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https://www.readbyqxmd.com/read/29906781/structural-optimization-and-amorphous-calcium-phosphate-mineralization-in-sensory-setae-of-a-terrestrial-crustacean-isopoda-oniscidea
#1
Miloš Vittori, Vesna Srot, Birgit Bussmann, Felicitas Predel, Peter A van Aken, Jasna Štrus
Terrestrial isopods possess large sensory setae on their walking legs. Increased fracture resistance of these elongated structures is of crucial importance, making the exoskeleton forming the setae an interesting durable material that may inspire biomimetic designs. We studied the cuticle of the sensory setae with analytical electron microscopy in order to gain detailed insights into its structure and composition at the nanometer scale and identify features that increase the fracture resistance of these minute skeletal elements...
June 9, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29906780/in-vivo-evaluation-of-the-response-of-galleria-mellonella-larvae-to-novel-copper-ii-phenanthroline-phenazine-complexes
#2
Garret Rochford, Zara Molphy, Niall Browne, Carla Surlis, Michael Devereux, Malachy McCann, Andrew Kellett, Orla Howe, Kevin Kavanagh
Herein we report the in-vivo characterisation and metabolic changes in Galleria mellonella larvae to a series of bis-chelate copper(II) phenanthroline-phenazine cationic complexes of [Cu(phen)2 ]2+ (Cu-Phen), [Cu(DPQ)(Phen)]2+ (Cu-DPQ-Phen) and [Cu(DPPZ)(Phen)]2+ (Cu-DPPZ-Phen) (where phen = 1,10-phenanthroline, DPQ = dipyrido[3,2-ƒ:2',3'-h]quinoxaline and DPPZ = dipyrido[3,2-a:2',3'-c]phenazine). Our aim was to investigate the influence of the systematic extension of the ligated phenazine ligand in the G...
June 1, 2018: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29904669/preliminary-in-vivo-magnetofection-data-using-magnetic-calcium-phosphate-nanoparticles-immobilizing-dna-and-iron-oxide-nanocrystals
#3
Quazi T H Shubhra, Ayako Oyane, Maki Nakamura, Sandra Puentes, Aiki Marushima, Hideo Tsurushima
The data reported herein are in association with our research article entitled "Rapid one-pot fabrication of magnetic calcium phosphate nanoparticles immobilizing DNA and iron oxide nanocrystals using injection solutions for magnetofection and magnetic targeting" (Shubhra et al. 2017) [1]. This article reports morphological and gene delivery ( in vitro and preliminary in vivo ) data of those calcium phosphate (CaP) naonparticles (NPs) with various iron oxide (IO) contents, named as CaP-Fe(1), CaP-Fe(2), CaP-Fe(3), CaP-Fe(4), and CaP-Fe(5), which were prepared via coprecipitation in supersaturated CaP solutions with nominal Fe concentrations 6...
June 2018: Data in Brief
https://www.readbyqxmd.com/read/29904023/modified-polymeric-nanoparticles-exert-in-vitro-antimicrobial-activity-against-oral-bacteria
#4
Manuel Toledano-Osorio, Jegdish P Babu, Raquel Osorio, Antonio L Medina-Castillo, Franklin García-Godoy, Manuel Toledano
Polymeric nanoparticles were modified to exert antimicrobial activity against oral bacteria. Nanoparticles were loaded with calcium, zinc and doxycycline. Ions and doxycycline release were measured by inductively coupled plasma optical emission spectrometer and high performance liquid chromatography. Porphyromonas gingivalis , Lactobacillus lactis , Streptoccocus mutans , gordonii and sobrinus were grown and the number of bacteria was determined by optical density. Nanoparticles were suspended in phosphate-buffered saline (PBS) at 10, 1 and 0...
June 14, 2018: Materials
https://www.readbyqxmd.com/read/29903718/calcitriol-accelerates-vascular-calcification-irrespective-of-vitamin-k-status-in-a-rat-model-of-ckd-with-hyperphosphatemia-and-secondary-hyperparathyroidism
#5
Kristin M McCabe, Jason G Zelt, Martin Kaufmann, Kimberly Laverty, Emilie Ward, Henry Barron, Glenville Jones, Michael A Adams, Rachel M Holden
Patients with chronic kidney disease have a markedly increased risk for developing cardiovascular disease. Non-traditional risk factors, such as increased phosphate retention, and deficiencies in vitamins D and K metabolism, likely play key roles in the development of vascular calcification during CKD progression. Calcitriol (1,25-(OH)2-D3) is a key transcriptional regulator of Matrix Gla protein (MGP), a vitamin K dependent protein that inhibits vascular calcification. The objective of this study was to determine if calcitriol treatment could inhibit the development of vascular calcification and if this inhibition was dependent on vitamin K status in a rat model of CKD...
