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actinobacillus pleuropneumoniae

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https://www.readbyqxmd.com/read/29145759/isolation-and-molecular-characterization-of-a-urease-negative-actinobacillus-pleuropneumoniae-mutant
#1
Hiroya Ito, Sayaka Takahashi, Tetsuo Asai, Yutaka Tamura, Koshi Yamamoto
An atypical urease-negative mutant of Actinobacillus pleuropneumoniae serovar 2 was isolated in Japan. Nucleotide sequence analysis of the urease gene cluster revealed that the insertion of a short DNA sequence into the cbiM gene was responsible for the urease-negative activity of the mutant. Veterinary diagnostic laboratories should be watchful for the presence of aberrant urease-negative A. pleuropneumoniae isolates.
November 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29139136/tilmicosin-and-florfenicol-loaded-hydrogenated-castor-oil-solid-lipid-nanoparticles-to-pigs-combined-antibacterial-activities-and-pharmacokinetics
#2
Z Ling, L Yonghong, L Junfeng, Z Li, L Xianqiang
The combined antibacterial effects of tilmicosin (TIL) and florfenicol (FF) against Actinobacillus pleuropneumoniae (APP) (n = 2), Streptococcus suis (S. suis) (n = 2), and Haemophilus parasuis (HPS) (n = 2) were evaluated by chekerboard test and time-kill assays. The pharmacokinetics (PKs) of TIL- and FF-loaded hydrogenated castor oil (HCO)-solid lipid nanoparticles (SLN) were performed in healthy pigs. The results indicated that TIL and FF showed synergistic or additive antibacterial activities against APP, S...
November 15, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/29126442/effect-of-enriched-housing-on-levels-of-natural-auto-antibodies-in-pigs-co-infected-with-porcine-reproductive-and-respiratory-syndrome-virus-prrsv-and-actinobacillus-pleuropneumoniae
#3
Lu Luo, Ingrid Daniëlle Ellen van Dixhoorn, Inonge Reimert, Bas Kemp, Jantina Elizabeth Bolhuis, Hendrik Karel Parmentier
Housing of pigs in barren, stimulus-poor housing conditions may influence their immune status, including antibody responses to (auto-)antigens, and thus affect immune protection, which will influence the onset and outcome of infection. In the present study, we investigated the effects of environmental enrichment versus barren housing on the level of natural (auto-)antibodies (NA(A)b) and their isotypes (IgM and IgG) binding keyhole limpet hemocyanin (KLH), myelin basic protein (MBP), and phosphorycholine conjugated to bovine serum albumin (PC-BSA) in pigs co-infected with porcine reproductive and respiratory syndrome virus (PRRSV) and Actinobacillus pleuropneumoniae (A...
November 10, 2017: Veterinary Research
https://www.readbyqxmd.com/read/29122004/identification-and-characterization-of-serovar-independent-immunogens-in-actinobacillus-pleuropneumoniae
#4
Fabio Antenucci, Cyrielle Fougeroux, Janine T Bossé, Zofia Magnowska, Camille Roesch, Paul Langford, Peter Johannes Holst, Anders Miki Bojesen
Despite numerous actions to prevent disease, Actinobacillus pleuropneumoniae (A. pleuropneumoniae) remains a major cause of porcine pleuropneumonia, resulting in economic losses to the swine industry worldwide. In this paper, we describe the utilization of a reverse vaccinology approach for the selection and in vitro testing of serovar-independent A. pleuropneumoniae immunogens. Potential immunogens were identified in the complete genomes of three A. pleuropneumoniae strains belonging to different serovars using the following parameters: predicted outer-membrane subcellular localization; ≤ 1 trans-membrane helices; presence of a signal peptide in the protein sequence; presence in all known A...
November 9, 2017: Veterinary Research
https://www.readbyqxmd.com/read/29110751/actinobacillus-pleuropneumoniae-biofilms-role-in-pathogenicity-and-potential-impact-for-vaccination-development
#5
Skander Hathroubi, Abraham Loera-Muro, Alma L Guerrero-Barrera, Yannick D N Tremblay, Mario Jacques
Actinobacillus pleuropneumoniae is a Gram-negative bacterium that belongs to the family Pasteurellaceae. It is the causative agent of porcine pleuropneumonia, a highly contagious respiratory disease that is responsible for major economic losses in the global pork industry. The disease may present itself as a chronic or an acute infection characterized by severe pathology, including hemorrhage, fibrinous and necrotic lung lesions, and, in the worst cases, rapid death. A. pleuropneumoniae is transmitted via aerosol route, direct contact with infected pigs, and by the farm environment...
