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long non coded rna mrna

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https://www.readbyqxmd.com/read/28919047/long-non-coding-rna-657-suppresses-hepatocellular-carcinoma-cell-growth-by-acting-as-a-molecular-sponge-of-mir-106a-5p-to-regulate-pten-expression
#1
Bingren Hu, Huajie Cai, Ru Zheng, Shouzhang Yang, Zhenxu Zhou, Jinfu Tu
Previous study has identified the aberrant expression of LINC00657, a long non-coding RNA (lncRNA), in human breast cancer. However, the expression pattern, biological function and underlying mechanism of LINC00657 in human hepatocellular carcinoma (HCC) remain obscure. The expression levels of LINC00657 in HCC tissues and cell lines were determined by quantitative real-time PCR. CCK-8 assay, cell colony formation assay, cell cycle analysis, Transwell assay were performed to determine whether LINC00657 could affect HCC progression...
September 14, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28916736/silencing-of-urothelial-carcinoma-associated-1-inhibits-the-proliferation-and-migration-of-medulloblastoma-cells
#2
Xie Zhengyuan, Xiao Hu, Wang Qiang, Li Nanxiang, Cai Junbin, Zhang Wangming
BACKGROUND UCA1 is a long non-coding RNA that has been found to be aberrantly upregulated in various cancers. The aim of this study was to determine the expression level and function of UCA1 in medulloblastoma, the most common malignant brain tumor during childhood. MATERIAL AND METHODS Real-time PCR was used to detect the expression of UCA1 in medulloblastoma specimens and cell lines. Lentiviral-mediated expression of a short hairpin RNA (shRNA) targeting UCA1 or a negative control shRNA was also achieved with the medulloblastoma cell line, Daoy...
September 16, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28915634/dna-methylation-signature-of-long-noncoding-rna-genes-during-human-pre-implantation-embryonic-development
#3
Jingyu Li, Wei Han, Xiaoli Shen, Shubiao Han, Hong Ye, Guoning Huang
DNA methylation have crucial roles in regulating the expression of developmental genes during mammalian pre-implantation embryonic development (PED). However, the DNA methylation dynamic pattern of long noncoding RNA (lncRNA) genes, one type of epigenetic regulators, in human PED have not yet been demonstrated. Here, we performed a comprehensive analysis of lncRNA genes in human PED based on public reduced representation bisulphite sequencing (RRBS) data. We observed that both lncRNA and protein-coding genes complete the major demethylation wave at the 2-cell stage, whereas the promoters of lncRNA genes show higher methylation level than protein-coding genes during PED...
August 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28915561/differentially-expressed-lncrnas-and-mirnas-with-associated-cerna-networks-in-aged-mice-with-postoperative-cognitive-dysfunction
#4
Changwei Wei, Ting Luo, Shanshan Zou, Xiaobin Zhou, Wenzhen Shen, Xiaolin Ji, Qi Li, Anshi Wu
Postoperative cognitive dysfunction (POCD) is a common postoperative complication observed in elderly patients. Using microarray analyses, we comprehensively compared long non-coding RNA (lncRNA), messenger RNA (mRNA), and microRNA (miRNA) expression profiles in hippocampal tissues from a mouse model of POCD and control mice. A total of 175 lncRNAs, 117 mRNAs, and 26 miRNAs were differentially expressed between POCD and control mice. Gene ontology (GO) and KEGG pathway enrichment analyses were performed to explore the principal functions of dysregulated genes...
August 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28910376/long-non-coding-rna-set-and-mir-155-regulate-the-tnf%C3%AE-gene-allelic-expression-profile
#5
Chrysoula Stathopoulou, Manouela Kapsetaki, Kalliopi Stratigi, Charalampos Spilianakis
It is becoming increasingly appreciated that the non-coding genome may have a great impact on the regulation of chromatin structure and gene expression. The innate immune response can be mediated upon lipopolysaccharide stimulation of macrophages which leads to immediate transcriptional activation of early responsive genes including tumor necrosis factor alpha (Tnfα). The functional role of non-coding RNAs, such as lncRNAs and microRNAs, on the transcriptional activation of proinflammatory genes and the subsequent regulation of the innate immune response is still lacking mechanistic insights...
