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https://www.readbyqxmd.com/read/28440536/selected-bone-morphogenetic-proteins-the-possibility-of-their-use-in-the-diagnostics-and-therapy-of-severe-asthma
#1
Marcelina Koćwin, Mateusz Jonakowski, Marcelina Przemęcka, Michał Panek, Piotr Kuna
Asthma is a chronic heterogeneous illness of the lower airway with an inflammatory basis, developing from hyperresponsiveness and bronchial obstruction. One of the more unfavourable processes occurring in the airway are the long-term changes of the respiratory tract known as remodelling, resulting in complete irreversible obstruction. Bone morphogenetic protein (BMP) is a member of the Transforming Growth Factor beta (TGF-b) superfamily, which regulates processes in embryonic and post-embryonic development...
2017: Advances in Respiratory Medicine
https://www.readbyqxmd.com/read/28440452/bambi-overexpression-together-with-%C3%AE-sitosterol-ameliorates-nsclc-via-inhibiting-autophagy-and-inactivating-tgf-%C3%AE-smad2-3-pathway
#2
Xingchun Wang, Minjie Li, Mengyao Hu, Ping Wei, Wei Zhu
Non-small cell lung cancer (NSCLC) has the highest mortality rate among all solid tumors with a poor prognosis. The BMP and activin receptor membrane bound inhibitor (BAMBI) has been identified as a hallmark of NSCLC and β-sitosterol possesses antitumor potentiality. This study explores the effect of BAMBI overexpression and β-sitosterol in the context of NSCLC. The results revealed that the transfection of pcDNA‑BAMBI and β-sitosterol treatment significantly reduced the levels of autophagy markers light chain 3 (LC3) II and Beclin 1, whereas the levels of LC3 I and p62 were promoted...
May 2017: Oncology Reports
https://www.readbyqxmd.com/read/28438858/functional-roles-of-netrin-1-in-osteoblast-differentiation
#3
Tsuyoshi Sato, Shoichiro Kokabu, Yuichiro Enoki, Naoki Hayashi, Masahito Matsumoto, Mitsuhiko Nakahira, Masashi Sugasawa, Tetsuya Yoda
AIM: Recent studies have demonstrated that netrin-1 plays a vital role in bone metabolism. Previous studies have shown that osteoblasts produce netrin-1 which affects osteoclast differentiation. However, the role of netrin-1 in osteoblast differentiation is not well understood. In this study, we explored the roles of netrin-1 in osteoblasts. MATERIALS AND METHODS: Quantitative reverse-transcriptase polymerase chain reaction (qPCR), RNA interference for netrin receptors, the generation of netrin-1 plasmid, transfection of plasmids, and cell proliferation assay were performed...
May 2017: In Vivo
https://www.readbyqxmd.com/read/28438835/il-1%C3%AE-transcriptionally-activates-hepcidin-by-inducing-c-ebp%C3%AE-expression-in-hepatocytes
#4
Yohei Kanamori, Masaru Murakami, Makoto Sugiyama, Osamu Hashimoto, Tohru Matsui, Masayuki Funaba
Hepcidin is a liver-derived hormone that negatively regulates serum iron levels and is mainly regulated at the transcriptional level. Previous studies have clarified that in addition to hepatic iron levels, inflammation also efficiently increases hepatic hepcidin expression. The principle regions responsible for efficient hepcidin transcription are bone morphogenetic protein-responsive elements (BMP-REs) 1 and 2 as well as the signal transducer and activator of transcription 3-binding site (STAT-BS). Here, we show that the proinflammatory cytokine interleukin-1β (IL-1β) efficiently increases hepcidin expression in human HepG2 liver-derived cells and primary mouse hepatocytes...
April 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28438754/smad1-5-is-required-for-erythropoietin-mediated-suppression-of-hepcidin-in-mice
#5
Chia-Yu Wang, Amanda B Core, Susanna Canali, Kimberly B Zumbrennen-Bullough, Sinan Ozer, Lieve Umans, An Zwijsen, Jodie L Babitt
Anemia suppresses liver hepcidin expression to supply adequate iron for erythropoiesis. Erythroferrone mediates hepcidin suppression by anemia, but its mechanism of action remains uncertain. The bone morphogenetic protein (BMP)-SMAD signaling pathway has a central role in hepcidin transcriptional regulation. Here, we explored the contribution of individual receptor-activated SMADs in hepcidin regulation and their involvement in erythroferrone suppression of hepcidin. In Hep3B cells, SMAD5 or SMAD1, but not SMAD8, knockdown inhibited hepcidin (HAMP) mRNA expression...
