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https://www.readbyqxmd.com/read/29793141/new-contributions-to-gruberia-lanceolata-gruber-1884-kahl-1932-based-on-analyses-of-multiple-populations-and-genes-ciliophora-heterotrichea-gruberiidae
#1
Xiangrui Chen, Shahed Uddin Ahmed Shazib, Ji Hye Kim, Min Seok Kim, Mann Kyoon Shin
Gruberia Kahl, 1932 is a species-poor genus comprising only seven named species. Most of these species have not been reinvestigated since the original reports. In the present work, we investigated the taxonomy and phylogeny of Gruberia lanceolata (Gruber, 1884) Kahl, 1932 based on analyses of morphology and multiple gene sequences from four South Korean populations. This species is mainly characterized by a well-developed peristome region, segmented paroral membrane, and moniliform macronucleus. Some morphological features were not stable among the four populations investigated, such as body shape and size, cell color, and the ratio of oral length to body length...
May 9, 2018: European Journal of Protistology
https://www.readbyqxmd.com/read/29792527/enteric-neural-stem-cells-expressing-insulin-like-growth-factor-1-a-novel-cellular-therapy-for-hirschsprung-s-disease-in-mouse-model
#2
Wei Liu, Lijuan Zhang, Rongde Wu
Transplanting enteric neural stem cells (ENSCs) is an innovative approach for replacing enteric neurons in Hirschsprung's disease (HSCR). However, posttransplantation cell survival and differentiation limit efficacy. We aimed to investigate whether transplantation of ENSCs engineered with insulin-like growth factor 1 (IGF-1) could improve survival and differentiation of the engrafted cells and promote functional recovery of the aganglionic colon. ENSCs were isolated from the intestine of neonatal mice and genetically modified to express IGF-1...
May 24, 2018: DNA and Cell Biology
https://www.readbyqxmd.com/read/29792158/landraces-of-snake-melon-an-ancient-middle-eastern-crop-reveal-extensive-morphological-and-dna-diversity-for-potential-genetic-improvement
#3
Samer Omari, Yuri Kamenir, Jennifer I C Benichou, Sarah Pariente, Hanan Sela, Rafael Perl-Treves
BACKGROUND: Snake melon (Cucumis melo var. flexuosus, "Faqqous") is a traditional and ancient vegetable in the Mediterranean area. A collection of landraces from 42 grower fields in Israel and Palestinian territories was grown and characterized in a "Common Garden" rain-fed experiment, at the morphological-horticultural and molecular level using seq-DArT markers. RESULTS: The different landraces ("populations") showed extensive variation in morphology and quantitative traits such as yield and femaleness, and clustered into four horticultural varieties...
May 23, 2018: BMC Genetics
https://www.readbyqxmd.com/read/29791233/association-of-a-chromosome-locus-9p21-3-cdkn2b-as1-variant-rs4977574-with-hypertension-the-tamrisk-study
#4
Tarja Kunnas, Jaakko Piesanen, Seppo T Nikkari
AIMS: Chromosome locus 9p21.3 CDKN2B antisense RNA 1 (CDKN2B-AS1) has been found to contain multiple genetic markers for coronary artery disease (CAD) by genome-wide association studies (GWAS). Of these, the association of variants rs4977574, rs10757274, and rs2383206 with hypertension was studied in the Tampere adult population cardiovascular risk study (TAMRISK). MATERIALS AND METHODS: A Finnish cohort of 336 subjects diagnosed with hypertension and 444 controls was analyzed...
May 2018: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/29791141/fluorogenic-probing-of-membrane-protein-trafficking
#5
Chenge Li, Aurélien Mourton, Marie-Aude Plamont, Vanessa Rodrigues, Isabelle Aujard, Michel Volovitch, Thomas Le Saux, Franck Perez, Sophie Vriz, Ludovic Jullien, Alain Joliot, Arnaud Gautier
Methods to differentially label cell-surface and intracellular membrane proteins are indispensable for understanding their function and the regulation of their trafficking. We present an efficient strategy for the rapid and selective fluorescent labeling of membrane proteins based on the chemical-genetic fluorescent marker FAST (fluorescence-activating and absorption-shifting tag). Cell-surface FAST-tagged proteins could be selectively and rapidly labeled using fluorogenic membrane-impermeant 4-hydroxybenzylidene rhodanine (HBR) analogs...
