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https://www.readbyqxmd.com/read/28747907/membrane-phosphoproteomics-of-yeast-early-response-to-acetic-acid-role-of-hrk1-kinase-and-lipid-biosynthetic-pathways-in-particular-sphingolipids
#1
Joana F Guerreiro, Nuno P Mira, Aline X S Santos, Howard Riezman, Isabel Sá-Correia
Saccharomyces cerevisiae response and tolerance to acetic acid is critical in industrial biotechnology and in acidic food and beverages preservation. The HRK1 gene, encoding a protein kinase of unknown function belonging to the "Npr1-family" of kinases known to be involved in the regulation of plasma membrane transporters, is an important determinant of acetic acid tolerance. This study was performed to identify the alterations occurring in yeast membrane phosphoproteome profile during the adaptive early response to acetic acid stress (following 1 h of exposure to a sub-lethal inhibitory concentration; 50 mM at pH 4...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28740101/p38-mapk-signaling-and-phosphorylations-in-the-brct1-domain-regulate-xrcc1-recruitment-to-sites-of-dna-damage
#2
Mirta Mittelstedt Leal de Sousa, Karine Øian Bjørås, Audun Hanssen-Bauer, Karin Solvang-Garten, Marit Otterlei
XRCC1 is a scaffold protein involved in base excision repair and single strand break repair. It is a phosphoprotein that contains more than 45 phosphorylation sites, however only a few of these have been characterized and connected to specific kinases and functions. Mitogen activated protein kinases (MAPK) are mediators of cellular stress responses, and here we demonstrate that p38 MAPK signaling is involved in phosphorylation of XRCC1 and regulation of recruitment to oxidative stress. Inhibition of p38 MAPK caused a marked pI shift of XRCC1 towards a less phosphorylated state...
July 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28739728/ezrin-radixin-moesin-binding-phosphoprotein-50-ebp50-suppresses-the-metastasis-of-breast-cancer-and-hela-cells-by-inhibiting-matrix-metalloproteinase-2-activity
#3
Lei Wang, Yijun Qi, Ying Xiong, Zhiqiang Peng, Qiang Ma, Yue Zhang, Jian Song, Junfang Zheng
BACKGROUND: Expression of ezrin-radixin-moesin-binding phosphoprotein-50 (EBP50) is correlated with human breast and cervical cancer development, but its effects on the metastasis of breast and cervical cancer and the underlying mechanism are not fully understood. MATERIALS AND METHODS: In this study, EBP50 was overexpressed in MDA-MB-231 breast cancer and HeLa cervical cancer cells; moreover, EBP50 was knocked-down in MCF-7 breast cancer cells and HeLa cells. Metastasis-related ability and matrix metalloproteinase-2 (MMP-2) activity of these cells were investigated...
August 2017: Anticancer Research
https://www.readbyqxmd.com/read/28738126/comparing-plasma-phospho-tau-total-tau-and-phospho-tau-total-tau-ratio-as-acute-and-chronic-traumatic-brain-injury-biomarkers
#4
Richard Rubenstein, Binggong Chang, John K Yue, Allen Chiu, Ethan A Winkler, Ava M Puccio, Ramon Diaz-Arrastia, Esther L Yuh, Pratik Mukherjee, Alex B Valadka, Wayne A Gordon, David O Okonkwo, Peter Davies, Sanjeev Agarwal, Fan Lin, George Sarkis, Hamad Yadikar, Zhihui Yang, Geoffrey T Manley, Kevin K W Wang, Shelly R Cooper, Kristen Dams-O'Connor, Allison J Borrasso, Tomoo Inoue, Andrew I R Maas, David K Menon, David M Schnyer, Mary J Vassar
Importance: Annually in the United States, at least 3.5 million people seek medical attention for traumatic brain injury (TBI). The development of therapies for TBI is limited by the absence of diagnostic and prognostic biomarkers. Microtubule-associated protein tau is an axonal phosphoprotein. To date, the presence of the hypophosphorylated tau protein (P-tau) in plasma from patients with acute TBI and chronic TBI has not been investigated. Objective: To examine the associations between plasma P-tau and total-tau (T-tau) levels and injury presence, severity, type of pathoanatomic lesion (neuroimaging), and patient outcomes in acute and chronic TBI...