April 24, 2017: Blood
https://www.readbyqxmd.com/read/28437133/the-impact-of-the-centrifuge-characteristics-and-centrifugation-protocols-on-the-cells-growth-factors-and-fibrin-architecture-of-a-leukocyte-and-platelet-rich-fibrin-l-prf-clot-and-membrane
#6
David M Dohan Ehrenfest, Nelson R Pinto, Andrea Pereda, Paula Jiménez, Marco Del Corso, Byung-Soo Kang, Mauricio Nally, Nicole Lanata, Hom-Lay Wang, Marc Quirynen
L-PRF (leukocyte- and platelet-rich fibrin) is one of the four families of platelet concentrates for surgical use and is widely used in oral and maxillofacial regenerative therapies. The first objective of this article was to evaluate the mechanical vibrations appearing during centrifugation in four models of commercially available table-top centrifuges used to produce L-PRF and the impact of the centrifuge characteristics on the cell and fibrin architecture of a L-PRF clot and membrane. The second objective of this article was to evaluate how changing some parameters of the L-PRF protocol may influence its biological signature, independently from the characteristics of the centrifuge...
April 24, 2017: Platelets
https://www.readbyqxmd.com/read/28436605/digeorge-like-syndrome-in-a-child-with-a-3p12-3-deletion-involving-mir4273-gene-born-to-a-mother-with-gestational-diabetes-mellitus
#7
Emilia Cirillo, Giuliana Giardino, Vera Gallo, Giovanni Galasso, Roberta Romano, Roberta D'Assante, Giulia Scalia, Luigi Del Vecchio, Lucio Nitsch, Rita Genesio, Claudio Pignata
Chromosome 22q11.2 deletion is the most common chromosomal alteration associated with DiGeorge syndrome (DGS), even though this is not the only underlying cause of DGS. In rare patients, mutations in a single gene, TBX1, have been described resulting in a DGS phenotype. Recently, it has been reported that at least part of the TBX1 mutant phenotype is due to excessive bone morphogenetic proteins (BMP) signaling. Evidence suggests that miRNA may modulate the expression of critical T-box transcriptional regulators during midface development and Bmp-signaling...
April 24, 2017: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/28434979/synergistic-effects-of-bmp-2-bmp-6-or-bmp-7-with-human-plasma-fibronectin-onto-hydroxyapatite-coatings-a-comparative-study
#8
Isabelle Brigaud, Rémy Agniel, Johanne Leroy Dudal, Sabrina Kellouche, Arnaud Ponche, Tahar Bouceba, Natalia Mihailescu, Mihai Sopronyi, Eric Viguier, Carmen Ristoscu, Felix Sima, Ion N Mihailescu, Ana Claudia O Carreira, Mari Cleide Sogayar, Olivier Gallet, Karine Anselme
Design of new osteoinductive biomaterials to reproduce an optimized physiological environment capable of recruiting stem cells and instructing their fate towards the osteoblastic lineage has become a priority in orthopedic surgery. This work aims at evaluating the bioactivity of BMP combined with human plasma fibronectin (FN/BMP) delivered in solution or coated onto titanium-hydroxyapatite (TiHA) surfaces. Herein, we focus on the comparison of in vitro osteogenic efficacy in mouse C2C12 pre-osteoblasts of three BMP members, namely: BMP-2, BMP-6 and BMP-7...
April 18, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28433584/electrohydrolysis-pretreatment-of-water-hyacinth-for-enhanced-hydrolysis
#9
Visva Bharati Barua, V Wilson Raju, Sophia Lippold, Ajay S Kalamdhad
This study investigates the use of electrohydrolysis pretreatment on water hyacinth to cut short the hydrolysis step and increase biogas production at the same time. Electrohydrolysis pretreatment of water hyacinth at 20V for 60min exhibited improved solubilisation (42.9%). Therefore, bio-chemical methane potential (BMP) test was carried out between water hyacinth pretreated at 20V for 60min and untreated water hyacinth. By the end of 30days, cumulative methane production of 2455±17mL CH4/g VS for electrohydrolysis pretreated substrate and 1936±27mL CH4/g VS for the untreated substrate was achieved...
