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NADH AND dopamine

Dian Wang, Haitao Yu, Benhong Xu, Hua Xu, Zaijun Zhang, Xiaohu Ren, Jianhui Yuan, Jianjun Liu, Yi Guo, Peter S Spencer, Xifei Yang
Transient receptor potential channel 1 (TRPC1) is widely expressed throughout the nervous system, while its biological role remains unclear. In this study, we showed that TRPC1 deletion caused striatal neuronal loss and significantly increased TUNEL-positive and 8-hydroxy-2'-deoxyguanosine (8-OHdG) staining in the striatum. Proteomic analysis by two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) coupled with mass spectrometry (MS) revealed a total of 51 differentially expressed proteins (26 increased and 25 decreased) in the stratum of TRPC1 knockout (TRPC1-/- ) mice compared to that of wild type (WT) mice...
2018: Frontiers in Aging Neuroscience
Denis E Bragin, Gloria L Statom, Edwin M Nemoto
OBJECTIVE: In previous work we showed that high intracranial pressure (ICP) in the rat brain induces a transition from capillary (CAP) to pathological microvascular shunt (MVS) flow, resulting in brain hypoxia, edema, and blood-brain barrier (BBB) damage. This transition was correlated with a loss of cerebral blood flow (CBF) autoregulation undetected by static autoregulatory curves but identified by induced dynamic ICP (iPRx) and cerebrovascular (iCVRx) reactivity. We hypothesized that loss of CBF autoregulation as correlated with MVS flow would be identified by iPRx and iCVRx in traumatic brain injury (TBI) with elevated ICP...
2018: Acta Neurochirurgica. Supplement
Yashuang Hei, Xiqian Li, Xiao Zhou, Jingju Liu, Mimi Sun, Tianze Sha, Cuixing Xu, Wanyi Xue, Xiangjie Bo, Ming Zhou
In this paper, kelp (Laminaria japonica), as a kind of abundant biomass, is used as the precursor for the preparation of kelp-derived hierarchical meso-macroporous carbons (K-dHMMCs) through the carbonization under nitrogen (N2 ) atmosphere at high temperature. The K-dHMMCs exhibits the unique structure with high specific surface area of 416.02 m2  g-1 , large pore volume of 0.24 cm3  g-1 , the hierarchical meso-macroporous size distribution centered at 2, 12 and 82 nm and high density of defective sites, enabling K-dHMMCs attractive for the electrocatalysis...
March 20, 2018: Analytica Chimica Acta
Sandeep Chakraborty, Artashes Karmenyan, Jin-Wu Tsai, Arthur Chiou
Development and progression of neurodegenerative diseases like Parkinson's disease (PD) involve multiple pathways. Thus, effective therapeutic treatments should intervene to address all these pathways simultaneously for greater success. Most of the current pharmacotherapeutic approaches just supplement striatal dopamine. Hence, natural extracts of plants with therapeutic potential have been explored. Curcuminoids belong to one such group of polyphenol which show immense therapeutic effects. Here, we have used intracellular reactive oxygen species (ROS) measurement, and two-photon fluorescence lifetime imaging microscopy (2P-FLIM) of cellular autofluorescent co-enzyme reduced nicotinamide adenine dinucleotide (NADH) to study the inhibitory effects of curcumin and cyclocurcumin in alleviating PD like neurotoxicity of 1-methyl-4-phenylpyridinium (MPP+ ) in neuronal growth factor (NGF) induced differentiated PC12 cells...
December 5, 2017: Scientific Reports
Wei-Hai Chen, Margarita Vázquez-González, Anna Kozell, Alessandro Cecconello, Itamar Willner
The synthesis and characterization of UiO-type metal-organic framework nanoparticles (NMOFs) composed of Zr4+ ions bridged by 2,2'-bipyridine-5,5'-dicarboxylic acid ligands and the postmodification of the NMOFs with Cu2+ ions are described. The resulting Cu2+ -modified NMOFs, Cu2+ -NMOFs, exhibit peroxidase-like catalytic activities reflected by the catalyzed oxidation of Amplex-Red to the fluorescent Resorufin by H2 O2 , the catalyzed oxidation of dopamine to aminochrome by H2 O2 , and the catalyzed generation of chemiluminescence in the presence of luminol/H2 O2 ...
February 2018: Small
D Sangamithirai, S Munusamy, V Narayanan, A Stephen
A study on the voltammetric detection of NADH (β-nicotinamide adenine dinucleotide), Dopamine (DA) and their simultaneous determination is presented in this work. The electrochemical sensor was fabricated with the hybrid nanocomposites of poly(o-anisidine) and silver nanoparticles prepared by simple and cost-effective insitu chemical oxidative polymerization technique. The nanocomposites were synthesized with different (w/w) ratios of o-anisidine and silver by increasing the amount of o-anisidine in each, by keeping silver at a fixed quantity...
