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https://www.readbyqxmd.com/read/27925417/effects-of-temperature-on-multiparametric-evaluation-of-hindlimb-ischemia-with-dynamic-fluorescence-imaging
#1
Wenjuan Cai, Huizhi Guang, Chuangjian Cai, Jianwen Luo
Quantitative evaluation of hindlimb ischemia is essential for early diagnosis and therapy of peripheral arterial disease (PAD). Dynamic imaging using near-infrared (NIR) fluorophore indocyanine green (ICG) is a noninvasive and effective tool to monitor multiple vascular parameters including perfusion rate (PR), perfusion vascular density (PVD) and hemodynamics. It has been previously demonstrated that temperature changes could lead to significant variations of blood flow rate and vascular perfusion. In this paper, multiparametric evaluation of hindlimb ischemia was performed at different temperatures...
December 7, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27925210/effects-of-steam-microwave-blanching-and-different-drying-processes-on-drying-characteristics-and-quality-attributes-of-thunbergia-laurifolia-linn-leaves
#2
Traiphop Phahom, Singhanat Phoungchandang, William L Kerr
BACKGROUND: Dried Thunbergia laurifolia leaves are usually prepared using tray drying, resulting in products that have lost substantial amounts of bioactive compounds and antioxidant activity. The maturity of the raw material, blanching techniques and drying methods were investigated in order to select the best condition to produce high qualities of dried T. laurifolia leaves. RESULTS: The 1(st) stage of maturity was selected and steam-microwave blanching (SMB) for 4 min was adequate for blanching leading to the maximum recovery of bioactive compounds...
December 7, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27924586/rna-sequencing-analysis-of-salivary-extracellular-rna
#3
Blanca Majem, Feng Li, Jie Sun, David T W Wong
Salivary biomarkers for disease detection, diagnostic and prognostic assessments have become increasingly well established in recent years. In this chapter we explain the current leading technology that has been used to characterize salivary non-coding RNAs (ncRNAs) from the extracellular RNA (exRNA) fraction: HiSeq from Illumina® platform for RNA sequencing. Therefore, the chapter is divided into two main sections regarding the type of the library constructed (small and long ncRNA libraries), from saliva collection, RNA extraction and quantification to cDNA library generation and corresponding QCs...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924543/evaluating-fumonisin-gene-expression-in-fusarium-verticillioides
#4
Valeria Scala, Ivan Visentin, Francesca Cardinale
Transcript levels of key genes in a biosynthetic pathway are often taken as a proxy for metabolite production. This is the case of FUM1, encoding the first dedicated enzyme in the metabolic pathway leading to the production of the mycotoxins Fumonisins by fungal species belonging to the genus Fusarium. FUM1 expression can be quantified by different methods; here, we detail a protocol based on quantitative reverse transcriptase polymerase chain reaction (RT-qPCR), by which relative or absolute transcript abundance can be estimated in Fusaria grown in vitro or in planta...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27922661/fin-tiera-a-tool-for-assessing-technology-induced-errors
#5
Sari Palojoki, Tuuli Pajunen, Lasse Lehtonen, Kaija Saranto
BACKGROUND: Due to the complexity of healthcare processes, the potential for Health Information Systems (HIS) to cause technology-induced errors is a growing concern. Health Information Technology (HIT) errors nearly always threaten good patient care and can lead to patient harm. Instruments to allow hospitals to proactively identify areas of Electronic Health Records (EHR) safety, to set priorities and to intervene before incidents occur are currently underdeveloped. OBJECTIVES: The aim was to design a Finnish questionnaire to measure EHR users' perceptions of common EHR-related safety concerns in a specialized hospital district context through the lens of the theory of socio-technical dimensions...
December 6, 2016: Methods of Information in Medicine
https://www.readbyqxmd.com/read/27922467/lead-extraction-considerations-for-the-referring-cardiologist
#6
Ayman A Hussein, Bruce L Wilkoff
The population of patients with cardiac implantable electronic devices (CIEDs) continues to grow due to increasing indications in an aging population and breakthroughs in both the medical and the surgical care of patients with heart disease. As a result, there has been a growing need for device and lead extractions due to the growing population of patients with CIEDs and the subsequent need for system upgrades or revisions because of complications, infections, and lead advisory alerts.
