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https://www.readbyqxmd.com/read/28934754/cntn-1-enhances-chemoresistance-in-human-lung-adenocarcinoma-through-induction-of-epithelial-mesenchymal-transition-by-targeting-the-pi3k-akt-pathway
#1
Ruijie Zhang, Shenghua Sun, Fuyun Ji, Chun Liu, Hua Lin, Lihua Xie, Honghui Yang, Wenxiang Tang, Yan Zhou, Jianping Xu, Pei Li
BACKGROUND/AIMS: Chemoresistance has been a major obstacle to the effective treatment of lung cancer. Previously, we found that contactin-1 (CNTN-1) is related to cisplatin resistance in lung adenocarcinoma. Here, we aimed to investigate the underlying mechanism behind the role of CNTN-1 in cisplatin resistance in lung adenocarcinoma. METHODS: EMT-associated phenotypes, including alterations in cellular morphology and marker (E-cadherin, N-cadherin and Vimentin) expression, were compared between A549 cells and A549/DDP cells (a cisplatin-resistant cell line of lung adenocarcinoma with abnormal CNTN-1 expression) by using real-time time PCR and Western blotting...
September 1, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28934726/application-of-adverse-outcome-pathways-to-u-s-epa-s-endocrine-disruptor-screening-program
#2
REVIEW
Patience Browne, Pamela D Noyes, Warren M Casey, David J Dix
BACKGROUND: The U.S. EPA's Endocrine Disruptor Screening Program (EDSP) screens and tests environmental chemicals for potential effects in estrogen, androgen, and thyroid hormone pathways, and it is one of the only regulatory programs designed around chemical mode of action. OBJECTIVES: This review describes the EDSP's use of adverse outcome pathway (AOP) and toxicity pathway frameworks to organize and integrate diverse biological data for evaluating the endocrine activity of chemicals...
September 1, 2017: Environmental Health Perspectives
https://www.readbyqxmd.com/read/28934681/simultaneous-determination-of-gentiopicroside-and-its-two-active-metabolites-in-rat-plasma-by-lc-ms-ms-and-its-application-in-pharmacokinetic-studies
#3
Kai Xiong, Tingting Gao, Tong Zhang, Zhengtao Wang, Han Han
Gentiopicroside is a natural secoiridoid glycoside that may require metabolic activation to exert pharmacological effects. In this study, two active metabolites of gentiopicroside (M1 and M2) were isolated from rat urines and identified with our previous method. Most importantly, a fast, sensitive and selective ultra high-performance liquid chromatography-tandem mass spectrometry method was developed to simultaneously determine gentiopicroside and its two metabolites in rat plasma. The analytes and internal standard (swertiamarin) were separated on an ACQUITY UPLC(®) BEH C18 column (2...
September 14, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28934662/a-pc-pu-nanoparticle-pu-decellularized-scaffold-composite-vascular-patch-synergistically-optimized-overall-performance-promotes-endothelialization
#4
Jun Zhang, Cheng Liu, Fuling Feng, Dawei Wang, Shuaishuai Lu, Guo Wei, Hong Mo, Tong Qiao
Composite vascular patches have gained increasingly attention due to the limited availability of autologous patches (vascular graft materials made from the blood vessels of the same recipient), the lack of growth capability of nonautologous patches (vascular graft materials made from the blood vessels of a different donor) and the disadvantages of synthetic patches. In this study, we report a highly biocompatible phosphatidylcholine-polyurethane nanoparticle/polyurethane/decellularized scaffold composite vascular patch (PCVP)...
September 6, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28934660/antimicrobial-activity-of-some-natural-extracts-encapsulated-within-silica-matrices
#5
Alexandra Duprates Steiner, Alvaro Vargas, Nei Fronza, Adriano Brandelli, João Henrique Zimnoch Dos Santos
Natural extracts (anthocyanins, tannin, anatto, curcuma and olive leaf extracts) were encapsulated within a silica network by acid or base-catalyzed sol-gel methods. The nominal encapsulated contents were between 2.5 and 50wt.-%. The resulting materials were characterized by Fourier transform infrared spectroscopy, diffuse reflectance in the UV region, nitrogen adsorption/desorption porosimetry and small angle X-ray scattering. Encapsulated anthocyanin and tannin afforded inhibition zones between 9-21mm towards Staphylococcus aureus, Escherichia coli, Bacillus cereus, Candida sp...