November 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Margarita Vázquez-González, Rebeca M Torrente-Rodríguez, Anna Kozell, Wei-Ching Liao, Alessandro Cecconello, Susana Campuzano, José M Pingarrón, Itamar Willner
Nanoparticles composed of Prussian Blue, PB, and the cyanometalate structural analogues, CuFe, FeCoFe, and FeCo, are examined as inorganic clusters that mimic the functions of peroxidases. PB acts as a superior catalyst for the oxidation of dopamine to aminochrome by H2O2. The oxidation of dopamine by H2O2 in the presence of PB is 6-fold faster than in the presence of CuFe. The cluster FeCo does not catalyze the oxidation of dopamine to aminochrome. The most efficient catalyst for the generation of chemiluminescence by the oxidation of luminol by H2O2 is, however, FeCo, and PB lacks any catalytic activity toward the generation of chemiluminescence...
July 10, 2017: Nano Letters
Shan Wang, Rémi Cazelles, Wei-Ching Liao, Margarita Vázquez-González, Amani Zoabi, Raed Abu-Reziq, Itamar Willner
Cu(2+)-ion-modified graphene oxide nanoparticles, Cu(2+)-GO NPs, act as a heterogeneous catalyst mimicking functions of horseradish peroxidase, HRP, and of NADH peroxidase. The Cu(2+)-GO NPs catalyze the oxidation of dopamine to aminochrome by H2O2 and catalyze the generation of chemiluminescence in the presence of luminol and H2O2. The Cu(2+)-GO NPs provide an active material for the chemiluminescence detection of H2O2 and allow the probing of the activity of H2O2-generating oxidases and the detection of their substrates...
March 8, 2017: Nano Letters
Kristal R Tucker, Samantha L Cavolo, Edwin S Levitan
Pyridine nucleotides are redox coenzymes that are critical in bioenergetics, metabolism, and neurodegeneration. Here we use brain slice multiphoton microscopy to show that substantia nigra dopamine neurons, which are sensitive to stress in mitochondria and the endoplasmic reticulum (ER), display elevated combined NADH and NADPH (i.e., NAD(P)H) autofluorescence. Despite limited mitochondrial mass, organellar NAD(P)H is extensive because much of the signal is derived from the ER. Remarkably, even though pyridine nucleotides cannot cross mitochondrial and ER membranes, inhibiting mitochondrial function with an uncoupler or interrupting the electron transport chain with cyanide (CN(-)) alters ER NAD(P)H...
November 1, 2016: Molecular Biology of the Cell
Shanying Han, Tianyu Du, Hui Jiang, Xuemei Wang
A self-assembly composite of graphene-pyrroloquinoline quinone (PQQ) was fabricated and modified on glassy carbon electrode (GCE) for sensitive detection of nicotinamide adenine dinucleotide (NADH). Chitosan (CTS) was applied to disperse graphene to form a stable robust film on GCE. A synergistic effect between PQQ and graphene was observed during the electrocatalytic oxidation of NADH, with about 260mV reduction in the oxidation potential and 2.5-fold increase in the oxidation current compared with those on the bare GCE...
March 15, 2017: Biosensors & Bioelectronics
Jayaraman Balamurugan, Tran Duy Thanh, Nam Hoon Kim, Joong Hee Lee
Herein, we present a novel strategy for the synthesis of an iron nitride nanoparticles-encapsulated nitrogen-doped graphene (FeN NPs/NG) core-shell hierarchical nanostructure to boost the electrochemical performance in a highly sensitive, selective, reproducible, and stable sensing platform for nicotinamide adenine dinucleotide (NADH). This core-shell hierarchical nanostructure provides an excellent conductive network for effective charge transfer and avoids the agglomeration and restacking of NG sheets, which provides better access to the electrode material for NADH oxidation...
September 15, 2016: Biosensors & Bioelectronics
Veronika Urbanová, František Karlický, Adam Matěj, Filip Šembera, Zbyněk Janoušek, Jason A Perman, Václav Ranc, Klára Čépe, Josef Michl, Michal Otyepka, Radek Zbořil
Graphene derivatives are promising materials for the electrochemical sensing of diverse biomolecules and development of new biosensors owing to their improved electron transfer kinetics compared to pristine graphene. Here, we report complex electrochemical behavior and electrocatalytic performance of variously fluorinated graphene derivatives prepared by reaction of graphene with a nitrogen-fluorine mixture at 2 bars pressure. The fluorine content was simply controlled by varying the reaction time and temperature...
June 16, 2016: Nanoscale
Danúbia Bonfanti Santos, Dirleise Colle, Eduardo Luiz Gasnhar Moreira, Mariana Appel Hort, Marcelo Godoi, Gael Le Douaron, Antonio Luiz Braga, Jamil Assreuy, Patrick Pierre Michel, Rui Daniel Prediger, Rita Raisman-Vozari, Marcelo Farina
Parkinson's disease (PD) is a neurodegenerative disorder characterized by non-motor and motor disabilities. This study investigated whether succinobucol (SUC) could mitigate nigrostriatal injury caused by intranasal 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administration in mice. Moreover, the effects of SUC against MPTP-induced behavioral impairments and neurochemical changes were also evaluated. The quantification of tyrosine hydroxylase-positive (TH+ ) cells was also performed in primary mesencephalic cultures to evaluate the effects of SUC against 1-methyl-4-phenylpyridinium (MPP+ ) toxicity in vitro...
