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Aeromonas hydrophila

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https://www.readbyqxmd.com/read/28330807/dietary-myrtle-myrtus-communis-l-improved-non-specific-immune-parameters-and-bactericidal-activity-of-skin-mucus-in-rainbow-trout-oncorhynchus-mykiss-fingerlings
#1
Hadis Mansouri Taei, Abdolmajid Hajimoradloo, Seyed Hossein Hoseinifar, Hassan Ahmadvand
The present study examined the effects of dietary Myrtle (Myrtus communis L.) on non-specific immune parameters and bactericidal activity of skin mucus in rainbow trout (Oncorhynchus mykiss) fingerlings. Three hundred and sixty fingerlings (6.50 ± 0.55 g (were distributed in twelve cages (65 × 65 × 65 cm) with a metal framework. The study included four treatments repeated in triplicates. The treatments were feeding trouts with experimental diets containing different levels (0, 0.5, 1 and 1.5%) of Myrtle powder...
March 18, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28302886/mycoplasma-bovis-arthritis-and-pneumonia-in-calves
#2
(no author information available yet)
Mycoplasma bovis arthritis and pneumonia in six-month-old calvesSudden deaths in housed suckler cows due to hypomagnesaemiaBovine respiratory syncytial virus infection in two-year-old heifersBovine abortion associated with Parachlamydia speciesFibrinous pericarditis due to Aeromonas hydrophila in weaner pigsThese are among matters discussed in the disease surveillance report for December 2016 from SAC Consulting: Veterinary Services (SAC C VS).
March 18, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28302770/genome-sequence-of-hypervirulent-aeromonas-hydrophila-strain-hzauah
#3
Lin Teng, Limei Deng, Xingxing Dong, Shun Wei, Jinquan Li, Ningqiu Li, Yang Zhou
Aeromonas hydrophila, a zoonotic bacterium found in an expansive range of aquatic ecosystems, has been reported to cause severe diseases in fish, amphibians, reptiles, and mammals, including humans. Herein, we report the draft genome of the hypervirulent A. hydrophila strain HZAUAH isolated from a crucian in China.
March 16, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28300581/a-galectin-contributes-to-the-innate-immune-recognition-and-elimination-of-pathogens-in-the-freshwater-mussel-hyriopsis-cumingii
#4
Zhiyi Bai, Liting Zhao, Xiajun Chen, Qingqing Li, Jiale Li
Galectins are members of the lectin superfamily. They function as pattern recognition receptors in the innate immune system of vertebrates and invertebrates. A galectin homolog from the triangle sail mussel Hyriopsis cumingii (HcGal2) was cloned and characterized. HcGal2 mRNA was expressed in all tissues examined, displaying particular enrichment in mantle tissue. Interestingly, rHcGAL2 protein was only detected in the mantle, hemocytes, and gills, suggesting that post-transcriptional regulation may occur. HcGal2 expression was induced in the mantle, liver, and hemocytes after exposure to lipopolysaccharides, Gram-negative bacteria (Aeromonas hydrophila), and Gram-positive bacteria (Staphylococcus aureus)...
March 11, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28299401/sodium-lauryl-ether-sulfate-sles-degradation-by-nitrate-reducing-bacteria
#5
Ana M S Paulo, Rozelin Aydin, Mauricio R Dimitrov, Harm Vreeling, Ana J Cavaleiro, Pedro A García-Encina, Alfons J M Stams, Caroline M Plugge
The surfactant sodium lauryl ether sulfate (SLES) is widely used in the composition of detergents and frequently ends up in wastewater treatment plants (WWTPs). While aerobic SLES degradation is well studied, little is known about the fate of this compound in anoxic environments, such as denitrification tanks of WWTPs, nor about the bacteria involved in the anoxic biodegradation. Here, we used SLES as sole carbon and energy source, at concentrations ranging from 50 to 1000 mg L(-1), to enrich and isolate nitrate-reducing bacteria from activated sludge of a WWTP with the anaerobic-anoxic-oxic (A(2)/O) concept...
