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actinobacillus pleuropneumoniae

Ying Li, Sanjie Cao, Luhua Zhang, Gee W Lau, Yiping Wen, Rui Wu, Qin Zhao, Xiaobo Huang, Qigui Yan, Yong Huang, Xintian Wen
Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, a significant disease that causes serious economic losses to the swine industry worldwide. Persistent infections caused by bacterial biofilms are recalcitrant to treat because of the particular drug resistance of biofilm-dwelling cells. TolC, a key component of multidrug efflux pumps, are responsible for multidrug resistance (MDR) in many Gram-negative bacteria. In this study, we identified two TolC-like proteins, TolC1 and TolC2, in A...
2016: Frontiers in Microbiology
Fei Zhang, Sanjie Cao, Zhuang Zhu, Yusheng Yang, Xintian Wen, Yung-Fu Chang, Xiaobo Huang, Rui Wu, Yiping Wen, Qigui Yan, Yong Huang, Xiaoping Ma, Qin Zhao
Six in vivo-induced (IVI) antigens-RnhB, GalU, GalT, Apl_1061, Apl_1166, and HflX were selected for a vaccine trial in a mouse model. The results showed that the IgG levels in each immune group was significantly higher than that of the negative control (P < 0.001). Except rRnhB group, proliferation of splenocytes was observed in all immunized groups and a relatively higher proliferation activity was observed in rGalU and rGalT groups (P < 0.05). In the rGalT vaccinated group, the proportion of CD4+ T cells in spleen was significant higher than that of negative control (P < 0...
2016: Frontiers in Microbiology
Yannick D N Tremblay, Josée Labrie, Sonia Chénier, Mario Jacques
Actinobacillus pleuropneumoniae causes porcine pleuropneumonia and forms biofilms in vitro on abiotic surfaces; however, presence of biofilms during infections has not been documented. The aim of this study was to use a species-specific fluorescent oligonucleotide probe and confocal microscopy to localize A. pleuropneumoniae in the lungs of two naturally infected pigs. Actinobacillus pleuropneumoniae was detected by fluorescence in situ hybridization and observed to grow as aggregates (~30-45 μm) during a natural infection...
October 28, 2016: Microbial Biotechnology
Chiung-Wen Hsu, Siou-Cen Li, Nai-Yun Chang, Zeng-Weng Chen, Jiunn-Wang Liao, Ter-Hsin Chen, Jyh-Perng Wang, Jiunn-Horng Lin, Shih-Ling Hsuan
Actinobacillus pleuropneumoniae is a crucial respiratory pathogen that causes fibrinous, hemorrhagic, necrotizing pleuropneumonia in pigs. A. pleuropneumoniae exotoxins (ApxI to IV) are the major virulence factors contributing to A. pleuropneumoniae pathogenesis. Previously, we demonstrated that ApxI induces the expression of proinflammatory cytokines in porcine alveolar macrophages (PAMs) via the mitogen-activated protein kinases (MAPKs) p38 and cJun NH2-terminal kinase (JNK). Nonetheless, the role of nuclear factor (NF)-κB-a transcription factor widely implicated in immune and inflammatory responses-in ApxI-elicited cytokine production has yet to be defined...
November 15, 2016: Veterinary Microbiology
Per Wallgren, Erik Nörregård, Benedicta Molander, Maria Persson, Carl-Johan Ehlorsson
BACKGROUND: Respiratory illness is traditionally regarded as the disease of the growing pig, and has historically mainly been associated to bacterial infections with focus on Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae. These bacteria still are of great importance, but continuously increasing herd sizes have complicated the scenario and the influence of secondary invaders may have been increased. The aim of this study was to evaluate the presence of A. pleuropneumoniae and M...
October 4, 2016: Acta Veterinaria Scandinavica
Hiroya Ito, Torata Ogawa, Dai Fukamizu, Yuiko Morinaga, Masahiro Kusumoto
The aim of our study was to reveal the molecular basis of the serologic nontypeability of 2 Actinobacillus pleuropneumoniae field isolates. Nine field strains of A. pleuropneumoniae, the causative agent of porcine pleuropneumonia, were isolated from pigs raised on the same farm and sent to our diagnostic laboratory for serotyping. Seven of the 9 strains were identified as serovar 15 strains by immunodiffusion tests. However, 2 strains, designated FH24-2 and FH24-5, could not be serotyped with antiserum prepared against serovars 1-15...
November 2016: Journal of Veterinary Diagnostic Investigation
Ying Li, Sanjie Cao, Luhua Zhang, Jianlin Yuan, Gee W Lau, Yiping Wen, Rui Wu, Qin Zhao, Xiaobo Huang, Qigui Yan, Yong Huang, Xintian Wen
Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, a major cause of economic loss in swine industry worldwide. TolC, the key component of multidrug efflux pumps and type I secretion systems, has been well-studied as an exit duct for numerous substances in many Gram-negative bacteria. By contrast, little is known on the role of TolC in biofilm formation. In this study, a ΔtolC mutant was used to examine the importance of TolC in biofilm formation of A. pleuropneumoniae...
