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Zemin Li, Xiaolin Wang, Hehai Pan, Hao Yang, Xiang Li, Kuibo Zhang, Hua Wang, Zhaomin Zheng, Hui Liu, Jianru Wang
OBJECTIVE: This study was to investigate whether resistin induces the expression of CCL4 during IVDD and whether Toll-like receptor-4 (TLR-4) and the NF-κB signaling pathway are involved in this process. METHODS: The expression pattern of resistin and CCL4 in different degenerated human NP tissues were measured by qPCR; Effect of resistin on the migration of macrophages was measured by cell migration assay. Resistin-induced CCL4 expression were analyzed by qPCR, ELISA and cell immunofluorescence...
October 10, 2016: Osteoarthritis and Cartilage
Shou-Hua Wang, Fei Ma, Zhao-Hui Tang, Xiao-Cai Wu, Qiang Cai, Ming-Di Zhang, Ming-Zhe Weng, Di Zhou, Jian-Dong Wang, Zhi-Wei Quan
BACKGROUND: Long non-coding RNA (lncRNA) H19 has been reported to involve in many kinds of human cancers and functions as an oncogene. Our previous study found that H19 was over-expressed in gallbladder cancer (GBC) and was shown to promote tumor development in GBC. However, the competing endogenous RNA (ceRNA) regulatory network involving H19 in GBC progression has not been fully elucidated. We aim to detect the role of H19 as a ceRNA in GBC. METHODS AND RESULTS: In this study, the expression of H19 and miR-342-3p were analyzed in 35 GBC tissues and matched normal tissues by using quantitative polymerase chain reaction (qRT-PCR)...
October 3, 2016: Journal of Experimental & Clinical Cancer Research: CR
Hossein Mohammad Rabie, Parviz Malekifar, Mohammad Ali Javadi, Danial Roshandel, Hamed Esfandiari
PURPOSE: To review our experience with crystalline lens extraction and iris claw Artisan IOL implantation in patients with lens subluxation secondary to Marfan syndrome. METHODS: A retrospective analysis of 12 eyes of 9 patients with lens subluxation due to Marfan syndrome who underwent crystalline lens removal and Artisan IOL (Ophtec, Groningen, Netherlands) implantation. A questionnaire of pre- and post-operative data, including demographics, pre- and postoperative comorbidities and complications was completed...
October 5, 2016: International Ophthalmology
Feng Yang, Wenwei Liu, Xiaojuan Yan, Hanyun Zhou, Hongshen Zhang, Jianfei Liu, Ming Yu, Xiaoshan Zhu, Kezhong Ma
BACKGROUND This study investigated how miR-21 expression is reflected in acute myocardial infarction and explored the role of miR-21 and the PTEN/VEGF signaling pathway in cardiac microvascular endothelial cells. MATERIAL AND METHODS We used an in vivo LAD rat model to simulate acute myocardial infarction. MiR-21 mimics and miR-21 inhibitors were injected and transfected into model rats in order to alter miR-21 expression. Cardiac functions were evaluated using echocardiographic measurement, ELISA, and Masson staining...
October 6, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Giuseppe Spinoglio, Alessandra Marano, Paolo Pietro Bianchi, Fabio Priora, Luca Matteo Lenti, Ferruccio Ravazzoni, Giampaolo Formisano
BACKGROUND: A modified complete mesocolic excision (mCME) technique for the treatment of right-sided colon cancer recently was shown by Hohenberger and colleagues to provide impressive long-term oncologic outcomes. This report aims to describe the authors' experience with robotic right colectomy using mCME. The safety, feasibility, and efficacy of this procedure are measured by complications, conversion rates, and 4-year oncologic outcomes. METHODS: A retrospective study analyzed 100 consecutive patients who underwent robotic right colectomy with mCME and intracorporeal anastomosis at the authors' institution between November 2005 and November 2013...
October 3, 2016: Annals of Surgical Oncology
Paula Bompastor-Ramos, João Póvoa, Conceição Lobo, Alejandra E Rodriguez, Jorge L Alió, Liliana Werner, Joaquim N Murta
PURPOSE: To report late postoperative opacification of a model of hydrophilic-hydrophobic acrylic intraocular lens (IOL) as well as the clinical consequences and laboratory characteristics. SETTING: Department of Ophthalmology, Centro Hospitalar e Universitário de Coimbra, Coimbra, Portugal. DESIGN: Retrospective case series. METHODS: Medical records were reviewed of patients with Lentis LS-502-1 IOL opacification reporting visual loss who had IOL explantation between November 2013 and March 2015...
September 2016: Journal of Cataract and Refractive Surgery
Aeri Yoo, Joo Young Kwag, In Seok Song, Myoung Joon Kim, Hyerin Jeong, Jae Yong Kim, Hungwon Tchah
PURPOSE: To compare visual function after implantation of multifocal Lentis comfort LS-313 MF 15 (group 1) or Lentis M plus LS-313 MF 30 (group 2) intraocular lenses (IOLs). METHODS: Patients between 49 and 76 years of age who received monocular cataract surgery with multifocal IOL implantation were analyzed. Patients were evaluated preoperatively and for 5 months postoperatively for distance, intermediate, and near visual acuities, and static photopic and mesopic contrast sensitivity...