May 23, 2018: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29790972/isolation-and-biological-characterization-of-mesenchymal-stem-cells-from-goose-dermis
#6
Jingjing Wang, Xulun Wu, Yanjie Zheng, Hebao Wen, Hongda Ji, Yuhua Zhao, Weijun Guan
The skin is a natural target of stem cell research because of its large size and easy accessibility. Cutaneous mesenchymal stem cells have shown to be a promising source of various adult stem cell or progenitor cell populations, which provide an important source of stem cell-based investigation. Nowadays, much work has been done on dermal-derived mesenchymal stem cells (DMSCs) from humans, mice, sheep, and other mammals, but the literature on avian species has been rarely reported. As an animal model, the goose is an endemic species abounding in dermal tissues which is important in the global economy...
May 21, 2018: Poultry Science
https://www.readbyqxmd.com/read/29790687/expression-profiles-of-selected-genes-in-tumors-and-matched-surgical-margins-in-oral-cavity-cancer-do-we-have-to-pay-attention-to-the-molecular-analysis-of-the-surgical-margins
#7
Joanna K Strzelczyk, Łukasz Krakowczyk, Karolina Gołąbek, Aleksander J Owczarek
BACKGROUND: Head and neck squamous cell carcinomas (HNSCCs) are associated with an interplay between genetics and the environment; they account for 3% of all diagnosed malignant tumors in men and 2% of those in women. OBJECTIVES: The aim of the study was to analyze the significance of TIMP3, SFRP1, SFRP2, CDH1, RASSF1, RORA, and DAPK1 gene expression in head and neck squamous cell carcinoma tumors, and in matching surgical margin samples. We also analyzed the association between clinical parameters and the expression of the selected genes...
April 24, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/29790198/the-role-of-lysosomes-and-autophagosomes-in-frontotemporal-lobar-degeneration
#8
Hamish Dc Bain, Yvonne S Davidson, Andrew C Robinson, Sarah Ryan, Sara Rollinson, Anna Richardson, Matthew Jones, Julie S Snowden, Stuart Pickering-Brown, David M A Mann
INTRODUCTION: Cell biological and genetic evidence implicates failures in degrading aggregating proteins, such as tau and TDP-43, through autophagy or lysosomal pathways in the pathogenesis of Frontotemporal Lobar degeneration (FTLD). METHODS: We investigated changes in degradative pathways in 60 patients with different pathological or genetic forms of FTLD employing immunohistochemistry for marker proteins such as Lysosomal Associated Membrane Proteins 1 (LAMP-1) and 2 (LAMP-2), Cathepsin D (CTSD) and microtubule-associated protein 1 light chain 3 alpha (LC3A)...
May 23, 2018: Neuropathology and Applied Neurobiology
https://www.readbyqxmd.com/read/29790107/the-role-of-pharmacogenomics-in-bipolar-disorder-moving-towards-precision-medicine
#9
REVIEW
Claudia Pisanu, Urs Heilbronner, Alessio Squassina
Bipolar disorder (BD) is a common and disabling psychiatric condition with a severe socioeconomic impact. BD is treated with mood stabilizers, among which lithium represents the first-line treatment. Lithium alone or in combination is effective in 60% of chronically treated patients, but response remains heterogenous and a large number of patients require a change in therapy after several weeks or months. Many studies have so far tried to identify molecular and genetic markers that could help us to predict response to mood stabilizers or the risk for adverse drug reactions...
May 22, 2018: Molecular Diagnosis & Therapy
https://www.readbyqxmd.com/read/29790070/genotyping-single-sperm-cells-by-universal-marsala-enables-the-acquisition-of-linkage-information-for-combined-pre-implantation-genetic-diagnosis-and-genome-screening
#10
Haitao Wu, Xiaoting Shen, Lei Huang, Yanhong Zeng, Yumei Gao, Lin Shao, Baomin Lu, Yiping Zhong, Benyu Miao, Yanwen Xu, Yali Wang, Yubin Li, Luoxing Xiong, Sijia Lu, X Sunney Xie, Canquan Zhou
PURPOSE: This paper aims to investigate the feasibility of performing pre-implantation genetic diagnosis (PGD) and pre-implantation genetic screening (PGS) simultaneously by a universal strategy without the requirement of genotyping relevant affected family members or lengthy preliminary work on linkage analysis. METHODS: By utilizing a universal Mutated Allele Revealed by Sequencing with Aneuploidy and Linkage Analyses (MARSALA) strategy based on low depth whole genome sequencing (~3x), not involving specific primers' design nor the enrichment of SNP markers for haplotype construction...
