keyword
MENU ▼
Read by QxMD icon Read
search

tracheal scaffold

keyword
https://www.readbyqxmd.com/read/29752586/comparison-of-tracheal-reconstruction-with-allograft-fresh-xenograft-and-artificial-trachea-scaffold-in-a-rabbit-model
#1
Jae Yeon Lee, Jeong Hun Park, Dong-Woo Cho
This study evaluated the possibility of tracheal reconstruction with allograft, pig-to-rabbit fresh xenograft or use of a tissue-engineered trachea, and compared acute rejection of three different transplanted tracheal segments in rabbits. Eighteen healthy New Zealand White rabbits weighing 2.5-3.1 kg were transplanted with three different types of trachea substitutes. Two rabbits and two alpha 1, 3-galactosyltransferase gene-knockout pigs weighing 5 kg were used as donors. The rabbits were divided into three groups: an allograft control group consisting of rabbit-to-rabbit allotransplantation animals (n = 6), a fresh xenograft group consisting of pig-to-rabbit xenotransplantation animals (n = 6), and an artificial trachea scaffold group (n = 6)...
May 12, 2018: Journal of Artificial Organs: the Official Journal of the Japanese Society for Artificial Organs
https://www.readbyqxmd.com/read/29658077/in-vivo-experimental-study-of-biological-compatibility-of-tissue-engineered-tracheal-construct-in-laboratory-primates
#2
I V Gilevich, A S Sotnichenko, D D Karal-Ogly, E A Gubareva, E V Kuevda, I S Polyakov, B A Lapin, S V Orlov, V A Porkhanov, V P Chekhonin
Biological compatibility of a tissue engineered construct of the trachea (synthetic scaffold) and allogenic mesenchymal stem cells was studied on laboratory Papio hamadryas primates. Subcutaneous implantation and orthotopic transplantations of tissue engineered constructs were carried out. Histological studies of the construct showed chaotically located filaments and mononuclear cells fixed to them. Development of a fine connective tissue capsule was found at the site of subcutaneous implantation of the tissue engineered construct...
April 16, 2018: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29469614/tgf-%C3%AE-1-induced-deposition-of-provisional-extracellular-matrix-by-tracheal-basal-cells-promotes-epithelial-to-mesenchymal-transition-in-a-jnk1-dependent-manner
#3
Jos L van der Velden, Darcy E Wagner, Karolyn G Lahue, Sarah T Abdalla, Ying W Lam, Daniel J Weiss, Yvonne M W Janssen-Heininger
Epithelial cells have been suggested as potential drivers of lung fibrosis, although the epithelial-dependent pathways that promote fibrogenesis remain unknown. Extracellular matrix is increasingly recognized as an environment that can drive cellular responses in various pulmonary diseases. In this study, we demonstrate that transforming growth factor- beta (TGF-β1)-stimulated mouse tracheal basal (MTB) cells produce provisional matrix proteins in vitro, which initiate mesenchymal changes in subsequently freshly plated MTB cells via Rho kinase- and c-Jun-N-terminal kinase (JNK1)-dependent processes...
February 22, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/29448837/restoring-tracheal-defects-in-a-rabbit-model-with-tissue-engineered-patches-based-on-tgf-%C3%AE-3-encapsulating-electrospun-poly-l-lactic-acid-co-%C3%AE%C2%B5-caprolactone-collagen-scaffolds
#4
Hui Jing, Botao Gao, Manchen Gao, Haiyue Yin, Xiumei Mo, Xiaoyang Zhang, Kai Luo, Bei Feng, Wei Fu, Jing Wang, Wei Zhang, Meng Yin, Zhongqun Zhu, Xiaomin He, Jinghao Zheng
Long segment tracheal stenosis often has a poor prognosis due to the limited availability of materials for tracheal reconstruction. Tissue engineered tracheal patches based on electrospun scaffolds and stem cells present ideal solutions to this medical challenge. However, the established engineering process is inefficient and time-consuming. In our research, to optimize the engineering process, core-shell nanofilms encapsulating TGF-β3 were fabricated as scaffolds for tracheal patches. The morphological and mechanical characteristics, degradation and biocompatibility of poly(l-lactic acid-co-ε-caprolactone)/collagen (PLCL/collagen) scaffolds with different compositions (PLCL:collagen 75:25, 50:50 and 25:75, respectively) were comparatively evaluated to determine the preferable compositional ratio...
