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https://www.readbyqxmd.com/read/29044985/beneficial-effect-of-recombinant-rc1rc2-collagenases-on-human-islet-function-efficacy-of-low-dose-enzymes-on-pancreas-digestion-and-yield
#1
Gopalakrishnan Loganathan, Subhashree Venugopal, Andrew G Breite, William W Tucker, Siddharth Narayanan, Maheswaran Dhanasekaran, SriPrakash Mokshagundam, Michael L Green, Michael G Hughes, Stuart K Williams, Francis E Dwulet, Robert C McCarthy, Appakalai N Balamurugan
High number of human islets can be isolated using modern purified tissue dissociation enzymes, however it requires using >20 Wunsch Unit (WU)/gram of pancreas for digestion. Attempts to reduce this dose have resulted in pancreas under-digestion and poor islet recovery but improved islet function. In this study, we achieved high number of functional islets using low dose of recombinant collagenase enzyme mixture (RCEM). The collagenase dose used in these isolations is about 42% of the natural collagenase enzyme mixture (NCEM) dose commonly used to digest a 100g pancreas...
October 16, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28938416/pancreatic-histopathology-of-human-monogenic-diabetes-due-to-causal-variants-in-kcnj11-hnf1a-gata6-and-lmna
#2
May Sanyoura, Laura Jacobsen, David Carmody, Daniela Del Gaudio, Gorka Alkorta-Aranburu, Kelly Arndt, Ying Ying Hu, Frances Kobiernicki, Irina Kusmartseva, Mark A Atkinson, Louis H Philipson, Desmond Schatz, Martha Campbell-Thompson, Siri Atma W Greeley
Context: Monogenic diabetes is thought to account for 2% of all diabetes cases but most patients are misdiagnosed as type 1 or type 2 diabetes. To date, little is known about the histopathological features of pancreases from patients with monogenic diabetes. Objective: Retrospective study of the JDRF Network for Pancreatic Organ donors with Diabetes biorepository was used to identify possible cases with monogenic diabetes and to compare effects of genetic variants on pancreas histology...
August 16, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28926842/pharmacotherapy-with-thymoquinone-improved-pancreatic-%C3%AE-cell-integrity-and-functional-activity-enhanced-islets-revascularization-and-alleviated-metabolic-and-hepato-renal-disturbances-in-streptozotocin-induced-diabetes-in-rats
#3
Adel Galal El-Shemi, Osama Adnan Kensara, Aiman Alsaegh, Mohammed Hasan Mukhtar
AIMS: This study is aimed at evaluating the antidiabetic effects of thymoquinone (TQ) on streptozotocin (STZ)-induced diabetes in rats, and exploring the possible underlying mechanisms. METHODS: Diabetes was induced in adult male Wistar rats by intraperitoneal injection of freshly prepared STZ (65 mg/kg). After disease induction, 42 rats were equally assigned to: controls, STZ-diabetic group, and STZ-diabetic group treated with oral TQ (35 mg/kg/day) for 5 weeks...
September 20, 2017: Pharmacology
https://www.readbyqxmd.com/read/28912479/late-stage-differentiation-of-embryonic-pancreatic-%C3%AE-cells-requires-jarid2
#4
Sara Cervantes, Marta Fontcuberta-PiSunyer, Joan-Marc Servitja, Rebeca Fernandez-Ruiz, Ainhoa García, Lidia Sanchez, Young-Sook Lee, Ramon Gomis, Rosa Gasa
Jarid2 is a component of the Polycomb Repressor complex 2 (PRC2), which is responsible for genome-wide H3K27me3 deposition, in embryonic stem cells. However, Jarid2 has also been shown to exert pleiotropic PRC2-independent actions during embryogenesis. Here, we have investigated the role of Jarid2 during pancreas development. Conditional ablation of Jarid2 in pancreatic progenitors results in reduced endocrine cell area at birth due to impaired endocrine cell differentiation and reduced prenatal proliferation...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28864913/pancreatic-neuro-insular-network-in-young-mice-revealed-by-3d-panoramic-histology
#5
Shiue-Cheng Tang, Chia-Ning Shen, Pei-Yu Lin, Shih-Jung Peng, Hung-Jen Chien, Ya-Hsien Chou, Chester E Chamberlain, Pankaj J Pasricha
AIMS/HYPOTHESIS: It has been proposed that the neuro-insular network enables rapid, synchronised insulin secretion. However, to date, acquiring the pancreatic tissue map to study the neural network remains a challenging task as there is a lack of feasible approaches for large-scale tissue analysis at the organ level. Here, we have developed 3-dimensional (3D) panoramic histology to characterise the pancreatic neuro-insular network in young mice. METHODS: Pancreases harvested from young wild-type B6 mice (3 and 8 weeks old) and db/db mice (3 weeks old; db/db vs db/+) were used to develop 3D panoramic histology...
