keyword
MENU ▼
Read by QxMD icon Read
search

leprae

keyword
https://www.readbyqxmd.com/read/28088473/endothelium-adhesion-molecules-icam-1-icam-2-vcam-1-and-vla-4-expression-in-leprosy
#1
Juarez de Sousa, Tinara Leila Sousa Aarão, Jorge Rodrigues de Sousa, Kelly Emi Hirai, Luciana Mota Silva, Leonidas Braga Dias, Francisca Regina Oliveira Carneiro, Hellen Thais Fuzii, Juarez Aantonio Simoes Quaresma
Leprosy triggers a complex relationship between the pathogen and host immune response. Endothelium plays an important role in this immune response by directly influencing cell migration to infected tissues. The objective of this work is to investigate the possible role of endothelium in M. leprae infection, correlating the characteristics of endothelial markers with the expression pattern of cytokines. Thirty-six skin biopsy samples were cut into 5-μm thick sections and stained with hematoxylin-eosin and Ziehl-Neelsen for morphological analysis and then submitted to immunohistochemical analysis using monoclonal antibodies against ICAM-1, ICAM-2, VCAM-1, and VLA-4...
January 11, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28059670/mycobacterial-hsp65-antigen-upregulates-the-cellular-immune-response-of-healthy-individuals-compared-to-tuberculosis-patients
#2
Pryscilla Fanini Wowk, Luís Henrique Franco, Denise Morais Fonseca, Marina Oliveira Paula, Élcio Dos Santos Oliveira Vianna, Ana Paula Wendling, Valéria Maria Augusto, Silvana Maria Elói-Santos, Andréa Teixeira-Carvalho, Flávia Dias Coelho Silva, Solange Alves Vinhas, Olindo Assis Martins-Filho, Moisés Palaci, Célio Lopes Silva, Vânia Luiza Deperon Bonato
Previously we showed that 65-kDa Mycobacterium leprae heat shock protein (Hsp65) is a target for the development of a tuberculosis vaccine. Here we evaluated peripheral blood mononuclear cells (PBMC) from healthy individuals or tuberculosis patients stimulated with two forms of Hsp65 antigen, recombinant DNA that encodes Hsp65 (DNA-HSP65) or recombinant Hsp65 protein (rHsp65) in attempting to mimic a prophylactic or therapeutic study in vitro, respectively. Proliferation and cytokine-producing CD4(+) or CD8(+) cell were assessed by flow cytometry...
January 6, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28056107/autophagy-is-an-innate-mechanism-associated-with-leprosy-polarization
#3
Bruno Jorge de Andrade Silva, Mayara Garcia de Mattos Barbosa, Priscila Ribeiro Andrade, Helen Ferreira, José Augusto da Costa Nery, Suzana Côrte-Real, Gilberto Marcelo Sperandio da Silva, Patricia Sammarco Rosa, Mario Fabri, Euzenir Nunes Sarno, Roberta Olmo Pinheiro
Leprosy is a chronic infectious disease that may present different clinical forms according to the immune response of the host. Levels of IFN-γ are significantly raised in paucibacillary tuberculoid (T-lep) when compared with multibacillary lepromatous (L-lep) patients. IFN-γ primes macrophages for inflammatory activation and induces the autophagy antimicrobial mechanism. The involvement of autophagy in the immune response against Mycobacterium leprae remains unexplored. Here, we demonstrated by different autophagic assays that LC3-positive autophagosomes were predominantly observed in T-lep when compared with L-lep lesions and skin-derived macrophages...
January 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28038394/increased-il-35-producing-tregs-and-cd19-il-35-cells-are-associated-with-disease-progression-in-leprosy-patients
#4
Mohd Tarique, Chaman Saini, Raza Ali Naqvi, Neena Khanna, D N Rao
BACKGROUND: The clinical forms of leprosy consist of a spectrum that reflects the host's immune response to the M. leprae; it provides an ideal model to study the host pathogen interaction and immunological dysregulation in humans. IL-10 and TGF-β producing Tregs are high in leprosy patients and responsible for immune suppression and M. leprae specific T cells anergy. In leprosy, involvement of IL-35 producing Tregs and Bregs remain unstudied. OBJECTIVE: To study the role of IL-35 producing Tregs and Bregs in the human leprosy...
