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https://www.readbyqxmd.com/read/28806601/influence-of-sublethal-doses-of-acetamiprid-and-halosulfuron-methyl-on-metabolites-of-zebra-fish-brachydanio-rerio
#1
Hong Zhang, Lijuan Zhao
Acetamiprid, a neonicotinoid pesticide, is reported to have adverse sublethal effects on non-target beneficial organisms. Halosulfuron-methyl (HM), one of the most widely used herbicides in agriculture, has high ecotoxicity to aquatic plants and animals. In this study, a GC-MS-based metabolomics approach was used to investigate the toxicity of acetamiprid and HM. The Automated Mass Spectral Deconvolution and Identification System (AMDIS) software program and the retention index method were used to identify 51 metabolites in zebra fish (Brachydanio rerio)...
August 4, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28806500/doxepin-mitigates-noise-induced-neuronal-damage-in-primary-auditory-cortex-of-mice-via-suppression-of-acid-sphingomyelinase-ceramide-pathway
#2
Yu-Ting Su, Xing-Xing Meng, Xi Zhang, Yi-Bin Guo, Hai-Jun Zhang, Yao-Ping Cheng, Xiao-Ping Xie, Yao-Ming Chang, Jun-Xiang Bao
Neuronal damage in primary auditory cortex (A1) underlies complex manifestations of noise exposure, prevention of which is critical for health maintenance. Acid sphingomyelinase (ASM) catalyzes generation of ceramide (Cer) which if over-activated mediates neuronal disorders in various diseases. Tricyclic antidepressants (TCAs), by restraining ASM/Cer, benefits multiple neuronal anomalies, so we aimed to elucidate the effect of TCA on noise induced hearing loss and auditory cortex derangement, unraveling mechanism involved...
August 14, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/28805978/obeticholic-acid-a-selective-farnesoid-x-receptor-agonist-regulates-bile-acid-homeostasis-in-sandwich-cultured-human-hepatocytes
#3
Yuanyuan Zhang, Jonathan P Jackson, Robert L St Claire, Kimberly Freeman, Kenneth R Brouwer, Jeffrey E Edwards
Farnesoid X receptor (FXR) is a master regulator of bile acid homeostasis through transcriptional regulation of genes involved in bile acid synthesis and cellular membrane transport. Impairment of bile acid efflux due to cholangiopathies results in chronic cholestasis leading to abnormal elevation of intrahepatic and systemic bile acid levels. Obeticholic acid (OCA) is a potent and selective FXR agonist that is 100-fold more potent than the endogenous ligand chenodeoxycholic acid (CDCA). The effects of OCA on genes involved in bile acid homeostasis were investigated using sandwich-cultured human hepatocytes...
August 2017: Pharmacology Research & Perspectives
https://www.readbyqxmd.com/read/28803926/differences-in-associations-of-antidepressants-and-hospitalization-due-to-hyponatremia
#4
Shermineh Farmand, Jonatan Lindh, Jan Calissendorff, Jakob Skov, Henrik Falhammar, David Nathanson, Buster Mannheimer
Background Selective serotonin reuptake inhibitors (SSRIs) and other antidepressants are important as a cause for hyponatremia. However, most studies have focused on the effect on sodium levels regardless of clinical symptoms or been too small to be able to discriminate between the effects of specific antidepressant drugs. The objective of the present study was to investigate the association between different groups of antidepressants and the risk of hospitalisation due to hyponatremia. Methods In this register based case-control study of patients in the general Swedish population we identified 14 359 individuals with a main diagnosis of hyponatremia...
August 10, 2017: American Journal of Medicine
https://www.readbyqxmd.com/read/28802408/down-regulation-of-malate-synthase-in-mycobacterium-tuberculosis-h37ra-leads-to-reduced-stress-tolerance-persistence-and-survival-in-macrophages
#5
Kumar Sachin Singh, Rishabh Sharma, Deepa Keshari, Nirbhay Singh, Sudheer Kumar Singh
Malate synthase is a condensing enzyme responsible for conversion of glyoxylate to malate in the presence of acetyl-CoA. This reaction helps in bypassing the TCA cycle reactions involving carbon loss and leads to diverting some of the carbon skeletons to gluconeogenic events while rest can continue to provide TCA cycle intermediates. Malate synthase (GlcB) is encoded by MRA_1848 of Mycobacterium tuberculosis H37Ra (Mtb-Ra). We developed a knockdown (KD) Mtb-Ra strain by down-regulating GlcB. The survival studies suggested increased susceptibility to oxidative and nitrosative stress as well as to rifampicin...