January 2017: Cardiology in Review
https://www.readbyqxmd.com/read/27921150/bone-mineral-density-is-decreased-in-fibromyalgia-syndrome-a-systematic-review-and-meta-analysis
#7
Sikarin Upala, Wai Chung Yong, Anawin Sanguankeo
Previous studies have shown that fibromyalgia syndrome (FMS) is associated with low level of physical activity and exercise, which may lead to an increased risk of osteoporosis. However, studies of bone mineral density (BMD) in fibromyalgia have shown conflicting results. Thus, we conducted a systematic review and meta-analysis to better characterize the association between FMS and BMD. A comprehensive search of the databases MEDLINE and EMBASE was performed from inception through May 2016. The inclusion criterion was the observational studies' assessment of the association between fibromyalgia and bone mineral density in adult subjects...
December 5, 2016: Rheumatology International
https://www.readbyqxmd.com/read/27920245/underlying-influence-of-perception-of-management-leadership-on-patient-safety-climate-in-healthcare-organizations-a-mediation-analysis-approach
#8
Shao-Jen Weng, Seung-Hwan Kim, Chieh-Liang Wu
OBJECTIVE: We aim to draw insights on how medical staff's perception of management leadership affects safety climate with key safety related dimensions-teamwork climate, job satisfaction and working conditions. DESIGN/SETTING: A cross-sectional survey using Safety Attitude Questionnaire (SAQ) was performed in a medical center in Taichung City, Taiwan. The relationships among the dimensions in SAQ were then analyzed by structural equation modeling with a mediation analysis...
December 4, 2016: International Journal for Quality in Health Care
https://www.readbyqxmd.com/read/27919732/learning-from-heterogeneous-temporal-data-in-electronic-health-records
#9
Jing Zhao, Panagiotis Papapetrou, Lars Asker, Henrik Boström
Electronic health records contain large amounts of longitudinal data that are valuable for biomedical informatics research. The application of machine learning is a promising alternative to manual analysis of such data. However, the complex structure of the data, which includes clinical events that are unevenly distributed over time, poses a challenge for standard learning algorithms. Some approaches to modeling temporal data rely on extracting single values from time series; however, this leads to the loss of potentially valuable sequential information...
December 2, 2016: Journal of Biomedical Informatics
https://www.readbyqxmd.com/read/27919226/molar-loss-and-powder-diet-leads-to-memory-deficit-and-modifies-the-mrna-expression-of-brain-derived-neurotrophic-factor-in-the-hippocampus-of-adult-mice
#10
Yosuke Takeda, Hiroshi Oue, Shinsuke Okada, Akira Kawano, Katsunori Koretake, Makoto Michikawa, Yasumasa Akagawa, Kazuhiro Tsuga
BACKGROUND: It is known that tooth loss is known to be a risk factor for Alzheimer's disease and soft diet feeding induces memory impairment. Recent studies have shown that brain-derived neurotrophic factor (BDNF) is associated with tooth loss or soft diet in young animal model, and that BDNF expression is decreased in patients with Alzheimer's disease. However, single or combined effect of tooth loss and/or soft diet on brain function has not fully understood. Here we examined the effect of molar loss and powder diet on memory ability and the expression of BDNF mRNA in the hippocampus of adult C57BL/6J mice...
December 5, 2016: BMC Neuroscience
https://www.readbyqxmd.com/read/27918286/how-does-intentionality-of-encoding-affect-memory-for-episodic-information
#11
Michael Craig, Karla Butterworth, Jonna Nilsson, Colin J Hamilton, Peter Gallagher, Tom V Smulders
Episodic memory enables the detailed and vivid recall of past events, including target and wider contextual information. In this paper, we investigated whether/how encoding intentionality affects the retention of target and contextual episodic information from a novel experience. Healthy adults performed (1) a What-Where-When (WWW) episodic memory task involving the hiding and delayed recall of a number of items (what) in different locations (where) in temporally distinct sessions (when) and (2) unexpected tests probing memory for wider contextual information from the WWW task...
