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actinobacillus pleuropneumoniae

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https://www.readbyqxmd.com/read/28101885/what-is-the-true-in%C3%A2-vitro-potency-of-oxytetracycline-for-the-pig-pneumonia-pathogens-actinobacillus-pleuropneumoniae-and-pasteurella-multocida
#1
L Dorey, S Hobson, P Lees
The pharmacodynamics of oxytetracycline was determined for pig respiratory tract pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida. Indices of potency were determined for the following: (i) two matrices, broth and pig serum; (ii) five overlapping sets of twofold dilutions; and (iii) a high strength starting culture. For A. pleuropneumoniae, minimum inhibitory concentration (MIC) was similar for the two matrices, but for P. multocida, differences were marked and significantly different. MIC and minimum bactericidal concentration (MBC) serum: broth ratios for A...
January 18, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28090673/pharmacokinetic-pharmacodynamic-integration-and-modelling-of-oxytetracycline-for-the-porcine-pneumonia-pathogens-actinobacillus-pleuropneumoniae-and-pasteurella-multocida
#2
L Dorey, L Pelligand, Z Cheng, P Lees
Pharmacokinetic-pharmacodynamic (PK/PD) integration and modelling were used to predict dosage schedules of oxytetracycline for two pig pneumonia pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida. Minimum inhibitory concentration (MIC) and mutant prevention concentration (MPC) were determined in broth and porcine serum. PK/PD integration established ratios of average concentration over 48 h (Cav0-48 h )/MIC of 5.87 and 0.27 μg/mL (P. multocida) and 0.70 and 0.85 μg/mL (A. pleuropneumoniae) for broth and serum MICs, respectively...
January 16, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28077594/the-n-linking-glycosylation-system-from-actinobacillus-pleuropneumoniae-is-required-for-adhesion-and-has-potential-use-in-glycoengineering
#3
Jon Cuccui, Vanessa S Terra, Janine T Bossé, Andreas Naegeli, Sherif Abouelhadid, Yanwen Li, Chia-Wei Lin, Prerna Vohra, Alexander W Tucker, Andrew N Rycroft, Duncan J Maskell, Markus Aebi, Paul R Langford, Brendan W Wren
Actinobacillus pleuropneumoniae is a mucosal respiratory pathogen causing contagious porcine pleuropneumonia. Pathogenesis studies have demonstrated a major role for the capsule, exotoxins and outer membrane proteins. Actinobacillus pleuropneumoniae can also glycosylate proteins, using a cytoplasmic N-linked glycosylating enzyme designated NGT, but its transcriptional arrangement and role in virulence remains unknown. We investigated the NGT locus and demonstrated that the putative transcriptional unit consists of rimO, ngt and a glycosyltransferase termed agt...
January 2017: Open Biology
https://www.readbyqxmd.com/read/28061786/attenuated-actinobacillus-pleuropneumoniae-double-deletion-mutant-s-8%C3%A2-clpp-apxiic-confers-protection-against-homologous-or-heterologous-strain-challenge
#4
Fang Xie, Gang Li, Long Zhou, Yanhe Zhang, Ning Cui, Siguo Liu, Chunlai Wang
BACKGROUND: Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, which leads to large economic losses to the swine industry worldwide. In this study, S-8△clpP△apxIIC, a double-deletion mutant of A. pleuropneumoniae was constructed, and its safety and protective efficacy were evaluated in pigs. RESULTS: The S-8△clpP△apxIIC mutant exhibited attenuated virulence in a murine (BALB/c) model, and caused no detrimental effects on pigs even at a dose of up to 1...
January 6, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28053219/a-unique-capsule-locus-in-the-newly-designated-actinobacillus-pleuropneumoniae-serovar-16-and-development-of-a-diagnostic-pcr
#5
Janine T Bossé, Yanwen Li, Rita Sárközi, Marcelo Gottschalk, Øystein Angen, Katerina Nedbalcova, Andrew N Rycroft, László Fodor, Paul R Langford
Actinobacillus pleuropneumoniae causes pleuropneumonia, an economically significant, lung disease of pigs. Recently, isolates of A. pleuropneumoniae that were serologically distinct from the previously characterized 15 serovars were described, and a proposal put forward that they comprised a new serovar 16. Here we used whole genome sequencing of the proposed serovar 16 reference strain A-85/14 to confirm presence of a unique capsular polysaccharide biosynthetic locus. For molecular diagnostics, primers were designed from the capsule locus of A-85/14 and a PCR formulated that differentiated serovar 16 isolates from all 15 known serovars, and other common respiratory pathogenic/commensal bacteria of pigs...
