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https://www.readbyqxmd.com/read/28434178/massilia-chloroacetimidivorans-sp-nov-a-chloroacetamide-herbicide-degrading-bacterium-isolated-from-soil
#1
Hyosun Lee, Dong-Uk Kim, Sooyeon Park, Jung-Hoon Yoon, Jong-Ok Ka
A Gram-stain negative, rod-shaped, aerobic bacterial strain, designated TA-C7e(T), was isolated from an agricultural soil obtained from Taean region, Korea. Phylogenetically, the strain was grouped with the genus Massilia and shared 95.33-97.97% 16S rRNA sequence similarity with the type strains of other species of the genus Massilia. The DNA-DNA hybridisation between TA-C7e(T) and Massilia haematophila KACC 13771(T), Massilia suwonesis KACC 12635(T), and Massilia yuzhufengensis KACC 16569(T) revealed a low level of relatedness (<40%)...
April 22, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28434062/nlophoric-3-6-di-substituted-quinoxalin-carbazoles-synthesis-photophysical-properties-and-dft-studies
#2
Rahul D Telore, Amol G Jadhav, Nagaiyan Sekar
Synthesis of novel 3,6-di(substituted quinoxalin) carbazole fluorophores by the condensation of 1,1'-(9-ethyl-9H-carbazole-3,6-diyl)bis(2-bromoethanone) with methyl, chloro and unsubstituted o-phenylenediamine is presented. Synthesized derivatives are well characterized by (1)H NMR, (13)C NMR, FTIR and Mass spectroscopy. Photophysical studies are carried out using solvents of varying polarities revealed positive solvatochromism and intramolecular charge transfer from carbazole (Donor) to quinoxalin (Acceptor)...
April 22, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28433954/lipidomic-profile-in-three-species-of-dinoflagellates-amphidinium-carterae-cystodinium-sp-and-peridinium-aciculiferum-containing-very-long-chain-polyunsaturated-fatty-acids
#3
Tomáš Řezanka, Jaromír Lukavský, Linda Nedbalová, Karel Sigler
This study describes the identification of very long chain polyunsaturated fatty acids (VLCPUFAs) in three strains of dinoflagellates (Amphidinium carterae, Cystodinium sp., and Peridinium aciculiferum). The strains were cultivated and their lipidomic profiles were obtained by high resolution mass spectrometry with the aid of positive and negative electrospray ionization (ESI) mode by Orbitrap apparatus. Hydrophilic interaction liquid chromatography (HILIC/ESI) was used to separate major lipid classes of the three genera of dinoflagellates by neutral loss scan showing the ion [M + H-28:8](+), where 28:8 was octacosaoctaenoic acid, and by precursor ion scanning of ions at m/z 407, which was an ion corresponding to the structure of acyl of 28:8 acid (C27H39COO(-))...
April 20, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28433885/electric-fields-within-clay-materials-how-to-affect-the-adsorption-of-metal-ions
#4
Xiong Li, Hang Li, Gang Yang
Electric fields exist ubiquitously in chemical and biological systems while how to affect the interfacial adsorption processes remain elusive. Here, molecular dynamics simulations are used to understand at a molecular level the adsorption of metal ions at the interface of aqueous solutions and clay materials that are generally endowed with strong electric fields. In absence of electric fields, even Cs(+), one of the strongest adsorbed metal ions, is facile to detach from solvated clay surfaces. Electric fields are critical to construct stable inner-sphere complexes and enhance pronouncedly the adsorption strengths of both inner- and outer-sphere complexes...
April 13, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28433748/the-repertoire-of-epithelial-morphogenesis-on-display-progressive-elaboration-of-drosophila-egg-structure
#5
REVIEW
Juan Carlos Duhart, Travis T Parsons, Laurel A Raftery
Epithelial structures are foundational for tissue organization in all metazoans. Sheets of epithelial cells form lateral adhesive junctions and acquire apico-basal polarity perpendicular to the surface of the sheet. Genetic analyses in the insect model, Drosophila melanogaster, have greatly advanced our understanding of how epithelial organization is established, and how it is modulated during tissue morphogenesis. Major insights into collective cell migrations have come from analyses of morphogenetic movements within the adult follicular epithelium that cooperates with female germ cells to build a mature egg...
