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Immunofluorescence

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https://www.readbyqxmd.com/read/29331090/enhanced-chondrogenesis-of-mesenchymal-stem-cells-over-silk-fibroin-chitosan-chondroitin-sulfate-three-dimensional-scaffold-in-dynamic-culture-condition
#1
Parinita Agrawal, Krishna Pramanik, Varshini Vishwanath, Amit Biswas, Akalabya Bissoyi, Pradeep Kumar Patra
Chondroitin sulfate (Ch) is one of the main structural components of cartilage tissue, therefore, its presence in tissue engineered scaffold is expected to enhance cartilage regeneration. Previously, silk fibroin/chitosan (SF/CS) blend was proven to be a potential biomaterial for tissue development. In this study, the effect of Ch on physicochemical and biological properties of SF/CS blend was investigated and scaffolds with 0.8 wt% Ch was found to be favorable. The scaffolds possess pore size of 37-212 µm, contact angle 46...
January 13, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29330965/intra-hepatic-bile-duct-primary-cilia-in-biliary-atresia
#2
Roberta Frassetto, Filippo Parolini, Salvatore Marceddu, Giulia Satta, Valeria Papacciuoli, Maria Antonia Pinna, Alessandra Mela, Giannina Secchi, Grazia Galleri, Roberto Manetti, Luisa Bercich, Vincenzo Villanacci, Antonio Dessanti, Roberto Antonucci, Francesco Tanda, Daniele Alberti, Kathleen B Schwarz, Maria Grazia Clemente
AIM: The etiopathogenesis of non-syndromic biliary atresia (BA) is obscure. The primary aim was to investigate intra-hepatic bile duct cilia (IHBC) in BA at the diagnosis and correlation with clinical outcome. The secondary aim was to analyze IHBC in routine paraffin-embedded liver biopsies using conventional scanning electron microscopy (SEM). METHODS: Surgical liver biopsies taken at the diagnosis from 22 BA infants (age range: 39 - 116 days) and from 8 children with non-BA chronic cholestasis (age range: 162 days -16,8 years) were evaluated for IHBC, both by immunofluorescence (IF) and by SEM...
January 13, 2018: Hepatology Research: the Official Journal of the Japan Society of Hepatology
https://www.readbyqxmd.com/read/29330937/first-time-detection-of-porcine-reproductive-and-respiratory-syndrome-virus-prrsv-infection-in-uruguay
#3
N Ramos, S Mirazo, G Castro, K Cabrera, F Osorio, J Arbiza
Within the last two decades, several high-impact viruses have emerged in the global swine population, including porcine reproductive and respiratory syndrome virus (PRRSV). In Uruguay, the more recent serological survey for PRRSV and other notifiable diseases such as Aujeszky's disease virus (ADV) and classical swine fever virus (CSFV) dated from year 2000. The main purpose of this study was to update our information on the infection status of PRRSV, ADV and CSFV in Uruguayan pig herds, in order to keep informed about the epidemiological situation of these notifiable infections in the country...
January 12, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29330488/motor-skill-learning-and-reward-consumption-differentially-affect-vta-activation
#4
Susan Leemburg, Tara Canonica, Andreas Luft
Dopamine release from the ventral tegmental area (VTA) terminals in the primary motor cortex (M1) enables motor skill acquisition. Here, we test the hypothesis that dopaminergic VTA neurons projecting to M1 are activated when rewards are obtained during motor skill acquisition, but not during task execution at plateau performance, or by rewards obtained without performing skilled movements. Rats were trained to perform a skilled reaching task for 3 days (acquisition) or 7 days (plateau). In combination with retrograde labelling of VTA-to-M1 projection neurons, double immunofluorescence for c-fos and tyrosine hydroxylase (TH) was used to assess activation of dopaminergic and non-dopaminergic VTA neurons...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29330471/slc26a3-dra-prevents-tnf-alpha-induced-barrier-dysfunction-and-dextran-sulfate-sodium-induced-acute-colitis
#5
Xiangming Ding, Dongxiao Li, Mengke Li, Han Wang, Qin He, Yunwu Wang, Hongbing Yu, Dean Tian, Qin Yu
SLC26A3 encodes a Cl-/HCO3- ion transporter that is also known as downregulated in adenoma (DRA) and is involved in HCO3-/mucus formation. The role of DRA in the epithelial barrier has not been previously established. In this study, we investigated the in vivo and in vitro mechanisms of DRA in the colon epithelial barrier. Immunofluorescence (IF) and co-immunoprecipitation (co-IP) studies reveal that DRA binds directly to tight junction (TJ) proteins and affects the expression of TJ proteins in polarized Caco-2BBe cells...
