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regenerative medicine with stem cells

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https://www.readbyqxmd.com/read/28646200/mesenchymal-stem-cells-secretome-induced-axonal-outgrowth-is-mediated-by-bdnf
#1
Luís F Martins, Rui O Costa, Joana R Pedro, Paulo Aguiar, Sofia C Serra, Fabio G Teixeira, Nuno Sousa, António J Salgado, Ramiro D Almeida
Mesenchymal stem cells (MSCs) have been used for cell-based therapies in regenerative medicine, with increasing importance in central and peripheral nervous system repair. However, MSCs grafting present disadvantages, such as, a high number of cells required for transplantation and low survival rate when transplanted into the central nervous system (CNS). In line with this, MSCs secretome which present on its composition a wide range of molecules (neurotrophins, cytokines) and microvesicles, can be a solution to surpass these problems...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28643176/a-promising-tool-in-retina-regeneration-current-perspectives-and-challenges-when-using-mesenchymal-progenitor-stem-cells-in-veterinary-and-human-ophthalmological-applications
#2
REVIEW
Anna Cislo-Pakuluk, Krzysztof Marycz
Visual impairment is a common ailment of the current world population, with more exposure to CCD screens and fluorescent lighting, approximately 285 billion people suffer from this deficiency and 13% of those are considered clinically blind. More common causes for visual impairment include age-related macular degeneration (AMD), glaucoma and diabetic retinopathy (Zhu et al. Molecular Medicine Reports, 2015; Kolb et al. 2007; Machalińska et al. Current Eye Research, 34(9),748-760, 2009) among a few. As cases of retinal and optic nerve diseases rise, it is vital to find a treatment, which has led to investigation of the therapeutic potential of various stem cells types (Bull et al...
June 22, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28641542/functional-nanoparticles-and-their-interactions-with-mesenchymal-stem-cells
#3
Weiwei Wang, Zijun Deng, Xun Xu, Zhengdong Li, Friedrich Jung, Nan Ma, Andreas Lendlein
Mesenchymal stem cells (MSCs) have become one of the most important cell sources for regenerative medicine. However, some mechanisms of MSC-based therapy are still not fully understood. The clinical outcome may be restricted by some MSC-related obstacles such as the low survival rate, differentiation into undesired lineages and malignant transformation. In recent years, with the emergence of nanotechnology, various types of multifunctional nanoparticles (NPs) have been designed, prepared and explored for bio-related applications...
June 22, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28638470/bright-polymer-dots-tracking-stem-cell-engraftment-and-migration-to-injured-mouse-liver
#4
Dandan Chen, Qiong Li, Zihui Meng, Lei Guo, Ying Tang, Zhihe Liu, Shengyan Yin, Weiping Qin, Zhen Yuan, Xuanjun Zhang, Changfeng Wu
Stem cell therapy holds promise for treatment of intractable diseases and injured organs. For clinical translation, it is pivotal to understand the homing, engraftment, and differentiation processes of stem cells in a living body. Here we report near-infrared (NIR) fluorescent semiconductor polymer dots (Pdots) for bright labeling and tracking of human mesenchymal stem cells (MSCs). The Pdots exhibit narrow-band emission at 775 nm with a quantum yield of 22%, among the highest value for various NIR probes. The Pdots together with a cell penetrating peptide are able to track stem cells over two weeks without disturbing their multipotent properties, as confirmed by the analyses on cell proliferation, differentiation, stem-cell markers, and immunophenotyping...
2017: Theranostics
https://www.readbyqxmd.com/read/28630143/extracellular-vesicles-of-stromal-origin-target-and-support-hematopoietic-stem-and-progenitor-cells
#5
Gregoire Stik, Simon Crequit, Laurence Petit, Jennifer Durant, Pierre Charbord, Thierry Jaffredo, Charles Durand
Extracellular vesicles (EVs) have been recently reported as crucial mediators in cell-to-cell communication in development and disease. In this study, we investigate whether mesenchymal stromal cells that constitute a supportive microenvironment for hematopoietic stem and progenitor cells (HSPCs) released EVs that could affect the gene expression and function of HSPCs. By taking advantage of two fetal liver-derived stromal lines with widely differing abilities to maintain HSPCs ex vivo, we demonstrate that stromal EVs play a critical role in the regulation of HSPCs...
