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https://www.readbyqxmd.com/read/27909432/volatile-mediated-effects-predominate-in-paraburkholderia-phytofirmans-growth-promotion-and-salt-stress-tolerance-of-arabidopsis-thaliana
#1
Thomas Ledger, Sandy Rojas, Tania Timmermann, Ignacio Pinedo, María J Poupin, Tatiana Garrido, Pablo Richter, Javier Tamayo, Raúl Donoso
Abiotic stress has a growing impact on plant growth and agricultural activity worldwide. Specific plant growth promoting rhizobacteria have been reported to stimulate growth and tolerance to abiotic stress in plants, and molecular mechanisms like phytohormone synthesis and 1-aminocyclopropane-1-carboxylate deamination are usual candidates proposed to mediate these bacterial effects. Paraburkholderia phytofirmans PsJN is able to promote growth of several plant hosts, and improve their tolerance to chilling, drought and salinity...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27909291/engineering-of-a-thermostable-esterase-est816-to-improve-its-quorum-quenching-activity-and-the-underlying-structural-basis
#2
Xiwen Liu, Li-Chuang Cao, Xin-Jiong Fan, Yu-Huan Liu, Wei Xie
N-acyl-homoserine lactones (AHLs) are small diffusible molecules called autoinducers that mediate cell-to-cell communications. Enzymatic degradation of AHLs is a promising bio-control strategy known as quorum-quenching. To improve the quorum-quenching activity of a thermostable esterase Est816, which had been previously cloned, we have engineered the enzyme by random mutagenesis. One of the mutants M2 with double amino acid substitutions (A216V/K238N) showed 3-fold improvement on catalytic efficiency. Based on the crystal structure determined at 2...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27900697/screening-for-n-ahsl-based-signaling-interfering-enzymes
#3
Stéphane Uroz, Phil M Oger
Quorum sensing (QS)-based signaling is a widespread pathway used by bacteria for the regulation of functions involved in their relation to the environment or their host. QS relies upon the production, accumulation and perception of small diffusable molecules by the bacterial population, hence linking high gene expression with high cell population densities. Among the different QS signal molecules, an important class of signal molecules is the N-acyl homoserine lactone (N-AHSL). In pathogens such as Erwinia or Pseudomonas, N-AHSL based QS is crucial to overcome the host defenses and ensure a successful infection...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27900439/non-ribosomal-peptide-synthases-from-pseudomonas-aeruginosa-play-a-role-in-cyclodipeptide-biosynthesis-quorum-sensing-regulation-and-root-development-in-a-plant-host
#4
Omar González, Randy Ortíz-Castro, César Díaz-Pérez, Alma L Díaz-Pérez, Viridiana Magaña-Dueñas, José López-Bucio, Jesús Campos-García
Diverse molecules mediate cross-kingdom communication between bacteria and their eukaryotic partners and determine pathogenic or symbiotic relationships. N-acyl-L-homoserine lactone-dependent quorum-sensing signaling represses the biosynthesis of bacterial cyclodipeptides (CDPs) that act as auxin signal mimics in the host plant Arabidopsis thaliana. In this work, we performed bioinformatics, biochemical, and plant growth analyses to identify non-ribosomal peptide synthase (NRPS) proteins of Pseudomonas aeruginosa, which are involved in CDP synthesis...
November 30, 2016: Microbial Ecology
https://www.readbyqxmd.com/read/27896004/photolysis-of-a-peptide-with-n-peptidyl-7-nitroindoline-units-using-two-photon-absorption
#5
Kevin A Hatch, Alfredo Ornelas, Kaitlyn N Williams, Thomas Boland, Katja Michael, Chunqiang Li
N-acyl-7-nitroindolines have been used as caged compounds to photorelease active molecules by a one- or two-photon excitation mechanism in biological systems. Here, we report the photolysis of a polypeptide that contains 7-nitroindoline units as linker moieties in its peptide backbone for potential materials engineering applications. Upon two-photon excitation with femtosecond laser light at 710 nm the photoreactive amide bond in N-peptidyl-7-nitroindolines is cleaved rendering short peptide fragments. Thus, this photochemical process changes the molecular composition at the laser focal volume...
November 1, 2016: Biomedical Optics Express
https://www.readbyqxmd.com/read/27893106/evidence-for-a-gpr18-role-in-diurnal-regulation-of-intraocular-pressure
#6
Sally Miller, Emma Leishman, Olivia Oehler, Laura Daily, Natalia Murataeva, Jim Wager-Miller, Heather Bradshaw, Alex Straiker
Purpose: The diurnal cycling of intraocular pressure (IOP) was first described in humans more than a century ago. This cycling is preserved in other species. The physiologic underpinning of this diurnal variation in IOP remains a mystery, even though elevated pressure is indicated in most forms of glaucoma, a common cause of blindness. Once identified, the system that underlies diurnal variation would represent a natural target for therapeutic intervention. Methods: Using normotensive mice, we measured the regulation of ocular lipid species by the enzymes fatty acid amide hydrolase (FAAH) and N-arachidonoyl phosphatidylethanolamine phospholipase (NAPE-PLD), mRNA expression of these enzymes, and their functional role in diurnal regulation of IOP...