February 15, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29346567/epithelial-grafting-of-a-decellularized-whole-tracheal-segment-an-in-vivo-experimental-model
#5
Margot Den Hondt, Bart M Vanaudenaerde, Eric K Verbeken, Jan J Vranckx
OBJECTIVES: Prerequisites for successful trachea transplantation include the use of a biocompatible construct, submucosal vascularization and an epithelial covering. Implantation of non-epithelialized tracheal scaffolds may lead to stenosis. However, epithelial grafting or seeding can only be attempted onto a well-vascularized submucosal bed. Our aim was to investigate a method to prevent stenosis during prelamination of non-epithelialized, gently decellularized rabbit tracheae and to evaluate whether grafting of revascularized constructs with buccal mucosa is feasible...
January 15, 2018: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29346562/scaffold-free-trachea-regeneration-by-tissue-engineering-with-bio-3d-printing
#6
Daisuke Taniguchi, Keitaro Matsumoto, Tomoshi Tsuchiya, Ryusuke Machino, Yosuke Takeoka, Abdelmotagaly Elgalad, Kiyofumi Gunge, Katsunori Takagi, Yasuaki Taura, Go Hatachi, Naoto Matsuo, Naoya Yamasaki, Koichi Nakayama, Takeshi Nagayasu
OBJECTIVES: Currently, most of the artificial airway organs still require scaffolds; however, such scaffolds exhibit several limitations. Alternatively, the use of an autologous artificial trachea without foreign materials and immunosuppressants may solve these issues and constitute a preferred tool. The rationale of this study was to develop a new scaffold-free approach for an artificial trachea using bio-3D printing technology. Here, we assessed the circumferential tracheal replacement using scaffold-free trachea-like grafts generated from isolated cells in an inbred animal model...
January 15, 2018: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29287858/designing-a-tissue-engineered-tracheal-scaffold-for-preclinical-evaluation
#7
Cameron A Best, Victoria K Pepper, Devan Ohst, Kyle Bodnyk, Eric Heuer, Ekene A Onwuka, Nakesha King, Robert Strouse, Jonathan Grischkan, Christopher K Breuer, Jed Johnson, Tendy Chiang
OBJECTIVE: Recent efforts to tissue engineer long-segment tracheal grafts have been complicated by stenosis and malacia. It has been proposed that both the mechanical characteristics and cell seeding capacity of TETG scaffolds are integral to graft performance. Our aim was to design a tracheal construct that approximates the biomechanical properties of native sheep trachea and optimizes seeding with bone marrow derived mononuclear cells prior to preclinical evaluation in an ovine model...
January 2018: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/29226358/mechanical-evaluation-of-tracheal-grafts-on-different-scales
#8
REVIEW
Fabio Gava Aoki, Henrique Takachi Moriya
Tissue engineered (or bioengineered) tracheas are alternative options under investigation when the resection with end-to-end anastomosis cannot be performed. One approach to develop bioengineered tracheas is a complex process that involves the use of decellularized tissue scaffolds, followed by recellularization in custom-made tracheal bioreactors. Tracheas withstand pressure variations and their biomechanics are of great importance so that they do not collapse during respiration, although there has been no preferred method of mechanical assay of tracheas among several laboratories over the years...
December 11, 2017: Artificial Organs
https://www.readbyqxmd.com/read/29226141/evaluation-of-immunosuppressive-therapy-use-for-tracheal-transplantation-with-trachea-mimetic-bellows-scaffolds-in-a-rabbit-model
#9
Jae Yeon Lee, Jeong Hun Park, Soo Jin Son, Mina Han, Gonhyung Kim, Seong Soo Kang, Seok Hwa Choi, Dong-Woo Cho
The objective of this study was to evaluate the use of immunosuppressive therapy with high-dose cyclosporine, high-dose azathioprine, and a combination of low-dose cyclosporine and azathioprine after tracheal reconstruction by using a trachea-mimetic graft of polycaprolactone (PCL) bellows-type scaffold in a rabbit model. Twenty-four healthy New Zealand white rabbits were used in the study. All underwent circumferential tracheal replacement using tissue-engineered tracheal graft, prepared from PCL bellows scaffold reinforced with silicone ring, collagen hydrogel, and human turbinate mesenchymal stromal cell (hTMSC) sheets...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29206488/structural-and-functional-evidence-for-the-scaffolding-effect-of-alveolar-blood-vessels
#10
Barry C Gibney, Willi L Wagner, Alexandra B Ysasi, Janeil M Belle, Akira Tsuda, Maximilian Ackermann, Steven J Mentzer
A contribution of pulmonary blood distension to alveolar opening was first proposed more than 100 years ago. To investigate the contribution of blood distension to lung mechanics, we studied control mice (normal perfusion), mice after exsanguination (absent perfusion) and mice after varying degrees of parenchymal resection (supra-normal perfusion). On inflation, mean tracheal pressures were higher in the bloodless mouse (4.0 ± 2.5 cm H2 O); however, there was minimal difference between conditions on deflation (0...
