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https://www.readbyqxmd.com/read/28930975/streamlined-single-cell-tcr-isolation-and-generation-of-retroviral-vectors-for-in-vitro-and-in-vivo-expression-of-human-tcrs
#1
Maran L Sprouse, Gabriele Blahnik, Thomas Lee, Natalie Tully, Pinaki Benarjee, Eddie A James, Maria J Redondo, Matthew L Bettini, Maria Bettini
Although, several methods for sequencing of paired T cell receptor (TCR) alpha and beta chains from single T cells have been developed, none so far have been conducive to downstream in vivo functional analysis of TCR heterodimers. We have developed an improved protocol based on a two-step multiplex-nested PCR, which results in a PCR product that spans entire variable regions of a human TCR alpha and beta chains. By identifying unique restriction sites and incorporating them into the PCR primers, we have made the PCR product compatible with direct sub-cloning into the template retroviral vector...
September 10, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28930052/portrayal-of-alcohol-brands-popular-among-underage-youth-on-youtube-a-content-analysis
#2
Brian A Primack, Jason B Colditz, Eva B Rosen, Leila M Giles, Kristina M Jackson, Kevin L Kraemer
OBJECTIVE: We characterized leading YouTube videos featuring alcohol brand references and examined video characteristics associated with each brand and video category. METHOD: We systematically captured the 137 most relevant and popular videos on YouTube portraying alcohol brands that are popular among underage youth. We used an iterative process to codebook development. We coded variables within domains of video type, character sociodemographics, production quality, and negative and positive associations with alcohol use...
September 2017: Journal of Studies on Alcohol and Drugs
https://www.readbyqxmd.com/read/28928711/validation-of-a-step-detection-algorithm-during-straight-walking-and-turning-in-patients-with-parkinson-s-disease-and-older-adults-using-an-inertial-measurement-unit-at-the-lower-back
#3
Minh H Pham, Morad Elshehabi, Linda Haertner, Silvia Del Din, Karin Srulijes, Tanja Heger, Matthis Synofzik, Markus A Hobert, Gert S Faber, Clint Hansen, Dina Salkovic, Joaquim J Ferreira, Daniela Berg, Álvaro Sanchez-Ferro, Jaap H van Dieën, Clemens Becker, Lynn Rochester, Gerhard Schmidt, Walter Maetzler
INTRODUCTION: Inertial measurement units (IMUs) positioned on various body locations allow detailed gait analysis even under unconstrained conditions. From a medical perspective, the assessment of vulnerable populations is of particular relevance, especially in the daily-life environment. Gait analysis algorithms need thorough validation, as many chronic diseases show specific and even unique gait patterns. The aim of this study was therefore to validate an acceleration-based step detection algorithm for patients with Parkinson's disease (PD) and older adults in both a lab-based and home-like environment...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28928688/the-role-of-verbal-instruction-and-visual-guidance-in-training-pattern-recognition
#4
Jamie S North, Ed Hope, A Mark Williams
We used a novel approach to examine whether it is possible to improve the perceptual-cognitive skill of pattern recognition using a video-based training intervention. Moreover, we investigated whether any improvements in pattern recognition transfer to an improved ability to make anticipation judgments. Finally, we compared the relative effectiveness of verbal and visual guidance interventions compared to a group that merely viewed the same sequences without any intervention and a control group that only completed pre- and post-tests...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28928019/benzoylecgonine-exposure-induced-oxidative-stress-and-altered-swimming-behavior-and-reproduction-in-daphnia-magna
#5
Marco Parolini, Beatrice De Felice, Claudia Ferrario, Noelia Salgueiro-González, Sara Castiglioni, Antonio Finizio, Paolo Tremolada
Several monitoring studies have shown that benzoylecgonine (BE) is the main illicit drug residue commonly measured in the aquatic system worldwide. Few studies have investigated the potential toxicity of this molecule towards invertebrate and vertebrate aquatic non-target organisms focusing on effects at low levels of the biological organization, but no one has assessed the consequences at higher ones. Thus, the present study was aimed at investigating the toxicity of a 48-h exposure to two concentrations of BE, similar to those found in aquatic ecosystems (0...
