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regenerative medicine with stem cells

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https://www.readbyqxmd.com/read/28925552/in-situ-bone-tissue-engineering-with-an-endogenous-stem-cell-mobilizer-and-osteoinductive-nanofibrous-polymeric-scaffolds
#1
Jong Seung Lee, Yoonhee Jin, Hyun-Ji Park, Kisuk Yang, Min Suk Lee, Hee Seok Yang, Seung-Woo Cho
Classical bone tissue engineering involves the use of culture-expanded cells and scaffolds to produce tissue constructs for transplantation. Despite promising results, clinical adoption of these constructs has been limited due to various drawbacks, including extensive cell expansion steps, low cell survival rate upon transplantation, and the possibility of immuno-rejection. To bypass the ex vivo cell culture and transplantation process, we exploited the regenerative capacity of the host by mobilizing endogenous stem cells to the site of injury...
September 19, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28925549/biomaterial-substrate-mediated-multicellular-spheroid-formation-and-their-applications-in-tissue-engineering
#2
REVIEW
Ting-Chen Tseng, Chui-Wei Wong, Fu-Yu Hsieh, Shan-Hui Hsu
Three-dimentional (3D) multicellular aggregates (spheroids), compared to the traditional 2D monolayer cultured cells, are physiologically more similar to the cells in vivo. So far there are various techniques to generate 3D spheroids. Spheroids obtained from different methods have already been applied to regenerative medicine or cancer research. Among the cell spheroids created by different methods, the substrate-derived spheroids and their forming mechanism are unique. This review focuses on the formation of biomaterial substrate-mediated multicellular spheroids and their applications in tissue engineering and tumor models...
September 19, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28922348/stem-cell-biology-and-regenerative-medicine-for-neonatal-lung-diseases
#3
REVIEW
Martin Kang, Bernard Thébaud
Lung diseases remain one of the main causes of morbidity and mortality in neonates. Cell therapy and regenerative medicine have the potential to revolutionize the management of life-threatening and debilitating lung diseases that currently lack effective treatments. Over the past decade, the repair capabilities of stem/progenitor cells has been harnessed to prevent/rescue lung damage in experimental neonatal lung diseases. Mesenchymal stromal cells and amnion epithelial cells exert pleiotropic effects and represent ideal therapeutic cells for bronchopulmonary dysplasia, a multifactorial disease...
September 18, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28918520/derivation-of-human-induced-pluripotent-stem-cell-ipsc-lines-and-mechanism-of-pluripotency-historical-perspective-and-recent-advances
#4
REVIEW
Arvind Chhabra
Derivation of human embryonic stem cell (hES) lines in 1998 was not only a major technological breakthrough in the field of regenerative medicine; it also triggered a passionate debate about the ethical issues associated with the utilization of human embryos for derivation of hESC lines. Successful derivation of induced pluripotent stem cell (iPS) lines from human somatic cells with defined reprogramming factors by Shinya Yamanaka`s group in 2007 was another breakthrough that generated enormous excitement and hope for the development of donor-specific personalized cell replacement therapies (CRT) without the ethical dilemma associated with it...
September 16, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28912894/genetically-encoded-photoactuators-and-photosensors-for-characterization-and-manipulation-of-pluripotent-stem-cells
#5
REVIEW
Jordan E Pomeroy, Hung X Nguyen, Brenton D Hoffman, Nenad Bursac
Our knowledge of pluripotent stem cell biology has advanced considerably in the past four decades, but it has yet to deliver on the great promise of regenerative medicine. The slow progress can be mainly attributed to our incomplete understanding of the complex biologic processes regulating the dynamic developmental pathways from pluripotency to fully-differentiated states of functional somatic cells. Much of the difficulty arises from our lack of specific tools to query, or manipulate, the molecular scale circuitry on both single-cell and organismal levels...
