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Gil Henkin, Daniel Berard, Francis Stabile, Marjan Shayegan, Jason S Leith, Sabrina R Leslie
We present a dynamically adjustable nanofluidic platform for formatting the conformations of and visualizing the interaction kinetics between biomolecules in solution, offering new time resolution and control of the reaction processes. This platform extends Convex Lens-induced Confinement (CLiC), a technique for imaging molecules under confinement, by introducing a system for in-situ modification of the chemical environment; this system uses a deep microchannel to diffusively exchange reagents within the nanoscale imaging region, whose height is fixed by a nanopost array...
October 21, 2016: Analytical Chemistry
Yoyo Suhoyo, Gandes R Rahayu, Nani Cahyani
No abstract text is available yet for this article.
November 2016: Medical Education
Ying Wang, Andy Sischka, Volker Walhorn, Katja Tönsing, Dario Anselmetti
Fluorescent DNA dyes are broadly used in many biotechnological applications for detecting and imaging DNA in cells and gels. Their binding alters the structural and nanomechanical properties of DNA and affects the biological processes that are associated with it. Although interaction modes like intercalation and minor groove binding already have been identified, associated mechanic effects like local elongation, unwinding, and softening of the DNA often remain in question. We used magnetic tweezers to quantitatively investigate the impact of three DNA-binding dyes (YOYO-1, DAPI, and DRAQ5) in a concentration-dependent manner...
October 18, 2016: Biophysical Journal
Shirley S M Fong, Joanne W Y Chung, Yoyo T Y Cheng, Timothy T T Yam, Hsiu-Ching Chiu, Daniel Y T Fong, C Y Cheung, Lily Yuen, Esther Y T Yu, Yeung Sam Hung, Duncan J Macfarlane, Shamay S M Ng
This cross-sectional and exploratory study aimed to compare motor performance and electroencephalographic (EEG) attention levels in children with developmental coordination disorder (DCD) and those with typical development, and determine the relationship between motor performance and the real-time EEG attention level in children with DCD.Eighty-six children with DCD [DCD: n = 57; DCD and attention deficit hyperactivity disorder (ADHD): n = 29] and 99 children with typical development were recruited...
September 2016: Medicine (Baltimore)
Dipak Giri, Zi Li, Kayesh M Ashraf, Maryanne M Collinson, Daniel A Higgins
Surface wettability gradients were used to elongate and align double stranded λ-DNA. Gradients were prepared by vapor phase deposition of octyltrichlorosilane (C8-silane) and fluorinated octyltrichlorosilane (F-silane) precursors. Gradient formation was confirmed by water contact angle and ellipsometric film thickness measurements. Placement of a droplet of aqueous DNA solution on the hydrophobic end of each gradient led to spontaneous motion of the droplet toward the hydrophilic end and deposition of the DNA...
September 14, 2016: ACS Applied Materials & Interfaces
Shirley S M Fong, Shamay S M Ng, Yoyo T Y Cheng, Janet Y H Wong, Esther Y T Yu, Gary C C Chow, Yvonne T C Chak, Ivy K Y Chan, Joni Zhang, Duncan Macfarlane, Louisa M Y Chung
Objectives. To evaluate the effects of Ving Tsun (VT) martial art training on the upper extremity muscle strength and eye-hand coordination of middle-aged and older adults. Methods. This study used a nonequivalent pretest-posttest control group design. Forty-two community-dwelling healthy adults participated in the study; 24 (mean age ± SD = 68.5 ± 6.7 years) underwent VT training for 4 weeks (a supervised VT session twice a week, plus daily home practice), and 18 (mean age ± SD = 72.0 ± 6.7 years) received no VT training and acted as controls...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
B B Bruijns, R M Tiggelaar, J G E Gardeniers
In this research six cyanine fluorophores for the quantification of dsDNA in the pg-ng range, without amplification, are compared under exactly identical conditions: EvaGreen, SYBR Green, PicoGreen, AccuClear, AccuBlue NextGen and YOYO-1. The fluorescence intensity as a function of the amount of dsDNA is measured at the optimal wavelengths for excitation and emission and for each dye the limit of detection and the response linearity at low levels of dsDNA are determined. No linear range was found for SYBR Green and YOYO-1 for pg-ng quantities of dsDNA...