September 2017: Tuberculosis
https://www.readbyqxmd.com/read/28801776/effects-of-training-status-on-pdh-regulation-in-human-skeletal-muscle-during-exercise
#6
Anders Gudiksen, Lærke Bertholdt, Tomasz Stankiewicz, Jonas Tybirk, Peter Plomgaard, Jens Bangsbo, Henriette Pilegaard
Pyruvate dehydrogenase (PDH) is the gateway enzyme for carbohydrate-derived pyruvate feeding into the TCA cycle. PDH may play a central role in regulating substrate shifts during exercise, but the influence of training state on PDH regulation during exercise is not fully elucidated. The purpose of this study was to investigate the impact of training state on post-translational regulation of PDHa activity during submaximal and exhaustive exercise. Eight untrained and nine endurance exercise-trained healthy male subjects performed incremental exercise on a cycle ergometer: 40 min at 50% incremental peak power output (IPPO), 10 min at 65% (IPPO), followed by 80% (IPPO) until exhaustion...
August 11, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28801293/-effect-of-macrophage-inflammatory-protein-1%C3%AE-on-proliferation-and-apoptosis-of-human-tongue-squamous-cell-carcinoma-cal-27-cells-in-vitro
#7
Bo Jia, Xiao-Ling Qiu, Hong-Xing Chu, Xiang Sun, Jie Pan, Zhi-Ping Wang, Jian-Jiang Zhao
OBJECTIVE: To detect CCR5 protein expression in different human tongue squamous cell carcinoma cells and observe the effect of macrophage inflammatory protein-1β (MIP-1β) on the proliferation and apoptosis of CAL-27 cells. METHODS: Western blotting and immunofluorescence staining were used to detect the expression of the CCR5, the receptor of MIP-1β, in 3 human tongue squamous cell carcinoma cells UM-1, CAL-27, and Tca-8113. CCK-8 assay was used to assess the proliferation of CAL-27 cells stimulated with 10, 20, and 40 ng/mL MIP-1β for 12, 24, or 48 h...
August 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28800966/reconstruction-of-a-metabolic-regulatory-network-in-escherichia-coli-for-purposeful-switching-from-cell-growth-mode-to-production-mode-in-direct-gaba-fermentation-from-glucose
#8
Yuki Soma, Yuri Fujiwara, Takuya Nakagawa, Keigo Tsuruno, Taizo Hanai
γ-aminobutyric acid (GABA) is a drug and functional food additive and is used as a monomer for producing the biodegradable plastic, polyamide 4. Recently, direct GABA fermentation from glucose has been developed as an alternative to glutamate-based whole cell bioconversion. Although total productivity in fermentation is determined by the specific productivity and cell amount responsible for GABA production, the optimal metabolic state for GABA production conflicts with that for bacterial cell growth. Herein, we demonstrated metabolic state switching from the cell growth mode based on the metabolic pathways of the wild type strain to a GABA production mode based on a synthetic metabolic pathway in Escherichia coli through rewriting of the metabolic regulatory network and pathway engineering...
August 8, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28800040/effect-of-continuous-positive-airway-pressure-in-hypertensive-patients-with-obstructive-sleep-apnea-and-high-urinary-metanephrines
#9
Luisa Gilardini, Carolina Lombardi, Gabriella Redaelli, Paola Mattaliano, Paolo Fanari, Mauro Cornacchia, Massimo Scacchi, Stefania Mai, Patrizia Ferronato, Gianfranco Parati, Cecilia Invitti
OBJECTIVE: Some cases of pseudopheochromocytoma have been described among hypertensive patients with obstructive sleep apnea (OSA). This study examined whether a pathological rise of urinary metanephrines is a common feature in hypertensive OSA patients and, in such a case, whether the ventilation treatment during sleep (continuous or biphasic positive airway pressure) may normalize high metanephrines levels. METHODS: Patients with endocrine diseases, drug abuse, therapy with TCA and cardiovascular events in the previous 6 months were excluded...
August 10, 2017: Journal of Hypertension
https://www.readbyqxmd.com/read/28797234/succinyl-proteome-profiling-of-dendrobium-officinale-an-important-traditional-chinese-orchid-herb-revealed-involvement-of-succinylation-in-the-glycolysis-pathway
#10
Shangguo Feng, Kaili Jiao, Hong Guo, Mengyi Jiang, Juan Hao, Huizhong Wang, Chenjia Shen
BACKGROUND: Lysine succinylation is a ubiquitous and important protein post-translational modification in various eukaryotic and prokaryotic cells. However, its functions in Dendrobium officinale, an important traditional Chinese orchid herb with high polysaccharide contents, are largely unknown. RESULTS: In our study, LC-MS/MS was used to identify the peptides that were enriched by immune-purification with a high-efficiency succinyl-lysine antibody. In total, 314 lysine succinylation sites in 207 proteins were identified...