April 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28433171/gellan-gum-based-physical-hydrogels-incorporating-highly-valuable-endogen-molecules-and-associating-bmp-2-as-bone-formation-platforms
#10
Rita López-Cebral, Ana Civantos, Viviana Ramos, Begoña Seijo, José Luis López-Lacomba, José Vicente Sanz-Casado, Alejandro Sanchez
Physical hydrogels have been designed for a double purpose: as growth factor delivery systems and as scaffolds to support cell colonization and formation of new bone. Specifically, the polysaccharide gellan gum and the ubiquitous endogenous molecules chondroitin, albumin and spermidine have been used as exclusive components of these hydrogels. The mild ionotropic gelation technique was used to preserve the bioactivity of the selected growth factor, rhBMP-2. In vitro tests demonstrated the effective delivery of rhBMP-2 in its bioactive form...
July 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28427810/synthesis-of-novel-5-6-dehydrokawain-analogs-as-osteogenic-inducers-and-their-action-mechanisms
#11
Momochika Kumagai, Keisuke Nishikawa, Takashi Mishima, Izumi Yoshida, Masahiro Ide, Keiko Koizumi, Munetomo Nakamura, Yoshiki Morimoto
An imbalance between bone resorption by osteoclasts and bone formation by osteoblasts can cause bone loss and bone-related disease. In a previous search for natural products that increase osteogenic activity, we found that 5,6-dehydrokawain (1) from Alpinia zerumbet promotes osteoblastogenesis. In this study, we synthesized and evaluated series of 5,6-dehydrokawain analogs. Our structure-activity relationships revealed that alkylation of para or meta position of aromatic ring of 1 promote osteogenic activity...
April 6, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28426699/anti-il-20-monoclonal-antibody-inhibited-inflammation-and-protected-against-cartilage-destruction-in-murine-models-of-osteoarthritis
#12
Yu-Hsiang Hsu, Ya-Yu Yang, Man-Hsiang Huwang, Yun-Han Weng, I-Ming Jou, Po-Tin Wu, Tain-Yu Lin, Li-Wha Wu, Ming-Shi Chang
Osteoarthritis (OA) is a degenerative joint disease characterized by progressive destruction of articular cartilage. Interleukin (IL)-20 is a proinflammatory cytokine involved in the pathogenesis of rheumatoid arthritis. We investigated the role of IL-20 in OA and evaluated whether anti-IL-20 antibody (7E) treatment attenuates disease severity in murine models of surgery-induced OA. Immunohistochemical staining was used to detect IL-20 and its receptors expression in synovial tissue and cartilage from OA patients, and in OA synovial fibroblasts (OASFs) and chondrocytes (OACCs) from rodents with surgery-induced OA...
2017: PloS One
https://www.readbyqxmd.com/read/28426320/transient-exposure-to-androgens-induces-a-remarkable-self-sustained-quiescent-state-in-dispersed-prostate-cancer-cells
#13
Anh Thu Bui, Meng-Er Huang, Maryline Havard, Fanny Laurent-Tchenio, François Dautry, Thierry Tchenio
Cellular quiescence is a reversible cell growth arrest that is often assumed to require a persistence of non-permissive external growth conditions for its maintenance. In this work, we showed that androgen could induce a quiescent state that is self-sustained in a cell-autonomous manner through a "hit and run" mechanism in androgen receptor-expressing prostate cancer cells. This phenomenon required the set-up of a sustained redox imbalance and TGFβ/BMP signaling that were dependent on culturing cells at low density...
April 20, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28424263/the-kielin-chordin-like-protein-kcp-attenuates-high-fat-diet-induced-obesity-and-metabolic-syndrome-in-mice
#14
Abdul Soofi, Katherine I Wolf, Margo P Emont, Nathan Qi, Gabriel Martinez-Santibanez, Edward Grimley, Wesam Ostwani, Gregory R Dressler
Obesity and its associated complications, such as insulin resistance and non-alcoholic fatty liver disease, are reaching epidemic proportions. In mice, the TGF-beta superfamily is implicated in the regulation of white and brown adipose tissue differentiation. The Kielin/chordin-like Protein (KCP) is a secreted regulator of the TGF-beta superfamily pathways that can inhibit both TGF-beta and activin signals while enhancing bone morphogenetic protein (BMP) signaling. However, KCP's effects on metabolism and obesity have not been studied in animal models...
April 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28423564/hypermethylation-of-the-chrdl1-promoter-induces-proliferation-and-metastasis-by-activating-akt-and-erk-in-gastric-cancer
#15
Yao-Fei Pei, Ya-Jing Zhang, Yao Lei, Ding-Wei Wu, Tong-Hui Ma, Xi-Qiang Liu
CHRDL1 (Chordin-like 1) is a secreted protein that acts as an antagonist of bone morphogenetic protein (BMP). BMP plays a role as an activator of BMP receptor II (BMPR II), which mediates extracellular to intracellular signal transmission and is involved in carcinogenesis and metastasis. Herein, we report that CHRDL1 expression was significantly down-regulated in gastric cancer tissues and associated with poor survival. Clinic-pathological parameters demonstrated a close relationship between low CHRDL1 expression and metastasis...
