keyword
MENU ▼
Read by QxMD icon Read
search

gene delivery

keyword
https://www.readbyqxmd.com/read/29342168/reduction-in-musca-domestica-fecundity-by-dsrna-mediated-gene-knockdown
#1
Neil D Sanscrainte, Hanayo Arimoto, Christy M Waits, Lucy Y Li, Dana Johnson, Chris Geden, James J Becnel, Alden S Estep
House flies (Musca domestica) are worldwide agricultural pests with estimated control costs at $375 million annually in the U.S. Non-target effects and widespread resistance challenge the efficacy of traditional chemical control. Double stranded RNA (dsRNA) has been suggested as a biopesticide for M. domestica but a phenotypic response due to the induction of the RNAi pathway has not been demonstrated in adults. In this study female house flies were injected with dsRNA targeting actin-5C or ribosomal protein (RP) transcripts RPL26 and RPS6...
2018: PloS One
https://www.readbyqxmd.com/read/29341934/advances-in-the-delivery-of-antisense-oligonucleotides-for-combating-bacterial-infectious-diseases
#2
REVIEW
Xiao-Yan Xue, Xing-Gang Mao, Ying Zhou, Zhou Chen, Yue Hu, Zheng Hou, Ming-Kai Li, Jing-Ru Meng, Xiao-Xing Luo
Discovery and development of new antibacterial drugs against multidrug resistant bacterial strains have become more and more urgent. Antisense oligonucleotides (ASOs) show immense potential to control the spread of resistant microbes due to its high specificity of action, little risk to human gene expression, and easy design and synthesis to target any possible gene. However, efficient delivery of ASOs to their action sites with enough concentration remains a major obstacle, which greatly hampers their clinical application...
January 13, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29341933/the-effects-of-platelet-lysate-patches-on-the-activity-of-tendon-derived-cells
#3
Raquel Costa-Almeida, Albina R Franco, Tamagno Pesqueira, Mariana B Oliveira, Pedro S Babo, Isabel B Leonor, João F Mano, Rui L Reis, Manuela E Gomes
Platelet-derived biomaterials are widely explored as cost-effective sources of therapeutic factors, holding a strong potential for endogenous regenerative medicine. Particularly for tendon repair, treatment approaches that shift the injury environment are explored to accelerate tendon regeneration. Herein, genipin-crosslinked platelet lysate (PL) patches are proposed for the delivery of human-derived therapeutic factors in patch augmentation strategies aiming at tendon repair. Developed PL patches exhibited a controlled release profile of PL proteins, including bFGF and PDGF-BB...
January 13, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29341911/an-efficient-pegylated-gene-delivery-system-with-improved-targeting-synergism-between-octaarginine-and-a-fusogenic-peptide
#4
Ikramy A Khalil, Hideyoshi Harashima
Because of their ability to translocate different cargos into cells, arginine-rich cell penetrating peptides (CPPs) are promising vehicles for drug and gene delivery. The use of CPP-based carriers, however, is hampered by the lack of specificity and by interactions with negative serum components. Polyethylene glycol (PEG) is used to decrease such non-specific interactions, albeit its use is associated with reduced transfection efficiency. In this study, we describe the development of PEGylated CPP-based gene carrier with an improved targeting and a high transfection activity...
January 13, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29341305/plasmid-templated-control-of-dna-cyclodextrin-nanoparticle-morphology-through-molecular-vector-design-for-effective-gene-delivery
#5
Laura Gallego-Yerga, Juan M Benito, Laura Blanco-Fernández, María Martínez-Negro, Itziar Vélaz, Emilio Aicart, Elena Junquera, Carmen Ortiz Mellet, Conchita Tros de Ilarduya, Jose M Garcia Fernandez
Engineering self-assembled superstructures through complexation of plasmid DNA (pDNA) and single-isomer nanometric size macromolecules (molecular nanoparticles) is a promising strategy for gene delivery. Notably, the functionality and overall architecture of the vector can be precisely moulded at the atomic level by chemical tailoring, enabling unprecedented opportunities for structure/self-assembling/pDNA delivery relationship studies. Beyond this notion, by judiciously preorganizing the functional elements in cyclodextrin (CD)-based molecular nanoparticles through covalent dimerization, here we demonstrate that the morphology of the resulting nanocomplexes (CDplexes) can be tuned, from spherical to ellipsoidal, rod-type or worm-like nanoparticles, which makes it possible to gain understanding of their shape-dependent transfection properties...