September 1, 2017: Diabetologia
https://www.readbyqxmd.com/read/28858137/ectopic-pancreatic-tissue-in-the-wall-of-the-small-intestine-two-rare-case-reports
#6
Jiannan Li, Haibin Huang, Sibo Huo, Ying Liu, Guangmeng Xu, Hongwen Gao, Kai Zhang, Tongjun Liu
RATIONALE: Ectopic pancreas, which is a kind of rare congenital disease, forms during embryonic development. It can occur throughout the whole gastrointestinal tract, but has a low tendency to develop in the wall of the small intestine. It is easy for patients with ectopic pancreases to be misdiagnosed because the symptoms are untypical and can vary. PATIENT CONCERNS: In the present study, we reported two rare cases of ectopic pancreatic tissue in the wall of the small intestine, which presented with obvious abdominal pain and distention...
September 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28852792/human-pancreatic-neuro-insular-network-in-health-and-fatty-infiltration
#7
Shiue-Cheng Tang, Luc Baeyens, Chia-Ning Shen, Shih-Jung Peng, Hung-Jen Chien, David W Scheel, Chester E Chamberlain, Michael S German
AIMS/HYPOTHESIS: Identification of a pancreatic neuro-insular network in mice suggests that a similar integration of islets and nerves may be present in the human pancreas. To characterise the neuro-insular network and the intra-pancreatic ganglia in a clinically related setting, we examined human pancreases in health and with fatty infiltration via 3-dimensional (3D) histology and compared the human pancreatic microenvironment with its counterpart in mice. METHODS: Human pancreatic specimens from individuals with normal BMI, high BMI (≥ 25) and type 2 diabetes were used to investigate the neuro-insular network...
August 29, 2017: Diabetologia
https://www.readbyqxmd.com/read/28836990/wound-healing-effect-of-euphorbia-hirta-linn-euphorbiaceae-in-alloxan-induced-diabetic-rats
#8
Riazul Haque Tuhin, Marium Begum, Sohanur Rahman, Rubaba Karim, Taslima Begum, Siraj Uddin Ahmed, Ronia Mostofa, Amir Hossain, Mohamed Abdel-Daim, Rayhana Begum
BACKGROUND: Euphorbia hirta linn., is a species of Euphorbiaceae family. They are known as asthma plant, barokhervi. The plant E. hirta is famous for its medicinal importance among the tribal population. It is a common practice to use the whole to heal wounds. Several pharmacological properties including antiseptic, anti-inflammatory, antidibetic, antispasmodic, antibacterial, antiviral, antifungal, anticonvulsant, nootropic, antifertility and aphrodisiac properties have already been reported for this plant...
August 24, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28791352/establishment-of-a-murine-pancreatic-cancer-pain-model-and-microarray-analysis-of-pain%C3%A2-associated-genes-in-the-spinal-cord-dorsal-horn
#9
Liqin Wang, Huihong Xu, Yanhu Ge, Hai Zhu, Dawei Yu, Weifeng Yu, Zhijie Lu
There is emerging evidence on the mechanisms of pancreatic cancer pain. Following the establishment of an orthotropic transplantation model of pancreatic cancer, microarray analysis was performed to identify changes in the expression levels of pain‑associated genes in the spinal cord. A mouse model of pancreatic cancer‑induced pain was established by implanting SW 1990 cells into the pancreases of female BALB/c‑nu mice. The survival rate and body weight were measured following orthotropic transplantation...
October 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28760554/pathological-and-gene-expression-analysis-of-a-polygenic-diabetes-model-noncnzo10-ltj-mice
#10
Tsuyoshi Hirata, Tomomi Yoshitomi, Minoru Inoue, Yutaka Iigo, Koji Matsumoto, Kazuishi Kubota, Akira Shinagawa
The NONcNZO10/LtJ mouse is a polygenic model of type-2 diabetes (T2D) that shows moderate obesity and diabetes, and is regarded as a good model reflective of the conditions of human T2D. In this study, we analyzed pathological changes of pancreases with the progress of time by using histopathology and gene expression analysis, including microRNA. A number of gene expression changes associated with decreased insulin secretion (possibly regulated by miR-29a/b) were observed, and zinc homeostasis (Slc30a8, Mt1 and Mt2) or glucose metabolism (Slc2a2) was suggested as being the candidate mechanism of pancreas failure in NONcNZO10/LtJ mice...
