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actinobacillus pleuropneumoniae

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https://www.readbyqxmd.com/read/28539918/the-sapa-protein-is-involved-in-resistance-to-antimicrobial-peptide-pr-39-and-virulence-of-actinobacillus-pleuropneumoniae
#1
Fang Xie, Yalei Wang, Gang Li, Shuanghong Liu, Ning Cui, Siguo Liu, Paul R Langford, Chunlai Wang
Antimicrobial peptides are essential to the innate immune defense of the mammal against bacterial infection. However, pathogenic bacteria have evolved multiple strategies to resist and evade antimicrobial peptides, which is vital to bacterial survival and colonization in hosts. PR-39 is a linear porcine antimicrobial peptide containing 39 amino acid residues with a high proline content. Resistance to antimicrobial peptide PR-39 has been observed in Actinobacillus pleuropneumoniae. However, little is known about the factors required for this resistance...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28532791/p518-a-small-flor-plasmid-from-a-south-american-isolate-of-actinobacillus-pleuropneumoniae
#2
Giarlã Cunha da Silva, Ciro César Rossi, Mateus Ferreira Santana, Paul R Langford, Janine T Bossé, Denise Mara Soares Bazzolli
A small (3.9kb) plasmid (p518), conferring resistance to florfenicol (MIC >8μg/mL) and chloramphenicol (MIC >8μg/mL) was isolated from an Actinobacillus pleuropneumoniae clinical isolate from Southeastern Brazil. To date, this is the smallest florfenicol resistance plasmid isolated from a member of the Pasteurellaceae. The complete nucleotide of this plasmid revealed a unique gene arrangement compared to previously reported florfenicol resistance plasmids found in other members of the Pasteurellaceae...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28513418/flagellar-expression-in-clinical-isolates-of-non-typeable-haemophilus-influenzae
#3
Alejandro Carabarin-Lima, Patricia Lozano-Zarain, Miguel Castañeda-Lucio, Claudia Fabiola Martínez de la Peña, Julieta Martinez-Garcia, Norarizbeth Lara Flores, Elías Campos de la Cruz, Sirenia González-Posos, Rosa Del Carmen Rocha-Gracia
PURPOSE: Haemophilus influenzae is a commensal organism found in the upper respiratory tract of humans. When H. influenzae becomes a pathogen, these bacteria can move out of their commensal niche and cause multiple respiratory tract diseases such as otitis media, sinusitis, conjunctivitis and bronchitis in children, and chronic obstructive pulmonary disease in adults. However, H. influenzae is currently considered a non-flagellate bacterium. METHODOLOGY AND RESULTS: In this study, 90 clinical isolates of H...
May 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28496988/randomised-controlled-field-study-to-evaluate-the-efficacy-and-clinical-safety-of-a-single-8%C3%A2-mg-kg-injectable-dose-of-marbofloxacin-compared-with-one-or-two-doses-of-7-5%C3%A2-mg-kg-injectable-enrofloxacin-for-the-treatment-of-actinobacillus-pleuropneumoniae-infections
#4
Erik Grandemange, Pierre-Alexandre Perrin, Dejean Cvejic, Miriam Haas, Tim Rowan, Klaus Hellmann
BACKGROUND: Acute outbreaks of Actinobacillus pleuropneumoniae (APP) require rapid, effective, parenteral antimicrobial treatment. The efficacy and safety of a single, short-acting, high dose of marbofloxacin (Forcyl® swine 160 mg/mL) compared with 1 or 2 doses of 7.5 mg/kg enrofloxacin in APP outbreaks in European farms was studied. METHODS: A controlled, randomised block, blinded, multicentre, field study was conducted on four farms with acute respiratory disease associated with APP...
2017: Porcine Health Management
https://www.readbyqxmd.com/read/28484709/pharmacokinetic-and-pharmacodynamic-evaluation-of-marbofloxacin-in-pig-against-korean-local-isolates-of-actinobacillus-pleuropneumoniae
#5
Md Akil Hossain, Hae-Chul Park, Kyunghun Jeong, Yang Ho Jang, Dae Gyun Kim, JeongWoo Kang, Kwang-Jick Lee
The pharmacokinetics of marbofloxacin in pigs after intravenous (i.v.), intramuscular (i.m.), and peroral (p.o.) administration and pharmacokinetic/pharmacodynamic indices of this drug against Korean local isolates of Actinobacillus pleuropneumoniae were determined in this study. Marbofloxacin (2.50 mg/kg of body weight) was administered, and blood samples were collected with designated time intervals. Plasma-extracted marbofloxacin was injected into the LC-MS/MS system. The in vitro and ex vivo antibacterial activities of marbofloxacin were evaluated against 20 isolates of A...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28448619/pyridoxal-phosphate-synthases-pdxs-pdxt-are-required-for-actinobacillus-pleuropneumoniae-viability-stress-tolerance-and-virulence
#6
Fang Xie, Gang Li, Yalei Wang, Yanhe Zhang, Long Zhou, Chengcheng Wang, Shuanghong Liu, Siguo Liu, Chunlai Wang
Pyridoxal 5'-phosphate (PLP) is an essential cofactor for numerous enzymes involved in a diversity of cellular processes in living organisms. Previous analysis of the Actinobacillus pleuropneumoniae S-8 genome sequence revealed the presence of pdxS and pdxT genes, which are implicated in deoxyxylulose 5-phosphate (DXP)-independent pathway of PLP biosynthesis; however, little is known about their roles in A. pleuropneumoniae pathogenicity. Our data demonstrated that A. pleuropneumoniae could synthesize PLP by PdxS and PdxT enzymes...