October 15, 2016: Analytical Biochemistry
Yoyo Hinuma, Taisuke Hatakeyama, Yu Kumagai, Lee A Burton, Hikaru Sato, Yoshinori Muraba, Soshi Iimura, Hidenori Hiramatsu, Isao Tanaka, Hideo Hosono, Fumiyasu Oba
Nitride semiconductors are attractive because they can be environmentally benign, comprised of abundant elements and possess favourable electronic properties. However, those currently commercialized are mostly limited to gallium nitride and its alloys, despite the rich composition space of nitrides. Here we report the screening of ternary zinc nitride semiconductors using first-principles calculations of electronic structure, stability and dopability. This approach identifies as-yet-unreported CaZn2N2 that has earth-abundant components, smaller carrier effective masses than gallium nitride and a tunable direct bandgap suited for light emission and harvesting...
2016: Nature Communications
Shirley S M Fong, Shamay S M Ng, Yoyo T Y Cheng, Joni Zhang, Louisa M Y Chung, Gary C C Chow, Yvonne T C Chak, Ivy K Y Chan, Duncan J Macfarlane
[Purpose] The effectiveness of a smartphone pedometer application was compared with that of a traditional pedometer for improving the physical activity and weight status of community-dwelling older adults. [Subjects and Methods] This study had a nonequivalent pretest-posttest control group design. Ninety-seven older adults (mean age ± SD, 60.1 ± 5.5 years) joined the smartphone pedometer group and underwent a 2-week walking intervention based on a smartphone pedometer application. Fifty-four older adults (mean age ± SD, 65...
May 2016: Journal of Physical Therapy Science
Gary T K Mok, Sharon S Chan, Yoyo W Y Chu, Sau Man Chan, Wilfred H S Wong, Brian H Y Chung
Normal growth is essential to a developing child. Most syndromes with dysmorphic features demonstrate recognizable patterns of disproportionate growth, thus physical measurements are an important aspect of patient evaluation. Delineating the abnormal growth pattern depends greatly on the accuracy of the normal range. However, the currently available reference data are restricted in terms of ethnicity and geographical location, hence may not be applicable to all children worldwide. In Hong Kong, reference data are limited only to the neonatal period...
August 2016: American Journal of Medical Genetics. Part A
Farhan Chowdhury, Isaac T S Li, Thuy T M Ngo, Benjamin J Leslie, Byoung Choul Kim, Joshua E Sokoloski, Elizabeth Weiland, Xuefeng Wang, Yann R Chemla, Timothy M Lohman, Taekjip Ha
Notch signaling, involved in development and tissue homeostasis, is activated at the cell-cell interface through ligand-receptor interactions. Previous studies have implicated mechanical forces in the activation of Notch receptor upon binding to its ligand. Here we aimed to determine the single molecular force required for Notch activation by developing a novel low tension gauge tether (LTGT). LTGT utilizes the low unbinding force between single-stranded DNA (ssDNA) and Escherichia coli ssDNA binding protein (SSB) (∼4 pN dissociation force at 500 nm/s pulling rate)...
June 8, 2016: Nano Letters
Maedeh Roushan, Zubair Azad, Shuang Fang Lim, Hong Wang, Robert Riehn
Intercalating fluorescent probes are widely used to visualize DNA in studies on DNA-protein interactions. Some require the presence of adenosine triphosphate (ATP). We have investigated the mechanical properties of DNA stained with the fluorescent intercalating dyes YOYO-1 and YOYO-3 as a function of ATP concentrations (up to 2 mM) by stretching single molecules in nanofluidic channels with a channel cross-section as small as roughly 100×100 nm(2). The presence of ATP reduces the length of the DNA by up to 11 %...