January 4, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27940285/potency-of-marbofloxacin-for-pig-pneumonia-pathogens-actinobacillus-pleuropneumoniae-and-pasteurella-multocida-comparison-of-growth-media
#6
L Dorey, S Hobson, P Lees
Pharmacodynamic properties of marbofloxacin were established for six isolates each of the pig respiratory tract pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida. Three in vitro indices of potency were determined; Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Mutant Prevention Concentration (MPC). For MIC determination Clinical Laboratory Standards Institute guidelines were modified in three respects: (1) comparison was made between two growth media, an artificial broth and pig serum; (2) a high inoculum count was used to simulate heavy clinical bacteriological loads; and (3) five overlapping sets of two-fold dilutions were used to improve accuracy of determinations...
November 25, 2016: Research in Veterinary Science
https://www.readbyqxmd.com/read/27940254/apa2h1-the-first-head-domain-of-apa2-trimeric-autotransporter-adhesin-activates-mouse-bone-marrow-derived-dendritic-cells-and-immunization-with-apa2h1-protects-against-actinobacillus-pleuropneumoniae-infection
#7
Wanhai Qin, Lei Wang, Ruidong Zhai, Qiuyue Ma, Jianfang Liu, Chuntong Bao, Diangang Sun, Hu Zhang, Changjiang Sun, Xin Feng, Jingmin Gu, Chongtao Du, Wenyu Han, P R Langford, Liancheng Lei
Actinobacillus pleuropneumoniae is the causative pathogen of porcine pleuropneumonia, which results in large economic losses in the pig industry worldwide. There are, however, no effective subunit vaccines are available in the market owing to the various serotypes and the absence of cross-protection against this pathogen. Therefore, the selection of protective components is of great significance for vaccine development. We previously showed that trimeric autotransporter adhesins are important virulence factors of A...
January 2017: Molecular Immunology
https://www.readbyqxmd.com/read/27938711/activity-of-florfenicol-for-actinobacillus-pleuropneumoniae-and-pasteurella-multocida-using-standardised-versus-non-standardised-methodology
#8
L Dorey, S Hobson, P Lees
Four indices of antimicrobial potency were determined for florfenicol and the pig pneumonia pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), mutant prevention concentration (MPC) and time-kill curves were determined in two matrices, broth and pig serum. Five overlapping sets of two-fold dilutions were used to increase accuracy of the measurements. MIC and MBC serum:broth ratios for A. pleuropneumoniae were 0...
December 2016: Veterinary Journal
https://www.readbyqxmd.com/read/27822201/a-tolc-like-protein-of-actinobacillus-pleuropneumoniae-is-involved-in-antibiotic-resistance-and-biofilm-formation
#9
Ying Li, Sanjie Cao, Luhua Zhang, Gee W Lau, Yiping Wen, Rui Wu, Qin Zhao, Xiaobo Huang, Qigui Yan, Yong Huang, Xintian Wen
Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, a significant disease that causes serious economic losses to the swine industry worldwide. Persistent infections caused by bacterial biofilms are recalcitrant to treat because of the particular drug resistance of biofilm-dwelling cells. TolC, a key component of multidrug efflux pumps, are responsible for multidrug resistance (MDR) in many Gram-negative bacteria. In this study, we identified two TolC-like proteins, TolC1 and TolC2, in A...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27818646/immunoprotective-efficacy-of-six-in-vivo-induced-antigens-against-actinobacillus-pleuropneumoniae-as-potential-vaccine-candidates-in-murine-model
#10
Fei Zhang, Sanjie Cao, Zhuang Zhu, Yusheng Yang, Xintian Wen, Yung-Fu Chang, Xiaobo Huang, Rui Wu, Yiping Wen, Qigui Yan, Yong Huang, Xiaoping Ma, Qin Zhao
Six in vivo-induced (IVI) antigens-RnhB, GalU, GalT, Apl_1061, Apl_1166, and HflX were selected for a vaccine trial in a mouse model. The results showed that the IgG levels in each immune group was significantly higher than that of the negative control (P < 0.001). Except rRnhB group, proliferation of splenocytes was observed in all immunized groups and a relatively higher proliferation activity was observed in rGalU and rGalT groups (P < 0.05). In the rGalT vaccinated group, the proportion of CD4+ T cells in spleen was significant higher than that of negative control (P < 0...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27790837/actinobacillus-pleuropneumoniae-grows-as-aggregates-in-the-lung-of-pigs-is-it-time-to-refine-our-in%C3%A2-vitro-biofilm-assays
#11
Yannick D N Tremblay, Josée Labrie, Sonia Chénier, Mario Jacques
Actinobacillus pleuropneumoniae causes porcine pleuropneumonia and forms biofilms in vitro on abiotic surfaces; however, presence of biofilms during infections has not been documented. The aim of this study was to use a species-specific fluorescent oligonucleotide probe and confocal microscopy to localize A. pleuropneumoniae in the lungs of two naturally infected pigs. Actinobacillus pleuropneumoniae was detected by fluorescence in situ hybridization and observed to grow as aggregates (~30-45 μm) during a natural infection...
