keyword
MENU ▼
Read by QxMD icon Read
search

Mesenchymal stem cells osteoarthritis

keyword
https://www.readbyqxmd.com/read/29328479/the-human-umbilical-cord-stem-cells-improve-the-viability-of-oa-degenerated-chondrocytes
#1
Hao Wang, Xu Yan, Yuxin Jiang, Zheng Wang, Yufei Li, Qingdong Shao
Osteoarthritis (OA) affects a large number of patients; however, human umbilical cord stem cells exhibit therapeutic potential for treating OA. The aim of the present study was to explore the interaction between human umbilical cord stem cells and degenerated chondrocytes, and the therapeutic potential of human umbilical cord stem cells on degenerated chondrocytes. Human umbilical cord‑derived mesenchymal stem cells (hUC‑MSCs) were harvested from human umbilical cords, and flow cytometry was used to analyze the surface antigen markers, in addition, chondrogenic, osteogenic and adipogenic differentiation on the cells was investigated...
January 9, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29315262/intraarticularly-injected-mesenchymal-stem-cells-stimulate-anti-inflammatory-molecules-and-inhibit-pain-related-protein-and-chondrolytic-enzymes-in-a-monoiodoacetate-induced-rat-arthritis-model
#2
Toru Ichiseki, Miyako Shimazaki, Yoshimichi Ueda, Shusuke Ueda, Masanobu Tsuchiya, Daisuke Souma, Ayumi Kaneuji, Norio Kawahara
Persistent inflammation is well known to promote the progression of arthropathy. mesenchymal stem cells (MSCs) have been shown to possess anti-inflammatory properties and tissue differentiation potency. Although the experience so far with the intraarticular administration of mesenchymal stem cell (MSC) to induce cartilage regeneration has been disappointing, MSC implantation is now being attempted using various surgical techniques. Meanwhile, prevention of osteoarthritis (OA) progression and pain control remain important components of the treatment of early-stage OA...
January 9, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29314205/cdc42-is-essential-for-both-articular-cartilage-degeneration-and-subchondral-bone-deterioration-in-experimental-osteoarthritis
#3
Xinhua Hu, Xing Ji, Mengting Yang, Shihao Fan, Jirong Wang, Meiping Lu, Wei Shi, Liu Mei, Chengyun Xu, Xueying Fan, Musaddique Hussain, Jingyu Du, Junsong Wu, Ximei Wu
Cdc42, a member of Rho family small GTPases, is critical for cartilage development. We investigated the roles of Cdc42 in osteoarthritis and explored the potential mechanism underlying Cdc42-mediated articular cartilage degeneration and subchondral bone deterioration. Cdc42 is highly expressed in both articular cartilage and subchondral bone in a mouse osteoarthritis model with surgical destabilisation of the medial meniscus (DMM) in the knee joints. Specifically, genetic disruption of Cdc42, knockdown of Cdc42 expression, or inhibition of Cdc42 activity robustly attenuates the DMM-induced destruction, hypertrophy, high expression of matrix metallopeptidase-13 and collagen X, and activation of Stat3 in articular cartilages...
January 3, 2018: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/29313037/soluble-collagen-vi-treatment-enhances-mesenchymal-stem-cells-expansion-for-engineering-cartilage
#4
Piera Smeriglio, Jieun Lee, Nidhi Bhutani
Bone Marrow-derived mesenchymal stem cells (BM-MSC) are an attractive source for cell-based therapies in cartilage injury owing to their efficient differentiation into chondrocytes and their immune-suppressive abilities. However, their clinical use is hampered by a scarcity of cells leading to compromised efficacy. While expansion of human MSC ex vivo can potentially overcome the scarcity of cells, current methods lead to a rapid loss of the stem cell properties. In this study, we report soluble Collagen VI (cartilage pericellular matrix component) as a potential biologic that can expand the MSC population while maintaining the stem cell phenotype as confirmed by expression of the stem cell markers CD105 and CD90...
September 2017: Bioengineering & Translational Medicine
https://www.readbyqxmd.com/read/29312843/update-on-mesenchymal-stem-cell-therapies-for-cartilage-disorders
#5
EDITORIAL
Nikolaos K Paschos, Mackenzie L Sennett
Cartilage disorders, including focal cartilage lesions, are among the most common clinical problems in orthopedic practice. Left untreated, large focal lesions may result in progression to osteoarthritis, with tremendous impact on the quality of life of affected individuals. Current management strategies have shown only a modest degree of success, while several upcoming interventions signify better outcomes in the future. Among these, stem cell therapies have been suggested as a promising new era for cartilage disorders...
