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https://www.readbyqxmd.com/read/27902722/canonical-wnt-signaling-drives-tumor-like-lesions-from-sox2-positive-precursors-of-the-murine-olfactory-epithelium
#1
Nils W Engel, Julia E Neumann, Julia Ahlfeld, Annika K Wefers, Daniel J Merk, Jasmin Ohli, Ulrich Schüller
Canonical Wnt signaling is known to promote proliferation of olfactory stem cells. In order to investigate the effects of a constitutive activation of Wnt signaling in Sox2-positive precursor cells of the olfactory epithelium, we used transgenic mice that allowed an inducible deletion of exon 3 of the Ctnnb1 gene, which is responsible for the phosphorylation and degradation of Ctnnb1 protein. After induction of aberrant Wnt activation by Ctnnb1 deletion at embryonic day 14, such mice developed tumor-like lesions in upper parts of the nasal cavity...
2016: PloS One
https://www.readbyqxmd.com/read/27902671/prehospital-transport-for-pediatric-trauma-a-comparison-of-private-transport-and-emergency-medical-services
#2
Yea-Chyi Lin, York Tien Lee, Jasmine Xun Yi Feng, Li Wei Chiang, Shireen Anne Nah
OBJECTIVES: We describe the demographics of pediatric patients with trauma transferred using private transport (PT) versus emergency medical services (EMS) and evaluate the potential impact on their treatment and outcome. METHODS: We accessed data from our national trauma registry, a prospectively collected database. Data were extracted on all patients with trauma admitted to our institution between January 2011 and June 2013, with injury severity score (ISS) higher than 8...
November 29, 2016: Pediatric Emergency Care
https://www.readbyqxmd.com/read/27902183/lactobacillus-ixorae-sp-nov-isolated-from-a-flower-west-indian-jasmine
#3
Sujitra Techo, Mika Miyashita, Chiyo Shibata, Naoto Tanaka, Preeyarach Wisetkhan, Wonnop Visessanguan, Somboon Tanasupawat
A Gram-stain positive, lactic acid bacterium strain Ru20-1T was isolated from a flower (West-Indian Jasmine) collected from Kalasin province, Thailand. Polyphasic approaches were used to determine the taxonomic position of this strain. Studies of morphological and biochemical characteristics revealed that strain Ru20-1T belonged to the genus Lactobacillus. The strain was heterofermentative, non-spore forming and rod-shaped. It produced DL-lactic acid. Based on 16S rRNA gene sequences similarity, this strain was closely related to Lactobacillus lindneri LMG 14528T (96...
October 11, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27901042/transplanted-photoreceptor-precursors-transfer-proteins-to-host-photoreceptors-by-a-mechanism-of-cytoplasmic-fusion
#4
Mandeep S Singh, Jasmin Balmer, Alun R Barnard, Sher A Aslam, Daniela Moralli, Catherine M Green, Alona Barnea-Cramer, Isabel Duncan, Robert E MacLaren
Photoreceptor transplantation is a potential future treatment for blindness caused by retinal degeneration. Photoreceptor transplantation restores visual responses in end-stage retinal degeneration, but has also been assessed in non-degenerate retinas. In the latter scenario, subretinal transplantation places donor cells beneath an intact host outer nuclear layer (ONL) containing host photoreceptors. Here we show that host cells are labelled with the donor marker through cytoplasmic transfer-94±4.1% of apparently well-integrated donor cells containing both donor and host markers...
November 30, 2016: Nature Communications
https://www.readbyqxmd.com/read/27900519/early-dynamic-imaging-in-68-ga-psma-11-pet-ct-allows-discrimination-of-urinary-bladder-activity-and-prostate-cancer-lesions
#5
Christian Uprimny, Alexander Stephan Kroiss, Clemens Decristoforo, Josef Fritz, Boris Warwitz, Lorenza Scarpa, Llanos Geraldo Roig, Dorota Kendler, Elisabeth von Guggenberg, Jasmin Bektic, Wolfgang Horninger, Irene Johanna Virgolini
PURPOSE: PET/CT with (68)Ga-labelled prostate-specific membrane antigen (PSMA)-ligands has been proven to establish a promising imaging modality in the work-up of prostate cancer (PC) patients with biochemical relapse. Despite a high overall detection rate, the visualisation of local recurrence may be hampered by high physiologic tracer accumulation in the urinary bladder on whole body imaging, usually starting 60 min after injection. This study sought to verify whether early dynamic (68)Ga-PSMA-11 (HBED-CC)PET/CT can differentiate pathologic PC-related tracer uptake from physiologic tracer accumulation in the urinary bladder...
