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https://www.readbyqxmd.com/read/28944824/efficient-gene-therapy-with-a-combination-of-ultrasound%C3%A2-targeted-microbubble-destruction-and-pei-dna-nls-complexes
#1
Yi-Jia Wang, Qing Zhou, Sheng Cao, Bo Hu, Qing Deng, Nan Jiang, Jingjing Cui
Current strategies of gene transfection are not efficient at achieving a notable therapeutic effect. The aim of the present study was to combine ultrasound‑targeted microbubble destruction (UTMD) with a polyethylenimine/pEGFP‑N3 plasmid/nuclear localization sequence (PEI/DNA/NLS) complex gene delivery system, and evaluate the transfection efficiency of enhanced green fluorescent protein (EGFP) gene delivery to 293T cells using this system. The formation of PEI/DNA/NLS complexes and the protective effects of PEI/NLS were verified by gel electrophoresis...
September 18, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944740/a-novel-nonviral-gene-delivery-system-for-treating-leber-s-congenital-amaurosis
#2
Da Sun, Rebecca M Schur, Zheng-Rong Lu
No abstract text is available yet for this article.
October 2017: Therapeutic Delivery
https://www.readbyqxmd.com/read/28944654/ovarian-cancer-therapy-by-vsvmp-gene-mediated-by-a-paclitaxel-enhanced-nanoparticle
#3
Jianlin Long, Yuping Yang, Tianyi Kang, Wei Zhao, Hao Cheng, Yujiao Wu, Ting Du, Beibei Liu, Yang Li, Feng Luo, Maling Gou
Nanoparticles have great promise for gene delivery. However, the transfection efficiency of nanoparticle-based gene delivery systems is always unsatisfied to meet the requirement of effective gene therapy. Herein, we used low dosage paclitaxel to enhance a nanoscaled gene delivery system that was self-assembled from DOTAP and MPEG-PLA(DPP), creating a paclitaxel-encapsulated DPP nanoparticle (P-DPP). The encapsulated low-dosage paclitaxel significantly improved the gene delivery efficiency of the DPP nanoparticles against multiple cancer cells, in some of which the transfection efficiency is as high as 92%...
September 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28944387/synthetic-polypeptides-from-polymer-design-to-supramolecular-assembly-and-biomedical-application
#4
REVIEW
Ziyuan Song, Zhiyuan Han, Shixian Lv, Chongyi Chen, Li Chen, Lichen Yin, Jianjun Cheng
Synthetic polypeptides from the ring-opening polymerization of N-carboxyanhydrides (NCAs) are one of the most important biomaterials. The unique features of these synthetic polypeptides, including their chemical diversity of side chains and their ability to form secondary structures, enable their broad applications in the field of gene delivery, drug delivery, bio-imaging, tissue engineering, and antimicrobials. In this review article, we summarize the recent advances in the design of polypeptide-based supramolecular structures, including complexes with nucleic acids, micelles, vesicles, hybrid nanoparticles, and hydrogels...
September 25, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28944281/adenovirus-mediated-gene-delivery-potential-applications-for-gene-and-cell-based-therapies-in-the-new-era-of-personalized-medicine
#5
Cody S Lee, Elliot S Bishop, Ruyi Zhang, Xinyi Yu, Evan M Farina, Shujuan Yan, Chen Zhao, Zongyue Zheng, Yi Shu, Xingye Wu, Jiayan Lei, Yasha Li, Wenwen Zhang, Chao Yang, Ke Wu, Ying Wu, Sherwin Ho, Aravind Athiviraham, Michael J Lee, Jennifer Moriatis Wolf, Russell R Reid, Tong-Chuan He
With rapid advances in understanding molecular pathogenesis of human diseases in the era of genome sciences and systems biology, it is anticipated that increasing numbers of therapeutic genes or targets will become available for targeted therapies. Despite numerous setbacks, efficacious gene and/or cell-based therapies still hold the great promise to revolutionize the clinical management of human diseases. It is wildly recognized that poor gene delivery is the limiting factor for most in vivo gene therapies...