May 22, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29789312/development-of-diagnostic-snp-markers-to-monitor-hybridization-between-sika-deer-cervus-nippon-and-wapiti-cervus-elaphus
#11
Hengxing Ba, Zhipeng Li, Yifeng Yang, Chunyi Li
Sika deer ( Cervus Nippon ) and wapiti ( Cervus elaphus ) are closely related species and their hybridization can result in significant allele-shift of their gene pool. Additive genetic effects and putative heterotic effects of their hybridization on growth performance could confer considerable economic advantage in deer farming. Here, we used double-digest restriction site-associated DNA sequencing technology (ddRAD-seq) and detected ∼320,000 genome-wide SNPs from 30 captive individuals: 7 sika deer, 6 wapiti and 17 F1 hybrids (reciprocal cross)...
May 22, 2018: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29788929/resveratrol-induces-autophagy-dependent-apoptosis-in-hl-60-cells
#12
Yingying Fan, Jen-Fu Chiu, Jing Liu, Yan Deng, Cheng Xu, Jun Zhang, Guanwu Li
BACKGROUND: All known mechanisms of apoptosis induced by resveratrol act through cell cycle arrest and changes in mitochondrial membrane potential. It is currently unknown whether resveratrol-induced apoptosis is associated with other physiological processes, such as autophagy. METHODS: Apoptosis-related markers involved in the intrinsic and extrinsic apoptotic pathways, and autophagic markers were detected by using western blotting and immunofluorescence. Mitochondrial membrane potential was assayed by flow cytometry...
May 22, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29788925/de-novo-transcriptome-assembly-of-the-eight-major-organs-of-sacha-inchi-plukenetia-volubilis-and-the-identification-of-genes-involved-in-%C3%AE-linolenic-acid-metabolism
#13
Xiao-Di Hu, Bang-Zhen Pan, Qiantang Fu, Longjian Niu, Mao-Sheng Chen, Zeng-Fu Xu
BACKGROUND: Sacha Inchi (Plukenetia volubilis L.), which belongs to the Euphorbiaceae, has been considered a new potential oil crop because of its high content of polyunsaturated fatty acids in its seed oil. The seed oil especially contains high amounts of α-linolenic acid (ALA), which is useful for the prevention of various diseases. However, little is known about the genetic information and genome sequence of Sacha Inchi, which has largely hindered functional genomics and molecular breeding studies...
May 22, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29788904/genetic-diversity-of-bola-drb3-in-south-american-zebu-cattle-populations
#14
Shin-Nosuke Takeshima, Claudia Corbi-Botto, Guillermo Giovambattista, Yoko Aida
BACKGROUND: Bovine leukocyte antigens (BoLAs) are used extensively as markers of disease and immunological traits in cattle. However, until now, characterization of BoLA gene polymorphisms in Zebu breeds using high resolution typing methods has been poor. Here, we used a polymerase chain reaction sequence-based typing (PCR-SBT) method to sequence exon 2 of the BoLA class II DRB3 gene from 421 cattle (116 Bolivian Nellore, 110 Bolivian Gir, and 195 Peruvian Nellore-Brahman). Data from 1416 Taurine and Zebu samples were also included in the analysis...
May 22, 2018: BMC Genetics
https://www.readbyqxmd.com/read/29788407/deregulation-of-sgk1-in-ulcerative-colitis-a-paradoxical-relationship-between-immune-cells-and-colonic-epithelial-cells
#15
Rocco Spagnuolo, Vincenzo Dattilo, Lucia D'Antona, Cristina Cosco, Rossana Tallerico, Valeria Ventura, Francesco Conforti, Caterina Camastra, Rosellina M Mancina, Giada Catalogna, Vincenzo Cosco, Rodolfo Iuliano, Ennio Carbone, Nicola Perrotti, Rosario Amato, Patrizia Doldo
Background: Inflammatory bowel disease (IBD) is due to the interaction of genetic and environmental factors that trigger an unbalanced immune response ultimately resulting in the peculiar inflammatory reaction. Experimental models of IBD point to a role of T-cell-derived cytokines (Th17) and to SGK1 as mediator of the Th17 switch. We hypothesize that SGK1, a salt inducible kinase, directs lymphocytic behavior and tissue damage. Methods: Eleven controls and 32 ulcerative colitis (UC) patients were randomized according to endoscopic Mayo score...
