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https://www.readbyqxmd.com/read/28535376/dephosphorylation-of-the-ndc80-tail-stabilizes-kinetochore-microtubule-attachments-via-the-ska-complex
#1
Dhanya K Cheerambathur, Bram Prevo, Neil Hattersley, Lindsay Lewellyn, Kevin D Corbett, Karen Oegema, Arshad Desai
During cell division, genome inheritance is orchestrated by microtubule attachments formed at kinetochores of mitotic chromosomes. The primary microtubule coupler at the kinetochore, the Ndc80 complex, is regulated by Aurora kinase phosphorylation of its N-terminal tail. Dephosphorylation is proposed to stabilize kinetochore-microtubule attachments by strengthening electrostatic interactions of the tail with the microtubule lattice. Here, we show that removal of the Ndc80 tail, which compromises in vitro microtubule binding, has no effect on kinetochore-microtubule attachments in the Caenorhabditis elegans embryo...
May 22, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28535342/diffusion-under-confinement-hydrodynamic-finite-size-effects-in-simulation
#2
Pauline Simonnin, Benoit Noetinger, Carlos Nieto Draghi, Virginie Marry, Benjamin Rotenberg
We investigate finite-size effects on diffusion in confined fluids using molecular dynamics simulations and hydrodynamic calculations. Specifically, we consider a Lennard-Jones fluid in slit pores without slip at the interface and show that the use of periodic boundary conditions in the directions along the surfaces results in dramatic finite-size effects, in addition to that of the physically relevant confining length. As in the simulation of bulk fluids, these effects arise from spurious hydrodynamic interactions between periodic images and from the constraint of total momentum conservation...
May 23, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28535056/solid-state-nmr-structure-based-inhibitor-design-to-achieve-selective-inhibition-of-the-parallel-in-register-%C3%AE-sheet-versus-antiparallel-iowa-mutant-%C3%AE-amyloid-fibrils
#3
Qinghui Cheng, Wei Qiang
Solid-state nuclear magnetic resonance (ssNMR) spectroscopy has been widely applied to characterize the high-resolution structures of β-amyloid (Aβ) fibrils. While these structures provide crucial molecular insights on the deposition of amyloid plaques in Alzheimer's diseases (AD), ssNMR structures have been rarely used so far as basis for designing inhibitors. It remains a challenge because the ssNMR-based Aβ fibrils structures were usually obtained with sparsely-isotope-labeled peptides with limited experimental constraints, where the structural models, especially the side-chain coordinates showed restricted precision...
May 23, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28534718/jasmonate-suppresses-seedling-soil-emergence-in-arabidopsis-thaliana
#4
Lulu Yao, Yuyu Zheng, Ziqiang Zhu
In addition to defense response, phytohormone jasmonate participates in various plant growth and developmental processes. Nonetheless, its role in the seedling stage is not well defined. We recently report that jasmonate suppresses hypocotyl elongation and promotes cotyledon unfolding in etiolated Arabidopsis seedlings. The molecular basis underlying this phenotype is that jasmonate treatment reduces the biochemical activity of CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) to stabilize several COP1-targetted transcription factors for eliciting a proportion of light responsive transcriptome...
May 23, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28533331/chronic-stimulation-of-renin-cells-leads-to-vascular-pathology
#5
Masafumi Oka, Silvia Medrano, Maria Luisa S Sequeira-Lόpez, R Ariel Gómez
Experimental or spontaneous genomic mutations of the renin-angiotensin system or its pharmacological inhibition in early life leads to renal abnormalities, including poorly developed renal medulla, papillary atrophy, hydronephrosis, inability to concentrate the urine, polyuria, polydipsia, renal failure, and anemia. At the core of such complex phenotype is the presence of unique vascular abnormalities: the renal arterioles do not branch or elongate properly and they have disorganized, concentric hypertrophy...
May 22, 2017: Hypertension
https://www.readbyqxmd.com/read/28533212/primitive-auxin-response-without-tir1-and-aux-iaa-in-the-charophyte-alga-klebsormidium-nitens
#6
Kinuka Ohtaka, Koichi Hori, Yuri Kanno, Mitsunori Seo, Hiroyuki Ohta
The phytohormone auxin regulates many aspects of growth and development in land plants, but the origin and evolution of auxin signaling and response mechanisms remain largely unknown. Indeed, it remains to be investigated whether auxin-related pathways diverged before the emergence of land plants. To address this knowledge deficit, we analyzed auxin responses in the charophyte alga Klebsormidium nitens NIES-2285, whose ancestor diverged from a green algal ancestor during the evolution of land plants. This strain is the same as K...