November 29, 2016: European Journal of Nuclear Medicine and Molecular Imaging
https://www.readbyqxmd.com/read/27899761/rehabilitation-augments-hematoma-clearance-and-attenuates-oxidative-injury-and-ion-dyshomeostasis-after-brain-hemorrhage
#6
Michael R Williamson, Kristen Dietrich, Mark J Hackett, Sally Caine, Colby A Nadeau, Jasmine R Aziz, Helen Nichol, Phyllis G Paterson, Frederick Colbourne
BACKGROUND AND PURPOSE: We assessed the elemental and biochemical effects of rehabilitation after intracerebral hemorrhage, with emphasis on iron-mediated oxidative stress, using a novel multimodal biospectroscopic imaging approach. METHODS: Collagenase-induced striatal hemorrhage was produced in rats that were randomized to enriched rehabilitation or control intervention starting on day 7. Animals were euthanized on day 14 or 21, a period of ongoing cell death...
November 29, 2016: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/27897385/reconstruction-of-biological-pathways-and-metabolic-networks-from-in-silico-labeled-metabolites
#7
Noushin Hadadi, Jasmin Hafner, Keng Cher Soh, Vassily Hatzimanikatis
Reaction atom mappings track the positional changes of all of the atoms between the substrates and the products as they undergo the biochemical transformation. However, information on atom transitions in the context of metabolic pathways is not widely available in the literature. The understanding of metabolic pathways at the atomic level is of great importance as it can deconvolute the overlapping catabolic/anabolic pathways resulting in the observed metabolic phenotype. The automated identification of atom transitions within a metabolic network is a very challenging task since the degree of complexity of metabolic networks dramatically increases when we transit from metabolite-level studies to atom-level studies...
November 29, 2016: Biotechnology Journal
https://www.readbyqxmd.com/read/27896813/transcriptional-and-post-transcriptional-regulation-of-histone-variant-h2a-z-during-sea-urchin-development
#8
Mihai Hajdu, Jasmine Calle, Andrea Puno, Aminat Haruna, César Arenas-Mena
Histone variant H2A.Z promotes chromatin accessibility at transcriptional regulatory elements and is developmentally regulated in metazoans. We characterize the transcriptional and post-transcriptional regulation of H2A.Z in the purple sea urchin Strongylocentrotus purpuratus. H2A.Z depletion by antisense translation-blocking morpholino oligonucleotides during early development causes developmental collapse, in agreement with its previously demonstrated general role in transcriptional multipotency. During H2A...
November 29, 2016: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/27895686/successful-treatment-of-a-67-year-old-woman-with-urethral-adenocarcinoma-with-the-use-of-external-beam-radiotherapy-and-image-guided-adaptive-interstitial-brachytherapy
#9
Jasmin Mujkanovic, Kari Tanderup, Mads Agerbæk, Ulla Bisgaard, Søren Høyer, Jacob Christian Lindegaard, Lars Fokdal
Primary urethral cancer (PUC) is a very rare disease. This case report illustrates a successful treatment approach of a 67-year-old woman with a urethral adenocarcinoma selected for an organ preserving treatment with external beam radiotherapy (EBRT) and interstitial brachytherapy (BT) boost, using the GEC-ESTRO target concept originally designed for locally advanced cervical cancer (LACC). Treatment included EBRT with 45 Gy in 25 fractions followed by image guided adaptive interstitial BT (IGABT) with a pulsed-dose-rate (PDR) BT boost with 30 Gy in 50 hourly pulses...
October 2016: Journal of Contemporary Brachytherapy
https://www.readbyqxmd.com/read/27893988/endothelin-signaling-in-bone
#10
REVIEW
Jasmin Kristianto, Michael G Johnson, Rafia Afzal, Robert D Blank
The endothelin (ET) system includes 3 small peptide hormones and a pair of G protein-coupled receptors. All 3 ETs are secreted as biologically inert precursors that must be activated by proteolytic cleavage after secretion. This reaction can be catalyzed by a pair of specific, membrane-bound extracellular ET converting enzymes or by nonspecific tissue proteases. The ET1/EDNRA axis is essential in development, with knockout mice for either displaying a similar phenotype featuring multiple defects of the craniofacial skeleton and cardiac outflow tract...