December 27, 2016: Cytokine
https://www.readbyqxmd.com/read/28027305/evaluation-of-polymerase-chain-reaction-pcr-with-slit-skin-smear-examination-sss-to-confirm-clinical-diagnosis-of-leprosy-in-eastern-nepal
#5
Shraddha Siwakoti, Keshav Rai, Narayan Raj Bhattarai, Sudha Agarwal, Basudha Khanal
BACKGROUND: Detection of Mycobacterium leprae in slit skin smear (SSS) is a gold standard technique for the leprosy diagnosis. Over recent years, molecular diagnosis by using PCR has been increasingly used as an alternative for its diagnosis due to its higher sensitivity. This study was carried out for comparative evaluation of PCR and SSS microscopy in a cohort of new leprosy cases diagnosed in B. P. Koirala Institute of health Sciences, Dharan, Nepal. METHODOLOGY/PRINCIPAL FINDINGS: In this prospective crossectional study, 50 new clinically diagnosed cases of leprosy were included...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28001221/number-of-leprosy-reactions-during-treatment-clinical-correlations-and-laboratory-diagnosis
#6
Douglas Eulálio Antunes, Gabriela Porto Ferreira, Mariana Vitorino Candeiro Nicchio, Sergio Araujo, Ana Carolina Rodrigues da Cunha, Rafaela Resende Gomes, Adeilson Viera da Costa, Isabela Maria Bernardes Goulart
INTRODUCTION: The occurrence of leprosy reactions, a common event during treatment, may be mostly related to the action of multidrug therapy on Mycobacterium leprae. The clinical and laboratory monitoring of patients with reactions is important, since collecting data that assists in predicting the risk of reactions may help to prevent disability. METHODS: This was a sectional study, in order to correlate clinical and laboratory diagnosis with the number of reactions during treatment...
November 2016: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/27976721/a-large-scale-genome-wide-association-and-meta-analysis-identified-four-novel-susceptibility-loci-for-leprosy
#7
Zhenzhen Wang, Yonghu Sun, Xi'an Fu, Gongqi Yu, Chuan Wang, Fangfang Bao, Zhenhua Yue, Jianke Li, Lele Sun, Astrid Irwanto, Yongxiang Yu, Mingfei Chen, Zihao Mi, Honglei Wang, Pengcheng Huai, Yi Li, Tiantian Du, Wenjun Yu, Yang Xia, Hailu Xiao, Jiabao You, Jinghui Li, Qing Yang, Na Wang, Panpan Shang, Guiye Niu, Xiaojun Chi, Xiuhuan Wang, Jing Cao, Xiujun Cheng, Hong Liu, Jianjun Liu, Furen Zhang
Leprosy, a chronic infectious disease, results from the uncultivable pathogen Mycobacterium leprae (M. leprae), and usually progresses to peripheral neuropathy and permanent progressive deformity if not treated. Previously published genetic studies have identified 18 gene/loci significantly associated with leprosy at the genome-wide significant level. However as a complex disease, only a small proportion of leprosy risk could be explained by those gene/loci. To further identify more susceptibility gene/loci, we hereby performed a three-stage GWAS comprising 8,156 leprosy patients and 15,610 controls of Chinese ancestry...
December 15, 2016: Nature Communications
https://www.readbyqxmd.com/read/27941127/leprosy-in-a-patient-infected-with-hiv
#8
Clare M Galtrey, Hamid Modarres, Zane Jaunmuktane, Sebastian Brandner, Alexander M Rossor, Diana Nj Lockwood, Mary M Reilly, Hadi Manji, Fred Schon
A 60-year-old Nigerian man, who had lived in Europe for 30 years but had returned home frequently, presented with right frontalis muscle weakness and right ulnar nerve palsy, without skin lesions. Neurophysiology showed a generalised neuropathy with demyelinating features. Blood tests were positive for HIV, with a normal CD4 count. There was nerve thickening both clinically and on MRI. Nerve biopsy showed chronic endoneuritis and perineuritis (indicating leprosy) without visible mycobacteria. His neuropathy continued to deteriorate (lepra reaction) before starting treatment with WHO multidrug therapy, highly active antiretroviral therapy and corticosteroids...