August 10, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28791404/betulinic-acid-increases-radiosensitization-of-oral-squamous-cell-carcinoma-through-inducing-sp1-sumoylation-and-pten-expression
#11
Dao-Ying Yuan, Zhen Meng, Kai Xu, Qiao-Feng Li, Cheng Chen, Ke-Yi Li, Bin Zhang
Radiotherapy is one of the most effective non-surgical treatments for oral squamous cell carcinoma. However, radioresistance remains a major impediment to radiotherapy. Although BetA (Betulinic acid) can induce radiosensitization, the underlying mechanism and whether it could induce radiosensitization in oral squamous cell carcinoma are not fully understood. In this study, we showed that BetA increased radiosensitization in CAL-27 and Tca-83 cells. Radiation-triggered Sp1 overexpression was responsible for radioresistance of OSCC (oral squamous cell carcinoma) cells...
August 3, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28781117/increased-coronary-tortuosity-is-associated-with-increased-left-ventricular-longitudinal-myocardial-shortening
#12
Andrew C Oehler, Jessica Minnier, Jonathan R Lindner
BACKGROUND: The mechanistic basis for tortuosity of the coronary arteries (TCA) is unclear. The aim of this study was to test the hypothesis that the relative degree of systolic longitudinal shortening of the left ventricle that deforms coaxially oriented coronary arteries is associated with TCA. METHODS: Adult subjects undergoing coronary angiography and comprehensive echocardiography within 3 months were classified dichotomously as with (n = 32) or without (n = 42) TCA defined on the basis of number and severity of coronary angles...
August 3, 2017: Journal of the American Society of Echocardiography
https://www.readbyqxmd.com/read/28780297/respiration-disruption-and-detoxification-at-the-protein-expression-levels-in-the-pacific-oyster-crassostrea-gigas-under-zinc-exposure
#13
Jie Meng, Wen-Xiong Wang, Li Li, Guofan Zhang
The Pacific oyster (Crassostrea gigas) can accumulate high levels of zinc (Zn) without obvious toxicity, but the related molecular mechanisms are largely unknown. In the present study, C. gigas were exposed to excess Zn for 9days and the differentially expressed proteins (DEPs) were examined using the isobaric tags for relative and absolute quantitation (iTRAQ) method. In total, 2667 proteins containing at least two peptides and detected in both replicates were used for proteomic analysis. Among these DEPs, 332 were up-regulated and 282 were down-regulated...
July 26, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28780039/disruption-of-5-ht2a-pdz-protein-interaction-differently-affects-the-analgesic-efficacy-of-ssri-snri-and-tca-in-the-treatment-of-traumatic-neuropathic-pain-in-rats
#14
Anne-Sophie Wattiez, Amandine Dupuis, Anne-Marie Privat, Maryse Chalus, Eric Chapuy, Youssef Aissouni, Alain Eschalier, Christine Courteix
Antidepressants remain one of the first line treatments prescribed to neuropathic pain patients despite their limited efficacy and/or their numerous side effects. More and more, pharmacotherapy for neuropathic pain has evolved towards the use of therapeutic combinations. The goal of the present study was to assess the efficacy of the combination of antidepressants - selective serotonin reuptake inhibitors and serotonin-noradrenaline reuptake inhibitors-with a peptide (TAT-2ASCV) able to disrupt the interaction between serotonin type 2A (5-HT2A) receptors and associated PDZ proteins...
August 2, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28776265/traumatic-brain-injury-alters-the-metabolism-and-facilitates-alzheimer-s-disease-in-a-murine-model
#15
Dandan Lou, Yao Du, Daochao Huang, Fang Cai, Yun Zhang, Tinyu Li, Weihui Zhou, Hongchang Gao, Weihong Song
A majority of Alzheimer's disease (AD) cases are sporadic without known cause. People who suffered from traumatic brain injury (TBI) are more likely to develop neurodegeneration and cognitive impairments. However, the role of TBI in pathophysiology of AD remains elusive. The present study intended to explore the effect of TBI on metabolism and its role in AD pathogenesis. We subjected double transgenic AD model mice APP23/PS45 to TBI. We found that TBI promoted β-secretase cleavage of amyloid β precursor protein and amyloid β protein deposition, and exuberated the cognitive impairments in AD mouse models...
