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Giuseppe Criscenti, Carmelo De Maria, Alessia Longoni, Clemens A van Blitterswijk, Hugo A M Fernandes, Giovanni Vozzi, Lorenzo Moroni
The fabrication of bioactive scaffolds able to mimic the in vivo cellular microenvironment is a challenge for regenerative medicine. The creation of sites for the selective binding of specific endogenous proteins represents an attractive strategy to fabricate scaffolds able to elicit specific cell response. Here, electrospinning (ESP) and soft-molecular imprinting (soft-MI) techniques were combined to fabricate a soft-molecular imprinted electrospun bioactive scaffold (SMIES) for tissue regeneration. Scaffolds functionalized using different proteins and growth factors (GFs) arranged onto the surface were designed, fabricated and validated with different polyesters, demonstrating the versatility of the developed approach...
July 19, 2018: Biofabrication
Osny Ferreira Júnior, Etiene Andrade Munhoz, Jéssica de Fátima Segantin, Eduardo Sanches Gonçales, Paulo Sérgio Perri de Carvalho
OBJECTIVE: It is necessary to preserve height and thickness of the alveolar bone to facilitate rehabilitation with osteointegratable implants or simply to maintain bone integrity after extraction. Biomaterials associated with resorbable or non-resorbable membranes, when placed in the region of the socket, may contribute to avoid this unwanted reabsorption. OBJECTIVE: The objective of this study was to evaluate the distance of the crest of alveolar ridge to the cementoenamel junction (CEJ) of the lower second molars and the bone density of the third molar socket filled with Gen-Tech®, 5 years after an exodontia using cone beam computed tomography (CBCT) to visualize the central region of the sockets, without overlapping of the buccal and lingual cortical bones...
July 16, 2018: Journal of Applied Oral Science: Revista FOB
Gaoke Feng, Chaoshi Qin, Xin Yi, Jinggang Xia, Jingjing Chen, Xiyu Chen, Tao Chen, Xuejun Jiang
To study the effect of novel bioresorbable scaffold composed of poly-L-lactic acid (PLLA) and amorphous calcium phosphate (ACP) nanoparticles on inflammation and calcification of surrounding tissues after implantation. Ninety six PLLA/ACP scaffolds and 96 PLLA scaffolds were randomly implanted in the back muscle tissue of 48 SD rats. At the 1st, 2nd, 4th, and 12th weeks after implantation, the calcium, phosphorus, and alkaline phosphatase levels in the blood serum and the contents of calcium and alkaline phosphatase in the tissue surrounding the scaffolds were measured...
July 17, 2018: Journal of Materials Science. Materials in Medicine
Yongsun Kim, Byung-Jae Kang, Wan Hee Kim, Hui-Suk Yun, Oh-Kyeong Kweon
The aim of this study was to investigate the in vitro osteogenic capacity of bone morphogenetic protein 7 (BMP-7) overexpressing adipose-derived (Ad-) mesenchymal stem cells (MSCs) sheets (BMP-7-CS). In addition, BMP-7-CS were transplanted into critical-sized bone defects and osteogenesis was assessed. BMP-7 gene expressing lentivirus particles were transduced into Ad-MSCs. BMP-7, at the mRNA and protein level, was up-regulated in BMP-7-MSCs compared to expression in Ad-MSCs. Osteogenic and vascular-related gene expressions were up-regulated in BMP-7-CS compared to Ad-MSCs and Ad-MSC sheets...
July 17, 2018: International Journal of Molecular Sciences
Nahoko Iwata, Toru Hasegawa, Shiho Fujita, Satoko Nagao, Yasuhiro Nakano, Takahiro Nada, Yuki Nishiyama, Takeshi Hosoya, Fumio Otsuka
In the present study, we studied the effects of metformin and its interactions with the actions of bone morphogenetic proteins (BMPs) on ovarian steroidogenesis. It was revealed that metformin treatment enhanced progesterone production by human granulosa KGN cells and rat primary granulosa cells induced by forskolin and FSH, respectively. In human granulosa cells, it was found that metformin treatment suppressed phosphorylation of Smad1/5/9 activated by BMP-15 compared with that induced by other BMP ligands...
July 12, 2018: Biochemical and Biophysical Research Communications
Shu Li, Hongying Dai, Yong He, Shusheng Peng, Tianjin Zhu, Yang Wu, Chenwei Li, Ke Wang
Bone morphogenetic protein 9 (BMP9) is a member of the BMP family, which is involved in the regulation of tumor biogenesis, development and metastasis. The present study aimed to investigate whether BMP9 inhibits the growth of MDA‑MB‑231 breast cancer cells via the phosphoinositide 3‑kinase (PI3K)/Akt signaling pathway. It was shown that the expression level of BMP9 was significantly decreased, while that of phosphorylated Akt (p‑Akt) was markedly increased in breast cancer tissues compared with these levels in the normal adjacent tissues...