January 12, 2018: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29330357/knockdown-of-phospholipase-c%C3%AE%C2%B5-plc%C3%AE%C2%B5-inhibits-cell-proliferation-via-phosphatase-and-tensin-homolog-deleted-on-chromosome-10-pten-akt-signaling-pathway-in-human-prostate-cancer
#6
Xiao Wang, Yanru Fan, Zhongbo Du, Jiaxin Fan, Yanni Hao, Jinhua Wang, Xiaohou Wu, Chunli Luo
BACKGROUND Phospholipase Cε (PLCε), a member of the plc family, has been extensively studied to reveal its role in the regulation of different cell functions, but understanding of the underlying mechanisms remains limited. In the present study, we explored the effects of PLCε on PTEN (phosphatase and tensin homolog deleted on chromosome 10) in cell proliferation in prostate cancer cells. MATERIAL AND METHODS We assessed PLCε and PTEN expression in human benign prostate tissues compared to prostate cancer tissues by immunohistochemistry...
January 13, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29330283/centriole-overduplication-is-the-predominant-mechanism-leading-to-centrosome-amplification-in-melanoma
#7
Ryan A Denu, Maria Shabbir, Minakshi Nihal, Chandra K Singh, B Jack Longley, Mark E Burkard, Nihal Ahmad
Centrosome amplification (CA) is common in cancer and can arise by centriole overduplication or by cell doubling events, including the failure of cell division and cell-cell fusion. To assess the relative contributions of these two mechanisms, the number of centrosomes with mature/mother centrioles was examined by immunofluorescence in a tissue microarray (TMA) of human melanomas and benign nevi (n=79 and 17, respectively). The centrosomal protein 170 (CEP170) was used to identify centrosomes with mature centrioles; this is expected to be present in most centrosomes with cell doubling, but on fewer centrosomes with overduplication...
January 12, 2018: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/29329880/novel-expression-of-cd11b-in-epithelial-ovarian-cancer-potential-therapeutic-target
#8
Ghassan M Saed, Nicole M Fletcher, Michael P Diamond, Robert T Morris, Nardhy Gomez-Lopez, Ira Memaj
OBJECTIVE: The objective of this study was to determine the expression, and effect of targeting CD11b with a monoclonal antibody in ovarian cancer cells. METHODS: CD11b expression was determined in epithelial ovarian cancer (EOC) cell lines and tissues by immunofluorescence and flow cytometry. Cytotoxicity of the CD11b antibody and synergism with chemothearapeutic drugs were determined by the MTT Cell Proliferation Assay in human macrophages, normal ovarian epithelial cells, and in both sensitive and chemoresistant EOC cell lines...
January 9, 2018: Gynecologic Oncology
https://www.readbyqxmd.com/read/29329610/bacopa-monnieri-extracts-prevent-hydrogen-peroxide-induced-oxidative-damage-in-a-cellular-model-of-neuroblastoma-imr32-cells
#9
Gaurav Bhatia, Vikram Dhuna, Kshitija Dhuna, Manpreet Kaur, Jatinder Singh
Neurodegenerative diseases are the consequences of imbalance between the production of oxidative stress and its nullification by cellular defense mechanisms. Hydrogen peroxide (H2O2), a precursor of deleterious reactive oxygen species, elicits oxidative stress, resulting in severe brain injuries. Bacopa monnieri is well known for its nerve relaxing and memory enhancing properties. The present study was designed to evaluate the protective effects of extracts from Bacopa monnieri against H2O2 induced oxidative stress using a cellular model, neuroblastoma IMR32 cell line...
November 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/29329595/human-umbilical-cord-mesenchymal-stem-cell-conditioned-medium-attenuates-renal-fibrosis-by-reducing-inflammation-and-epithelial-to-mesenchymal-transition-via-the-tlr4-nf-%C3%AE%C2%BAb-signaling-pathway-in-vivo-and-in-vitro
#10
Bo Liu, Fengxia Ding, Dong Hu, Yu Zhou, Chunlan Long, Lianju Shen, Yuanyuan Zhang, Deying Zhang, Guanghui Wei
BACKGROUND: Renal fibrosis is characterized by infiltration of interstitial inflammatory cells and release of inflammatory mediators, activation and proliferation of fibroblasts, and deposition of excessive extracellular matrix (ECM). The aim of this study was to evaluate the effect of human umbilical cord-derived mesenchymal stem cell (hucMSC) conditioned medium (CM) on renal tubulointerstitial inflammation and fibrosis. METHODS: Renal interstitial fibrosis was prepared in vivo using the unilateral ureteral obstruction (UUO)...