2017: PloS One
https://www.readbyqxmd.com/read/28881680/identification-of-dysregulated-long-non-coding-rnas-micrornas-mrnas-in-tnm-i-stage-lung-adenocarcinoma
#6
Ziqiang Tian, Shiwang Wen, Yuefeng Zhang, Xinqiang Shi, Yonggang Zhu, Yanzhao Xu, Huilai Lv, Guiying Wang
Lung adenocarcinoma (LUAD) is the primary subtype in lung cancer, which is the leading cause of cancer-related death worldwide. This study aimed to investigate the aberrant expression profiling of long non-coding RNA (lncRNA) in TNM I stage (stage I) LUAD. The lncRNA/mRNA/miRNA expression profiling of stage I LUAD and adjacent non-tumor tissues from 4 patients were measured by RNA-sequencing. Total of 175 differentially expressed lncRNAs (DELs), 1321 differentially expressed mRNAs (DEMs) and 94 differentially expressed microRNAs (DEMIs) were identified in stage I LUAD...
August 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28881636/the-landscape-of-dna-methylation-mediated-regulation-of-long-non-coding-rnas-in-breast-cancer
#7
Chunlong Zhang, Xinyu Wang, Xuecang Li, Ning Zhao, Yihan Wang, Xiaole Han, Ce Ci, Jian Zhang, Meng Li, Yan Zhang
Although systematic studies have identified a host of long non-coding RNAs (lncRNAs) which are involved in breast cancer, the knowledge about the methyla-tion-mediated dysregulation of those lncRNAs remains limited. Here, we integrated multi-omics data to analyze the methylated alteration of lncRNAs in breast invasive carcinoma (BRCA). We found that lncRNAs showed diverse methylation patterns on promoter regions in BRCA. LncRNAs were divided into two categories and four subcategories based on their promoter methylation patterns and expression levels be-tween tumor and normal samples...
August 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28873379/a-novel-long-non-coding-rna-sox21-as1-indicates-a-poor-prognosis-and-promotes-lung-adenocarcinoma-proliferation
#8
Xiyi Lu, Chenjun Huang, Xuezhi He, Xinyin Liu, Jianmei Ji, Erbao Zhang, Wei Wang, Renhua Guo
BACKGROUND: In recent years, long non-coding RNAs (lncRNAs) have been shown to be a novel class of regulators of cancer biological processes. Although lncRNAs are dysregulated in numerous cancer types, limited data are available on the expression profiles and potential functions of lncRNAs in lung adenocarcinoma (LUAD). This study evaluated the expression and biological roles of lncRNA SOX21 antisense RNA 1 (SOX21-AS1) in LUAD. METHODS: Quantitative reverse transcription PCR (qRT-PCR) was performed to detect the expression levels of SOX21-AS1 in 68 pairs of LUAD tissues and corresponding non-tumor tissues...
July 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28857668/malat1-mir-124-capn4-axis-regulates-proliferation-invasion-and-emt-in-nasopharyngeal-carcinoma-cells
#9
Baoyuan Shi, Yandan Wang, Fengfang Yin
BACKGROUND: Long non-coding RNA MALAT1 (Metastasis-associated lung Adenocarcinoma transcript-1) has been demonstrated to play a critical role in the regulation of cancer progression and metastasis. However, little is known about MALAT1 in nasopharyngeal carcinoma (NPC) pathogenesis and progression. METHODS: Quantitative real-time PCR (qRT-PCR) was conducted to measure the expression of MALAT1, miR-124 and Capn4 mRNA in NPC cell lines. The protein level of Capn4 was examined by western blot analysis...
August 31, 2017: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/28854977/up-regulation-of-lncrna-casc9-promotes-esophageal-squamous-cell-carcinoma-growth-by-negatively-regulating-pdcd4-expression-through-ezh2
#10
Yuanyuan Wu, Liwen Hu, Yan Liang, Juan Li, Kai Wang, Xuedan Chen, Hui Meng, Xingying Guan, Kang Yang, Yun Bai
BACKGROUND: Abnormal expression of numerous long non-coding RNAs (lncRNAs) has been reported in esophageal squamous cell carcinoma (ESCC) recently, but the great majority of their roles and mechanisms remain largely unclear. We aim to identify the critical ESCC-associated lncRNAs and elucidate the functions and mechanisms in detail. METHODS: Microarrays were used to analyze the differentially expressed lncRNAs in ESCC tissues. qRT-PCR was used to verify the result of microarrays...