September 7, 2016: European Journal of Ophthalmology
Jisoo Park, Quangdon Tran, Kisun Mun, Kouhei Masuda, So Hee Kwon, Seon-Hwan Kim, Dong-Hoon Kim, George Thomas, Jongsun Park
The major biological function of mitochondria is to generate cellular energy through oxidative phosphorylation. Apart from cellular respiration, mitochondria also play a key role in signaling processes, including aging and cancer metabolism. It has been shown that S6K1-knockout mice are resistant to obesity due to enhanced beta-oxidation, with an increased number of large mitochondria. Therefore, in this report, the possible involvement of S6K1 in regulating mitochondria dynamics and function has been investigated in stable lenti-shS6K1-HeLa cells...
December 2016: Cellular Signalling
Rakhi Kusumesh, Anita Ambastha
No abstract text is available yet for this article.
July 2016: Journal of Clinical and Diagnostic Research: JCDR
Maria Elizabeth Cavalheiros Dorval, Elisa Teruya Oshiro, Andreia Fernandes Brilhante, Vânia Lúcia Brandão Nunes, Geucira Cristaldo, Manoel Sebastião Costa Lima Júnior, Eunice Aparecida Bianchi Galati
The phlebotomine fauna of Campo Grande city, capital of Mato Grosso do Sul state in Brazil, an endemic area for visceral leishmaniasis, has been thoroughly investigated, but all the insect collections were undertaken with automatic light traps. The present study sought to investigate the fauna in this city using Shannon and Disney traps, having human beings and hamsters, respectively, as bait. Both types of traps were installed in forest fragment and peridomiciliary areas in the period from 2007 to 2009. The phlebotomine females were analyzed by PCR for Leishmania identification...
2016: Parasite: Journal de la Société Française de Parasitologie
Shaobing Cheng, Xu Jiang, Chaofeng Ding, Chengli Du, Kwabena Gyabaah Owusu-Ansah, Xiaoyu Weng, Wendi Hu, Chuanhui Peng, Zhen Lv, Rongliang Tong, Heng Xiao, Haiyang Xie, Lin Zhou, Jian Wu, Shusen Zheng
Interleukin enhancer binding factor 2 (ILF2), a transcription factor, regulates cell growth by inhibiting the stabilization of mRNA. Currently, its role has gained recognition as a factor in the tumorigenic process. However, until now, little has been known about the detailed role ILF2 plays in hepatocellular carcinoma (HCC). In this study, we investigated the expression levels of ILF2 in HCC tissue with Western blot and immunohistochemical assays. To examine the effect of ILF2 on liver cancer cell growth and apoptosis, small interfering RNAs (siRNAs) targeting ILF2 were recombined to create lentiviral overexpression vectors...
2016: International Journal of Molecular Sciences
Ran Li, Han Wu, Jun Xie, Guannan Li, Rong Gu, Lina Kang, Lian Wang, Biao Xu
Chemotactic migration of endothelial cells (ECs) guided by extracellular attractants is essential for blood vessel formation. Synd4 is a ubiquitous heparin sulfate proteoglycan receptor on the cell surface that has been identified to promote angiogenesis during tissue repair. Here, the role synd4 played in chemotactic migration of ECs was investigated in vitro and in vivo. Human umbilical vein endothelial cells (HUVECs) were transfected with Lenti-synd4-RNAi or Lenti-null. Cell migration was observed in a 2D-chemotaxis slide with a stable gradient of basic fibroblast growth factor (bFGF) for 18 h using time-lapse microscopy...
October 2016: Journal of Molecular Histology
Shu-Biao Ye, Han Zhang, Ting-Ting Cai, Yi-Na Liu, Jian-Jiao Ni, Jia He, Jing-Yun Peng, Qiu-Yan Chen, Hao-Yuan Mo, Jun-Cui, Xiao-Shi Zhang, Yi-Xin Zeng, Jiang Li
Recent studies have shown that extracellular microRNAs are not only potential biomarkers but are also involved in cells' interactions to regulate the intercommunication between cancer cells and their microenvironments in various types of malignancies. In this study, we isolated exosomes from nasopharyngeal carcinoma (NPC) cell lines and patient sera (T-EXO), or control NP69 cells and healthy donor sera (HD-EXO). We found that miR-24-3p was markedly enriched in T-EXO compared with HD-EXO; the serum exosomal miR-24-3p level was correlated with worse disease-free survival of patients (DFS, P < 0...