June 14, 2018: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/29900589/mineral-and-bone-disorders-in-conventional-hemodialysis-challenges-and-solutions
#6
Takayuki Hamano
Despite the advent of cinacalcet and noncalcium-containing phosphate binders, controlling the progression of vascular calcification (VC) is still challenging. Recent reports demonstrate that carbamylation driven by high urea concentration aggravates VC, suggesting the importance of adequate dialysis in retarding its progression. Theoretically, other promising measures include the use of iron-based phosphate binders, vitamin K, and magnesium supplements, which should be investigated in future randomized controlled trials (RCTs), ideally with hard outcomes...
June 13, 2018: Seminars in Dialysis
https://www.readbyqxmd.com/read/29900374/data-on-pko-biodiesel-production-using-cao-catalyst-from-turkey-bones
#7
A A Ayoola, O S I Fayomi, I F Usoro
In this research paper the production of biodiesel from palm kernel oil (PKO) using CaO obtained from waste turkey bones (WTB) and analytical grade calcium oxide was investigated. Treated WTB was reduced to fine particulate size of <150 µm and then calcinated at 800 °C for 3 h to increase its catalytic activity by its conversion from Calcium phosphate hydroxide (Ca10 P6 O26 H2 ) to CaO. X-ray diffraction (XRD) and X-ray fluorescent (XRF) analysis of the analytical grade CaO, uncalcined and calcined WTB were carried out to establish their elemental chemical composition...
August 2018: Data in Brief
https://www.readbyqxmd.com/read/29900355/data-for-factor-analysis-of-hydro-geochemical-characteristics-of-groundwater-resources-in-iranshahr
#8
Hamed Biglari, Mehdi Saeidi, Kamaleddin Karimyan, Mohammad Reza Narooie, Hooshmand Sharafi
Detection of Hydrogeological and Hydro-geochemical changes affecting the quality of aquifer water is very important. The aim of this study was to determine the factor analysis of the hydro-geochemical characteristics of Iranshahr underground water resources during the warm and cool seasons. In this study, 248 samples (two-time repetitions) of ground water resources were provided at first by cluster-random sampling method during 2017 in the villages of Iranshahr city. After transferring the samples to the laboratory, concentrations of 13 important chemical parameters in those samples were determined according to o water and wastewater standard methods...
August 2018: Data in Brief
https://www.readbyqxmd.com/read/29899992/successful-use-of-cinacalcet-to-treat-parathyroid-related-hypercalcemia-in-two-pediatric-patients
#9
E Mogas, A Campos-Martorell, M Clemente, L Castaño, A Moreno-Galdó, D Yeste, A Carrascosa
Two pediatric patients with different causes of hyperparathyroidism are reported. First patient is a 13-year-old male with severe hypercalcemia due to left upper parathyroid gland adenoma. After successful surgery, calcium and phosphate levels normalized, but parathormone levels remained elevated. Further studies revealed a second adenoma in the right gland. The second patient is a 13-year-old female with uncommon hypercalcemia symptoms. Presence of pathogenic calcium-sensing receptor gene ( CASR ) mutation was found, resulting in diagnosis of symptomatic familial hypocalciuric hypercalcemia...
2018: Endocrinology, Diabetes & Metabolism Case Reports
https://www.readbyqxmd.com/read/29899969/bone-substitutes-a-review-of-their-characteristics-clinical-use-and-perspectives-for-large-bone-defects-management
#10
REVIEW
Gabriel Fernandez de Grado, Laetitia Keller, Ysia Idoux-Gillet, Quentin Wagner, Anne-Marie Musset, Nadia Benkirane-Jessel, Fabien Bornert, Damien Offner
Bone replacement might have been practiced for centuries with various materials of natural origin, but had rarely met success until the late 19th century. Nowadays, many different bone substitutes can be used. They can be either derived from biological products such as demineralized bone matrix, platelet-rich plasma, hydroxyapatite, adjunction of growth factors (like bone morphogenetic protein) or synthetic such as calcium sulfate, tri-calcium phosphate ceramics, bioactive glasses, or polymer-based substitutes...