June 1, 2015: Mikrochimica Acta
Laura B Dickson, Maria V Sharakhova, Vladimir A Timoshevskiy, Karen L Fleming, Alex Caspary, Massamba Sylla, William C Black
Aedes aegypti, the primary vector of dengue, yellow fever and Zika flaviviruses, consists of at least two subspecies. Aedes aegypti (Aaa) is light in color, has pale scales on the first abdominal tergite, oviposits in artificial containers, and preferentially feeds on humans. Aedes aegypti formosus (Aaf), has a dark cuticle, is restricted to sub-Saharan Africa, has no pale scales on the first abdominal tergite and frequently oviposits in natural containers. Scale patterns correlate with cuticle color in East Africa but not in Senegal, West Africa where black cuticle mosquitoes display a continuum of scaling patterns and breed domestically indoors...
April 2016: PLoS Neglected Tropical Diseases
Yeh-Hsing Lao, Chun-Wei Chi, Sarah M Friedrich, Konan Peck, Tza-Huei Wang, Kam W Leong, Lin-Chi Chen
A unique interaction between the cyanine dye and negatively charged quantum dot is used to construct a signal-on biaptameric quantum dot (QD) Förster resonance energy transfer (FRET) beacon for protein detection and distinct aptamer characterization. The beacon comprises a pair of aptamers, one intercalated with the cyanine dye (YOYO-3) and the other conjugated to a negatively charged, carboxyl-QD. When the target protein is present, structural folding and sandwich association of the two aptamers take place...
May 18, 2016: ACS Applied Materials & Interfaces
Shirley S M Fong, X Guo, Yoyo T Y Cheng, Karen P Y Liu, William W N Tsang, Timothy T T Yam, Louisa M Y Chung, Duncan J Macfarlane
UNLABELLED: This study aimed to compare the effectiveness of a specific functional movement-power training (FMPT) program, a functional movement training (FMT) program and no training in the improvement of balance strategies, and neuromuscular performance in children with developmental coordination disorder (DCD). It was a randomized, single-blinded, parallel group controlled trial. METHODS: 161 children with DCD (age: 6-10 years) were randomly assigned to the FMPT, FMT, or control groups...
April 2016: Medicine (Baltimore)
Przemyslaw Szafranski, Tomasz Gambin, Avinash V Dharmadhikari, Kadir Caner Akdemir, Shalini N Jhangiani, Jennifer Schuette, Nihal Godiwala, Svetlana A Yatsenko, Jessica Sebastian, Suneeta Madan-Khetarpal, Urvashi Surti, Rosanna G Abellar, David A Bateman, Ashley L Wilson, Melinda H Markham, Jill Slamon, Fernando Santos-Simarro, María Palomares, Julián Nevado, Pablo Lapunzina, Brian Hon-Yin Chung, Wai-Lap Wong, Yoyo Wing Yiu Chu, Gary Tsz Kin Mok, Eitan Kerem, Joel Reiter, Namasivayam Ambalavanan, Scott A Anderson, David R Kelly, Joseph Shieh, Taryn C Rosenthal, Kristin Scheible, Laurie Steiner, M Anwar Iqbal, Margaret L McKinnon, Sara Jane Hamilton, Kamilla Schlade-Bartusiak, Dawn English, Glenda Hendson, Elizabeth R Roeder, Thomas S DeNapoli, Rebecca Okashah Littlejohn, Daynna J Wolff, Carol L Wagner, Alison Yeung, David Francis, Elizabeth K Fiorino, Morris Edelman, Joyce Fox, Denise A Hayes, Sandra Janssens, Elfride De Baere, Björn Menten, Anne Loccufier, Lieve Vanwalleghem, Philippe Moerman, Yves Sznajer, Amy S Lay, Jennifer L Kussmann, Jasneek Chawla, Diane J Payton, Gael E Phillips, Erwin Brosens, Dick Tibboel, Annelies de Klein, Isabelle Maystadt, Richard Fisher, Neil Sebire, Alison Male, Maya Chopra, Jason Pinner, Girvan Malcolm, Gregory Peters, Susan Arbuckle, Melissa Lees, Zoe Mead, Oliver Quarrell, Richard Sayers, Martina Owens, Charles Shaw-Smith, Janet Lioy, Eileen McKay, Nicole de Leeuw, Ilse Feenstra, Liesbeth Spruijt, Frances Elmslie, Timothy Thiruchelvam, Carlos A Bacino, Claire Langston, James R Lupski, Partha Sen, Edwina Popek, Paweł Stankiewicz
