keyword
MENU ▼
Read by QxMD icon Read
search

actinobacillus pleuropneumoniae

keyword
https://www.readbyqxmd.com/read/28733844/polyamine-binding-protein-potd2-is-required-for-stress-tolerance-and-virulence-in-actinobacillus-pleuropneumoniae
#1
Zhuang Zhu, Qin Zhao, Yu Zhao, Fei Zhang, Xintian Wen, Xiaobo Huang, Yiping Wen, Rui Wu, Qigui Yan, Yong Huang, Xiaoping Ma, Xinfeng Han, Sanjie Cao
Actinobacillus pleuropneumoniae is the cause of porcine contagious pleuropneumonia, which is one of the most important respiratory diseases in swine and causes huge economic losses in the swine industry. PotD, a polyamine-binding protein, has been well characterised in many pathogens of humans and animals. In this study, a ΔpotD2 mutant of A. pleuropneumoniae strain MS71 (serovar 1) was constructed successfully by homologous recombination. Growth curves of different strains showed that the growth of the ΔpotD2 mutant was affected greatly in the logarithmic phase compared with that of parental strain...
July 21, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28687931/differential-cellular-immune-response-of-galleria-mellonella-to-actinobacillus-pleuropneumoniae
#2
Luis Andrés Arteaga Blanco, Josicelli Souza Crispim, Kenner Morais Fernandes, Leandro Licursi de Oliveira, Monalessa Fábia Pereira, Denise Mara Soares Bazzolli, Gustavo Ferreira Martins
In the present work, we have investigate the cellular immune response of Galleria mellonella larvae against three strains of the gram-negative bacterium Actinobacillus pleuropneumoniae: low-virulence (780), high-virulence (1022) and the serotype 8 reference strain (R8). Prohemocytes, plasmatocytes, granulocytes, oenocytoids and spherulocytes were distinguished according to their size and morphology, their molecular markers and dye-staining properties and their role in the immune response. Total hemocyte count, differential hemocyte count, lysosome activity, autophagic response, cell viability and caspase-3 activation were determined in circulating hemocytes of naive and infected larvae...
July 8, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28685648/simultaneous-detection-of-antibodies-to-five-actinobacillus-pleuropneumoniae-serovars-using-bead-based-multiplex-analysis
#3
Sanne Schou Berger, Klara Tølbøll Lauritsen, Ulrik Boas, Peter Lind, Lars Ole Andresen
We have developed and made a preliminary validation of a bead-based multiplexed immunoassay for simultaneous detection of porcine serum antibodies to Actinobacillus pleuropneumoniae serovars 1, 2, 6, 7, and 12. Magnetic fluorescent beads were coupled with A. pleuropneumoniae antigens and tested with a panel of serum samples from experimentally infected pigs and with serum samples from uninfected and naturally infected pigs. The multiplex assay was compared to in-house ELISAs and complement fixation (CF) tests, which have been used for decades as tools for herd classification in the Danish Specific Pathogen Free system...
June 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28666426/prediction-of-marbofloxacin-dosage-for-the-pig-pneumonia-pathogens-actinobacillus-pleuropneumoniae-and-pasteurella-multocida-by-pharmacokinetic-pharmacodynamic-modelling
#4
Lucy Dorey, Ludovic Pelligand, Peter Lees
BACKGROUND: Bacterial pneumonia in pigs occurs widely and requires antimicrobial therapy. It is commonly caused by the pathogens Actinobacillus pleuropneumoniae and Pasteurella multocida. Marbofloxacin is an antimicrobial drug of the fluoroquinolone class, licensed for use against these organisms in the pig. In recent years there have been major developments in dosage schedule design, based on integration and modelling of pharmacokinetic (PK) and pharmacodynamic (PD) data, with the objective of optimising efficacy and minimising the emergence of resistance...
July 1, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28652150/biochemical-characterization-of-a-novel-%C3%AE-galactosidase-from-paenibacillus-barengoltzii-suitable-for-lactose-hydrolysis-and-galactooligosaccharides-synthesis
#5
Yu Liu, Zhou Chen, Zhengqiang Jiang, Qiaojuan Yan, Shaoqing Yang
A β-galactosidase gene (PbBGal2A) was cloned from Paenibacillus barengoltzii and expressed in Escherichia coli. The in silico analysis of the deduced amino acid sequences revealed that PbBGal2A shared the highest identity of 40% with the characterized glycoside hydrolase (GH) family 2 β-galactosidase from Actinobacillus pleuropneumoniae. The recombinant β-galactosidase (PbBGal2A) was purified with a molecular mass of 124.2kDa on SDS-PAGE. The optimal pH and temperature of PbBGal2A were determined to be pH 7...
