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Morphological assessment

Jacqueline Cloos, Jeffrey R Harris, Jeroen J W M Janssen, Angele Kelder, F Huang, Gerrit Sijm, Maike Vonk, Alexander N Snel, Jennifer R Scheick, Willemijn J Scholten, Jannemieke Carbaat-Ham, Dennis Veldhuizen, Diana Hanekamp, Yvonne J M Oussoren-Brockhoff, Gertjan J L Kaspers, Gerrit J Schuurhuis, A Kate Sasser, Gert Ossenkoppele
Response criteria in acute myeloid leukemia (AML) has recently been re-established, with morphologic examination utilized to determine whether patients have achieved complete remission (CR). Approximately half of the adult patients who entered CR will relapse within 12 months due to the outgrowth of residual AML cells in the bone marrow. The quantitation of these remaining leukemia cells, known as minimal or measurable residual disease (MRD), can be a robust biomarker for the prediction of these relapses. Moreover, retrospective analysis of several studies has shown that the presence of MRD in the bone marrow of AML patients correlates with poor survival...
March 5, 2018: Journal of Visualized Experiments: JoVE
Milena L Pachowsky, Arnd Kleyer, Lisa Wagner, Andreas Langenbach, David Simon, Rolf Janka, Matthias May, Goetz H Welsch
Objective A minimally invasive treatment of osteoporotic and nonosteoporotic thoracic and lumbar spine fractures is cement augmentation (kyphoplasty). Little is known about the impact on adjacent intervertebral discs. A quantitative magnetic resonance imaging (MRI) approach in addition to morphological MRI is desirable to evaluate changes in the intervertebral disc. Our study aims to evaluate the feasibility of T2 mapping for the detection of subtle changes in the intervertebral discs in spines after kyphoplasty...
March 1, 2018: Cartilage
Meng-Tsan Tsai, Jia-Wei Zhang, Kuo-Chen Wei, Chih-Kuang Yeh, Hao-Li Liu
Focused ultrasound (FUS) in combination with microbubbles temporally and locally increases the permeability of the blood-brain barrier (BBB) for facilitating drug delivery. However, the temporary effects of FUS on the brain microstructure and microcirculation need to be addressed. We used label-free optical coherence tomography (OCT) and OCT angiography (OCTA) to investigate the morphological and microcirculation changes in mouse brains due to FUS exposure at different power levels. Additionally, the recovery progress of the induced effects was studied...
February 1, 2018: Biomedical Optics Express
Le Kang, Mingsan Miao
Objective: To observe the intervention effect of total flavonoid of ilex pubesceus on animal models of cerebral ischemic tolerance. Methods: A rat model of global-focal cerebral ischemic tolerance was established by blocking bilateral common carotid artery blood flow and occluding left middle cerebral artery using thread-occlusion method. After the first operation, the Ginaton group and large-dosage, medium-dosage and small-dosage groups of total flavonoid of ilex pubesceus were given intragastric administration of corresponding drugs...
December 2017: Saudi Journal of Biological Sciences
Szu-Yuan Wu, Nam-Nhut Phan, Shih-Hsin Ho, Yu-Heng Lai, Tsai Chih-Hung, Yang Chung-Han, Kuan-Lun Li, Hang-Gan Yu, Jung-Chieh Wang, Pung-Ling Huang, Yen-Chang Lin
CONTEXT: Arsenic poisoning commonly occurs through exposure to water contaminated with arsenic and causes long-term symptoms. Of all the arsenic derivatives, arsenite is the one of the most toxic compounds. However, the toxicity of arsenite during developmental stages is still unclear. OBJECTIVE: In this study, we performed a metabolomic analysis of arsenite responses in embryonic zebrafish. MATERIALS AND METHODS: Embryonic zebrafish were used as an animal model in this study...
March 15, 2018: Toxicology Letters
Juan C Santos, Rebecca D Tarvin, Lauren A O'Connell, David C Blackburn, Luis A Coloma
Symbionts (e.g., endoparasites and commensals) play an integral role in their host's ecology, yet in many cases their diversity is likely underestimated. Although endoparasites are traditionally characterized using morphology, sequences of conserved genes, and shotgun metagenomics, host transcriptomes constitute an underused resource to identify these organisms' diversity. By isolating non-host transcripts from host transcriptomes, individual host tissues can now simultaneously reveal their endoparasite species richness (i...
March 15, 2018: Molecular Phylogenetics and Evolution
Paola Spitalieri, Rosa V Talarico, Silvia Caioli, Michela Murdocca, Annalucia Serafino, Marco Girasole, Simone Dinarelli, Giovanni Longo, Sabina Pucci, Annalisa Botta, Giuseppe Novelli, Cristina Zona, Ruggiero Mango, Federica Sangiuolo
Myotonic Dystrophy type 1 (DM1) is a multisystemic disease, autosomal dominant, caused by a CTG repeat expansion in DMPK gene. We assessed the appropriateness of patient-specific induced pluripotent stem cell-derived cardiomyocytes (CMs) as a model to recapitulate some aspects of the pathogenetic mechanism involving cardiac manifestations in DM1 patients. Once obtained in vitro, CMs have been characterized for their morphology and their functionality. CMs DM1 show intranuclear foci and transcript markers abnormally spliced respect to WT ones, as well as several irregularities in nuclear morphology, probably caused by an unbalanced lamin A/C ratio...