January 2018: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/29896499/evaluation-of-water-quality-and-stability-in-the-drinking-water-distribution-network-in-the-azogues-city-ecuador
#11
Fernando García-Ávila, Lía Ramos-Fernández, Damián Pauta, Diego Quezada
This document presents the physical-chemical parameters with the objective of evaluating and analyzing the drinking water quality in the Azogues city applying the water quality index (WQI) and to research the water stability in the distribution network using corrosion indexes. Thirty samples were collected monthly for six months throughout the drinking water distribution network; turbidity, temperature, electric conductivity, pH, total dissolved solids, total hardness, calcium, magnesium, alkalinity, chlorides, nitrates, sulfates and phosphates were determined; the physical-chemical parameters were measured using standard methods...
June 2018: Data in Brief
https://www.readbyqxmd.com/read/29895376/effect-of-various-calcium-salts-on-non-heme-iron-bioavailability-in-fasted-women-of-childbearing-age
#12
Valeria Candia, Israel Ríos-Castillo, Frank Carrera-Gil, Berta Vizcarra, Manuel Olivares, Sotiris Chaniotakis, Fernando Pizarro
INTRODUCTION: Micronutrient deficiencies are one of the most important public health issues worldwide and iron (Fe) deficiency anemia is the most prevalent micronutrient deficiency. Iron deficiency often coexists with calcium deficiency and iron and calcium supplementation often overlap. This has led to investigations into the interaction between these two minerals, and whether calcium may inhibit iron absorption in the gut. OBJECTIVE: To determine the effect of various calcium salts on non-heme iron bioavailability in fasted women of childbearing age...
September 2018: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/29895327/conditioned-medium-from-bone-marrow-derived-mesenchymal-stem-cells-inhibits-vascular-calcification-through-blockade-of-the-bmp2-smad1-5-8-signaling-pathway
#13
Shuangshuang Wang, Siwang Hu, Jian Wang, Yahui Liu, Ruochi Zhao, Maoqing Tong, Hanbin Cui, Nan Wu, Xiaomin Chen
BACKGROUND: Arterial calcification is associated with cardiovascular disease as a complication of advanced atherosclerosis and is a significant contributor to cardiovascular morbidity and mortality. Osteoblastic differentiation of vascular smooth muscle cells (VSMCs) plays an important role in arterial calcification and is characterized by cellular necrosis, inflammation, and lipoprotein and phospholipid complexes, especially in atherosclerotic calcification. The conditioned medium from bone marrow-derived mesenchymal stem cells (MSC-CM) is well known as a rich source of autologous cytokines and is universally used for tissue regeneration in current clinical medicine...
June 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29894764/evaluation-of-dentin-desensitization-protocols-on-the-dentinal-surface-and-their-effects-on-the-dentin-bond-interface
#14
Wilfredo Gustavo Escalante-Otárola, Gabriela Mariana Castro-Núñez, Keren Cristina Fagundes Jordão-Basso, Bruno Martini Guimarães, Regina Guenka Palma-Dibb, Milton Carlos Kuga
OBJECTIVES: To evaluate the effect of desensitizing agent containing calcium phosphate nanoparticles on the bond strength ofetch-and-rinse adhesive system (Scotchbond Multi-Purpose), presence of precipitate, dentinal tubule obliteration and hybrid layer formation in dentin in comparison with potassium nitrate plus sodium fluoride or strontium chloride compounds. METHODS: 150 bovine incisors were treated with (n = 10): G1, Desensibilize Nano P (Ca3 (PO4 )2 +5%KNO3 +0...
June 9, 2018: Journal of Dentistry
https://www.readbyqxmd.com/read/29893081/-construction-of-her2-specific-car-t-cells-and-in-vitro-analysis-of-their-activity-to-suppress-tumor-cell-growth
#15
Yongqiang Li, Songyuan Yao, Yansheng Li, Mingkai Xu, Huiwen Zhang, Chenggang Zhang
CAR-T cell therapy that targets surface antigens to kill tumor cells specifically has recently become another cornerstone in tumor immunotherapy. In this study, a lentiviral expression plasmid of CAR targeting human epidermal growth factor receptor 2 (HER2) was constructed by genetic engineering. The recombinant plasmid was co-transfected with other packaging plasmids into HEK293T cells by calcium phosphate precipitation to generate lenti-car, which are CAR lentiviral particles. HER2-specific CAR-T cells were obtained by transducing human peripheral blood mononuclear cells with lenti-car...