January 17, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29340644/proteasome-inhibition-increases-the-efficiency-of-lentiviral-vector-mediated-transduction-of-trabecular-meshwork
#6
Zeynep Aktas, Hongyu Rao, Sarah R Slauson, B'Ann T Gabelt, Inna V Larsen, Rachael T C Sheridan, Leonie Herrnberger, Ernst R Tamm, Paul L Kaufman, Curtis R Brandt
Purpose: To determine if proteasome inhibition using MG132 increased the efficiency of FIV vector-mediated transduction in human trabecular meshwork (TM)-1 cells and monkey organ-cultured anterior segments (MOCAS). Methods: TM-1 cells were pretreated for 1 hour with 0.5% dimethyl sulfoxide (DMSO; vehicle control) or 5 to 50 μM MG132 and transduced with FIV.GFP (green fluorescent protein)- or FIV.mCherry-expressing vector at a multiplicity of transduction (MOT) of 20...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29340361/a-curcumin-loaded-polymeric-micelle-as-a-carrier-of-a-microrna-21-antisense-oligonucleotide-for-enhanced-anti-tumor-effects-in-a-glioblastoma-animal-model
#7
Xiaonan Tan, Gyeungyun Kim, Dahee Lee, Jungju Oh, Minkyung Kim, Chunxian Piao, Jaewon Lee, Min Sang Lee, Ji Hoon Jeong, Minhyung Lee
A glioblastoma is a common primary brain tumor that expresses microRNA-21 (miR-21), which inhibits the expression of pro-apoptotic genes such as phosphatase and tensin homologue (PTEN) and programmed cell death 4 (PDCD4). Therefore, an antisense-oligonucleotide against miR-21 (miR21ASO) could have therapeutic effects for glioblastomas. In this study, curcumin was loaded into deoxycholic acid-conjugated polyethylenimine (DP) micelles. The curcumin-loaded DP micelle (DP-Cur) was evaluated as a carrier for the combined delivery of curcumin and miR21ASO...
January 17, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29339969/a-new-toolset-for-protein-expression-and-subcellular-localization-studies-in-citrus-and-its-application-to-citrus-tristeza-virus-proteins
#8
Amit Levy, Choaa El-Mochtar, Chunxia Wang, Michael Goodin, Vladimir Orbovic
Background: Transient gene expression is a powerful tool to study gene function in plants. In citrus, Agrobacterium transformation is the method of choice for transient expression studies, but this method does not work efficiently with many gene constructs, and there is a need for a more robust transient expression system in citrus leaves. Biolistic particle delivery is an alternative to Agrobacterium transformation, and in some plants, such as Arabidopsis, gives higher transformation rates in leaf tissues than Agrobacterium...
2018: Plant Methods
https://www.readbyqxmd.com/read/29339452/effects-of-colostrum-feeding-method-and-oral-igf1-on-porcine-uterine-development
#9
Ashley F George, Kathleen M Rahman, Dori J Miller, Anne A Wiley, Meredith E Camp, Frank Bartol, Carol A Bagnell
Nursing ensures lactocrine delivery of maternally-derived, milk-borne bioactive factors to offspring, which affects postnatal development of female reproductive tract tissues. Disruption of lactocrine communication for two days from birth (postnatal day = PND 0) by feeding milk replacer in lieu of nursing or consumption of colostrum alters porcine uterine gene expression globally by PND 2 and inhibits uterine gland genesis by PND 14. Here, objectives were to determine effects of: 1) nursing or milk replacer feeding from birth; 2) a single dose of colostrum or milk replacer and method of feeding; and 3) a single feeding of colostrum or milk replacer, with or without oral supplementation of IGF1, administered at birth on aspects of porcine uterine development at 12 h postnatally...