July 24, 2017: JAMA Neurology
https://www.readbyqxmd.com/read/28734226/inhibition-of-prb-pathway-differentially-modulates-apoptosis-in-esophageal-cancer-cells
#5
Rossana C Soletti, Deborah Biasoli, Nathassya A L V Rodrigues, João M A Delou, Renata Maciel, Vera L A Chagas, Rodrigo A P Martins, Stevens K Rehen, Helena L Borges
Esophageal cancer is the sixth most common cause of cancer-related death worldwide. Current chemotherapy regimens include a combination of 5-fluorouracil (5-FU) and cisplatin, but more efficient therapy strategies are needed to increase 5-year survival. Alterations in the signaling pathway of the tumor suppressor gene Rb-1, which encodes a phosphoprotein (pRB) that negatively regulates the G1/S transition of the cell cycle, are present in 70% of all tumors, but its role in esophageal cancer is still unclear...
July 19, 2017: Translational Oncology
https://www.readbyqxmd.com/read/28733543/the-role-of-adrenomedullin-in-the-pathogenesis-of-gastric-cancer
#6
Fuhao Qiao, Jian Fang, Jinfeng Xu, Wenqiu Zhao, Ying Ni, Bufugdi Andreas Akuo, Wei Zhang, Yun Liu, Fangfang Ding, Guanlin Li, Baoguo Liu, Hua Wang, Shihe Shao
Adrenomedullin has been shown to be overexpressed in many tumors, including gastric cancer tumors; however, its mechanism of action remains unclear. In this study, we examined the role of adrenomedullin in the pathogenesis of gastric cancer. Using clinical specimens and immunohistochemistry, we found that the expression levels of adrenomedullin and its receptors are inordinately elevated as compared to the adjacent non-tumor gastric tissues. We used siRNA gene silencing, in BGC-823 gastric cancer cell lines, to target adrenomedullin genes, and found that increased adrenomedullin expression results in the proliferation of tumor cells, tumor invasion, and metastasis...
June 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28733522/abi3-a-component-of-the-wave2-complex-is-potentially-regulated-by-pi3k-akt-pathway
#7
Lais Moraes, Nilson I T Zanchin, Janete M Cerutti
We previously reported that ABI3 expression is lost in follicular thyroid carcinomas and its restoration significantly inhibited cell growth, invasiveness, migration, and reduced tumor growth in vivo. The mechanistic basis by which ABI3 exerts its tumor suppressive effects is not fully understood. In this study, we show that ABI3 is a phosphoprotein. Using proteomic array analysis, we showed that ABI3 modulated distinct cancer-related pathways in thyroid cancer cells. The KEA analysis found that PI3K substrates were enriched and forced expression of ABI3 markedly decreased the phosphorylation of AKT and the downstream-targeted protein pGSK3β...
June 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28731192/anp32a-modulates-cell-growth-by-regulating-p38-and-akt-activity-in-colorectal-cancer
#8
Wei Yan, Zhun Bai, Juan Wang, Xumei Li, Bixia Chi, Xu Chen
Acidic leucine-rich nuclear phosphoprotein-32A (ANP32A) possesses multiple biochemical activities, has been found to be decreased or absent in malignant tumors. However, new findings have shown that it is expressed in greater amounts in advanced cancers than in early-stage tumors. The role and clinical significance of ANP32A in colorectal cancer (CRC) is still unknown. In the present study, the expression of ANP32A was assessed in 68 CRC patients by IHC, and then the correlation of its expression with clinicopathological factors was investigated using the Allred, Klein and immune response scoring system analysis...
