keyword
MENU ▼
Read by QxMD icon Read
search

Confocal laser endomicroscopy

keyword
https://www.readbyqxmd.com/read/28355261/preclinical-mouse-model-to-monitor-live-muc5b-producing-conjunctival-goblet-cell-density-under-pharmacological-treatments
#1
Céline Portal, Valérie Gouyer, Frédéric Gottrand, Jean-Luc Desseyn
PURPOSE: Modification of mucous cell density and gel-forming mucin production are established hallmarks of mucosal diseases. Our aim was to develop and validate a mouse model to study live goblet cell density in pathological situations and under pharmacological treatments. METHODS: We created a reporter mouse for the gel-forming mucin gene Muc5b. Muc5b-positive goblet cells were studied in the eye conjunctiva by immunohistochemistry and probe-based confocal laser endomicroscopy (pCLE) in living mice...
2017: PloS One
https://www.readbyqxmd.com/read/28342261/diagnostic-performance-of-the-confocal-laser-endomicroscopy-of-atrophy-and-gastric-intestinal-metaplasia-a-meta-analysis
#2
Tao Bai, Lei Zhang, Stuti Sharma, Yu Dong Jiang, Jing Xia, Huan Wang, Wei Qian, Jun Song, Xiao Hua Hou
AIM: The aim of this study was to systematically evaluate its diagnostic value for atrophy and gastric intestinal metaplasia. METHODS: Extensive study search was performed in PubMed and the Cochrane Library for endomicroscopy, gastric atrophy and gastric intestinal metaplasia. Sensitivity, specificity and diagnostic odds ratio to diagnose the gastric atrophy and intestinal metaplasia were pooled. A summary receiver operating curve was documented and the zone under the curve was calculated...
March 25, 2017: Journal of Digestive Diseases
https://www.readbyqxmd.com/read/28326794/urothelial-tumors-and-dual-band-imaging-a-new-concept-in-confocal-laser-endomicroscopy
#3
Arnaud Marien, Aurélien Rock, Khaled El Maadarani, Catherine Francois, Pierre Gosset, Brigitte Mauroy, Jean-Louis Bonnal
OBJECTIVES: Confocal laser endomicroscopy (CLE) uses a low-energy laser light source to obtain microscopic histology images of bladder tissue exposed to a fluorescent dye. To evaluate the feasibility of using CLE with two fluorophores: fluorescein (FLUO) and hexylaminolevulinate (HAL) to determine histologic and cytologic bladder cancer criteria. METHODS: Patients eligible for HAL-photodynamic diagnosis-assisted transurethral resection of bladder tumor were included...
March 22, 2017: Journal of Endourology
https://www.readbyqxmd.com/read/28321928/eus-evaluation-for-pancreatic-cysts-necessity-or-overkill
#4
REVIEW
Nonthalee Pausawasdi, Thawee Ratanachu-Ek
Incidental pancreatic cysts have been gradually more recognized in clinical practice due to an increased use of transabdominal ultrasound, computed tomography (CT) and magnetic resonance imaging (MRI). These lesions consist of inflammatory cysts (pseudocysts) and pancreatic cystic neoplasms (PCNs) which have been classified as benign, pre-malignant and malignant. The diagnosis and management strategy of incidentally discovered pancreatic cysts can be challenging as the majority of them are PCNs and CT or MRI alone may not be sufficient to provide an accurate diagnosis...
March 21, 2017: Digestive Endoscopy: Official Journal of the Japan Gastroenterological Endoscopy Society
https://www.readbyqxmd.com/read/28294404/improved-classification-of-indeterminate-biliary-strictures-by-probe-based-confocal-laser-endomicroscopy-using-the-paris-criteria-following-biliary-stenting
#5
Pushpak Taunk, Satish Singh, David Lichtenstein, Virendra Joshi, Jason Gold, Ashish Sharma
BACKGROUND AND AIMS: Probe-based confocal laser endomicroscopy (pCLE) using the Miami Criteria has improved classification of indeterminate biliary strictures. However, previous biliary stenting may result in their misclassification as malignant strictures. Inflammatory criteria were added to form the Paris Classification to prevent this misclassification and reduce false positives. The aim of this study was to assess if the Paris Classification was more accurate than the Miami Classification in classifying indeterminate biliary strictures after biliary stenting...
