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https://www.readbyqxmd.com/read/29792724/a-systematic-understanding-of-signaling-by-erbb2-in-cancer-using-phosphoproteomics
#1
C Sidhanth, P Manasa, S Krishnapriya, S Sneha, S Bindhya, R P Nagare, M Garg, T S Ganesan
ErbB2 is an important receptor tyrosine kinase and a member of the ErbB family. Although it does not have a specific ligand, it transmits signals downstream by heterodimerization with other receptors in the family. It plays a major role in a variety of cellular responses like proliferation, differentiation, and adhesion. ErbB2 is amplified at the DNA level in breast cancer (20%-30%) and gastric cancer (10%-20%), and trastuzumab is effective as a therapeutic antibody. This review is a critical analysis of the currently published data on the signaling pathways of ErbB2 and the interacting proteins...
May 24, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/29788173/overexpression-of-golgi-phosphoprotein-2-is-associated-with-poor-prognosis-in-oral-squamous-cell-carcinoma
#2
Hao Li, Lei-Lei Yang, Yao Xiao, Wei-Wei Deng, Lei Chen, Lei Wu, Wen-Feng Zhang, Zhi-Jun Sun
Objectives: The aims of this study were to investigate the relationship between Golgi phosphoprotein 2 (GOLPH2) and oral squamous cell carcinoma (OSCC) and explore the clinical significance of GOLPH2 in OSCC. Methods: Tissue microarrays from human OSCC samples were stained for GOLPH2 expression and clinicopathologic features. Kaplan-Meier analysis was used to compare the survival of patients with high GOLPH2 expression and patients with low GOLPH2 expression. Results: We found GOLPH2 is highly expressed in OSCC tissue, and the GOLPH2 expression in metastatic lymph nodes is higher than in tumor tissue...
May 16, 2018: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29787783/segmentation-of-the-rabies-virus-genome
#3
Yuya Hidaka, Chang-Kweng Lim, Mutsuyo Takayama-Ito, Chun-Ho Park, Kazunori Kimitsuki, Nozomi Shiwa, Ken-Ichi Inoue, Takuya Itou
We established a system for the recovery of a segmented recombinant rabies virus, the virus genome RNA of which was divided into two parts: segment 1 encoding the nucleoprotein, phosphoprotein, matrix protein, and glycoprotein genes, and segment 2 encoding the large RNA-dependent RNA polymerase gene. The morphology of the segmented recombinant rabies virus was bullet-like in shape with a length of approximately 130 nm, which is shorter than the 200-nm long non-segmented recombinant rabies virus. The segmented recombinant rabies virus was maintained for at least 18 passages...
May 19, 2018: Virus Research
https://www.readbyqxmd.com/read/29784711/quantitative-proteome-and-phosphoproteome-analyses-of-streptomyces-coelicolor-reveal-proteins-and-phosphoproteins-modulating-differentiation-and-secondary-metabolism
#4
Beatriz Rioseras, Pavel V Sliaha, Vladimir Gorshkov, Paula Yagüe, Maria T López-García, Nathaly González-Quiñónez, Sergey Kovalchuk, Adelina Rogowska-Wrzesinska, Ole N Jensen, Angel Manteca
Streptomycetes are multicellular bacteria with complex developmental cycles. They are of biotechnological importance as they produce most bioactive compounds used in biomedicine, e.g. antibiotic, antitumoral and immunosupressor compounds. Streptomyces genomes encode a large number of Ser/Thr/Tyr kinases, making this genus an outstanding model for the study of bacterial protein phosphorylation events. We used mass spectrometry based quantitative proteomics and phosphoproteomics to characterize bacterial differentiation and activation of secondary metabolism of Streptomyces coelicolor...
May 21, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29782621/darpp-32-and-t-darpp-promote-non-small-cell-lung-cancer-growth-through-regulation-of-ikk%C3%AE-dependent-cell-migration
#5
Sk Kayum Alam, Matteo Astone, Ping Liu, Stephanie R Hall, Abbygail M Coyle, Erin N Dankert, Dane K Hoffman, Wei Zhang, Rui Kuang, Anja C Roden, Aaron S Mansfield, Luke H Hoeppner
Lung cancer is the leading cause of cancer-related death worldwide. Here we demonstrate that elevated expression of dopamine and cyclic adenosine monophosphate-regulated phosphoprotein, Mr 32000 (DARPP-32) and its truncated splice variant t-DARPP promote lung tumor growth, while abrogation of DARPP-32 expression in human non-small cell lung cancer (NSCLC) cells reduces tumor growth in orthotopic mouse models. We observe a novel physical interaction between DARPP-32 and inhibitory kappa B kinase-α (IKKα) that promotes NSCLC cell migration through non-canonical nuclear factor kappa-light-chain-enhancer of activated B cells 2 (NF-κB2) signaling...