July 21, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28730486/phosphoproteomic-analysis-of-isolated-mitochondria-in-yeast
#9
Margaux Renvoisé, Ludovic Bonhomme, Marlène Davanture, Michel Zivy, Claire Lemaire
Mitochondria play a central role in cellular energy metabolism and cell death. Deregulation of mitochondrial functions is associated with several human pathologies (neurodegenerative diseases, neuromuscular diseases, type II diabetes, obesity, cancer). The steadily increasing number of identified mitochondrial phosphoproteins, kinases, and phosphatases in recent years suggests that reversible protein phosphorylation plays an important part in the control of mitochondrial processes. In addition, many mitochondrial phosphoproteins probably still remain to be identified, considering that 30% of proteins are expected to be phosphorylated in eukaryotes...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28730477/utilizing-the-luminex-magnetic-bead-based-suspension-array-for-rapid-multiplexed-phosphoprotein-quantification
#10
Adam Stewart, Udai Banerji
The study of protein phosphorylation is critical for the advancement of our understanding of cellular responses to external and internal stimuli. Phosphorylation, the addition of phosphate groups, most often occurs on serine, threonine, or tyrosine residues due to the action of protein kinases. This structural change causes the protein to become activated (or deactivated) and enables it in turn to initiate the phosphorylation of other proteins in a cascade, eventually causing cell-wide changes such as apoptosis, cell differentiation, and growth (among others)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28730473/expression-of-recombinant-phosphoproteins-for-signal-transduction-studies
#11
Karl W Barber, Jesse Rinehart
Complex signaling cascades are difficult to study in vitro without phosphorylated proteins. Here, we describe a technique for the routine production of recombinant phosphoproteins by directly incorporating phosphoserine as a nonstandard amino acid. This protocol utilizes an optimized phosphoserine orthogonal translation system and an engineered strain of E. coli containing no genomic amber codons. This approach has been used to generate a variety of phosphorylated proteins to understand the role of protein phosphorylation in cell signaling...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28719319/phosphoproteome-of-toxoplasma-gondii-infected-host-cells-reveals-specific-cellular-processes-predominating-in-different-phases-of-infection
#12
Cheng He, Ai-Yuan Chen, Hai-Xia Wei, Xiao-Shuang Feng, Hong-Juan Peng
The invasion of Toxoplasma gondii tachyzoites into the host cell results in extensive host cell signaling activation/deactivation that is usually regulated by the phosphorylation/dephosphorylation. To elucidate how T. gondii regulates host cell signal transduction, the comparative phosphoproteome of stable isotope labeling with amino acids in cell culture-labeled human foreskin fibroblast cells was analyzed. The cells were grouped (Light [L], Medium [M], and Heavy [H] groups) based on the labeling isotope weight and were infected with T...
July 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28714191/disordered-conformation-with-low-pii-helix-in-phosphoproteins-orchestrates-biomimetic-apatite-formation
#13
Melika Sarem, Steffen Lüdeke, Ralf Thomann, Pavel Salavei, Zhaoyong Zou, Wouter Habraken, Admir Masic, V Prasad Shastri
The interplay between noncollagenous proteins and biomineralization is widely accepted, yet the contribution of their secondary structure in mineral formation remains to be clarified. This study demonstrates a role for phosvitin, an intrinsically disordered phosphoprotein, in chick embryo skeletal development, and using circular dichroism and matrix least-squares Henderson-Hasselbalch global fitting, unravels three distinct pH-dependent secondary structures in phosvitin. By sequestering phosvitin on a biomimetic 3D insoluble cationic framework at defined pHs, access is gained to phosvitin in various conformational states...
July 17, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28710562/significant-and-unique-changes-in-phosphorylation-levels-of-four-phosphoproteins-in-two-apple-rootstock-genotypes-under-drought-stress
#14
Jing Ren, Juan Mao, Cunwu Zuo, Alejandro Calderón-Urrea, Mohammed Mujitaba Dawuda, Xin Zhao, Xinwen Li, Baihong Chen
Drought stress is a major problem around the world and there is still little molecular mechanism about how fruit crops deal with moderate drought stress. Here, the physiological and phosphoproteomic responses of drought-sensitive genotype (M26) and drought-tolerant genotype (MBB) under moderate drought stress were investigated. Our results of the physiology analysis indicated that the MBB genotype could produce more osmosis-regulating substances. Furthermore, phosphoproteins from leaves of both genotypes under moderate drought stress were analyzed using the isobaric tags for relative and absolute quantification technology...
July 14, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/28710545/comparative-muscle-proteomics-phosphoproteomics-analysis-provides-new-insight-for-the-biosafety-evaluation-of-fat-1-transgenic-cattle
#15
Xiangbo Xin, Xinfeng Liu, Xin Li, Xiangbin Ding, Shuping Yang, Congfei Jin, Guangpeng Li, Hong Guo
The biosafety of fat-1 transgenic cattle has been a focus of our studies since the first fat-1 transgenic cow was born. In this study, we used tandem mass tag labeling, TiO2 enrichment, and nanoscale liquid chromatography coupled with tandem mass spectrometry (nanol LC-MS/MS) to compare proteomic and phosphoproteomic profiling analyses of muscle between fat-1 transgenic cows and wild-type cows. A total of 1555 proteins and 900 phosphorylation sites in 159 phosphoproteins were identified in the profiling assessments, but only four differentially expressed proteins and nine differentially expressed phosphopeptides were detected in fat-1 transgenic cows relative to wild-type cows...