December 18, 2017: World Journal of Orthopedics
https://www.readbyqxmd.com/read/29301300/the-impact-of-lipid-types-and-liposomal-formulations-on-osteoblast-adiposity-and-mineralization
#6
Shun-Fu Chang, Chih-Chang Yeh, Pin-Jyun Chen, Hsin-I Chang
Recent studies have demonstrated that fat accumulation in bone cells is detrimental to bone mass. Both adipocytes and osteoblasts are derived from common multipotent mesenchymal stem cells (MSCs) and hence the presence of fat may increase adipocyte proliferation, differentiation and fat accumulation while inhibiting osteoblast differentiation and bone formation. Lipids are common constituents in supramolecular vesicles (e.g., micelles or liposomes) that serve as drug delivery systems. Liposomal formulations such as Meriva® were proven to decrease joint pain and improve joint function in osteoarthritis (OA) patients...
January 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29273938/mesenchymal-stem-cells-injections-for-knee-osteoarthritis-a-systematic-overview
#7
REVIEW
Dan Xing, Qi Wang, Ziyi Yang, Yunfei Hou, Wei Zhang, Yaolong Chen, Jianhao Lin
Numerous systematic reviews investigating the effects of mesenchymal stem cells (MSCs) in treating knee osteoarthritis (OA) have been published with controversial conclusion. The purpose of the overview was (1) to perform an overview of systematic reviews investigating MSCs for knee OA and (2) to synthesize evidence qualitatively to assess confidence in the review findings. A systematic search of systematic reviews published through Aug 2017 was conducted using the MEDLINE, EMBASE and Cochrane Library. The methodological quality and risk of bias of included systematic reviews was assessed by AMSTAR instrument and ROBIS tool, respectively...
December 22, 2017: Rheumatology International
https://www.readbyqxmd.com/read/29243283/intra-articular-injection-of-autologous-adipose-derived-mesenchymal-stem-cells-in-treatment-of-knee-osteoarthritis
#8
Duško Spasovski, Vesna Spasovski, Zoran Baščarević, Maja Stojiljković, Miša Vreća, Marina Andjelković, Sonja Pavlović
BACKGROUND: Osteoarthritis (OA) is a chronic degenerative joint disease, considered to be the fourth leading cause of disability and the second cause of inability to work in men. Recently, adipose derived mesenchymal stem cells (AD-MSCs) came in focus of regenerative medicine as promising tool for treatment of OA. It has been shown that administration of stem cells into impaired joints results in pain relief and improves quality of life, accompanied with restoration of hyaline articular cartilage...
December 15, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/29241192/microrna-92a-3p-regulates-aggrecanase-1-and-aggrecanase-2-expression-in-chondrogenesis-and-il-1%C3%AE-induced-catabolism-in-human-articular-chondrocytes
#9
Guping Mao, Peihui Wu, Ziji Zhang, Zhiqi Zhang, Weiming Liao, Yukang Li, Yan Kang
BACKGROUND/AIMS: Aggrecanase-1 (ADAMTS-4) and aggrecanase-2 (ADAMTS-5) are secreted enzymes belonging to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family that play significant roles in the progression of osteoarthritis (OA). Here, we aimed to determine whether the expression of ADAMTS-4/5 in chondrogenesis and inflammation is regulated by microRNA-92a-3p (miR-92a-3p). METHODS: MiR-92a-3p and ADAMTS-4/5 expressions were determined by quantitative polymerase chain reaction (qPCR)...
November 3, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29238343/thrombospondin-1-partly-mediates-the-cartilage-protective-effect-of-adipose-derived-mesenchymal-stem-cells-in-osteoarthritis
#10
Marie Maumus, Cristina Manferdini, Karine Toupet, Paul Chuchana, Louis Casteilla, Mélanie Gachet, Christian Jorgensen, Gina Lisignoli, Danièle Noël
Objective: Assuming that mesenchymal stem cells (MSCs) respond to the osteoarthritic joint environment to exert a chondroprotective effect, we aimed at investigating the molecular response setup by MSCs after priming by osteoarthritic chondrocytes in cocultures. Methods: We used primary human osteoarthritic chondrocytes and adipose stem cells (ASCs) in mono- and cocultures and performed a high-throughput secretome analysis. Among secreted proteins differentially induced in cocultures, we identified thrombospondin-1 (THBS1) as a potential candidate that could be involved in the chondroprotective effect of ASCs...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29237492/pentosan-polysulfate-binds-to-stro-1-mesenchymal-progenitor-cells-is-internalized-and-modifies-gene-expression-a-novel-approach-of-pre-programing-stem-cells-for-therapeutic-application-requiring-their-chondrogenesis
#11
Jiehua Wu, Susan Shimmon, Sharon Paton, Christopher Daly, Tony Goldschlager, Stan Gronthos, Andrew C W Zannettino, Peter Ghosh
BACKGROUND: The pharmaceutical agent pentosan polysulfate (PPS) is known to induce proliferation and chondrogenesis of mesenchymal progenitor cells (MPCs) in vitro and in vivo. However, the mechanism(s) of action of PPS in mediating these effects remains unresolved. In the present report we address this issue by investigating the binding and uptake of PPS by MPCs and monitoring gene expression and proteoglycan biosynthesis before and after the cells had been exposed to limited concentrations of PPS and then re-established in culture in the absence of the drug (MPC priming)...