2016: PloS One
Ingrid D E van Dixhoorn, Inonge Reimert, Jenny Middelkoop, J Elizabeth Bolhuis, Henk J Wisselink, Peter W G Groot Koerkamp, Bas Kemp, Norbert Stockhofe-Zurwieden
Until today, anti-microbial drugs have been the therapy of choice to combat bacterial diseases. Resistance against antibiotics is of growing concern in man and animals. Stress, caused by demanding environmental conditions, can reduce immune protection in the host, influencing the onset and outcome of infectious diseases. Therefore psychoneuro-immunological intervention may prove to be a successful approach to diminish the impact of diseases and antibiotics use. This study was designed to investigate the effect of social and environmental enrichment on the impact of disease, referred to as "disease susceptibility", in pigs using a co-infection model of PRRSV and A...
2016: PloS One
Yeotaek Cheong, Changin Oh, Kunkyu Lee, Ki-Hyun Cho
Oral fluid analysis for monitoring herd is an interest in commercial pig farms in Korea. The aim of this study was to investigate the positive rates and correlation of eight pathogens associated with porcine respiratory disease complex (PRDC) by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. The samples collected by rope-chewing method were analyzed with reverse-transcription polymerase chain reaction (RT-PCR) or standard PCR depending on microorganisms. The pathogens were divided into virus group and bacteria group...
September 1, 2016: Journal of Veterinary Science
Y Li, J T Bossé, S M Williamson, D J Maskell, A W Tucker, B W Wren, A N Rycroft, P R Langford
No abstract text is available yet for this article.
September 17, 2016: Veterinary Record
C Fablet, C Marois-Créhan, B Grasland, G Simon, N Rose
Factors associated with porcine reproductive and respiratory syndrome virus (PRRSV) infection were investigated in 109 herds. Serums from four batches of pigs (4, 10, 16 and 22 weeks, 15 pigs/batch) were tested by ELISA for PRRSV antibodies. Infection by Mycoplasma hyopneumoniae (Mhp), Actinobacillus pleuropneumoniae, H1N1 and H1N2 swine influenza A viruses (swIAV) and PCV2 were detected by specific serological or PCR tests. Data related to herd characteristics, biosecurity, management housing and climatic conditions were collected during a herd visit...
August 30, 2016: Veterinary Microbiology
Vladimir Sarpe, Atefeh Rafiei, Morgan Hepburn, Nicholas Ostan, Anthony B Schryvers, David C Schriemer
The Mass Spec Studio package was designed to support the extraction of hydrogen-deuterium exchange and covalent labeling data for a range of mass spectrometry (MS)-based workflows, to integrate with restraint-driven protein modeling activities. In this report, we present an extension of the underlying Studio framework and provide a plug-in for crosslink (XL) detection. To accommodate flexibility in XL methods and applications, while maintaining efficient data processing, the plug-in employs a peptide library reduction strategy via a presearch of the tandem-MS data...
September 2016: Molecular & Cellular Proteomics: MCP
Ciro C Rossi, Janine T Bossé, Yanwen Li, Adam A Witney, Kate A Gould, Paul R Langford, Denise M S Bazzolli
Bacterial regulatory small RNAs (sRNAs) play important roles in gene regulation and are frequently connected to the expression of virulence factors in diverse bacteria. Only a few sRNAs have been described for Pasteurellaceae pathogens and no in-depth analysis of sRNAs has been described for Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, responsible for considerable losses in the swine industry. To search for sRNAs in A. pleuropneumoniae, we developed a strategy for the computational analysis of the bacterial genome by using four algorithms with different approaches, followed by experimental validation...
September 2016: RNA
Janine T Bossé, Yanwen Li, Roberto Fernandez Crespo, Roy R Chaudhuri, Jon Rogers, Matthew T G Holden, Duncan J Maskell, Alexander W Tucker, Brendan W Wren, Andrew N Rycroft, Paul R Langford
ICEApl1 was identified in the whole genome sequence of MIDG2331, a tetracycline-resistant (MIC = 8 mg/L) serovar 8 clinical isolate of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. PCR amplification of virB4, one of the core genes involved in conjugation, was used to identify other A. pleuropneumoniae isolates potentially carrying ICEApl1. MICs for tetracycline were determined for virB4 positive isolates, and shotgun whole genome sequence analysis was used to confirm presence of the complete ICEApl1...