November 11, 2016: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/27892921/an-essential-role-for-bacterial-nitric-oxide-synthase-in-staphylococcus-aureus-electron-transfer-and-colonization
#11
Traci L Kinkel, Smirla Ramos-Montañez, Jasmine M Pando, Daniel V Tadeo, Erin N Strom, Stephen J Libby, Ferric C Fang
Nitric oxide (NO(•)) is a ubiquitous molecular mediator in biology. Many signalling actions of NO(•) generated by mammalian NO(•) synthase (NOS) result from targeting of the haem moiety of soluble guanylate cyclase. Some pathogenic and environmental bacteria also produce a NOS that is evolutionary related to the mammalian enzymes, but a bacterial haem-containing receptor for endogenous enzymatically generated NO(•) has not been identified previously. Here, we show that NOS of the human pathogen Staphylococcus aureus, in concert with an NO(•)-metabolizing flavohaemoprotein, regulates electron transfer by targeting haem-containing cytochrome oxidases under microaerobic conditions to maintain membrane bioenergetics...
November 28, 2016: Nature Microbiology
https://www.readbyqxmd.com/read/27891289/nor-bni-antagonism-of-kappa-opioid-agonist-induced-reinstatement-of-ethanol-seeking-behavior
#12
Erin Harshberger, Emily A Gilson, Kelli Gillett, Jasmine H Stone, Laila El Amrani, Glenn R Valdez
Recent work suggests that the dynorphin (DYN)/kappa opioid receptor (KOR) system may be a key mediator in the behavioral effects of alcohol. The objective of the present study was to examine the ability of the KOR antagonist norbinaltorphimine (nor-BNI) to attenuate relapse to ethanol seeking due to priming injections of the KOR agonist U50,488 at time points consistent with KOR selectivity. Male Wistar rats were trained to self-administer a 10% ethanol solution, and then responding was extinguished. Following extinction, rats were injected with U50,488 (0...
2016: Journal of Addiction
https://www.readbyqxmd.com/read/27891115/methanotrophy-under-versatile-conditions-in-the-water-column-of-the-ferruginous-meromictic-lake-la-cruz-spain
#13
Kirsten Oswald, Corinne Jegge, Jana Tischer, Jasmine Berg, Andreas Brand, María R Miracle, Xavier Soria, Eduardo Vicente, Moritz F Lehmann, Jakob Zopfi, Carsten J Schubert
Lakes represent a considerable natural source of methane to the atmosphere compared to their small global surface area. Methanotrophs in sediments and in the water column largely control methane fluxes from these systems, yet the diversity, electron accepting capacity, and nutrient requirements of these microorganisms have only been partially identified. Here, we investigated the role of electron acceptors alternative to oxygen and sulfate in microbial methane oxidation at the oxycline and in anoxic waters of the ferruginous meromictic Lake La Cruz, Spain...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27891097/molecular-characterization-of-aquaporin-1-and-aquaporin-3-from-the-gills-of-the-african-lungfish-protopterus-annectens-and-changes-in-their-branchial-mrna-expression-levels-and-protein-abundance-during-three-phases-of-aestivation
#14
You R Chng, Jasmine L Y Ong, Biyun Ching, Xiu L Chen, Kum C Hiong, Wai P Wong, Shit F Chew, Siew H Lam, Yuen K Ip
African lungfishes can undergo long periods of aestivation on land during drought. During aestivation, lungfishes are confronted with desiccation and dehydration, and their gills become non-functional and covered with a thick layer of dried mucus. Aquaporins (Aqps) are a superfamily of integral membrane proteins which generally facilitate the permeation of water through plasma membranes. This study aimed to obtain the complete cDNA coding sequences of aqp1 and aqp3 from the gills of Protopterus annectens, and to determine their branchial mRNA and protein expression levels during the induction, maintenance and arousal phases of aestivation...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27886076/pathogens-inactivated-by-low-energy-electron-irradiation-maintain-antigenic-properties-and-induce-protective-immune-responses
#15
Jasmin Fertey, Lea Bayer, Thomas Grunwald, Alexandra Pohl, Jana Beckmann, Gaby Gotzmann, Javier Portillo Casado, Jessy Schönfelder, Frank-Holm Rögner, Christiane Wetzel, Martin Thoma, Susanne M Bailer, Ekkehard Hiller, Steffen Rupp, Sebastian Ulbert
Inactivated vaccines are commonly produced by incubating pathogens with chemicals such as formaldehyde or β-propiolactone. This is a time-consuming process, the inactivation efficiency displays high variability and extensive downstream procedures are often required. Moreover, application of chemicals alters the antigenic components of the viruses or bacteria, resulting in reduced antibody specificity and therefore stimulation of a less effective immune response. An alternative method for inactivation of pathogens is ionizing radiation...