August 19, 2016: Journal of Pathology
Jaume Català-Mora, Daniel Cuadras, Jesús Díaz-Cascajosa, Marta Castany-Aregall, Joan Prat-Bartomeu, José García-Arumí
PURPOSE: To report the feasibility and long-term safety of lensectomy and iris-claw intraocular lens (IOL) implantation to treat children with severe ectopia lentis in a paediatric tertiary hospital. METHODS: Prospective cohort study of 21 eyes from 12 patients with severe ectopia lentis and visual acuity <20/63. All eyes underwent 23-gauge pars plana vitrectomy, lensectomy, iridectomy and Artisan IOL implantation in the anterior chamber with iris-claw enclavation via pars plana...
August 18, 2016: Acta Ophthalmologica
Richard N McNeely, Eric Pazo, Zack Millar, Olivier Richoz, Andrew Nesbit, Tara C B Moore, Jonathan E Moore
PURPOSE: To determine the degree of tolerance toward different magnitudes of residual refractive astigmatism and corneal astigmatism and the angles of corneal astigmatism after implantation of an asymmetric multifocal intraocular lens (IOL). SETTING: Cathedral Eye Clinic, Belfast, United Kingdom. DESIGN: Retrospective comparative case series. METHODS: The study enrolled patients having refractive lens exchange and implantation of a Lentis Mplus LS-312 MF30 IOL...
August 2016: Journal of Cataract and Refractive Surgery
J H Zhang, X H Kang, P Lu, Z H Miao, G S Sun, X J Cao, F Cao
OBJECTIVE: To investigate the significance of long non-coding RNA MALAT1 expression in osteosarcoma, and the potential mechanism by which MALAT1 promotes tumor metastasis. METHODS: Twenty cases of osteosarcoma in the First Affiliated Hospital of Xinxiang Medical University and Ping Ding Shan First People's Hospital were collected from January 2014 to December 2015. The expression of MALAT1 in osteosarcoma tissue and paired adjacent noncancerous tissue were analyzed by qRT-PCR...
August 8, 2016: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
A Mazza, S Lenti, L Schiavon, E Ramazzina, D Bernardini, E Casiglia
OBJECTIVE: Blood pressure (BP) control is difficult to achieve with current BP-lowering drugs. Therefore, uncontrolled hypertension (HT) despite drug treatment with free recommended combinations remains the main goal in clinical practice. In this study the efficacy of a triple fixed-combination (TFC) of anti-hypertensive drugs in hypertensive subjects with uncontrolled HT was evaluated.(Figure is included in full-text article.) DESIGN AND METHOD: : Forty-one hypertensives (mean age 59...
September 2016: Journal of Hypertension
Cheng-Di Liu, Qin Wang, De-Kang Zong, Shuang-Chao Pei, Yan Yan, Mei-Ling Yan, Lin-Lin Sun, Yang-Yang Hao, Meng Mao, Wen-Jing Xing, Huan Ren, Jing Ai
Reduction of protein phosphatase-2A (PP2A) activity is a common clinical feature of Alzheimer's disease and vascular dementia. In this study, we observed that chronic brain hypoperfusion induced by bilateral common carotid artery occlusion of rats led to PP2A inactivation based on the increase in tyrosine-307 phosphorylation and leucine-309 demethylation of PP2AC and the depression in PP2ABα. Knockdown of miR-195 using overexpression of its antisense molecule oligonucleotide (pre-AMO-miR-195) delivered by a lentivirus (lenti-pre-AMO-miR-195) increased tyrosine-307 phosphorylation and decreased both PP2ABα expression and leucine-309 methylation; these effects were prevented by the overexpression of miR-195 using lenti-pre-miR-195 and controlled by an increase in methylesterase (PME-1) and a decrease in leucine carboxyl methyltransferase-1...
September 2016: Neurobiology of Aging
Zhen-Yu Zhang, Jia-Jun Cai, Jin Hong, Kay Ka-Wai Li, Zhou Ping, Yin Wang, Ho-Keung Ng, Yu Yao, Ying Mao
Studies have showed the involvement of ubiquitin-like with PHD and RING finger domains 1 (UHRF1) in tumorigenesis and progression. This study focused on the relationships between UHRF1 and medulloblastoma (MB). Immunostaining and western blotting demonstrated differential expression of UHRF1 in MB tissues and no UHRF1 expression in normal cerebellum tissues. Univariate survival analysis revealed MB patients with high UHRF1 expression had significantly shorter OS and PFS than patients with low UHRF1 (OS p = 0...
September 2016: Medical Oncology
Lynn Y Sakai, Douglas R Keene, Marjolijn Renard, Julie De Backer
FBN1 encodes the gene for fibrillin-1, a structural macromolecule that polymerizes into microfibrils. Fibrillin microfibrils are morphologically distinctive fibrils, present in all connective tissues and assembled into tissue-specific architectural frameworks. FBN1 is the causative gene for Marfan syndrome, an inherited disorder of connective tissue whose major features include tall stature and arachnodactyly, ectopia lentis, and thoracic aortic aneurysm and dissection. More than one thousand individual mutations in FBN1 are associated with Marfan syndrome, making genotype-phenotype correlations difficult...
October 10, 2016: Gene
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