March 15, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28283525/molecular-viability-testing-of-uv-inactivated-bacteria
#6
Kris M Weigel, Felicia K Nguyen, Moira R Kearney, John S Meschke, Gerard A Cangelosi
The polymerase chain reaction (PCR) is effective at detecting bacterial DNA in samples, but it is unable to differentiate viable bacteria from inactivated cells or free DNA fragments. New PCR-based analytical strategies have been developed to address this limitation. Molecular viability testing (MVT) correlates bacterial viability with the ability to rapidly synthesize species-specific ribosomal RNA precursor (pre-rRNA) in response to brief nutritional stimulation. Previous studies demonstrated that MVT can assess bacterial inactivation by chlorine, serum, and low-temperature pasteurization...
March 10, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28279793/identification-and-characterization-of-a-novel-conserved-46-kd-maltoporin-of-aeromonas-hydrophila-as-a-versatile-vaccine-candidate-in-european-eel-anguilla-anguilla
#7
Jianjun Feng, Peng Lin, Songlin Guo, Yuanyuan Jia, Yilei Wang, Frank Zadlock, Ziping Zhang
European eel (Anguilla anguilla) is a crucial economic fish that has been plagued by Aeromonas hydrophila infections for many years. Vaccines that are cross-protective against multiple serotypes could provide an effective control against A. hydrophila-mediated diseases. The outer membrane proteins (OMPs) are highly immunogenic and capable of eliciting protective immune responses. This study reports the identification of a novel 46 kD maltoporin that is a conserved protective antigen for different serotypes of A...
March 6, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28279791/methionine-hydroxy-analogue-improves-intestinal-immunological-and-physical-barrier-function-in-young-grass-carp-ctenopharyngodon-idella
#8
Fei-Yu Pan, Pei Wu, Lin Feng, Wei-Dan Jiang, Sheng-Yao Kuang, Ling Tang, Wu-Neng Tang, Yong-An Zhang, Xiao-Qiu Zhou, Yang Liu
This study was conducted to test the hypothesis that methionine hydroxy analogue (MHA) enhances the defense against enteritis occurrence via improving intestinal barrier function in fish. After 630 young grass carp (Ctenopharyngodon idella) (259.70 ± 0.47 g) fed six graded levels of MHA (0, 2.4, 4.4, 6.4, 8.5 and 10.5 g/kg diet) and one dl-methionine group (6.4 g/kg diet) for 8 weeks. At the end of feeding trial, 15 fish from each treatment were challenged with Aeromonas hydrophila for 14 days. The results indicated that optimal MHA enhanced the capacity of fish against enteritis emergence, which might be related to the positive effects of MHA on intestinal immunological and physical barrier function in fish...
March 6, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28271383/global-protein-expression-profile-response-of-planktonic-aeromonas-hydrophila-exposed-to-chlortetracycline
#9
Wanxin Li, Zujie Yao, Xiangyu Zhang, Fang Huang, Wenxiong Lin, Xiangmin Lin
The antibiotics resistance phenomena of Aeromonas hydrophila has become serious economic and public health problems for the world aquaculture industry and human health care. In this study, to investigate the instinct antibiotics adaptive mechanism of this pathogen, iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) based quantitative proteomics technologies were performed to compare the differential expression of A. hydrophila in planktonic status in response to chlortetracycline (CTC) stress and then identified total 1552 proteins including 285 altered proteins with 90 increasing and 195 decreasing abundance proteins...
April 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28264491/comparative-genomics-of-the-aeromonadaceae-core-oligosaccharide-biosynthetic-regions
#10
Gabriel Forn-Cuní, Susana Merino, Juan M Tomás
Lipopolysaccharides (LPSs) are an integral part of the Gram-negative outer membrane, playing important organizational and structural roles and taking part in the bacterial infection process. In Aeromonas hydrophila, piscicola, and salmonicida, three different genomic regions taking part in the LPS core oligosaccharide (Core-OS) assembly have been identified, although the characterization of these clusters in most aeromonad species is still lacking. Here, we analyse the conservation of these LPS biosynthesis gene clusters in the all the 170 currently public Aeromonas genomes, including 30 different species, and characterise the structure of a putative common inner Core-OS in the Aeromonadaceae family...