September 20, 2017: Gene
https://www.readbyqxmd.com/read/28759462/pig-islet-xenotransplantation
#11
Maheswaran Dhanasekaran, Johnathan J George, Gopalakrishnan Loganathan, Siddharth Narayanan, Michael G Hughes, Stuart K Williams, Appakalai N Balamurugan
PURPOSE OF REVIEW: The current article reviews the rationale, sources and preparation of pig islets for xenotransplantation, and presents current progress in solving the problems associated with establishing pig islet transplant as a clinical treatment for type 1 diabetes. SUMMARY: Islet transplantation is an effective treatment option for type 1 diabetes, but the available supply of human pancreases is insufficient to meet the need and demand for obtaining islets...
July 28, 2017: Current Opinion in Organ Transplantation
https://www.readbyqxmd.com/read/28678058/donors-after-circulatory-death-pancreas-transplantation
#12
Shruti Mittal, James Gilbert, Peter J Friend
PURPOSE OF REVIEW: The use of organs from donors after circulatory death (DCD) has become standard practice in solid organ transplantation of most abdominal organs and has been used successfully in some centres for pancreas transplantation. Nevertheless, concerns regarding poor graft outcomes and complications remain. This review aims to discuss the current state of DCD pancreas transplantation and the associated outcomes. RECENT FINDINGS: In many countries, whereas the number of donors after brainstem death (DBD) remains stable, the mean age and BMI have increased making these donors, previously considered to be low risk, now more marginal...
August 2017: Current Opinion in Organ Transplantation
https://www.readbyqxmd.com/read/28665321/capsaicin-sensitive-sensory-nerves-are-necessary-for-the-protective-effect-of-ghrelin-in-cerulein-induced-acute-pancreatitis-in-rats
#13
Joanna Bonior, Zygmunt Warzecha, Piotr Ceranowicz, Ryszard Gajdosz, Piotr Pierzchalski, Michalina Kot, Anna Leja-Szpak, Katarzyna Nawrot-Porąbka, Paweł Link-Lenczowski, Michał Pędziwiatr, Rafał Olszanecki, Krzysztof Bartuś, Rafał Trąbka, Beata Kuśnierz-Cabala, Artur Dembiński, Jolanta Jaworek
Ghrelin was shown to exhibit protective and therapeutic effect in the gut. Aim of the study was to investigate the role of sensory nerves (SN) in the protective effect of ghrelin in acute pancreatitis (AP). Studies were performed on male Wistar rats or isolated pancreatic acinar cells. After capsaicin deactivation of sensory nerves (CDSN) or treatment with saline, rats were pretreated intraperitoneally with ghrelin or saline. In those rats, AP was induced by cerulein or pancreases were used for isolation of pancreatic acinar cells...
June 30, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28630313/targeting-reactive-nitrogen-species-suppresses-hereditary-pancreatic-cancer
#14
Mo Li, Qian Chen, Teng Ma, Xiaochun Yu
Germline mutation of BRCA2 induces hereditary pancreatic cancer. However, how BRCA2 mutation specifically induces pancreatic tumorigenesis remains elusive. Here, we have examined a mouse model of Brca2-deficiency-induced pancreatic tumors and found that excessive reactive nitrogen species (RNS), such as nitrite, are generated in precancerous pancreases, which induce massive DNA damage, including DNA double-strand breaks. RNS-induced DNA lesions cause genomic instability in the absence of Brca2. Moreover, with the treatment of antioxidant tempol to suppress RNS, not only are DNA lesions significantly reduced, but also the onset of pancreatic cancer is delayed...
July 3, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28542139/regulator-of-g-protein-signaling-2-is-a-key-regulator-of-pancreatic-%C3%AE-cell-mass-and-function
#15
H Dong, Y Zhang, J Wang, D S Kim, H Wu, B Sjögren, W Gao, L Luttrell, H Wang
Pancreatic β-cell death and dysfunction contributes to the pathogenesis of both type 1 and type 2 diabetes. We aimed to examine whether the regulator of G protein signaling protein 2 (RGS2), a multifunctional inhibitor of G protein-coupled receptor (GPCR) signaling, impacts β-cell death and function. Metabolic phenotypes, β-cell secretory function, and glucose and insulin tolerance were measured in RGS2 knockout (RGS2(-/-)) mice and their wild-type (RGS2(+/+)) littermate controls. β-Cell death was evaluated in RGS2-knockdown and -overexpressing β cells and RGS2(-/-) islets by flow cytometry, western blot, ELISA, TUNEL staining, and apoptosis RT(2) profiler PCR array analysis...
