
#2
Limin Ma, Ruohan Li, Hao Huang, Jinxian Yuan, Shu Ou, Tao Xu, Xinyuan Yu, Xi Liu, Yangmei Chen
PURPOSE: Some studies have suggested that BAFF and BAFFR are highly expressed in the central nervous system (CNS) and participate in inflammatory and immune associated diseases. However, whether BAFF and BAFFR are involved in the pathogenesis of epilepsy remains unknown. This study aimed to investigate the expression of BAFF and BAFFR proteins in the brains of patients with temporal lobe epilepsy (TLE) and in a pilocarpine-induced rat model of TLE to identify possible roles of the BAFF-BAFFR signaling pathway in epileptogenesis...
April 8, 2017: Seizure: the Journal of the British Epilepsy Association
https://www.readbyqxmd.com/read/28440877/bdnf-nt3-regulate-ais-location-and-affect-neuronal-excitability-in-cultured-hippocampal-neurons
#3
Yu Guo, Zi-Jun Su, Yi-Kun Chen, Zhen Chai
Plasticity of the axon initial segment (AIS) has aroused great interest in recent years because it regulates action potential initiation and neuronal excitability. AIS plasticity manifests as modulation of ion channels or variation in AIS structure. However, the mechanisms underlying structural plasticity of the AIS are not well understood. Here, we combined immunofluorescence, patch-clamp recordings, and pharmacological methods in cultured hippocampal neurons to investigate the factors participating in AIS structural plasticity during development...
April 25, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28440729/2-arachidonoylglycerol-endocannabinoid-signaling-coupled-to-metabotropic-glutamate-receptor-type-5-modulates-anxiety-like-behavior-in-the-rat-ventromedial-prefrontal-cortex
#4
Ana F Almeida-Santos, Fabricio A Moreira, Francisco S Guimaraes, Daniele C Aguiar
2-Arachidonoylglycerol and anandamide are the main endocannabinoids, which act through cannabinoid type-1 and type-2 receptors. Among its many functions, anandamide modulates anxiety-like behaviors in the ventromedial prefrontal cortex. The role of 2-arachidonoylglycerol in this region, however, has remained unclear. Here, we verified whether intra- ventromedial prefrontal cortex injection of 2-arachidonoylglycerol or URB602, a monoacylglycerol lipase inhibitor (responsible for 2-arachidonoylglycerol hydrolysis), induce anxiolytic-like effects in Wistar rats...
April 1, 2017: Journal of Psychopharmacology
https://www.readbyqxmd.com/read/28440511/simultaneously-increased-expression-of-glucocorticoid%C3%A2-induced-tumor-necrosis-factor-receptor-and-its-ligand-contributes-to-increased-interleukin%C3%A2-5-13%C3%A2-producing-group-2-innate-lymphocytes-in-murine-asthma
#5
Mengying Zhang, Jie Wan, Yunyun Xu, Danyi Zhang, Jingjing Peng, Chen Qi, Qi Guo, Sheng Xia, Zhaoliang Su, Shengjun Wang, Huaxi Xu
Glucocorticoid‑induced tumor necrosis factor receptor (GITR) is expressed at high levels on CD4+CD25+ regulatory T cells (Tregs). Following activation by its ligand (GITRL), GITR influences the activity of effector T cells and Tregs and participates in the development of numerous autoimmune and inflammatory diseases, including asthma. However, the GITR/GITRL expression level in lung tissue and its influence on group 2 innate lymphocytes (ILC2s) in asthma remains unclear. The present study detected the number of ILC2s and the expression levels of GITR and GITRL in the lung tissues of asthmatic mice by flow cytometry analysis, immunofluorescence staining and reverse transcription quantitative polymerase chain reaction...
April 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28440477/synchronized-dual-pulse-gastric-electrical-stimulation-improves-gastric-emptying-and-activates-enteric-glial-cells-via-upregulation-of-gfap-and-s100b-with-different-courses-of-subdiaphragmatic-vagotomy-in-rats
#6
Nian Wang, Shuangning Song, Jie Chen
Previous research and clinical practice have indicated that damage to the vagal nerve may seriously affect gastrointestinal physiological movement behavior. The aim of the current study was to observe the change of gastric motility, as well as enteric glial cells (EGCs) in the stomach with different courses of vagal nerve transection in rats prior to and following synchronized dual pulse gastric electrical stimulation. The gastric emptying rates were measured to assess the gastric motility. The glial markers, containing calcium binding protein (S100B) and glial fibrillary acidic protein (GFAP), were detected by reverse transcription‑quantitative polymerase chain reaction and double‑labeling immunofluorescence analysis...