November 7, 2017: Animal Health Research Reviews
https://www.readbyqxmd.com/read/29083117/update-on-actinobacillus-pleuropneumoniae-knowledge-gaps-and-challenges
#6
E L Sassu, J T Bossé, T J Tobias, M Gottschalk, P R Langford, I Hennig-Pauka
Porcine pleuropneumonia, caused by the bacterial porcine respiratory tract pathogen Actinobacillus pleuropneumoniae, leads to high economic losses in affected swine herds in most countries of the world. Pigs affected by peracute and acute disease suffer from severe respiratory distress with high lethality. The agent was first described in 1957 and, since then, knowledge about the pathogen itself, and its interactions with the host, has increased continuously. This is, in part, due to the fact that experimental infections can be studied in the natural host...
October 30, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29070770/application-of-an-enzyme-linked-immunosorbent-assay-for-detection-of-antibodies-to-actinobacillus-pleuropneumoniae-serovar-15-in-pig-sera
#7
Kaho Teshima, Jina Lee, Ho To, Takashi Kamada, Akihiro Tazumi, Haruna Hirano, Minoru Maruyama, Torata Ogawa, Shinya Nagai, Conny Turni, Nobuyuki Tsutsumi
An indirect enzyme-linked immunosorbent assay (ELISA) using lipopolysaccharide extract as antigen was evaluated for detection of antibodies to Actinobacillus pleuropneumoniae serovar 15. The serovar 15 ELISA had a higher sensitivity and specificity than latex agglutination test for 63 and 80 sera from pigs experimentally infected and not infected with A. pleuropneumoniae, respectively. When the serovar 15 ELISA was applied to 454 field sera, high rates of seropositivity were found in pigs from farms infected with A...
October 24, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29032659/in-silico-analysis-of-putative-drug-and-vaccine-target-of-the-metabolic-pathways-of-actinobacillus-pleuropneumoniae-using-subtractive-comparative-genomics-approach
#8
Biruk Tesfaye Birhanu, Seung-Jin Lee, Na-Hye Park, Ju-Beom Song, Seung-Chun Park
Actinobacillus pleuropneumoniae is a gram-negative bacterium residing in the respiratory tract of pigs that causes porcine respiratory disease complex, which leads to significant losses in the pig industry worldwide. The incidence of drug resistance in this bacterium is increasing; thus, identifying new protein/gene targets for drug and vaccine development is critical. In this study, we used an in silico approach, utilizing several databases, including Kyoto Encyclopedia of Genes and Genomes (KEGG), the database of essential genes (DEG), DrugBank, and Swiss-Prot, to identify non-homologous essential genes and prioritize these proteins for their druggability...
October 13, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29029160/characterization-of-the-actinobacillus-pleuropneumoniae-sxt-related-integrative-and-conjugative-element-iceapl2-and-analysis-of-the-encoded-flor-protein-hydrophobic-residues-in-transmembrane-domains-contribute-dynamically-to-florfenicol-and-chloramphenicol
#9
Yinghui Li, Yanwen Li, Roberto Fernandez Crespo, Leon G Leanse, Paul R Langford, Janine T Bossé
Objectives: To characterize ICEApl2, an SXT-related integrative and conjugative element (ICE) found in a clinical isolate of the porcine pathogen Actinobacillus pleuropneumoniae, and analyse the functional nature of the encoded FloR. Methods: ICEApl2 was identified in the genome of A. pleuropneumoniae MIDG3553. Functional analysis was done using conjugal transfer experiments. MIDG3553 was tested for susceptibility to the antimicrobials for which resistance genes are present in ICEApl2...
October 6, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29028843/efficiency-of-different-air-filter-types-for-pig-facilities-at-laboratory-scale
#10
Cindy Wenke, Janina Pospiech, Tobias Reutter, Uwe Truyen, Stephanie Speck
Air filtration has been shown to be efficient in reducing pathogen burden in circulating air. We determined at laboratory scale the retention efficiency of different air filter types either composed of a prefilter (EU class G4) and a secondary fiberglass filter (EU class F9) or consisting of a filter mat (EU class M6 and F8-9). Four filter prototypes were tested for their capability to remove aerosol containing equine arteritis virus (EAV), porcine reproductive and respiratory syndrome virus (PRRSV), bovine enterovirus 1 (BEV), Actinobacillus pleuropneumoniae (APP), and Staphylococcus (S...