2017: PloS One
https://www.readbyqxmd.com/read/28381551/production-of-homogeneous-glycoprotein-with-multi-site-modifications-by-an-engineered-n-glycosyltransferase-mutant
#7
Qitao Song, Zhigang Wu, Yueyuan Fan, Woran Song, Peiru Zhang, Li Wang, Faxing Wang, Yangyang Xu, Peng George Wang, Jiansong Cheng
Naturally occurring N- glycoproteins exhibit glycoform heterogeneity with respect to N-glycan sequon occupancy (macroheterogeneity) and glycan structure (microheterogeneity). However, access to well-defined glycoproteins is always important for both basic research and therapeutic purposes. As a result, there has been substantial effort to identify and understand the catalytic properties of N-glycosyltransferases, enzymes that install the first glycan on the protein chain. In this study, we found that ApNGT, a newly discovered cytoplasmic N- glycosyltransferase from Actinobacillus pleuropneumoniae, has strict selectivity towards the residues around the Asn of N-glycosylation sequon by screening a small library of synthetic peptides...
April 5, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28348142/survey-of-susceptibility-to-marbofloxacin-in-bacteria-isolated-from-diseased-pigs-in-europe
#8
F El Garch, S Kroemer, D Galland, I Morrissey, F Woehrle
A monitoring programme of marbofloxacin susceptibility of bacteria from Europe causing respiratory tract infection and meningitis in pigs has been active since 1994 and 2002, respectively. Monitoring digestive, metritis and urinary tract infection (UTI) in pigs has been active since 2005 and susceptibility results until 2013 are presented. Minimum inhibitory concentration (MIC) was determined by broth microdilution. For MIC interpretation, Vétoquinol-evaluated breakpoints were applied. For digestive pathogens, Escherichia coli and Salmonella species (1717 and 300 isolates, respectively) exhibited 7...
March 27, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28321207/whole-genome-sequencing-for-surveillance-of-antimicrobial-resistance-in-actinobacillus-pleuropneumoniae
#9
Janine T Bossé, Yanwen Li, Jon Rogers, Roberto Fernandez Crespo, Yinghui Li, Roy R Chaudhuri, Matthew T G Holden, Duncan J Maskell, Alexander W Tucker, Brendan W Wren, Andrew N Rycroft, Paul R Langford
The aim of this study was to evaluate the correlation between antimicrobial resistance (AMR) profiles of 96 clinical isolates of Actinobacillus pleuropneumoniae, an important porcine respiratory pathogen, and the identification of AMR genes in whole genome sequence (wgs) data. Susceptibility of the isolates to nine antimicrobial agents (ampicillin, enrofloxacin, erythromycin, florfenicol, sulfisoxazole, tetracycline, tilmicosin, trimethoprim, and tylosin) was determined by agar dilution susceptibility test...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28300777/functional-contributions-of-positive-charges-in-the-pore-lining-helix-3-of-the-bordetella-pertussis-cyaa-hemolysin-to-hemolytic-activity-and-ion-channel-opening
#10
Chattip Kurehong, Chalermpol Kanchanawarin, Busaba Powthongchin, Panchika Prangkio, Gerd Katzenmeier, Chanan Angsuthanasombat
The Bordetella pertussis CyaA-hemolysin (CyaA-Hly) domain was previously demonstrated to be an important determinant for hemolysis against target erythrocytes and ion-channel formation in planar lipid bilayers (PLBs). Here, net-charge variations in the pore-lining helix of thirteen related RTX cytolysins including CyaA-Hly were revealed by amino acid sequence alignments, reflecting their different degrees of hemolytic activity. To analyze possible functional effects of net-charge alterations on hemolytic activity and channel formation of CyaA-Hly, specific mutations were made at Gln(574) or Glu(581) in its pore-lining α3 of which both residues are highly conserved Lys in the three highly active RTX cytolysins (i...