February 28, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28259778/molecular-characterization-and-expression-analysis-of-toll-like-receptors-5-and-22-from-natural-triploid-carassius-auratus
#11
Jie Zhang, Li Wang, Yanjing Zhao, Xianghui Kong, Fan Wu, Xianliang Zhao
Innate immunity, as the most primitive and universal host defense in fish, constitutes an efficient first line of defense to combat invading microbes. Toll-like receptors (TLRs) play essential roles in the innate immunity, and TLR5 and TLR22 are two important TLRs that can recognize flagellin and double stranded RNA (dsRNA), respectively. In this study, we identified and characterized two TLRs genes of Qihe crucian carp (Carassius auratus) (designated as CaTLR5 and CaTLR22). The full-length cDNA sequence of CaTLR5 was cloned with 2972 bp including a 140 bp 5'-terminal untranslated region (UTR), a 183 bp 3'-UTR, and a 2649 bp open reading frame (ORF) encoding a deduced protein with 882 amino acids...
March 2, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28246446/incidence-and-virulence-characteristics-of-aeromonas-spp-in-fish
#12
Ashraf M Abd-El-Malek
AIM: This study was conducted to evaluate the presence of Aeromonas spp. in raw and ready-to-eat (RTE) fish commonly consumed in Assiut city, Egypt, and to determine virulence factors due to they play a key role in their pathogenicity. MATERIALS AND METHODS: A total of 125 samples of raw and RTE fish samples were taken from different fish markets and fish restaurants in Assiut Governorate and screened for the presence of Aeromonas spp. by enrichment on tryptic soy broth then incubated at 30°C for 24 h...
January 2017: Veterinary World
https://www.readbyqxmd.com/read/28238880/analysis-on-the-expression-and-function-of-a-chicken-type-and-goose-type-lysozymes-in-chinese-giant-salamanders-andrias-davidianus
#13
Hui Yang, Ranran Liu, Dan Cui, Haixia Liu, Dongmei Xiong, Xiaolin Liu, Lixin Wang
Lysozymes as an important immune factor, play vital roles in innate immune response against pathogen infection. In the present study, one c-type and g-type lysozymes were identified from Chinese giant salamander (Andrias davidianus). They shared highly conserved structural features with lysozymes from other species. Spatial expression analysis revealed that AdlysC transcript was most abundant in liver and stomach, and least in muscle and brain. In contrast, the expression level of AdlysG was most abundant in liver and least in muscle and skin...
February 24, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28236008/molecular-cloning-and-expression-analysis-of-megalobrama-amblycephala-transferrin-gene-and-effects-of-exposure-to-iron-and-infection-with-aeromonas-hydrophila
#14
Tao Teng, Bingwen Xi, Jun Xie, Kai Chen, Pao Xu, Liangkun Pan
Transferrin (Tf) plays an important function in iron homeostasis and metabolism of organisms. In this study, we identified and characterized the Tf gene in Megalobrama amblycephala and evaluated its expression in basal conditions as well as after iron overload and experimental infection with Aeromonas hydrophila. Furthermore, we studied the iron binding properties of recombinant Tf. The full-length M. amblycephala Tf complementary DNA (cDNA) (GenBank accession no.: KX698308) of 2245 bp was cloned and contained a 1953 bp open reading frame (ORF) encoding 650 amino acid residues and flanked by a 68 bp 5' and a 204 bp 3' untranslated regions (UTR)...
February 24, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28223111/expression-analysis-of-nine-toll-like-receptors-in-yellow-catfish-pelteobagrus-fulvidraco-responding-to-aeromonas-hydrophila-challenge
#15
Xiao-Ting Zhang, Gui-Rong Zhang, Ze-Chao Shi, Yu-Jie Yuan, Huan Zheng, Li Lin, Kai-Jian Wei, Wei Ji
Toll-like receptors (TLRs) are important components of pattern recognition receptors (PRRs), which play significant roles in innate immunity to defense against pathogen invasion. Many TLRs have been found in teleosts, but there are no reports about cloning and expression of TLR genes in yellow catfish (Pelteobagrus fulvidraco). In this study, we analyzed the sequence characters and the relative mRNA expression levels of nine TLRs (TLR1, TLR2, TLR3, TLR4-1, TLR5, TLR7, TLR8-2, TLR9 and TLR22) in different tissues of yellow catfish...