November 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27881717/localization-of-1-deoxysphingolipids-to-mitochondria-induces-mitochondrial-dysfunction
#7
Irina Alecu, Andrea Tedeschi, Natascha Behler, Klaus Wunderling, Christian Lamberz, Mario A R Lauterbach, Anne Gaebler, Daniela Ernst, Paul P Van Veldhoven, Ashraf Al-Amoudi, Eicke Latz, Alaa Othman, Lars Kuerschner, Thorsten Hornemann, Frank Bradke, Christoph Thiele, Anke Penno
1-Deoxysphingolipids (deoxySLs) are atypical sphingolipids that are elevated in the plasma of patients with type 2 diabetes and hereditary sensory and autonomic neuropathy type 1 (HSAN1). Clinically, diabetic neuropathy and HSAN1 are very similar, suggesting the involvement of deoxySLs in the pathology of both diseases. However, very little is known about the biology of these lipids and the underlying pathomechanism. We synthesized an alkyne analogue of 1-deoxysphinganine (doxSA), the metabolic precursor of all deoxySLs, to trace the metabolism and localization of deoxySLs...
November 23, 2016: Journal of Lipid Research
https://www.readbyqxmd.com/read/27873164/bacterial-signals-n-acyl-homoserine-lactones-induce-the-changes-of-morphology-and-ethanol-tolerance-in-saccharomyces-cerevisiae
#8
Ge Ren, Anzhou Ma, Weifeng Liu, Xuliang Zhuang, Guoqiang Zhuang
The bacterial quorum sensing signals N-acyl homoserine lactone (AHL) signals are able to regulate a diverse array of physiological activities, such as symbiosis, virulence and biofilm formation, depending on population density. Recently, it has been discovered that the bacterial quorum sensing (QS) signal molecules can induce extensive response of higher eukaryotes including plants and mammalian cells. However, little is known about the response of fungi reacting to these bacterial signals. Here we showed that Saccharomyces cerevisiae, as an ancient eukaryote and widely used for alcoholic beverage and bioethanol production, exposed to short-chain 3-OC6-HSL and long-chain C12-HSL appeared obvious changes in morphology and ethanol tolerance...
December 2016: AMB Express
https://www.readbyqxmd.com/read/27863711/n-acylated-chitosan-bis-arylcarbamate-s-a-class-of-promising-chiral-separation-materials-with-powerful-enantioseparation-capability-and-high-eluents-tolerability
#9
Sheng Tang, Jian-De Liu, Qin Bin, Ke-Qin Fu, Xiao-Chen Wang, Ying-Bin Luo, Shao-Hua Huang, Zheng-Wu Bai
In order to comprehensively understand the influence of coordination of the substituent at 2-position with those at 3- and 6-positions on the properties of chitosan derivatives, a series of chitosan 3,6-bis(arylcarbamate)-2-(amide)s (CACAs) and the related chiral stationary phases (CSPs) were prepared and reported in the present study. Specifically, chitosan was N-acylated with carboxylic acid anhydrides, and then further derivatized with various aryl isocyanates to afford CACAs, from which a class of coated-type CSPs were prepared...
November 11, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27854038/biosynthesis-of-glycomonoterpenes-to-attenuate-quorum-sensing-associated-virulence-in-bacteria
#10
Amrita Patil, Kasturi Joshi-Navre, Ruchira Mukherji, Asmita Prabhune
The acquisition of multidrug resistance in bacteria has become a bigger threat of late, mainly due to the bacterial signaling phenomenon, quorum sensing (QS). QS, among a population of bacteria, initiates the formation of biofilms and offers myriad advantages to bacteria. Burgeoning antibiotic resistance in biofilm-producing bacteria has motivated efforts toward finding new alternatives to these traditional antimicrobials. In the present study, we report the increased solubility and additional quorum quenching as well as biofilm disruption activity of glyco-derivatives of monoterpenes (citral and citronellal)...
