keyword
MENU ▼
Read by QxMD icon Read
search

Elongation

keyword
https://www.readbyqxmd.com/read/28926242/structure-function-studies-of-artemisia-tridentata-farnesyl-diphosphate-synthase-and-chrysanthemyl-diphosphate-synthase-by-site-directed-mutagenesis-and-morphogenesis
#1
J Scott Lee, Jian-Jung Pan, Gurusankar Ramamoorthy, C Dale Poulter
The amino acid sequences of farnesyl diphosphate synthase (FPPase) and chrysanthemyl diphosphate synthase (CPPase) from Artemisia tridentata ssp. Spiciformis, minus their chloroplast targeting regions, are 71% identical and 90% similar. FPPase efficiently and selectively synthesizes the "regular" sesquiterpenoid farnesyl diphosphate (FPP) by coupling isopentenyl diphosphate (IPP) to dimethylallyl diphosphate (DMAPP) and then to geranyl diphosphate (GPP). In contrast, CPPase is an inefficient promiscuous enzyme, which synthesizes the "irregular" monoterpenes chrysanthemyl diphosphate (CPP), lavandulyl diphosphate (LPP), and trace quantities of maconelliyl diphosphate (MPP) from two molecules of DMAPP, in addition to coupling IPP to DMAPP...
September 19, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28925971/superplastic-deformation-mechanisms-of-superfine-nanocrystalline-duplex-pm-tial-based-alloy
#2
Xuebo Gong, Zhenxin Duan, Wen Pei, Hua Chen
In this paper, the equiaxed superfine/nanocrystalline duplex PM-TiAl-based alloy with (γ + α₂) microstructure, Ti-45Al-5Nb (at %), has been synthesized by high-energy ball milling and vacuum hot pressing sintering. Superplastic deformation behavior has been investigated at 1000 °C and 1050 °C with strain rates from 5 × 10(-5) s(-1) to 1 × 10(-3) s(-1). The effects of deformation on the microstructure and mechanical behaviors of high Nb containing TiAl alloy have been characterized and analyzed. The results showed that, the ultimate tensile strength of the alloy was 58...
September 19, 2017: Materials
https://www.readbyqxmd.com/read/28925811/it-s-fun-to-transcribe-with-fun30-a-model-for-nucleosome-dynamics-during-rna-polymerase-ii-mediated-elongation
#3
Junwoo Lee, Eun Shik Choi, Daeyoup Lee
The ability of elongating RNA polymerase II (RNAPII) to regulate the nucleosome barrier is poorly understood because we do not know enough about the involved factors and we lack a conceptual framework to model this process. Our group recently identified the conserved Fun30/SMARCAD1 family chromatin-remodeling factor, Fun30(Fft3), as being critical for relieving the nucleosome barrier during RNAPII-mediated elongation, and proposed a model illustrating how Fun30(Fft3) may contribute to nucleosome disassembly during RNAPII-mediated elongation...
September 19, 2017: Transcription
https://www.readbyqxmd.com/read/28925086/optimizing-hepatic-venous-outflow-reconstruction-for-hepatic-vein-stenosis-with-indwelling-stent-in-living-donor-liver-retransplantation
#4
Takanobu Shigeta, Seisuke Sakamoto, Kengo Sasaki, Hajime Uchida, Soichi Narumoto, Akinari Fukuda, Mureo Kasahara
The patient was a boy of 7 years and 5 months of age, who underwent LDLT for acute liver failure at 10 months of age. HV stent placement was performed 8 months after LDLT because of intractable HV stenosis. At 7 years of age, his liver function deteriorated due to chronic rejection. The patient therefore underwent living donor liver retransplantation from his father. The HV was transected with the stent in situ. The IVC was resected due to stenosis. The pericardial cavity was opened and detached around the IVC to elongate the IVC...
