keyword
MENU ▼
Read by QxMD icon Read
search

Spind

keyword
https://www.readbyqxmd.com/read/29685992/spindly-is-required-for-rapid-migration-of-human-cells
#1
Claudia Conte, Michelle A Baird, Michael W Davidson, Eric R Griffis
Dynein is the sole processive minus-end directed microtubule motor found in animals. It has roles in cell division, membrane trafficking, and cell migration. Together with dynactin, dynein regulates centrosomal orientation to establish and maintain cell polarity, controls focal adhesion turnover and anchors microtubules at the leading edge. In higher eukaryotes, dynein/dynactin requires additional components such as Bicaudal D to form an active motor complex and for regulating its cellular localization. Spindly is a protein that targets dynein/dynactin to kinetochores in mitosis and can activate its motility in vitro However, no role for Spindly in interphase dynein/dynactin function has been found...
April 23, 2018: Biology Open
https://www.readbyqxmd.com/read/29674933/spind-an-r-package-to-account-for-spatial-autocorrelation-in-the-analysis-of-lattice-data
#2
Gudrun Carl, Sam C Levin, Ingolf Kühn
spind is an R package aiming to provide a useful toolkit to account for spatial dependence in the analysis of lattice data. Grid-based data sets in spatial modelling often exhibit spatial dependence, i.e. values sampled at nearby locations are more similar than those sampled further apart. spind methods, described here, take this kind of two-dimensional dependence into account and are sensitive to its variation across different spatial scales. Methods presented to account for spatial autocorrelation are based on the two fundamentally different approaches of generalised estimating equations as well as wavelet-revised methods...
2018: Biodiversity Data Journal
https://www.readbyqxmd.com/read/29615558/requirement-of-the-dynein-adaptor-spindly-for-mitotic-and-post-mitotic-functions-in-drosophila
#3
Giuliana D Clemente, Matthew R Hannaford, Hamze Beati, Katja Kapp, Jens Januschke, Eric R Griffis, Hans-Arno J Müller
Spindly was originally identified as a specific regulator of Dynein activity at the kinetochore. In early prometaphase, Spindly recruits the Dynein/Dynactin complex, promoting the establishment of stable kinetochore-microtubule interactions and progression into anaphase. While details of Spindly function in mitosis have been worked out in cultured human cells and in the C. elegans zygote, the function of Spindly within the context of an organism has not yet been addressed. Here, we present loss- and gain-of-function studies of Spindly using transgenic RNAi in Drosophila ...
March 30, 2018: Journal of Developmental Biology
https://www.readbyqxmd.com/read/29515126/a-conserved-interaction-of-the-dynein-light-intermediate-chain-with-dynein-dynactin-effectors-necessary-for-processivity
#4
In-Gyun Lee, Mara A Olenick, Malgorzata Boczkowska, Clara Franzini-Armstrong, Erika L F Holzbaur, Roberto Dominguez
Cytoplasmic dynein is the major minus-end-directed microtubule-based motor in cells. Dynein processivity and cargo selectivity depend on cargo-specific effectors that, while generally unrelated, share the ability to interact with dynein and dynactin to form processive dynein-dynactin-effector complexes. How this is achieved is poorly understood. Here, we identify a conserved region of the dynein Light Intermediate Chain 1 (LIC1) that mediates interactions with unrelated dynein-dynactin effectors. Quantitative binding studies map these interactions to a conserved helix within LIC1 and to N-terminal fragments of Hook1, Hook3, BICD2, and Spindly...
March 7, 2018: Nature Communications
https://www.readbyqxmd.com/read/29478697/the-fluid-dynamics-of-balanus-glandula-barnacles-adaptations-to-sheltered-and-exposed-habitats
#5
Maureen Vo, Sasan Mehrabian, Fernando Villalpando, Stephane Etienne, Dominique Pelletier, Christopher B Cameron
Suspension feeders use a wide range of appendages to capture particles from the surrounding fluid. Their functioning, either as a paddle or a sieve, depends on the leakiness, or amount of fluid that passes through the gaps between the appendages. Balanus glandula is the most common species of barnacle distributed along the Pacific coast of North America. It shows a strong phenotypic response to water flow velocity. Individuals from exposed, high flow sites have short and robust cirral filters, whereas those from sheltered, low velocity sites have long, spindly appendages...
