keyword
https://read.qxmd.com/read/37706267/analysis-of-the-inhibitory-effect-of-hsa-mir-145-5p-and-hsa-mir-203a-5p-on-imatinib-resistant-k562-cells-by-gc-ms-metabolomics-method
#1
JOURNAL ARTICLE
Priyanka Singh, Radheshyam Yadav, Malkhey Verma, Ravindresh Chhabra
Imatinib (IM) resistance is considered to be a significant challenge in the management of chronic myeloid leukemia (CML). Previous studies have reported that hsa-miR-145-5p and hsa-miR-203a-5p can overcome IM resistance and hsa-miR-203a-5p can alter glutathione metabolism in IM-resistant cells. The purpose of this study was to examine whether hsa-miR-145-5p or hsa-miR-203a-5p counters IM resistance by targeting the overall metabolic profile of IM-resistant K562 cells. The metablic profiling of cell lysates obtained from IM-sensitive, IM-resistant, and miR-transfected IM-resistant K562 cells was carried out using the GC-MS technique...
September 14, 2023: Journal of the American Society for Mass Spectrometry
https://read.qxmd.com/read/37683750/crispr-cas9-mutated-p-coumaroyl-shikimate-3-hydroxylase-3-gene-in-populus-tomentosa-reveals-lignin-functioning-on-supporting-tree-upright
#2
JOURNAL ARTICLE
Sufang Zhang, Bo Wang, Qian Li, Wenkai Hui, Linjie Yang, Zhihua Wang, Wenjuan Zhang, Fengxia Yue, Nian Liu, Huiling Li, Fachuang Lu, Kewei Zhang, Qingyin Zeng, Ai-Min Wu
The lignin plays one of the most important roles in plant secondary metabolism. However, it is still unclear how lignin can contribute to the impressive height of wood growth. In this study, C3'H, a rate-limiting enzyme of the lignin pathway, was used as the target gene. C3'H3 was knocked out by CRISPR/Cas9 in Populus tomentosa. Compared with wild-type popular trees, c3'h3 mutants exhibited dwarf phenotypes, collapsed xylem vessels, weakened phloem thickening, decreased hydraulic conductivity and photosynthetic efficiency, and reduced auxin content, except for reduced total lignin content and significantly increased H-subunit lignin...
September 6, 2023: International Journal of Biological Macromolecules
https://read.qxmd.com/read/37425089/heat-stroke-alters-hippocampal-and-cerebellar-transmitter-metabonomics
#3
JOURNAL ARTICLE
Guoxin He, Ancong Xu, Xichong Yu, Fan Huang, Lei Su
BACKGROUND: The mechanisms underlying heat stroke (HS)-induced hippocampal injury remain unclear. This study aimed to evaluate the HS-induced metabonomics of hippocampal and cerebellar transmitters. METHODS: The HS model was established with male Sprague-Dawley rats subjected to heat exposure of up to 42 °C at a humidity of (55.0±5.0)%. The hippocampal and cerebellar transmitters and metabolites of rats were tested via ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS)...
2023: World Journal of Emergency Medicine
https://read.qxmd.com/read/36929451/integration-of-clinical-and-spatial-data-to-explore-lipid-metabolism-related-genes-for-predicting-prognosis-and-immune-microenvironment-in-gliomas
#4
JOURNAL ARTICLE
Zhangyi Yu, Yuneng Zhou, Yongxue Li, Zhiqiang Dong
Lipid metabolism is crucial to tumor growth and immune microenvironment as well as drug sensitivity in glioma. Identifying prognostic indicators of glioma and elucidating the mechanisms of glioma progression are critical for improving the prognosis of glioma patients. In this study, we investigated the role and prognostic value of metabolism-related genes in glioma by integrative analysis of datasets from GEO, CGGA, and TCGA. Based on clinical data and transcriptome data, we found that the expression pattern of three major pathways related to lipid metabolism is fatty acidhigh -phospholipidhigh -triglyceridelow , which is associated with better prognosis and immune infiltration...