February 20, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28223110/h2a-and-ca-l-hipposin-gene-characteristic-analysis-and-expression-responses-to-aeromonas-hydrophila-infection-in-carassius-aurutus
#16
Xianghui Kong, Xiangmin Wu, Chao Pei, Jie Zhang, Xianliang Zhao, Li Li, Guoxing Nie, Xuejun Li
Antimicrobial peptide is an important component of the host innate immune system and thus serves a crucial function in host defense against microbial invasion. In this study, H2A and derived antimicrobial peptide Ca-L-hipposin were cloned and characterized in Carassius aurutus. The gene H2A full-length cDNA is 908 bp and includes a 5'-terminal untranslated region (UTR) of 55 bp and a 3'-terminal UTR of 466 bp with a canonical polyadenylation signal sequence AATAA, as well as an open reading frame (ORF) of 387 bp encoding a polypeptide of 128 amino acids, with a molecular weight of 13...
February 20, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28215743/a-nlrc3-like-gene-from-blunt-snout-bream-megalobrama-amblycephala-molecular-characterization-expression-and-association-with-resistance-to-aeromonas-hydrophila-infection
#17
Fengjuan Zhou, Qifeng Zhan, Zhujin Ding, Lina Su, Jun Fan, Lei Cui, Nan Chen, Weimin Wang, Hong Liu
NLRC (the nucleotide-oligomerization domain (NOD)-like receptor subfamily C) consists of teleost-specific NLRs (NOD-like receptors) and plays pivotal roles in microbial recognition and regulation of innate immune response. In this study, we cloned and characterized a NLRC3-like gene (MamNLRC3-like) from blunt snout bream (Megalobrama amblycephala) by using the quantitative real-time PCR method, and analyzed the correlation between its polymorphisms and resistance to Aeromonas hydrophila infection. The full length cDNA of MamNLRC3-like was 2863 bp, with a 5'-UTR of 169 bp, ORF of 2301 bp and 3'-UTR of 393 bp, encoding 766 amino acid residues...
February 20, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28209550/pathogenicity-of-mexican-isolates-of-aeromonas-sp-in-immersion-experimentally-infected-rainbow-trout-oncorhynchus-mykiss-walbaum-1792
#18
Andrea Paloma Zepeda-Velázquez, Vicente Vega-Sánchez, César Ortega-Santana, Miguel Rubio-Godoy, Dany Montes de Oca-Mira, Edgardo Soriano-Vargas
Ten species of Aeromonas have been previously identified in farmed rainbow trout from Mexico. The aim of the current study was to investigate the pathogenicity of 10 Aeromonas isolates belonging to 10 different Aeromonas species in immersion experimentally-infected rainbow trout fry. Isolates of A. bestiarum, A. hydrophila, A. salmonicida, and A. veronii produced significant mortality (8.8%, 12.2%, 18.8%, and 8.8%, respectively). Isolates of A. caviae and A. sobria produced no significantly mortality (3.3% and 1...
May 2017: Acta Tropica
https://www.readbyqxmd.com/read/28205238/comparative-pharmacokinetics-of-enrofloxacin-in-healthy-and-aeromonas-hydrophila-infected-crucian-carp-carassius-auratus-gibelio
#19
J Fan, Q Shan, J Wang, S Liu, L Li, G Zheng
The comparative pharmacokinetics of enrofloxacin (ENR) and its metabolite ciprofloxacin (CIP) were investigated in healthy and Aeromonas hydrophila-infected crucian carp after a single oral (p.o.) administration at a dose of 10 mg/kg at 25 °C. The plasma concentrations of ENR and of CIP were determined by HPLC. Pharmacokinetic parameters were calculated based on mean ENR concentrations by noncompartmental modeling. In healthy fish, the elimination half-life (T1/2λz ), maximum plasma concentration (Cmax ), time to peak (Tmax ), and area under the concentration-time curve (AUC) values were 64...
February 15, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28138102/alterations-in-peptidoglycan-cross-linking-suppress-the-secretin-assembly-defect-caused-by-mutation-of-gspa-in-the-type-ii-secretion-system
#20
Elizabeth M Vanderlinde, Timothy G Strozen, Sara B Hernández, Felipe Cava, S Peter Howard
In Gram-negative bacteria, the peptidoglycan (PG) cell wall is a significant structural barrier for outer membrane protein assembly. In Aeromonas hydrophila, outer membrane multimerization of the type II secretion system (T2SS) secretin ExeD requires the function of the inner membrane assembly factor complex ExeAB. The putative mechanism of the complex involves the reorganization of PG and localization of ExeD, whereby ExeA functions by interacting with PG to form a site for secretin assembly and ExeB forms an interaction with ExeD...
January 30, 2017: Journal of Bacteriology
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