May 22, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28533186/delayed-identification-of-acinetobacter-baumannii-during-an-outbreak-owing-to-disrupted-blaoxa-51-like-by-isaba19
#7
Amjad Ahmadi, Himen Salimizand
Early detection of Acinetobacter baumannii, an emerging pathogen in hospital settings, is of interest. The blaOXA-51-like family had been used as a marker to detect A. baumannii. During an infection outbreak, 14 isolates produced a 1.7-kb PCR band instead of 353 bp for this marker. This work sought the reasons behind the increased marker size. Characterisation of isolates was done by API-20NE, gyrB multiplex PCR and 16S-23S rRNA ITS sequencing. The 1.7-kb band generated and the complete blaOXA-51-like variant were sequenced to find the probable integrated element...
May 19, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28533178/mucin-and-carbohydrate-stimulated-adhesion-and-subproteome-changes-of-the-probiotic-bacterium-lactobacillus-acidophilus-ncfm
#8
Hasan Ufuk Celebioglu, Sita Vaag Olesen, Kennie Prehn, Sampo J Lahtinen, Susanne Brix, Maher Abou Hachem, Birte Svensson
Adhesion to intestinal mucosa is a crucial property for probiotic bacteria. Adhesion is thought to increase host-bacterial interactions, thus potentially enabling health benefits to the host. Molecular events connected with adhesion and surface proteome changes were investigated for the probiotic Lactobacillus acidophilus NCFM cultured with established or emerging prebiotic carbohydrates as carbon source and in the presence of mucin, the glycoprotein of the epithelial mucus layer. Variation in adhesion to HT29-cells and mucin was associated with carbon source and mucin-induced subproteome abundancy differences...
May 19, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28533083/another-look-at-the-phylogenetic-relationships-and-intercontinental-biogeography-of-eastern-asian-north-american-rhus-gall-aphids-hemiptera-aphididae-eriosomatinae-evidence-from-mitogenome-sequences-via-genome-skimming
#9
Zhumei Ren, A J Harris, Rebecca B Dikow, Enbo Ma, Yang Zhong, Jun Wen
The Rhus gall aphids are sometimes referred to as subtribe Melaphidina (Aphididae: Eriosomatinae: Fordini) and comprise a unique group that forms galls on the primary host plants, Rhus. We examined the evolutionary relationships within the Melaphidina aphids using sequences of the complete mitochondrial genome and with samples of 11 of the 12 recognized species representing all six genera. Bayesian, maximum likelihood and parsimony analyses of the mitochondrial genome data support five well-supported clades within Melaphidina: (1) Nurudea (except N...
May 19, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28533067/production-of-poly-3-hydroxybutyrate-co-3-hydroxyvalerate-under-photoautotrophy-and-heterotrophy-by-non-heterocystous-n2-fixing-cyanobacterium
#10
Keerati Taepucharoen, Somchai Tarawat, Monthira Puangcharoen, Aran Incharoensakdi, Tanakarn Monshupanee
The photoautotrophically grown cyanobacterium Oscillatoria okeni TISTR 8549 was found to produce bioplastic poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). This PHBV production occurred under nitrogen deprivation (-N) that yielded PHBV accumulation of 14±4% (w/w DW) in which 3-hydroxyvalerate accounted for 5.5mol%. The heterotrophically grown (-N condition with acetate supplementation) cells under light showed no increase of PHBV storage, but under dark condition these cells increased PHBV accumulation to 42±8% (w/w DW) with 6...