September 16, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28927964/behavioral-responses-of-beluga-whales-delphinapterus-leucas-to-environmental-variation-in-an-arctic-estuary
#6
Paul A Anderson, Russell B Poe, Laura A Thompson, Nansen Weber, Tracy A Romano
Some Arctic estuaries serve as substrate rubbing sites for beluga whales (Delphinapterus leucas) in the summer, representing a specialized resource for the species. Understanding how environmental variation affects the species' behavior is essential to management of these habitats in coming years as the climate changes. Spatiotemporal and environmental variables were recorded for behavioral observations, during which focal groups of whales in an estuary were video-recorded for enumeration and behavioral analysis...
September 16, 2017: Behavioural Processes
https://www.readbyqxmd.com/read/28924869/the-educational-potential-of-youtube
#7
Haley T Godwin, Murtaza Khan, Peter Yellowlees
OBJECTIVE: The objective of this paper was to examine the educational potential and effectiveness of a 3 min video clip of a simulation of schizophrenia published online at YouTube. METHOD: Researchers examined the 267 public comments published on the video-sharing website YouTube over 8 years by viewers of a schizophrenia simulation video titled "virtual hallucinations" made in the Second Life game platform. Comments were independently categorized into six groupings, then cooperatively finalized, and qualitatively analyzed...
September 18, 2017: Academic Psychiatry
https://www.readbyqxmd.com/read/28923686/high-speed-video-analysis-improves-the-accuracy-of-spinal-cord-compression-measurement-in-a-mouse-contusion-model
#8
Marion Fournely, Yvan Petit, Éric Wagnac, Jérôme Laurin, Virginie Callot, Pierre-Jean Arnoux
BACKGROUND: Animal models of spinal cord injuries aim to utilize controlled and reproducible conditions. However, a literature review reveals that mouse contusion studies using equivalent protocols may show large disparities in the observed impact force vs. cord compression relationship. The overall purpose of this study was to investigate possible sources of bias in these measurements. The specific objective was to improve spinal cord compression measurements using a video-based setup to detect the impactor-spinal cord time-to-contact...
September 15, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28923160/applying-the-neurodynamics-of-emotional-circular-causalities-in-psychosocial-and-cognitive-therapy-using-multi-sensory-environments-an-orbde-case-study-analysis
#9
Janice Ryan
This exploratory, evidence-based practice research study focuses on presenting a plausible mesoscopic brain dynamics hypothesis for the benefits of treating clients with psychosocial and cognitive challenges using a mindful therapeutic approach and multi-sensory environments. After an extensive neuroscientific review of the therapeutic benefits of mindfulness, a multi-sensory environment is presented as a window of therapeutic opportunity to more quickly and efficiently facilitate the neurobiological experience of becoming more mindful or conscious of self and environment...
October 2017: Nonlinear Dynamics, Psychology, and Life Sciences
https://www.readbyqxmd.com/read/28922395/an-analysis-of-equine-round-pen-training-videos-posted-online-differences-between-amateur-and-professional-trainers
#10
Erin Kydd, Barbara Padalino, Cathrynne Henshall, Paul McGreevy
Natural Horsemanship is popular among many amateur and professional trainers and as such, has been the subject of recent scientific enquiry. One method commonly adopted by Natural Horsemanship (NH) trainers is that of round pen training (RPT). RPT sessions are usually split into a series of bouts; each including two phases: chasing/flight and chasing offset/flight offset. However, NH training styles are heterogeneous. This study investigated online videos of RPT to explore the characteristics of RPT sessions and test for differences in techniques and outcomes between amateurs and professionals (the latter being defined as those with accompanying online materials that promote clinics, merchandise or a service to the public)...
2017: PloS One
https://www.readbyqxmd.com/read/28922312/maximum-step-length-test-performance-in-people-with-parkinson-disease-a-cross-sectional-study
#11
Ryan P Duncan, Marie E McNeely, Gammon M Earhart
BACKGROUND AND PURPOSE: The Maximum Step Length Test (MSLT), a measure of one's capacity to produce a large step, has been studied in older adults, but not in people with Parkinson disease (PD). We characterized performance and construct validity of the MSLT in PD. METHODS: Forty participants (mean age: 65.12 ± 8.20 years; 45% female) with idiopathic PD completed the MSLT while "OFF" and "ON" anti-PD medication. Construct validity was investigated by examining relationships between MSLT and measures of motor performance...
