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https://www.readbyqxmd.com/read/28091877/influence-of-enamel-dentin-thickness-on-the-toxic-and-esthetic-effects-of-experimental-in-office-bleaching-protocols
#1
C C de Oliveira Duque, D G Soares, F G Basso, J Hebling, C A de Souza Costa
OBJECTIVES: This paper aims to assess the whitening effectiveness and toxicity of tooth-bleaching protocols applied to enamel/dentin disks simulating mandibular incisors (ICs) and premolars (PMs). MATERIALS AND METHODS: A 10% hydrogen peroxide (H2O2) gel was applied for 3 × 15, 1 × 15, or 1 × 5 min to enamel/dentin disks simulating mandibular ICs and PMs, and the trans-enamel and trans-dentinal diffusion products were applied to human dental pulp cells (1 h)...
January 14, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28070245/horizontally-root-fractured-teeth-with-pulpal-vitality-two-case-reports
#2
Luciano Silva, Pâmella Álvares, José Alcides Arruda, Leni Verônica Silva, Cleomar Rodrigues, Ana Paula Veras Sobral, Marcia Silveira
This case study reports the successful outcome of horizontal root fractures of two different patients, which took place in permanent incisors. Report 1 describes a case of a 29-year-old patient who suffered a mandibular trauma affecting mainly the lower central incisors, caused by a car accident. A panoramic radiograph was taken right after the accident and showed a horizontal root fracture in the middle third of tooth 42, which went untreated. Report 2 illustrates a case of a 17-year-old male patient who searched for orthodontic therapy and the periapical radiograph showed horizontal root fracture in tooth 11 caused by a previous trauma, which went untreated as well...
December 28, 2016: World Journal of Radiology
https://www.readbyqxmd.com/read/28067250/promotion-of-natural-tooth-repair-by-small-molecule-gsk3-antagonists
#3
Vitor C M Neves, Rebecca Babb, Dhivya Chandrasekaran, Paul T Sharpe
The restoration of dentine lost in deep caries lesions in teeth is a routine and common treatment that involves the use of inorganic cements based on calcium or silicon-based mineral aggregates. Such cements remain in the tooth and fail to degrade and thus normal mineral volume is never completely restored. Here we describe a novel, biological approach to dentine restoration that stimulates the natural formation of reparative dentine via the mobilisation of resident stem cells in the tooth pulp. Biodegradable, clinically-approved collagen sponges are used to deliver low doses of small molecule glycogen synthase kinase (GSK-3) antagonists that promote the natural processes of reparative dentine formation to completely restore dentine...
January 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28065972/management-of-root-resorption-with-mineral-trioxide-aggregate-complicated-by-a-luxation-injury-report-of-a-case-with-six-year-follow-up
#4
Adem Kusgoz, Tahsin Yildirim, Cemile Kedici Alp, Mehmet Tanriver
Trauma to the oral region occurs frequently, comprising 5% of all injuries. The most common dental injuries are lateral luxations which can be seen with a prevalence of up to 27% among dental injuries. Ectopic eruption of maxillary canines can lead to root resorption on maxillary lateral incisors especially on apical and middle thirds of the roots. Half of these resorbed lateral incisors show severe resorption, in which the pulp is exposed. This case report describes non-surgical treatment with mineral trioxide aggregate (MTA) and a six-year follow-up of a maxillary lateral incisor, resorbed by ectopic eruption of the canine and affected by lateral luxation injury...
January 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28064220/gene-expression-analysis-of-cultured-rat-endothelial-cells-after-nd-yag-laser-irradiation-by-affymetrix-genechip-array
#5
Yoshiko Masuda, Satoshi Yokose, Hiroshi Sakagami
Endothelial cells and dental pulp cells enhance osteo-/odontogenic and angiogenic differentiation. In our previous study, rat pulp cells migrated to Nd:YAG laser-irradiated endothelial cells in an insert cell culture system. The purpose of this study was to examine the possible changes in the gene expression of cultured rat aortic endothelial cells after Nd:YAG laser irradiation using affymetrix GeneChip Array. Total RNA was extracted from the cells at 5 h after laser irradiation. Gene expressions were evaluated by DNA array chip...