January 12, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29329577/respiratory-explants-as-a-model-to-investigate-early-events-of-contagious-bovine-pleuropneumonia-infection
#11
Giovanni Di Teodoro, Giuseppe Marruchella, Andrea Di Provvido, Gianluca Orsini, Gaetano Federico Ronchi, Anna Rita D'Angelo, Nicola D'Alterio, Flavio Sacchini, Massimo Scacchia
Contagious bovine pleuropneumonia (CBPP) is a severe disease caused by Mycoplasma mycoides subsp. mycoides (Mmm). Knowledge on CBPP pathogenesis is fragmented and hampered by the limited availability of laboratory animal and in vitro models of investigation. The purpose of the present study is to assess respiratory explants as useful tools to study the early stages of CBPP. Explants were obtained from trachea, bronchi and lungs of slaughtered cattle, tested negative for Mycoplasma spp. and for the major bacterial and viral respiratory pathogens...
January 12, 2018: Veterinary Research
https://www.readbyqxmd.com/read/29329547/microfluidic-co-culture-of-pancreatic-tumor-spheroids-with-stellate-cells-as-a-novel-3d-model-for-investigation-of%C3%A2-stroma-mediated-cell-motility-and-drug-resistance
#12
Ji-Hyun Lee, Seul-Ki Kim, Iftikhar Ali Khawar, Su-Yeong Jeong, Seok Chung, Hyo-Jeong Kuh
BACKGROUND: Pancreatic stellate cells (PSCs), a major component of the tumor microenvironment in pancreatic cancer, play roles in cancer progression as well as drug resistance. Culturing various cells in microfluidic (microchannel) devices has proven to be a useful in studying cellular interactions and drug sensitivity. Here we present a microchannel plate-based co-culture model that integrates tumor spheroids with PSCs in a three-dimensional (3D) collagen matrix to mimic the tumor microenvironment in vivo by recapitulating epithelial-mesenchymal transition and chemoresistance...
January 12, 2018: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29328793/novel-molecular-and-phenotypic-insights-into-congenital-lung-malformations
#13
Daniel T Swarr, William H Peranteau, Jennifer Pogoriler, David B Frank, N Scott Adzick, Holly L Hedrick, Mike Morley, Su Zhou, Edward E Morrisey
RATIONALE: Disruption of normal pulmonary development is a leading cause of morbidity and mortality in infants. Congenital lung malformations are a unique model to study the molecular pathogenesis of isolated structural birth defects as they are often surgically resected. OBJECTIVES: To provide insight into the molecular pathogenesis of congenital lung malformations through analysis of cell-type and gene expression changes in these lesions. METHODS: Clinical data, and lung tissue for DNA, RNA, and histology were obtained from 58 infants undergoing surgical resection of a congenital lung lesion...
January 12, 2018: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/29328491/microrna-let-7a-regulates-cell-autophagy-by-targeting-rictor-in-gastric-cancer-cell-lines-mgc-803-and-sgc-7901
#14
Hao Fan, Mingkun Jiang, Bowen Li, Yu He, Chi Huang, Dakui Luo, Hao Xu, Li Yang, Jundong Zhou
miR-let-7a is the most widely studied miRNA, whose functions have been well-established by scientists in both carcinogenesis and progression of human cancer, including gastric cancer (GC). However, to date there is a lack of information concerning the relationship between miR-let-7a and cellular autophagy. Using western blotting and immunofluorescence, we determined that upregulation of miR-let-7a led to increased cellular autophagic level, whereas miR-let-7a suppression decreased autophagy activity in GC cells...
January 5, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29328447/high-mobility-group-protein%C3%A2-b1-silencing-promotes-susceptibility-of-retinoblastoma-cells-to-chemotherapeutic-drugs-through-downregulating-nuclear-factor-%C3%AE%C2%BAb
#15
Yong Chai, Juhua Xiao, Shouhua Zhang, Yunyan Du, Zhipeng Luo, Xin Zhou, Kai Huang
The aim of the present study was to investigate the effects of high-mobility group protein B1 (HMGB1) silencing on the susceptibility of retinoblastoma (RB) cells to chemotherapeutic drugs and the underlying molecular mechanisms. Western blot analysis revealed that vincristine (VCR), etoposide (ETO) and carboplatin (CBP) significantly increased the expression of HMGB1 in Weri‑Rb-1 and Y79 cells compared with the untreated control (P<0.01). siRNA HMGB1 and siRNA negative control (NC) were transfected to Y79 cells by Lipofectamine™ 2000 and, following VCR treatment, the expression of HMGB1 and nuclear factor-κB (NF-κB) was analyzed...
