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https://www.readbyqxmd.com/read/28542453/extracellular-calcium-promotes-bone-formation-from-bone-marrow-mesenchymal-stem-cells-by-amplifying-the-effects-of-bmp-2-on-smad-signalling
#1
Rubén Aquino-Martínez, Natalia Artigas, Beatriz Gámez, José Luis Rosa, Francesc Ventura
Understanding the molecular events that regulate osteoblast differentiation is essential for the development of effective approaches to bone regeneration. In this study, we analysed the osteoinductive properties of extracellular calcium in bone marrow-derived mesenchymal stem cell (BM-MSC) differentiation. We cultured BM-MSCs in 3D gelatin scaffolds with Ca2+ and BMP-2 as osteoinductive agents. Early and late osteogenic gene expression and bone regeneration in a calvarial critical-size defect model demonstrate that extracellular Ca2+ enhances the effects of BMP-2 on Osteocalcin, Runx2 and Osterix expression and promotes bone regeneration in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/28537650/tendon-healing-induced-by-chemically-modified-mrnas
#2
K Groth, T Berezhanskyy, M K Aneja, J Geiger, M Schweizer, L Maucksch, T Pasewald, T Brill, B Tigani, E Weber, C Rudolph, G Hasenpusch
Tendon disorders are frequent both in human and veterinary medicine with high re-injury rates and unsatisfactory therapeutic treatments. Application of naked, chemically-modified mRNA (cmRNA), encoding for therapeutic proteins, is an innovative approach to address tendon healing. In the current study, we demonstrated that injection of naked cmRNA, diluted in a glucose-containing solution, into tendons resulted in high protein expression in healthy and experimentally-injured tendons. Injection of bone morphogenetic protein 7 (BMP-7)-encoding cmRNA resulted in a significantly higher expression of BMP-7 protein and reduced formation of collagen type III, compared to vehicle control...
May 24, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28537529/a-versatile-protocol-for-studying-calvarial-bone-defect-healing-in-a-mouse-model
#3
Rebekah Margaret Samsonraj, Amel Dudakovic, Pengfei Zan, Oksana Pichurin, Simon Cool, Andre J van Wijnen
Animal models are vital tools for the preclinical development and testing of therapies aimed at providing solutions for several musculoskeletal disorders. For bone tissue engineering strategies addressing non-union conditions, rodent models are particularly useful for studying bone healing in a controlled environment. The mouse calvarial defect model permits evaluation of drug, growth factor or cell transplantation efficacy, together with offering the benefit of utilizing genetic models to study intramembranous bone formation within defect sites...
May 24, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28537526/composite-biomaterial-as-a-carrier-for-bone-active-substances-for-metaphyseal-tibial-bone-defect-reconstruction-in-rats
#4
Peter Frederik Horstmann, Deepak Raina, Hanna Isaksson, Werner Hettwer, Lars Lidgren, Michael Mørk Petersen, Magnus Tägil
Restoring lost bone is a major challenge in orthopedic surgery. Currently available treatment-strategies all have shortcomings, like risk of infection, non-union and excessive resorption. Our primary aim was to study if a commercially available gentamicin-containing composite calcium sulphate/hydroxyapatite biomaterial (GBM) could serve as a carrier for local delivery of bone morphogenic protein-2 (BMP-2) and zoledronic acid (ZA) in a tibia defect-model in rats. Empty and allograft-filled defects were used as controls...
May 24, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28537482/programmed-platelet-derived-growth-factor-%C3%AE-%C3%AE-and-bone-morphogenetic-protein-2-delivery-from-a-hybrid-calcium-phosphate-alginate-scaffold
#5
Emily A Bayer, Jahnelle Jordan, Riccardo Gottardi, Morgan Fedorchak, Prashant N Kumta, Steven Little
Bone tissue engineering requires the upregulation of several regenerative stages, including a critical early phase of angiogenesis. Previous studies have suggested that a sequential delivery of PDGF to BMP-2 could promote angiogenic tubule formation when delivered to in vitro co-cultures of human umbilical vein endothelial cells (HUVECs) and human mesenchymal stem cells (hMSCs). However, it was previously unclear that this PDGF to BMP-2 delivery schedule will result in cell migration into the scaffolding system and affect the later expression of bone markers...