January 2017: In Vivo
https://www.readbyqxmd.com/read/28058541/in-vivo-and-in-vitro-spectrophotometric-evaluation-of-upper-central-incisors-before-and-after-extraction
#6
Enric Soler, Fernando Duran-Sindreu, Juan Basilio, Miguel Roig, Stefano Ardu, Juan R Mayoral
OBJECTIVES: This in vivo and in vitro study investigated the influence of pulpal vitality on the optical properties of teeth over a 1-month period. MATERIALS AND METHODS: We monitored two specific areas (2-mm-thick pure enamel and 3-mm-thick enamel-dentine complex) in 10 teeth against two backgrounds (white and black) using a calibrated reflectance spectrophotometer at the following time points: before extraction (T0); immediately after extraction (T1); at 1-day post-extraction (T2); at 1-week post-extraction (T3); and 1-month post-extraction (T4)...
January 5, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28050518/revitalization-of-an-immature-permanent-mandibular-molar-with-a-necrotic-pulp-using-platelet-rich-fibrin-a-case-report
#7
Dayalan Subash, Krishnamma Shoba, Shibu Aman, Srinivasan Kumar Indu Bharkavi
Any insult to the pulp during its development causes cessation of dentin formation and root growth. Pulpal status and degree of root development are the decisive factors in the treatment approach. Various treatment options have been tried like surgery with root-end sealing, calcium hydroxide-apexification, placement of apical plug and regenerative endodontic procedures to induce apexogenesis. An ideal scenario for a necrosed tooth with immature root would be continued root development coupled with regeneration of pulp tissue...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28043087/long-term-treatment-outcomes-in-immature-permanent-teeth-by-revascularisation-using-mta-and-gic-as-canal-sealing-materials-a-retrospective-study
#8
Chufang Peng, Yuan Yang, Yuming Zhao, He Liu, Zheng Xu, Dianhong Zhao, Man Qin
BACKGROUND: Pulp revascularisation with MTA sealing has been accepted as an alternative treatment for non-vital immature permanent teeth. Successful revascularisation cases with GIC sealing were also reported, but lack of long-term researches. AIM: To evaluate long-term outcomes of revascularisation using MTA and GIC as canal-sealing materials in non-vital immature permanent teeth. DESIGN: Clinical and radiographic data of 60 non-vital immature permanent teeth treated with revascularisation (36...
January 2, 2017: International Journal of Paediatric Dentistry
https://www.readbyqxmd.com/read/28042276/endodontic-management-of-dens-invaginatus-type-iiib-case-series
#9
Jorge N R Martins, Rui Pereira da Costa, Craig Anderson, Sérgio André Quaresma, Luís S M Corte-Real, Adam D Monroe
Dens invaginatus may be seen as a developmental malformation. It is characterized by an invagination of the enamel and dentin, creating a lumen inside the affected tooth, which may extend as deep as the apical foramen. Oehlers Type IIIb is considered the most challenging clinical conditions. The purpose of this study is to discuss the nonsurgical endodontic management of vital and necrotic dens invaginatus Type IIIb cases. Due to the complex anatomical consideration of dens invaginatus Type IIIb, endodontic treatment is extremely technique sensitive...
October 2016: European Journal of Dentistry
https://www.readbyqxmd.com/read/28042269/clinical-evaluation-of-fiber-reinforced-composite-crowns-in-pulp-treated-primary-molars-12-month-results
#10
Zahra Mohammadzadeh, Iman Parisay, Maryam Mehrabkhani, Azam Sadat Madani, Fatemeh Mazhari
OBJECTIVE: The aim of this study was to evaluate the clinical performance of tooth-colored fiber-reinforced composite (FRC) crowns in pulp-treated second primary mandibular teeth. MATERIALS AND METHODS: This split-mouth randomized, clinical trial performed on 67 children between 3 and 6 years with two primary mandibular second molars requiring pulp treatment. After pulp therapy, the teeth were randomly assigned to stainless steel crown (SSC) or FRC crown groups...