January 10, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29328444/sp1-reduces-autophagic-flux-through-activating-p62-in-gastric-cancer-cells
#16
Xiao-Wu Xu, Chen-Wei Pan, Xiao-Min Yang, Lei Zhou, Zhi-Qiang Zheng, De-Chun Li
Gastric cancer is the most common type of gastrointestinal cancer, causing mortality worldwide. However, the underlying molecular mechanism in gastric cancer progression remains unclear. The autophagic flux was determined in gastric cancer cells overexpressing or inhibiting Sp1 transcription factor (SP1) using western blotting, reverse transcription‑polymerase chain reaction and immunofluorescence staining. Luciferase and ChIP assays were performed to detect the potential underlying mechanism of SP1 in gastric cancer cells...
January 9, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29328434/chemical-conversion-of-human-lung-fibroblasts-into-neuronal-cells
#17
Xiao-Yu Wan, Li-Yun Xu, Bing Li, Qiu-Hong Sun, Qiu-Liang Ji, Dong-Dong Huang, Lan Zhao, Yong-Tao Xiao
It has been previously reported that human embryonic fibroblasts and mouse embryonic fibroblasts can be converted into neuronal cells using chemical agents, along with forced expression specific transcriptional factors. However, the materials required for reprogramming in these approaches presents major technical difficulties and safety concerns. The current study investigated whether a cocktail of small molecules can convert human lung fibroblast cells into neurons. The small molecules valproic acid, CHIR99021, DMH1, Repsox, forskolin, Y‑27632 and SP600125 (VCHRFYS) were used to induce MRC‑5 cells into neuronal cells in vitro...
January 10, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29328420/ginsenoside-rg3-inhibits-keloid-fibroblast-proliferation-angiogenesis-and-collagen-synthesis-in-vitro-via-the-tgf%C3%A2-%C3%AE-smad-and-erk-signaling-pathways
#18
Mengyao Tang, Weiwei Bian, Liying Cheng, Lu Zhang, Rong Jin, Wenbo Wang, Yuguang Zhang
A wide range of therapeutic options exists for the treatment of keloids, all of which have their own strengths; however, a high risk of side‑effects and frequent recurrence remains. Therefore, the present study aimed to identify improved therapeutic approaches or drugs for the treatment of keloids. Ginsenoside Rg3 (Rg3) has been reported to exert numerous antitumor effects, thus indicating that Rg3 may be a potential therapeutic agent that targets keloids. The present study determined the effects of Rg3 on human keloid fibroblasts (KFs) in vitro, and further explored the associated molecular and cellular mechanisms...
January 4, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29328382/propofol-postconditioning-protects-h9c2-cells-from-hypoxia-reoxygenation-injury-by-inducing-autophagy-via-the-sapk-jnk-pathway
#19
Hao Li, Xuan Zhang, Jian Tan, Li Sun, Long-He Xu, Yu-Ge Jiang, Jing-Sheng Lou, Xue-Yin Shi, Wei-Dong Mi
Propofol postconditioning (P‑PostC) offers cardioprotection in mice, and the upregulation of autophagy protects cardiac cells against ischemia/reperfusion injury. The present study aimed to examine the effects of P‑PostC on the induction of autophagy and its potential roles in hypoxia/reoxygenation (H/R) injury. Rat heart‑derived H9c2 cells were exposed to H/R, comprising 6 h of hypoxia followed by 4 h of reoxygenation, as well as postconditioning with various concentrations of propofol at the onset of reperfusion...
January 11, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29328020/the-synaptic-ribbon-is-critical-for-sound-encoding-at-high-rates-and-with-temporal-precision
#20
Philippe Jean, David Lopez de la Morena, Susann Michanski, Lina María Jaime Tobón, Rituparna Chakrabarti, Maria Magdalena Picher, Jakob Neef, SangYong Jung, Mehmet Gültas, Stephan Maxeiner, Andreas Neef, Carolin Wichmann, Nicola Strenzke, Chad Grabner, Tobias Moser
We studied the role of the synaptic ribbon for sound encoding at the synapses between inner hair cells (IHCs) and spiral ganglion neurons (SGNs) in mice lacking RIBEYE (RBEKO/KO). Electron and immunofluorescence microscopy revealed a lack of synaptic ribbons and an assembly of several small active zones (AZs) at each synaptic contact. Spontaneous and sound-evoked firing rates of SGNs and their compound action potential were reduced, indicating impaired transmission at ribbonless IHC-SGN synapses. The temporal precision of sound encoding was impaired and the recovery of SGN-firing from adaptation indicated slowed synaptic vesicle (SV) replenishment...
January 12, 2018: ELife
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