2018: Communications biology
https://www.readbyqxmd.com/read/29781405/changes-of-hippocampus-proteomic-profiles-after-blueberry-extracts-supplementation-in-app-ps1-transgenic-mice
#6
Hai-Qiang Li, Long Tan, Hong-Peng Yang, Wei Pang, Tong Xu, Yu-Gang Jiang
OBJECTIVE: To examine protein changes in the hippocampus of APP/PS1 transgenic mice after blueberry extracts (BB) intervention. METHODS: Eight APP/PS1 transgenic mice were randomly assigned to Alzheimer's disease (AD)+BB group (n=4) and AD+control group (n=4). After a 16-week treatment, 2-DE and MALDI-TOF-MS were used to compare the proteomic profiles of the hippocampus in the two groups and Western blot was used to confirm the important differentially expressed proteins...
May 21, 2018: Nutritional Neuroscience
https://www.readbyqxmd.com/read/29781146/modulation-of-serine-threonine-phosphatases-by-melatonin-therapeutic-approaches-in-neurodegenerative-diseases
#7
REVIEW
Raquel L Arribas, Alejandro Romero, Javier Egea, Cristóbal de Los Ríos
Melatonin is an endogenous hormone produced by the pineal gland as well as many other tissues and organs. The natural decline in melatonin levels with aging strongly contributes to the development of neurodegenerative disorders. Neurodegenerative diseases share common mechanisms of toxicity such as proteinopathy, mitochondrial dysfunction, metal dyshomeostasis, oxidative stress, neuroinflammation, and an imbalance in the phosphorylation/dephosphorylation ratio. Several reports have proved the usefulness of melatonin in counteracting the events that lead to a neurodegenerative scenario...
May 20, 2018: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29769430/oral-lactobacilli-and-salivary-acidic-proline-rich-proteins-aprp-1-2-in-dental-caries
#8
A K Szkaradkiewicz-Karpinska, A Zeidler, O Goslinska-Kuzniarek, K Uram, A Szkaradkiewicz
A previous study shows that levels of acidic salivary proline-rich phosphoproteins-1/2 (APRP-1/2) increase with caries severity. The aim of this study was to examine whether this relationship also depends on the presence of H2 O2 -producing strains of Lactobacillus spp. Adults with severe caries (decayed, missing, and filled teeth (DMFT) > 13.9, n = 28) were compared with similarly aged adults who had minimal caries (DMFT < 5, n = 20). A total of 48 samples of whole unstimulated saliva were collected in the morning and centrifuged...
February 2018: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/29764989/global-assessment-of-its-network-dynamics-reveals-that-the-kinase-plk1-inhibits-the-phosphatase-pp6-to-promote-aurora-a-activity
#9
Arminja N Kettenbach, Kate A Schlosser, Scott P Lyons, Isha Nasa, Jiang Gui, Mark E Adamo, Scott A Gerber
Polo-like kinase 1 (Plk1) is an essential protein kinase that promotes faithful mitotic progression in eukaryotes. The subcellular localization and substrate interactions of Plk1 are tightly controlled and require its binding to phosphorylated residues. To identify phosphorylation-dependent interactions within the Plk1 network in human mitotic cells, we performed quantitative proteomics on HeLa cells cultured with kinase inhibitors or expressing a Plk1 mutant that was deficient in phosphorylation-dependent substrate binding...
May 15, 2018: Science Signaling
https://www.readbyqxmd.com/read/29763674/septin-associated-protein-kinase-gin4-affects-localization-and-phosphorylation-of-chs4-the-regulatory-subunit-of-the-baker-s-yeast-chitin-synthase-iii-complex
#10
Simon Gohlke, Daniela Heine, Hans-Peter Schmitz, Hans Merzendorfer
Chitin is mainly formed by the chitin synthase III complex (CSIII) in yeast cells. This complex is considered to be composed of the catalytic subunit Chs3 and the regulatory subunit Chs4, both of which are phosphoproteins and transported to the plasma membrane by different trafficking routes. During cytokinesis, Chs3 associates with Chs4 and other proteins at the septin ring, which results in an active CSIII complex. In this study, we focused on the role of Chs4 as a regulatory subunit of the CSIII complex...