March 11, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28294161/in-vivo-imaging-of-the-muc5b-gel-forming-mucin
#6
Céline Portal, Valérie Gouyer, Mylène Magnien, Ségolène Plet, Frédéric Gottrand, Jean-Luc Desseyn
Gel-forming mucins are macromolecules produced by goblet cells and responsible for the mucus gel formation. Changes in goblet cell density and in gel-forming mucin production have emerged as sensitive indicators for mucosal diseases. A Muc5b-GFP tagged reporter mouse was used to assess Muc5b production in mouse tissues by immunofluorescence microscopy and fluorescent activity using stereromicroscopy and probe-based confocal laser endomicroscopy. Muc5b production was followed longitudinally by recording the fluorescent activity in vagina and in embryonic lung explants under stimulation by interleukin 13...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28287510/adsc-sheet-transplantation-to-prevent-stricture-after-extended-esophageal-endoscopic-submucosal-dissection
#7
Guillaume Perrod, Laetitia Pidial, Sophie Camilleri, Alexandre Bellucci, Amaury Casanova, Thomas Viel, Bertrand Tavitian, Chirstophe Cellier, Olivier Clément, Gabriel Rahmi
In past years, the cell-sheet construct has spurred wide interest in regenerative medicine, especially for reconstructive surgery procedures. The development of diversified technologies combining adipose tissue-derived stromal cells (ADSCs) with various biomaterials has led to the construction of numerous types of tissue-engineered substitutes, such as bone, cartilage, and adipose tissues from rodent, porcine, or human ADSCs. Extended esophageal endoscopic submucosal dissection (ESD) is responsible for esophageal stricture formation...
February 10, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28286093/needle-based-confocal-laser-endomicroscopy-for-the-diagnosis-of-pancreatic-cystic-lesions-an-international-external-interobserver-and-intraobserver-study-with-videos
#8
Somashekar G Krishna, William R Brugge, John M Dewitt, Pradermchai Kongkam, Bertrand Napoleon, Carlos Robles-Medranda, Damien Tan, Samer El-Dika, Sean McCarthy, Jon Walker, Mary E Dillhoff, Andrei Manilchuk, Carl Schmidt, Benjamin Swanson, Zarine K Shah, Phil A Hart, Darwin L Conwell
BACKGROUND AND AIMS: EUS-guided nCLE characteristics of common types of PCLs have been identified; however surgical histopathology was available in a minority of subjects. We sought to assess the performance characteristics of EUS-nCLE for differentiating mucinous from non-mucinous PCLs in a larger series of subjects with a definitive diagnosis. METHODS: Six endosonographers (nCLE experience >30 cases each) blinded to all clinical data, reviewed nCLE images of PCLs from 29 subjects with surgical (n=23) or clinical (n=6) correlation...
March 9, 2017: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/28281172/needle-based-confocal-laser-endomicroscopy-for-evaluation-of-cystic-neoplasms-of-the-pancreas
#9
Abdurrahman Kadayifci, Mustafa Atar, Omer Basar, David G Forcione, William R Brugge
BACKGROUND: The accurate diagnosis of cystic neoplasms of the pancreas (CNP) with current diagnostic methods is limited. Endoscopic ultrasound (EUS)-guided needle-based confocal laser endomicroscopy (nCLE) is a new technique which can obtain images from the cyst wall during EUS-fine needle aspiration (EUS-FNA). The aim of this study was to assess the safety, feasibility, and diagnostic value of nCLE for CNP. METHODS: Patients who underwent EUS-FNA to evaluate a CNP larger than 2 cm were enrolled...
May 2017: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/28256346/past-present-and-future-of-barrett-s-oesophagus
#10
REVIEW
W K Tan, M di Pietro, R C Fitzgerald
Barrett's oesophagus is a condition which predisposes towards development of oesophageal adenocarcinoma, a highly lethal tumour which has been increasing in incidence in the Western world over the past three decades. There have been tremendous advances in the field of Barrett's oesophagus, not only in diagnostic modalities, but also in therapeutic strategies available to treat this premalignant disease. In this review, we discuss the past, present and future of Barrett's oesophagus. We describe the historical and new evolving diagnostic criteria of Barrett's oesophagus, while also comparing and contrasting the British Society of Gastroenterology guidelines, American College of Gastroenterology guidelines and International Benign Barrett's and CAncer Taskforce (BOBCAT) for Barrett's oesophagus...