August 30, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28854101/analyses-of-long-non-coding-rna-and-mrna-profiles-in-the-spinal-cord-of-rats-using-rna-sequencing-during-the-progression-of-neuropathic-pain-in-an-sni-model
#11
Jun Zhou, Youling Fan, Hongtao Chen
The pathogenesis of neuropathic pain (NP) is characterized by an increased responsiveness of nociceptive neurons in the nervous system. However, the molecular mechanisms underpinning the NP still remain elusive. Recent data suggest that long non-coding RNAs (lncRNAs) regulate expression of NP-associated genes. Herein, we analyzed lncRNAs and mRNA profiles in the spinal cord of rats by RNA sequencing during the progression of NP in a spared nerve injury (SNI) model. Sprague-Dawley (SD) rats were employed for the establishment of the SNI models, and nociceptive responses to mechanical and thermal stimuli were measured 3 hours prior to surgery and on postoperative days 1, 3, 7 and 14, with L [4-5] spinal cords extracted from three SD rats under deep anesthesia at each time point after behavioral test...
August 30, 2017: RNA Biology
https://www.readbyqxmd.com/read/28844995/-expression-profile-of-long-non-coding-rna-in-non-alcoholic-fatty-liver-disease
#12
Chuanzheng Sun, Feizhou Huang, Wenguang Yan, Huaizheng Liu, Changfa Wang
To analyze the characteristics of long non-coding RNA (lncRNA) expression in non-alcoholic fatty liver disease (NAFLD).
 Methods: lncRNA-mRNA microarray was conducted on the liver tissue samples from 10 patients with simple gallbladder stone (5 NAFLD liver samples and 5 normal liver samples), and the differentially expressed lncRNA was analyzed by bioinformatics technology.
 Results: Compared with the normal liver samples, there were abnormal expression of 1 735 lncRNAs and 1 485 mRNAs in NAFLD liver samples...
July 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28839203/fine-particulate-matter-2-5-exerted-its-toxicological-effect-by-regulating-a-new-layer-long-non-coding-rna
#13
Qiansheng Huang, Yulang Chi, Junjun Deng, Yiyao Liu, Yanyang Lu, Jinsheng Chen, Sijun Dong
Fine particulate matter (PM2.5) exposure, especially to its organic components, induces adverse health effects on the respiratory system. However, the molecular mechanisms have still not been fully elucidated. Long non-coding RNA (lncRNA) is involved in various physio-pathological processes. In this study, the roles of lncRNA were investigated to reveal the toxicology of PM2.5. Organic extracts of PM2.5 from Nanjing and Shanghai cities were adopted to treat human bronchial epithelial cell lines (BEAS-2B and A549)...
August 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28834756/regulation-of-peripheral-myelination-through-transcriptional-buffering-of-egr2-by-an-antisense-long-non-coding-rna
#14
Margot Martinez-Moreno, Timothy Mark O'Shea, John P Zepecki, Alexander Olaru, Jennifer K Ness, Robert Langer, Nikos Tapinos
Precise regulation of Egr2 transcription is fundamentally important to the control of peripheral myelination. Here, we describe a long non-coding RNA antisense to the promoter of Egr2 (Egr2-AS-RNA). During peripheral nerve injury, the expression of Egr2-AS-RNA is increased and correlates with decreased Egr2 transcript and protein levels. Ectopic expression of Egr2-AS-RNA in dorsal root ganglion (DRG) cultures inhibits the expression of Egr2 mRNA and induces demyelination. In vivo inhibition of Egr2-AS-RNA using oligonucleotide GapMers released from a biodegradable hydrogel following sciatic nerve injury reverts the EGR2-mediated gene expression profile and significantly delays demyelination...
August 22, 2017: Cell Reports
https://www.readbyqxmd.com/read/28831078/analyses-of-long-non-coding-rna-and-mrna-profiling-using-rna-sequencing-in-chicken-testis-with-extreme-sperm-motility
#15
Yifan Liu, Yanyan Sun, Yunlei Li, Hao Bai, Fuguang Xue, Songshan Xu, Hong Xu, Lei Shi, Ning Yang, Jilan Chen
Sperm motility is the most important indicator in evaluating roosters' fecundity. However, the genetic mechanisms underlying chicken sperm motility is not yet clear. Long non-coding RNA (lncRNA) play epigenetic roles in reproduction. In this study, RNA sequencing was employed to profile the testis transcriptome (lncRNA and mRNA) of six Beijing-you cocks divergent in sperm motility. In total, 2,597 lncRNAs were identified in the chicken testis, including 1,267 lincRNAs, 975 anti-sense lncRNAs, and 355 intronic lncRNAs...