October 28, 2016: Microbial Biotechnology
https://www.readbyqxmd.com/read/27771058/involvement-of-nf-%C3%AE%C2%BAb-in-regulation-of-actinobacillus-pleuropneumoniae-exotoxin-apxi-induced-proinflammatory-cytokine-production-in-porcine-alveolar-macrophages
#12
Chiung-Wen Hsu, Siou-Cen Li, Nai-Yun Chang, Zeng-Weng Chen, Jiunn-Wang Liao, Ter-Hsin Chen, Jyh-Perng Wang, Jiunn-Horng Lin, Shih-Ling Hsuan
Actinobacillus pleuropneumoniae is a crucial respiratory pathogen that causes fibrinous, hemorrhagic, necrotizing pleuropneumonia in pigs. A. pleuropneumoniae exotoxins (ApxI to IV) are the major virulence factors contributing to A. pleuropneumoniae pathogenesis. Previously, we demonstrated that ApxI induces the expression of proinflammatory cytokines in porcine alveolar macrophages (PAMs) via the mitogen-activated protein kinases (MAPKs) p38 and cJun NH2-terminal kinase (JNK). Nonetheless, the role of nuclear factor (NF)-κB-a transcription factor widely implicated in immune and inflammatory responses-in ApxI-elicited cytokine production has yet to be defined...
November 15, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27716292/serological-patterns-of-actinobacillus-pleuropneumoniae-mycoplasma-hyopneumoniae-pasteurella-multocida-and-streptococcus-suis-in-pig-herds-affected-by-pleuritis
#13
Per Wallgren, Erik Nörregård, Benedicta Molander, Maria Persson, Carl-Johan Ehlorsson
BACKGROUND: Respiratory illness is traditionally regarded as the disease of the growing pig, and has historically mainly been associated to bacterial infections with focus on Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae. These bacteria still are of great importance, but continuously increasing herd sizes have complicated the scenario and the influence of secondary invaders may have been increased. The aim of this study was to evaluate the presence of A. pleuropneumoniae and M...
October 4, 2016: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/27694188/nucleotide-sequence-analysis-of-a-dna-region-involved-in-capsular-polysaccharide-biosynthesis-reveals-the-molecular-basis-of-the-nontypeability-of-two-actinobacillus-pleuropneumoniae-isolates
#14
Hiroya Ito, Torata Ogawa, Dai Fukamizu, Yuiko Morinaga, Masahiro Kusumoto
The aim of our study was to reveal the molecular basis of the serologic nontypeability of 2 Actinobacillus pleuropneumoniae field isolates. Nine field strains of A. pleuropneumoniae, the causative agent of porcine pleuropneumonia, were isolated from pigs raised on the same farm and sent to our diagnostic laboratory for serotyping. Seven of the 9 strains were identified as serovar 15 strains by immunodiffusion tests. However, 2 strains, designated FH24-2 and FH24-5, could not be serotyped with antiserum prepared against serovars 1-15...