2017: Theranostics
https://www.readbyqxmd.com/read/28905449/lull-pgm-system-a-suitable-technique-to-improve-the-regenerative-potential-of-autologous-fat-grafting
#6
Paolo G Morselli, Federico A Giorgini, Claudia Pazzini, Claudio Muscari
Autologous fat grafting and methods of purification of harvested tissue have become one of the most current themes in regenerative medicine. The aim of this study was to evaluate the in vitro regenerative potential of abdomen lipoaspirates subjected to a combined washing-decantation purifying procedure, the Lull pgm System (Lull). Blood cells and stromal-vascular fraction (SVF) cells contained in the aspirates were investigated and compared with those obtained through more conventional fat-processing methods, i...
September 14, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28903899/the-application-of-next-generation-sequencing-techniques-in-studying-transcriptional-regulation-in-embryonic-stem-cells
#7
Ya-Jun Liu, Feng Zhang, Hong-de Liu, Xiao Sun
The mechanism of transcriptional regulation has been the focus of many studies in the post-genomic era. The development of sequencing-based technologies for chromatin profiling enables current researchers to experimentally measure chromatin properties. Moreover, many studies aim at annotating the state of the chromatin into broad categories based on observed chromatin features and/or DNA sequences, then associating the resultant distal regulatory regions with the correct target genes based on DNA sequences, and predicting the dependence of epigenetic features on genetic variation...
August 20, 2017: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/28903069/cell-reprogramming-therapeutic-potential-and-the-promise-of-rejuvenation-for-the-aging-brain
#8
REVIEW
Micaela López-León, Tiago F Outeiro, Rodolfo G Goya
Aging is associated with a progressive increase in the incidence of neurodegenerative diseases, with Alzheimer's (AD) and Parkinson's (PD) disease being the most conspicuous examples. Within this context, the absence of efficacious therapies for most age-related brain pathologies has increased the interest in regenerative medicine. In particular, cell reprogramming technologies have ushered in the era of personalized therapies that not only show a significant potential for the treatment of neurodegenerative diseases but also promise to make biological rejuvenation feasible...
September 10, 2017: Ageing Research Reviews
https://www.readbyqxmd.com/read/28902422/transplantation-of-non-cultured-stromal-vascular-fraction-cells-of-adipose-tissue-ameliorates-osteonecrosis-of-the-jaw-like-lesions-in-mice
#9
Shinichiro Kuroshima, Muneteru Sasaki, Kazunori Nakajima, Saki Tamaki, Hiroki Hayano, Takashi Sawase
The precise pathoetiology and effective treatment strategies for bisphosphonate-related osteonecrosis of the jaw (BRONJ) remain unknown. Transplantation of non-cultured stromal vascular fraction (SVF) cells has been demonstrated to be a useful method for regenerative medicine in place of stem cell therapy. This study investigated the effects of non-cultured SVF transplantation on tooth extraction socket healing in mice. Both chemotherapeutic/bisphosphonate combination therapy for 7 weeks and tooth extraction of maxillary first molars at 3 weeks after drug administration were performed using female C57BL/6J mice...
September 13, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28901712/engineering-concepts-in-stem-cell-research
#10
REVIEW
Karthikeyan Narayanan, Sachin Mishra, Satnam Singh, Ming Pei, Balazs Gulyas, Parasuraman Padmanabhan
The field of regenerative medicine integrates advancements made in stem cells, molecular biology, engineering and clinical methodologies. Stem cells serve as a fundamental ingredient for therapeutic application in regenerative medicine. Apart from stem cells, engineering concepts have equally contributed to the success of stem cell based applications in improving human health. The purpose of various engineering methodologies is to develop regenerative and preventive medicine to combat various diseases and deformities...
September 13, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28901500/cell-cycle-and-pluripotency-convergence-on-octamer%C3%A2-binding-transcription-factor-4-review
#11
Shiqi She, Qucheng Wei, Bo Kang, Ying-Jie Wang
Embryonic stem cells (ESCs) have unlimited expansion potential and the ability to differentiate into all somatic cell types for regenerative medicine and disease model studies. Octamer‑binding transcription factor 4 (OCT4), encoded by the POU domain, class 5, transcription factor 1 gene, is a transcription factor vital for maintaining ESC pluripotency and somatic reprogramming. Many studies have established that the cell cycle of ESCs is featured with an abbreviated G1 phase and a prolonged S phase. Changes in cell cycle dynamics are intimately associated with the state of ESC pluripotency, and manipulating cell‑cycle regulators could enable a controlled differentiation of ESCs...