April 11, 2018: Journal of Biomechanics
https://www.readbyqxmd.com/read/29475948/antagonism-between-the-dynein-and-ndc80-complexes-at-kinetochores-controls-the-stability-of-kinetochore-microtubule-attachments-during-mitosis
#6
Mohammed A Amin, Richard J McKenney, Dileep Varma
Chromosome alignment and segregation during mitosis require kinetochore-microtubule (kMT) attachments that are mediated by the molecular motor dynein and the kMT-binding complex Ndc80. The Rod-ZW10-Zwilch (RZZ) complex is central to this coordination as it has an important role in dynein recruitment and has recently been reported to have a key function in the regulation of stable kMT attachments in Caenorhabditis elegans besides its role in activating the spindle assembly checkpoint (SAC). However, the mechanism by which these protein complexes control kMT attachments to drive chromosome motility during early mitosis is still unclear...
April 20, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29463901/poly-adp-ribose-mediates-asymmetric-division-of-mouse-oocyte
#7
Bingteng Xie, Lu Zhang, Huiling Zhao, Qingyun Bai, Yong Fan, Xiaohui Zhu, Yang Yu, Rong Li, Xin Liang, Qing-Yuan Sun, Mo Li, Jie Qiao
Before fertilization, mammalian oocyte undergoes an asymmetric division which depends on eccentric positioning of the spindle at the oocyte cortex to form a polar body and an egg. Since the centriole is absent and, as a result, the polar array microtubules are not fully developed in oocytes, microtubules have seldom been considered as required for eccentric positioning of the spindle, while actin-related forces have instead been proposed to be primarily responsible for this process. However, the existing models are largely conflicting and the underlying mechanism of asymmetric division is still elusive...
April 2018: Cell Research
https://www.readbyqxmd.com/read/29280338/rodlike-supramolecular-nanoassemblies-of-degradable-poly-aspartic-acid-derivatives-and-hydroxyl-rich-polycations-for-effective-delivery-of-versatile-tumor-suppressive-ncrnas
#8
Hai-Qing Song, Wenting Pan, Rui-Quan Li, Bingran Yu, Wenjuan Liu, Ming Yang, Fu-Jian Xu
The delivery of tumor-suppressive noncoding RNAs (ncRNAs) including short ncRNAs (i.e., miRNAs) and long ncRNAs (lncRNAs) is put forward to treat tumors. In this work, novel rodlike supramolecular nanoassemblies (CNC @CB[8] @ PGEA) of degradable poly(aspartic acid) (PAsp) derivatives-grafted cellulose nanocrystals (CNCs) and hydroxyl-rich polycations (ethanolamine-functionalized poly(glycidyl methacrylate), PGEA) are proposed via typical cucurbit[8]uril (CB[8])-based host-guest interactions for delivery of different ncRNAs to treat hepatocellular carcinoma (HCC)...
March 2018: Small
https://www.readbyqxmd.com/read/29192061/nude-regulates-dynein-at-kinetochores-but-is-dispensable-for-other-dynein-functions-in-the-c-elegans-early-embryo
#9
Patrícia A Simões, Ricardo Celestino, Ana X Carvalho, Reto Gassmann
In mitosis, the molecular motor dynein is recruited to kinetochores by the Rod-Zw10-Zwilch complex (RZZ) and Spindly to control spindle assembly checkpoint (SAC) signaling and microtubule attachment. How the ubiquitous dynein co-factors Lis1 and NudE contribute to these functions remains poorly understood. Here, we show that the C. elegans NudE homolog NUD-2 is dispensable for dynein- and LIS-1-dependent mitotic spindle assembly in the zygote. This facilitates functional characterization of kinetochore-localized NUD-2, which is recruited by the CENP-F-like proteins HCP-1 and HCP-2 independently of RZZ-Spindly and dynein-LIS-1...
January 8, 2018: Journal of Cell Science
https://www.readbyqxmd.com/read/28905497/alveolar-soft-part-sarcoma-a-case-report-with-emphasis-on-some-unusual-cytological-features
#10
Neelam Sood, Minakshi Gulia
Alveolar soft part sarcoma is a very rare, slow growing highly angiogenic tumor with poor prognosis. Most common site in children and infants is head and neck region and in adults it most commonly occurs in extremities especially thigh. In our case study, an 8 years old female patient presented with a gradually progressive left shoulder lump. FNAC from the lesion showed cellular smears with polyhedral and spindly cells showing abundant finely vacuolated cytoplasm, nuclear pleomorphism, intranuclear pseudoinclusions, and few bare nuclei...