November 2016: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/27681876/absence-of-tolc-impairs-biofilm-formation-in-actinobacillus-pleuropneumoniae-by-reducing-initial-attachment
#15
Ying Li, Sanjie Cao, Luhua Zhang, Jianlin Yuan, Gee W Lau, Yiping Wen, Rui Wu, Qin Zhao, Xiaobo Huang, Qigui Yan, Yong Huang, Xintian Wen
Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, a major cause of economic loss in swine industry worldwide. TolC, the key component of multidrug efflux pumps and type I secretion systems, has been well-studied as an exit duct for numerous substances in many Gram-negative bacteria. By contrast, little is known on the role of TolC in biofilm formation. In this study, a ΔtolC mutant was used to examine the importance of TolC in biofilm formation of A. pleuropneumoniae...
2016: PloS One
https://www.readbyqxmd.com/read/27606818/enriched-housing-reduces-disease-susceptibility-to-co-infection-with-porcine-reproductive-and-respiratory-virus-prrsv-and-actinobacillus-pleuropneumoniae-a-pleuropneumoniae-in-young-pigs
#16
Ingrid D E van Dixhoorn, Inonge Reimert, Jenny Middelkoop, J Elizabeth Bolhuis, Henk J Wisselink, Peter W G Groot Koerkamp, Bas Kemp, Norbert Stockhofe-Zurwieden
Until today, anti-microbial drugs have been the therapy of choice to combat bacterial diseases. Resistance against antibiotics is of growing concern in man and animals. Stress, caused by demanding environmental conditions, can reduce immune protection in the host, influencing the onset and outcome of infectious diseases. Therefore psychoneuro-immunological intervention may prove to be a successful approach to diminish the impact of diseases and antibiotics use. This study was designed to investigate the effect of social and environmental enrichment on the impact of disease, referred to as "disease susceptibility", in pigs using a co-infection model of PRRSV and A...
2016: PloS One
https://www.readbyqxmd.com/read/27586468/a-survey-of-porcine-respiratory-disease-complex-prdc-associated-pathogens-among-commercial-pig-farms-of-korea-via-oral-fluid-method
#17
Yeotaek Cheong, Changin Oh, Kunkyu Lee, Ki-Hyun Cho
Oral fluid analysis for monitoring herd is an interest in commercial pig farms in Korea. The aim of this study was to investigate the positive rates and correlation of eight pathogens associated with porcine respiratory disease complex (PRDC) by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. The samples collected by rope-chewing method were analyzed with reverse-transcription polymerase chain reaction (RT-PCR) or standard PCR depending on microorganisms. The pathogens were divided into virus group and bacteria group...
September 1, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/27531715/actinobacillus-pleuropneumoniae-serovar-8-predominates-in-england-and-wales
#18
Y Li, J T Bossé, S M Williamson, D J Maskell, A W Tucker, B W Wren, A N Rycroft, P R Langford
No abstract text is available yet for this article.
September 17, 2016: Veterinary Record
https://www.readbyqxmd.com/read/27527759/factors-associated-with-herd-level-prrsv-infection-and-age-time-to-seroconversion-in-farrow-to-finish-herds
#19
C Fablet, C Marois-Créhan, B Grasland, G Simon, N Rose
Factors associated with porcine reproductive and respiratory syndrome virus (PRRSV) infection were investigated in 109 herds. Serums from four batches of pigs (4, 10, 16 and 22 weeks, 15 pigs/batch) were tested by ELISA for PRRSV antibodies. Infection by Mycoplasma hyopneumoniae (Mhp), Actinobacillus pleuropneumoniae, H1N1 and H1N2 swine influenza A viruses (swIAV) and PCV2 were detected by specific serological or PCR tests. Data related to herd characteristics, biosecurity, management housing and climatic conditions were collected during a herd visit...
August 30, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27412762/high-sensitivity-crosslink-detection-coupled-with-integrative-structure-modeling-in-the-mass-spec-studio
#20
Vladimir Sarpe, Atefeh Rafiei, Morgan Hepburn, Nicholas Ostan, Anthony B Schryvers, David C Schriemer
The Mass Spec Studio package was designed to support the extraction of hydrogen-deuterium exchange and covalent labeling data for a range of mass spectrometry (MS)-based workflows, to integrate with restraint-driven protein modeling activities. In this report, we present an extension of the underlying Studio framework and provide a plug-in for crosslink (XL) detection. To accommodate flexibility in XL methods and applications, while maintaining efficient data processing, the plug-in employs a peptide library reduction strategy via a presearch of the tandem-MS data...
September 2016: Molecular & Cellular Proteomics: MCP
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