November 23, 2016: Viruses
https://www.readbyqxmd.com/read/27885886/arial-fibrillation-in-patients-with-chronic-lymphocytic-leukemia-cll
#16
Tait D Shanafelt, Sameer A Parikh, Peter A Noseworthy, Valentin Goede, Kari G Chaffee, Jasmin Bahlo, Timothy G Call, Susan M Schwager, Wei Ding, Barbara Eichhorst, Kirsten Fischer, Jose F Leis, Asher Alban Chanan-Khan, Michael Hallek, Susan L Slager, Neil E Kay
Although preliminary data suggests that ibrutinib may increase risk of atrial fibrillation (AF), the incidence of AF in a general cohort of chronic lymphocytic leukemia (CLL) patients is unknown. We evaluated the prevalence of AF at CLL diagnosis and incidence of AF during follow-up in 2444 patients with newly diagnosed CLL. A prior history of AF was present at CLL diagnosis in 148 (6.1%). Among the 2292 patients without history of AF, 139 (6.1%) developed incident AF during follow-up (incidence approximately 1%/year)...
November 25, 2016: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/27884468/corrigendum-to-endosulfan-pentachlorobenzene-and-short-chain-chlorinated-paraffins-in-background-soils-from-western-europe-environ-pollut-196-2015-21-28
#17
Anne Karine Halse, Martin Schlabach, Jasmin K Schuster, Kevin C Jones, Eiliv Steinnes, Knut Breivik
No abstract text is available yet for this article.
November 21, 2016: Environmental Pollution
https://www.readbyqxmd.com/read/27884109/cloning-functional-expression-and-characterization-of-a-bifunctional-3-hydroxybutanal-dehydrogenase-reductase-involved-in-acetone-metabolism-by-desulfococcus-biacutus
#18
Jasmin Frey, Hendrik Rusche, Bernhard Schink, David Schleheck
BACKGROUND: The strictly anaerobic, sulfate-reducing bacterium Desulfococcus biacutus can utilize acetone as sole carbon and energy source for growth. Whereas in aerobic and nitrate-reducing bacteria acetone is activated by carboxylation with CO2 to acetoacetate, D. biacutus involves CO as a cosubstrate for acetone activation through a different, so far unknown pathway. Proteomic studies indicated that, among others, a predicted medium-chain dehydrogenase/reductase (MDR) superfamily, zinc-dependent alcohol dehydrogenase (locus tag DebiaDRAFT_04514) is specifically and highly produced during growth with acetone...
November 25, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/27884015/joseph-fr%C3%A3-d%C3%A3-ric-beno%C3%A3-t-charri%C3%A3-re-how-to-explain-his-success-as-one-of-the-most-famous-surgical-instrument-makers-regarding-his-life-from-childhood-to-death
#19
Jasmin Katrin Badawi
INTRODUCTION: We use the name 'Charrière' every day as a unit of measurement. This article explains his success as one of the most famous instrument makers who ever lived. MATERIALS AND METHODS: A review of the literature was completed using PubMed. Additionally, material of historical sources like the museum of Bulle was used. RESULTS: Joseph-Frédéric-Benoît Charrière was born in Switzerland in 1803. At the age of 13, he moved to Paris and learned the profession of a cutler...
November 25, 2016: Urologia Internationalis
https://www.readbyqxmd.com/read/27881144/comparison-of-treatment-response-remission-rate-and-drug-adherence-in-polyarticular-juvenile-idiopathic-arthritis-patients-treated-with-etanercept-adalimumab-or-tocilizumab
#20
Gerd Horneff, Ariane Klein, Jens Klotsche, Kirsten Minden, Hans-Iko Huppertz, Frank Weller-Heinemann, Jasmin Kuemmerle-Deschner, Johannes-Peter Haas, Anton Hospach
BACKGROUND: Treatment response, remission rates and compliance in patients with polyarticular juvenile idiopathic arthritis (polyJIA) treated with adalimumab, etanercept, or tocilizumab were analyzed in clinical practice. METHODS: Data collected in the German BIKER registry were analyzed in patients with polyJIA who started treatment with approved biologics, adalimumab, etanercept or tocilizumab, from 2011 to 2015. Baseline patient characteristics, treatment response, safety and drug survival were compared...
November 24, 2016: Arthritis Research & Therapy
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