November 16, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27836537/possible-roles-of-long-chain-sphingomyelines-and-sphingomyelin-synthase-2-in-mouse-macrophage-inflammatory-response
#11
Hideaki Sakamoto, Tetsuya Yoshida, Takao Sanaki, Shuhei Shigaki, Hirotoshi Morita, Miki Oyama, Masaru Mitsui, Yoshikazu Tanaka, Toru Nakano, Susumu Mitsutake, Yasuyuki Igarashi, Hiroshi Takemoto
To evaluate the precise role of sphingomyelin synthase 2 (SMS2) in sphingomyelin (SM) metabolism and their anti-inflammatory properties, we analyzed species of major SM and ceramide (Cer) (18:1, 18:0 sphingoid backbone, C14 - C26 N-acyl part) in SMS2 knockout and wild-type mouse plasma and liver using HPLC-MS. SMS2 deficiency significantly decreased very long chain SM (SM (d18:1/22:0) and SM (d18:1/24:0 or d18:0/24:1)) and increased very long chain Cer (Cer (d18:1/24:0 or d18:0/24:1) and Cer (d18:1/24:1)), but not long chain SM (SM (d18:1/16:0), SM (d18:1/18:0 or d18:0/18:1) and SM (d18:1/18:1)) in plasma...
November 9, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27825531/preliminary-study-on-an-innovative-simple-mast-cell-based-electrochemical-method-for-detecting-foodborne-pathogenic-bacterial-quorum-signaling-molecules-n-acyl-homoserine-lactones
#12
Donglei Jiang, Dongdong Feng, Hui Jiang, Limin Yuan, Yin Yongqi, Xin Xu, Weiming Fang
This paper reports the a novel and simple mast cell-based electrochemical method for detecting of bacterial quorum signaling molecules, N-acylhomoserine lactones (AHLs), which can be utilized to preliminarily evaluate the toxicity of food-borne pathogenic bacteria. Rat basophilic leukemia (RBL-2H3) mast cells encapsulated in alginate/graphene oxide hydrogel were immobilized on a gold electrode, while mast cells as recognition elements were cultured in a 3D cell culture system. Electrochemical impedance spectroscopy (EIS) was utilized to record the cell impedance signal as-influenced by Pseudomonas aeruginosa quorum-sensing molecule, N-3-oxododecanoyl homoserine lactone (3OC12-HSL)...
September 28, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27814425/catalytic-enantioselective-acyl-transfer-the-case-for-4-ppy-with-a-c-3-carboxamide-peptide-auxiliary-based-on-synthesis-and-modelling-studies
#13
Rudy E Cozett, Gerhard A Venter, Maheswara Rao Gokada, Roger Hunter
A series of 4-pyrrolidinopyridine (4-PPY) C-3 carboxamides containing peptide-based side chains have been synthesised and evaluated in the kinetic resolution of a small library of chiral benzylic secondary alcohols. A key design element was the incorporation of a tryptophan residue in the peptide side chain for promoting π-stacking between peptide side chain and the pyridinium ring of the N-acyl intermediate, in which modelling was used as a structure-based guiding tool. Together, a catalyst containing a LeuTrp-N-Boc side chain (catalyst 8) was identified that achieved s-values up to and in slight excess of 10...
November 4, 2016: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/27784136/the-role-of-apolipoprotein-n-acyl-transferase-lnt-in-the-lipidation-of-factor-h-binding-protein-of-neisseria-meningitidis-strain-mc58-and-its-potential-as-a-drug-target
#14
Rag da Silva, C P Churchward, A V Karlyshev, O Eleftheriadou, A K Snabaitis, M R Longman, A Ryan, R Griffin
BACKGROUND AND PURPOSE: The level of cell surface expression of the meningococcal vaccine antigen, Factor H binding protein (FHbp) varies between and within strains and this limits the breadth of strains that can be targeted by FHbp-based vaccines. The molecular pathway dictating expression of FHbp at the cell surface, including its lipidation, sorting to the outer membrane and export, and the potential regulation of this pathway have not been investigated until now. This knowledge will aid our evaluation of FHbp vaccines...
October 26, 2016: British Journal of Pharmacology
https://www.readbyqxmd.com/read/27783481/solid-phase-parallel-synthesis-of-n-substituted-2-aminothiazolo-4-5-b-pyrazine-derivatives-via-tandem-reaction-of-isothiocyanate-terminated-resin-with-o-bromo-2-aminopyrazines
#15
Aizhan Abdildinova, Seung-Ju Yang, Young-Dae Gong
A novel solid-phase synthesis methodology of N-substituted-2-aminothiazolo[4,5-b]pyrazine derivatives was developed. The key step in this synthesis strategy is the tandem reaction of isothiocyanate terminated resin 2 with o-bromo-2-aminopyrazine, affording cyclized 2-aminothiazolo[4,5-b]pyrazine resin 4. To increase the diversity of our library, Suzuki coupling reaction was performed at the position C6. Further functionalization of 2-aminothiazolo[4,5-b]pyrazine core skeleton with various electrophiles such as alkyl halides, acyl chlorides, and sulfonyl chlorides and cleavage from the resin with TFA in DCM generated N-alkyl-, N-acyl-, and N-sulfonyl-2-aminothiazolo[4,5-b]pyrazine derivatives...