May 25, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28537561/prediction-of-acute-pancreatitis-in-the-earliest-stages-role-of-biochemical-parameters-and-histopathological-changes
#16
Jacek Baj, Elżbieta Radzikowska, Marcin Maciejewski, Andrzej Dąbrowski, Kamil Torres
For many years, there has been a search for a set of biochemical parameters that could facilitate the assessment of severity, prognosis, and administration of early and appropriate treatment in acute pancreatitis. Administration of treatment within the first 48 hours since admission is associated with many problems of distinguishing patients with a mild form of acute pancreatitis (AP) from those with a severe form of acute pancreatitis. STUDY AIM: To assess the relationship between the extent of change in the concentration of 10 selected biochemical indicators: amylase, lipase, total bilirubin, creatinine, uric acid, aspartate transaminase, alanine transaminase, glucose, magnesium, and iron and histopathological lesions in the pancreas within 2 and 6 hours since induction of AP...
April 30, 2017: Polski Przeglad Chirurgiczny
https://www.readbyqxmd.com/read/28526921/glp-1-signalling-compensates-for-impaired-insulin-signalling-in-regulating-beta-cell-proliferation-in-%C3%AE-irko-mice
#17
Dan Kawamori, Jun Shirakawa, Chong Wee Liew, Jiang Hu, Tomoaki Morioka, Alokesh Duttaroy, Bryan Burkey, Rohit N Kulkarni
AIMS/HYPOTHESIS: We aimed to investigate potential interactions between insulin and glucagon-like peptide (GLP)-1 signalling pathways in the regulation of beta cell-cycle dynamics in vivo, in the context of the therapeutic potential of GLP-1 to modulate impaired beta cell function. METHODS: Beta cell-specific insulin receptor knockout (βIRKO) mice, which exhibit beta cell dysfunction and an age-dependent decrease in beta cell mass, were treated with the dipeptidyl peptidase-4 inhibitor vildagliptin...
August 2017: Diabetologia
https://www.readbyqxmd.com/read/28513459/effects-of-granulocyte-colony-stimulating-factor-on-opsonin-receptor-expression-and-neutrophil-antibacterial-activity-in-a-mouse-model-of-severe-acute-pancreatitis
#18
Tuo Hong-Fang, Peng Yan-Hui, Bao Lei, Zhang Wan-Xing
The antimicrobial function of neutrophils, which is dependent on opsonin receptors, deteriorates in severe acute pancreatitis (SAP). Granulocyte colony-stimulating factor (G-CSF) putatively enhanced levels of the opsonin receptors CD11b and CD32/16 in healthy human subjects, and provided protection against infection in animal models of SAP. A statistically convincing study of the effect of G-CSF on CD32/16 expression in an SAP model is lacking. We used a mouse model of SAP to investigate the association between G-CSF administration and CD32/16 levels on neutrophils and bacterial translocation...
May 9, 2017: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/28500662/application-of-a-novel-bioreactor-for-in-vivo-engineering-of-pancreas-tissue
#19
Javad Hashemi, Parvin Pasalar, Masoud Soleimani, Reza Khorramirouz, Kiarad Fendereski, Seyed Ehsan Enderami, Abdol-Mohammad Kajbafzadeh
Type 1 diabetes is characterized by autoimmune destruction of pancreatic cells. Organ transplantation is an acceptable treatment for native organ failure. However, it is associated with several problems due to a number of reasons, such as the lack of appropriate donors and immunosuppression. In our present study, a novel model is presented for in vivo recellularization of acellular pancreas by implanting between the host pancreas and the adjacent omental flap. In this study, the pancreases were harvested and cannulated via the common bile duct and then, the scaffolds were acellularized by a detergent-based protocol...
May 13, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28492724/human-islet-xenotransplantation-in-rodents-a-literature-review-of-experimental-model-trends
#20
REVIEW
Leandro Ryuchi Iuamoto, André Silva Franco, Fábio Yuji Suguita, Felipe Futema Essu, Lucas Torres Oliveira, Juliana Mika Kato, Matheus Belloni Torsani, Alberto Meyer, Wellington Andraus, Eleazar Chaib, Luiz Augusto Carneiro D'Albuquerque
Among the innovations for the treatment of type 1 diabetes, islet transplantation is a less invasive method of treatment, although it is still in development. One of the greatest barriers to this technique is the low number of pancreas donors and the low number of pancreases that are available for transplantation. Rodent models have been chosen in most studies of islet rejection and type 1 diabetes prevention to evaluate the quality and function of isolated human islets and to identify alternative solutions to the problem of islet scarcity...
April 2017: Clinics
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