September 18, 2017: Pediatric Transplantation
https://www.readbyqxmd.com/read/28924745/hypertonia-linked-protein-trak1-functions-with-mitofusins-to-promote-mitochondrial-tethering-and-fusion
#5
Crystal A Lee, Lih-Shen Chin, Lian Li
Hypertonia is a neurological dysfunction associated with a number of central nervous system disorders, including cerebral palsy, Parkinson's disease, dystonia, and epilepsy. Genetic studies have identified a homozygous truncation mutation in Trak1 that causes hypertonia in mice. Moreover, elevated Trak1 protein expression is associated with several types of cancers and variants in Trak1 are linked to childhood absence epilepsy in humans. Despite the importance of Trak1 in health and disease, the mechanisms of Trak1 action remain unclear and the pathogenic effects of Trak1 mutation are unknown...
September 18, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28924627/structure-and-biogenesis-of-ribonucleoprotein-bodies-in-epidermal-cells-of-caragana-arborescens-l
#6
Elizabeth Kordyum, Ninel Bilyavska
Epidermal cells of leaf petioles, pedicles, and sepals in Caragana arborescens L. are characterized with a unique biogenesis of intracellular bodies, the presence of which continues during 10-12 days in spring, from budding till flowering and fruit inception. Initially, a nuclear body is formed as a derivative of the nucleolus at the beginning of elongation of the protodermal cells, whereas a cytoplasmic body is formed in the proximity of the nuclear envelope later. Nuclear bodies and cytoplasmic bodies do not contain DNA, lipids, and starch, and they consist of RNA tightly packaged with proteins mainly in the form of short thin fibrils with thickness of 6 nm...
September 19, 2017: Protoplasma
https://www.readbyqxmd.com/read/28924119/triphasic-mitral-inflow-pattern-in-a-patient-with-congenital-absence-of-the-pericardium
#7
Masanobu Ishii, Ikuo Misumi, Tsuyoshi Honda, Taiyo Komatsu
A healthy 19-year-old man was referred to our hospital due to an elongated left heart border on chest radiograph. Transthoracic echocardiography showed a posteriorly deviated left ventricular apex, increased right ventricular dimension, and hypokinesis of the interventricular septum. Pulsed Doppler echocardiogram revealed a triphasic mitral inflow pattern. Myocardial longitudinal strain rate imaging revealed that the early diastolic strain rate abruptly decreased to a negative value and then became positive at the left ventricular apex...
September 15, 2017: Internal Medicine
https://www.readbyqxmd.com/read/28924046/in-silico-modeling-of-the-cryptic-e2-ubiquitin-binding-site-of-e6-associated-protein-e6ap-ube3a-reveals-the-mechanism-of-polyubiquitin-chain-assembly
#8
Virginia P Ronchi, Elizabeth D Kim, Christopher M Summa, Jennifer M Klein, Arthur L Haas
To understand the mechanism for assembly of Lys(48)-linked polyubiquitin degradation signals, we previously demonstrated the E6AP/UBE3A ligase harbors two functionally-distinct E2~ubiquitin binding sites: a high-affinity Site 1 required for E6AP Cys(820)~ubiquitin thioester formation and a canonical Site 2 responsible for subsequent chain elongation. Ordered binding to Sites 1 and 2 is here revealed by observation of UbcH7~ubiquitin-dependent substrate inhibition of chain formation at micromolar concentrations...
September 18, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28923978/mcam-contributes-to-the-establishment-of-cell-autonomous-polarity-in-myogenic-and-chondrogenic-differentiation
#9
Artal Moreno-Fortuny, Laricia Bragg, Giulio Cossu, Urmas Roostalu
Cell polarity has a fundamental role in shaping the morphology of cells and growing tissues. Polarity is commonly thought to be established in response to extracellular signals. Here we used a minimal in vitro assay that enabled us to monitor the determination of cell polarity in myogenic and chondrogenic differentiation in the absence of external signalling gradients. We demonstrate that the initiation of cell polarity is regulated by melanoma cell adhesion molecule (MCAM). We found highly polarized localization of MCAM, Moesin (MSN), Scribble (SCRIB) and Van-Gogh-like 2 (VANGL2) at the distal end of elongating myotubes...