May 6, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28532320/effects-of-light-quality-on-pod-elongation-in-soybean-glycine-max-l-merr-and-cowpea-vigna-unguiculata-l-walp
#11
Seiya Tanaka, Nobuyuki Ario, Andressa Camila Seiko Nakagawa, Yuki Tomita, Naoki Murayama, Takatoshi Taniguchi, Norimitsu Hamaoka, Mari Iwaya-Inoue, Yushi Ishibashi
Soybean pods are located at the nodes, where they are in the shadow, whereas cowpea pods are located outside of the leaves and are exposed to sunlight. To compare the effects of light quality on pod growth in soybean and cowpea, we measured the length of pods treated with white, blue, red or far-red light. In both species, pods elongated faster during the dark period than during the light period in all light treatments except red light treatment in cowpea. Red light significantly suppressed pod elongation in soybean during the dark and light periods...
May 22, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28532231/external-coincidence-model-for-hypocotyl-thermomorphogenesis
#12
Young-Joon Park, Chung-Mo Park
High but nonstressful temperatures profoundly affect plant growth and developmental processes, termed thermomorphogenesis. Thermo-induced hypocotyl elongation is a typical thermomorphogenic trait, which contributes to cooling plant organs. It is known that external light signals and the circadian clock coordinate rhythmic hypocotyl growth. However, it was unclear how light, temperature, and circadian rhythms are harmonized during hypocotyl thermomorphogenesis. We have recently demonstrated that the E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) is activated at warm temperatures...
May 22, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28532066/design-and-characterization-of-a-novel-nickel-free-cobalt-base-alloy-for-intravascular-stents
#13
Qiang Wang, Yibin Ren, M Babar Shahzad, Wei Zhang, Xumeng Pan, Song Zhang, Dan Zhang
Co-Cr-W-Ni alloy (L605) with high tensile strength is used in coronary stents. The thickness of individual strut of the stent is reduced which can decrease the stent restenosis rate. However, about 10% Ni element content in L605 is found to cause allergic reactions and pulmonary embolism, similar to the traditional 316L stainless steel. In this study, a novel nickel-free cobalt-base alloy Co-20Cr-12Fe-18Mn-2Mo-4W-N (wt%) was designed and fabricated in order to efficiently avoid the potential hazards of Ni element...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531878/effect-of-hierarchical-pore-structure-on-alp-expression-of-mc3t3-e1-cells-on-bioglass-films
#14
Cuixia Yu, Junjun Zhuang, Lingqing Dong, Kui Cheng, Wenjian Weng
Hierarchical porous bioglass films on the tantalum were designed to enhance osteointegration of metallic implants. The films were prepared by a sol-gel method using P123 as the mesopore template and polystyrene microsphere as the nanopore template. The films with 5.4nm mesopores and 100nm nanopores (MBG-100) elicited an obviously elongated morphology of the cultured MC3T3-E1 cells, as a result, a higher alkaline phosphatase level was expressed. It is suggested that the nanopores play an important role in regulating cellular behavior by initial protein adsorption through nanopore curvatures...
May 8, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28531325/up-regulated-microrna-299-corrected-with-poor-prognosis-of-glioblastoma-multiforme-patients-by-targeting-ell2
#15
Qian Huang, Xin-Wen Zhang, Yu-Shui Ma, Gai-Xia Lu, Ru-Ting Xie, Hui-Qiong Yang, Zhong-Wei Lv, Xiao-Ming Zhong, Tao Liu, Shi-Xiong Huang, Da Fu, Chun Xie
Background: An increasing understanding of the genes and molecular pathways of glioblastoma multiforme (GBM) can provide us a useful insight for the development of more effective targeted therapeutic. Methods: To investigate the expression and clinical significance of miR-299 and its target genes in GBM, the expression levels of miR-299 and its target gene in human normal brain tissues and GBM were analyzed in silico using genes microarray and hierarchical clustering analysis followed by validation with quantitative RT-PCR...
May 20, 2017: Japanese Journal of Clinical Oncology
https://www.readbyqxmd.com/read/28531235/rna-seq-analysis-of-gene-expression-for-floral-development-in-crested-wheatgrass-agropyron-cristatum-l
#16
Fangqin Zeng, Bill Biligetu, Bruce Coulman, Michael P Schellenberg, Yong-Bi Fu
Crested wheatgrass [Agropyron cristatum L. (Gaertn.)] is widely used for early spring grazing in western Canada and the development of late maturing cultivars which maintain forage quality for a longer period is desired. However, it is difficult to manipulate the timing of floral transition, as little is known about molecular mechanism of plant maturity in this species. In this study, RNA-Seq and differential gene expression analysis were performed to investigate gene expression for floral initiation and development in crested wheatgrass...