February 16, 2017: European Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28249320/validation-of-needle-based-confocal-laser-endomicroscopy
#11
Lizzy Wijmans, Jouke T Annema
No abstract text is available yet for this article.
March 2017: Endoscopy
https://www.readbyqxmd.com/read/28244237/usefulness-of-confocal-laser-endomicroscopy-to-diagnose-ulcerative-colitis-associated-neoplasia
#12
Naoki Ohmiya, Noriyuki Horiguchi, Tomomitsu Tahara, Dai Yoshida, Hyuga Yamada, Mitsuo Nagasaka, Yoshihito Nakagawa, Tomoyuki Shibata, Tetsuya Tsukamoto, Makoto Kuroda
Chromoendoscopy, narrow-band imaging (NBI), and confocal laser endomicroscopy (CLE) have been introduced in ulcerative colitis (UC)-associated neoplasia surveillance. We aimed to determine the ability of CLE to differentiate among UC-associated neoplasia (differentiated type or undifferentiated type), sporadic adenoma, and circumscribed regenerative lesions. Of 665 patients with UC, we performed probe-based CLE (pCLE) on 12 patients with suspected UC-associated neoplasia in addition to magnifying chromoendoscopy with crystal violet and NBI...
February 28, 2017: Digestive Endoscopy: Official Journal of the Japan Gastroenterological Endoscopy Society
https://www.readbyqxmd.com/read/28240297/abnormal-capillary-vasodynamics-contribute-to-ictal-neurodegeneration-in-epilepsy
#13
Rocio Leal-Campanario, Luis Alarcon-Martinez, Hector Rieiro, Susana Martinez-Conde, Tugba Alarcon-Martinez, Xiuli Zhao, Jonathan LaMee, Pamela J Osborn Popp, Michael E Calhoun, Juan I Arribas, Alexander A Schlegel, Leandro L Di Stasi, Jong M Rho, Landon Inge, Jorge Otero-Millan, David M Treiman, Stephen L Macknik
Seizure-driven brain damage in epilepsy accumulates over time, especially in the hippocampus, which can lead to sclerosis, cognitive decline, and death. Excitotoxicity is the prevalent model to explain ictal neurodegeneration. Current labeling technologies cannot distinguish between excitotoxicity and hypoxia, however, because they share common molecular mechanisms. This leaves open the possibility that undetected ischemic hypoxia, due to ictal blood flow restriction, could contribute to neurodegeneration previously ascribed to excitotoxicity...
February 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28212154/optical-coherence-tomography-and-confocal-laser-endomicroscopy-in-pulmonary-diseases
#14
Lizzy Wijmans, Julia N S d'Hooghe, Peter I Bonta, Jouke T Annema
PURPOSE OF REVIEW: Current imaging techniques (X-ray, computed tomography scan, ultrasound) have limitations in the identification and quantification of pulmonary diseases, in particular, on highly detailed level. The purpose of this review is to provide an overview of the current knowledge of innovative light- and laser-based imaging techniques that might fill this gap. RECENT FINDINGS: Optical coherence tomography (OCT) and confocal laser endomicroscopy (CLE) are high-resolution imaging techniques, which, combined with bronchoscopy, provide 'near histology' detailed imaging of the airway wall, lung parenchyma, mediastinal lymph nodes, and pulmonary vasculature...
May 2017: Current Opinion in Pulmonary Medicine
https://www.readbyqxmd.com/read/28176895/advanced-endoscopic-imaging-in-gastric-neoplasia-and-preneoplasia
#15
Jonathan W J Lee, Lee Guan Lim, Khay Guan Yeoh
Conventional white light endoscopy remains the current standard in routine clinical practice for early detection of gastric cancer. However, it may not accurately diagnose preneoplastic gastric lesions. The technological advancements in the field of endoscopic imaging for gastric lesions are fast growing. This article reviews currently available advanced endoscopic imaging modalities, in particular chromoendoscopy, narrow band imaging and confocal laser endomicroscopy, and their corresponding evidence shown to improve diagnosis of preneoplastic gastric lesions...