December 13, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29233163/intra-articular-injection-of-expanded-autologous-bone-marrow-mesenchymal-cells-in-moderate-and-severe-knee-osteoarthritis-is-safe-a-phase-i-ii-study
#12
Mahasen Al-Najar, Hiba Khalil, Jihad Al-Ajlouni, Eman Al-Antary, Mohammad Hamdan, Reem Rahmeh, Dana Alhattab, Osama Samara, Mohamad Yasin, Amenah Al Abdullah, Esraa Al-Jabbari, Dima Hmaid, Hanan Jafar, Abdalla Awidi
BACKGROUND: Knee osteoarthritis (KOA) is a major health problem especially in the aging population. There is a need for safe treatment that restores the cartilage and reduces the symptoms. The use of stem cells is emerging as a possible option for the moderate and severe cases. This study aimed at testing the safety of autologous bone marrow mesenchymal stem cells (BM-MSCs) expanded in vitro when given intra-articularly to patients with stage II and III KOA. As a secondary end point, the study tested the ability of these cells to relieve symptoms and restore the knee cartilage in these patients as judged by normalized knee injury and Osteoarthritis Outcome Score (KOOS) and by magnetic resonance imaging (MRI)...
December 12, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/29230269/extracellular-vesicles-from-adipose-derived-mesenchymal-stem-cells-downregulate-senescence-features-in-osteoarthritic-osteoblasts
#13
Miguel Tofiño-Vian, Maria Isabel Guillén, María Dolores Pérez Del Caz, Miguel Angel Castejón, Maria José Alcaraz
Osteoarthritis (OA) affects all articular tissues leading to pain and disability. The dysregulation of bone metabolism may contribute to the progression of this condition. Adipose-derived mesenchymal stem cells (ASC) are attractive candidates in the search of novel strategies for OA treatment and exert anti-inflammatory and cytoprotective effects on cartilage. Chronic inflammation in OA is a relevant factor in the development of cellular senescence and joint degradation. In this study, we extend our previous observations of ASC paracrine effects to study the influence of conditioned medium and extracellular vesicles from ASC on senescence induced by inflammatory stress in OA osteoblasts...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29199605/modeling-mesenchymal-stem-cells-in-tmj-rheumatoid-arthritis-and-osteoarthritis-therapy
#14
Nedime Serakinci, Gokce Savtekin
Stem cells have self-renewal capacity and an ability to differentiate into particular cell types generating mature cells. Mesenchymal stem cells (MSCs) have a significant role in tissue homeostasis, which leads into tissue regeneration. MSCs are rare pluripotent cells supporting hematopoietic and mesenchymal cell lineages. MSCs are also believed to have therapeutic power over temporomandibular joint (TMJ) disorders (TMDs). The most common type of TMD is articular disc displacement, which induces progressive degenerative changes...
2017: Critical Reviews in Eukaryotic Gene Expression
https://www.readbyqxmd.com/read/29190645/cd105-mesenchymal-stem-cells-migrate-into-osteoarthritis-joint-an-animal-model
#15
Pablo Fernandez-Pernas, Iván Rodríguez-Lesende, Alexandre de la Fuente, Jesús Mateos, Isaac Fuentes, Javier De Toro, Fco J Blanco, M C Arufe
Mesenchymal stem cells are being the focus of connective tissue technology and regenerative medicine, presenting a good choice cell source for improving old and well recognized techniques of cartilage defect repair. For instance, the autologous chondrocyte transplantation using new concepts of regenerative medicine. The present study investigated the risk of xenogenicity of human synovial membrane-derived MSCs, injected into the monkeys using intravenous and intra-articular administration. The animal models used were adult monkeys Rhesus which had been injured into the left knee to create an Osteoarthritis (OA) animal model...
