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https://www.readbyqxmd.com/read/28437706/three-phases-in-ph-dependent-heme-abstraction-from-myoglobin
#1
Sohini Mukherjee, Manjistha Mukherjee, Sabyasachi Bandyopadhyay, Abhishek Dey
The extent of heme extraction from myoglobin (Mb) by methylethyl ketone is found to be pH dependent and show three distinct phases. Parallel investigations of the protein using resonance Raman (rR) and circular dichroism (CD) across these pH regions indicate that these phases correspond to three different protonation steps in holoMb as the pH of the solution changed. The first transition occurs between pH5-6 and is due to the protonation of one of the heme propionate groups which disrupts its H-bonding with Arg 45 in the loop...
April 9, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28437663/microbial-communities-associated-with-plants-learning-from-nature-to-apply-it-in-agriculture
#2
REVIEW
Fernando Dini Andreote, Michele de Cássia Pereira E Silva
It is a new consensus that any living organism depends on its partners to strive under environmental conditions along their living period. Plants are also highly dependent on their associated microbes, which can support its development and proper protection under stressors. Along their evolution, plants learned to interact to soil microbiota, extracting their utmost capacity to provide resources for plant development and successful colonization of terrestrial systems, where the great soil biodiversity is keen on properly exert this role...
April 21, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/28437615/experimental-demonstration-of-feature-extraction-and-dimensionality-reduction-using-memristor-networks
#3
Shinhyun Choi, Jong Hoon Shin, Jihang Lee, Patrick Sheridan, Wei D Lu
Memristors have been considered as a leading candidate for a number of critical applications ranging from non-volatile memory to non-Von Neumann computing systems. Feature extraction, which aims to transform input data from a high dimensional space to a space with fewer dimensions, is an important technique widely used in machine learning and pattern recognition applications. Here, we experimentally demonstrate that memristor arrays can be used to perform principal component analysis (PCA), one of the most commonly-used feature extraction techniques, through online, unsupervised learning...
April 24, 2017: Nano Letters
https://www.readbyqxmd.com/read/28436019/antibiotic-therapy-for-pelvic-inflammatory-disease
#4
REVIEW
Ricardo F Savaris, Daniele G Fuhrich, Rui V Duarte, Sebastian Franik, Jonathan Ross
BACKGROUND: Pelvic inflammatory disease (PID) is an infection that affects 4% to 12% of young women, and is one of the most common causes of morbidity in this age group. The main intervention for acute PID is the use of broad-spectrum antibiotics which cover Chlamydia trachomatis, Neisseria gonorrhoeae, and anaerobic bacteria, administered intravenously, intramuscularly, or orally. In this review, we assessed the optimal treatment regimen for PID. OBJECTIVES: To assess the effectiveness and safety of antibiotic regimens used to treat pelvic inflammatory disease...
April 24, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28435378/differentiating-spontaneous-vertical-root-fracture-in-endodontically-treated-tooth
#5
Myung-Jin Lim, Jung-Ae Kim, Yoorina Choi, Chan-Ui Hong, Kyung-San Min
Although vertical root fracture (VRF) is mostly found in endodontically treated teeth, it also occurs spontaneously. If VRF is recognized after endodontic treatment, it is considered to be iatrogenic and can lead to legal trouble. However, legal problems can be averted if the dentist can prove that the VRF existed before endodontic treatment. This case report describes an unusual, spontaneous VRF in an endodontically treated tooth and presents a useful tip for determining whether a fracture is iatrogenic. We performed nonsurgical endodontic treatment on a mandibular first molar with irreversible pulpitis...
January 2017: European Journal of Dentistry
https://www.readbyqxmd.com/read/28435010/ribotypes-associated-with-clostridium-difficile-outbreaks-in-brazil-display-distinct-surface-protein-profiles
#6
Thais Gonçalves Ferreira, Hercules Moura, John R Barr, Regina M C Pilotto Domingues, Eliane de Oliveira Ferreira
Clostridium difficile is a spore-forming anaerobic intestinal pathogen that causes Clostridium difficile infection (CDI). C. difficile is the leading cause of toxin-mediated nosocomial antibiotic-associated diarrhea. The pathogenesis of CDI is attributed to two major virulence factors, TcdA and TcdB toxins, that cause the symptomatic infection. C. difficile also expresses a number of key proteins, including cell wall proteins (CWPs). S-layer proteins (SLPs) are CWPs that form a paracrystalline surface array that coats the surface of the bacterium...