September 12, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28934502/blocking-the-reca-activity-and-sos-response-in-bacteria-with-a-short-%C3%AE-helical-peptide
#6
Alexander Yakimov, Georgii Pobegalov, Irina Bakhlanova, Mikhail Khodorkovskii, Michael Petukhov, Dmitry Baitin
The RecX protein, a very active natural RecA protein inhibitor, can completely disassemble RecA filaments at nanomolar concentrations that are two to three orders of magnitude lower than that of RecA protein. Based on the structure of RecX protein complex with the presynaptic RecA filament, we designed a short first in class α-helical peptide that both inhibits RecA protein activities in vitro and blocks the bacterial SOS-response in vivo. The peptide was designed using SEQOPT, a novel method for global sequence optimization of protein α-helices...
September 19, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28934494/derivatives-of-bst-like-gss-polymerase-with-improved-processivity-and-inhibitor-tolerance
#7
Igor P Oscorbin, Ekaterina A Belousova, Ulyana A Boyarskikh, Aleksandr I Zakabunin, Evgeny A Khrapov, Maksim L Filipenko
At the moment, one of the actual trends in medical diagnostics is a development of methods for practical applications such as point-of-care testing, POCT or research tools, for example, whole genome amplification, WGA. All the techniques are based on using of specific DNA polymerases having strand displacement activity, high synthetic processivity, fidelity and, most significantly, tolerance to contaminants, appearing from analysed biological samples or collected under purification procedures. Here, we have designed a set of fusion enzymes based on catalytic domain of DNA polymerase I from Geobacillus sp...
September 19, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28934419/characterizing-antigenic-determinants-in-helicobacter-pylori-caga-capable-of-detecting-serum-antibodies-in-children
#8
Min-Kyoung Shin, Jin-Su Jun, Soon-Wook Kwon, Dong-Hae Lee, Jong-Hun Ha, Jin-Sik Park, Hyung Lyun Kang, Seung Chul Baik, Ji Sook Park, Ji-Hyun Seo, Hee-Shang Youn, Myung Je Cho, Woo Kon Lee
Helicobacter pylori can persistently colonize the mucosa of the human stomach, resulting in gastric disorders. Endoscopic biopsy for rapid urease test and histopathologic examination are considered as the most accurate diagnostic methods for H. pylori infection. Serological methods are recommended for children because of invasiveness of the diagnosis mentioned above. Here, the cytotoxin-associated gene A protein (Cag A), as an immunodominant antigen, was subdivided to determine which regions harbor antigenicity for humans...
November 30, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28934372/antibacterial-effects-of-the-artificial-surface-of-nanoimprinted-moth-eye-film
#9
Kiyoshi Minoura, Miho Yamada, Takashi Mizoguchi, Toshihiro Kaneko, Kyoko Nishiyama, Mari Ozminskyj, Tetsuo Koshizuka, Ikuo Wada, Tatsuo Suzutani
The antibacterial effect of a nanostructured film, known as "moth-eye film," was investigated. The moth-eye film has artificially formed nano-pillars, consisting of hydrophilic resin with urethane acrylate and polyethylene glycol (PEG) derivatives, all over its surface that replicates a moth's eye. Experiments were performed to compare the moth-eye film with a flat-surfaced film produced from the same materials. The JIS Z2801 film-covering method revealed that the two films produced a decrease in Staphylococcus aureus and Esherichia coli titers of over 5 and 3 logs, respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28934340/using-rna-seq-data-to-select-reference-genes-for-normalizing-gene-expression-in-apple-roots
#10
Zhe Zhou, Peihua Cong, Yi Tian, Yanmin Zhu
Gene expression in apple roots in response to various stress conditions is a less-explored research subject. Reliable reference genes for normalizing quantitative gene expression data have not been carefully investigated. In this study, the suitability of a set of 15 apple genes were evaluated for their potential use as reliable reference genes. These genes were selected based on their low variance of gene expression in apple root tissues from a recent RNA-seq data set, and a few previously reported apple reference genes for other tissue types...