2017: BMJ Open Gastroenterology
https://www.readbyqxmd.com/read/28146445/risk-of-cholangiocarcinoma-in-patients-with-primary-sclerosing-cholangitis-diagnosis-and-surveillance
#16
Hanno Ehlken, Roman Zenouzi, Christoph Schramm
PURPOSE OF REVIEW: Primary sclerosing cholangitis (PSC) is associated with an increased risk of hepatobiliary and extrahepatic malignancy. Particularly the risk of cholangiocarcinoma (CCA) is greatly increased. To provide potentially curative treatments for affected patients an early diagnosis of CCA is crucial. We here review the current advances with respect to CCA diagnosis and surveillance and discuss a rational approach on how to perform surveillance of CCA in PSC patients. RECENT FINDINGS: Given the shortcomings of the current modalities for the surveillance and diagnosis of CCA in PSC, recent studies have focused on novel biomarkers for CCA...
March 2017: Current Opinion in Gastroenterology
https://www.readbyqxmd.com/read/28142194/technical-feasibility-of-visualizing-myenteric-plexus-using-confocal-laser-endomicroscopy
#17
Masakuni Kobayashi, Kazuki Sumiyama, Naoki Shimojima, Naoki Shimojima, Satoshi Ieiri, Hideyuki Okano, Shunsuke Kamba, Takumi Fujimura, Seiichi Hirobe, Tatsuo Kuroda, Junko Takahashi-Fujigasaki
BACKGROUND AND AIM: In preceding studies, we identified that some fluorescent agents cloud visualization of the myenteric plexus (MP) with confocal laser endomicroscopy (CLE). In this study, we evaluated the technical feasibility of MP visualization using probe-based CLE (pCLE) with cresyl violet (CV), which has been used clinically for chromoendoscopy. METHODS: The dye affinity of CV for MP was first explored in an in vivo transgenic mouse model using neural crest derivatives labeled with GFP...
January 31, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28106950/confocal-laser-endomicroscopy-in-head-and-neck-malignancies-using-fitc-labelled-epcam-and-egf-r-antibodies-in-cell-lines-and-tumor-biopsies
#18
Anna S Englhard, Alexander Palaras, Veronika Volgger, Herbert Stepp, Brigitte Mack, Darko Libl, Olivier Gires, Christian S Betz
Intraoperative detection of residual malignant cells at tumor margins following excision of primary tumors could help improving surgery and thus patients' outcome. The feasibility of the tumor antigens epidermal growth factor receptor (EGF-R) and epithelial cell adhesion molecule (EpCAM) for antibody-dependent confocal laser scanning endomicroscopy (CLE)-mediated visualization of malignant cells was addressed. Both tumor antigens are highly and frequently expressed in the majority of carcinomas, including head and neck squamous cell carcinomas (HNSCC), and represent prognostic and therapeutic tumor target molecules...
January 20, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28104978/maximizing-the-endosonography-the-role-of-contrast-harmonics-elastography-and-confocal-endomicroscopy
#19
REVIEW
Andrada Seicean, Ofelia Mosteanu, Radu Seicean
New technologies in endoscopic ultrasound (EUS) evaluation have been developed because of the need to improve the EUS and EUS-fine needle aspiration (EUS-FNA) diagnostic rate. This paper reviews the principle, indications, main literature results, limitations and future expectations for each of the methods presented. Contrast-enhanced harmonic EUS uses a low mechanical index and highlights slow-flow vascularization. This technique is useful for differentiating solid and cystic pancreatic lesions and assessing biliary neoplasms, submucosal neoplasms and lymph nodes...
January 7, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28078907/the-diagnostic-value-of-confocal-laser-endomicroscopy-for-gastric-cancer-and-precancerous-lesions-among-asian-population-a-system-review-and-meta-analysis
#20
Hai-Ping Zhang, Sheng Yang, Wen-Hua Chen, Teng-Teng Hu, Jun Lin
OBJECTIVE: The objective of this study is to evaluate the diagnostic value of confocal laser endomicroscopy (CLE) in detection of gastric cancer (GC), gastric intraepithelial metaplasia (GIM), and gastric intraepithelial neoplasia (GIN) lesions. METHOD: PubMed, the Cochrane Library, and Wangfang databases were searched to include eligible articles about CLE in detection of gastric lesions. After study selection, quality assessment and data extraction conducted by two reviewers independently, meta-analysis was performed by Meta-Disc 1...
April 2017: Scandinavian Journal of Gastroenterology
keyword
keyword
89546
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"