March 2017: Molecular Neurobiology
Fatin Saiha Omar, Navaneethan Duraisamy, K Ramesh, S Ramesh
Nicotinamide Adenine Dinucleotide (NADH) is an important coenzyme in the human body that participates in many metabolic reactions. The impact of abnormal concentrations of NADH significantly causes different diseases in human body. Electrochemical detection of NADH using bare electrode is a challenging task especially in the presence of main electroactive interferences such as ascorbic acid (AA), uric acid (UA) and dopamine (DA). Modified electrodes have been widely explored to overcome the problems of poor sensitivity and selectivity occurred from bare electrodes...
May 15, 2016: Biosensors & Bioelectronics
Sriranjini Venkat Rao, Muralidhara, Sarat Chandra Yenisetti, Padmanabhan S Rajini
Evidence suggests that saffron and its major bioactives exhibit significant neuromodulatory effects in various animal models. However, specific data related to their efficacy to attenuate oxidative stress and neurotoxicity in animal models of Parkinson's disease (PD) are limited. Hence, we investigated the neuroprotective efficacy of saffron methanolic extract (SME) and its active constituent, crocin (CR) employing a Drosophila model of parkinsonism. We focussed on attenuation of Rotenone (ROT)-induced locomotor phenotype, oxidative stress, mitochondrial dysfunction and neurotoxicity in this model...
January 2016: Neurotoxicology
Jayshree Nellore, Cynthia Pauline, Kanchana Amarnath
Current discovery demonstrates the rapid formation of platinum nanoparticles using leaf extract of a neurobeneficial plant, Bacopa monnieri (BmE). The nanoparticles (BmE-PtNPs) were stabilized and then coated with varied phytochemicals present within the leaf extract. These nanoparticles demonstrated the same activity of Complex I, as that of oxidizing NADH to NAD(+) using a spectrophotometric method. This suggests that BmE-PtNPs are a potential medicinal substance for oxidative stress mediated disease with suppressed mitochondrial complex I, namely, Parkinson's disease (PD)...
2013: Journal of Neurodegenerative Diseases
M J Manjunath, Muralidhara
Withania somnifera (Ashwagandha, WS) or Indian ginseng possesses multiple pharmacological properties which are mainly attributed to the active constituents, withanolides. Despite its extensive usage as a memory enhancer and a nerve tonic, few attempts have been made to ascertain its usage in the management of Parkinson's disease. In the present study, we investigated the neuroameliorative effects of WS in a rotenone (ROT) model of Drosophila melanogaster (Oregon-K). Initially, we ascertained the ability of WS-enriched diet (0-0...
April 2015: Journal of Food Science and Technology
Chandramohan Wakade, Raymond Chong
We hypothesize that GPR109A message and expression are up-regulated in individuals with Parkinson's disease (PD). GPR109A is a high-affinity niacin receptor. Niacin is a precursor for NAD-NADH which is needed for dopamine production. Thus, niacin supplementation may serve three purposes: reduce inflammation through GPR109A-related mechanisms, increase dopamine synthesis in the striatum through NADPH supply and increase NAD/NADH ratio to boost mitochondrial functions. GPR109A and its agonists are known to exert anti-inflammatory actions in the skin, gut and retina...
December 15, 2014: Journal of the Neurological Sciences
Janete Quelhas-Santos, Maria Paula Serrão, Isabel Soares-Silva, Cátia Fernandes-Cerqueira, Liliana Simões-Silva, Maria João Pinho, Fernando Remião, Benedita Sampaio-Maia, Gary V Desir, Manuel Pestana
Renalase is a recently identified FAD/NADH-dependent amine oxidase mainly expressed in kidney that is secreted into blood and urine where it was suggested to metabolize catecholamines. The present study evaluated central and peripheral dopaminergic activities in the renalase knockout (KO) mouse model and examined the changes induced by recombinant renalase (RR) administration on plasma and urine catecholamine levels. Compared with wild-type (WT) mice, KO mice presented increased plasma levels of epinephrine (Epi), norepinephrine (NE), and dopamine (DA) that were accompanied by increases in the urinary excretion of Epi, NE, DA...
January 15, 2015: American Journal of Physiology. Renal Physiology
Somen Mondal, Manjunath Chatti, Arabinda Mallick, Pradipta Purkayastha
Dopamine functionalized carbon nanoparticles (CNPs) that can act as efficient photoinduced electron donor-acceptor systems depending on the pH of the medium have been synthesized. In acidic media, dopamine on CNPs exists as hydroquinone and serves as an electron donor while under alkaline conditions the corresponding quinone form of dopamine serves as a strong electron acceptor. Application of external NADH to the system can invert the donor-acceptor roles under alkaline conditions.
July 4, 2014: Chemical Communications: Chem Comm
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