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https://www.readbyqxmd.com/read/28214254/normothermia-in-arthroplasty
#1
REVIEW
Mark W Allen, David J Jacofsky
Since the initial design of surgical theaters, the thermal environment of the operating suite itself has been an area of concern and robust discussion. In the 1950s, correspondence in the British Medical Journal discussed the most suitable design for a surgeon's cap to prevent sweat from dripping onto the surgical field. These deliberations stimulated questions about the effects of sweat-provoking environments on the efficiency of the surgical team, not to mention the effects on the patient. Although these benefits translate to implant-based orthopedic surgery, they remain poorly understood and, at times, ignored...
January 17, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28214139/accuracy-of-computed-tomography-angiography-to-identify-thin-cap-fibroatheroma-detected-by-optical-coherence-tomography
#2
Nobuo Tomizawa, Kodai Yamamoto, Shinichi Inoh, Takeshi Nojo, Sunao Nakamura
BACKGROUND: Thin-cap fibroatheroma (TCFA) is assumed to cause acute coronary syndromes. OBJECTIVE: To compare the accuracy of different models for diagnosing TCFA using parameters derived by CT, validated against optical coherence tomography (OCT). METHODS: One hundred twenty-nine plaques in 106 patients were analyzed using data acquired by 64-row CT with a reconstruction thickness of 0.67 mm and an increment of 0.33 mm. TCFA was defined by OCT as a plaque with lipid content in ≥2 quadrants and the thinnest part of the fibrous cap measuring ≤65 μm...
February 9, 2017: Journal of Cardiovascular Computed Tomography
https://www.readbyqxmd.com/read/28213629/transcriptome-based-snp-discovery-by-gbs-and-the-construction-of-a-genetic-map-for-olive
#3
Ahmet İpek, Meryem İpek, Sezai Ercişli, Nesrin Aktepe Tangu
Molecular markers located in the genic regions of plants are valuable tools for the identification of candidate genes of economically important traits and consequent use in marker-assisted selection (MAS). In the past, simple sequence repeat markers (SSRs) and single-nucleotide polymorphisms (SNPs) located in expressed sequence tags (ESTs) were developed by sequencing RNA derived from different plant tissues, which involves laborious RNA extraction, mRNA isolation, and cDNA synthesis. In order to develop SNP markers located in olive transcriptomes, we used the recently developed genotyping-by-sequencing (GBS) technique...
February 18, 2017: Functional & Integrative Genomics
https://www.readbyqxmd.com/read/28213297/retention-of-silver-nano-particles-and-silver-ions-in-calcareous-soils-influence-of-soil-properties
#4
Samaneh Rahmatpour, Mehran Shirvani, Mohammad R Mosaddeghi, Mehdi Bazarganipour
The rapid production and application of silver nanoparticles (AgNPs) have led to significant release of AgNPs into the terrestrial environments. Once released into the soil, AgNPs could enter into different interactions with soil particles which play key roles in controlling the fate and transport of these nanoparticles. In spite of that, experimental studies on the retention of AgNPs in soils are very scarce. Hence, the key objective of this research was to find out the retention behavior of AgNPs and Ag(I) ions in a range of calcareous soils...
February 14, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28213258/p-aminothiophenol-coated-cdse-zns-quantum-dots-as-a-turn-on-fluorescent-probe-for-ph-detection-in-aqueous-media
#5
Dong Li, Hu Xu, Dan Li, Yuhong Wang
Since controlling hydrogen ion concentration (i.e. pH) is fairly important in various fields, developing a facile pH detection method is highly desired. Owing to the superior optical properties, semiconductor quantum dots (QDs) have been developed as a promising alternative for organic fluorophores in fluorescence analysis. In this study, a hydrophilic p-aminothiophenol (pATP) capped CdSe/ZnS QDs (denoted as pATP-QDs) as a selective "turn-on" fluorescence probe for pH in aqueous media is reported. The pATP-QDs probe easily obtained via ligand exchange are characterized by various analytic techniques including ultraviolet-visible, fourier transform infrared, fluorescence, time-resolved fluorescence spectroscopies, and transmission electron microscope etc...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28213095/cytotoxicity-of-gold-nanoparticles-with-different-structures-and-surface-anchored-chiral-polymers
#6
Jun Deng, Mengyun Yao, Changyou Gao
Nanoparticles (NPs) can have profound effects on cell biology. However, the potential adverse effects of gold nanoparticles (AuNPs) with different surface chirality and structures have not been elucidated. In this study, monolayers of poly(acryloyl-L(D)-valine (L(D)-PAV) chiral molecules were anchored on the surfaces of gold nanocubes (AuNCs) and nanooctahedras (AuNOs), respectively. The L-PAV-AuNCs and D-PAV-AuNCs, or the L-PAV-AuNOs and D-PAV-AuNOs, had identical physicochemical properties in terms of size, morphology and ligand density except of the reverse molecular chirality on the particle surfaces, respectively...