May 18, 2018: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/29788324/the-application-of-coi-gene-for-species-identification-of-forensically-important-muscid-flies-diptera-muscidae
#16
Lipin Ren, Wei Chen, Yanjie Shang, Fanming Meng, Lagabaiyila Zha, Yong Wang, Yadong Guo
Muscid Flies (Diptera: Muscidae) are of great forensic importance due to their wide distribution, ubiquitous and synanthropic nature. They are frequently neglected as they tend to arrive at the corpses later than the flesh flies and blow flies. Moreover, the lack of species-level identification also hinders investigation of medicolegal purposes. To overcome the difficulty of morphological identification, molecular method has gained relevance. Cytochrome c oxidase subunit I (COI) gene has been widely utilized...
May 17, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29788122/viewpoint-toward-the-genetic-architecture-of-disease-severity-in-inflammatory-bowel-diseases
#17
Claire Liefferinckx, Denis Franchimont
Inflammatory bowel disease (IBD) is characterized by uneven disease courses with various clinical outcomes. A few prognostic markers of disease severity may help stratify patients and identify those who will benefit the most from early aggressive treatment. The concept of disease severity remains too broad and vague, mainly because the definition must embrace several disease mechanisms, mainly inflammation and fibrosis, with various rates of disease progression. The magnitude of inflammation is an obvious key driver of disease severity in IBD that ultimately influence disease behavior...
May 17, 2018: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/29787827/genetic-variants-of-fatty-acid-elongase-6-in-chinese-holstein-cow
#18
Si Chen, Menglin Chai, Chen Tian, Yuzhuang Li, Tian Deng, Hui Wu, Xiaolin Liu
In the dairy industry, genetic variants have contributed to the improvement of milk production traits. Fatty acid elongase 6 (ELOVL6), which elongates saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs), plays a distinct role in the balance of long-chain fatty acids composition in animals. ELOVL6 catalyzes the elongation of palmitic acids (C16:0) which is the most common saturated fatty acid found in animals and also an essential precursor to synthesize other long-chain fatty acids. However, the genetic variant research of bovine ELOVL6 on milk quality traits is still unknown...
May 19, 2018: Gene
https://www.readbyqxmd.com/read/29787757/dlx2-overexpression-enhanced-accumulation-of-type-ii-collagen-and-aggrecan-by-inhibiting-mmp13-expression-in-mice-chondrocytes
#19
Jianfei Zhang, Wenbin Zhang, Jun Shi, Jiewen Dai, Steve Guofang Shen
Genetic studies revealed a crucial role of Distal-homebox (Dlx) genes in skeletal development, and our previous study demonstrated overexpressing Dlx2 in neural crest cells led to abnormal cartilage structure, including ectopic cartilage in the maxillary region and nasal bone in mice. The aim of this study was to investigate how Dlx2 overexpression affects chondrogenesis in mouse chondroblast cell line TMC23 and the underlying mechanism. We first demonstrated that Dlx2 expression was upregulated during chondrogenesis in TMC23 cells...
May 19, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29787742/hidden-sites-in-the-distribution-of-the-crayfish-plague-pathogen-aphanomyces-astaci-in-eastern-europe-relicts-of-genetic-groups-from-older-outbreaks
#20
Jörn Panteleit, Nina Sophie Keller, Javier Diéguez-Uribeondo, Jenny Makkonen, Laura Martín-Torrijos, Viorica Patrulea, Mălina Pîrvu, Cristina Preda, Anne Schrimpf, Lucian Pârvulescu
The crayfish plague agent Aphanomyces astaci is one of the world's most threatening invasive species. Originally from North America, the pathogen is being imported alongside American crayfish species, which are used for various purposes. In this study, we investigated the marginal, currently known distribution area of the pathogen in Eastern Europe by sampling narrow-clawed crayfish (Astacus leptodactylus) and spiny-cheek crayfish (Orconectes limosus) populations. In addition, using specific real-time PCR, we tested several marine decapod species, which also occur in brackish waters of the Danube at the West coast of the Black Sea and the Dniester River basin...
May 19, 2018: Journal of Invertebrate Pathology
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