October 2017: Journal of Neurologic Physical Therapy: JNPT
https://www.readbyqxmd.com/read/28922311/motor-imagery-training-after-stroke-a-systematic-review-and-meta-analysis-of-randomized-controlled-trials
#12
Zaqueline Fernandes Guerra, Alessandra L G Lucchetti, Giancarlo Lucchetti
BACKGROUND AND PURPOSE: A number of studies have suggested that imagery training (motor imagery [MI]) has value for improving motor function in persons with neurologic conditions. We performed a systematic review and meta-analysis to assess the available literature related to efficacy of MI in the recovery of individuals after stroke. METHODS: We searched the following databases: PubMed, Web of Knowledge, Scopus, Cochrane, and PEDro. Two reviewers independently selected clinical trials that investigated the effect of MI on outcomes commonly investigated in studies of stroke recovery...
October 2017: Journal of Neurologic Physical Therapy: JNPT
https://www.readbyqxmd.com/read/28922194/benign-neonatal-sleep-myoclonus-evokes-somatosensory-responses
#13
Emma Losito, Monika Eisermann, Patricia Vignolo, Shushanik Hovhannisyan, Jean François Magny, Anna Kaminska
PURPOSE: Benign neonatal sleep myoclonus is a common nonepileptic condition occurring in neurologically normal full-term newborns. During jerks, EEG has always been described as normal. The aim of this study was to describe EEG changes associated with the myoclonic jerks. METHODS: Polygraphic video-EEG recordings of four full-term neonates presenting benign neonatal sleep myoclonus were studied. Myoclonic jerks were analyzed regarding their topography, frequency, propagation pattern, and reflex component...
September 14, 2017: Journal of Clinical Neurophysiology: Official Publication of the American Electroencephalographic Society
https://www.readbyqxmd.com/read/28922146/preliminary-clinical-evaluation-of-automated-analysis-of-the-sublingual-microcirculation-in-the-assessment-of-patients-with-septic-shock-comparison-of-automated-versus-semi-automated-software
#14
Nivin Sharawy, Ahmed Mukhtar, Sufia Islam, Reham Mahrous, Hassan Mohamed, Mohamed Ali, Amr Abd El Hakeem, Osama Hossny, Amera Refaa, Ahmed Saka, Vladimir Cerny, Sara Whynot, Ron George, Christian Lehmann
INTRODUCTION: The outcome of patients in septic shock has been shown to be related to changes within the microcirculation. Modern imaging technologies are available to generate high resolution video recordings of the microcirculation in humans. However, evaluation of the microcirculation is not yet implemented in the routine clinical monitoring of critically ill patients. This is mainly due to large amount of time and user interaction required by the current video analysis software. The aim of this study was to validate a newly developed automated method (CCTools®) for microcirculatory analysis of sublingual capillary perfusion in septic patients in comparison to standard semi-automated software (AVA3®)...
September 11, 2017: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/28922056/secondary-defects-detected-by-transmission-electron-microscopy-in-primary-ciliary-dyskinesia-diagnostics
#15
Mellisa Dixon, Amelia Shoemark
Primary ciliary dyskinesia (PCD) is predominantly an autosomal recessively inherited condition that affects ~1 in 15,000 people. Diagnosis of PCD can be complex and is ordinarily based on the results of multiple investigations. These investigations include nasal nitric oxide, high-speed video microscopy, genotyping, and electron microscopy analysis of ciliary ultrastructure. A diagnosis is ultimately confirmed by the presence of a hallmark defect identified by transmission electron microscopy or biallelic variants in a known PCD gene...