March 16, 2023: Functional & Integrative Genomics
https://read.qxmd.com/read/34403192/plant-metabolic-network-15-a-resource-of-genome-wide-metabolism-databases-for-126-plants-and-algae
#5
JOURNAL ARTICLE
Charles Hawkins, Daniel Ginzburg, Kangmei Zhao, William Dwyer, Bo Xue, Angela Xu, Selena Rice, Benjamin Cole, Suzanne Paley, Peter Karp, Seung Yon Rhee
To understand and engineer plant metabolism, we need a comprehensive and accurate annotation of all metabolic information across plant species. As a step towards this goal, we generated genome-scale metabolic pathway databases of 126 algal and plant genomes, ranging from model organisms to crops to medicinal plants (https://plantcyc.org). Of these, 104 have not been reported before. We systematically evaluated the quality of the databases, which revealed that our semi-automated validation pipeline dramatically improves the quality...
August 17, 2021: Journal of Integrative Plant Biology
https://read.qxmd.com/read/33115253/roles-of-ion-fluxes-metabolism-and-redox-balance-in-cancer-therapy
#6
JOURNAL ARTICLE
Huanling Lai, Xingxing Fan, Runze Li, Yuwei Wang, Junmin Zhang, Liang Liu, Erwin Neher, Xiao-Jun Yao, Elaine Lai-Han Leung
Recent Advances: The 2019 Nobel Prize awarded to the mechanisms for oxygen sensing and adaptation according to oxygen availability, highlighting the fundamental importance of gaseous molecules. Gaseous molecules including reactive oxygen species (ROS) can interact with different cations generated during metabolic and redox dysregulation in cancer cells. Crosstalk between calcium signaling and metabolic/redox pathways leads to network-based dyregulation in cancer. SIGNIFICANCE: Recent discovery on using small molecules targeting the ion channels, redox signaling, and protein modification on metabolic enzymes can effectively inhibit cancer growth...
October 29, 2020: Antioxidants & Redox Signaling
https://read.qxmd.com/read/32656897/development-of-a-method-to-screen-and-isolate-lipoxidase-inhibitors-from-radix-saposhnikoviae-via-ultrafiltration-liquid-chromatography-combined-with-metablism-in-vitro
#7
JOURNAL ARTICLE
Yu Huang, Min Yu, Tong Wu, Wanchao Hou, Chunming Liu, Sainan Li
INTRODUCTION: Radix Saposhnikoviae is an excellent source of chromones, the consumption of which has been shown to positively affect human health, such as anti-inflammatory and antioxidant activities in vivo, and it also has been used to treat headache, inflammation, and cancer as a herbal medicine or functional soup. OBJECTIVE: Isolation and screening of lipoxidase (LOX) inhibitors from Radix Saposhnikoviae. As a result, the metabolism of the active ingredients in cytochrome P450 (CYP450) in vitro was identified...
July 12, 2020: Phytochemical Analysis: PCA
https://read.qxmd.com/read/31115975/mir-145-inhibits-glutamine-metabolism-through-c-myc-gls1-pathways-in-ovarian-cancer-cells
#8
JOURNAL ARTICLE
Jie Li, Xu Li, Lei Wu, Meili Pei, Huijin Li, Yu Jiang
miR-145 has been found to be a participant in cancer metastasis and glucose metabolism in ovarian cancer. However, the role of glutamine metabolism in ovarian cancer remains unclear. In this study, we aim to elucidate the molecular mechanism underlying the regulation of glutamine metabolism by miR-145 in ovarian cancer cells. The messenger RNA (mRNA) levels of miR-145 and glutaminase 1 (GLS1) were examined by quantitative real-time polymerase chain reaction (qRT-PCR). The protein levels of c-myc and GLS1 were detected by western blot analysis...