March 15, 2018: Journal of Molecular and Cellular Cardiology
Dandan Han, Lanying Wang, Yanping Luo
Actinomycetes are an important group of gram-positive bacteria that play an essential role in the rhizosphere ecosystem. The confrontation culture and Oxford cup method were used to evaluate the antagonistic activities of strains, which were isolated from the rhizosphere soil of Mikania micrantha. The two isolates were identified using morphological and physiological tests combined with 16S rRNA-based molecular analysis, respectively. The type I polyketone synthase (PKS-I) was amplified. The constituents of fermentation metabolites were analyzed by gas chromatography mass spectrometry...
March 2018: Microbiological Research
Niloofar Vaghefi, Julie R Kikkert, Frank S Hay, Gavriela D Carver, Lori B Koenick, Melvin D Bolton, Linda E Hanson, Gary A Secor, Sarah J Pethybridge
The taxonomy and evolutionary species boundaries in a global collection of Cercospora isolates from Beta vulgaris was investigated based on sequences of six loci. Species boundaries were assessed using concatenated multi-locus phylogenies, Generalized Mixed Yule Coalescent (GMYC), Poisson Tree Processes (PTP), and Bayes factor delimitation (BFD) framework. Cercospora beticola was confirmed as the primary cause of Cercospora leaf spot (CLS) on B. vulgaris. Cercospora apii, C. cf. flagellaris, Cercospora sp...
April 2018: Fungal Biology
X Ren, T Zhao, J Wang, H H Zhu, H Jiang, J S Jia, S M Yang, B Jiang, D B Wang, X J Huang, Q Jiang
Objective: To explore outcomes in adult with de novo acute myeloid leukemia (AML) received IA10 (10 mg/m(2) d1-3 idarubicin plus cytarabine 100 mg/m(2) d1-7) regimen as induction chemotherapy. Methods: From January 2008 to February 2016, data of consecutive newly-diagnosed AML (non-M(3)) adults treated with IA10 who achieved morphologic leukemia-free state (MLFS) but not accepted allogeneic hematopoietic stem cell transplantation (allo-HSCT) were assessed retrospectively. Results: A total of 198 patients were included in this study with 96 (48...
January 14, 2018: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
Chayul Lee, Sanghyun Park, Ikhbayar Ayush, Kyungyun Cho, Sung Soo Kim, Insug Kang, Wonchae Choe, Yoon-Seong Kim, Kyung-Sik Yoon
Using MCF7 breast cancer cells, we tested the anticancer activity of metabolites from 130 strains of myxobacteria newly isolated in South Korea. Of these, three strains whose metabolites had high anticancer activity and low cell toxicity were selected and identified by fruiting body morphology, cell morphology, and 16S rRNA sequence. Strains KYC4030 and KYC4048 were determined to be Myxococcus fulvus , whereas strain KYC4081 was identified as Corallococcus coralloides . We found that metabolites of M. fulvus KYC4048 demonstrated no toxicity in normal cells but specifically induced cancer cell death by suppressing the expression of WNT2B...
March 16, 2018: Journal of Microbiology and Biotechnology
Michael R Deschenes, Shuhan Li, Matthew A Adan, Jane J Oh, Hailey C Ramsey
BACKGROUND: This project aimed to determine the adaptability of the neuromuscular system to the stimuli of exercise training, and aging. METHODS: Young adult, and aged male rats were randomly assigned to either exercise training, or sedentary control groups. Exercise training featured an 8 week program of treadmill running. At the end of the intervention period, neuromuscular function was quantified with ex vivo stimulation procedures on isolated soleus muscles...
March 14, 2018: Experimental Gerontology
Jaya D Chidambaram, Namperumalsamy V Prajna, Srikanthi Palepu, Shruti Lanjewar, Manisha Shah, Shanmugam Elakkiya, David Macleod, Prajna Lalitha, Matthew J Burton
PURPOSE: To determine cellular features of fungal (FK), Acanthamoeba (AK) and bacterial keratitis (BK) using HRT3 in vivo confocal microscopy (IVCM). DESIGN: Prospective observational cross-sectional study. METHODS: Eligible participants were adults with microbiologically-positive FK, AK or BK, of size ≥3mm attending Aravind Eye Hospital from Feb 2012 to Feb 2013. EXCLUSION CRITERIA: descemetocele, perforation. At presentation, IVCM imaging was performed, then corneal scrapes obtained for culture/light microscopy...