April 20, 2017: Anaerobe
https://www.readbyqxmd.com/read/28433870/automated-detection-of-premature-delivery-using-empirical-mode-and-wavelet-packet-decomposition-techniques-with-uterine-electromyogram-signals
#7
U Rajendra Acharya, Vidya K Sudarshan, Soon Qing Rong, Zechariah Tan, Choo Min Lim, Joel Ew Koh, Sujatha Nayak, Sulatha V Bhandary
An accurate detection of preterm labor and the risk of preterm delivery before 37 weeks of gestational age is crucial to increase the chance of survival rate for both mother and the infant. Thus, the uterine contractions measured using uterine electromyogram (EMG) or electro hysterogram (EHG) need to have high sensitivity in the detection of true preterm labor signs. However, visual observation and manual interpretation of EHG signals at the time of emergency situation may lead to errors. Therefore, the employment of computer-based approaches can assist in fast and accurate detection during the emergency situation...
April 18, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28433430/complementary-effect-of-hydroquinone-and-retinoic-acid-on-corneocyte-desquamation-with-their-combination-use
#8
Kyung Ah Cheong, Tae Ryong Lee, Ai-Young Lee
BACKGROUND: Retinoic acid (RA) enhances skin-lightening capabilities of hydroquinone (HQ), at least in part, by facilitating desquamation which leads to increase penetration of HQ. The desquamation also affects skin irritation levels. The mechanism of RA-induced desquamation, however, has not been completely explored and no such data has been available for HQ uses. OBJECTIVE: To examine the role of HQ, RA, and their combination in the desquamation. METHODS: Primary cultured normal human keratinocytes, which were treated with HQ and/or RA in presence or absence of serine-specific inhibitor Kazal type5 (SPINK5)/lympho-epithelial Kazal-type-related inhibitor (LEKTI) knockdown or recombinant human SPINK5/LEKTI, and biopsied skin samples applied with HQ or RA were examined...
April 7, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28433260/future-and-potential-spending-on-health-2015-40-development-assistance-for-health-and-government-prepaid-private-and-out-of-pocket-health-spending-in-184-countries
#9
(no author information available yet)
BACKGROUND: The amount of resources, particularly prepaid resources, available for health can affect access to health care and health outcomes. Although health spending tends to increase with economic development, tremendous variation exists among health financing systems. Estimates of future spending can be beneficial for policy makers and planners, and can identify financing gaps. In this study, we estimate future gross domestic product (GDP), all-sector government spending, and health spending disaggregated by source, and we compare expected future spending to potential future spending...
April 19, 2017: Lancet
https://www.readbyqxmd.com/read/28432984/cigarette-smoke-extract-counteracts-atheroprotective-effects-of-high-laminar-flow-on-endothelial-function
#10
Sindy Giebe, Natalia Cockcroft, Katherine Hewitt, Melanie Brux, Anja Hofmann, Henning Morawietz, Coy Brunssen
Tobacco smoking and hemodynamic forces are key stimuli in the development of endothelial dysfunction and atherosclerosis. High laminar flow has an atheroprotective effect on the endothelium and leads to a reduced response of endothelial cells to cardiovascular risk factors compared to regions with disturbed or low laminar flow. We hypothesize that the atheroprotective effect of high laminar flow could delay the development of endothelial dysfunction caused by cigarette smoking. Primary human endothelial cells were stimulated with increasing dosages of aqueous cigarette smoke extract (CSEaq)...