June 2017: Genes & Diseases
https://www.readbyqxmd.com/read/28943866/from-the-surface-to-the-deep-sea-bacterial-distributions-across-polymetallic-nodule-fields-in-the-clarion-clipperton-zone-of-the-pacific-ocean
#6
Markus V Lindh, Brianne M Maillot, Christine N Shulse, Andrew J Gooday, Diva J Amon, Craig R Smith, Matthew J Church
Marine bacteria regulate fluxes of matter and energy essential for pelagic and benthic organisms and may also be involved in the formation and maintenance of commercially valuable abyssal polymetallic nodules. Future mining of these nodule fields is predicted to have substantial effects on biodiversity and physicochemical conditions in mined areas. Yet, the identity and distributions of bacterial populations in deep-sea sediments and associated polymetallic nodules has received relatively little attention. We examined bacterial communities using high-throughput sequencing of bacterial 16S rRNA gene fragments from samples collected in the water column, sediment, and polymetallic nodules in the Pacific Ocean (bottom depth ≥4,000 m) in the eastern Clarion-Clipperton Zone...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28943836/evaluating-efficiencies-of-dual-aav-approaches-for-retinal-targeting
#7
Livia S Carvalho, Heikki T Turunen, Sarah J Wassmer, María V Luna-Velez, Ru Xiao, Jean Bennett, Luk H Vandenberghe
Retinal gene therapy has come a long way in the last few decades and the development and improvement of new gene delivery technologies has been exponential. The recent promising results from the first clinical trials for inherited retinal degeneration due to mutations in RPE65 have provided a major breakthrough in the field and have helped cement the use of recombinant adeno-associated viruses (AAV) as the major tool for retinal gene supplementation. One of the key problems of AAV however, is its limited capacity for packaging genomic information to a maximum of around 4...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28943206/kaolinite-in-pharmaceutics-and-biomedicine
#8
REVIEW
Mahmoud E Awad, Alberto López-Galindo, Massimo Setti, Mahmoud M El-Rahmany, César Viseras Iborra
Kaolinite Al2Si2O5(OH)4 is an abundant and inexpensive geomaterial regarded as one of the most common clay minerals in the earth's crust and the most widespread phase among the other kaolin polymorphs (halloysite, dickite and nacrite). Structurally, it is a hydrous aluminum phyllosilicate member belonging to the dioctahedral 1:1 kaolin mineral group. The particle size of the pseudohexagonal kaolinite platelets is normally <2μm (if compared to a human red blood cell of a typical diameter 6.2-8.2μm or to a virus particle of about 50nm diameter)...
September 21, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28941992/nano-delivery-system-targeting-to-cancer-stem-cell-cluster-of-differentiation-biomarkers
#9
REVIEW
Ahad Mokhtarzadeh, Soodabeh Hassanpour, Zahra Farajzadeh Vahid, Maryam Hejazi, Maryam Hashemi, Javad Ranjbari, Maryam Tabarzad, Saeed Noorolyai, Miguel de la Guardia
Cancer stem cells (CSCs) are one of the most important origins of cancer progression and metastasis. CSCs have unique self-renewal properties and diverse cell membrane receptors that induced the resistance to the conventional chemotherapeutic agents. Therefore, the therapeutic removal of CSCs could result in the cancer cure with lack of recurrence and metastasis. In this regard, targeting CSCs in accordance to their specific biomarkers is a talented attitude in cancer therapy. Various CSCs surface biomarkers have been described, which some of them exhibited similarities on different cancer cell types, while the others are cancer specific and have just been reported on one or a few types of cancers...
September 20, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28941965/gestational-respiratory-infections-interacting-with-offspring-hla-and-ctla-4-modifies-incident-%C3%AE-cell-autoantibodies
#10
Kristian F Lynch, Hye-Seung Lee, Carina Törn, Kendra Vehik, Jeffrey P Krischer, Helena Elding Larsson, Michael J Haller, William A Hagopian, Marian J Rewers, Jin-Xiong She, Olli G Simell, Jorma Toppari, Anette-G Ziegler, Beena Akolkar, Heikki Hyöty, Ezio Bonifacio, Åke Lernmark
β-cell autoantibodies against insulin (IAA), GAD65 (GADA) and IA-2 (IA-2A) precede onset of childhood type 1 diabetes (T1D). Incidence of the first appearing β-cell autoantibodies peaks at a young age and is patterned by T1D-associated genes, suggesting an early environmental influence. Here, we tested if gestational infections and interactions with child's human leukocyte antigen (HLA) and non-HLA genes affected the appearance of the first β-cell autoantibody. Singletons of mothers without diabetes (n = 7472) with T1D-associated HLA-DR-DQ genotypes were prospectively followed quarterly through the first 4 years of life, then semiannually until age 6 years, using standardized autoantibody analyses...