April 19, 2017: Mechanisms of Development
https://www.readbyqxmd.com/read/28433720/establishment-of-a-non-invasive-method-for-stress-evaluation-in-farmed-salmon-based-on-direct-fecal-corticoid-metabolites-measurement
#6
Yanran Cao, Ann-Kristin Tveten, Anne Stene
Fish welfare is an important issue for growth of the aquaculture industry. Stress responses represent animal's natural reactions to challenging conditions and could be used as a welfare indicator. Cortisol level is relevant to fish welfare condition, and is a readily measured component of the primary stress response system. Generally, cortisol is measured by blood sampling. However, fish blood cortisol level could be instantly influenced by handling-stress at sampling. Fecal corticoid metabolites (FCM) are a mixture of several different metabolites with a wide range of polarities...
April 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28433463/actin-regulation-by-tropomodulin-and-tropomyosin-in-neuronal-morphogenesis-and-function
#7
REVIEW
Kevin T Gray, Alla S Kostyukova, Thomas Fath
Actin is a profoundly influential protein; it impacts, among other processes, membrane morphology, cellular motility, and vesicle transport. Actin can polymerize into long filaments that push on membranes and provide support for intracellular transport. Actin filaments have polar ends: the fast-growing (barbed) end and the slow-growing (pointed) end. Depolymerization from the pointed end supplies monomers for further polymerization at the barbed end. Tropomodulins (Tmods) cap pointed ends by binding onto actin and tropomyosins (Tpms)...
April 19, 2017: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/28433435/fabric-phase-sorptive-extraction-as-a-reliable-tool-for-rapid-screening-and-detection-of-freshness-markers-in-oranges
#8
M Aznar, S Úbeda, C Nerin, A Kabir, K G Furton
A simple, fast and sensitive analyte extraction method based on fabric phase sorptive extraction (FPSE) followed by gas chromatography-mass spectrometry (GC-MS) and ultra-performance liquid chromatography-quadrupole time of flight mass spectrometry (UPLC-QTOF-MS) analysis was developed for the analysis of 12 volatile compounds that represent most of the principal chemical families possessing different polarities and volatilities. Five FPSE media coated with different sol-gel sorbent chemistries having different polarities and selectivities were studied: long chain poly(dimethylsiloxane) (PDMS), short chain poly(tetrahydrofuran) (PTHF), Carbowax 20M (CW20M), short chain poly(dimethyl siloxane) (SC PDMS) and polyethylene glycol-polypropylene glycol-polyethylene glycol triblock copolymer (PEG-PPG-PEG)...
April 8, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28433199/glycoprotein-non-metastatic-melanoma-protein-b-gpnmb-is-highly-expressed-in-macrophages-of-acute-injured-kidney-and-promotes-m2-macrophages-polarization
#9
Letian Zhou, Hui Zhuo, Huiyu Ouyang, Yexin Liu, Fang Yuan, Lin Sun, Fuyou Liu, Hong Liu
Acute kidney injury (AKI) is an increasingly common disorder that is strongly linked to short- and long-term morbidity and mortality. During AKI process, macrophages, one of the important immune response cells, can polarize into M1 and M2 subtype from M0 subtype. It is well-known that M1 macrophages play a pro inflammatory role while M2 macrophages play an anti-inflammatory role. Glycoprotein non-metastatic melanoma protein b (Gpnmb) is a glycosylated transmembrane protein highly expressed in numerous cells, including osteoblasts, dendritic cells and macrophages...
March 31, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28433198/tumor-promoting-role-of-anti-tumor-macrophages-in-tumor-microenvironment
#10
REVIEW
Kuntal Kanti Goswami, Tithi Ghosh, Sarbari Ghosh, Madhurima Sarkar, Anamika Bose, Rathindranath Baral
Recent advances in tumor biology demand detailed analysis of the complex interaction of tumor cells with their adjacent microenvironment (tumor stroma) to understand the various mechanisms involved in tumor growth and metastasis. Mononuclear phagocytes or macrophages, a type of innate immune cells, defend the organism against infection and injury. On the otherhand, tumor associated macrophages (TAMs) constitute a significant part of the tumor-infiltrating immune cells, have been linked to the growth, angiogenesis, and metastasis of a variety of cancers, most likely through polarization of TAMs to the M2 (alternative) phenotype...