September 13, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28900152/a-3d-magnetic-tissue-stretcher-for-remote-mechanical-control-of-embryonic-stem-cell-differentiation
#12
Vicard Du, Nathalie Luciani, Sophie Richard, Gaëtan Mary, Cyprien Gay, François Mazuel, Myriam Reffay, Philippe Menasché, Onnik Agbulut, Claire Wilhelm
The ability to create a 3D tissue structure from individual cells and then to stimulate it at will is a major goal for both the biophysics and regenerative medicine communities. Here we show an integrated set of magnetic techniques that meet this challenge using embryonic stem cells (ESCs). We assessed the impact of magnetic nanoparticles internalization on ESCs viability, proliferation, pluripotency and differentiation profiles. We developed magnetic attractors capable of aggregating the cells remotely into a 3D embryoid body...
September 12, 2017: Nature Communications
https://www.readbyqxmd.com/read/28898760/live-cell-imaging-of-mouse-intestinal-organoids-reveals-heterogeneity-in-their-oxygenation
#13
Irina A Okkelman, Tara Foley, Dmitri B Papkovsky, Ruslan I Dmitriev
Intestinal organoids are widely applied in stem cell research, regenerative medicine, toxicology, pharmacology, and host-microbe interactions research. The variability of oxidative metabolism for stem and differentiated cell types constituting organoid is known to be important but so far it has not been studied in details. Here, we report the use of live cell microscopy of oxygen via the phosphorescence lifetime imaging microscopy (PLIM) method to address the oxygenation and variability of aerobic metabolism of individual organoids in the culture...
September 3, 2017: Biomaterials
https://www.readbyqxmd.com/read/28895404/exosomes-of-human-mesenchymal-stem-stromal-medicinal-signaling-cells-a-review
#14
U Altanerova, J Jakubechova, V Repiska, C Altaner
In this review, we intend to explore the potential therapeutic effects of exosomes released from mesenchymal stem/stromal cells (MSCs). MSCs gained credibility as a therapeutic tool due to their potential to differentiate into many cell types like osteoblasts, chondrocytes, adipocytes, muscular, endothelial, cardiovascular, and neurogenic cells. They possess potent wound healing activity due to their immunosuppressive and anti-inflammatory properties. MSCs are tested in large number of clinical trials for treatment of diseases, which do not have adequate therapy at present...
September 12, 2017: Neoplasma
https://www.readbyqxmd.com/read/28894864/neural-stem-cell-proliferation-and-differentiation-in-the-conductive-pedot-ha-cs-gel-scaffold-for-neural-tissue-engineering
#15
Shuping Wang, Shui Guan, Jianqiang Xu, Wenfang Li, Dan Ge, Changkai Sun, Tianqing Liu, Xuehu Ma
Engineering scaffolds with excellent electro-activity is increasingly important in tissue engineering and regenerative medicine. Herein, conductive poly(3,4-ethylenedioxythiophene) doped with hyaluronic acid (PEDOT-HA) nanoparticles were firstly synthesized via chemical oxidant polymerization. A three-dimensional (3D) PEDOT-HA/Cs/Gel scaffold was then developed by introducing PEDOT-HA nanoparticles into a chitosan/gelatin (Cs/Gel) matrix. HA, as a bridge, not only was used as a dopant, but also combined PEDOT into the Cs/Gel via chemical crosslinking...