June 19, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28626364/ex-vivo-generation-of-genetically-modified-macrophages-from-human-induced-pluripotent-stem-cells
#6
Mania Ackermann, Alexandra Kuhn, Jessica Kunkiel, Sylvia Merkert, Ulrich Martin, Thomas Moritz, Nico Lachmann
BACKGROUND: Pluripotent stem cells, including induced pluripotent stem cells (iPSCs), have the capacity to differentiate towards all three germ layers and have been highlighted as an attractive cell source for the field of regenerative medicine. Thus, stable expression of therapeutic transgenes in iPSCs, as well as thereof derived progeny of hematopoietic lineage, may lay the foundation for innovative cell replacement therapies. METHODS: We have utilized human iPSC lines genetically modified by lentiviral vector technology or targeted integration of reporter genes to evaluate transgene expression during hematopoietic specification and differentiation towards macrophages...
June 2017: Transfusion Medicine and Hemotherapy
https://www.readbyqxmd.com/read/28623616/cyclosporine-a-mediated-il-6-expression-promotes-neural-induction-in-pluripotent-stem-cells
#7
Ashwathnarayan Ashwini, Sushma S Naganur, Bhaskar Smitha, Preethi Sheshadri, Jyothi Prasanna, Anujith Kumar
Differentiation of pluripotent stem cells (PSCs) to neural lineages has gathered huge attention in both basic research and regenerative medicine. The major hurdle lies in the efficiency of differentiation and identification of small molecules that facilitate neurogenesis would partly circumvent this limitation. The small molecule Cyclosporine A (CsA), a commonly used immunosuppressive drug, has been shown to enhance in vivo neurogenesis. To extend the information to in vitro neurogenesis, we examined the effect of CsA on neural differentiation of PSCs...
June 16, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28621207/glycosaminoglycan-based-resorbable-polymer-composites-in-tissue-refurbishment
#8
Khushboo Gulati, Mukesh Kumar Meher, Krishna Mohan Poluri
Regeneration of tissue structure with the aid of bioactive polymer matrices/composites and scaffolds for respective applications is one of the emerging areas of biomedical engineering. Recent advances in conjugated glycosaminoglycan (GAG) hybrids using natural and synthetic polymers have opened new avenues for producing a wide variety of resorbable polymer matrices. These hybrid scaffolds are low-immunogenic, highly biocompatible and biodegradable with incredible mechanical and tensile properties. GAG-based resorbable polymeric matrices are being exploited in migration of stem cells, cartilage and bone replacement/regeneration and production of scaffolds for various tissue engineering applications...
June 16, 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/28621200/muscle-derived-stem-cells-in-peripheral-nerve-regeneration-reality-or-illusion
#9
Jing Zhou, Haiyan Cui, Haibin Lu, Zhuqiu Xu, Weifeng Feng, Lulu Chen, Xiaolei Jin, Xiaonan Yang, Zuoliang Qi
Owing to the complicated and time-consuming regenerative process, the repair of injured peripheral nerves depends largely on ongoing stem-cell therapy. Decades ago, researchers successfully isolated and identified muscle-derived stem cells (MDSCs) and discovered their potential for multidifferentiation. MDSCs play an important role in trauma repair associated with neuromuscular and vascular injury by simultaneously promoting tissue regrowth via direct differentiation and systematic secretion under physiological conditions...
June 16, 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/28621178/interview-with-bernard-siegel
#10
Bernard Siegel
Bernard Siegel is the Founder and full-time Executive Director of the nonprofit Regenerative Medicine Foundation (FL, USA). He is the Founder and the Co-chair of the World Stem Cell Summit series of global conferences and Editor-in-Chief of the World Stem Cell Report. He received his undergraduate and law degrees from the University of Miami (BA, 1972; JD, 1975) and has been a member of the Florida Bar since 1975. As a recognized policy expert relating to stem cell research, regenerative medicine and cloning, Bernard works with the world's leading stem cell researchers and advocates...