April 13, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28433193/regulation-of-the-catalytic-behavior-of-pullulanases-chelated-onto-nickel-ii-modified-magnetic-nanoparticles
#11
Jianfeng Wang, Zhongmei Liu, Zhemin Zhou
Chelating of pullulanases onto nickel (II)-modified magnetic nanoparticles results in one-step purification and immobilization of pullulanase, and facilitates the commercial application of pullulanase in industrial scale. To improve the catalytic behavior, especially the operational stability, of the nanocatalyst in consecutive batch reactions, we prepared various iminodiacetic acid-modified magnetic nanoparticles differed in surface polarity and spacer length, on which the His6-tagged pullulanases were chelated via nickel ions, and then studied the correlation between the MNPs surface property and the corresponding catalyst behavior...
June 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28433153/polyethylenimine-kappa-carrageenan-micro-arc-oxidation-coating-for-passivation-of-magnesium-alloy
#12
A Golshirazi, M Kharaziha, M A Golozar
The aim of this study was to combine micro-arc oxidation (MAO) and self-assembly technique to improve corrosion resistivity of AZ91 alloy. While a silicate-fluoride electrolyte was adopted for MAO treatment, polyethylenimine (PEI)/kappa carrageenan (KC) self-assembly coating was applied as the second coating layer. Resulted demonstrated the formation of forsterite-fluoride containing MAO coating on AZ91 alloy depending on the voltage and time of anodizing process. Addition of the second PEI/KC coating layer on MAO treated sample effectively enhanced the adhesive strength of MAO coated sample due to filling the pores with polymers and increase in the mechanical interlocking of coating to the substrate...
July 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28433023/rotational-resonance-for-a-heteronuclear-spin-pair-under-magic-angle-spinning-in-solid-state-nmr
#13
Takayuki Kamihara, K Takegoshi
Rotational resonance (R2) is one of the widely applied techniques in solid-state NMR for recoupling a homonuclear dipolar interaction under magic-angle spinning (MAS). R2 occurs as the result of interference between the difference of the chemical shifts and MAS. In this work, we extend R2 to a heteronuclear dipolar interaction in the interaction frame of RF irradiation. Based on the average Hamiltonian theory, we show that the recoupling of the heteronuclear dipolar (I-S) interaction occurs at the recoupling conditions written as ΩI(')±ΩS(')=kωr (k=0,±1,±2), where ΩX(') is the RF offset for spin-X (X = I or S) scaled by RF irradiation...
April 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28433020/introducing-the-mean-field-approximation-to-cdft-mmpol-method-statistically-converged-equilibrium-and-nonequilibrium-free-energy-calculation-for-electron-transfer-reactions-in-condensed-phases
#14
Hiroshi Nakano, Hirofumi Sato
A new theoretical method to study electron transfer reactions in condensed phases is proposed by introducing the mean-field approximation into the constrained density functional theory/molecular mechanical method with a polarizable force field (CDFT/MMpol). The method enables us to efficiently calculate the statistically converged equilibrium and nonequilibrium free energies for diabatic states in an electron transfer reaction by virtue of the mean field approximation that drastically reduces the number of CDFT calculations...
April 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28433013/shape-resonances-virtual-state-and-ramsauer-townsend-minimum-in-the-low-energy-electron-collisions-with-benzene
#15
Alessandra Souza Barbosa, Márcio H F Bettega
In this work, we revisit the low-energy electron scattering by benzene. We employed the Schwinger multichannel method implemented with pseudopotentials to carry out systematic cross section calculations with different schemes of polarization for the resonant and the totally symmetric irreducible representations within the D2h symmetry group. We present integral and differential cross sections for incident electron energies up to 12 eV and discuss the shape resonances and the presence of a Ramsauer-Townsend minimum and a virtual state in the former...