September 18, 2017: Biology Open
https://www.readbyqxmd.com/read/28923837/mechanoregulation-of-clathrin-mediated-endocytosis
#10
J P Ferguson, S D Huber, N M Willy, E Aygün, S Goker, T Atabey, C Kural
We characterized the tension response of clathrin-mediated endocytosis using various cell manipulation methodologies. Elevated tension in a cell hinders clathrin-mediated endocytosis through inhibition of de novo coat initiation, elongation of clathrin coat lifetimes, and reduction of high magnitude growth rates. Actin machinery supplies an inward pulling force necessary for internalization of clathrin coats under high tension. These findings suggest that physical cues cells receive from their microenviroment are major determinants of clathrin-mediated endocytic activity...
September 18, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28923586/genetic-code-asymmetry-supports-diversity-through-experimentation-with-posttranslational-modifications
#11
REVIEW
James W Dennis
Protein N-glycosylation has been identified in all three domains of life presumably conserved for its early role in glycoprotein folding. However, the N-glycans added to proteins in the secretory pathway of multicellular organisms are remodeling in the Golgi, increasing structural diversity exponentially and adding new layers of functionality in immunity, metabolism and other systems. The branching and elongation of N-glycan chains found on cell surface receptors generates a gradation of affinities for carbohydrate-binding proteins, the galectin, selectin and siglec families...
September 15, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28923566/fabrication-of-antibacterial-chitosan-pva-blended-film-using-electrospray-technique-for-food-packaging-applications
#12
Yaowen Liu, Shuyao Wang, Wenting Lan
In this study, blended films from poly(vinyl alcohol) (PVA) containing chitosan (CS) were prepared via a simple solution casting and electrospraying method. The structures of the PVA-CS films were characterized by Fourier-transform infrared spectroscopy. The morphologies of the films were observed by scanning electron microscopy. The thermal properties of the PVA-CS films were examined by thermogravimetry. The effects of CS contents on the mechanical properties, oxygen permeability values, water vapor permeation levels, and antibacterial behaviors against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) strains were investigated...
September 15, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28923372/in-vitro-tubulogenesis-of-madin-darby-canine-kidney-mdck-spheroids-occurs-depending-on-constituent-cell-number-and-scaffold-gel-concentration
#13
Tsuyoshi Hirashima, Miho Hoshuyama, Taiji Adachi
In vitro tubulogenesis has been employed as an experimental model system to study tissue morphogenesis of internal organs. It has been previously shown that Madin-Darby canine kidney (MDCK) cells form tubes in the presence of hepatocyte growth factor in 3D cultures. Although these cells are expected to form tube structures in some microenvironments independent of chemical stimulation, little is known about the cellular mechanisms in organizing such an anisotropic multicellular structure. Here, we report 3D culture conditions that induce MDCK tubulogenesis without growth factor stimulation...
September 15, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28923030/the-eef1a2-gene-expression-as-risk-predictor-in-localized-prostate-cancer
#14
Thomas Stefan Worst, Frank Waldbillig, Abdallah Abdelhadi, Cleo-Aron Weis, Maria Gottschalt, Annette Steidler, Jost von Hardenberg, Maurice Stephan Michel, Philipp Erben
BACKGROUND: Besides clinical stage and Gleason score, risk-stratification of prostate cancer in the pretherapeutic setting mainly relies on the serum PSA level. Yet, this is associated with many uncertainties. With regard to therapy decision-making, additional markers are needed to allow an exact risk prediction. Eukaryotic translation elongation factor 1 alpha 2 (EEF1A2) was previously suggested as driver of tumor progression and potential biomarker. In the present study its functional and prognostic relevance in prostate cancer was investigated...
September 18, 2017: BMC Urology
https://www.readbyqxmd.com/read/28922745/big-brother-uncouples-cell-proliferation-from-elongation-in-the-arabidopsis-primary-root
#15
Pietro Cattaneo, Christian S Hardtke
Plant organ size is sensitive to environmental conditions, but is also limited by hardwired genetic constraints. In Arabidopsis, a few organ size regulators have been identified. Among them, the BIG BROTHER (BB) gene has a prominent role in the determination of flower organ and leaf size. BB loss-of-function mutations result in a prolonged proliferation phase during leaf(-like) organ formation, and consequently larger leaves, petals and sepals. Whether BB has a similar role in root growth is unknown. Here we describe a novel bb allele which carries a P235L point mutation in the BB RING finger domain...
