Guochu Huang, Sheng Xie, Meng Wang, Dewen Mao, Guye Huang, Jingjing Huang, Xirong Liu, Rongzhen Zhang, Jiacheng Xie, Liang Jiang Huang, Chen Cheng, Fan Yao, Yu Zhong, Long Lin, Chun Yao
OBJECTIVE: To analyze the serum metabolite profiling of HBV-induced liver cirrhosis patients who have MHE, to seek out the specific biomarkers of MHE, to reveal the pathogenesis of MHE and to explore a promising approach for early diagnosis of MHE by using GC-TOFMS and UPLC-QTOFMS metabonomic analytical techniques in combination with bioinformatics and pattern recognition analysis methods. METHOD: Sera samples of 100 normal controls (NC group), 29 cases of HBV-induced liver cirrhosis patients who have MHE (MHE group) and 24 cases of HBV-induced liver cirrhosis patients without MHE (including 12 cases of compensated cirrhosis (CS group) and 12 cases of decompensated cirrhosis (DS group)) were collected and employed into GC-TOFMS and UPLC-QTOFMS platforms for sera metabolites detection, the outcome data were then analyzed by principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA)...
October 17, 2022: Biomedical Chromatography: BMC