Alveolar capillary dysplasia with misalignment of pulmonary veins (ACDMPV) is a lethal lung developmental disorder caused by heterozygous point mutations or genomic deletion copy-number variants (CNVs) of FOXF1 or its upstream enhancer involving fetal lung-expressed long noncoding RNA genes LINC01081 and LINC01082. Using custom-designed array comparative genomic hybridization, Sanger sequencing, whole exome sequencing (WES), and bioinformatic analyses, we studied 22 new unrelated families (20 postnatal and two prenatal) with clinically diagnosed ACDMPV...
May 2016: Human Genetics
Hugh Fullagar, Sabrina Skorski, Rob Duffield, Tim Meyer
Elite soccer players are at risk of reduced recovery following periods of sleep disruption, particularly following late-night matches. It remains unknown whether improving sleep quality or quantity in such scenarios can improve post-match recovery. Therefore, the aim of this study was to investigate the effect of an acute sleep hygiene strategy (SHS) on physical and perceptual recovery of players following a late-night soccer match. In a randomised cross-over design, two highly-trained amateur teams (20 players) played two late-night (20:45) friendly matches against each other seven days apart...
2016: Chronobiology International
Bettina Karsten, Eneko Larumbe-Zabala, Gokhan Kandemir, Tahir Hazir, Andreas Klose, Fernando Naclerio
UNLABELLED: The objectives of this study were to examine the effects of a moderate intensity strength training on changes in critical velocity (CV), anaerobic running distance (D'), sprint performance and Yo-Yo intermittent running test (Yo-Yo IR1) performances. METHODS: two recreational soccer teams were divided in a soccer training only group (SO; n = 13) and a strength and soccer training group (ST; n = 13). Both groups were tested for values of CV, D', Yo-Yo IR1 distance and 30-m sprint time on two separate occasions (pre and post intervention)...
2016: PloS One
Genki Kobayashi, Yoyo Hinuma, Shinji Matsuoka, Akihiro Watanabe, Muhammad Iqbal, Masaaki Hirayama, Masao Yonemura, Takashi Kamiyama, Isao Tanaka, Ryoji Kanno
A variety of proton (H(+))-conducting oxides are known, including those used in electrochemical devices such as fuel cells. In contrast, pure H(-) conduction, not mixed with electron conduction, has not been demonstrated for oxide-based materials. Considering that hydride ions have an ionic size appropriate for fast transport and also a strong reducing ability suitable for high-energy storage and conversion devices, we prepared a series of K2NiF4-type oxyhydrides, La(2-x-y)Sr(x + y)LiH(1-x + y)O(3-y), in the hope of observing such H(-) conductors...
March 18, 2016: Science
H Egwuagha, O M Adegbolagun, B O Emikpe, O Odeniyi, Y Ogunremi
BACKGROUND: The current widespread distribution and use of Yoyo bitters; an herbal bitters made in Nigeria calls for an assessment of its content, efficacy and extent to which this product achieves the labelled claim of being an herbal cleanser. METHODS: The pH, analysis for trace metal and preliminary phytochemicals screening were assessed. In addition, the total phenolic acid content, antioxidant activity using DPPH inhibition and microbiological assay were evaluated using standard procedures...
June 2015: African Journal of Medicine and Medical Sciences
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