April 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28440470/proliferation-and-differentiation-of-direct-co%C3%A2-culture-of-bone-marrow-mesenchymal-stem-cells-and-pigmented-cells-from-the-ciliary-margin
#7
Yan Li, Xinzheng He, Jun Li, Fangfang Ni, Qingqing Sun, Yan Zhou
Damage of retinal ganglion cells (RGCs) is the major consequence of glaucoma and regeneration of RGCs is extremely difficult once the damage has occurred. Retinal stem cells (RSCs) are considered an ideal choice for RGC regeneration. Pigmented cells from the ciliary margin (PCMs) have great retinal differentiation potential and may be an ideal RSC candidate. However, the ciliary margin is too small, so the number of cells that can be obtained is limited. Bone marrow‑derived mesenchymal stem cells (BMMSCs) are another type of stem cell that have been previously investigated for RGC regeneration...
April 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28440440/involvement-of-neurokinin-1-receptor-within-the-cerebrospinal-fluid%C3%A2-contacting-nucleus-in-visceral-pain
#8
Chao Zhang, Ying Li, Xin Wang, Yan Fei, Licai Zhang
Studies have shown that the cerebrospinal fluid‑contacting nucleus (CSF‑CN) may be associated with the transduction and regulation of pain signals. However, the role of the CSF‑CN remains to be elucidated. Emerging evidence has suggested that neurokinin 1 receptor (NK1R) is important in the development of visceral pain and hyperalgesia, however, whether NK1R exists in the CSF‑CN and its exact role in visceral pain remain to be fully elucidated. In the present study, double‑labeled immunofluorescence staining and western blot analysis were performed to investigate this...
April 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28440437/autophagy-related-protein-12-associates-with-anti-apoptotic-b-cell-lymphoma-2-to-promote-apoptosis-in-gentamicin-induced-inner-ear-hair-cell-loss
#9
Xiaofeng Ma, Yongze Liu, Waqas Muhammad, Dingding Liu, Junguo Wang, Han Zhou, Xia Gao, Xiaoyun Qian
The aim of the present study was to investigate the underlying mechanisms of autophagy in a gentamicin (GM)-induced ototoxic model, and to establish whether the blocking of autophagy significantly increases the survival of inner ear hair cells. Cochleae were carefully dissected from four day‑old C57BL/6J mice and randomly divided into three groups prior to explant culture: Control (culture medium), GM‑treated (culture medium + GM) and GM + 3-methyladenine (3-MA; culture medium + GM + 3‑MA). Transmission electron microscopy, immunofluorescence and western blotting were performed to observe the expression of the autophagy protein microtubule‑associated protein 1A/B‑light chain 3 in explant cultures treated with GM and the autophagy inhibitor 3‑MA...
April 11, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28440413/different-distributions-of-m1-and-m2-macrophages-in-a-mouse-model-of-laser-induced-choroidal-neovascularization
#10
Yedi Zhou, Shigeo Yoshida, Yuki Kubo, Takeru Yoshimura, Yoshiyuki Kobayashi, Takahito Nakama, Muneo Yamaguchi, Keijiro Ishikawa, Yuji Oshima, Tatsuro Ishibashi
Choroidal neovascularization (CNV) is a serious complication of age‑related macular degeneration. The aim of the present study was to investigate the expression and distribution of M1 and M2 macrophages in a laser‑induced CNV adult mouse model. The mRNA expression levels of M1, M2 and pan macrophage markers, and macrophage‑associated angiogenic cytokines, were determined by reverse transcription‑quantitative polymerase chain reaction. Immunofluorescence studies were performed to determine the location of the macrophages...