November 2017: Experimental Lung Research
https://www.readbyqxmd.com/read/29177132/autologous-cell-seeding-in-tracheal-tissue-engineering
#11
REVIEW
Elizabeth F Maughan, Robert E Hynds, Toby J Proctor, Sam M Janes, Martin Elliott, Martin A Birchall, Mark W Lowdell, Paolo De Coppi
Purpose of Review: There is no consensus on the best technology to be employed for tracheal replacement. One particularly promising approach is based upon tissue engineering and involves applying autologous cells to transplantable scaffolds. Here, we present the reported pre-clinical and clinical data exploring the various options for achieving such seeding. Recent Findings: Various cell combinations, delivery strategies, and outcome measures are described. Mesenchymal stem cells (MSCs) are the most widely employed cell type in tracheal bioengineering...
2017: Current Stem Cell Reports
https://www.readbyqxmd.com/read/28967085/tracheal-decellularization-using-a-combination-of-chemical-physical-and-bioreactor-methods
#12
Yourka D Tchoukalova, Justin M Hintze, Richard E Hayden, David G Lott
INTRODUCTION: The purpose of this study was to compare different decellularization protocols with the conventional detergent enzymatic method (DEM) using continuous agitation. METHODS: The first experiment compared conventional DEM with sonication and lyophilization+freeze-thaw cycles. A second experiment was carried out to compare time-adjusted DEM (2-hour instead of 4-hour incubations with 4% deoxycholate) to decellularization in a bioreactor. Cellularity was determined by DNA-quantitation, H&E-staining and immunostaining for major histocompatibility complex-1 (MHC-1)...
September 28, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28888019/in-vivo-characterization-of-a-3d-hybrid-scaffold-based-on-pcl-decellularized-aorta-for-tracheal-tissue-engineering
#13
Fariba Ghorbani, Lida Moradi, Mohammad Behgam Shadmehr, Shahin Bonakdar, Atosa Droodinia, Farzaneh Safshekan
INTRODUCTION: As common treatments for long tracheal stenosis are associated with several limitations, tracheal tissue engineering is considered as an alternative treatment. AIM OF STUDY: This study aimed at preparing a hybrid scaffold, based on biologic and synthetic materials for tracheal tissue engineering. MATERIALS AND METHODS: Three electrospun polycaprolactone (PCL) scaffolds, namely E1 (pure PCL), E2 (collagen-coated PCL) and E3 (PCL blended with collagen) were prepared...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28855683/tracheal-reconstruction-with-a-free-vascularized-myofascial-flap-preclinical-investigation-in-a-porcine-model-to-human-clinical-application
#14
Won Shik Kim, Jae Won Chang, Woo Soon Jang, Young Joon Seo, Mi-Lan Kang, Hak-Joon Sung, Da Hee Kim, Jung Min Kim, Jae Hong Park, Myung Jin Ban, Gina Na, Seung Ho Shin, Hyung Kwon Byeon, Yoon Woo Koh, Se-Heon Kim, Hong Koo Baik, Eun Chang Choi
Although there are various methods for tracheal reconstruction, such as a simple approximation with suturing and coverage with adjacent soft tissue or muscle, large defects >50% of the tracheal length still present a clinical challenge. Tissue engineering, a recent promising way to possibly resolve this problem, requires a long preparatory period for stem cell seeding on a scaffold and relatively invasive procedures for stem cell harvesting. As an alternative, we used a vascularized myofascial flap for tracheal reconstruction...
August 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28819688/airway-reconstruction-using-decellularized-tracheal-allografts-in-a-porcine-model
#15
Michinobu Ohno, Yasushi Fuchimoto, Huai-Che Hsu, Masataka Higuchi, Makoto Komura, Tetsuji Yamaoka, Akihiro Umezawa, Shin Enosawa, Tatsuo Kuroda
PURPOSE: Tracheal cartilage reconstruction is an essential approach for the treatment of tracheal congenital abnormalities or injury. Here, we evaluated the use of allogeneic decellularized tracheas as novel support scaffolds. METHODS: Six weaned pigs (4-week-old domestic males) were transplanted with allogeneic tracheal graft patches (three decellularized and three fresh tracheal scaffolds) onto artificial defects (approximately 15 × 15 mm). After 11 weeks, the tracheas were evaluated by bronchoscopy and histological studies...