September 1, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28922368/a-tree-of-life-based-on-ninety-eight-expressed-genes-conserved-across-diverse-eukaryotic-species
#16
Pawan Kumar Jayaswal, Vivek Dogra, Asheesh Shanker, Tilak Raj Sharma, Nagendra Kumar Singh
Rapid advances in DNA sequencing technologies have resulted in the accumulation of large data sets in the public domain, facilitating comparative studies to provide novel insights into the evolution of life. Phylogenetic studies across the eukaryotic taxa have been reported but on the basis of a limited number of genes. Here we present a genome-wide analysis across different plant, fungal, protist, and animal species, with reference to the 36,002 expressed genes of the rice genome. Our analysis revealed 9831 genes unique to rice and 98 genes conserved across all 49 eukaryotic species analysed...
2017: PloS One
https://www.readbyqxmd.com/read/28922057/the-dietary-food-components-capric-acid-and-caprylic-acid-inhibit-virulence-factors-in-candida-albicans-through-multitargeting
#17
Ashwini Jadhav, Supriya Mortale, Shivkrupa Halbandge, Priyanka Jangid, Rajendra Patil, Wasudev Gade, Kiran Kharat, Sankunny Mohan Karuppayil
Capric acid and caprylic acid are the dietary food components. They are found to inhibit the virulence factors like morphogenesis, adhesion, and biofilm formation in the human pathogenic yeast Candida albicans. Our study demonstrated that yeast-to-hyphal signal transduction pathways were affected by capric acid and caprylic acid. The expression profile of genes associated with serum-induced morphogenesis showed reduced expressions of Cdc35, Hwp1, Hst7, and Cph1 by the treatment with both the fatty acids. Cell elongation gene, Ece1, was surprisingly downregulated by 5208-fold by the treatment of caprylic acid...
September 18, 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28921975/intricacies-of-van-der-waals-interactions-in-systems-with-elongated-bonds-revealed-by-electron-groups-embedding-and-high-level-coupled-cluster-approaches
#18
Ewa Pastorczak, Jun Shen, Michal Hapka, Piotr Piecuch, Katarzyna Pernal
Non-covalent interactions between molecules with stretched intramonomer covalent bonds are a fascinating, yet little studied area. This shortage of information stems largely from the inability of most of the commonly used computational quantum chemistry methods to accurately describe weak long-range and strong non-dynamic correlations at the same time. In this work, we propose a geminal-based approach, abbreviated as EERPA-GVB, capable of describing such systems in a robust manner using relatively inexpensive computational steps...
September 18, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28921532/the-morphological-characterization-of-orientation-biased-displaced-large-field-ganglion-cells-in-the-central-part-of-goldfish-retina
#19
Hideo Hoshi, Fumi Sato
The vertebrate retina has about 30 subtypes of ganglion cells. Each ganglion cell receives synaptic inputs from specific types of bipolar and amacrine cells ramifying at the same depth of the inner plexiform layer (IPL), each of which is thought to process a specific aspect of visual information. Here, we identified one type of displaced ganglion cell in the goldfish retina which had a large and elongated dendritic field. As a population, all of these ganglion cells were oriented in the horizontal axis and perpendicular to the dorsal-ventral axis of the goldfish eye in the central part of retina...
September 18, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/28920779/ocular-findings-and-select-ophthalmic-diagnostic-tests-in-captive-american-white-pelicans-pelecanus-erythrorhynchos
#20
Matthew E Kinney, Aaron C Ericsson, Craig L Franklin, Rebecca E H Whiting, Jacqueline W Pearce
The aim of this study was to establish normal ophthalmic parameters for select diagnostic tests in American white pelicans (Pelecanuserythrorhynchos). Twenty-one zoo-housed American white pelicans were manually restrained for noninvasive ocular diagnostic testing and complete ophthalmic examination. Tear production quantification using the phenol red thread test (PRTT), fluorescein staining, and intraocular pressure (IOP) evaluation were performed. In addition, conjunctival aerobic bacterial culture and culture-independent 16S rRNA amplicon sequencing were performed on select eyes...
September 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
keyword
keyword
89623
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"