2017: PloS One
https://www.readbyqxmd.com/read/28531225/protein-charge-distribution-in-proteomes-and-its-impact-on-translation
#17
Rodrigo D Requião, Luiza Fernandes, Henrique José Araujo de Souza, Silvana Rossetto, Tatiana Domitrovic, Fernando L Palhano
As proteins are synthesized, the nascent polypeptide must pass through a negatively charged exit tunnel. During this stage, positively charged stretches can interact with the ribosome walls and slow the translation. Therefore, charged polypeptides may be important factors that affect protein expression. To determine the frequency and distribution of positively and negatively charged stretches in different proteomes, the net charge was calculated for every 30 consecutive amino acid residues, which corresponds to the length of the ribosome exit tunnel...
May 22, 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28530728/ultrafast-coulomb-explosion-of-a-diiodomethane-molecule-induced-by-an-x-ray-free-electron-laser-pulse
#18
Tsukasa Takanashi, Kosuke Nakamura, Edwin Kukk, Koji Motomura, Hironobu Fukuzawa, Kiyonobu Nagaya, Shin-Ichi Wada, Yoshiaki Kumagai, Denys Iablonskyi, Yuta Ito, Yuta Sakakibara, Daehyun You, Toshiyuki Nishiyama, Kazuki Asa, Yuhiro Sato, Takayuki Umemoto, Kango Kariyazono, Kohei Ochiai, Manabu Kanno, Kaoru Yamazaki, Kuno Kooser, Christophe Nicolas, Catalin Miron, Theodor Asavei, Liviu Neagu, Markus Schöffler, Gregor Kastirke, Xiao-Jing Liu, Artem Rudenko, Shigeki Owada, Tetsuo Katayama, Tadashi Togashi, Kensuke Tono, Makina Yabashi, Hirohiko Kono, Kiyoshi Ueda
Coulomb explosion of diiodomethane CH2I2 molecules irradiated by ultrashort and intense X-ray pulses from SACLA, the Japanese X-ray free electron laser facility, was investigated by multi-ion coincidence measurements and self-consistent charge density-functional-based tight-binding (SCC-DFTB) simulations. The diiodomethane molecule, containing two heavy-atom X-ray absorbing sites, exhibits a rather different charge generation and nuclear motion dynamics compared to iodomethane CH3I with only a single heavy atom, as studied earlier...
May 22, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28530234/n-alkylated-analogs-of-4-methylamphetamine-4-ma-differentially-affect-monoamine-transporters-and-abuse-liability
#19
Ernesto Solis, John S Partilla, Farhana Sakloth, Iwona Ruchala, Kathryn L Schwienteck, Louis J De Felice, Jose M Eltit, Richard A Glennon, S Stevens Negus, Michael H Baumann
Clandestine chemists synthesize novel stimulant drugs by exploiting structural templates known to target monoamine transporters for dopamine, norepinephrine and serotonin (DAT, NET, and SERT, respectively). 4-Methylamphetamine (4-MA) is an emerging drug of abuse that interacts with transporters, but limited structure-activity data are available for its analogs. Here we employed uptake and release assays in rat brain synaptosomes, voltage-clamp current measurements in cells expressing transporters, and calcium flux assays in cells co-expressing transporters and calcium channels to study the effects of increasing N-alkyl chain length of 4-MA on interactions at DAT, NET and SERT...
May 22, 2017: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28530160/eagle-syndrome-a-case-report-of-stylocarotid-syndrome-with-internal-carotid-artery-dissection
#20
Travis W Smoot, Ammar Taha, Nicholas Tarlov, Blake Riebe
Eagle syndrome is defined by an elongated styloid process or a calcified stylohyoid ligament that impinges on surrounding structures and causes a variety of symptoms. Classically, Eagle syndrome presents as neck, throat, or ear pain. The vascular variant, stylocarotid syndrome, can present as headache, transient ischemic attack, or stroke. Carotid artery dissection is a rare complication of stylocarotid syndrome. We report a case of stylocarotid syndrome in a 60-year-old man who presented with a right internal carotid artery (ICA) dissection and focal neurological deficits...
January 1, 2017: Interventional Neuroradiology
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