September 20, 2017: Journal of Autoimmunity
https://www.readbyqxmd.com/read/28941260/glial-fibrillary-acidic-protein-promoter-determines-transgene-expression-in-satellite-glial-cells-following-intraganglionic-adeno-associated-virus-delivery-in-adult-rats
#11
Hongfei Xiang, Hao Xu, Fan Fan, Seung-Min Shin, Quinn H Hogan, Hongwei Yu
Recombinant adeno-associated viral (AAV)-mediated therapeutic gene transfer to dorsal root ganglia (DRG) is an effective and safe tool for treating chronic pain. However, AAV with various constitutively active promoters leads to transgene expression predominantly to neurons, while glial cells are refractory to AAV transduction in the peripheral nervous system. The present study evaluated whether in vivo satellite glial cell (SGC) transduction in the DRG can be enhanced by the SGC-specific GFAP promoter and by using shH10 and shH19, which are engineered capsid variants with Müller glia-prone transduction...
September 23, 2017: Journal of Neuroscience Research
https://www.readbyqxmd.com/read/28941203/co-delivery-of-drugs-and-genes-using-polymeric-nanoparticles-for-synergistic-cancer-therapeutic-effects
#12
REVIEW
Yi Li, Thavasyappan Thambi, Doo Sung Lee
Drug and gene delivery systems based on nanoparticles, microparticles and hydrogels have been widely studied for cancer treatment in the past decade. To achieve an efficient and safe delivery, selection of drug and gene delivery carrier is critical. Biocompatible polymeric nanoparticles are considerably promising carrier candidates in delivery of drugs and genes because of their unique chemical and physical properties. However, delivery of a drug or gene sometimes cannot achieve a satisfactory treatment effect...
September 22, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28940796/gene-activated-tissue-grafts-for-sustained-bone-morphogenetic-protein-2-delivery-and-bone-engineering-is-muscle-with-fascia-superior-to-muscle-and-fat
#13
Bin Ren, Volker M Betz, Christian Thirion, Michael Salomon, Volkmar Jansson, Peter E Müller, Oliver B Betz
BACKGROUND: Previously, we have presented an expedited strategy for sustained delivery of BMP-2 to bone lesions based on the implantation of gene activated fat and muscle fragments. The aim of the present in vitro experiments was to evaluate the potential of muscle with fascia as a BMP-2 delivering osteo-regenerative implant in comparison to fat tissue and muscle alone. METHODS: Subcutaneous fat, muscle and muscle with fascia were harvested from Fischer 344 rats...
September 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28938577/mitochondria-chaperone-grp75-moonlighting-as-a-cell-cycle-controller-to-derail-endocytosis-provides-an-opportunity-for-nanomicrosphere-intracellular-delivery
#14
Zhihui Gao, Xiuran Niu, Qing Zhang, Hang Chen, Aiai Gao, Shanshan Qi, Rong Xiang, Mattias Belting, Sihe Zhang
Understanding how cancer cells regulate endocytosis during the cell cycle could lead us to capitalize this event pharmacologically. Although certain endocytosis pathways are attenuated during mitosis, the endocytosis shift and regulation during the cell cycle have not been well clarified. The conventional concept of glucose-regulated proteins (GRPs) as protein folding chaperones was updated by discoveries that translocated GRPs assume moonlighting functions that modify the immune response, regulate viral release, and control intracellular trafficking...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938558/human-menstrual-blood-derived-mesenchymal-stem-cells-as-a-cellular-vehicle-for-malignant-glioma-gene-therapy
#15
Xiao-Jun Wang, Bing-Yu Xiang, Ya-Hui Ding, Lu Chen, Hai Zou, Xiao-Zhou Mou, Charlie Xiang
Despite many advances in conventional treatment strategies, there is no effective treatment modality for malignant gliomas. Gene therapy may offer a promising option for gliomas and several gene therapy approaches have shown anti-tumor efficiency in previous studies. Mesenchymal stem cell-based gene therapies, in which stem cells are genetically engineered to express therapeutic molecules, have shown tremendous potential because of their innate homing ability. In this study, human menstrual blood-derived MSCs (MenSC), a novel type of multipotential MSCs displays tropism for human malignant glioma when used as a gene delivery vehicle for therapeutics...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938476/fetal-and-maternal-genetic-variants-influencing-neonatal-vitamin-d-status
#16
Ketil Størdal, Karl Mårild, German Tapia, Margareta Haugen, Arieh S Cohen, Benedicte A Lie, Lars C Stene
Objective: Several genetic polymorphisms determine vitamin D status. We aimed to estimate the strength of association of established 25-hydroyxvitamin D (25OHD) associated variants in the mother and in the fetus, with 25OHD concentration in newborn umbilical cord plasma. Methods: We randomly selected 578 mother and child dyads from the prospective Norwegian Mother and Child Cohort study (MoBa). 25OHD was assayed in maternal samples taken shortly after delivery and in cord samples...
