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https://www.readbyqxmd.com/read/28215046/genome-resolved-metagenomics-of-a-bioremediation-system-for-degradation-of-thiocyanate-in-mine-water-containing-suspended-solid-tailings
#1
Sumayah F Rahman, Rose S Kantor, Robert Huddy, Brian C Thomas, Andries W van Zyl, Susan T L Harrison, Jillian F Banfield
Thiocyanate (SCN(-) ) is a toxic compound that forms when cyanide (CN(-) ), used to recover gold, reacts with sulfur species. SCN(-) -degrading microbial communities have been studied, using bioreactors fed synthetic wastewater. The inclusion of suspended solids in the form of mineral tailings, during the development of the acclimatized microbial consortium, led to the selection of an active planktonic microbial community. Preliminary analysis of the community composition revealed reduced microbial diversity relative to the laboratory-based reactors operated without suspended solids...
February 19, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28214985/melatonin-alleviates-the-epilepsy-associated-impairments-in-hippocampal-ltp-and-spatial-learning-through-rescue-of-surface-glur2-expression-at-hippocampal-ca1-synapses
#2
Yue Ma, Xiaolong Sun, Juan Li, Ruihua Jia, Fang Yuan, Dong Wei, Wen Jiang
Epilepsy-associated cognitive impairment is common, and negatively impacts patients' quality of life. However, most antiepileptic drugs focus on the suppression of seizures, and fewer emphasize treatment of cognitive dysfunction. Melatonin, an indolamine synthesized primarily in the pineal grand, is reported to be neuroprotective against several central nervous system disorders. In this study, we investigated whether melatonin could reverse cognitive dysfunction in lithium-pilocarpine treated rats. Chronic treatment with melatonin (8 mg/kg daily for 15 days) after induction of status epilepticus significantly alleviated seizure severity, reduced neuronal death in the CA1 region of the hippocampus, improved spatial learning (as measured by the Morris water maze test), and reversed LTP impairments, compared to vehicle treatment...
February 18, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28214984/dl-3-n-butylphthalide-nbp-provides-neuroprotection-in-the-mice-models-after-traumatic-brain-injury-via-nrf2-are-signaling-pathway
#3
Zhengwei Liu, Handong Wang, Xiaofeng Shi, Liwen Li, Mengliang Zhou, Hui Ding, Youqing Yang, Xiang Li, Ke Ding
The present study was aimed to evaluate the neuroprotective effects of NBP in the mice models of TBI, as well as the possible role of Nrf2-ARE pathways in the assumptive neuroprotection. In mice,a modified Marmarou's weight-drop model was employed to induce TBI. ICR mice were randomly assigned to four experimental groups: sham, TBI, TBI+vehicle(V) and TBI+NBP. NBP (100 mg/kg) was administered via an intraperitoneal (i.p.) injection at 1 h following TBI. The administration of NBP significantly ameliorated the effects of the brain injury, including neurological deficits, brain water content, and cortical neuronal apoptosis...
February 18, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28214941/monitoring-water-pollution-levels-in-wadi-hanifa-riyadh-saudi-arabia-and-its-public-health-implications
#4
Bushra Ahmed Al-Hammad, Magda Magdy Abd El-Salam
This study was performed to evaluate the levels of metals in the fish caught from Wadi Hanifa's main basin over four seasons, determine the potential fitness of the fish for human consumption, and evaluate the overall water quality after enforcement of local standards. The physical and chemical parameters from a total of 192 water samples were tested using standard methods. Additionally, a total of 48 fish samples were analyzed for heavy metal concentrations. Mean values for basic water quality parameters of COD, PO4(-3), NH3(-)-N, and NO3(-)-N exceeded Saudi standards in all seasons...
February 18, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28214939/selective-determination-of-sulfonamides-from-environmental-water-based-on-magnetic-surface-molecularly-imprinting-technology
#5
Yang Xu, Qi Zhao, Liyan Jiang, Zhengqiang Li, Yanhua Chen, Lan Ding
In the study, a simple and selective method based on magnetic separation technology is presented for the extraction of sulfonamides (SAs) from environmental water, followed by liquid chromatography-tandem mass spectrometry. In this method, magnetic surface molecularly imprinted polymers (Fe3O4@SiO2@MIPs) with super-paramagnetic property and high selectivity toward SAs were developed as magnetic adsorbents. The Fe3O4@SiO2@MIPs were then applied to the selective extraction of SAs from environmental water. The extraction and enrichment were accomplished simultaneously in a single step by simply stirring the mixture of adsorbents and water samples...