January 16, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29339286/chitosan-an-undisputed-bio-fabrication-material-for-tissue-engineering-and-bio-sensing-applications
#10
REVIEW
Anupriya Baranwal, Ashutosh Kumar, A Priyadharshini, Gopi Suresh Oggu, Ira Bhatnagar, Ananya Srivastava, Pranjal Chandra
Biopolymers have been serving the mankind in various ways since long. Over the last few years, these polymers have found great demand in various domains which includes biomedicine, tissue engineering, biosensor fabrications etc. because of their excellent biocompatibility. In this context, chitosan has found global attention due to its environmentally benign nature, biocompatibility, biodegradability, and ease of availability. In last one decade or so, extensive research in active biomaterials, like chitosan has led to the development of novel delivery systems for drugs, genes, and biomolecules; and regenerative medicine...
January 12, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29338433/in-vivo-ovarian-cancer-gene-therapy-using-crispr-cas9-system
#11
Zhi-Yao He, Ya-Guang Zhang, Yu-Han Yang, Cui-Cui Ma, Ping Wang, Wei Du, Ling Li, Rong Xiang, Xiang-Rong Song, Xia Zhao, Shaohua Yao, Yu-Quan Wei
CRISPR-Cas9 genome editing technology holds great promise for the field of human gene therapy. However, deficiency of safe and effective delivery systems restricts the biomedical application of CRISPR-Cas9 technique. Here, we use a folate receptor-targeted liposome (F-LP) to deliver a CRISPR plasmid DNA co-expressing Cas9 and single guide RNA targeting the DNA methyltransferase 1 (DNMT1) gene of ovarian cancer. F-LP binds CRISPR plasmid (gDNMT1) efficiently and the formed lipoplex (F-LP/gDNMT1) is stable and safe for injection...
January 16, 2018: Human Gene Therapy
https://www.readbyqxmd.com/read/29338191/conjugate-polyplexes-with-anti-invasive-properties-and-improved-sirna-delivery-in-vivo
#12
Yi Chen, Jing Li, David Oupický
This study reports on a simple method to prepare siRNA-polycation conjugate polyplexes by in situ thiol-disulfide exchange reaction. The conjugate polyplexes are prepared using thiol-terminated siRNA and a bioreducible branched polycationic inhibitor of the CXCR4 chemokine receptor (rPAMD). The rPAMD-SS-siRNA conjugate polyplexes exhibit improved colloidal stability and resistance against disassembly with heparin, serum, and physiological salt concentrations when compared with control conventional rPAMD/siRNA polyplexes...
January 17, 2018: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29337531/a-branched-glycerol-based-copolymer-with-ultrahigh-p65-sirna-delivery-efficiency-for-enhanced-cancer-therapy
#13
Li Zhou, Xiaoyan Qu, Yi Guo, Min Wang, Bo Lei, Peter X Ma
The small interfering RNA (siRNA) is emerging as a potential therapeutic for the treatment of various diseases, because of the targeted gene silencing capability. The suppression of p65 expression has shown great potential in various cancer treatments. However, various substantial obstacles limit their clinical applications, including low cellular uptake, instability and cytotoxicity of delivery vehicles. We show a highly branched and biocompatible glycerol-based copolymer (HBGCs) to effectively deliver siRNA for excellent p65 gene silencing and safe lung cancer treatment in vitro and in vivo...
January 16, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29337376/crispr-cas9-mediated-genome-editing-in-epstein-barr-virus-transformed-lymphoblastoid-b-cell-lines
#14
Sizun Jiang, Liang Wei Wang, Michael J Walsh, Stephen J Trudeau, Catherine Gerdt, Bo Zhao, Benjamin E Gewurz
Epstein-Barr virus (EBV) efficiently transforms primary human B cells into immortalized lymphoblastoid cell lines (LCLs), which are extensively used in human genetic, immunological and virological studies. LCLs provide unlimited sources of DNA for genetic investigation, but can be difficult to manipulate, for instance because low retroviral or lentiviral transduction frequencies hinder experiments that require co-expression of multiple components. This unit details Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 engineering for robust LCL genome editing...
January 16, 2018: Current Protocols in Molecular Biology
https://www.readbyqxmd.com/read/29337374/pooled-lentiviral-delivery-genetic-screens
#15
Federica Piccioni, Scott T Younger, David E Root
Pooled cell-based screens of mammalian genetic perturbations enable systematic large-scale, even genome-scale, evaluation of gene function. Pooled screens introduce genetic perturbations into a cell population through viral transduction such that each cell integrates into its DNA a single or small number of library perturbations with barcodes identifying the perturbations. One then selects and physically isolates the subset of cells that exhibit the phenotype of interest. Sequencing the barcodes in the hit cells reveals which genes favored or inhibited the hit phenotype...