May 12, 2018: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/29761507/upregulation-of-the-adipokine-genes-adipor1-and-spp1-is-related-to-poor-survival-outcomes-in-colorectal-cancer
#11
Eun K Choe, Jin W Yi, Young J Chai, Kyu J Park
BACKGROUND: Obesity is closely associated with colorectal cancer (CRC), but the underlying mechanism is unclear. We thus evaluated the expression of the adipokine gene family in CRC tissues and its clinicopathological implications. METHODS: Correlations between the mRNA expression levels of the adipokine gene family (ADIPOQ, ADIPOR1/2, LEP, LEPR, RETN, RETNLB, RBP4, SFRP5, NAMPT, and SPP1) in CRC tissue and clinicopathologic factors were analyzed using data from The Cancer Genome Atlas database...
May 14, 2018: Journal of Surgical Oncology
https://www.readbyqxmd.com/read/29759185/a-fast-sample-processing-strategy-for-large-scale-profiling-of-human-urine-phosphoproteome-by-mass-spectrometry
#12
Xinyuan Zhao, Wanjun Zhang, Tong Liu, Hangyan Dong, Junjie Huang, Changqing Sun, Guangshun Wang, Xiaohong Qian, Weijie Qin
Liquid biopsies using body fluids have gained much attention in recent years due to their multiple advantages in clinical diagnosis, such as less/non-invasive collection, suitability for longitudinal disease monitoring, and better representation of tumor heterogeneity. As an attractive choice for liquid biopsy, urine proteins and their post-translational modifications (PTMs) have the potential to offer significant insights into physiological variations and pathological changes in the human body. However, due to the intrinsically large variability of urine proteins and their PTMs among different individuals, there is a high demand for strategies for high-throughput analysis of a large number of samples to obtain a comprehensive view and a reliable reference interval of the urine proteome...
August 1, 2018: Talanta
https://www.readbyqxmd.com/read/29749514/proteomic-analysis-of-chick-retina-during-early-recovery-from-lens%C3%A2-induced-myopia
#13
Yun Yun Zhou, Rachel Ka Man Chun, Jian Chao Wang, Bing Zuo, King Kit Li, Thomas Chuen Lam, Quan Liu, Chi-Ho To
Myopia development has been extensively studied from different perspectives. Myopia recovery is also considered important for understanding the development of myopia. However, despite several previous studies, retinal proteomics during recovery from myopia is still relatively unknown. Therefore, the aim of the present study was to investigate the changes in protein profiles of chicken retinas during early recovery from lens‑induced myopia to evaluate the signals involved in the adjustment of this refractive disorder...
May 3, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29749054/dehydration-responsive-nuclear-proteome-landscape-of-chickpea-cicer-arietinum-l-reveals-phosphorylation-mediated-regulation-of-stress-response
#14
Pragya Barua, Nilesh Vikram Lande, Pratigya Subba, Dipak Gayen, Sneha Pinto, T S Keshav Prasad, Subhra Chakraborty, Niranjan Chakraborty
Non-availability of water or dehydration remains recurring climatic disorder affecting yield of major food crops, legumes in particular. Nuclear proteins (NP) and phosphoproteins (NPPs) execute crucial cellular functions that form the regulatory hub for coordinated stress response. Phosphoproteins hold enormous influence over cellular signalling. Four-week-old seedlings of a grain legume, chickpea, were subjected to gradual dehydration and nuclear proteins were extracted from unstressed control as well as from 72 and 144 h stressed tissues...
May 10, 2018: Plant, Cell & Environment
https://www.readbyqxmd.com/read/29745816/the-unique-biomineralization-transcriptome-and-proteome-of-lytechinus-variegatus-teeth
#15
Keith Alvares, Caroline J DeHart, Paul M Thomas, Neil L Kelleher, Arthur Veis
BACKGROUND: Matrix-regulated biomineralization involves the specific nucleation and growth of mineral phases within or upon preformed structured organic matrices. We hypothesized that there might be a general mechanism whereby anionic, phosphorylated mineral ion-binding proteins assist in specifically locating the mineral ions with respect to the mineralizing structural organic matrix. Here we extended these studies to invertebrate mineralization in Lytechinus variegatus (Lv) teeth. MATERIALS AND METHODS: The tooth proteins were extracted and the phosphoproteins occluded in the mineral were enriched by passage through a ProQ Diamond phosphoprotein enrichment column, and subjected to MS/MS analysis...