June 24, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28645327/impact-of-growth-matrix-on-pharmacodynamics-of-antimicrobial-drugs-for-pig-pneumonia-pathogens
#6
Lucy Dorey, Peter Lees
BACKGROUND: The most widely used measure of potency of antimicrobial drugs is Minimum Inhibitory Concentration (MIC). MIC is usually determined under standardised conditions in broths formulated to optimise bacterial growth on a species-by-species basis. This ensures comparability of data between laboratories. However, differences in values of MIC may arise between broths of differing chemical composition and for some drug classes major differences occur between broths and biological fluids such as serum and inflammatory exudate...
June 23, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28637172/identification-of-four-type-ii-toxin-antitoxin-systems-in-actinobacillus-pleuropneumoniae
#7
Chengkun Zheng, Xigong Zhao, Ting Zeng, Manman Cao, Jiali Xu, Guolin Shi, Jinquan Li, Huanchun Chen, Weicheng Bei
Toxin-antitoxin systems are small genetic elements that are widely prevalent in the genomes of bacteria and archaea. These modules have been identified in various bacteria and proposed to play an important role in bacterial physiology and virulence. However, their presence in the genomes of Actinobacillus species has received no attention. In this study, we describe the identification of four type II TA systems in Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Reverse transcription PCR analysis revealed that the genes encoding the toxin and antitoxin are co-transcribed...
June 20, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28622856/survey-for-selected-pathogens-in-wild-pigs-sus-scrofa-from-guam-marianna-islands-usa
#8
Christopher A Cleveland, Anthony DeNicola, J P Dubey, Dolores E Hill, Roy D Berghaus, Michael J Yabsley
Pigs (Sus scrofa) were introduced to Guam in the 1600's and are now present in high densities throughout the island. Wild pigs are reservoirs for pathogens of concern to domestic animals and humans. Exposure to porcine parvovirus, transmissible gastroenteritis, and Leptospira interrogans has been documented in domestic swine but data from wild pigs are lacking. The close proximity of humans, domestic animals, and wild pigs, combined with the liberal hunting of wild pigs, results in frequent opportunities for pathogen transmission...
June 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28622855/b-cell-cross-epitope-of-propionibacterium-acnes-and-actinobacillus-pleuropneumonia-selected-by-phage-display-library-can-efficiently-protect-from-actinobacillus-pleuropneumonia-infection
#9
Jianfang Liu, Qiuyue Ma, Feng Yang, Rining Zhu, Jingmin Gu, Changjiang Sun, Xin Feng, Chongtao Du, Paul R Langford, Wenyu Han, Junling Yang, Liancheng Lei
Contagious porcine pleuropneumonia (CPP), caused by Actinobacillus pleuropneumoniae (APP), is a highly transmissible and fatal respiratory illness that causes tremendous economic losses for the pig breeding industry worldwide. Propionibacterium acnes (PA) has a strong cross-reaction with anti-APP1 and anti-APP5 serum and can efficiently prevent APP infection, which was fortuitously found in researching the differential gene between the different APP serotypes. There seems to be some natural cross-protection between PA and APP...