2017: PloS One
https://www.readbyqxmd.com/read/28935749/complete-genome-sequence-of-actinobacillus-pleuropneumoniae-strain-kl-16-serotype-1
#11
Byung-Sun Park, Jemin Han, Dong-Ju Shin, Young-Ju Jeong, Nakhyung Lee
Actinobacillus pleuropneumoniae is a bacterial pathogen causing highly contagious porcine pleuropneumonia. Due to limited information on this species, it is difficult to study the biology of A. pleuropneumoniae at the genome level. Here, we report the fully annotated genome sequence of A. pleuropneumoniae strain KL 16.
September 21, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28929313/an-anti-propionibacterium-acnes-antibody-shows-heterologous-resistance-to-an-actinobacillus-pleuropneumoniae-infection-independent-of-neutrophils-in-mice
#12
Jian-Fang Liu, Qiu-Yue Ma, Ri-Ning Zhu, Meng-Jun Cheng, Chun-Tong Bao, Jing-Min Gu, Chang-Jiang Sun, Paul Richard Langford, Wen-Yu Han, Lian-Cheng Lei
Porcine contagious pleuropneumonia is a highly fatal respiratory disease that is caused by Actinobacillus pleuropneumoniae (APP) and results in tremendous economic losses for the pig breeding industry worldwide. Previous studies have demonstrated that Propionibacterium acnes (PA) could effectively prevent APP infection in mice and pigs. The humoral immune response played a primary role during this process and anti-PA antibody could mediate macrophages to kill the bacteria. However, the role of neutrophils in this process is currently unknown...
September 20, 2017: Immunologic Research
https://www.readbyqxmd.com/read/28899359/transcript-profiling-of-the-immunological-interactions-between-actinobacillus-pleuropneumoniae-serotype-7-and-the-host-by-dual-rna-seq
#13
Ping Li, Zhiwen Xu, Xiangang Sun, Yue Yin, Yi Fan, Jun Zhao, Xiyu Mao, Jianbo Huang, Fan Yang, Ling Zhu
BACKGROUND: The complexity of the pathogenic mechanism underlying the host immune response to Actinobacillus pleuropneumonia (App) makes the use of preventive measures difficult, and a more global view of the host-pathogen interactions and new insights into this process are urgently needed to reveal the pathogenic and immune mechanisms underlying App infection. Here, we infected specific pathogen-free Mus musculus with App serotype 7 by intranasal inoculation to construct an acute hemorrhagic pneumonia infection model and isolated the infected lungs for analysis of the interactions by dual RNA-seq...
September 12, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28886933/characterization-of-actinobacillus-pleuropneumoniae-field-strains-antigenically-related-to-the-3-6-8-15-group-from-diseased-pigs-in-japan-and-argentina
#14
Ho To, Kaho Teshima, Shinya Nagai, Gustavo C Zielinski, Tomohiro Koyama, Jina Lee, Fernando A Bessone, Tetsuji Nagano, Atsushi Oshima, Nobuyuki Tsutsumi
The objectives of this study were to determine the serovar of a collection of Actinobacillus pleuropneumoniae strains within the 3-6-8-15 cross-reacting group and to analyze their phenotypic and genetic properties. Based on the serological tests, forty-seven field strains of Actinobacillus pleuropneumoniae isolated from lungs with pleuropneumonia lesions in Japan and Argentina were found to be serovars belonging to the 3-6-8-15 cross-reacting group. By using a capsule loci-based PCR, twenty-nine (96.7%) and one (3...
September 5, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28852568/etiology-of-acute-respiratory-disease-in-fattening-pigs-in-finland
#15
Minna Haimi-Hakala, Outi Hälli, Tapio Laurila, Mirja Raunio-Saarnisto, Tiina Nokireki, Taina Laine, Suvi Nykäsenoja, Kirsti Pelkola, Joaquim Segales, Marina Sibila, Claudio Oliviero, Olli Peltoniemi, Sinikka Pelkonen, Mari Heinonen
BACKGROUND: The objective of our study was to clinically and etiologically investigate acute outbreaks of respiratory disease in Finland. Our study also aimed to evaluate the clinical use of various methods in diagnosing respiratory infections under field conditions and to describe the antimicrobial resistance profile of the main bacterial pathogen(s) found during the study. METHODS: A total of 20 case herds having finishing pigs showing acute respiratory symptoms and eight control herds showing no clinical signs suggesting of respiratory problems were enrolled in the study...