February 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28214919/grapevine-acclimation-to-water-deficit-the-adjustment-of-stomatal-and-hydraulic-conductance-differs-from-petiole-embolism-vulnerability
#6
Uri Hochberg, Andrea Giulia Bonel, Rakefet David-Schwartz, Asfaw Degu, Aaron Fait, Hervé Cochard, Enrico Peterlunger, Jose Carlos Herrera
Drought-acclimated vines maintained higher gas exchange compared to irrigated controls under water deficit; this effect is associated with modified leaf turgor but not with improved petiole vulnerability to cavitation. A key feature for the prosperity of plants under changing environments is the plasticity of their hydraulic system. In the present research we studied the hydraulic regulation in grapevines (Vitis vinifera L.) that were first acclimated for 39 days to well-watered (WW), sustained water deficit (SD), or transient-cycles of dehydration-rehydration-water deficit (TD) conditions, and then subjected to varying degrees of drought...
February 18, 2017: Planta
https://www.readbyqxmd.com/read/28214907/a-pilot-study-of-bacterial-regrowth-in-water-pipelines
#7
Laura Mancini, Stefania Marcheggiani, Cinzia Grasso, Cristina Romanelli, Antonio Mistretta, Marina Marranzano
Bacterial regrowth in water distribution systems results in deterioration of bacteriological quality of drinking water, as well as accelerated corrosion of the pipelines. The aim of the present study was to analyze the phenomena of colonization and bacterial regrowth in source water and in the water distribution systems of three distribution networks in the province of Catania (Sicily, Italy). The BART™ (Biological Activity Reaction Test) method was used, which is also capable of determining the potential aggressiveness of microbial species in water...
November 2016: Igiene e Sanità Pubblica
https://www.readbyqxmd.com/read/28214838/the-role-of-hippocampal-structural-synaptic-plasticity-in-repetitive-transcranial-magnetic-stimulation-to-improve-cognitive-function-in-male-samp8-mice
#8
Jiang Ma, Jinhua Wang, Chaonan Lv, Jingjuan Pang, Bing Han, Mingwei Wang, Yuan Gen
BACKGROUND: Repetitive transcranial magnetic stimulation (rTMS) has been used to improve cognitive function, but the stimulation protocols are variable and the underlying mechanism is unclear. Therefore, we intend to examine whether 5Hz rTMS with 30% maximum output could improve cognitive functions in senescence-accelerated-prone mouse 8 (SAMP8) through changing synaptic plasticity. METHODS: SAMP8 and senescence-accelerated-prone mouse/resistant 1 (SAMR1) (7-month old male) were randomly divided into 3 groups: SMAP8 rTMS group (P8-rTMS), SMAP8 sham-rTMS group (P8-sham), and SAMR1 sham-rTMS group (R1-sham)...
January 18, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28214828/role-for-target-of-rapamycin-mtor-signal-pathway-in-regulating-neuronal-injury-after-intracerebral-hemorrhage
#9
Jie-Ping Wang, Meng-Yu Zhang
BACKGROUND/AIMS: Mammalian target of rapamycin (mTOR) is a serine/threonine protein kinase and activation of its signal pathway plays an important role in regulating protein growth and synthesis as well as cell proliferation and survival. In the present study, we examined the contribution of mTOR signal and its downstream products to brain injuries induced by intracerebral hemorrhage (ICH) in rats. METHODS: Western Blot analysis was employed to examine the protein expression of mTOR and its downstream pathway and Caspase-3...
January 18, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28214776/the-metabolic-impact-of-extracellular-nitrite-on-aerobic-metabolism-of-paracoccus-denitrificans
#10
K R Hartop, M J Sullivan, G Giannopoulos, A J Gates, P L Bond, Z Yuan, T A Clarke, G Rowley, D J Richardson
Nitrite, in equilibrium with free nitrous acid (FNA), can inhibit both aerobic and anaerobic growth of microbial communities through bactericidal activities that have considerable potential for control of microbial growth in a range of water systems. There has been much focus on the effect of nitrite/FNA on anaerobic metabolism and so, to enhance understanding of the metabolic impact of nitrite/FNA on aerobic metabolism, a study was undertaken with a model denitrifying bacterium Paracoccus denitrificans PD1222...