January 16, 2018: Current Protocols in Molecular Biology
https://www.readbyqxmd.com/read/29337371/avian-retrovirus-mediated-tumor-specific-gene-knockout
#16
Wei Wang, Bingning Dong, Feng Yang
The RCAS (replication-competent avian sarcoma leukosis virus long-terminal repeat with splice acceptor)-TVA (tumor virus A) gene delivery system has been successfully used in modeling human cancers. Based on this, we have recently developed a novel RCI-Oncogene (RCAS-Cre-IRES-Oncogene) gene delivery system that can be used to efficiently manipulate gene expression in spontaneous tumors in vivo. We used this system for tumor gene knockout (TuKO) and demonstrated a crucial role of FGFR1 in driving mammary tumor metastasis...
January 16, 2018: Current Protocols in Molecular Biology
https://www.readbyqxmd.com/read/29337327/modulating-inflammation-in-a-cutaneous-chronic-wound-model-by-il-10-released-from-collagen-silica-nanocomposites-via-gene-delivery
#17
Xiaolin Wang, Thibaud Coradin, Christophe Hélary
Cutaneous chronic wounds remain a major clinical challenge which requires the development of novel wound dressings. Previously, we showed that collagen-silica nanocomposites consisting of polyethyleneimine (PEI)-DNA complexes associated with silica nanoparticles (SiNP), collagen hydrogel and 3T3 fibroblasts, can work as a local "cell factory". Indeed, the "in-gel" transfection leads to a sustained production and release of biomolecules. Herein, we further explored the possibility for nanocomposites to deliver interleukin-10 (IL-10), a potent anti-inflammatory cytokine, which favors tissue repair...
January 16, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29336355/evaluation-of-cardiolipin-nanodisks-as-lipidreplacement-therapy-for-barth-syndrome
#18
Nikita Ikon, Fong-Fu Hsu, Jennifer Shearer, Trudy M Forte, Robert O Ryan
Barth syndrome (BTHS) is a mitochondrial disorder characterizedby cardiomyopathy and skeletal muscle weakness. Disease results frommutations in the tafazzin (TAZ)gene, encoding a phospholipid transacylase. Defective tafazzin activityresults in an aberrant cardiolipin (CL) profile. The feasibility ofrestoring the intracellular CL profile was tested by in vivo administration of exogenous CL innanodisk (ND) delivery particles. Ninety mg/kg CL (as ND) was administeredto doxycycline-inducible taz shRNAknockdown (KD) mice once a week...
November 1, 2017: Journal of Biomedical Research
https://www.readbyqxmd.com/read/29336241/generation-of-the-fluorescent-hpv16-e7-protein-for-detection-of-delivery-in-vitro
#19
Sepideh Shahbazi, Azam Bolhassani, Arash Arashkia, Esmaeil Sadroddiny
Immunotherapies targeting the human papillomavirus (HPV) oncogenic proteins, E6 and E7, are effective to treat HPV-associated cervical malignancies. In this study, two types of the fusion E7-GFP proteins (i.e., with or without linker) were expressed in different E. coli strains. Then, the efficiency of GFP and E7-GFP transfection was compared with FITC-antibody protein control using TurboFect reagent in the HEK-293T cell line. Our data indicated that both E7-GFP fusion proteins were efficiently produced in M15 E...
January 15, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29335799/precisely-defined-polymers-for-efficient-gene-delivery
#20
REVIEW
Dongsheng He, Hao Lin, Yinglan Yu, Lei Shi, Jiasheng Tu
Gene therapy requires successful delivery of therapeutic nucleic acids into target cells; thus, efficient and safe gene delivery carriers are crucial to its success. Although many polymeric materials have shown their potential as effective nucleic acid carriers, the inherent heterogeneity and polydispersity of these polymers hinder their application in clinical studies because of difficulties in their further precise modification, structure-activity relationship study, as well as consistent manufacturing. Therefore, precisely defined polymers, with potential for site-specific optimization according to structure-activity relationship information and highly controllable production, have been extensively investigated...
January 15, 2018: Topics in Current Chemistry (Journal)
keyword
keyword
89145
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"