October 26, 2016: ACS Combinatorial Science
https://www.readbyqxmd.com/read/27777468/effect-of-small-chain-n-acyl-homoserine-lactone-quorum-sensing-signals-on-biofilms-of-food-borne-pathogens
#16
Jamuna Bai A, Ravishankar Rai V
Quorum sensing or cell to cell communication which includes inter- and intra-cellular communication has been implicated in the production of virulence factor and formation of biofilm in food-borne pathogens. In the present study, the effect of quorum sensing signals on the biofilms of food-borne pathogens has been elucidated. N-butryl homoserine lactone and N-hexanoyl homoserine lactone belonging to acyl homoserine lactone (AHL) family of signaling molecules were investigated for their effect on the biofilm formation (attachment and exopolymeric substance production) in the food-borne pathogens Escherichia coli, Salmonella enterica serovar Typhimurium and Vibrio parahemolyticus...
September 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/27771949/enantioselective-total-synthesis-of-hinckdentine-a-via-a-catalytic-dearomatization-approach
#17
Kazuya Douki, Hiroyuki Ono, Tohru Taniguchi, Jun Shimokawa, Masato Kitamura, Tohru Fukuyama
Optically pure hinckdentine A was synthesized on a 300 mg scale via an asymmetric catalysis-based strategy. The key steps to the first asymmetric synthesis involved i) enantioselective dearomative cyclization of an achiral N-acyl indole that allowed for the efficient construction of the key polycyclic indoline intermediate with a crucial tetrasubstituted stereogenic carbon center, ii) Beckmann fragmentation-mediated ring expansion, iii) rearrangement-based introduction of an anilinic nitrogen atom, iv) regioselective tribromination, and v) final closure of the cyclic amidine moiety...
October 22, 2016: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/27771218/structure-supramolecular-organization-and-phase-behavior-of-n-acyl-%C3%AE-alanines-structural-homologues-of-mammalian-brain-constituents-n-acylglycine-and-n-acyl-gaba
#18
D Sivaramakrishna, Musti J Swamy
N-Acyl-β-alanines (NABAs) are structural homologues of N-acylglycines (NAGs) and N-acyl-γ-aminobutyric acids (NAGABAs), and achiral isomers of N-acylalanines, which are all present in mammalian brain and other tissues and modulate activity of biological receptors with various functions. In the present study, we synthesized and characterized a homologous series of NABAs bearing saturated acyl chains (n=8-20) and investigated their supramolecular organization and thermotropic phase behavior. In differential scanning calorimetric (DSC) studies, most of the NABAs gave one or two minor transitions before the main chain-melting phase transition in the dry state as well as upon hydration with water, but gave only a single transition when hydrated with buffer (pH7...
October 19, 2016: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/27771051/enhanced-oral-delivery-of-bisphosphonate-by-novel-absorption-enhancers-improvement-of-intestinal-absorption-of-alendronate-by-n-acyl-amino-acids-and-n-acyl-taurates-and-their-absorption-enhancing-mechanisms
#19
Yuka Nakaya, Mayu Takaya, Yuta Hinatsu, Tammam Alama, Kosuke Kusamori, Hidemasa Katsumi, Toshiyasu Sakane, Akira Yamamoto
Bisphosphonates (BPs) are carbon-substituted pyrophosphate analogs that exhibit a high affinity to hydroxyapatite and specifically inhibit bone resorption. Alendronate sodium (sodium 4-amino-1-hydroxybutylidene-1,1-bisphosphonate trihydrate) is a typical BP compound in clinical use. BPs have very low bioavailability, typically <1% after their oral administration, and their intestinal absorption is further reduced by co-administered drugs or food. In this study, we examined the effects of N-acyl amino acids and N-acyl taurates on the small intestinal absorption of alendronate...
October 19, 2016: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27762045/exploiting-the-imidazolium-effect-in-base-free-ammonium-enolate-generation-synthetic-and-mechanistic-studies
#20
Claire M Young, Daniel G Stark, Thomas H West, James E Taylor, Andrew D Smith
N-Acyl imidazoles and catalytic isothiourea hydrochloride salts function as ammonium enolate precursors in the absence of base. Enantioselective Michael addition-cyclization reactions using different α,β-unsaturated Michael acceptors have been performed to form dihydropyranones and dihydropyridinones with high stereoselectivity. Detailed mechanistic studies using RPKA have revealed the importance of the "imidazolium" effect in ammonium enolate formation and have highlighted key differences with traditional base-mediated processes...
October 20, 2016: Angewandte Chemie
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