March 14, 2018: American Journal of Ophthalmology
Martha Kim, Ho-Kyung Choung, Kyoung Min Lee, Sohee Oh, Seok Hwan Kim
PURPOSE: To delineate longitudinal changes in the optic nerve head (ONH) and peripapillary structure during myopia progression in childhood using spectral-domain (SD) OCT and to explore the factors associated with myopic ONH and peripapillary changes. DESIGN: Prospective cohort study. PARTICIPANTS: Twenty-three healthy children with myopia (46 eyes). METHODS: The participants underwent fundus photography, SD OCT, and axial length (AXL) measurements every 6 months for 2 years...
March 14, 2018: Ophthalmology
Michael E Zilm, Le Yu, William A Hines, Mei Wei
A detailed magnetization study, along with an assessment of the cellular proliferation, has been carried out on transition-metal-doped hydroxyapatite (HA), Ca10-x Mx (PO4 )6 (OH)2 , where M = Mn, Co, and Fe. In particular, a series of MnHA powder samples with an x value of 0.04 ≤ x ≤ 1.21, one CoHA (x = 0.48) and one FeHA sample (x = 1.06) were synthesized using a wet chemical method along with an ion-exchange procedure. Characterization by transmission electron microscope (TEM), energy-dispersive X-ray spectroscopy (EDXS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) indicated that the substitution of M elements does not change the morphology and crystalline structure of pure HA that showing a single phased HA nano-rod...
June 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Boris Aleksovski, Violeta Neceva, Viktorija Vujović, Nensi Manusheva, Vladimir Rendevski, Antoni Novotni, Ana Filipce, Anastazija Spasovska, Aspazija Sofijanova, Vasko Aleksovski, Icko Gjorgoski
INTRODUCTION: Adverse effects with bleeding disorders are often associated with the administration of SSRI in depression, although the exact mechanisms remain contradicting. This study is aimed at detecting and exploring the mechanisms of SSRI-induced changes in platelet reactivity in non-responding patients with Recurrent Depressive Disorder (RDD) and life-long exposure to antidepressants. MATERIALS AND METHODS: Thirty-one patients and thirty-one healthy controls were included in the study...
March 10, 2018: Thrombosis Research
Qi Wang, Wei Li, Ruina Liu, Kai Zhang, Haohui Zhang, Shuanliang Fan, Zhenyuan Wang
Human and non-human identification of unknown skeletal remains is of great importance in forensic and anthropologic contexts. However, the traditional morphological methods for bone species identification are subjective or time-consuming. Here, we utilized Fourier transform infrared (FTIR) spectroscopy and chemometric methods to determinate the spectral variances between human and non-human (i.e., pig, goat, and cow) bones. To simulate real forensic situations as much as possible, fresh, boiled, and decomposed bones were included in this study...
March 16, 2018: International Journal of Legal Medicine
Michael Brines, Daniel A Culver, Maryam Ferdousi, Martijn R Tannemaat, Monique van Velzen, Albert Dahan, Rayaz A Malik
Small fiber neuropathy (SFN) is a common feature of many inflammatory diseases, often presenting with pain and disability. SFN is diagnosed using symptoms, thermal threshold testing, and intra-epidermal nerve fiber quantification. Corneal confocal microscopy (CCM) is an ophthalmic imaging technique which non-invasively quantifies corneal nerve fiber (CNF) density, branch density and length, and has comparable diagnostic and superior ability to identify nerve regeneration compared to skin biopsy. CNF size (width and area) depends upon the number of fibers within each nerve, as well as pathology (e...
March 16, 2018: Scientific Reports
Monica Montefalcone, Giada De Falco, Ettore Nepote, Martina Canessa, Marco Bertolino, Giorgio Bavestrello, Carla Morri, Carlo Nike Bianchi
Marine caves are unique and vulnerable habitats exhibiting high biodiversity and heterogeneity, but threatened by multiple global and local disturbances. Marine caves, although widely distributed along the Mediterranean coast, suffer for the lack of quantitative data on their structure and function, which hinder their conservation status assessment. Thanks to the availability of a nearly 30-year-long series of data (1986-2013), we evaluated ecosystem change in the Bergeggi marine cave (Ligurian Sea, NW Mediterranean), a cave with a complex shape and high habitat heterogeneity...
February 21, 2018: Marine Environmental Research
K L Colman, M C L Janssen, K E Stull, R R van Rijn, R J Oostra, H H de Boer, A E van der Merwe
Sex estimation techniques are frequently applied in forensic anthropological analyses of unidentified human skeletal remains. While morphological sex estimation methods are able to endure population differences, the classification accuracy of metric sex estimation methods are population-specific. No metric sex estimation method currently exists for the Dutch population. The purpose of this study is to create Dutch population specific sex estimation formulae by means of osteometric analyses of the proximal femur...
December 23, 2017: Forensic Science International
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