July 14, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28708054/the-p-gene-of-rodent-brain-adapted-measles-virus-plays-a-critical-role-in-neurovirulence
#16
Tetsuro Arai, Yuri Terao-Muto, Shotaro Uchida, Che Lin, Tomoyuki Honda, Akiko Takenaka, Fusako Ikeda, Hiroki Sato, Misako Yoneda, Chieko Kai
In rare cases, measles virus (MV) in children leads to fatal neurological complications such as primary measles encephalitis, post-acute measles encephalitis, subacute sclerosing panencephalitis and measles inclusion-body encephalitis. To investigate the pathogenesis of MV-induced encephalitis, rodent brain-adapted MV strains CAM/RB and CAMR40 were generated. These strains acquired mutations to adapt to the rodent brain during 40 passages in rat brain. However, it is still unknown which genes confer the neurovirulence of MV...
July 15, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28705056/combined-use-of-nuclear-phosphoprotein-c-myc-and-cellular-phosphoprotein-p53-for-hepatocellular-carcinoma-detection-in-high-risk-chronic-hepatitis-c-patients
#17
A M Attallah, M El-Far, M A Abdelrazek, M M Omran, A A Attallah, A A Elkhouly, H M Elkenawy, K Farid
BACKGROUND: Hepatocellular carcinoma (HCC) is a multistage process resulting from various genetic changes. We aimed to determine nuclear phosphoprotein c-Myc and cellular phosphoprotein p53 expression and to evaluate their importance in HCC diagnosis. METHODS: One hundred and twenty chronic hepatitis C (CHC) patients (60 non-HCC CHC patients and 60 HCC patients who had a single small (<5 cm) tumour) were recruited. The gene products of c-Myc and p53 were identified in liver tissues and serum samples using immunostaining, western blot and ELISA...
July 13, 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/28693611/dual-function-of-the-nuclear-export-signal-of-the-borna-disease-virus-nucleoprotein-in-nuclear-export-activity-and-binding-to-viral-phosphoprotein
#18
Mako Yanai, Madoka Sakai, Akiko Makino, Keizo Tomonaga
BACKGROUND: Borna disease virus (BoDV), which has a negative-sense, single-stranded RNA genome, causes persistent infection in the cell nucleus. The nuclear export signal (NES) of the viral nucleoprotein (N) consisting of leucine at positions 128 and 131 and isoleucine at positions 133 and 136 overlaps with one of two predicted binding sites for the viral phosphoprotein (P). A previous study demonstrated that higher expression of BoDV-P inhibits nuclear export of N; however, the function of N NES in the interaction with P remains unclear...
July 11, 2017: Virology Journal
https://www.readbyqxmd.com/read/28692105/prasugrel-in-critically-ill-patients
#19
Christian Schoergenhofer, Eva-Luise Hobl, Thomas Staudinger, Walter S Speidl, Gottfried Heinz, Jolanta Siller-Matula, Christian Zauner, Birgit Reiter, Jacek Kubica, Bernd Jilma
While prasugrel is indicated for the treatment of myocardial infarction, its effects in the most severely affected patients requiring intensive care is unknown, so that we measured the antiplatelet effects and sparse pharmacokinetics of prasugrel in critically ill patients. Twenty-three patients admitted to medical intensive care units, who were treated with 10 mg prasugrel once daily, were included in this prospective trial. Critically ill patients responded poorly to daily prasugrel treatment: adenosine diphosphate (ADP)-induced aggregation in whole blood classified 65 % (95 % confidence intervals (CI) 43-84 %) of patients as having high on treatment platelet reactivity, platelet function under high shear rates even 74 % (95 %CI 52-90 %)...
July 6, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28688217/yes-associated-protein-1-promotes-the-differentiation-and-mineralization-of-cementoblast
#20
Beining Yang, Hualing Sun, Fangfang Song, Yanru Wu, Jiawei Wang
Yes-associated protein 1 (YAP1) transcriptional coactivator is a mediator of mechanosensitive signaling. Cementum, which covers the tooth root surface, continuously senses external mechanical stimulation. Cementoblasts are responsible for the mineralization and maturation of the cementum. However, the effect of YAP1 on cementoblast differentiation remains largely unknown. In this study, we initially demonstrated that YAP1 overexpression enhanced the mineralization ability of cementoblasts. YAP1 upregulated the mRNA and protein expression of several cementogenesis markers, such as alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), osteocalcin (OCN), and dentin matrix acidic phosphoprotein 1 (DMP1)...
July 8, 2017: Journal of Cellular Physiology
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