February 14, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28211929/calcium-phosphate-nanoparticles-prepared-from-infusion-fluids-for-stem-cell-transfection-process-optimization-and-cytotoxicity-analysis
#7
Quazi T H Shubhra, Ayako Oyane, Hiroko Araki, Maki Nakamura, Hideo Tsurushima
This is the first study to report the use of infusion fluids for particle-mediated gene delivery with DNA-immobilized calcium phosphate (CaP) nanoparticles (NPs). In conventional CaP systems, CaP NPs are fabricated in labile supersaturated CaP solutions which are prepared from chemical reagents. In the present study, we fabricated CaP NPs via coprecipitation in labile supersaturated CaP solutions that were prepared from infusion fluids (even the water used was of injectable quality) instead of chemical reagents and demonstrated their gene delivery capabilities for the hard to transfect pluripotent stem cell (C3H10T1/2) along with the easy to transfect CHO-K1 cell...
February 17, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28211915/structure-of-the-shr-scr-heterodimer-bound-to-the-bird-idd-transcriptional-factor-jkd
#8
Yoshinori Hirano, Masahiro Nakagawa, Tomoe Suyama, Kohji Murase, Maya Shirakawa, Seiji Takayama, Tai-Ping Sun, Toshio Hakoshima
The plant-specific GAI, RGA and SCR (GRAS) family proteins play critical roles in plant development and signalling. Two GRAS proteins, SHORT-ROOT (SHR) and SCARECROW (SCR), cooperatively direct asymmetric cell division and the patterning of root cell types by transcriptional control in conjunction with BIRD/INDETERMINATE DOMAIN (IDD) transcription factors, although precise details of these specific interactions and actions remain unknown. Here, we present the crystal structures of the SHR-SCR binary and JACKDAW (JKD)/IDD10-SHR-SCR ternary complexes...
February 17, 2017: Nature Plants
https://www.readbyqxmd.com/read/28210811/status-and-perception-of-oral-health-in-6-17-year-old-psychiatric-inpatients-randomized-controlled-trial
#9
I M Schüler, B Bock, R Heinrich-Weltzien, K Bekes, M Rudovsky, Ch Filz, C Ligges
AIM: The following are the aims of the study: assessment of oral health status, oral health-related quality of life (OHRQoL) and the effect of oral health care training over OHRQoL in children and adolescents hospitalized with mental disorders. METHODOLOGY: This randomized case controlled prospective interventional study involved 81 child and adolescent psychiatric inpatients (CAP) aged between 6 and 17 years (mean age 10.6 ± 2.4 years), compared to 81 mentally healthy patients attending routine dental examinations (DC group) matched according to age and sex of the CAP group...
February 16, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28210229/longitudinal-associations-between-posttraumatic-stress-disorder-severity-and-personality-disorder-features-among-female-rape-survivors
#10
Michelle J Bovin, Erika J Wolf, Patricia A Resick
This study evaluated how change in posttraumatic stress disorder (PTSD) symptoms was associated with residualized change in comorbid personality disorder (PD) features and vice versa over the course of 5-10 years. The sample was comprised of 79 female rape survivors who met criteria for PTSD and who were a part of a larger study examining the effects of trauma-focused therapy. PTSD was assessed with the fourth edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-IV) version of the Clinician-Administered PTSD Scale [CAPS-IV (1)] and PD features were assessed with the DSM-IV dimensional PD scales on the Schedule for Non-adaptive and Adaptive Personality [SNAP (2)]...