December 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29745025/dentin-phosphoprotein-mimetic-peptide-nanofibers-promote-biomineralization
#16
Gulcihan Gulseren, Gulistan Tansik, Ruslan Garifullin, Ayse B Tekinay, Mustafa O Guler
Dentin phosphoprotein (DPP) is a major component of the dentin matrix playing crucial role in hydroxyapatite deposition during bone mineralization, making it a prime candidate for the design of novel materials for bone and tooth regeneration. The bioactivity of DPP-derived proteins is controlled by the phosphorylation and dephosphorylation of the serine residues. Here an enzyme-responsive peptide nanofiber system inducing biomineralization is demonstrated. It closely emulates the structural and functional properties of DPP and facilitates apatite-like mineral deposition...
May 10, 2018: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29725363/overexpression-of-csk-binding-protein-phosphoprotein-associated-with-glycosphingolipid-enriched-microdomains-induces-cluster-of-differentiation-59-mediated-apoptosis-in-jurkat-cells
#17
Bei-Bei Cong, Mei-Hua Gao, Bing Li, Bing Wang, Bei Zhang, Li-Na Wang, Shu-Chao Zhang, Hua-Qiao Li, Zhong Wang, Shu-Yi Han
Csk-binding protein/phosphoprotein associated with glycosphingolipid-enriched microdomains (CBP/PAG) is a membrane-bound adaptor protein that downregulates the activation of Src family kinases present in lipid rafts. To elucidate the role of CBP/PAG in human T cell activation, a cell line overexpressing CBP/PAG was constructed and the function of CBP/PAG in Jurkat cells was examined. The present study revealed that increased CBP/PAG expression in T cells significantly enhanced their apoptosis and reduced cellular activation and proliferation...
May 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29725012/nucleolar-trf2-attenuated-nucleolus-stress-induced-hcc-cell-cycle-arrest-by-altering-rrna-synthesis
#18
Fuwen Yuan, Chenzhong Xu, Guodong Li, Tanjun Tong
The nucleolus is an important organelle that is responsible for the biogenesis of ribosome RNA (rRNA) and ribosomal subunits assembly. It is also deemed to be the center of metabolic control, considering the critical role of ribosomes in protein translation. Perturbations of rRNA synthesis are closely related to cell proliferation and tumor progression. Telomeric repeat-binding factor 2 (TRF2) is a member of shelterin complex that is responsible for telomere DNA protection. Interestingly, it was recently reported to localize in the nucleolus of human cells in a cell-cycle-dependent manner, while the underlying mechanism and its role on the nucleolus remained unclear...
May 3, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29720836/identification-of-potential-anticancer-protein-targets-in-cytotoxicity-mediated-by-tropical-medicinal-fern-extracts
#19
Siok-Thing Tan, Hean-Chooi Ong, Tsun-Thai Chai, Fai-Chu Wong
Background: Medicinal fern species represent a potentially important source for both food and medicinal applications. Previously, two underutilized tropical fern species (Blechnum orientale and Phymatopteris triloba) were reported with cytotoxic activities against selected cancer cell lines. However, the exact mechanism remains elusive. Objective: In this paper, we reported the identification of six differentially expressed proteins isolated from cancer cells, following exposure to the cytotoxic fern extracts...
April 2018: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29718670/evaluation-of-nci-7-cell-line-panel-as-a-reference-material-for-clinical-proteomics
#20
David J Clark, Yingwei Hu, William Bocik, Lijun Chen, Michael Schnaubelt, Rhonda Roberts, Punit Shah, Gordon Whiteley, Hui Zhang
Reference materials are vital to benchmarking the reproducibility of clinical tests and essential for monitoring laboratory performance for clinical proteomics. The reference material utilized for mass spectrometric analysis of the human proteome would ideally contain enough proteins to be suitably representative of the human proteome, as well as exhibit a stable protein composition in different batches of sample regeneration. Previously, The Clinical Proteomic Tumor Analysis Consortium (CPTAC) utilized a PDX-derived comparative reference (CompRef) materials for the longitudinal assessment of proteomic performance; however, inherent drawbacks of PDX-derived material, including extended time needed to grow tumors and high level of expertise needed, have resulted in efforts to identify a new source of CompRef material...
May 8, 2018: Journal of Proteome Research
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