September 12, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28894288/altered-adipose-derived-stem-cell-characteristics-in-macrodactyly
#16
Xi Yang, Yongkang Jiang, Gang Han, Yuan Shi, Shengbo Zhou, Feng Ni, Bin Wang
Macrodactyly is a congenital disease characterized by aggressive overgrowth of adipose tissue in digits or limbs frequently accompanied with hyperostosis and nerve enlargement; its pathological mechanism is poorly understood. Adipose-derived stem cells (ASCs) have been extensively studied in tissue engineering and regenerative medicine as an ideal alternative substitute for bone marrow-derived mesenchymal stem cells (BM-MSCs), but their pathological role is largely unknown. In this study, ASCs from macrodactyly adipose tissues (Mac-ASCs) were isolated and compared to ASCs derived from the normal abdominal subcutaneous adipose tissue (Sat-ASCs) for cell morphology, surface marker expression, proliferation rate, and tri-lineage differentiation potential...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28888046/gap-junctional-intercellular-communication-in-adipose-derived-stromal-stem-cells-is-cell-density-dependent-and-positively-impacts-adipogenic-differentiation
#17
Wiesner Miriam, Berberich Oliver, Hoefner Christiane, Blunk Torsten, Bauer-Kreisel Petra
Adipose-derived stromal/stem cells (ASCs) represent a widely used cell source with multi-lineage differentiation capacity in approaches for tissue engineering and regenerative medicine. Despite the multitude of literature on their differentiation capacity, little is reported about the physiological properties contributing to and controlling the process of lineage differentiation. Direct intercellular communication between adjacent cells via gap junctions has been shown to modulate differentiation processes in other cell types, with connexin 43 (Cx43) being the most abundant isoform of the gap junction-forming connexins...
September 9, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28887108/new-approach-for-the-derivation-of-melanocytes-from-induced-pluripotent-stem-ips-cells
#18
Tamihiro Kawakami, Tatsuro Okano, Sora Takeuchi, Kayoko Osumi, Yoshinao Soma, Munenari Itoh, Tomohisa Hirobe, Kowichi Jimbow
Induced pluripotent stem (iPS) cells have the ability to differentiate into multiple cell types in the body and have an unlimited growth potential. However, iPS cell-derived melanocytes produced by existing protocols have significant limitations in developing novel strategies for regenerative medicine and cell therapies of pigmentation disorders in humans, because they involve culture in media containing fetal bovine serum (FBS) and non-physiologic agents. In this study, we established a new in vitro approach to generate iPS cell-derived human melanocytes which have higher proliferation rates and increased melanin production compared to melanocytes prepared by previously reported approaches...
September 5, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28885616/myd88-is-an-essential-component-of-retinoic-acid-induced-differentiation-in-human-pluripotent-embryonal-carcinoma-cells
#19
Gomaa Sulaiman, Aoife Cooke, Brendan Ffrench, Claudia Gasch, Olayemi Azeez Abdullai, Kevin O'Connor, Salah Elbaruni, Gordon Blackshields, Cathy Spillane, Helen Keegan, Victoria McEneaney, Ronan Knittel, Annamarie Rogers, Ian B Jeffery, Brendan Doyle, Mark Bates, Charles d'Adhemar, Mathia Yc Lee, Eric L Campbell, Paul N Moynagh, Desmond G Higgins, Sharon O'Toole, Luke O'Neill, John J O'Leary, Michael F Gallagher
We have previously reported that myeloid differentiation primary response gene 88 (MyD88) is downregulated during all-trans retinoic acid (RA)-induced differentiation of pluripotent NTera2 human embryonal carcinoma cells (hECCs), whereas its maintained expression is associated with RA differentiation resistance in nullipotent 2102Ep hECCs. MyD88 is the main adapter for toll-like receptor (TLR) signalling, where it determines the secretion of chemokines and cytokines in response to pathogens. In this study, we report that loss of MyD88 is essential for RA-facilitated differentiation of hECCs...
September 8, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28883470/cytoglobin-promotes-cardiac-progenitor-cell-survival-against-oxidative-stress-via-the-upregulation-of-the-nf%C3%AE%C2%BAb-inos-signal-pathway-and-nitric-oxide-production
#20
Shuning Zhang, Xiuchun Li, Frances L Jourd'heuil, Shunlin Qu, Neil Devejian, Edward Bennett, David Jourd'heuil, Chuanxi Cai
Human cardiac stem/progenitor cells (hCPCs) may serve in regenerative medicine to repair the infarcted heart. However, this approach is severely limited by the poor survival of donor cells. Recent studies suggest that the mammalian globin cytoglobin (CYGB) regulates nitric oxide (NO) metabolism and cell death. In the present study, we found that CYGB is expressed in hCPCs. Through molecular approaches aimed at increasing or decreasing CYGB expression in hCPCs, we found that CYGB functions as a pro-survival factor in response to oxidative stress...
September 7, 2017: Scientific Reports
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