2017: Frontiers in Psychiatry
https://www.readbyqxmd.com/read/28209356/no-evidence-from-randomized-trials-to-prefer-any-direct-pulp-capping-material-over-another
#11
Romina Brignardello-Petersen
No abstract text is available yet for this article.
February 13, 2017: Journal of the American Dental Association
https://www.readbyqxmd.com/read/28209271/a-simple-method-of-extracting-cap-antigen-of-pcv2b-from-emulsified-vaccines-for-testing-its-stability-and-antigenicity
#12
Cheng Yu, Xin Li, Wenzhen Diao, Jiwei Liu, Yunpeng Yao, Li Hua, Yaqin Yu, Yongli Yu, Liying Wang
Oil-based emulsions are commonly used adjuvants for veterinary vaccines. After formulation, it is required to extract protein antigens from emulsified vaccines for testing their stability and antigenicity. To establish a simple method to extract the protein antigens, two emulsified vaccines, designated as Cap-206 and Cap-35, were prepared by formulating a 28-kDa capsid protein (Cap) of PCV2b with ISA 206, a water-in-oil-in-water emulsion, or ISA 35, an oil-in-water emulsion. We found that the freeze-thaw-centrifugation method with steps of freezing at -20 °C for 12 h, thawing at room temperature and centrifuging at 9000×g for 10 min could separate the aqueous phase from Cap-206, and a centrifugation method by centrifuging at 9000×g for 10 min could isolate the aqueous portion from Cap-35...
February 10, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28208642/photoluminescent-gold-nanoclusters-in-cancer-cells-cellular-uptake-toxicity-and-generation-of-reactive-oxygen-species
#13
Marija Matulionyte, Dominyka Dapkute, Laima Budenaite, Greta Jarockyte, Ricardas Rotomskis
In recent years, photoluminescent gold nanoclusters have attracted considerable interest in both fundamental biomedical research and practical applications. Due to their ultrasmall size, unique molecule-like optical properties, and facile synthesis gold nanoclusters have been considered very promising photoluminescent agents for biosensing, bioimaging, and targeted therapy. Yet, interaction of such ultra-small nanoclusters with cells and other biological objects remains poorly understood. Therefore, the assessment of the biocompatibility and potential toxicity of gold nanoclusters is of major importance before their clinical application...
February 10, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28206897/retrospective-study-of-the-survival-rates-of-indirect-pulp-therapy-versus-different-pulpotomy-medicaments
#14
Patrice Barsamian Wunsch, Marissa M Kuhnen, Al M Best, Tegwyn H Brickhouse
PURPOSE: The purposes of this retrospective chart review were to determine: (1) how primary molars in need of vital pulp therapy (VPT) have been treated over a period of four years at a university-based pediatric dental practice (UBP); and which treatments-indirect pulp therapy (IPT), formocresol pulpotomy (FCP), and ferric sulfate pulpotomy (FSP)-have been successful. METHODS: Electronic patient records (axiUm) that contained the procedure codes D3120 (pulp cap-indirect) or D3220 (therapeutic pulpotomy) were totaled by year...
October 15, 2016: Pediatric Dentistry
https://www.readbyqxmd.com/read/28206680/an-ssdna-aptamer-against-mannose-capped-lipoarabinomannan-enhances-anti-tuberculosis-activity-of-macrophages-through-downregulation-of-lipid-sensing-nuclear-receptor-peroxisome-proliferator-activated-receptor-%C3%AE-expression
#15
Qin Pan, Jiamin Yan, Qi Liu, Chunhui Yuan, Xiao-Lian Zhang
Mannose-capped lipoarabinomannan (ManLAM) is an immunomodulatory epitope of Mycobacterium tuberculosis (Mtb). We previously generated an aptamer (ZXL1) that specifically binds to ManLAM from the virulent Mtb H37Rv strain and reported that ZXL1 functioned as an antagonist, inhibiting the ManLAM-induced immunosuppression of dendritic cells (DCs). In the present study, we found that ZXL1 inhibited Mtb entry into murine macrophages. ZXL1 enhanced IL-1β and IL-12 mRNA expression and cytokine production in ManLAM-treated macrophages but decreased IL-10 production...