April 7, 2017: Redox Biology
https://www.readbyqxmd.com/read/28432805/pitfalls-in-electrogram-interpretation-subcutaneous-cardioverter-defibrillator-malfunction-in-brugada-syndrome
#11
Giovanni Morani, Bruna Bolzan, Luca Tomasi, Ruggero Tomei, Corrado Vassanelli
A patient with Brugada Syndrome implanted with subcutaneous cardioverter defibrillator (S-ICD) had oversensing episodes treated with S-ICD shocks. Comparable artefacts weren't evocable with S-ICD pocket manipulation. The fluoroscopy excluded S-ICD macroscopic damage. The device extraction revealed undamaged pulse generator and connector, but the lead was inappropriately tunnelled under the sixth rib. Then the S-ICD malfunction was due to lead microscopic damage caused by lead rubbing the rib surface. This article is protected by copyright...
April 22, 2017: Pacing and Clinical Electrophysiology: PACE
https://www.readbyqxmd.com/read/28432696/systematic-review-of-self-management-interventions-for-people-with-eczema
#12
REVIEW
M J Ridd, A J L King, E Le Roux, A Waldecker, A L Huntle
Eczema is a common, long-term condition but inadequate support and information can lead to poor adherence and treatment failure. We have reviewed the international literature of interventions designed to promote self-management in adults and children with eczema. MEDLINE, MEDLINE in process, EMBASE, CINAHL and GREAT were searched from their inception to August 2016 for randomised controlled trials. Two authors independently applied eligibility criteria, assessed risk of bias for all included studies and extracted data...
April 22, 2017: British Journal of Dermatology
https://www.readbyqxmd.com/read/28432513/the-relationship-between-statins-and-breast-cancer-prognosis-varies-by-statin-type-and-exposure-time-a-meta-analysis
#13
EDITORIAL
Binliang Liu, Zongbi Yi, Xiuwen Guan, Yi-Xin Zeng, Fei Ma
PURPOSE: Breast cancer is the most common cancer in females and the leading cause of death worldwide. The effects of statins on breast cancer prognosis have long been controversial; thus, it is important to investigate the relationship between statin type, exposure time, and breast cancer prognosis. This study sought to explore the effect of statins, as well as the different effects of statin solubility and variable follow-up times, on breast cancer prognosis. METHODS: We searched the MEDLINE (via PubMed), EMBASE (via OvidSP), Cochrane Library, and ISI Web of Knowledge databases using combinations of the terms "breast neoplasms[MeSH]," "statins" or "lipid-lowering drug," "prognosis" or "survival," or "mortality" or "outcome" with no limit on the publication date...
April 21, 2017: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/28432341/detection-of-red-blood-cell-surface-antigens-by-probe-triggered-cell-collision-and-flow-retardation-in-an-autonomous-microfluidic-system
#14
Éva Sautner, Krisztián Papp, Eszter Holczer, Eszter L Tóth, Rita Ungai-Salánki, Bálint Szabó, Péter Fürjes, József Prechl
Microfluidic devices exploit combined physical, chemical and biological phenomena that could be unique in the sub-millimeter dimensions. The current goal of development of Point-of-Care (POC) medical devices is to extract the biomedical information from the blood. We examined the characteristics of blood flow in autonomous microfluidic devices with the aim to realize sensitive detection of interactions between particulate elements of the blood and the appropriately modified surfaces of the system. As a model experiment we demonstrated the fast analysis of the AB0 blood group system...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28431186/unfractionated-heparin-versus-low-molecular-weight-heparins-for-avoiding-heparin-induced-thrombocytopenia-in-postoperative-patients
#15
REVIEW
Daniela R Junqueira, Liliane M Zorzela, Edson Perini
BACKGROUND: Heparin-induced thrombocytopenia (HIT) is an adverse drug reaction presenting as a prothrombotic disorder related to antibody-mediated platelet activation. It is a paradoxical immune reaction resulting in thrombin generation in vivo, which leads to a hypercoagulable state and the potential to initiate venous or arterial thrombosis. A number of factors are thought to influence the incidence of HIT including the type and preparation of heparin (unfractionated heparin (UFH) or low molecular weight heparin (LMWH)) and the heparin-exposed patient population, with the postoperative patient population at higher risk...