April 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28440399/%C3%AE-1-antitrypsin-promotes-lung-adenocarcinoma-metastasis-through-upregulating-fibronectin-expression
#11
Yan Li, Liyun Miao, Min Yu, Minke Shi, Yongsheng Wang, Jun Yang, Yonglong Xiao, Hourong Cai
α1-antitrypsin (AAT) has been recognized to be associated with lung adenocarcinoma metastasis. However, the mechanisms by which AAT promotes tumor metastasis remain to be investigated. Herein, we first examined AAT expression in a panel of formalin-fixed paraffin-embedded tumor tissues from 88 lung adenocarcinoma patients undergoing curative resection, using immunohistochemical methods. Lung adenocarcinoma patients with high AAT expression showed a significantly shorter overall survival compared to those with low AAT expression by Kaplan-Meier method (P=0...
April 18, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28440265/role-of-jun-amino-terminal-kinase-jnk-in-apoptosis-of-cavernosal-tissue-during-acute-phase-after-cavernosal-nerve-injury
#12
Won Hoon Song, Hwancheol Son, Soo Woong Kim, Jae-Seung Paick, Min Chul Cho
The present study aimed to identify which mitogen-activated protein kinase (p38 or Jun amino-terminal kinase [JNK]) was involved in cavernosal apoptosis during the acute phase after cavernosal nerve crush injury (CNCI) in rats to ameliorate apoptosis of cavernosal tissue, such as smooth muscle (SM). A total of twenty 10-week-old male Sprague-Dawley rats were divided equally into two groups: sham surgery (S) and CNCI (I). The I group approximated the clinical situation of men undergoing radical prostatectomy using two 60-second compressions of both CNs with a microsurgical vascular clamp...
April 18, 2017: Asian Journal of Andrology
https://www.readbyqxmd.com/read/28440085/actinomycin-d-synergistically-enhances-the-cytotoxicity-of-cddp-on-kb-cells-by-activating-p53-via-decreasing-p53-mdm2-complex
#13
Lin Wang, Xiao-Cong Pang, Zi-Ru Yu, Sheng-Qian Yang, Ai-Lin Liu, Jin-Hua Wang, Guan-Hua Du
The aim of this study is to investigate the synergism of low dose of actinomycin D (LDActD) to the cytotoxicity of cisplatin (CDDP) on KB cells. The role of P53 reactivation by LDActD in the synergism and its mechanism were further studied. Cell viability was determined by MTT assay. Apoptosis was determined by AnnexinV-FITC/PI staining. Mitochondrial membrane potential (MMP) was detected by JC-1 staining. Expression of proteins was detected by Western blotting (WB) and/or immunofluorescence (IF). Molecular docking of actinomycin D (ACTD) to Mouse double minute 2 homolog (MDM2) and Mouse double minute 2 homolog X (MDMX)...
April 25, 2017: Journal of Asian Natural Products Research
https://www.readbyqxmd.com/read/28439016/astrocytes-locally-translate-transcripts-in-their-peripheral-processes
#14
Kristina Sakers, Allison M Lake, Rohan Khazanchi, Rebecca Ouwenga, Michael J Vasek, Adish Dani, Joseph D Dougherty
Local translation in neuronal processes is key to the alteration of synaptic strength necessary for long-term potentiation, learning, and memory. Here, we present evidence that regulated de novo protein synthesis occurs within distal, perisynaptic astrocyte processes. Astrocyte ribosomal proteins are found adjacent to synapses in vivo, and immunofluorescent detection of peptide elongation in acute slices demonstrates robust translation in distal processes. We have also developed a biochemical approach to define candidate transcripts that are locally translated in astrocyte processes...
April 24, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28438983/light-dependent-expression-of-a-na-h-exchanger-3-like-transporter-in-the-ctenidium-of-the-giant-clam-tridacna-squamosa-can-be-related-to-increased-h-excretion-during-light-enhanced-calcification
#15
Kum C Hiong, Anh H Cao-Pham, Celine Y L Choo, Mel V Boo, Wai P Wong, Shit F Chew, Yuen K Ip
Na(+)/H(+) exchangers (NHEs) regulate intracellular pH and ionic balance by mediating H(+) efflux in exchange for Na(+) uptake in a 1:1 stoichiometry. This study aimed to obtain from the ctenidium of the giant clam Tridacna squamosa (TS) the complete cDNA sequence of a NHE3-like transporter (TSNHE3), and to determine the effect of light exposure on its mRNA expression level and protein abundance therein. The coding sequence of TSNHE3 comprised 2886 bp, encoding 961 amino acids with an estimated molecular mass of 105...