February 16, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/28205249/exploration-micromechanism-of-vp35-iid-interaction-and-recognition-dsrna-a-molecular-dynamics-simulation
#16
Yan-Jun Zhang, Jing-Na Ding, Hui Zhong, Ju-Guang Han
Multifunctional viral protein (VP35) encoded by the highly pathogenic Ebola viruses (EBOVs) can antagonize host double-stranded RNA (dsRNA) sensors and immune response because of the simultaneous recognition of dsRNA backbone and blunt ends. Mutation of select hydrophobic conserved basic residues within the VP35 inhibitory domain (IID) abrogates its dsRNA-binding activity, and impairs VP35-mediated interferon (IFN) antagonism. Herein the detailed binding mechanism between dsRNA and WT, single mutant, and double mutant were investigated by all-atom molecular dynamics (MD) simulation and binding energy calculation...
February 16, 2017: Proteins
https://www.readbyqxmd.com/read/28204846/regulation-of-end-binding-protein-eb1-in-the-control-of-microtubule-dynamics
#17
REVIEW
Anne Nehlig, Angie Molina, Sylvie Rodrigues-Ferreira, Stéphane Honoré, Clara Nahmias
The regulation of microtubule dynamics is critical to ensure essential cell functions, such as proper segregation of chromosomes during mitosis or cell polarity and migration. End-binding protein 1 (EB1) is a plus-end-tracking protein (+TIP) that accumulates at growing microtubule ends and plays a pivotal role in the regulation of microtubule dynamics. EB1 autonomously binds an extended tubulin-GTP/GDP-Pi structure at growing microtubule ends and acts as a molecular scaffold that recruits a large number of regulatory +TIPs through interaction with CAP-Gly or SxIP motifs...
February 15, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28203279/a-noninvasive-diagnostic-model-to-assess-nonalcoholic-hepatic-steatosis-in-patients-with-chronic-hepatitis-b
#18
Hongjie Ou, Shaohang Cai, Ying Liu, Muye Xia, Jie Peng
OBJECTIVE: The objective of this study was to develop a noninvasive diagnostic test for nonalcoholic hepatic steatosis in patients with chronic hepatitis B (CHB) by using routinely available clinical markers. METHODS: A retrospective study of patients with CHB, with or without hepatic steatosis (fatty change) who were diagnosed with controlled attenuation parameter (CAP) measured by transient elastography were included. Patient information was analyzed on lifestyle; laboratory tests, including serum lipid levels; blood pressure; blood uric acid; and medical history of type 2 diabetes mellitus (T2DM)...
February 2017: Therapeutic Advances in Gastroenterology
https://www.readbyqxmd.com/read/28202998/-comparative-study-of-proliferative-and-periodontal-differentiation-propensity-of-induced-pluripotent-stem-cells-at-different-passages
#19
J W Li, X H Yin, Q X Luan
OBJECTIVE: To compare the proliferative and periodontal specific differentiation abilities of induced pluripotent stem cells (iPSCs) at different passages, and to investigate whether long term culturing would have a negative influence on their proliferation and specific differentiation capacity, thus providing a theoretical basis for further in-depth research on periodontal regeneration and the possible clinical applications of iPSCs. METHODS: IPSCs derived from human gingival fibroblasts at passages 5, 10, 15 and 20 were recovered and cultured in vitro...
February 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/28202549/relationships-between-type-2-diabetes-neuropathy-and-microvascular-dysfunction-evidence-from-patients-with-cryptogenic-axonal-polyneuropathy
#20
Anna L Emanuel, Mariska D Nieuwenhoff, Erica S Klaassen, Ajay Verma, Mark H H Kramer, Rob Strijers, Alexander F J E Vrancken, Etto Eringa, Geert Jan Groeneveld, Erik H Serné
OBJECTIVE: This study investigated whether the relationship between neuropathy and microvascular dysfunction in patients with type 2 diabetes is independent of diabetes-related factors. For this purpose, we compared skin microvascular function in patients with type 2 diabetes with that of patients with cryptogenic axonal polyneuropathy (CAP), a polyneuropathy of unknown etiology. RESEARCH DESIGN AND METHODS: Cross-sectional information was collected from 16 healthy controls (HCs), 16 patients with CAP, 15 patients with type 2 diabetes with polyneuropathy (DPN), and 11 patients with type 2 diabetes without polyneuropathy...
February 15, 2017: Diabetes Care
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