August 3, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28770302/acetobacter-pasteurianus-metabolic-change-induced-by-initial-acetic-acid-to-adapt-to-acetic-acid-fermentation-conditions
#16
Yu Zheng, Renkuan Zhang, Haisong Yin, Xiaolei Bai, Yangang Chang, Menglei Xia, Min Wang
Initial acetic acid can improve the ethanol oxidation rate of acetic acid bacteria for acetic acid fermentation. In this work, Acetobacter pasteurianus was cultured in ethanol-free medium, and energy production was found to increase by 150% through glucose consumption induced by initial acetic acid. However, oxidation of ethanol, instead of glucose, became the main energy production pathway when upon culturing ethanol containing medium. Proteome assay was used to analyze the metabolism change induced by initial acetic acid, which provided insight into carbon metabolic and energy regulation of A...
August 2, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28769919/cellular-response-to-ciprofloxacin-in-low-level-quinolone-resistant-escherichia-coli
#17
Jesús Machuca, Esther Recacha, Alejandra Briales, Paula Díaz-de-Alba, Jesús Blazquez, Álvaro Pascual, José-Manuel Rodríguez-Martínez
Bactericidal activity of quinolones has been related to a combination of DNA fragmentation, reactive oxygen species (ROS) production and programmed cell death (PCD) systems. The underlying molecular systems responsible for reducing bactericidal effect during antimicrobial therapy in low-level quinolone resistance (LLQR) phenotypes need to be clarified. To do this and also define possible new antimicrobial targets, the transcriptome profile of isogenic Escherichia coli harboring quinolone resistance mechanisms in the presence of a clinical relevant concentration of ciprofloxacin was evaluated...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28768703/opposite-regulation-of-insulin-sensitivity-by-dietary-lipid-versus-carbohydrate-excess
#18
Anne-Marie Lundsgaard, Kim A Sjøberg, Louise D Høeg, Jacob Jeppesen, Andreas B Jordy, Annette K Serup, Andreas M Fritzen, Henriette Pilegaard, Lene S Myrmel, Lise Madsen, Jørgen F P Wojtaszewski, Erik A Richter, Bente Kiens
To understand the mechanisms in lipid-induced insulin resistance, a more physiologic approach is to enhance FA availability through the diet. Nine healthy men ingested two hypercaloric diets (+75 E%) for three days, enriched in unsaturated FA (78 E% fat; UNSAT) or carbohydrates (80 E% carbohydrate; CHO) as well as a eucaloric control diet (CON). Compared to CON, UNSAT reduced whole body and leg glucose disposal during a hyperinsulinemic-euglycemic clamp, while decreasing hepatic glucose production. In muscle, DAG and IMTG were increased...
August 2, 2017: Diabetes
https://www.readbyqxmd.com/read/28768149/the-biological-foundation-of-the-genetic-association-of-tomm40-with-late-onset-alzheimer-s-disease
#19
Kahli Zeitlow, Lefko Charlambous, Isaac Ng, Sonal Gagrani, Mirta Mihovilovic, Shuhong Luo, Daniel L Rock, Ann Saunders, Allen D Roses, W Kirby Gottschalk
A variable-length poly-T variant in intron 6 of the TOMM40 gene, rs10524523, is associated with risk and age-of-onset of sporadic (late-onset) Alzheimer's disease. In Caucasians, the three predominant alleles at this locus are Short (S), Long (L) or Very long (VL). On an APOE ε3/3 background, the S/VL and VL/VL genotypes are more protective than S/S. The '523 poly-T has regulatory properties, in that the VL poly-T results in higher expression than the S poly-T in luciferase expression systems. The aim of the current work was to identify effects on cellular bioenergetics of increased TOM40 protein expression...
July 30, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28767134/the-differential-impact-of-amino-acids-on-oxphos-system-activity-following-carbohydrate-starvation-in-arabidopsis-cell-suspensions
#20
João Henrique F Cavalcanti, Carla G S Quinhones, Peter Schertl, Danielle S Brito, Holger Eubel, Tatjana Hildebrandt, Adriano Nunes-Nesi, Hans-Peter Braun, Wagner L Araújo
Plant respiration mostly depends on the activity of glycolysis and the oxidation of organic acids in the tricarboxylic acid (TCA) cycle to synthesize ATP. However, during stress situations plant cells also use amino acids as alternative substrates to donate electrons via the ETF/ETFQO complex to the mitochondrial electron transport chain (mETC). Given to this, here we investigated changes of the oxidative phosphorylation (OXPHOS) system in Arabidopsis thaliana cell culture under carbohydrate starvation supplied with a range of amino acids...
August 2, 2017: Physiologia Plantarum
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