August 2019: Cell Biology International
https://read.qxmd.com/read/30446179/salts-and-energy-balance-a-special-role-for-dietary-salts-in-metabolic-syndrome
#9
JOURNAL ARTICLE
Christopher W Brey, Safoura Akbari-Alavijeh, Jun Ling, Jordan Sheagley, Bilal Shaikh, Futwan Al-Mohanna, Yi Wang, Randy Gaugler, Sarwar Hashmi
BACKGROUND: Dietary salts sodium (Na+ ), potassium (K+ ), magnesium (Mg2+ ), and calcium (Ca2+ ) are important in metabolic diseases. Yet, we do not have sufficient understanding on the salts global molecular network in these diseases. In this systematic review we have pooled information to identify the general effect of salts on obesity, insulin resistance and hypertension. AIMS: To assess the roles of salts in metabolic disorders by focusing on their individual effect and the network effect among these salts...
October 2019: Clinical Nutrition
https://read.qxmd.com/read/30181762/ppar%C3%AE-pgc-1%C3%AE-fndc5-pathway-up-regulation-in-gastrocnemius-and-heart-muscle-of-exercised-branched-chain-amino-acid-diet-fed-mice
#10
JOURNAL ARTICLE
Navid Abedpoor, Farzaneh Taghian, Kamran Ghaedi, Iman Niktab, Zahra Safaeinejad, Farzaneh Rabiee, Sommayeh Tanhaei, Mohammad Hossein Nasr-Esfahani
Background: Previous studies have revealed the inductive effect of branched-chain amino acids (BCAAs) catabolism on fatty acid oxidation and metabolism, especially in muscle cells. In the present investigation, we have attempted to address whether a combination of BCAAs supplement consumption with aerobic exercise could elaborate the expression of PPAR γ, Pgc-1α and Fndc5 genes in gastrocnemius muscle and heart tissue of male C57BL/6 mice. Methods: Thirty-six young male mice with an average weight of 18 ± 2 g were selected...
2018: Nutrition & Metabolism
https://read.qxmd.com/read/28821013/rictor-positively-regulates-b-cell-receptor-signaling-by-modulating-actin-reorganization-via-ezrin
#11
JOURNAL ARTICLE
Lu Huang, Yongjie Zhang, Chenguang Xu, Xiaomei Gu, Linlin Niu, Jinzhi Wang, Xiaoyu Sun, Xiaoming Bai, Xingtian Xuan, Qubei Li, Chunwei Shi, Bing Yu, Heather Miller, Gangyi Yang, Lisa S Westerberg, Wanli Liu, Wenxia Song, Xiaodong Zhao, Chaohong Liu
As the central hub of the metabolism machinery, the mammalian target of rapamycin complex 2 (mTORC2) has been well studied in lymphocytes. As an obligatory component of mTORC2, the role of Rictor in T cells is well established. However, the role of Rictor in B cells still remains elusive. Rictor is involved in B cell development, especially the peripheral development. However, the role of Rictor on B cell receptor (BCR) signaling as well as the underlying cellular and molecular mechanism is still unknown. This study used B cell-specfic Rictor knockout (KO) mice to investigate how Rictor regulates BCR signaling...
August 2017: PLoS Biology
https://read.qxmd.com/read/28539709/multipathway-integrated-adjustment-mechanism-of-glycyrrhiza-triterpenes-curing-gastric-ulcer-in-rats
#12
JOURNAL ARTICLE
Ying Wang, Shuai Wang, Yongrui Bao, Tianjiao Li, Xin Chang, Guanlin Yang, Xiansheng Meng
BACKGROUND: Gastric ulcer is a common chronic disease in human digestive system, which is difficult to cure, easy to relapse, and endangers human health seriously. Compared with western medicine, traditional Chinese medicine has a unique advantage in improving the general situation, stablizing medical condition, and with little side effects. Glycyrrhiza known as "king of all the medicine", has a range of pharmacological activities and is commonly used in a variety of proprietary Chinese medicines and formulations...
April 2017: Pharmacognosy Magazine
https://read.qxmd.com/read/28432361/disease-biomarker-identification-from-gene-network-modules-for-metastasized-breast-cancer
#13
JOURNAL ARTICLE
Pooja Sharma, Dhruba K Bhattacharyya, Jugal Kalita
Advancement in science has tended to improve treatment of fatal diseases such as cancer. A major concern in the area is the spread of cancerous cells, technically refered to as metastasis into other organs beyond the primary organ. Treatment in such a stage of cancer is extremely difficult and usually palliative only. In this study, we focus on finding gene-gene network modules which are functionally similar in nature in the case of breast cancer. These modules extracted during the disease progression stages are analyzed using p-value and their associated pathways...