October 2016: European Journal of Dentistry
https://www.readbyqxmd.com/read/28042259/comparison-of-compressive-strength-among-three-different-intracanal-post-materials-in-primary-anterior-teeth-an-in-vitro-study
#11
Nilavu Nilavarasan, R Hemalatha, R Vijayakumar, V S Hariharan
OBJECTIVE: The objective of our study was to compare the fracture resistance and the mode of failure among three different post materials in primary anterior teeth. MATERIALS AND METHODS: A total of sixty extracted primary anterior teeth were selected for the study. The samples were divided into three groups of twenty teeth each: Group I (Ribbond), Group II (Omega loop), and Group III (Glass fiber post). Pulp therapy was followed by intracanal post and crown buildup...
October 2016: European Journal of Dentistry
https://www.readbyqxmd.com/read/28033061/c5l2-receptor-represses-brain-derived-neurotrophic-factor-secretion-in-lipoteichoic-acid-stimulated-pulp-fibroblasts
#12
F Chmilewsky, I About, S H Chung
The anaphylatoxin C5a constitutes a powerful fragment generated by complement system activation. Interestingly, this complement active fragment is also an important mediator of tissue regeneration. Recent findings suggest that C5a could be an initial signal orchestrating pulp nerve sprouting beneath carious injury, a critical step in dentin-pulp regeneration. Indeed, the expression and activation of the C5a active receptor (C5aR/CD88) by injured pulp fibroblasts controls the direction of neurite outgrowth toward carious injuries by modulating the secretion of brain-derived neurotrophic factor (BDNF) by pulp fibroblasts...
January 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28032898/age-related-morphological-histological-and-functional-changes-in-teeth
#13
REVIEW
Thiago Saads Carvalho, Adrian Lussi
Throughout lifetime, the teeth are continuously exposed to numerous chemical and physical impacts, which cause the wear of the dental hard tissues, gingival recession and other oral changes with sometimes subsequent problems. Age-related wear of tooth surfaces reduce the dental enamel thickness and expose deeper layers of enamel, which have different physical and chemical properties than the surface enamel. Gingival recession is the main causal factor of root caries and dentine hypersensitivity. Age-related changes in dentine include the formation of secondary dentine and the reduction of tubular lumen diameter (dentine sclerosis), which lead to a reduction in the volume of the pulp chamber...
December 29, 2016: Journal of Oral Rehabilitation
https://www.readbyqxmd.com/read/28018144/deciphering-the-epigenetic-code-in-embryonic-and-dental-pulp-stem-cells
#14
REVIEW
Dashzeveg Bayarsaihan
A close cooperation between chromatin states, transcriptional modulation, and epigenetic modifications is required for establishing appropriate regulatory circuits underlying self-renewal and differentiation of adult and embryonic stem cells. A growing body of research has established that the epigenome topology provides a structural framework for engaging genes in the non-random chromosomal interactions to orchestrate complex processes such as cell-matrix interactions, cell adhesion and cell migration during lineage commitment...
December 2016: Yale Journal of Biology and Medicine
https://www.readbyqxmd.com/read/28001241/effect-of-edta-on-tgf-%C3%AE-1-released-from-the-dentin-matrix-and-its-influence-on-dental-pulp-stem-cell-migration
#15
Lidiany Freitas Gonçalves, Ana Paula Fernandes, Leopoldo Cosme-Silva, Fabio Antonio Colombo, Natália Silva Martins, Thais Marchini Oliveira, Tomaz Henrique Araujo, Vivien Thiemy Sakai
Bioactive molecules stored in dentin, such as transforming growth factor beta1 (TGF-b1), may be involved in the signaling events related to dental tissue repair. The authors conducted an in vitro evaluation of the amount of TGF-b1 released from dentin slices after treatment with 10% ethylenediaminetetraacetic acid (EDTA), 2.5% sodium hypochlorite (NaOCl) or phosphate-buffered saline (PBS), and the effect of this growth factor on stem cell migration from human exfoliated deciduous teeth (SHED). Sixty 1-mm-thick tooth slices were prepared with or without the predentin layer, and treated with either 10% EDTA for 1 minute, 2...