May 24, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28536843/human-interstitial-cellular-model-in-therapeutics-of-heart-valve-calcification
#6
REVIEW
Caimei He, Hai Tang, Zijian Mei, Nichujie Li, Zhi Zeng, Kwame Oteng Darko, Yulong Yin, Chien-An Andy Hu, Xiaoping Yang
Calcific aortic valve disease is a common, severe heart condition that is currently with no proven, effective drug treatment and requires a surgical valve replacement or an entire heart explanation. Thus, developing novel, targeted therapeutic approaches becomes a major goal for cardiovascular disease research. To achieve this goal, isolated heart valve interstitial cells could be an advanced model to explore molecular mechanisms and measure drug efficacy. Based on this progress, molecular mechanisms that harbor components of  inflammation and fibrosis coupled with proteins, for example, BMP-2, TLRs, RANKL, Osteoprotegerin, have been proposed...
May 23, 2017: Amino Acids
https://www.readbyqxmd.com/read/28536788/erratum-to-bmp-type-ii-receptor-as-a-therapeutic-target-in-pulmonary-arterial-hypertension
#7
Mar Orriols, Maria Catalina Gomez-Puerto, Peter Ten Dijke
No abstract text is available yet for this article.
May 23, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28535613/expression-and-purification-of-biologically-active-human-bone-morphogenetic-protein-4-in-recombinant-chinese-hamster-ovary-cells
#8
Minyub Cha, Nara Han, Jia Pi, Yongsu Jeong, Kwanghee Baek, Jaeseung Yoon
Bone morphogenetic protein-4 (BMP-4) is considered to have therapeutic potential for various diseases including cancers; however, the high expression of biologically active recombinant human BMP-4 (rhBMP-4) needed to manufacture rhBMP-4 for therapeutic purposes has yet to be established. In the current study, we established a recombinant Chinese hamster ovary (rCHO) cell line overexpressing rhBMP-4 as well as a production process using 7.5-l bioreactor (5 l working volume). The expression of the mature rhBMP-4 was significantly enhanced by recombinant furin expression...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28535370/epithelial-patterning-morphogenesis-and-evolution-drosophila-eggshell-as-a-model
#9
REVIEW
Miriam Osterfield, Celeste A Berg, Stanislav Y Shvartsman
Understanding the mechanisms driving tissue and organ formation requires knowledge across scales. How do signaling pathways specify distinct tissue types? How does the patterning system control morphogenesis? How do these processes evolve? The Drosophila egg chamber, where EGF and BMP signaling intersect to specify unique cell types that construct epithelial tubes for specialized eggshell structures, has provided a tractable system to ask these questions. Work there has elucidated connections between scales of development, including across evolutionary scales, and fostered the development of quantitative modeling tools...
May 22, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28532689/lipid-composition-of-microdomains-is-altered-in-neuronopathic-gaucher-disease-sheep-brain-and-spleen
#10
Leanne K Hein, Tina Rozaklis, Melissa K Adams, John J Hopwood, Litsa Karageorgos
Gaucher disease is a lysosomal storage disorder caused by a deficiency in glucocerebrosidase activity that leads to accumulation of glucosylceramide and glucosylsphingosine. Membrane raft microdomains are discrete, highly organized microdomains with a unique lipid composition that provide the necessary environment for specific protein-lipid and protein-protein interactions to take place. In this study we purified detergent resistant membranes (DRM; membrane rafts) from the occipital cortex and spleen from sheep affected with acute neuronopathic Gaucher disease and wild-type controls...