August 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28819405/linc00152-promotes-cancer-cell-proliferation-and-invasion-and-predicts-poor-prognosis-in-lung-adenocarcinoma
#16
Pei-Pei Zhang, Yi-Qin Wang, Wei-Wei Weng, Wei Nie, Yong Wu, Yu Deng, Ping Wei, Mi-Die Xu, Chao-Fu Wang
Background: The long non-coding RNA Linc00152 stimulates tumor progression in cancer. However, its clinical significance and biological functions in lung adenocarcinoma remains unknown. We evaluate the expression of Linc00152 in lung adenocarcinoma and its possible correlation with clinicopathologic features and patient survival to reveal its biological effects in cancer progression and prognosis. Methods: Total RNA extraction was performed on 110 pairs of lung adenocarcinoma and adjacent normal tissue samples, and then RT-qPCR was conducted...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28815726/differential-expression-profile-analysis-of-lncrna-uca1%C3%AE-regulated-mrnas-in-bladder-cancer
#17
Yu Wang, Hong Zhang, Xu Li, Wei Chen
Urothelial carcinoma associated 1α (UCA1α) is a novel long non-coding RNA (lncRNA) that regulates bladder cancer proliferation, migration, and invasion. The target genes of UCA1α have, however, not been identified. To address this, a pCDNA3.1(+)-UCA1α over-expression vector was transfected into UM-UC-2 bladder cancer cells. Genes differentially expressed between pCDNA3.1(+)-UCA1α and pCDNA3.1(+) transfected cell were then detected by microarray and bioinformatics analysis. A total of seventy-one differentially expressed genes were identified, including fifty-two up-regulated genes and nineteen down-regulated genes...
August 16, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28810905/omni-polya-a-method-and-tool-for-accurate-recognition-of-poly-a-signals-in-human-genomic-dna
#18
Arturo Magana-Mora, Manal Kalkatawi, Vladimir B Bajic
BACKGROUND: Polyadenylation is a critical stage of RNA processing during the formation of mature mRNA, and is present in most of the known eukaryote protein-coding transcripts and many long non-coding RNAs. The correct identification of poly(A) signals (PAS) not only helps to elucidate the 3'-end genomic boundaries of a transcribed DNA region and gene regulatory mechanisms but also gives insight into the multiple transcript isoforms resulting from alternative PAS. Although progress has been made in the in-silico prediction of genomic signals, the recognition of PAS in DNA genomic sequences remains a challenge...
August 15, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28793054/revealing-potential-long-non-coding-rna-biomarkers-in-lung-adenocarcinoma-using-long-non-coding-rna-mediated-competitive-endogenous-rna-network
#19
T-G Zhu, X Xiao, Q Wei, M Yue, L-X Zhang
In our study, we aimed to reveal potential long non-coding RNAs (lncRNA) biomarkers in lung adenocarcinoma (LAD) using lncRNA-mediated competing endogenous RNAs (ceRNAs) network (LMCN). Competing lncRNA-mRNA interactions were identified using the hypergeometric test. Co-expression analysis for the competing lncRNA-mRNA interactions was implemented, and relying on the weight value >0.8, a highly competitive LMCN was further constructed. Degree distribution, betweenness and closeness for LMCN were carried out to analyze the network structure...
August 7, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28790843/comprehensive-analysis-of-differential-co-expression-patterns-reveal-transcriptional-dysregulation-mechanism-and-identify-novel-prognostic-lncrnas-in-esophageal-squamous-cell-carcinoma
#20
Zhen Li, Qianlan Yao, Songjian Zhao, Yin Wang, Yixue Li, Zhen Wang
Esophageal squamous cell carcinoma (ESCC) is one of the most common malignancies worldwide and occurs at a relatively high frequency in People's Republic of China. However, the molecular mechanism underlying ESCC is still unclear. In this study, the mRNA and long non-coding RNA (lncRNA) expression profiles of ESCC were downloaded from the Gene Expression Omnibus database, and then differential co-expression analysis was used to reveal the altered co-expression relationship of gene pairs in ESCC tumors. A total of 3,709 mRNAs and 923 lncRNAs were differentially co-expressed between normal and tumor tissues, and we found that most of the gene pairs lost associations in the tumor tissues...
2017: OncoTargets and Therapy
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