July 13, 2018: Oncology Reports
Weiwei Shen, Hailin Pang, Bo Xin, Lian Duan, Lili Liu, Helong Zhang
Small-cell lung cancer (SCLC) is typically fatal if untreated. It is characterized by early and widespread metastases, and has the ability to rapidly develop resistance to chemotherapy. Bone morphogenetic protein 7 (BMP7), a member of the BMP family of signaling molecules, has been implicated in various types of cancer, particularly prostate cancer and breast cancer. However, there is little knowledge of the function of BMP7 in SCLC. The aim of the present study was to investigate the biological function of recombinant human (rh)BMP7 on SCLC cells and the underlying molecular basis for this regulatory mechanism...
July 4, 2018: International Journal of Oncology
Adam Kamiński, Anna Bogacz, Izabela Uzar, Bogusław Czerny
OBJECTIVE: Osteoporosis is a civilization disease, in which the dominant symptoms are the loss of bone mass and disturbances in bone structure. Gremlin-2 is one of the BMP (bone morphogenetic proteins) antagonists and participate in osteogenesis and osteoblast differentiation. The aim of the study was to analyze whether the GREM2 gene polymorphism is significantly more common in postmenopausal women than in healthy women and whether it is a predisposing factor for the osteoporosis development...
July 9, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Fumio Otsuka
Background: Melatonin regulates circadian and seasonal rhythms and the activities of hormones and cytokines that are expressed in various tissues, including the ovary, in which melatonin receptors are expressed. In the ovary, follicular growth occurs as a result of complex interactions between pituitary gonadotropins and autocrine and paracrine factors, including bone morphogenetic proteins (BMPs) that are expressed in the ovary. Methods: The effects of melatonin and BMPs on steroidogenesis were examined by using the primary cultures of rat granulosa cells...
July 2018: Reproductive Medicine and Biology
Bum Sik Tae, Seok Cho, Hyun Cheol Kim, Cheol Hwan Kim, Seok Ho Kang, Jeong Gu Lee, Je Jong Kim, Hong Seok Park, Jun Cheon, Mi Mi Oh, Sung Gu Kang
We evaluated the prognostic value of BMP-2 expression in prostate cancer tissue via immunohistochemistry in prostate cancer patients. From July 2007 to August 2010, radical prostatectomy specimens from 90 patients with clinically localized prostate cancer (mean age, 62.7 years, mean follow-up 90.4 months) were assessed for BMP-2 expression using immunohistochemistry. We used stepwise multivariate Cox regression models stratified by study to assess the independent effects of the predictive factors and estimated hazard ratios (HRs)...
July 16, 2018: Scientific Reports
M Principe, M Chanal, V Karam, A Wierinckx, I Mikaélian, R Gadet, C Auger, V Raverot, E Jouanneau, A Vasiljevic, A Hennino, G Raverot, P Bertolino
Prolactinoma represents the most frequent hormone-secreting pituitary tumours. These tumours appear in a benign form, but some of them can reach an invasive and aggressive stage through an unknown mechanism. Discovering markers to identify prolactinoma proliferative and invading character is therefore crucial to develop new diagnostic/prognostic strategies. Interestingly, members of the TGFβ-Activin/BMP signalling pathways have emerged as important actors of pituitary development and adult function, but their role in prolactinomas remains to be precisely determined...
September 2018: Endocrine-related Cancer
Hyang-Yu Kim, Sun-Young Park, Se-Young Choung
Myricetin is a flavonoid that found in berries, onions, and red grapes. It has been reported to have various pharmacological effects such as anti-inflammation, anti-oxidant and anti-cancer activities. However, the underlying mechanisms of myricetin on osteogenic differentiation remain unknown in human periodontal ligament stem cells (hPDLSCs). In this study, we investigated the ability of myricetin to increase osteogenic differentiation and its underlying molecular mechanisms. Myricetin significantly increased cell proliferation, alkaline phosphatase (ALP...
July 13, 2018: European Journal of Pharmacology
Yilin Jie, Xiaoyun Li, Zhaoxia Cai, Meihu Ma, Yongguo Jin, Dong Uk Ahn, Xi Huang
This study investigated the effects of phosvitin (PV), one of the major proteins from egg yolk, with different degree of phosphorylation on the physiology of an osteoblast (MC3T3-E1) cell line. The proliferation and differentiation of MC3T3-E1 were analyzed using the CCK-8 and the alkaline phosphatase (ALP) assay, respectively. The effect of PV on the mineralization of MC3T3-E1 was monitored using the Alizarin-red staining. PV at 100 μg/mL increased the ALP activity by 145% of the control after 7 days of incubation...
July 13, 2018: International Journal of Biological Macromolecules
Daheui Choi, Jin Chan Park, Ha Na Lee, Ji-Hoi Moon, Hyo-Won Ahn, Kwangyong Park, Jinkee Hong
Chalcone derivatives have been investigated for therapeutic agent such as anticancer, antioxidant and anti-inflammatory fields. In this study, we also have synthesized 4 different type of chalcone derivatives and demonstrated in vitro bioactivities. We divided into 2 groups of chalcones on the basis of similar substituents at aromatic rings and tested cell viability and proliferation potentials, indicating that methoxy substituent on A ring could enhance cytotoxicity and anti-proliferation potential depending on chalcone concentration...