November 2016: Learning & Memory
https://www.readbyqxmd.com/read/27918253/placental-growth-factor-alone-or-in-combination-with-soluble-fms-like-tyrosine-kinase-1-as-an-aid-to-the-assessment-of-women-with-suspected-pre-eclampsia-systematic-review-and-economic-analysis
#12
Geoff K Frampton, Jeremy Jones, Micah Rose, Liz Payne
BACKGROUND: Pre-eclampsia (PE) prediction based on blood pressure, presence of protein in the urine, symptoms and laboratory test abnormalities can result in false-positive diagnoses. This may lead to unnecessary antenatal admissions and preterm delivery. Blood tests that measure placental growth factor (PlGF) or the ratio of soluble fms-like tyrosine kinase 1 (sFlt-1) to PlGF could aid prediction of PE if either were added to routine clinical assessment or used as a replacement for proteinuria testing...
November 2016: Health Technology Assessment: HTA
https://www.readbyqxmd.com/read/27917856/biogenic-synthesis-of-zinc-oxide-nanostructures-from-nigella-sativa-seed-prospective-role-as-food-packaging-material-inhibiting-broad-spectrum-quorum-sensing-and-biofilm
#13
Nasser A Al-Shabib, Fohad Mabood Husain, Faheem Ahmed, Rais Ahmad Khan, Iqbal Ahmad, Edreese Alsharaeh, Mohd Shahnawaz Khan, Afzal Hussain, Md Tabish Rehman, Mohammad Yusuf, Iftekhar Hassan, Javed Masood Khan, Ghulam Md Ashraf, Ali Mohammed Alsalme, Mohamed F Al-Ajmi, Vadim V Tarasov, Gjumrakch Aliev
Bacterial spoilage of food products is regulated by density dependent communication system called quorum sensing (QS). QS control biofilm formation in numerous food pathogens and Biofilms formed on food surfaces act as carriers of bacterial contamination leading to spoilage of food and health hazards. Agents inhibiting or interfering with bacterial QS and biofilm are gaining importance as a novel class of next-generation food preservatives/packaging material. In the present study, Zinc nanostructures were synthesised using Nigella sativa seed extract (NS-ZnNPs)...
December 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27917824/on-the-interpretation-of-reflectivity-data-from-lipid-bilayers-in-terms-of-molecular-dynamics-models
#14
Arwel V Hughes, Fillip Ciesielski, Antreas C Kalli, Luke A Clifton, Timothy R Charlton, Mark S P Sansom, John R P Webster
Neutron and X-ray reflectivity of model membranes is increasingly used as a tool for the study of membrane structures and dynamics. As the systems under study become more complex, and as long, all-atom molecular-dynamics (MD) simulations of membranes become more available, there is increasing interest in the use of MD simulations in the analysis of reflectometry data from membranes. In order to perform this, it is necessary to produce a model of the complete interface, including not only the MD-derived structure of the membrane, but also the supporting substrate and any other interfacial layers that may be present...
December 1, 2016: Acta Crystallographica. Section D, Structural Biology
https://www.readbyqxmd.com/read/27916920/dielectrically-loaded-cylindrical-resonator-based-wireless-passive-high-temperature-sensor
#15
Jijun Xiong, Guozhu Wu, Qiulin Tan, Tanyong Wei, Dezhi Wu, Sanmin Shen, Helei Dong, Wendong Zhang
The temperature sensor presented in this paper is based on a microwave dielectric resonator, which uses alumina ceramic as a substrate to survive in harsh environments. The resonant frequency of the resonator is determined by the relative permittivity of the alumina ceramic, which monotonically changes with temperature. A rectangular aperture etched on the surface of the resonator works as both an incentive and a coupling device. A broadband slot antenna fed by a coplanar waveguide is utilized as an interrogation antenna to wirelessly detect the sensor signal using a radio-frequency backscattering technique...