2017: PloS One
https://www.readbyqxmd.com/read/29176667/mesenchymal-stem-cells-derived-exosomes-and-microparticles-protect-cartilage-and-bone-from-degradation-in-osteoarthritis
#16
Stella Cosenza, Maxime Ruiz, Karine Toupet, Christian Jorgensen, Danièle Noël
Mesenchymal stem or stromal cells (MSCs) exert chondroprotective effects in preclinical models of osteoarthritis (OA). Most of their therapeutic effects are mediated via soluble mediators, which can be conveyed within extracellular vesicles (EVs). The objective of the study was to compare the respective role of exosomes (Exos) or microvesicles/microparticles (MPs) in OA. MPs and Exos were isolated from bone marrow murine BM-MSCs through differential centrifugation. Effect of MPs or Exos was evaluated on OA-like murine chondrocytes and chondroprotection was quantified by RT-qPCR...
November 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29171124/kdm6a-promotes-chondrogenic-differentiation-of-periodontal-ligament-stem-cells-by-demethylation-of-sox9
#17
Pingting Wang, Yanjing Li, Tingting Meng, Junjiang Zhang, Yuanyuan Wei, Zhaosong Meng, Yunfeng Lin, Dayong Liu, Lei Sui
OBJECTIVES: KDM6A has been demonstrated critical in the regulation of cell fates. However, whether KDM6A is involved in cartilage formation remains unclear. In this study, we investigated the role of KDM6A in chondrogenic differentiation of PDLSCs, as well as the underlying epigenetic mechanisms. METHODS: KDM6A shRNA was transfected into PDLSCs by lentivirus. The chondrogenic differentiation potential of PDLSCs was assessed by Alcian blue staining. Immunofluorescence was performed to demonstrate H3K27me3 and H3K4me3 levels during chondrogenesis...
November 23, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/29164146/extracorporeal-shock-wave-rebuilt-subchondral-bone-in-vivo-and-activated-wnt5a-ca2-signaling-in-vitro
#18
Lai Yu, Shuitao Liu, Zhe Zhao, Lin Xia, Haochong Zhang, Jing Lou, Jun Yang, Gengmei Xing, Gengyan Xing
Background: This study aimed to identify the optimal extracorporeal shock wave (ESW) intensity and to investigate its effect on subchondral bone rebuilt in vivo and Wnt5a/Ca2+ signaling in vitro using an osteoarthritis (OA) rat model and bone marrow mesenchymal stem cells (BMMSCs), respectively. Methods: OA rats treated with (OA + ESW group) or without (OA group) ESW (n = 12/group) were compared with healthy controls (control group, n = 12). Gait patterns and subchondral trabecular bone changes were measured...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29156945/modulation-of-gene-expression-in-infrapatellar-fat-pad-derived-mesenchymal-stem-cells-in-osteoarthritis
#19
Beatriz Bravo, Jose Manuel Argüello, Arancha R Gortazar, Francisco Forriol, Javier Vaquero
Aim In the osteoarthritis (OA) disease, all structures of the joint are involved. The infrapatellar Hoffa fat pad is rich in macrophages and granulocytes, which also represents a source of adipose mesenchymal progenitor cells (ASC) cells. In our study, we analyze how OA affects the ability of ASC-derived from Hoffa's fat pad to differentiate into chondrocytes. Material and methodology We took knee Hoffa's pad samples and adipose tissue from the proximal thigh from 6 patients diagnosed with severe OA and from another 6 patients with an anterior cruciate ligament (ACL) rupture without OA...
January 2018: Cartilage
https://www.readbyqxmd.com/read/29146943/cryopreservation-of-human-mesenchymal-stem-cells-in-an-allogeneic-bioscaffold-based-on-platelet-rich-plasma-and-synovial-fluid
#20
Haritz Gurruchaga, Laura Saenz Del Burgo, Ane Garate, Diego Delgado, Pello Sanchez, Gorka Orive, Jesús Ciriza, Mikel Sanchez, Jose Luis Pedraz
Transplantation of mesenchymal stem cells (MSCs) has emerged as an alternative strategy to treat knee osteoarthritis. In this context, MSCs derived from synovial fluid could provide higher chondrogenic and cartilage regeneration, presenting synovial fluid as an appropriate MSCs source. An allogeneic and biomimetic bioscaffold composed of Platelet Rich Plasma and synovial fluid that preserve and mimics the natural environment of MSCs isolated from knee has also been developed. We have optimized the cryopreservation of knee-isolated MSCs embedded within the aforementioned biomimetic scaffold, in order to create a reserve of young autologous embedded knee MSCs for future clinical applications...
November 16, 2017: Scientific Reports
keyword
keyword
89204
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"