February 7, 2017: Water Research
https://www.readbyqxmd.com/read/28214747/immunorecognition-magnetic-supports-for-the-development-of-an-electrochemical-immunoassay-for-azaspiracid-detection-in-mussels
#11
Sandra Leonardo, Maria Rambla-Alegre, Ingunn A Samdal, Christopher O Miles, Jane Kilcoyne, Jorge Diogène, Ciara K O'Sullivan, Mònica Campàs
As azaspiracids (AZAs) are being reported from the coastal waters of an increasing number of countries on a global scale, the need for rapid, simple and cost-effective methods to detect these marine toxins and protect seafood consumers' health is becoming evident. A magnetic bead (MB)-based direct immunoassay for the detection of AZAs, using protein G-coated MBs as supports for antibody immobilisation and peroxidase-labelled AZA as a tracer is detailed. A colorimetric approach was first developed to optimise the experimental parameters and establish the cross-reactivity factors for AZA-1-10...
February 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28214744/tuning-of-the-selectivity-of-fluorescent-peptidyl-bioprobe-using-aggregation-induced-emission-for-heavy-metal-ions-by-buffering-agents-in-100-aqueous-solutions
#12
Lok Nath Neupane, Gi Won Hwang, Keun-Hyeung Lee
Smart fluorescent probes of which the detection of specific target molecules can be controlled are attracting remarkable interest. A fluorescent peptidyl bioprobe (1) was rationally synthesized by conjugating tetraphenylethylene, an aggregation-induced emission (AIE) fluorophore with a peptide receptor (AspHis) that acted as hard and intermediate bases. The selective detection of 1 for specific metal ion in 100% aqueous solutions was controlled by the buffering agents with the chelate effect without the change of pH...
February 3, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28214729/improved-tolerance-to-transplanting-injury-and-chilling-stress-in-rice-seedlings-treated-with-orysastrobin
#13
Naoto Takahashi, Yukari Sunohara, Masami Fujiwara, Hiroshi Matsumoto
In addition to their fungicidal activity, strobilurin-type fungicides are reported to show enhancing effects on crop growth and yield. Previous studies suggested that the fungicide has a mitigating effect on abiotic stresses. However, there are few reports about growth enhancement through abiotic stress alleviation by strobilurin-type fungicides, but the mechanism of action of the growth enhancement is still not clear. The present study revealed that orysastrobin enhanced rice seedling growth after root cutting injury and chilling stress...
February 7, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28214721/waterborne-transmission-of-protozoan-parasites-review-of-worldwide-outbreaks-an-update-2011-2016
#14
REVIEW
Artemis Efstratiou, Jerry E Ongerth, Panagiotis Karanis
This review provides a comprehensive update of worldwide waterborne parasitic protozoan outbreaks that occurred with reports published since previous reviews largely between January 2011 and December 2016. At least 381 outbreaks attributed to waterborne transmission of parasitic protozoa were documented during this time period. The nearly half (49%) of reports occurred in New Zealand, 41% of the outbreaks in North America and 9% in Europe. The most common etiological agent was Cryptosporidium spp., reported in 63% (239) of the outbreaks, while Giardia spp...
January 25, 2017: Water Research
https://www.readbyqxmd.com/read/28214713/comparative-analysis-of-metagenomes-of-italian-top-soil-improvers
#15
Federica Gigliucci, Gianfranco Brambilla, Rosangela Tozzoli, Valeria Michelacci, Stefano Morabito
Biosolids originating from Municipal Waste Water Treatment Plants are proposed as top soil improvers (TSI) for their beneficial input of organic carbon on agriculture lands. Their use to amend soil is controversial, as it may lead to the presence of emerging hazards of anthropogenic or animal origin in the environment devoted to food production. In this study, we used a shotgun metagenomics sequencing as a tool to perform a characterization of the hazards related with the TSIs. The samples showed the presence of many virulence genes associated to different diarrheagenic E...