April 2017: Physiological Reports
https://www.readbyqxmd.com/read/28438716/yap1-negatively-regulates-chondrocyte-differentiation-partly-by-activating-the-%C3%AE-catenin-signaling-pathway
#16
Beining Yang, Hualing Sun, Fangfang Song, Miao Yu, Yanru Wu, Jiawei Wang
YAP1 (Yes-associated protein 1) transcriptional coactivator is a downstream gene of the Hippo signaling pathway, which controls cell proliferation and differentiation. YAP1 plays a significant role in the regulation of cartilage and bone development. However, the molecular mechanism by which YAP1 regulates chondrocyte differentiation remains to be elucidated. Immunofluorescent staining was used to visualize the localization of YAP1 expression in the mouse chondroprogenitor ATDC5 cell line. ATDC5 cells with lentivirus-vector-mediated YAP1 overexpression and knockdown were established...
April 21, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28438694/predictive-role-of-gstp1-containing-exosomes-in-chemotherapy-resistant-breast-cancer
#17
Su-Jin Yang, Dan-Dan Wang, Jian Li, Han-Zi Xu, Hong-Yu Shen, Xiu Chen, Si-Ying Zhou, Shan-Liang Zhong, Jian-Hua Zhao, Jin-Hai Tang
Anthracycline/taxane-based chemotherapy regimens are usually used as neoadjuvant chemotherapies to decrease tumour size and prevent metastasis of advanced breast cancer. However, patients have a high risk of developing chemo-resistance during treatment through still unknown mechanisms. Glutathione S-transferase P1 (GSTP1), which belongs to the family of phase II metabolic enzymes, has been reported to function in detoxifying several anti-cancer drugs by conjugating them with glutathione. Previous studies have identified GSTP1 as a predictor of prognosis and chemo-resistance in breast cancer patients, but the mechanisms governing GSTP1-dependent drug resistance are still unclear...
April 21, 2017: Gene
https://www.readbyqxmd.com/read/28438649/pathophysiological-roles-of-canstatin-on-myofibroblasts-after-myocardial-infarction-in-rats
#18
Akira Sugiyama, Muneyoshi Okada, Hideyuki Yamawaki
Myofibroblasts play an important role during remodeling process after myocardial infarction through proliferation, migration, production and degradation of extracellular matrix and contraction. Canstatin, a 24kDa polypeptide, is cleaved from α2 chain of type IV collagen, which is a major component of basement membrane around cardiomyocytes. We examined the effects of canstatin on myofibroblasts isolated from the areas of myocardial infarction. Myocardial infarction model was made by ligating left anterior descending artery of Wistar rats...
April 21, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28438630/identification-of-anti-viral-activity-of-the-cardenolides-na-k-atpase-inhibitors-against-porcine-transmissible-gastroenteritis-virus
#19
Cheng-Wei Yang, Hsin-Yu Chang, Hsing-Yu Hsu, Yue-Zhi Lee, Hsun-Shuo Chang, Ih-Sheng Chen, Shiow-Ju Lee
A series of naturally occurring cardenolides that exhibit potent anti-transmissible gastroenteritis virus (TGEV) activity in swine testicular (ST) cells has been identified. In an immunofluorescence assay, these cardenolides were found to diminish the expressions of TGEV nucleocapsid and spike protein, which was used as an indication for viral replication; block TGEV infection induced apoptosis and cytopathic effects; and impart the same trend of inhibitory activity against Na(+)/K(+)-ATPase as for anti-TGEV activity...
April 21, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28438571/effect-of-intestinal-epithelial-autophagy-on-bacterial-translocation-in-severe-acute-pancreatitis
#20
Weiwei Wen, Hongmei Zheng, Yingjian Jiang, Luqiao Huang, Dehui Li, Jian Zhang, Dianliang Zhang
BACKGROUND AND OBJECTIVE: We examined the impact of autophagy activation on bacterial translocation (BT) and tight junction (TJ) proteins in the intestinal mucosa of patients with severe acute pancreatitis (SAP). METHODS: Thirty-one SAP patients were divided into two groups, BT(+) and BT(-), according to the presence of BT in the blood, as detected by 16S rDNA sequencing. Eight healthy individuals were included in the control group. Serum endotoxin levels were measured by ELISA...
April 21, 2017: Clinics and Research in Hepatology and Gastroenterology
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