June 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28619145/thymol-kills-bacteria-reduces-biofilm-formation-and-protects-mice-against-a-fatal-infection-of-actinobacillus-pleuropneumoniae-strain-l20
#10
Lei Wang, Xueqin Zhao, Chunling Zhu, Xiaojing Xia, Wanhai Qin, Mei Li, Tongzhao Wang, Shijun Chen, Yanzhao Xu, Bolin Hang, Yawei Sun, Jinqing Jiang, Langford Paul Richard, Liancheng Lei, Gaiping Zhang, Jianhe Hu
Actinobacillus pleuropneumoniae is the causative agent of the highly contagious and deadly respiratory infection porcine pleuropneumonia, resulting in serious losses to the pig industry worldwide. Alternative to antibiotics are urgently needed due to the serious increase in antimicrobial resistance. Thymol is a monoterpene phenol and efficiently kills a variety of bacteria. This study found that thymol has strong bactericidal effects on the A. pleuropneumoniae 5b serotype strain, an epidemic strain in China...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28588496/clinical-efficacy-and-residue-depletion-of-10-enrofloxacin-enteric-coated-granules-in-pigs
#11
Zhixin Lei, Qianying Liu, Bing Yang, Jincheng Xiong, Kun Li, Saeed Ahmed, Liping Hong, Pin Chen, Qigai He, Jiyue Cao
A new, more palatable formulation of 10% enrofloxacin enteric-coated granules was investigated to evaluate the pharmacokinetic effect in plasma, the residue elimination in tissues and the clinical efficacy against Actinobacillus pleuropneumonia (APP) and Mycoplasam suis (MS) in pigs. In this study, the enrofloxacin concentrations in plasma and tissues were detected using high-performance liquid chromatography with phosphate buffer (pH = 3) and acetonitrile. The pharmacokinetics and elimination of enrofloxacin enteric-coated granules were performed after oral administration at a single dose of 10 mg/kg body weight (bw) and 5 mg/kg twice per day for 5 consecutive days, respectively...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28552968/pharmacokinetic-pharmacodynamic-integration-and-modelling-of-florfenicol-for-the-pig-pneumonia-pathogens-actinobacillus-pleuropneumoniae-and-pasteurella-multocida
#12
Lucy Dorey, Ludovic Pelligand, Zhangrui Cheng, Peter Lees
Pharmacokinetic-pharmacodynamic (PK/PD) integration and modelling were used to predict dosage schedules for florfenicol for two pig pneumonia pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida. Pharmacokinetic data were pooled for two bioequivalent products, pioneer and generic formulations, administered intramuscularly to pigs at a dose rate of 15 mg/kg. Antibacterial potency was determined in vitro as minimum inhibitory concentration (MIC) and Mutant Prevention Concentration in broth and pig serum, for six isolates of each organism...
2017: PloS One
https://www.readbyqxmd.com/read/28539918/the-sapa-protein-is-involved-in-resistance-to-antimicrobial-peptide-pr-39-and-virulence-of-actinobacillus-pleuropneumoniae
#13
Fang Xie, Yalei Wang, Gang Li, Shuanghong Liu, Ning Cui, Siguo Liu, Paul R Langford, Chunlai Wang
Antimicrobial peptides are essential to the innate immune defense of the mammal against bacterial infection. However, pathogenic bacteria have evolved multiple strategies to resist and evade antimicrobial peptides, which is vital to bacterial survival and colonization in hosts. PR-39 is a linear porcine antimicrobial peptide containing 39 amino acid residues with a high proline content. Resistance to antimicrobial peptide PR-39 has been observed in Actinobacillus pleuropneumoniae. However, little is known about the factors required for this resistance...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28532791/p518-a-small-flor-plasmid-from-a-south-american-isolate-of-actinobacillus-pleuropneumoniae
#14
Giarlã Cunha da Silva, Ciro César Rossi, Mateus Ferreira Santana, Paul R Langford, Janine T Bossé, Denise Mara Soares Bazzolli
A small (3.9kb) plasmid (p518), conferring resistance to florfenicol (MIC >8μg/mL) and chloramphenicol (MIC >8μg/mL) was isolated from an Actinobacillus pleuropneumoniae clinical isolate from Southeastern Brazil. To date, this is the smallest florfenicol resistance plasmid isolated from a member of the Pasteurellaceae. The complete nucleotide of this plasmid revealed a unique gene arrangement compared to previously reported florfenicol resistance plasmids found in other members of the Pasteurellaceae...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28513418/flagellar-expression-in-clinical-isolates-of-non-typeable-haemophilus-influenzae
#15
Alejandro Carabarin-Lima, Patricia Lozano-Zarain, Miguel Castañeda-Lucio, Claudia Fabiola Martínez de la Peña, Julieta Martinez-Garcia, Norarizbeth Lara Flores, Elías Campos de la Cruz, Sirenia González-Posos, Rosa Del Carmen Rocha-Gracia
PURPOSE: Haemophilus influenzae is a commensal organism found in the upper respiratory tract of humans. When H. influenzae becomes a pathogen, these bacteria can move out of their commensal niche and cause multiple respiratory tract diseases such as otitis media, sinusitis, conjunctivitis and bronchitis in children, and chronic obstructive pulmonary disease in adults. However, H. influenzae is currently considered a non-flagellate bacterium. METHODOLOGY AND RESULTS: In this study, 90 clinical isolates of H...