June 16, 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/28621167/interview-with-darrell-kotton
#11
Darrell Kotton
Dr Kotton is the David Seldin Professor of Medicine and the founding Director of the Center for Regenerative Medicine of Boston University and Boston Medical Center. He conducts basic stem cell research as an NIH-funded Principle Investigator in the Kotton Laboratory (focused on stem cell biology and gene therapy related to lung injury and repair). In addition, he attends in the Medical Intensive Care Unit, the Pulmonary Consultation Service, the Alpha-1 Center and the Pulmonary Outpatient Clinic, all at Boston Medical Center...
June 16, 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/28618138/expansion-of-umbilical-cord-blood-aldehyde-dehydrogenase-expressing-cells-generates-myeloid-progenitor-cells-that-stimulate-limb-revascularization
#12
David M Putman, Tyler T Cooper, Stephen E Sherman, Ayesh K Seneviratne, Mark Hewitt, Gillian I Bell, David A Hess
Uncompromised by chronic disease-related comorbidities, human umbilical cord blood (UCB) progenitor cells with high aldehyde dehydrogenase activity (ALDH(hi) cells) stimulate blood vessel regeneration after intra-muscular transplantation. However, implementation of cellular therapies using UCB ALDH(hi) cells for critical limb ischemia, the most severe form of severe peripheral artery disease, is limited by the rarity (<0.5%) of these cells. Our goal was to generate a clinically-translatable, allogeneic cell population for vessel regenerative therapies, via ex vivo expansion of UCB ALDH(hi) cells without loss of pro-angiogenic potency...
June 15, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28616376/genome-editing-and-muscle-stem-cells-as-a-therapeutic-tool-for-muscular-dystrophies
#13
REVIEW
Veronica Pini, Jennifer E Morgan, Francesco Muntoni, Helen C O'Neill
PURPOSE OF REVIEW: Muscular dystrophies are a group of severe degenerative disorders characterized by muscle fiber degeneration and death. Therapies designed to restore muscle homeostasis and to replace dying fibers are being experimented, but none of those in clinical trials are suitable to permanently address individual gene mutation. The purpose of this review is to discuss genome editing tools such as CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated), which enable direct sequence alteration and could potentially be adopted to correct the genetic defect leading to muscle impairment...
2017: Current Stem Cell Reports
https://www.readbyqxmd.com/read/28610596/complete-human-serum-maintains-viability-and-chondrogenic-potential-of-human-synovial-stem-cells-suitable-conditions-for-transplantation
#14
Mitsuru Mizuno, Hisako Katano, Koji Otabe, Keiichiro Komori, Yuji Kohno, Shizuka Fujii, Nobutake Ozeki, Masafumi Horie, Kunikazu Tsuji, Hideyuki Koga, Takeshi Muneta, Ichiro Sekiya
BACKGROUND: In our clinical practice, we perform transplantations of autologous synovial mesenchymal stem cells (MSCs) for cartilage and meniscus regenerative medicine. One of the most important issues to ensuring clinical efficacy involves the transport of synovial MSCs from the processing facility to the clinic. Complete human serum (100% human serum) is an attractive candidate material in which to suspend synovial MSCs for their preservation during transport. The purpose of this study was to investigate whether complete human serum maintained MSC viability and chondrogenic potential and to examine the optimal temperature conditions for the preservation of human synovial MSCs...