February 2018: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/28777391/design-and-numerical-evaluation-of-an-axial-partial-assist-blood-pump-for-chinese-and-other-heart-failure-patients
#11
Guang-Mao Liu, Dong-Hai Jin, Jian-Ye Zhou, Xi-Hang Jiang, Han-Song Sun, Yan Zhang, Hai-Bo Chen, Sheng-Shou Hu, Xing-Min Gui
A fully implantable axial left ventricular assist device LAP31 was developed for Chinese or other heart failure patients who need partial support. Based on the 5-Lpm total cardiac blood output of Chinese without heart failure disease, the design point of LAP31 was set to a flow rate of 3 Lpm with 100-mmHg pressure head. To achieve the required pressure head and good hemolytic performance, a structure that includes a spindly rotor hub and a diffuser with splitter and cantilevered main blades was developed. Computational fluid dynamics (CFD) was used to analyze the hydraulic and hemodynamic performance of LAP31...
September 15, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28506934/dynamic-regulation-of-della-protein-activity-spindly-and-secret-agent-unmasked
#12
Lucie Camut, Jean-Michel Davière, Patrick Achard
No abstract text is available yet for this article.
June 5, 2017: Molecular Plant
https://www.readbyqxmd.com/read/28320825/structure-of-the-rzz-complex-and-molecular-basis-of-its-interaction-with-spindly
#13
Shyamal Mosalaganti, Jenny Keller, Anika Altenfeld, Michael Winzker, Pascaline Rombaut, Michael Saur, Arsen Petrovic, Annemarie Wehenkel, Sabine Wohlgemuth, Franziska Müller, Stefano Maffini, Tanja Bange, Franz Herzog, Herbert Waldmann, Stefan Raunser, Andrea Musacchio
Kinetochores are macromolecular assemblies that connect chromosomes to spindle microtubules (MTs) during mitosis. The metazoan-specific ≈800-kD ROD-Zwilch-ZW10 (RZZ) complex builds a fibrous corona that assembles on mitotic kinetochores before MT attachment to promote chromosome alignment and robust spindle assembly checkpoint signaling. In this study, we combine biochemical reconstitutions, single-particle electron cryomicroscopy, cross-linking mass spectrometry, and structural modeling to build a complete model of human RZZ...
April 3, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28320824/molecular-mechanism-of-dynein-recruitment-to-kinetochores-by-the-rod-zw10-zwilch-complex-and-spindly
#14
José B Gama, Cláudia Pereira, Patrícia A Simões, Ricardo Celestino, Rita M Reis, Daniel J Barbosa, Helena R Pires, Cátia Carvalho, João Amorim, Ana X Carvalho, Dhanya K Cheerambathur, Reto Gassmann
The molecular motor dynein concentrates at the kinetochore region of mitotic chromosomes in animals to accelerate spindle microtubule capture and to control spindle checkpoint signaling. In this study, we describe the molecular mechanism used by the Rod-Zw10-Zwilch complex and the adaptor Spindly to recruit dynein to kinetochores in Caenorhabditis elegans embryos and human cells. We show that Rod's N-terminal β-propeller and the associated Zwilch subunit bind Spindly's C-terminal domain, and we identify a specific Zwilch mutant that abrogates Spindly and dynein recruitment in vivo and Spindly binding to a Rod β-propeller-Zwilch complex in vitro...
April 3, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28320823/dynein-at-kinetochores-making-the-connection
#15
Toni McHugh, Julie P I Welburn
Dynein removes the checkpoint proteins from kinetochores once chromosomes are bioriented. In this issue, Gama et al. (2017. J. Cell Biol. https://doi.org/10.1083/jcb.201610108) and Mosalaganti et al. (2017. J. Cell Biol. https://doi.org/10.1083/jcb.201611060) reveal the molecular basis for how dynein and its adaptor protein Spindly are recruited to the ROD-Zw10-Zwilch complex in the fibrous corona of unattached kinetochores.