December 9, 2016: Practical Neurology
https://www.readbyqxmd.com/read/27927694/th9-cytokines-response-and-its-possible-implications-in-the-immunopathogenesis-of-leprosy
#9
Jorge Rodrigues de Sousa, Carla Pagliari, Dandara Simone Maia de Almeida, Luiz Fernando Lima Barros, Francisca Regina Oliveira Carneiro, Leonidas Braga Dias, Tinara Leila de Souza Aarão, Juarez Antonio Simões Quaresma
AIMS: Leprosy is an infectious-contagious disease whose clinical evolution depends on the interaction of the infectious agent with the immune response of the host, leading to a clinical spectrum that ranges from lepromatous leprosy (susceptibility, LL) to tuberculoid leprosy (resistance, TT). The immune response profile will depend on the pattern of cytokine production and on the activity of macrophages during infection. Classically, the clinical evolution of leprosy has been associated with Th1/Th2 cytokine profiles, but the role of new cytokine profiles such as T helper 9 (Th9) remains to be elucidated...
December 7, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27925336/non-exponential-growth-of-mycobacterium-leprae-thai-53-strain-cultured-in-vitro
#10
Kazunobu Amako, Ken-Ichiro Iida, Mitsumasa Saito, Yoshitosi Ogura, Tetsuya Hayashi, Shin-Ichi Yoshida
To determine why cultivation of Mycobacterium leprae in vitro has not been successful, we attempted to culture this bacterium in media supplemented with a variety of biological materials. We observed a slight increase in the number of cells in medium supplemented with human blood plasma and an extract of nude mouse tissue after more than 3 months of cultivation at 30°C. To ascertain whether this increase was real growth, we analyzed the growth by droplet digital PCR. The analysis had the following two results: the slow increase in the copy number of cell-associated DNA and the release of a large amount of DNA into the culture medium from bacterial cells during cultivation...
December 7, 2016: Microbiology and Immunology
https://www.readbyqxmd.com/read/27919284/can-baseline-ml-flow-test-results-predict-leprosy-reactions-an-investigation-in-a-cohort-of-patients-enrolled-in-the-uniform-multidrug-therapy-clinical-trial-for-leprosy-patients-in-brazil
#11
Emerith Mayra Hungria, Regiane Morillas Oliveira, Gerson Oliveira Penna, Lúcio Cartaxo Aderaldo, Maria Araci de Andrade Pontes, Rossilene Cruz, Heitor de Sá Gonçalves, Maria Lúcia Fernandes Penna, Ligia Regina Franco Sansigolo Kerr, Mariane Martins de Araújo Stefani, Samira Bührer-Sékula
BACKGROUND: The predictive value of the serology to detection of IgM against the Mycobacterium leprae-derived phenolic glycolipid-I/PGL-I to identify leprosy patients who are at higher risk of developing reactions remains controversial. Whether baseline results of the ML Flow test can predict leprosy reactions was investigated among a cohort of patients enrolled in The Clinical Trial for Uniform Multidrug Therapy for Leprosy Patients in Brazil (U-MDT/CT-BR). METHODS: This was a descriptive study focusing on the main clinical manifestations of leprosy patients enrolled in the U-MDT/CT-BR from March 2007 to February 2012 at two Brazilian leprosy reference centers...