April 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28420447/ck2-1-a-bone-morphogenetic-protein-receptor-type-ia-mimetic-peptide-repairs-cartilage-in-mice-with-destabilized-medial-meniscus
#16
Hemanth Akkiraju, Padma Pradeepa Srinivasan, Xian Xu, Xinqiao Jia, Catherine B Kirn Safran, Anja Nohe
BACKGROUND: Osteoarthritis (OA) of the knee involves degeneration of articular cartilage of the diarthrodial joints. Current treatment options temporarily relieve the joint pain but do not restore the lost cartilage. We recently designed a novel bone morphogenetic protein receptor type I (BMPRI) mimetic peptide, CK2.1, that activates BMPRIa signaling in the absence of bone morphogenetic protein (BMP). Our previous research demonstrated that CK2.1 induced chondrogenesis in vitro and in vivo; however, it is unknown if CK2...
April 18, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28419987/extracellular-matrix-mediates-bmp-2-in-a-model-of-temporomandibular-joint-osteoarthritis
#17
Maya Shirakura, Vardit Kram, Jennifer Robinson, Sheena Sikka, Tina M Kilts, Sunil Wadhwa, Marian F Young
Temporomandibular joint (TMJ) osteoarthritis (OA) is a complex disease that affects both cartilage and subchondral bone. It is accompanied by loss of extracellular matrix (ECM) and may be controlled by bone morphogenetic protein-2 (BMP-2). We analyzed the effect of BMP-2 in both cartilage and subchondral bone in a TMJ-OA animal model that is deficient in biglycan (Bgn) and fibromodulin (Fmod) (Bgn-/-Fmod-/-). Whole mandibles were dissected from 3-week-old wild-type (WT) and Bgn-/-Fmod-/- mice and incubated with and without 250 µg/mL BMP-2 for 2 days using an explant culture system...
April 19, 2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/28419693/ag-loaded-mgsrfe-layered-double-hydroxide-chitosan-composite-scaffold-with-enhanced-osteogenic-and-antibacterial-property-for-bone-engineering-tissue
#18
Dandan Cao, Zhengliang Xu, Yixuan Chen, Qinfei Ke, Changqing Zhang, Yaping Guo
Bone tissue engineering scaffolds for the reconstruction of large bone defects should simultaneously promote osteogenic differentiation and avoid postoperative infection. Herein, we develop, for the first time, Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold. This scaffold exhibits three-dimensional interconnected macroporous structure with a pore size of 100-300 μm. The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces...
April 17, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28419312/minimally-invasive-transforaminal-lumbar-interbody-fusion-meta-analysis-of-the-fusion-rates-what-is-the-optimal-graft-material
#19
Avelino Parajón, Marjan Alimi, Rodrigo Navarro-Ramirez, Paul Christos, Jose M Torres-Campa, Yu Moriguchi, Gernot Lang, Roger Härtl
BACKGROUND: Minimally invasive transforaminal lumbar interbody fusion (MIS-TLIF) is an increasingly popular procedure with several potential advantages over traditional open TLIF. OBJECTIVE: The current study aimed to compare fusion rates of different graft materials used in MIS-TLIF, via meta-analysis of the published literature. METHODS: A Medline search was performed and a database was created including patient's type of graft, clinical outcome, fusion rate, fusion assessment modality, and duration of follow-up...
April 17, 2017: Neurosurgery
https://www.readbyqxmd.com/read/28419135/an-organotypic-slice-culture-to-study-the-formation-of-calyx-of-held-synapses-in-vitro
#20
Elin Kronander, Nicolas Michalski, Cécile Lebrand, Jean-Pierre Hornung, Ralf Schneggenburger
The calyx of Held, a large axo-somatic relay synapse containing hundreds of presynaptic active zones, is possibly the largest nerve terminal in the mammalian CNS. Studying its initial growth in-vitro might provide insights into the specification of synaptic connection size in the developing brain. However, attempts to maintain calyces of Held in organotypic cultures have not been fruitful in past studies. Here, we describe an organotypic slice culture method in which calyces of Held form in-vitro. We made coronal brainstem slices with an optimized slice angle using newborn mice in which calyces have not yet formed; the presynaptic bushy cells were genetically labeled using the Math5 promoter...
2017: PloS One
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