October 2017: Pediatric Surgery International
https://www.readbyqxmd.com/read/28776693/a-comparison-of-tracheal-scaffold-strategies-for-pediatric-transplantation-in-a-rabbit-model
#16
COMPARATIVE STUDY
Elizabeth F Maughan, Colin R Butler, Claire Crowley, Gui Zhen Teoh, Margot den Hondt, Nicholas J Hamilton, Robert E Hynds, Peggy Lange, Tahera Ansari, Luca Urbani, Samuel M Janes, Paolo de Coppi, Martin A Birchall, Martin J Elliott
OBJECTIVES/HYPOTHESIS: Despite surgical advances, childhood tracheal stenosis is associated with high morbidity and mortality. Various tracheal scaffold strategies have been developed as the basis for bioengineered substitutes, but there is no consensus on which may be superior in vivo. We hypothesized that there would be no difference in morbidity and mortality between three competing scaffold strategies in rabbits. STUDY DESIGN: Pilot preclinical study. METHODS: Tracheal scaffolds were prepared by three methods that have been applied clinically and reported: preserved cadaveric ("Herberhold") allografts, detergent-enzymatically decellularized allografts, and synthetic scaffolds (nanocomposite polymer [polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane (POSS-PCU)])...
December 2017: Laryngoscope
https://www.readbyqxmd.com/read/28739545/an-optimized-non-destructive-protocol-for-testing-mechanical-properties-in-decellularized-rabbit-trachea
#17
M Den Hondt, B M Vanaudenaerde, E F Maughan, C R Butler, C Crowley, E K Verbeken, S E Verleden, J J Vranckx
Successful tissue-engineered tracheal transplantation relies on the use of non-immunogenic constructs, which can vascularize rapidly, support epithelial growth, and retain mechanical properties to that of native trachea. Current strategies to assess mechanical properties fail to evaluate the trachea to its physiological limits, and lead to irreversible destruction of the construct. Our aim was to develop and evaluate a novel non-destructive method for biomechanical testing of tracheae in a rabbit decellularization model...
September 15, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28730534/tracheal-cartilage-isolation-and-decellularization
#18
Zachary Galliger, Angela Panoskaltsis-Mortari
Tissue decellularization allows isolation of tissue extracellular matrix components, enabling bioengineering of native tissue microenvironments with minimal antigenic components. Here we describe a method to harvest decellularized cartilage tissue from donor trachea using a series of chemical and enzymatic washes and incorporating the extracellular matrix in gelatin methacrylate hydrogels. This decellularized cartilage tissue is easily incorporated into a variety of hydrogels to create a cartilage tissue scaffold...
July 21, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28719734/tissue-engineering-of-a-composite-trachea-construct-using-autologous-rabbit-chondrocytes
#19
James E Dennis, Kristina G Bernardi, Thomas J Kean, Nelson E Liou, Tanya K Meyer
The repair of large tracheal segmental defects remains an unsolved problem. The goal of this study is to apply tissue engineering principles for the fabrication of large segmental trachea replacements. Engineered tracheal replacements composed of autologous cells (neotracheas) were tested in a New Zealand White rabbit model. Neotracheas were formed in the rabbit neck by wrapping a silicone tube with consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet and allowing the construct to mature for 8-12 weeks...
March 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28701742/tissue-engineered-trachea-from-a-3d-printed-scaffold-enhances-whole-segment-tracheal-repair
#20
Manchen Gao, Hengyi Zhang, Wei Dong, Jie Bai, Botao Gao, Dekai Xia, Bei Feng, Maolin Chen, Xiaomin He, Meng Yin, Zhiwei Xu, Nevin Witman, Wei Fu, Jinghao Zheng
Long segmental repair of trachea stenosis is an intractable condition in the clinic. The reconstruction of an artificial substitute by tissue engineering is a promising approach to solve this unmet clinical need. 3D printing technology provides an infinite possibility for engineering a trachea. Here, we 3D printed a biodegradable reticular polycaprolactone (PCL) scaffold with similar morphology to the whole segment of rabbits' native trachea. The 3D-printed scaffold was suspended in culture with chondrocytes for 2 (Group I) or 4 (Group II) weeks, respectively...
July 12, 2017: Scientific Reports
keyword
keyword
8985
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"