April 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28433011/ramped-amplitude-novel
#16
T V Can, R T Weber, J J Walish, T M Swager, R G Griffin
We present a pulsed dynamic nuclear polarization (DNP) study using a ramped-amplitude nuclear orientation via electron spin locking (RA-NOVEL) sequence that utilizes a fast arbitrary waveform generator (AWG) to modulate the microwave pulses together with samples doped with narrow-line radicals such as 1,3-bisdiphenylene-2-phenylallyl (BDPA), sulfonated-BDPA (SA-BDPA), and trityl-OX063. Similar to ramped-amplitude cross polarization in solid-state nuclear magnetic resonance, RA-NOVEL improves the DNP efficiency by a factor of up to 1...
April 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28432817/factors-involved-in-early-polarization-of-the-anterior-posterior-axis-in-the-milkweed-bug-oncopeltus-fasciatus
#17
Neta Ginzburg, Mira Cohen, Ariel D Chipman
The axes of insect embryos are defined early in the blastoderm stage. Genes involved in this polarization are well known in Drosophila, but less so in other insects, such as the milkweed bug Oncopeltus fasciatus. Using quantitative PCR, we looked at differential expression of several candidate genes for early anterior-posterior patterning and found that none of them are expressed asymmetrically in the early blastoderm. We then used an RNA-Seq approach to identify novel candidate genes that might be involved in early polarization in Oncopeltus...
April 22, 2017: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/28432324/optimization-of-amino-acid-replacement-costs-by-mutational-pressure-in-bacterial-genomes
#18
Paweł Błażej, Dorota Mackiewicz, Małgorzata Grabińska, Małgorzata Wnętrzak, Paweł Mackiewicz
Mutations are considered a spontaneous and random process, which is important component of evolution because it generates genetic variation. On the other hand, mutations are deleterious leading to non-functional genes and energetically costly repairs. Therefore, one can expect that the mutational pressure is optimized to simultaneously generate genetic diversity and preserve genetic information. To check if empirical mutational pressures are optimized in these ways, we compared matrices of nucleotide mutation rates derived from bacterial genomes with their best possible alternatives that minimized or maximized costs of amino acid replacements associated with differences in their physicochemical properties (e...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28432309/fano-resonance-with-high-local-field-enhancement-under-azimuthally-polarized-excitation
#19
Wuyun Shang, Fajun Xiao, Weiren Zhu, Hongsen He, Malin Premaratne, Ting Mei, Jianlin Zhao
Being an enabling technology for applications such as ultrasensitive biosensing and surface enhanced spectroscopy, enormous research interests have been focused on further boosting the local field enhancement at Fano resonance. Here, we demonstrate a plasmonic Fano resonance resulting from the interference between a narrow magnetic dipole mode and a broad electric dipole mode in a split-ring resonator (SRR) coupled to a nanoarc structure. Strikingly, when subjected to an azimuthally polarized beam (APB) excitation, the intensity enhancement becomes more than 60 times larger than that for a linearly polarized beam (LPB)...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28432279/il-35-induces-n2-phenotype-of-neutrophils-to-promote-tumor-growth
#20
Jiu-Ming Zou, Jian Qin, Yong-Chao Li, Yu Wang, Dong Li, Yu Shu, Chao Luo, Shan-Shan Wang, Gang Chi, Fang Guo, Gui-Mei Zhang, Zuo-Hua Feng
IL-35 is an immunosuppressive cytokine and exerts regulatory effects on T cells, B cells, macrophages and dendritic cells. Neutrophils are important innate immune cells that play key roles in tumor development. The effect of IL-35 on neutrophils remains unknown. Here, we report that IL-35 can induce N2 neutrophil polarization (protumor phenotype) by increasing G-CSF and IL-6 production, and promote neutrophil infiltration into tumor microenvironment. The sustained expression of IL-35 could promote chronic inflammation to augment the proangiogenic and immunosuppressive function of neutrophils...
April 4, 2017: Oncotarget
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