May 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28496988/randomised-controlled-field-study-to-evaluate-the-efficacy-and-clinical-safety-of-a-single-8%C3%A2-mg-kg-injectable-dose-of-marbofloxacin-compared-with-one-or-two-doses-of-7-5%C3%A2-mg-kg-injectable-enrofloxacin-for-the-treatment-of-actinobacillus-pleuropneumoniae-infections
#16
Erik Grandemange, Pierre-Alexandre Perrin, Dejean Cvejic, Miriam Haas, Tim Rowan, Klaus Hellmann
BACKGROUND: Acute outbreaks of Actinobacillus pleuropneumoniae (APP) require rapid, effective, parenteral antimicrobial treatment. The efficacy and safety of a single, short-acting, high dose of marbofloxacin (Forcyl® swine 160 mg/mL) compared with 1 or 2 doses of 7.5 mg/kg enrofloxacin in APP outbreaks in European farms was studied. METHODS: A controlled, randomised block, blinded, multicentre, field study was conducted on four farms with acute respiratory disease associated with APP...
2017: Porcine Health Management
https://www.readbyqxmd.com/read/28484709/pharmacokinetic-and-pharmacodynamic-evaluation-of-marbofloxacin-in-pig-against-korean-local-isolates-of-actinobacillus-pleuropneumoniae
#17
Md Akil Hossain, Hae-Chul Park, Kyunghun Jeong, Yang Ho Jang, Dae Gyun Kim, JeongWoo Kang, Kwang-Jick Lee
The pharmacokinetics of marbofloxacin in pigs after intravenous (i.v.), intramuscular (i.m.), and peroral (p.o.) administration and pharmacokinetic/pharmacodynamic indices of this drug against Korean local isolates of Actinobacillus pleuropneumoniae were determined in this study. Marbofloxacin (2.50 mg/kg of body weight) was administered, and blood samples were collected with designated time intervals. Plasma-extracted marbofloxacin was injected into the LC-MS/MS system. The in vitro and ex vivo antibacterial activities of marbofloxacin were evaluated against 20 isolates of A...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28448619/pyridoxal-phosphate-synthases-pdxs-pdxt-are-required-for-actinobacillus-pleuropneumoniae-viability-stress-tolerance-and-virulence
#18
Fang Xie, Gang Li, Yalei Wang, Yanhe Zhang, Long Zhou, Chengcheng Wang, Shuanghong Liu, Siguo Liu, Chunlai Wang
Pyridoxal 5'-phosphate (PLP) is an essential cofactor for numerous enzymes involved in a diversity of cellular processes in living organisms. Previous analysis of the Actinobacillus pleuropneumoniae S-8 genome sequence revealed the presence of pdxS and pdxT genes, which are implicated in deoxyxylulose 5-phosphate (DXP)-independent pathway of PLP biosynthesis; however, little is known about their roles in A. pleuropneumoniae pathogenicity. Our data demonstrated that A. pleuropneumoniae could synthesize PLP by PdxS and PdxT enzymes...
2017: PloS One
https://www.readbyqxmd.com/read/28381551/production-of-homogeneous-glycoprotein-with-multisite-modifications-by-an-engineered-n-glycosyltransferase-mutant
#19
Qitao Song, Zhigang Wu, Yueyuan Fan, Woran Song, Peiru Zhang, Li Wang, Faxing Wang, Yangyang Xu, Peng G Wang, Jiansong Cheng
Naturally occurring N-glycoproteins exhibit glycoform heterogeneity with respect to N-glycan sequon occupancy (macroheterogeneity) and glycan structure (microheterogeneity). However, access to well-defined glycoproteins is always important for both basic research and therapeutic purposes. As a result, there has been a substantial effort to identify and understand the catalytic properties of N-glycosyltransferases, enzymes that install the first glycan on the protein chain. In this study we found that ApNGT, a newly discovered cytoplasmic N-glycosyltransferase from Actinobacillus pleuropneumoniae, has strict selectivity toward the residues around the Asn of N-glycosylation sequon by screening a small library of synthetic peptides...
May 26, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28348142/survey-of-susceptibility-to-marbofloxacin-in-bacteria-isolated-from-diseased-pigs-in-europe
#20
F El Garch, S Kroemer, D Galland, I Morrissey, F Woehrle
A monitoring programme of marbofloxacin susceptibility of bacteria from Europe causing respiratory tract infection and meningitis in pigs has been active since 1994 and 2002, respectively. Monitoring digestive, metritis and urinary tract infection (UTI) in pigs has been active since 2005 and susceptibility results until 2013 are presented. Minimum inhibitory concentration (MIC) was determined by broth microdilution. For MIC interpretation, Vétoquinol-evaluated breakpoints were applied. For digestive pathogens, Escherichia coli and Salmonella species (1717 and 300 isolates, respectively) exhibited 7...
June 17, 2017: Veterinary Record
keyword
keyword
89097
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"