May 17, 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/28532084/incorporation-of-bmp-2-loaded-collagen-conjugated-bcp-granules-in-calcium-phosphate-cement-based-injectable-bone-substitutes-for-improved-bone-regeneration
#11
Gun Hee Lee, Preeti Makkar, Kallyanshis Paul, ByongTaek Lee
The objective of the present study was to incorporate surface modified porous multichannel BCP granule into CPC to enhance its in vivo biodegradation and bone tissue growth. The multichannel BCP granule (15wt%) was first coated with collagen subsequent to BMP-2 loading (ccMCG-B). It was then embedded into CPC to form CPC-ccMCG-B system. The newly developed CPC-ccMCG-B system was then examined for SEM, EDX, XRD, setting time, compressive strength, injectability, pH change, BMP-2 release, in vitro as well as in vivo studies and further compared with CPC...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28529456/ethyl-acetate-fraction-of-huogu-formula-inhibits-adipogenic-differentiation-of-bone-marrow-stromal-cells-via-the-bmp-and-wnt-signaling-pathways
#12
Xiangying Kong, Xiaomin Li, Cun Zhang, Liuluan Zhu, Chunfang Liu, Qingxia Qin, Cuiling Liu, Qianqian Wang, Jia Zhu, Xuan Wu, Hongye Wan, Weiheng Chen, Na Lin
Elevated adipogenesis of bone marrow stromal cells (BMSCs) is closely associated with non-traumatic osteonecrosis of femoral head (ONFH). Our previous studies have shown that Huogu (HG) formula was effective both in clinic experience and experimental ONFH. How HG impacts the differentiation of BMSCs and what is the underlying molecular mechanism remain largely unknown. Our results showed that ethyl acetate extract of HG (HGE) significantly decreased the adipocyte differentiation as determined by oil red staining, while slightly increased the ALP activity...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28528141/portland-cement-induces-human-periodontal-ligament-cells-to-differentiate-by-upregulating-mir-146a
#13
Min-Ching Wang, Li-Yin Yeh, Wen-Yu Shih, Wan-Chun Li, Kuo-Wei Chang, Shu-Chun Lin
BACKGROUND/PURPOSE: Bioaggregates such as Portland cement (PC) can be an economical alternative for mineral trioxide aggregate (MTA) with additional benefit of less discoloration. MTA has been known to induce differentiations of several dental cells. MicroRNAs are important regulators of biological processes, including differentiation, physiologic homeostasis, and disease progression. This study is to explore how PC enhances the differentiation of periodontal ligament (PDL) cells in microRNAs level...
May 18, 2017: Journal of the Formosan Medical Association, Taiwan Yi Zhi
https://www.readbyqxmd.com/read/28527661/modulation-of-adipocyte-function-by-the-tgf-%C3%AE-family
#14
Yuhang Qiao, Shozo Tomonaga, Masashi Suenaga, Tohru Matsui, Masayuki Funaba
Members of the transforming growth factor-β (TGF-β) family are known to regulate the commitment of mesenchymal stem cells to an adipocyte lineage and the differentiation of preadipocytes to adipocytes. However, the roles of TGF-β family members in mature adipocytes are not known. The present study explored the modulation of cell function in 3T3-L1 adipocytes treated with the TGF-β family members TGF-β1, activin A, activin B, bone morphogenetic protein (BMP) 4, and BMP7. These TGF-β family members modulated the expression of some adipokines; activin A upregulated leptin expression, and activin B downregulated the expression of plasminogen activator inhibitor-1...
May 17, 2017: Cytokine
https://www.readbyqxmd.com/read/28527192/in-vitro-evaluation-of-an-injectable-biphasic-calcium-phosphate-bcp-carrier-system-combined-with-recombinant-human-bone-morphogenetic-protein-rhbmp-9
#15
Masako Fujioka-Kobayashi, Benoit Schaller, Yufeng Zhang, Benjamin E Pippenger, Richard J Miron
Bone morphogenetic protein 9 (BMP9) has previously been characterized as the strongest osteoinductive growth factor among the BMP family. The aim of the present study was to evaluate the possibility of combining rhBMP9 with an injectable biphasic calcium phosphate (I-BCP, maxresorb inject®), since I-BCP is an easy to handle biomaterial with ideal properties for bone augmentation procedures. The adsorption potential of rhBMP9 as well as the cell behavior of bone stromal ST2 cells were investigated on cell viability, adhesion, proliferation and osteogenic differentiation for I-BCP combined with/without rhBMP9 in vitro...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28525525/morphological-observation-on-critical-sized-cranial-defect-repaired-by-icariin-and-autologous-concentrate-growth-factors-in-rabbits
#16
Gang Li, Jie Wang, Guiyun Ren, Fuliang Hao, Yanning Zhang, Peikai Shi, Xiao Liu, Fusheng Dong
BACKGROUND Morphological changes repaired by icariin and autologous concentrate growth factors (ACGF) in critical-sized cranial defect were observed and their promoting effects were investigated. MATERIAL AND METHODS Seventy-two New Zealand white rabbits weighing 1.8~2.0kg were used to build a critical-sized cranial defect model and were randomly divided into 3 groups. X-ray, HE staining, general and histological observation, and immunohistochemistry were used to describe the changes caused by normal saline, icariin, and ACGF...