2017: PloS One
https://www.readbyqxmd.com/read/28934337/pyrene-conjugation-and-spectroscopic-analysis-of-hydroxypropyl-methylcellulose-compounds-successfully-demonstrated-a-local-dielectric-difference-associated-with-in-vivo-anti-prion-activity
#11
Kenta Teruya, Ayumi Oguma, Keiko Nishizawa, Hiroshi Kamitakahara, Katsumi Doh-Ura
Our previous study on prion-infected rodents revealed that hydroxypropyl methylcellulose compounds (HPMCs) with different molecular weights but similar composition and degree of substitution have different levels of long-lasting anti-prion activity. In this study, we searched these HPMCs for a parameter specifically associated with in vivo anti-prion activity by analyzing in vitro chemical properties and in vivo tissue distributions. Infrared spectroscopic and thermal analyses revealed no differences among HPMCs, whereas pyrene conjugation and spectroscopic analysis revealed that the fluorescence intensity ratio of peak III/peak I correlated with anti-prion activity...
2017: PloS One
https://www.readbyqxmd.com/read/28934286/denitrification-potential-of-the-eastern-oyster-microbiome-using-a-16s-rrna-gene-based-metabolic-inference-approach
#12
Ann Arfken, Bongkeun Song, Jeff S Bowman, Michael Piehler
The eastern oyster (Crassostrea virginica) is a foundation species providing significant ecosystem services. However, the roles of oyster microbiomes have not been integrated into any of the services, particularly nitrogen removal through denitrification. We investigated the composition and denitrification potential of oyster microbiomes with an approach that combined 16S rRNA gene analysis, metabolic inference, qPCR of the nitrous oxide reductase gene (nosZ), and N2 flux measurements. Microbiomes of the oyster digestive gland, the oyster shell, and sediments adjacent to the oyster reef were examined based on next generation sequencing (NGS) of 16S rRNA gene amplicons...
2017: PloS One
https://www.readbyqxmd.com/read/28934254/deep-reconstruction-model-for-dynamic-pet-images
#13
Jianan Cui, Xin Liu, Yile Wang, Huafeng Liu
Accurate and robust tomographic reconstruction from dynamic positron emission tomography (PET) acquired data is a difficult problem. Conventional methods, such as the maximum likelihood expectation maximization (MLEM) algorithm for reconstructing the activity distribution-based on individual frames, may lead to inaccurate results due to the checkerboard effect and limitation of photon counts. In this paper, we propose a stacked sparse auto-encoder based reconstruction framework for dynamic PET imaging. The dynamic reconstruction problem is formulated in a deep learning representation, where the encoding layers extract the prototype features, such as edges, so that, in the decoding layers, the reconstructed results are obtained through a combination of those features...
2017: PloS One
https://www.readbyqxmd.com/read/28934207/evaluation-of-the-pharmacokinetic-pharmacodynamic-relationship-of-praziquantel-in-the-schistosoma-mansoni-mouse-model
#14
Nada Abla, Jennifer Keiser, Mireille Vargas, Natalie Reimers, Helmut Haas, Thomas Spangenberg
After more than 40 years of use, Praziquantel (PZQ) still remains the drug of choice for the treatment of intestinal and urogenital schistosomiasis. Its anti-parasitic activity resides primarily in the (R)-enantiomer. Hitherto neither the molecular target nor the pharmacokinetic-pharmacodynamic relationship have been fully elucidated. Here we investigated the efficacy and pharmacokinetics of PZQ in the Schistosoma mansoni mouse model to determine the key factors that drive its efficacy. Dose-response studies with racemic PZQ with or without addition of an irreversible pan-cytochrome P450 (CYP) inhibitor, 1-aminobenzotriazole (ABT), were performed...
September 21, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28934127/removal-of-phenolic-compounds-from-water-using-sewage-sludge-based-activated-carbon-adsorption-a-review
#15
REVIEW
Nuhu Dalhat Mu'azu, Nabeel Jarrah, Mukarram Zubair, Omar Alagha
Due to their industrial relevance, phenolic compounds (PC) are amongst the most common organic pollutants found in many industrial wastewater effluents. The potential detrimental health and environmental impacts of PC necessitate their removal from wastewater to meet regulatory discharge standards to ensure meeting sustainable development goals. In recent decades, one of the promising, cost-effective and environmentally benign techniques for removal of PC from water streams has been adsorption onto sewage sludge (SS)-based activated carbon (SBAC)...