August 18, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28938471/low-vitamin-b12-in-pregnancy-is-associated-with-adipose-derived-circulating-mirs-targeting-ppar%C3%AE-and-insulin-resistance
#17
Antonysunil Adaikalakoteswari, Manu Vatish, Mohammad Tauqeer Alam, Sascha Ott, Sudhesh Kumar, Ponnusamy Saravanan
Context: Low vitamin B12 (B12) during pregnancy is associated with higher maternal obesity, insulin resistance(IR) and gestational diabetes(GDM). B12 is a key co-factor in 1-carbon metabolism. Objective: We hypothesize that B12 plays a role in epigenetic regulation by altering circulating miRNAs(miRs) during adipocyte differentiation and results in an adverse metabolic phenotype. Design, settings and main-outcome measure: Human pre-adipocyte cell-line(Chub-S7) were differentiated in various B12 concentrations: Control(500nM), LowB12(0...
September 5, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28938010/epidermal-growth-factor-receptor-targeted-sonoporation-with-microbubbles-enhances-therapeutic-efficacy-in-a-squamous-cell-carcinoma-model
#18
Fumika Hirabayashi, Kenjiro Iwanaga, Toshinori Okinaga, Osamu Takahashi, Wataru Ariyoshi, Ryo Suzuki, Mutsumi Sugii, Kazuo Maruyama, Kazuhiro Tominaga, Tatsuji Nishihara
Sonoporation is a drug and gene delivery system using ultrasonication that allows the intracellular delivery of foreign molecules that cannot enter cells under normal conditions. We previously reported that sonoporation with microbubbles (MBs) could achieve effective intracellular drug delivery to human gingival squamous carcinoma Ca9-22 cells. In this study, we developed anti-epidermal growth factor receptor (EGFR) antibody-conjugated MBs (EGFR-MBs) and evaluated their capacity to enhance anti-cancer drug toxicity in vitro and in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/28937950/vectors-and-gene-delivery-to-the-retina
#19
Arthur Planul, Deniz Dalkara
One of the great advantages of the retina as a target tissue for gene delivery is the wide array of genetic tools that have been developed in the past decade. This includes a variety of vectors for therapeutic gene delivery to most types of retinal neurons and glia, as well as cell type-specific promoters for restricted gene expression in distinct neuronal subtypes. Within the scope of neuroscience applications and for gene therapy, it is now routine to express reporter genes, replacement genes, neuronal activity indicators, and microbial opsins in specific neuronal types in the mouse retina...
September 15, 2017: Annual Review of Vision Science
https://www.readbyqxmd.com/read/28937681/primary-t-cells-for-mrna-mediated-immunotoxin-delivery
#20
R Eggers, A Philippi, M O Altmeyer, F Breinig, M J Schmitt
Immune cells become increasingly attractive as delivery system for immunotoxins in cancer therapy in order to reduce the intrinsic toxicity and severe side effects of chimeric protein toxins. In this study, we investigated the potential of human primary T cells to deliver a secreted immunotoxin through transient mRNA transfection. The chimeric protein toxin was directed towards the neovasculature of cancer cells by fusing a truncated version of Pseudomonas exotoxin A (PE38) to human vascular endothelial growth factor (VEGF) and to the single chain variable fragment (scFv) of anti-Her2/neu...
September 22, 2017: Gene Therapy
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