2016: Frontiers in Microbiology
Dae Dayao, J S Gibson, P J Blackall, C Turni
OBJECTIVE: To identify genes associated with the observed antimicrobial resistance in Actinobacillus pleuropneumoniae, Haemophilus parasuis and Pasteurella multocida isolated from Australian pigs. DESIGN: Isolates with known phenotypic resistance to β-lactams, macrolides and tetracycline were screened for the presence of antimicrobial resistance genes. PROCEDURE: A total of 68 A. pleuropneumoniae, 62 H. parasuis and 20 P. multocida isolates exhibiting phenotypic antimicrobial resistance (A...
July 2016: Australian Veterinary Journal
Abraham Loera-Muro, Mario Jacques, Francisco J Avelar-González, Josée Labrie, Yannick D N Tremblay, Ricardo Oropeza-Navarro, Alma L Guerrero-Barrera
BACKGROUND: Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, which causes important worldwide economic losses in the swine industry. Several respiratory tract infections are associated with biofilm formation, and A. pleuropneumoniae has the ability to form biofilms in vitro. Biofilms are structured communities of bacterial cells enclosed in a self-produced polymer matrix that are attached to an abiotic or biotic surface. Virtually all bacteria can grow as a biofilm, and multi-species biofilms are the most common form of microbial growth in nature...
2016: BMC Microbiology
Long-Xian Lv, Dai-Qiong Fang, Ding Shi, De-Ying Chen, Ren Yan, Yi-Xin Zhu, Yan-Fei Chen, Li Shao, Fei-Fei Guo, Wen-Rui Wu, Ang Li, Hai-Yan Shi, Xia-Wei Jiang, Hui-Yong Jiang, Yong-Hong Xiao, Shu-Sen Zheng, Lan-Juan Li
We selected 42 early-stage primary biliary cirrhosis (PBC) patients and 30 healthy controls (HC). Metagenomic sequencing of the 16S rRNA gene was used to characterize the fecal microbiome. UPLC-MS/MS assaying of small molecules was used to characterize the metabolomes of the serum, urine and feces. Liquid chip assaying of serum cytokines was used to characterize the immune profiles. The gut of PBC patients were depleted of some potentially beneficial bacteria, such as Acidobacteria, Lachnobacterium sp., Bacteroides eggerthii and Ruminococcus bromii, but were enriched in some bacterial taxa containing opportunistic pathogens, such as γ-Proteobacteria, Enterobacteriaceae, Neisseriaceae, Spirochaetaceae, Veillonella, Streptococcus, Klebsiella, Actinobacillus pleuropneumoniae, Anaeroglobus geminatus, Enterobacter asburiae, Haemophilus parainfluenzae, Megasphaera micronuciformis and Paraprevotella clara...
July 2016: Environmental Microbiology
Skander Hathroubi, Francis Beaudry, Chantale Provost, Léa Martelet, Mariela Segura, Carl A Gagnon, Mario Jacques
Actinobacillus pleuropneumoniae (APP), the etiologic agent of porcine pleuropneumonia, forms biofilms on biotic and abiotic surfaces. APP biofilms confers resistance to antibiotics. To our knowledge, no studies have examined the role of APP biofilm in immune evasion and infection persistence. This study was undertaken to (i) investigate biofilm-associated LPS modifications occurring during the switch to biofilm mode of growth; and (ii) characterize pro-inflammatory cytokines expression in porcine pulmonary alveolar macrophages (PAMs) and proliferation in porcine PBMCs challenged with planktonic or biofilm APP cells...
July 2016: Innate Immunity
Mi-Young Kim, Tae-Geum Kim, Moon-Sik Yang
Actinobacillus pleuropneumoniae is a major etiological agent that is responsible for swine pleuropneumonia, a highly contagious respiratory infection that causes severe economic losses in the swine production industry. ApxIIA is one of the virulence factors in A. pleuropneumoniae and has been considered as a candidate for developing a vaccine against the bacterial infection. A gene encoding an ApxIIA fragment (amino acids 439-801) was modified based on a plant-optimized codon and constructed into a plant expression vector under the control of a promoter and the 3' UTR of the rice amylase 3D gene...
May 20, 2016: Protein Expression and Purification
Ho To, Shinya Nagai, Akira Iwata, Tomohiro Koyama, Atsushi Oshima, Nobuyuki Tsutsumi
Apx toxins produced by Actinobacillus pleuropneumoniae are essential components of new generation vaccines. In this study, apxIIA and apxIIIA genes of serovars 2, 3, 4, 6, 8 and 15 were cloned and sequenced. Amino acid sequences of ApxIIA proteins of serovars 2, 3, 4, 6, 8 and 15 were almost identical to those of serovars 1, 5, 7, 9 and 11-13. Immunoblot analysis showed that rApxIIA from serovars 2 and 15 reacts strongly with sera from animals infected with various serovars. Sequence analysis revealed that ApxIIIA proteins has two variants, one in strains of serovar 2 and the other in strains of serovars 3, 4, 6, 8 and 15...
July 2016: Microbiology and Immunology
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