December 6, 2016: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/27891346/evaluation-of-fluorescent-staining-for-diagnosis-of-leprosy-and-its-impact-on-grading-of-the-disease-comparison-with-conventional-staining
#12
Deepa Sowkur Anandarama Adiga, Surekha B Hippargi, Gururaja Rao, Debarshi Saha, Bheemrao Ramling Yelikar, Mahesh Karigoudar
INTRODUCTION: Leprosy is a chronic infectious disease caused by Mycobacterium leprae (M. leprae). Histopathological examination of skin lesion is the gold standard for diagnosis. We evaluated the possible role of fluorescent microscopy in this direction which is increasingly used for rapid screening. AIM: To compare the efficacy of auramine rhodamine stain with Ziehl-Neelsen and modified Fite-faraco staining in diagnosing M. leprae in tissue sections. STUDY DESIGN: Experimental, cross-sectional and retrospective study conducted for 4 years...
October 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27888674/qpcr-detection-of-mycobacterium-leprae-in-biopsies-and-slit-skin-smear-of-different-leprosy-clinical-forms
#13
Michelle de Campos Soriani Azevedo, Natália Mortari Ramuno, Luciana Raquel Vincenzi Fachin, Mônica Tassa, Patrícia Sammarco Rosa, Andrea de Faria Fernandes Belone, Suzana Madeira Diório, Cleverson Teixeira Soares, Gustavo Pompermaier Garlet, Ana Paula Favaro Trombone
Leprosy, whose etiological agent is Mycobacterium leprae, is a chronic infectious disease that mainly affects the skin and peripheral nervous system. The diagnosis of leprosy is based on clinical evaluation, whereas histopathological analysis and bacilloscopy are complementary diagnostic tools. Quantitative PCR (qPCR), a current useful tool for diagnosis of infectious diseases, has been used to detect several pathogens including Mycobacterium leprae. The validation of this technique in a robust set of samples comprising the different clinical forms of leprosy is still necessary...
November 24, 2016: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27876828/common-variants-in-the-parl-and-pink1-genes-increase-the-risk-to-leprosy-in-han-chinese-from-south-china
#14
Dong Wang, Deng-Feng Zhang, Jia-Qi Feng, Guo-Dong Li, Xiao-An Li, Xiu-Feng Yu, Heng Long, Yu-Ye Li, Yong-Gang Yao
Leprosy is a chronic infectious and neurological disease caused by Mycobacterium leprae, an unculturable pathogen with massive genomic decay and dependence on host metabolism. We hypothesized that mitochondrial genes PARL and PINK1 would confer risk to leprosy. Thirteen tag SNPs of PARL and PINK1 were analyzed in 3620 individuals with or without leprosy from China. We also sequenced the entire exons of PARL, PINK1 and PARK2 in 80 patients with a family history of leprosy by using the next generation sequencing technology (NGS)...
November 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27864529/subversion-of-schwann-cell-glucose-metabolism-by-mycobacterium-leprae
#15
Rychelle Clayde Affonso Medeiros, Karina do Carmo de Vasconcelos Girardi, Fernanda Karlla Luz Cardoso, Bruno de Siqueira Mietto, Thiago Gomes de Toledo Pinto, Lilian Sales Gomez, Luciana Silva Rodrigues, Mariana Gandini, Julio Jablonski Amaral, Sérgio Luiz Gomes Antunes, Suzana Corte-Real, Patricia Sammarco Rosa, Maria Cristina Vidal Pessolani, José Augusto da Costa Nery, Euzenir Nunes Sarno, Leonardo Ribeiro Batista-Silva, Mauro Sola-Penna, Marcus Fernandes de Oliveira, Milton Ozório Moraes, Flavio Alves Lara
No abstract text is available yet for this article.
November 18, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27856491/excavating-the-surface-associated-and-secretory-proteome-of-mycobacterium-leprae-for-identifying-vaccines-and-diagnostic-markers-relevant-immunodominant-epitopes
#16
Aarti Rana, Shweta Thakur, Nupur Bhardwaj, Devender Kumar, Yusuf Akhter
For centuries, Mycobacterium leprae, etiological agent of leprosy, has been afflicting mankind regardless of extensive use of live-attenuated vaccines and antibiotics. Surface-associated and secretory proteins (SASPs) are attractive targets against bacteria. We have integrated biological knowledge with computational approaches and present a proteome-wide identification of SASPs. We also performed computational assignment of immunodominant epitopes as coordinates of prospective antigenic candidates in most important class of SASPs, the outer membrane proteins (OMPs)...