May 19, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28523267/a-novel-role-for-the-bmp-antagonist-noggin-in-sensitizing-cells-to-non-canonical-wnt-5a-ror2-disheveled-pathway-activation
#17
Ondrej Bernatik, Tomasz Radaszkiewicz, Martin Behal, Zankruti Dave, Florian Witte, Annika Mahl, Nicole H Cernohorsky, Pavel Krejci, Sigmar Stricker, Vitezslav Bryja
Mammalian limb development is driven by the integrative input from several signaling pathways; a failure to receive or a misinterpretation of these signals results in skeletal defects. The brachydactylies, a group of overlapping inherited human hand malformation syndromes, are mainly caused by mutations in BMP signaling pathway components. Two closely related forms, Brachydactyly type B2 (BDB2) and BDB1 are caused by mutations in the BMP antagonist Noggin (NOG) and the atypical receptor tyrosine kinase ROR2 that acts as a receptor in the non-canonical Wnt pathway...
2017: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/28522681/endothelial-nox1-oxidase-assembly-in-human-pulmonary-arterial-hypertension-driver-of-gremlin1-mediated-proliferation
#18
Imad Al Ghouleh, Sanghamitra Sahoo, Daniel N Meijles, Jefferson H Amaral, Daniel S de Jesus, John Sembrat, Mauricio Rojas, Dmitry A Goncharov, Elena A Goncharova, Patrick J Pagano
Background: Pulmonary arterial hypertension (PAH) is a rapidly degenerating and devastating disease of increased pulmonary vessel resistance leading to right heart failure. Palliative modalities remain limited despite recent endeavors to investigate the mechanisms underlying increased pulmonary vascular resistance, i.e. aberrant vascular remodeling and occlusion. However, little is known of the molecular mechanisms responsible for endothelial proliferation, a root cause of PAH-associated vascular remodeling...
May 18, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28522401/comparison-of-the-osteogenesis-and-fusion-rates-between-activin-a-bmp-2-chimera-ab204-and-rhbmp-2-in-a-beagle-s-posterolateral-lumbar-spine-model
#19
Guang Bin Zheng, Byung-Hak Yoon, Jae Hyup Lee
BACKGROUND CONTEXT: Activin A/BMP-2 chimera (AB204) could promote bone healing more effectively than recombinant bone morphogenetic protein 2 (rhBMP-2) with much lower dose in rodent model, but there is no report about the effectiveness of AB204 in a large animal model. PURPOSE: The purpose of this study was to compare the osteogenesis and fusion rate between AB204 and rhBMP-2 using biphasic calcium phosphate (BCP) as a carrier in beagle's posterolateral lumbar fusion model...
May 15, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28521202/dual-growth-factor-loaded-nonmulberry-silk-fibroin-carbon-nanofiber-composite-3d-scaffolds-for-in%C3%A2-vitro-and-in%C3%A2-vivo-bone-regeneration
#20
Deboki Naskar, Ananta K Ghosh, Mahitosh Mandal, Piyali Das, Samit K Nandi, Subhas C Kundu
In recent years the potential application of nanocomposite biomaterials in tissue engineering field is gaining importance because of the combined features of all the individual components. A bottom-up approach is acquired in this study to recreate the bone microenvironment. The regenerated silk protein fibroin obtained from nonmulberry tropical tasar Antheraea mylitta species is reinforced with functionalized carbon nano fiber (CNF) and the composite sponges are fabricated using facile green aqueous based method...
May 10, 2017: Biomaterials
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