December 22, 2016: Brazilian Oral Research
https://www.readbyqxmd.com/read/28000851/three-dimensional-simulated-microgravity-culture-improves-the-proliferation-and-odontogenic-differentiation-of-dental-pulp-stem-cell-in-plga-scaffolds-implanted-in-mice
#16
Yanping Li, Lina He, Shuang Pan, Lin Zhang, Weiwei Zhang, Hong Yi, Yumei Niu
Tooth regeneration through stem cell-based therapy is a promising treatment for tooth decay and loss. Human dental pulp stem cells (hDPSCs) have been widely identified as the stem cells with the most potential for tooth tissue regeneration. However, the culture of hDPSCs in vitro for tissue engineering is challenging, as cells may proliferate slowly or/and differentiate poorly in vivo. Dynamic three‑dimensional (3D) simulated microgravity (SMG) created using the rotary cell culture system is considered to an effective tool, which contributes to several cell functions...
December 14, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28000152/inactivation-of-bone-morphogenetic-protein-1-bmp1-and-tolloid-like-1-tll1-in-cells-expressing-type-i-collagen-leads-to-dental-and-periodontal-defects-in-mice
#17
Hua Zhang, Priyam Jani, Tian Liang, Yongbo Lu, Chunlin Qin
Bone morphogenetic protein 1 (BMP1) and tolloid-like 1 (TLL1) belong to the BMP1/tolloid-like proteinase family, which cleaves secretory proteins. The constitutive deletion of the Bmp1 or Tll1 genes causes perinatal or embryonic lethality in mice. In this study, we first studied the β-galactosidase activity in mice in which an IRES-lacZ-Neo cassette was inserted in the intron of either the Bmp1 or the Tll1 gene; the β-galactosidase activities were used to reflect the expression of endogenous Bmp1 and Tll1, respectively...
December 20, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27989582/expression-of-cannabinoid-type-1-receptors-in%C3%A2-human-odontoblast-cells
#18
Kehua Que, Dan He, Ying Jin, Ligeng Wu, Fang Wang, Zhiying Zhao, Jing Yang, Jiayin Deng
INTRODUCTION: The aim of this study was to investigate the functional expression of cannabinoid type 1 (CB1) receptors in human odontoblasts (HODs) and the possible internal mechanism. METHODS: In the present study, we examined the molecular and functional expression of the CB1 receptors in cultured HOD-like cells and native HODs obtained from healthy wisdom teeth. RESULTS: Immunohistochemistry and immunofluorescence revealed that CB1 receptors localize to native HODs and HOD-like cells, respectively...
December 15, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27987798/transmission-electron-microscopical-study-of-teenage-crown-dentin-on-the-nanometer-scale
#19
Peter Panfilov, Anna Kabanova, Jinming Guo, Zaoli Zhang
This is the first transmission electron microscopic study of teenage crown dentin on the nanometer scale. Samples for TEM were prepared by mechanical thinning and chemical polishing that allowed obtaining the electron transparent foils. It was firstly shown that human dentin possesses the layered morphology: the layers are oriented normally to the main axis of a tooth and have the thickness of ~50nm. HA inorganic phase of teenage crown dentin is in the amorphous state. The cellular structure, which was formed from collagen fibers (diameter is ~5nm), are observed near DEJ region in teenage dentin, whereas bioorganic phase of teenage crown dentin near the pulp camera does not contain the collagen fibers...
February 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27986266/general-dentists-pediatric-dentists-and-endodontists-diagnostic-assessment-and-treatment-strategies-for-deep-carious-lesions-a-comparative-analysis
#20
Mahshid Majlessi Koopaeei, Marita R Inglehart, Neville McDonald, Margherita Fontana
BACKGROUND: There is a large variation among clinicians when managing deep carious lesions (DCLs). The purpose of this study was to assess general dentists' (GDs), pediatric dentists' (PDs), and endodontists' (EDs) diagnostic methods, clinical decision-making considerations, treatment strategies, and knowledge, behavior, and attitudes related to the diagnosis and treatment of DCLs. METHODS: A total of 175 GDs, 511 PDs, and 377 EDs responded to a nationwide Web-based survey...
December 13, 2016: Journal of the American Dental Association
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