February 16, 2017: Environmental Research
https://www.readbyqxmd.com/read/28214698/value-adding-conversion-and-volume-reduction-of-sewage-sludge-by-anaerobic-co-digestion-with-crude-glycerol
#16
Kensuke Kurahashi, Chie Kimura, You Fujimoto, Hayato Tokumoto
In this study, conversion of sewage sludge to biogas by anaerobic co-digestion with crude glycerol was examined. When 0.126g/L crude glycerol was added to the reactor, only methane was produced. Upon addition of 5.04g/L crude glycerol, hydrogen production occurred, and a significant amount of 1,3-propanediol (1,3-PDO) was generated in the liquid phase. On day 6, the dry weight was largely composed of organic acids (48%) and 1,3-PDO (17%), which are water-soluble. Degradation of 1,3-PDO was very slow, which is advantageous for recovery...
February 7, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28214685/role-of-laxative-and-antioxidant-properties-of-malva-sylvestris-leaves-in-constipation-treatment
#17
Mohamed-Amine Jabri, Dalanda Wannes, Najla Hajji, Mohsen Sakly, Lamjed Marzouki, Hichem Sebai
INTRODUCTION: Mallaw (Malva sylvestris L.) is a medicinal plant who is traditionally being used as an antiulcer, laxative and anti-hemorrhoid, besides of its culinary use as a food in Tunisian cuisine. The present study was carried out to evaluate the protective effect of Malva sylvestris aqueous extract (MSAE) on constipation- induced by loperamide in male Wistar rats. METHODS: Rats were divided into six groups of six animals each: Control, Loperamide (LOP), LOP+ yohimbine (2mg/kg b...
February 16, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28214672/evaluation-of-molecular-chaperone-drug-function-regorafenib-and-%C3%AE-cyclodextrins
#18
Xiurong Hu, Mengying Sun, Yang Li, Guping Tang
Regorafenib (RG) was an oral multi-kinase inhibitor with poor water solubility. In order to enhance the drug's solubility, dissolution and bioavailability, the binary molecular chaperone drug between RG and β-cyclodetrin (β-CD) had prepared by co-crystallization. The structure of RG-β-CD was characterized by thermal analysis, powder X-ray diffraction, infrared spectroscopy and nuclear magnetic resonance. Phase-solubility study revealed the higher solubility and complexing ability of β-CDwith RG.The solubility and dissolution of RG-β-CD was significantly enhanced in pH 1...
February 7, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28214648/toxicity-degradation-and-metabolic-fate-of-ibuprofen-on-freshwater-diatom-navicula-sp
#19
Tengda Ding, Mengting Yang, Junmin Zhang, Bo Yang, Kunde Lin, Juying Li, Jay Gan
Ibuprofen (IBU) is one of the most widely used and frequently detected human pharmaceuticals in aquatic environment. However, the toxicity of IBU on diatom and its fate remain still unkown. In the present study, the toxicity of IBU on the diatom was evaluated by the algal growth rate, the chlorophyll-a and carotenoids contents. The degradation of IBU including in particular the potential for the formation of incomplete degradation products was also explored. Biochemical characteristics of Navicula sp. were significantly inhibited at IBU concentrations up to 50mgL(-1) after 10days of exposure...
February 6, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28214629/effects-of-metal-ion-replacement-on-pyrazinamidase-activity-a-quantum-mechanical-study
#20
Mahmoud Khadem-Maaref, Faramarz Mehrnejad, Arash Phirouznia
Pyrazinamidase (PZase), a metalloenzyme, is responsible for acidic modification of pyrazinamide (PZA), a drug used in tuberculosis treatment. The metal coordination site of the enzyme is able to coordinate various divalent metal cofactors. Previous experimental studies have demonstrated that metal ions, such as Co(2+), Mn(2+), and Zn(2+), are able to reactivate metal-depleted PZase, while others including Cu(2+), Fe(2+), and Mg(2+), cannot restore activity. In this study, we investigated binding of various metal ions to the metal coordination site (MCS) of the enzyme using quantum mechanical calculations...
February 2, 2017: Journal of Molecular Graphics & Modelling
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