July 16, 2018: Molecular Pharmaceutics
Che Lin Kim, Mi Yeong Jung, Young Sik Kim, Ju Woong Jang, Gyun Min Lee
Endocytic regulation serves a critical role in modulating the extracellular level of signaling molecules such as bone morphogenetic proteins (BMPs). Unfortunately, endocytosis may result in poor yields of recombinant human BMP-4 (rhBMP-4) from Chinese hamster ovary (CHO) cell cultures. When rhBMP-4 was incubated with CHO cells, rhBMP-4 was actively internalized into cells. Cell surface bound-heparan sulfate proteoglycans (HSPGs) served as the major receptors for rhBMP-4 internalization. Removal of cell surface heparan sulfate (HS) by heparinases or reduction of HSPG synthesis by knockdown of xylosyltransferase2 (xylt2) in CHO cells decreased internalization of rhBMP-4...
July 16, 2018: Biotechnology and Bioengineering
Geir Bjorklund, Erik Svanberg, Maryam Dadar, David J Card, Salvatore Chirumbolo, Dominic J Harrington, Jan Aaseth
Matrix Gla protein (MGP) is a vitamin K-dependent protein, which is synthesized in bone and many further mesenchymal cells, which is also highly expressed by vascular smooth 1muscle cells (VSMCs) and chondrocytes. Numerous studies have confirmed that MGP acts as a calcification-inhibitor although the mechanism of action is still not fully understood. The modulation of tissue calcification by MGP is potentially regulated in several ways including direct inhibition of calcium-phosphate precipitation, the formation of matrix vesicles (MVs), the formation of apoptotic bodies (ABs), and trans-differentiation of VSMCs...
July 15, 2018: Current Medicinal Chemistry
Wen Zhang, Rodolfo E De La Vega, Michael J Coenen, Carlos J Peniche Silva, Manish Aneja, Christian Plank, Martijn van Griensven, Christopher Evans, Elizabeth R Balmayor
The first therapeutic application of mRNA was suggested over two decades ago. However, its application was constrained by the ability of mRNA to activate the innate immune response, cytotoxicity and poor potency. We and others recently demonstrated that these undesirable properties of mRNA may be overcome by alterating its structure. In this study, we developed a new chemically modified mRNA coding for BMP-2 with improved osteogenic features. To develop this new construct, we removed from the mRNA sequence the following undesirable elements: an upstream ORF in the 5'UTR and a polyadenylation element together with an AU-rich tract in the 3'UTR...
July 16, 2018: Tissue Engineering. Part A
Lina Wang, Qin Ding, Li Zhao, Yu Pan, Zhengxia Song, Yanan Qin, Xuebing Yan
The aim of the present study was to investigate the expression of bone morphogenetic protein-7 (BMP-7), gremlin, and p-Smad/1/5/8 in carcinomatous and para-carcinoma tissue specimens from patients with hepatocellular carcinoma (HCC). The association of serum BMP-7 levels with clinicopathological parameters was examined to assess its relevance as a clinical biomarker for HCC. A total of 27 patients with HCC and 7 healthy subjects were included. Gene expression levels of BMP-7 and p-Smad1/5/8 were examined by reverse transcription-quantitative polymerase chain reaction...
August 2018: Oncology Letters
Pragati Kushwaha, Ashish Kumar Tripathi, Sampa Gupta, Priyanka Kothari, Akanksha Upadhyay, Naseer Ahmad, Tanuj Sharma, M I Siddiqi, Ritu Trivedi, Koneni V Sashidhara
Twenty-four novel benzofuran-pyran derivatives were synthesized and evaluated for their anti-osteoporotic activity in primary cultures of rat calvarial osteoblasts in vitro. Among all the compounds screened for the alkaline phosphatase activity, three compounds 4e, 4j and 4k showed potent activity at picomolar concentrations in osteoblast differentiating stimulation. Additionally, these compounds were found effective in mineralization, assessed by alizarin red-S staining assay. Compounds were again validated through a series of other in vitro experiments...
June 28, 2018: European Journal of Medicinal Chemistry
Gry Hulsart-Billström, Ram Kumar Selvaraju, Sergio Estrada, Mark Lubberink, Veronika Asplund, Kristoffer Bergman, Richard Marsell, Sune Larsson, Gunnar Antoni
Bone morphogenetic proteins (BMP's) are vital for bone and cartilage formation, where bone morphogenetic protein-2 (BMP-2) is acknowledged as a growth factor in osteoblast differentiation. However, uncontrolled delivery may result in adverse clinical effects. In this study we investigated the possibility for longitudinal and non-invasive monitoring of implanted [125 I]BMP-2 retention and its relation to ossification at the site of implantation. A unilateral critically sized femoral defect was produced in the left limb of rats while the right femur was retained intact as a paired reference control...
July 10, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
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