September 18, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/28920533/refinement-of-the-montreal-instrument-for-cat-arthritis-testing-for-use-by-veterinarians-detection-of-naturally-occurring-osteoarthritis-in-laboratory-cats
#16
Mary P Klinck, Beatriz P Monteiro, Bertrand Lussier, Martin Guillot, Maxim Moreau, Colombe Otis, Paulo Vm Steagall, Diane Frank, Johanne Martel-Pelletier, Jean-Pierre Pelletier, Jérôme Re Del Castillo, Eric Troncy
Objectives Feline osteoarthritis causes pain and disability. Detection and measurement is challenging, relying heavily on owner report. This study describes refinement of the Montreal Instrument for Cat Arthritis Testing, for Use by Veterinarians. Methods A video analysis of osteoarthritic (n = 6) and non-osteoarthritic (n = 4) cats facilitated expansion of scale items. Three successive therapeutic trials (using gabapentin, tramadol and oral transmucosal meloxicam spray) in laboratory cats with and without natural osteoarthritis (n = 12-20), permitted construct validation (assessments of disease status sensitivity and therapeutic responsiveness) and further scale refinements based on performance...
September 1, 2017: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/28920066/investigation-of-a-real-time-location-system-of-corneal-astigmatic-axis
#17
Jian-Guo Zhao, An-Peng Pan, Ke Zheng, A-Yong Yu
BACKGROUND: To construct a real-time computerized location system (RCLS) to analyze and display the axis of corneal astigmatism and to compare its accuracy with the Scheimpflug method. METHODS: Fifty-seven eyes of 39 volunteers with corneal astigmatism more than 1.00 diopter (D) were recruited. The RCLS was composed of a circular light-emitting diode (LED) light source, surgical microscope, surgical video system, computer and self-programming image analysis software...
2017: Eye and Vision (London, England)
https://www.readbyqxmd.com/read/28919879/automated-online-quantification-method-for-18-f-fdg-positron-emission-tomography-ct-improves-detection-of-the-epileptogenic-zone-in-patients-with-pharmacoresistant-epilepsy
#18
Vanessa Cristina Mendes Coelho, Marcia E Morita, Barbara J Amorim, Celso Darío Ramos, Clarissa L Yasuda, Helder Tedeschi, Enrico Ghizoni, Fernando Cendes
AIMS: To assess the validity of an online method to quantitatively evaluate cerebral hypometabolism in patients with pharmacoresistant focal epilepsy as a complement to the visual analysis of the (18)F-FDG positron emission tomography (PET)/CT exam. METHODS: A total of 39 patients with pharmacoresistant epilepsy and probable focal cortical dysplasia [22 patients with frontal lobe epilepsy (FLE) and 17 with temporal lobe epilepsy (TLE)] underwent a presurgical evaluation including EEG, video-EEG, MRI, and (18)F-FDG PET/CT...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28919257/seizures-by-the-clock-temporal-patterns-of-psychogenic-nonepileptic-seizures
#19
Udaya Seneviratne, Erica Minato, Eldho Paul
We hypothesized that (1) the occurrence of psychogenic nonepileptic seizures (PNES) is modulated by the interaction between the 24-hour clock and the sleep-wake cycle and (2) the pattern of modulation in PNES differs from epileptic seizures (ES). We sought to test our hypotheses in a cohort of patients diagnosed with PNES or ES in the setting of an epilepsy monitoring unit (EMU). We retrospectively reviewed consecutive video-EEG (VEEG) recordings of patients who underwent monitoring at the EMU of a tertiary hospital...
September 11, 2017: Epilepsy & Behavior: E&B
https://www.readbyqxmd.com/read/28918571/the-effect-of-communication-skills-training-on-patient-pharmacist-communication-in-pharmacy-education-a-meta-analysis
#20
REVIEW
Hye Kyung Jin, Jae Hee Choi, Ji Eun Kang, Sandy Jeong Rhie
Communication skills in pharmacy education and practice are increasingly regarded as a crucial component. However, thus far, estimating of the overall communication skills training (CST) effects in a variety of outcomes is lacking. The aim of this study was to synthesize the effects of CST in pharmacy education by performing a meta-analysis of CST studies. We searched MEDLINE, EMBASE, ERIC, CINAHL, PsycINFO, Cochrane Library, Web of Science, Communication and Mass Media Complete (CMMC), key journals, and bibliographic databases...
September 16, 2017: Advances in Health Sciences Education: Theory and Practice
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