September 21, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28934101/cytotoxic-and-hypoglycemic-activity-of-triterpenoid-saponins-from-camellia-oleifera-abel-seed-pomace
#16
Tai-Mei Di, Shao-Lan Yang, Feng-Yu Du, Lei Zhao, Tao Xia, Xin-Fu Zhang
One new and three known triterpenoid saponins were isolated and identified from Camellia oleifera seeds through IR, NMR, HR-ESI-MS and GC-MS spectroscopic methods, namely oleiferasaponin A₃, oleiferasaponin A₁, camelliasaponin B₁, and camelliasaponin B₂. The structure of oleiferasaponin A₃ was elucidated as 16α-hydroxy-21β-O-angeloyl-22α-O-cinnamoyl-23α-aldehyde-28-dihydroxymethylene-olean-12-ene-3β-O-[β-d-galactopyranosyl-(1→2)]-[β-d-xylopyranosyl-(1→2)-β-d-galactopyranosyl-(1→3)]-β-d-gluco-pyranosiduronic acid...
September 21, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28934007/herbicidal-activity-of-slow-release-herbicide-formulations-in-wheat-stands-infested-by-weeds
#17
Natalia Zhila, Anastasiya Murueva, Anna Shershneva, Ekaterina Shishatskaya, Tatiana Volova
The present study reports the herbicidal activity of metribuzin and tribenuron-methyl embedded in the degradable matrix of natural poly-3-hydroxybutyrate [P(3HB)/MET and P(3HB)/TBM]. The developed formulations were constructed as films and microgranules, which were tested against the weeds such as white sweet clover Melilotus albus and lamb's quarters Chenopodium album in the presence of soft spring wheat (Triticum aestivum, cv. Altaiskaya 70) as the subject crop for investigation. The activity was measured in laboratory scale experiments by determining the density and weight of the vegetative organs of weeds...
September 21, 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/28933871/neuropsychology-and-cognitive-neuroscience-in-the-fmri-era-a-recapitulation-of-localizationist-and-connectionist-views
#18
Matthew J Sutterer, Daniel Tranel
OBJECTIVE: We highlight the past 25 years of cognitive neuroscience and neuropsychology, focusing on the impact to the field of the introduction in 1992 of functional MRI (fMRI). METHOD: We reviewed the past 25 years of literature in cognitive neuroscience and neuropsychology, focusing on the relation and interplay of fMRI studies and studies utilizing the "lesion method" in human participants with focal brain damage. RESULTS: Our review highlights the state of localist/connectionist research debates in cognitive neuroscience and neuropsychology circa 1992, and details how the introduction of fMRI into the field at that time catalyzed a new wave of efforts to map complex human behavior to specific brain regions...
September 21, 2017: Neuropsychology
https://www.readbyqxmd.com/read/28933825/the-surface-chemistry-of-perovskite-type-electrodes-during-high-temperature-co2-electrolysis-investigated-by-operando-photoelectron-spectroscopy
#19
Alexander Karl Opitz, Andreas Nenning, Christoph Rameshan, Markus Kubicek, Thomas Götsch, Raoul Blume, Michael Hävecker, Axel Knop-Gericke, Gunther Rupprechter, Bernhard Klötzer, Juergen Fleig
Any substantial move of energy sources from fossil fuels to renewable resources requires large scale storage of excess energy, for example via power to fuel processes. In this respect electrochemical reduction of CO2 may become very important, since it offers a method of sustainable CO production, which is a crucial prerequisite for synthesis of sustainable fuels. Carbon dioxide reduction in solid oxide electrolysis cells (SOECs) is particularly promising owing to the high operating temperature, which leads to both improved thermodynamics and fast kinetics...
September 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28933816/reborn-from-the-ashes-turning-organic-molecules-to-antimicrobial-carbon-quantum-dots
#20
Scott G Harroun, Jui-Yang Lai, Chih-Ching Huang, Shou-Kuan Tsai, Han-Jia Lin
Using polyamines as the initial organic raw material and by applying simple pyrolysis methods, super cationic carbon quantum dots (CQDs) can easily be made. Since polyamines are natural products and the synthesis procedure is green, these polyamine-derived CQDs display low toxicity and high biocompatibility but possess high antibacterial activity. In addition, polyamine-derived CQDs display other unique properties, such as facilitation of wound healing and passage through the tight junction, which make them a very promising bactericide in future clinical applications...
September 21, 2017: ACS Infectious Diseases
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