March 16, 2017: Toxins
https://www.readbyqxmd.com/read/28284596/kinetics-of-single-and-dual-infection-of-pigs-with-swine-influenza-virus-and-actinobacillus-pleuropneumoniae
#11
Małgorzata Pomorska-Mól, Arkadiusz Dors, Krzysztof Kwit, Andrzej Kowalczyk, Ewelina Stasiak, Zygmunt Pejsak
Porcine respiratory disease complex (PRDC) is a common problem in modern pork production worldwide. Pathogens that are amongst other pathogens frequently involved in PRDC etiology are swine influenza virus (SIV) and A. pleuropneumoniae. The effect of dual infection with mentioned pathogens has not been investigated to date. The aim of the present study was to evaluate the kinetics of single and dual infection of pigs with SIV and A. pleuropneumoniae with regard to clinical course, pathogens shedding, lung lesions and early immune response...
March 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28245826/host-pathogen-interplay-at-primary-infection-sites-in-pigs-challenged-with-actinobacillus-pleuropneumoniae
#12
Elena L Sassu, Janna Frömbling, J Catharina Duvigneau, Ingrid Miller, Andrea Müllebner, Ana M Gutiérrez, Tom Grunert, Martina Patzl, Armin Saalmüller, Alexandra von Altrock, Anne Menzel, Martin Ganter, Joachim Spergser, Marion Hewicker-Trautwein, Jutta Verspohl, Monika Ehling-Schulz, Isabel Hennig-Pauka
BACKGROUND: Actinobacillus (A.) pleuropneumoniae is the causative agent of porcine pleuropneumonia and causes significant losses in the pig industry worldwide. Early host immune response is crucial for further progression of the disease. A. pleuropneumoniae is either rapidly eliminated by the immune system or switches to a long-term persistent form. To gain insight into the host-pathogen interaction during the early stages of infection, pigs were inoculated intratracheally with A. pleuropneumoniae serotype 2 and humanely euthanized eight hours after infection...
February 28, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28166835/frequency-of-th17-cells-correlates-with-the-presence-of-lung-lesions-in-pigs-chronically-infected-with-actinobacillus-pleuropneumoniae
#13
Elena L Sassu, Andrea Ladinig, Stephanie C Talker, Maria Stadler, Christian Knecht, Heiko Stein, Janna Frömbling, Barbara Richter, Joachim Spergser, Monika Ehling-Schulz, Robert Graage, Isabel Hennig-Pauka, Wilhelm Gerner
Porcine contagious pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) remains one of the major causes of poor growth performance and respiratory disease in pig herds. While the role of antibodies against APP has been intensely studied, the porcine T cell response remains poorly characterized. To address this, pigs were intranasally infected with APP serotype 2 and euthanized during the acute phase [6-10 days post-infection (dpi)] or the chronic phase of APP infection (27-31 dpi). Lymphocytes isolated from blood, tonsils, lung tissue and tracheobronchial lymph nodes were analyzed by intracellular cytokine staining (ICS) for IL-17A, IL-10 and TNF-α production after in vitro stimulation with crude capsular extract (CCE) of the APP inoculation strain...
February 6, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28145487/the-antibacterial-activities-of-aditoprim-and-its-efficacy-in-the-treatment-of-swine-streptococcosis
#14
Guyue Cheng, Yamei Xu, Xudong Zhu, Shuyu Xie, Liye Wang, Lingli Huang, Haihong Hao, Zhenli Liu, Yuanhu Pan, Dongmei Chen, Yulian Wang, Zonghui Yuan
Aditoprim (ADP) has potential use as an antimicrobial agent in animals. However, its pharmacodynamic properties have not been systematically studied yet. In this study, the in vitro antibacterial activities of ADP and its main metabolites were assayed, and the in vivo antibacterial efficacy of ADP for the treatment of swine streptococcosis was evaluated. It was shown that Salmonella and Streptococcus from swine, Escherichia coli and Salmonella from chickens, E. coli, Streptococcus, Mannheimia, Pasteurella from calves, Streptococcus and Mannheimia from sheep, and E...
February 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28113129/factors-influencing-the-potency-of-marbofloxacin-for-pig-pneumonia-pathogens-actinobacillus-pleuropneumoniae-and-pasteurella-multocida
#15
L Dorey, S Hobson, P Lees
For the pig respiratory tract pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida, Minimum Inhibitory Concentration (MIC) of marbofloxacin was determined in recommended broths and pig serum at three inoculum strengths. MICs in both growth matrices increased progressively from low, through medium to high starting inoculum counts, 10(4), 10(6) and 10(8)CFU/mL, respectively. P. multocida MIC ratios for high:low inocula were 14:4:1 for broth and 28.2:1 for serum. Corresponding MIC ratios for A...