June 13, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28603415/electroactive-batio3-nanoparticle-functionalized-fibrous-scaffolds-enhance-osteogenic-differentiation-of-mesenchymal-stem-cells
#15
Yiping Li, Xiaohan Dai, Yunyang Bai, Yun Liu, Yuehong Wang, Ousheng Liu, Fei Yan, Zhangui Tang, Xuehui Zhang, Xuliang Deng
It has been proven that the surface topographic cues of fiber arrangement can induce osteogenic differentiation of mesenchymal stem cells. However, this effect alone is weak and insufficient to meet the needs of regenerative medicine. In this work, electroactivity concept was introduced to enhance the osteoinductivity of fibrous scaffolds. The randomly oriented and aligned electroactive fibrous scaffolds of poly-(l-lactic acid) (PLLA) with incorporation of ferroelectric ceramic BaTiO3 (BTO) nanoparticles (NPs) were fabricated by electrospinning...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28602136/in-situ-forming-hydrogels-with-long-lasting-mir-21-enhances-the-therapeutic-potential-of-msc-by-sustaining-stimulation-of-target-gene
#16
Xianwei Zhang, Yufa Sun, Jianfeng Liu, Zhiyong Yi, Feng Gao, Qiaowei Liu, Yundai Chen
Regulating stem cells by microRNA (miRNA) is promising in regenerative medicine. However, non-viral transfection is usually transient while stably lentiviral transfection is accompanied with oncogenic risk. In the study, we explored the feasibility to retain the microRNAs within biopolymer hydrogels for their long-lasting working and sustaining stimulation of target gene. miRNA-21 (MiR-21), a reported microRNA enhancing the therapeutic potential of mesenchymal stem cells (MSCs) was used. We demonstrated that miR-21 could be efficiently retained within collagen hydrogel after forming complex with cationic polymer polyethylenimine (PEI)...
June 10, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28596105/multifunctional-nanodiamonds-in-regenerative-medicine-recent-advances-and-future-directions
#17
REVIEW
Jonathan Whitlow, Settimio Pacelli, Arghya Paul
With recent advances in the field of nanomedicine, many new strategies have emerged for diagnosing and treating diseases. At the forefront of this multidisciplinary research, carbon nanomaterials have demonstrated unprecedented potential for a variety of regenerative medicine applications including novel drug delivery platforms that facilitate the localized and sustained release of therapeutics. Nanodiamonds (NDs) are a unique class of carbon nanoparticles that are gaining increasing attention for their biocompatibility, highly functional surfaces, optical properties, and robust physical properties...
June 5, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28595567/cartilage-tissue-regeneration-the-roles-of-cells-stimulating-factors-and-scaffolds
#18
Kui Huang, Qing Li, Yong Li, Zhihao Yao, Daowen Luo, Pengcheng Rao, Jingang Xiao
Cartilage tissue engineering is an emerging technique for the regeneration of cartilage tissue damaged as a result of trauma or disease. As the propensity for healing and regenerative capabilities of articular cartilage are limited, its repair remains one of the most challenging issues of musculoskeletal medicine. Clinical treatments intended to promote the success and complete repair of partial- and full-thickness articular cartilage defects are still unpredictable. However, one of the most exciting theories is that treatment of damaged articular cartilage can be realized with cartilage tissue engineering...
June 7, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28595566/a-systemic-review-of-the-sources-of-adult-mesenchymal-stem-cells-and-their-suitability-in-musculoskeletal-applications
#19
Rhiannon Nancarrow-Lei, Pouya Mafi, Reza Mafi, Wasim Khan
Background: Adult mesenchymal stem cells (MSCs) were first isolated from bone marrow by Friedenstein in 1976. These cells were clonogenic, non-haematopoietic, and able to replicate extensively in vitro. The fields of regenerative medicine and tissue engineering have grown dramatically since their inception. In the decades since, MSCs have been identified from mesoderm-, endoderm- and ectoderm-derived tissues. In light of our ageing population, the need for effective cell-based therapies for tissue repair and regeneration is ever-expanding...
June 8, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28594910/testosterone-improves-the-differentiation-efficiency-of-insulin-producing-cells-from-human-induced-pluripotent-stem-cells
#20
Haikun Liu, Dongsheng Guo, Aynisahan Ruzi, Yan Chen, Tingcai Pan, Fan Yang, Jialiang Li, Kecheng Xu, Tiancheng Zhou, Dajiang Qin, Yin-Xiong Li
Human induced pluripotent stem cells (hiPSCs) may provide potential resource for regenerative medicine research, including generation of insulin-producing cells for diabetes research and insulin production. Testosterone (T) is an androgen hormone which promotes protein synthesis and improves the management of type 2 diabetes in clinical studies. Concurrently, co-existed hyperandrogenism and hyperinsulinism is frequently observed in polycystic ovary syndrome, congenital adrenal hyperplasia and some of Wermer's syndrome...
2017: PloS One
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