December 1, 2016: Sensors
https://www.readbyqxmd.com/read/27916854/fast-screening-of-antibacterial-compounds-from-fusaria
#16
Teis Esben Sondergaard, Marlene Fredborg, Ann-Maria Oppenhagen Christensen, Sofie K Damsgaard, Nikoline F Kramer, Henriette Giese, Jens Laurids Sørensen
Bio-guided screening is an important method to identify bioactive compounds from fungi. In this study we applied a fast digital time-lapse microscopic method for assessment of the antibacterial properties of secondary metabolites from the fungal genus Fusarium. Here antibacterial effects could be detected for antibiotic Y, aurofusarin, beauvericin, enniatins and fusaric acid after six hours of cultivation. The system was then used in a bio-guided screen of extracts from 14 different Fusarium species, which had been fractionated by HPLC...
November 29, 2016: Toxins
https://www.readbyqxmd.com/read/27916817/real-time-classification-of-patients-with-balance-disorders-vs-normal-subjects-using-a-low-cost-small-wireless-wearable-gait-sensor
#17
Bhargava Teja Nukala, Taro Nakano, Amanda Rodriguez, Jerry Tsay, Jerry Lopez, Tam Q Nguyen, Steven Zupancic, Donald Y C Lie
Gait analysis using wearable wireless sensors can be an economical, convenient and effective way to provide diagnostic and clinical information for various health-related issues. In this work, our custom designed low-cost wireless gait analysis sensor that contains a basic inertial measurement unit (IMU) was used to collect the gait data for four patients diagnosed with balance disorders and additionally three normal subjects, each performing the Dynamic Gait Index (DGI) tests while wearing the custom wireless gait analysis sensor (WGAS)...
November 29, 2016: Biosensors
https://www.readbyqxmd.com/read/27916692/the-effects-of-bacteria-nanoparticles-interface-on-the-antibacterial-activity-of-green-synthesized-silver-nanoparticles
#18
Aftab Ahmad, Yun Wei, Fatima Syed, Kamran Tahir, Aziz Ur Rehman, Arifullah Khan, Sadeeq Ullah, Qipeng Yuan
Neutralization of bacterial cell surface potential using nanoscale materials is an effective strategy to alter membrane permeability, cytoplasmic leakage, and ultimate cell death. In the present study, an attempt was made to prepare biogenic silver nanoparticles using biomolecules from the aqueous rhizome extract of Coptis Chinensis. The biosynthesized silver nanoparticles were surface modified with chitosan biopolymer. The prepared silver nanoparticles and chitosan modified silver nanoparticles were cubic crystalline structures (XRD) with an average particle size of 15 and 20 nm respectively (TEM, DLS)...
December 1, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27916690/acyl-homoserine-lactone-changes-the-abundance-of-proteins-and-the-levels-of-organic-acids-associated-with-stationary-phase-in-salmonella-enteritidis
#19
Felipe Alves de Almeida, Natan de Jesus Pimentel Filho, Lanna Clícia Carrijo, Cláudia Braga Pereira Bento, Maria Cristina Baracat-Pereira, Uelinton Manoel Pinto, Leandro Licursi de Oliveira, Maria Cristina Dantas Vanetti
Quorum sensing (QS) is cell-cell communication mechanism mediated by signaling molecules known as autoinducers (AIs) that lead to differential gene expression. Salmonella is unable to synthesize the AI-1 acyl homoserine lactone (AHL), but is able to recognize AHLs produced by other microorganisms through SdiA protein. Our study aimed to evaluate the influence of AI-1 on the abundance of proteins and the levels of organic acids of Salmonella Enteritidis. The presence of N-dodecyl-homoserine lactone (C12-HSL) did not interfere on the growth or the total amount of extracted proteins of Salmonella...
December 1, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27916421/protective-potential-of-averrhoa-bilimbi-fruits-in-ameliorating-the-hepatic-key-enzymes-in-streptozotocin-induced-diabetic-rats
#20
Surya B Kurup, Mini S
BACK GROUND: Diabetes is a mutifactorial disease which leads to several complications. Currently available drug regimens for management of diabetes have certain drawbacks. Need for safer and effective medicines from natural sources having potent antidiabetic activity. Averrhoa bilimbi Linn. (Oxalidaceae) is a medicinal plant and is reported to possess hypoglycemic activity. OBJECTIVE: To investigate the antidiabetic potential of Averrhoa bilimbi fruit extract in streptozotocin-induced diabetic rats...
December 1, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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