April 21, 2017: Scientific Reports
https://read.qxmd.com/read/27715462/capsaicin-reactivates-hmof-in-gastric-cancer-cells-and-induces-cell-growth-inhibition
#14
JOURNAL ARTICLE
Fei Wang, Jiayao Zhao, Da Liu, Tong Zhao, Zeming Lu, Lin Zhu, Lingling Cao, Jiaxing Yang, Jingji Jin, Yong Cai
Capsaicin (CAP) is the major pungent component of chili pepper and is being evaluated for use against numerous types of tumors. Although CAP is indicated to target multiple signaling pathways, exact mechanisms of how it disturb cancer cell metablism remain obscure. Recent studies revealed Sirtuin 1 (SIRT1) serves as a potential target of CAP in cancer cells, indicating a direct regulation of cancer cell histone acetylation by capsaicin. The present study evaluated the effect of CAP on gastric cancer (GC) cell lines to understand the mechanism of cell growth inhibition...
November 2016: Cancer Biology & Therapy
https://read.qxmd.com/read/23671993/-aging-and-aging-intervention-by-coloric-restriction-in-nonhuman-primate-rhesus-monkeys
#15
REVIEW
Qing-Wei Ruan, Zhuo-Wei Yu, Yong-Xing Ma
Nonhuman primate rhesus monkey is a ideal model for human aging and aging intervention. Same with human aging, rehesus monkey demonstrates age-related aging and diseases in endocrine, neural, immune and cardiovascular system. Coloric restriction can slow down the primary and secondry aging efficently. The mechanism may be to suppress the oxidative stress-inflammaging-DNA damage by mediating the nutrient sensitive metablic signal pathways, and activate damaged DNA repair. However, the differeces of study design, husbandry and diet composition may differently affect CR against the the primary and secondry effects in a long-lived nonhuman primate...
February 2013: Sheng Li Ke Xue Jin Zhan [Progress in Physiology]
https://read.qxmd.com/read/16613267/-dynamic-change-of-metabolism-related-protein-in-liver-tissue-of-rats-model-of-hepatic-fibrosis-and-regulatory-effect-of-fuzheng-huayu-decoction-on-it
#16
JOURNAL ARTICLE
Ying Liu, Ping Liu, Yi-Yang Hu, Lieming Xu, Lei Wang, Yongping Mu, Guangli Du
OBJECTIVE: To investigate the effect of Fuzheng Huayu decoction (FHD) intervention on hepatic fibrosis. METHODS: Wistar rats were randomly divided into 3 groups: rats in the normal group only treated with subcutaneous injection of saline, rats in the model group and the FHD group were made into hepatic fibrosis by subcutaneous injection of 40% carbon tetrachloride (CCl4)-olive solution and then those in the FHD group were treated with FHD by gastric perfusion after modeling...
March 2006: Chinese Journal of Integrated Traditional and Western Medicine
https://read.qxmd.com/read/12552812/-studies-on-characteristics-of-kinetics-and-metabolic-shift-of-genetically-engineered-yeast-pichia-pastoris-in-high-density-chemostat-cultivation
#17
JOURNAL ARTICLE
M Guo, K Wu, Y Zhuang, J Chu, S Zhang
Kinetics and metabolic shift of DNA recombinant yeast Pichia pastoris was studied in high-density chemostat cultivation in which the glycerol was used as a limited substrate. The experimental results showed that (1) cell optical density (OD600) of Pichia pastoris was linear with its dry cell weight (DCW) and wet cell weight (WCW), and reached 100 OD600 = 128.3 g WCW/L or 100 OD600 = 22.9 g DCW/L; (2) The relationship of specific growth rate (mu) and residual concentration of glycerol is accorded with Monod Equation, to obtain mu = mumax S/(Ks + S), where mumax = 0...
October 2001: Wei Sheng Wu Xue Bao, Acta Microbiologica Sinica
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