April 3, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28320419/cerebellar-ependymoma-with-overlapping-features-of-clear-cell-and-tanycytic-variants-mimicking-hemangioblastoma-a-case-report-and-literature-review
#16
REVIEW
Xiu-Peng Zhang, Yang Liu, Di Zhang, Qin Zheng, Chen Wang, Liang Wang, Qing-Chang Li, Xue-Shan Qiu, En-Hua Wang
BACKGROUND: Imaging and histology of clear-cell ependymoma and cerebellum-based hemangioblastoma are similar; distinguishing between them is a diagnostic challenge. CASE PRESENTATION: A 62-year-old Chinese woman presented with an intermittent headache of 8 years' duration. Computed tomography and magnetic resonance imaging revealed a mass in the cerebellum. Neurological imaging suggested hemangioblastoma (HB). Histologically, the tumor included cellular and paucicellular areas, in which cells were arranged in nests or diffusely distributed; and a highly vascular area, in which tumor cells were arranged in clusters and separated by capillaries...
March 20, 2017: Diagnostic Pathology
https://www.readbyqxmd.com/read/28270156/a-rapid-virus-induced-gene-silencing-vigs-method-for-assessing-resistance-and-susceptibility-to-cassava-mosaic-disease
#17
Getu Beyene, Raj Deepika Chauhan, Nigel J Taylor
BACKGROUND: Cassava mosaic disease (CMD) is a major constraint to cassava production in sub-Saharan Africa. Under field conditions, evaluation for resistance to CMD takes 12-18 months, often conducted across multiple years and locations under pressure from whitefly-mediated transmission. Under greenhouse or laboratory settings, evaluation for resistance or susceptibility to CMD involves transmission of the causal viruses from an infected source to healthy plants through grafting, or by using Agrobacterium-mediated or biolistic delivery of infectious clones...
March 7, 2017: Virology Journal
https://www.readbyqxmd.com/read/28249757/suppression-of-spindly-delays-mitotic-exit-and-exacerbates-cell-death-response-of-cancer-cells-treated-with-low-doses-of-paclitaxel
#18
Patrícia M A Silva, Nilza Ribeiro, Raquel T Lima, Cláudia Andrade, Vânia Diogo, Joana Teixeira, Cláudia Florindo, Álvaro Tavares, M Helena Vasconcelos, Hassan Bousbaa
Microtubule-targeting agents (MTAs) are used extensively for the treatment of diverse types of cancer. They block cancer cells in mitosis through the activation of the spindle assembly checkpoint (SAC), the surveillance mechanism that ensures accurate chromosome segregation at the onset of anaphase. However, the cytotoxic activity of MTAs is limited by premature mitotic exit (mitotic slippage) due to SAC silencing. Here we have explored the dual role of the protein Spindly in chromosome attachments and SAC silencing to analyze the consequences of its depletion on the viability of tumor cells treated with clinically relevant doses of paclitaxel...
May 28, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28244988/the-arabidopsis-o-fucosyltransferase-spindly-activates-nuclear-growth-repressor-della
#19
Rodolfo Zentella, Ning Sui, Benjamin Barnhill, Wen-Ping Hsieh, Jianhong Hu, Jeffrey Shabanowitz, Michael Boyce, Neil E Olszewski, Pei Zhou, Donald F Hunt, Tai-Ping Sun
Plant development requires coordination among complex signaling networks to enhance the plant's adaptation to changing environments. DELLAs, transcription regulators originally identified as repressors of phytohormone gibberellin signaling, play a central role in integrating multiple signaling activities via direct protein interactions with key transcription factors. Here, we found that DELLA is mono-O-fucosylated by the novel O-fucosyltransferase SPINDLY (SPY) in Arabidopsis thaliana. O-fucosylation activates DELLA by promoting its interaction with key regulators in brassinosteroid- and light-signaling pathways, including BRASSINAZOLE-RESISTANT1 (BZR1), PHYTOCHROME-INTERACTING-FACTOR3 (PIF3) and PIF4...
May 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/27273984/-three-dimensional-parallel-collagen-scaffold-promotes-tendon-extracellular-matrix-formation
#20
Zefeng Zheng, Weiliang Shen, Huihui Le, Xuesong Dai, Hongwei Ouyang, Weishan Chen
OBJECTIVE: To investigate the effects of three-dimensional parallel collagen scaffold on the cell shape, arrangement and extracellular matrix formation of tendon stem cells. METHODS: Parallel collagen scaffold was fabricated by unidirectional freezing technique, while random collagen scaffold was fabricated by freeze-drying technique. The effects of two scaffolds on cell shape and extracellular matrix formation were investigated in vitro by seeding tendon stem/progenitor cells and in vivo by ectopic implantation...
March 2016: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
keyword
keyword
89735
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"