April 21, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28430345/healing-response-of-rat-pulp-treated-with-an-injectable-keratin-hydrogel
#16
Lavanya Ajay Sharma, Robert M Love, Mohammad A Ali, Ajay Sharma, Soraia Macari, Avadhoot Avadhani, George J Dias
BACKGROUND: Keratin has shown promising outcomes as a biomaterial due to its inherent bioactivity, biocompatibility and regenerative effects. The effect of keratin on repair and regeneration of dental tissues has never been studied before. Current therapies to treat pulp tissues involve its replacement with inert, synthetic materials that do not have a proper biological function, leading to failure and tooth loss. This study aimed to develop a biocompatible keratin hydrogel (KH) suitable for pulp therapies...
April 20, 2017: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/28430144/a-systems-biology-approach-using-transcriptomic-data-reveals-genes-and-pathways-in-porcine-skeletal-muscle-affected-by-dietary-lysine
#17
Taiji Wang, Jean M Feugang, Mark A Crenshaw, Naresh Regmi, John R Blanton, Shengfa F Liao
Nine crossbred finishing barrows (body weight 94.4 ± 6.7 kg) randomly assigned to three dietary treatments were used to investigate the effects of dietary lysine on muscle growth related metabolic and signaling pathways. Muscle samples were collected from the longissimus dorsi of individual pigs after feeding the lysine-deficient (4.30 g/kg), lysine-adequate (7.10 g/kg), or lysine-excess (9.80 g/kg) diet for five weeks, and the total RNA was extracted afterwards. Affymetrix Porcine Gene 1.0 ST Array was used to quantify the expression levels of 19,211 genes...
April 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28428773/mutation-at-g103-of-mtbftsz-altered-their-sensitivity-to-coumarins
#18
Duggirala Sridevi, Karpagam U Sudhakar, Ragamanvitha Ananthathatmula, Rakesh P Nankar, Mukesh Doble
Coumarins are natural polyphenol lactones comprising of fused rings of benzene and α-pyrone. The current study demonstrates the inhibitory effect of coumarins with various substitutions on Mycobacterium smegmatis mc(2) 155. We also demonstrate the effect of pomegranate (Punica granatum) extract containing ellagic acid, on M. smegmatis as well as their affect on MtbFtsZ (FtsZ from Mycobacterium tuberculosis). The ellagic acid extracts from pomegranate peels inhibit mycobacteria with a MIC of 25 μM and 0.3 to 3...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28427671/ultrasound-assisted-rapid-extraction-and-kinetic-modelling-of-influential-factors-extraction-of-camptothecin-from-nothapodytes-nimmoniana-plant
#19
Dhiraj M Patil, Krishnacharya G Akamanchi
Ultrasound-assisted extraction (UAE) of commercially important natural product camptothecin (CPT) from Nothapodytes nimmoniana plant has been investigated. The influences of process factors such as electric acoustic intensity, solid to liquid ratio, duty cycle, temperature and particle size on the maximum extraction yield and kinetic mechanisms of the entire extraction process have been investigated. The kinetics results showed that increasing the intensity, duty cycle, solid to liquid ratio and decreasing the particle size lead to substantial increase in extraction yields compared to classical stirring extraction...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28427638/a-new-approach-of-probe-sonication-assisted-ionic-liquid-conversion-of-glucose-cellulose-and-biomass-into-5-hydroxymethylfurfural
#20
Ariyanti Sarwono, Zakaria Man, Nawshad Muhammad, Amir Sada Khan, Wan Suzaini Wan Hamzah, Asyraf Hanim Abdul Rahim, Zahoor Ullah, Cecilia Devi Wilfred
5-Hydroxymethylfurfural (HMF) has been identified as a promising biomass-derived platform chemical. In this study, one pot production of HMF was studied in ionic liquid (IL) under probe sonication technique. Compared with the conventional heating technique, the use of probe ultrasonic irradiation reduced the reaction time from hours to minutes. Glucose, cellulose and local bamboo, treated with ultrasonic, produced HMF in the yields of 43%, 31% and 13% respectively, within less than 10min. The influence of various parameters such as acoustic power, reaction time, catalysts and glucose loading were studied...
July 2017: Ultrasonics Sonochemistry
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