April 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28101885/what-is-the-true-in%C3%A2-vitro-potency-of-oxytetracycline-for-the-pig-pneumonia-pathogens-actinobacillus-pleuropneumoniae-and-pasteurella-multocida
#16
L Dorey, S Hobson, P Lees
The pharmacodynamics of oxytetracycline was determined for pig respiratory tract pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida. Indices of potency were determined for the following: (i) two matrices, broth and pig serum; (ii) five overlapping sets of twofold dilutions; and (iii) a high strength starting culture. For A. pleuropneumoniae, minimum inhibitory concentration (MIC) was similar for the two matrices, but for P. multocida, differences were marked and significantly different. MIC and minimum bactericidal concentration (MBC) serum: broth ratios for A...
January 18, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28090673/pharmacokinetic-pharmacodynamic-integration-and-modelling-of-oxytetracycline-for-the-porcine-pneumonia-pathogens-actinobacillus-pleuropneumoniae-and-pasteurella-multocida
#17
L Dorey, L Pelligand, Z Cheng, P Lees
Pharmacokinetic-pharmacodynamic (PK/PD) integration and modelling were used to predict dosage schedules of oxytetracycline for two pig pneumonia pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida. Minimum inhibitory concentration (MIC) and mutant prevention concentration (MPC) were determined in broth and porcine serum. PK/PD integration established ratios of average concentration over 48 h (Cav0-48 h )/MIC of 5.87 and 0.27 μg/mL (P. multocida) and 0.70 and 0.85 μg/mL (A. pleuropneumoniae) for broth and serum MICs, respectively...
January 16, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28077594/the-n-linking-glycosylation-system-from-actinobacillus-pleuropneumoniae-is-required-for-adhesion-and-has-potential-use-in-glycoengineering
#18
Jon Cuccui, Vanessa S Terra, Janine T Bossé, Andreas Naegeli, Sherif Abouelhadid, Yanwen Li, Chia-Wei Lin, Prerna Vohra, Alexander W Tucker, Andrew N Rycroft, Duncan J Maskell, Markus Aebi, Paul R Langford, Brendan W Wren
Actinobacillus pleuropneumoniae is a mucosal respiratory pathogen causing contagious porcine pleuropneumonia. Pathogenesis studies have demonstrated a major role for the capsule, exotoxins and outer membrane proteins. Actinobacillus pleuropneumoniae can also glycosylate proteins, using a cytoplasmic N-linked glycosylating enzyme designated NGT, but its transcriptional arrangement and role in virulence remains unknown. We investigated the NGT locus and demonstrated that the putative transcriptional unit consists of rimO, ngt and a glycosyltransferase termed agt...
January 2017: Open Biology
https://www.readbyqxmd.com/read/28061786/attenuated-actinobacillus-pleuropneumoniae-double-deletion-mutant-s-8%C3%A2-clpp-apxiic-confers-protection-against-homologous-or-heterologous-strain-challenge
#19
RANDOMIZED CONTROLLED TRIAL
Fang Xie, Gang Li, Long Zhou, Yanhe Zhang, Ning Cui, Siguo Liu, Chunlai Wang
BACKGROUND: Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, which leads to large economic losses to the swine industry worldwide. In this study, S-8△clpP△apxIIC, a double-deletion mutant of A. pleuropneumoniae was constructed, and its safety and protective efficacy were evaluated in pigs. RESULTS: The S-8△clpP△apxIIC mutant exhibited attenuated virulence in a murine (BALB/c) model, and caused no detrimental effects on pigs even at a dose of up to 1...
January 6, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28053219/a-unique-capsule-locus-in-the-newly-designated-actinobacillus-pleuropneumoniae-serovar-16-and-development-of-a-diagnostic-pcr-assay
#20
Janine T Bossé, Yanwen Li, Rita Sárközi, Marcelo Gottschalk, Øystein Angen, Katerina Nedbalcova, Andrew N Rycroft, László Fodor, Paul R Langford
Actinobacillus pleuropneumoniae causes pleuropneumonia, an economically significant lung disease of pigs. Recently, isolates of A. pleuropneumoniae that were serologically distinct from the previously characterized 15 serovars were described, and a proposal was put forward that they comprised a new serovar, serovar 16. Here we used whole-genome sequencing of the proposed serovar 16 reference strain A-85/14 to confirm the presence of a unique capsular polysaccharide biosynthetic locus. For molecular diagnostics, primers were designed from the capsule locus of strain A-85/14, and a PCR was formulated that differentiated serovar 16 isolates from all 15 known serovars and other common respiratory pathogenic/commensal bacteria of pigs...
March 2017: Journal of Clinical Microbiology
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