December 2016: Pathogens and Disease
https://www.readbyqxmd.com/read/27854511/a-negative-feedback-loop-between-autophagy-and-immune-responses-in-mycobacterium-leprae-infection
#17
Yuelong Ma, Li Zhang, Jie Lu, Tiejun Shui, Jia Chen, Jun Yang, Joanna Yuan, Yeqiang Liu, Degang Yang
The obligate intracellular bacterium Mycobacterium leprae is the causative agent of leprosy and primarily infects macrophages, leading to irreversible nerve damage and deformities. So far, the underlying reasons allowing M. leprae to persist and propagate in macrophages, despite the presence of cellular immunity, are still a mystery. Here, we investigated the role of autophagy, a cellular process that degrades cytosolic materials and intracellular pathogens, in M. leprae infection. We found that live M. leprae infection of macrophages resulted in significantly elevated autophagy level...
January 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/27846605/red-squirrels-in-the-british-isles-are-infected-with-leprosy-bacilli
#18
Charlotte Avanzi, Jorge Del-Pozo, Andrej Benjak, Karen Stevenson, Victor R Simpson, Philippe Busso, Joyce McLuckie, Chloé Loiseau, Colin Lawton, Janne Schoening, Darren J Shaw, Jérémie Piton, Lucio Vera-Cabrera, Jesùs S Velarde-Felix, Fergal McDermott, Stephen V Gordon, Stewart T Cole, Anna L Meredith
Leprosy, caused by infection with Mycobacterium leprae or the recently discovered Mycobacterium lepromatosis, was once endemic in humans in the British Isles. Red squirrels in Great Britain (Sciurus vulgaris) have increasingly been observed with leprosy-like lesions on the head and limbs. Using genomics, histopathology, and serology, we found M. lepromatosis in squirrels from England, Ireland, and Scotland, and M. leprae in squirrels from Brownsea Island, England. Infection was detected in overtly diseased and seemingly healthy animals...
November 11, 2016: Science
https://www.readbyqxmd.com/read/27795222/-immunological-evaluation-during-treatment-of-a-case-of-borderline-lepromatous-leprosy
#19
Blanca María Morfín-Maciel, María Carmen Jiménez Martínez
BACKGROUND: Leprosy is a chronic granulomatous infection that affects skin and peripheral nerves. Its prevalence has declined, but is still observed mainly in poor rural areas. CASE REPORT: A male city dweller with photophobia and chronic dermatosis in the face: nodular and erythematous lesions, pustules, keratitis and entropion, partial eyebrows loss, and edema on eyelids, chin, and nose bridge. The rest of the body had no lesion or lymphadenopathy. Biopsy revealed Langhans giant cell proliferation in the superficial dermis without epidermal atrophy...
October 2016: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
https://www.readbyqxmd.com/read/27764137/role-of-teffector-memory-cells-tbx21-gene-expression-and-t-cell-homing-receptor-on-type-1-reaction-in-borderline-lepromatous-leprosy-patients
#20
Luciana Nahar Dos Santos, Pedro Henrique Lopes da Silva, Iris Maria Peixoto Alvim, José Augusto da Costa Nery, Flávio Alves Lara, Euzenir Nunes Sarno, Danuza Esquenazi
In spite of hyporesponsivity to Mycobacterium leprae, borderline lepromatous (BL) patients show clinical and immunological instability, and undergo frequent acute inflammatory episodes such as type 1 reaction (T1R), which may cause nerve damages. This work focused on the participation of T cell subsets from blood and skin at T1R onset. We observed a significantly increased ex vivo frequency of both effector and memory CD4+ and CD8+ T cells in T1R group. Besides, ex vivo frequency of T cell homing receptor, the Cutaneous Leukocyte-associated Antigen (CLA) was significantly increased in T cells from T1R patients...
2016: PloS One
keyword
keyword
89331
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"