May 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29892913/nanogels-of-carboxymethyl-chitosan-and-lysozyme-encapsulated-amorphous-calcium-phosphate-to-occlude-dentinal-tubules
#16
Jinhua Song, Haorong Wang, Yunqi Yang, Zuohui Xiao, Haibao Lin, Lichun Jin, Yan Xue, Mingli Lin, Fuyu Chen, Mengqi Zhu, Yanhong Zhao, Zhongjun Qiu, Yanqiu Li, Xu Zhang
This study aimed to develop of a rapid and effective method to occlude dentinal tubules using carboxymethyl chitosan and lysozyme (CMC/LYZ) nanogels with encapsulated amorphous calcium phosphate (ACP) based on the transformation of ACP to HAP. In this work, CMC/LYZ was used to stabilize ACP and form CMC/LYZ-ACP nanogels, and then the nanogel-encapsulated ACP was applied to exposed dentinal tubule surfaces. The morphology of the nanogels was examined by transmission electron microscopy (TEM). Distribution and quantity of elements in CMC/LYZ-ACP nanogels were determined by element mapping and energy dispersive X-Ray spectroscopy (EDX)...
June 11, 2018: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/29892909/sol-gel-based-calcium-phosphates-coatings-deposited-on-binary-ti-mo-alloys-modified-by-laser-beam-irradiation-for-biomaterial-clinical-applications
#17
Marcio Luiz Dos Santos, Carla Dos Santos Riccardi, Edson de Almeida Filho, Antonio C Guastaldi
Ti-15Mo alloy samples were irradiated by pulsed Yb: YAG laser beam under air and atmospheric pressure. Calcium phosphate coatings were deposited on the irradiated surfaces by the sol-gel method. The sol was prepared from the precursors Ca (NO3 )2 .4H2 O and H3 PO4 . The modified surfaces were submitted to heat treatment conditions at 350 and 600 °C. The results showed that the two conditions established have a sufficient energy to promote ablation on the laser beam irradiated surfaces. Likewise, it has been demonstrated the processes of fusion and fast solidification from the laser beam irradiation, under ambient atmosphere, inducing the formation of stoichiometric TiO2 and non-stoichiometric titanium oxides, including Ti3 O5 , TiO, Ti3 O and Ti6 O with different oxide percentages depending on the fluency used...
June 11, 2018: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/29892292/disease-associated-particulates-and-joint-inflammation-mechanistic-insights-and-potential-therapeutic-targets
#18
REVIEW
Olwyn R Mahon, Aisling Dunne
It is now well established that intra-articular deposition of endogenous particulates, such as osteoarthritis-associated basic calcium phosphate crystals, gout-associated monosodium urate crystals, and calcium deposition disease-associated calcium pyrophosphate crystals, contributes to joint destruction through the production of cartilage-degrading enzymes and pro-inflammatory cytokines. Furthermore, exogenous wear-debris particles, generated from prosthetic implants, drive periprosthetic osteolysis which impacts on the longevity of total joint replacements...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29892265/physiological-actions-of-fibroblast-growth-factor-23
#19
REVIEW
Reinhold G Erben
Fibroblast growth factor-23 (FGF23) is a bone-derived hormone suppressing phosphate reabsorption and vitamin D hormone synthesis in the kidney. At physiological concentrations of the hormone, the endocrine actions of FGF23 in the kidney are αKlotho-dependent, because high-affinity binding of FGF23 to FGF receptors requires the presence of the co-receptor αKlotho on target cells. It is well established that excessive concentrations of intact FGF23 in the blood lead to phosphate wasting in patients with normal kidney function...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29891861/in-vitro-and-in-vivo-evaluation-of-electrophoresis-aided-casein-phosphopeptide-amorphous-calcium-phosphate-remineralisation-system-on-ph-cycling-and-acid-etching-demineralised-enamel
#20
Yu Yuan Zhang, Hai Ming Wong, Colman P J McGrath, Quan Li Li
Casein phosphate-amorphous calcium phosphate (CPP-ACP), as a remineralisation agent, is extensively used in managing demineralised enamel; however, its remineralisation kinetics is low. This study aimed to improve remineralisation kinetics of CPP-ACP by introducing a rapid remineralisation method with electrophoresis. In vitro, a pH-cycling enamel model was used to test remineralisation potentials of electrophoresis-aided CPP-ACP. For verifying remineralisation potentials of electrophoresis-aided CPP-ACP in vivo in a rabbit model, acid-etched enamel surface on rabbit maxillary incisors was remineralised by electrophoresis-aided CPP-ACP with 1...
June 11, 2018: Scientific Reports
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