2017: Porcine Health Management
https://www.readbyqxmd.com/read/28828973/be-aware-of-immunogenic-but-not-protective-antigens-the-actinobacillus-pleuropneumoniae-pala-as-an-example
#16
Caihong Liu, Yurou Cao, Jihong Yang, Haobin Zhao, Kashif Rafiq Zahid, Jin Zhao, Chao Qi, Jinlin Liu
Identification of immunogenic antigens is an important step for the vaccine improvement of infectious diseases. Previous studies indicated that Actinobacillus pleuropneumoniae PalA is homologous to a Haemophilus influenzae protective antigen Hi-PAL (P6) protein. However, PalA protein adversely affects the Apx toxin-based subunit vaccine. In this study, we confirmed that recombinant PalA is immunogenic in animals. Mice immunized with bacterin and PalA (BacPal) showed significantly less antibodies against Apx toxins, relative to that of animals immunized with bacterin alone (Bac) before challenge...
August 22, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28758624/tolc2-is-required-for-the-resistance-colonization-and-virulence-of-actinobacillus-pleuropneumoniae
#17
Ying Li, Sanjie Cao, Luhua Zhang, Jianlin Yuan, Yusheng Yang, Zhuang Zhu, Yiping Wen, Rui Wu, Qin Zhao, Xiaobo Huang, Qigui Yan, Yong Huang, Xiaoping Ma, Xintian Wen
PURPOSE: To colonize and cause infection in the host, pathogens must be well equipped to respond to and survive in several hostile conditions. TolC, an outer membrane channel component used by multidrug efflux pumps and type I secretion systems, is considered to be largely involved in bacterial physiology and virulence. In this study, we attempted to investigate the possible roles of TolC2, a homologue of TolC, in the pathogenesis of Actinobacillus pleuropneumoniae. METHODOLOGY: The cell viability was investigated under stress conditions (oxidative, thermal, acid and osmotic)...
July 31, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28733844/polyamine-binding-protein-potd2-is-required-for-stress-tolerance-and-virulence-in-actinobacillus-pleuropneumoniae
#18
Zhuang Zhu, Qin Zhao, Yu Zhao, Fei Zhang, Xintian Wen, Xiaobo Huang, Yiping Wen, Rui Wu, Qigui Yan, Yong Huang, Xiaoping Ma, Xinfeng Han, Sanjie Cao
Actinobacillus pleuropneumoniae is the cause of porcine contagious pleuropneumonia, which is one of the most important respiratory diseases in swine and causes huge economic losses in the swine industry. PotD, a polyamine-binding protein, has been well characterised in many pathogens of humans and animals. In this study, a ΔpotD2 mutant of A. pleuropneumoniae strain MS71 (serovar 1) was constructed successfully by homologous recombination. Growth curves of different strains showed that the growth of the ΔpotD2 mutant was affected greatly in the logarithmic phase compared with that of parental strain...
July 21, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28687931/differential-cellular-immune-response-of-galleria-mellonella-to-actinobacillus-pleuropneumoniae
#19
Luis Andrés Arteaga Blanco, Josicelli Souza Crispim, Kenner Morais Fernandes, Leandro Licursi de Oliveira, Monalessa Fábia Pereira, Denise Mara Soares Bazzolli, Gustavo Ferreira Martins
In the present work, we have investigate the cellular immune response of Galleria mellonella larvae against three strains of the gram-negative bacterium Actinobacillus pleuropneumoniae: low-virulence (780), high-virulence (1022) and the serotype 8 reference strain (R8). Prohemocytes, plasmatocytes, granulocytes, oenocytoids and spherulocytes were distinguished according to their size and morphology, their molecular markers and dye-staining properties and their role in the immune response. Total hemocyte count, differential hemocyte count, lysosome activity, autophagic response, cell viability and caspase-3 activation were determined in circulating hemocytes of naive and infected larvae...
July 8, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28685648/simultaneous-detection-of-antibodies-to-five-actinobacillus-pleuropneumoniae-serovars-using-bead-based-multiplex-analysis
#20
Sanne Schou Berger, Klara Tølbøll Lauritsen, Ulrik Boas, Peter Lind, Lars Ole Andresen
We developed and made a preliminary validation of a bead-based multiplexed immunoassay for simultaneous detection of porcine serum antibodies to Actinobacillus pleuropneumoniae serovars 1, 2, 6, 7, and 12. Magnetic fluorescent beads were coupled with A. pleuropneumoniae antigens and tested with a panel of serum samples from experimentally infected pigs and with serum samples from uninfected and naturally infected pigs. The multiplex assay was compared to in-house ELISAs and complement fixation (CF) tests, which have been used for decades as tools for herd classification in the Danish Specific Pathogen Free system...
November 2017: Journal of Veterinary Diagnostic Investigation
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