keyword
https://read.qxmd.com/read/31607702/biological-tailoring-of-adjuvant-radiotherapy-in-head-and-neck-and-oral-malignancies-the-potential-role-of-p53-and-eif4e-as-predictive-parameters
#21
JOURNAL ARTICLE
Bindhu Joseph, Rekha V Kumar, G Champaka, Ashok Shenoy, K S Sabitha, V Lokesh, C Ramesh, C R Vijay
BACKGROUND: Recent advances in radiation technology has allowed to significantly reduce toxicity and improve the efficacy of radical radiotherapy in head and neck and oral squamous cell cancers. Insights into molecular biology of carcinogenesis has opened a window for identifying aggressive clinical situations that may benefit with larger clinical target volume (CTV ) margin, broader levels of nodal coverage, or alternative radiation sensitizers. AIM: To evaluate the potential role of eukaryotic translation initiation factor 4E (elF4E) and p53 as predictive biomarkers in resected margins of head and neck and oral cancers...
October 2019: Indian Journal of Cancer
https://read.qxmd.com/read/31280681/prospects-for-potato-genome-editing-to-engineer-resistance-against-viruses-and-cold-induced-sweetening
#22
REVIEW
Amir Hameed, Muhammad Aamer Mehmood, Muhammad Shahid, Shabih Fatma, Aysha Khan, Sumbal Ali
Crop improvement through transgenic technologies is commonly tagged with GMO (genetically-modified-organisms) where the presence of transgene becomes a big question for the society and the legislation authorities. However, new plant breeding techniques like CRISPR/Cas9 system [clustered regularly interspaced palindromic repeats (CRISPR)-associated 9] can overcome these limitations through transgene-free products. Potato ( Solanum tuberosum L.) being a major food crop has the potential to feed the rising world population...
October 1, 2020: GM Crops & Food
https://read.qxmd.com/read/31191580/luciferase-based-screen-for-post-translational-control-factors-in-the-regulation-of-the-pseudo-response-regulator-prr7
#23
JOURNAL ARTICLE
Yeon Jeong Kim, David E Somers
Control of protein turnover is a key post-translational control point in the oscillatory network of the circadian clock. Some elements, such as TOC1 and PRR5 are engaged by a well-described F-box protein, ZEITLUPE, dedicated to their proteolytic turnover to shape their expression profile to a specific time of night. For most other clock components the regulation of their protein abundance is unknown, though turnover is often rapid and often lags the cycling of the respective mRNA. Here we report the design and results of an unbiased genetic screen in Arabidopsis to uncover proteolytic regulatory factors of PSEUDO-RESPONSE REGULATOR 7 (PRR7), a transcriptional repressor that peaks in the late afternoon...
2019: Frontiers in Plant Science
https://read.qxmd.com/read/31060678/-proliferation-of-microrna-365-and-e74-like-factor-4-in-cervical-cancer-cells-and-its-clinical-significance
#24
JOURNAL ARTICLE
Ying Guo, Dong Ma, Shi Feng Jia, Jia Liu, Shao Bei Fan, Meng Zhang, Lin Ran Shi, Li Li Jiang, Jue Xin Shi, Hai Qiu Wang, Huan Yu Zheng, Ou Li
Objective To investigate the expressions,roles,and clinical significance of microRNA-365(miR-365)and E74-like factor 4(ELF4)in cervical cancer. Methods The expressions of miR-365 in normal cervical tissues(n=34),cervical intraepithelial neoplasia 1(CIN 1)(n=31),cervical intraepithelial neoplasia2-3(CIN 2-3)(n=37),squamous cell carcinoma of the cervix(SCC)(n=33),and three cervical cancer cell lines(C33A cells,Hela cells,and SiHa cells)were detected by real-time quantitative polymerase chain reaction(qPCR).Bioinformatic prediction and luciferase reporter gene assay were performed to verify whether ELF4 was a direct target of miR-365...
April 28, 2019: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
https://read.qxmd.com/read/30917931/arabidopsis-elf4-like-proteins-efl1-and-efl3-influence-flowering-time
#25
JOURNAL ARTICLE
Ke Lin, Hang Zhao, Shuo Gan, Gang Li
The circadian clock synchronizes internal and external stimuli to ensure numerous biological processes occur at the optimal time. EARLY FLOWERING 4 (ELF4) is a key evening-phased component of the circadian clock and essential for photoperiod-dependent flowering time regulation in Arabidopsis thaliana. There are four homologous ELF4-like (EFL1-EFL4) genes in the Arabidopsis genome but their functions are unknown. Protein sequence alignment and phylogenetic analysis showed that these four EFL proteins contained an evolutionarily conserved domain, DUF1313, of unknown function...
June 5, 2019: Gene
https://read.qxmd.com/read/30898878/elf4-facilitates-innate-host-defenses-against-plasmodium-by-activating-transcription-of-pf4-and-ppbp
#26
JOURNAL ARTICLE
Dandan Wang, Zeming Zhang, Shuang Cui, Yingchi Zhao, Samuel Craft, Erol Fikrig, Fuping You
Platelet factor 4 (PF4) is an anti- Plasmodium component of platelets. It is expressed in megakaryocytes and released from platelets following infection with Plasmodium Innate immunity is crucial for the host anti- Plasmodium response, in which type I interferon plays an important role. Whether there is cross-talk between innate immune signaling and the production of anti- Plasmodium defense peptides is unknown. Here we demonstrate that E74, like ETS transcription factor 4 (ELF4), a type I interferon activator, can help protect the host from Plasmodium yoelii infection...
May 10, 2019: Journal of Biological Chemistry
https://read.qxmd.com/read/30890658/hipk2-is-necessary-for-type-i-interferon-mediated-antiviral-immunity
#27
JOURNAL ARTICLE
Lili Cao, Guang Yang, Shandian Gao, Chunxia Jing, Ruth R Montgomery, Yuxin Yin, Penghua Wang, Erol Fikrig, Fuping You
Precise control of interferons (IFNs) is crucial to maintain immune homeostasis. Here, we demonstrated that homeodomain-interacting protein kinase 2 (HIPK2) was required for the production of type I IFNs in response to RNA virus infection. HIPK2 deficiency markedly impaired IFN production in macrophages after vesicular stomatitis virus (VSV) infection, and HIPK2-deficient mice were more susceptible to lethal VSV disease than were wild-type mice. After VSV infection, HIPK2 was cleaved by active caspases, which released a hyperactive, N-terminal fragment that translocated to the nucleus and further augmented antiviral responses...
March 19, 2019: Science Signaling
https://read.qxmd.com/read/30774641/arabidopsis-jmjd5-jmj30-acts-independently-of-lux-arrhythmo-within-the-plant-circadian-clock-to-enable-temperature-compensation
#28
JOURNAL ARTICLE
Matthew A Jones, Kengo Morohashi, Erich Grotewold, Stacey L Harmer
The circadian system ensures that plants respond appropriately to environmental change by predicting regular transitions that occur during diel cycles. In order to be most useful, the circadian system needs to be compensated against daily and seasonal changes in temperature that would otherwise alter the pace of this biological oscillator. We demonstrate that an evening-phased protein, the putative histone demethylase JMJD5, contributes to temperature compensation. JMJD5 is co-expressed with components of the Evening Complex, an agglomeration of proteins including EARLY FLOWERING3 (ELF3), ELF4, and LUX ARRHYTHYMO (LUX), which also integrates temperature changes into the molecular clockwork...
2019: Frontiers in Plant Science
https://read.qxmd.com/read/30723133/noncanonical-inhibition-of-mtorc1-by-coxiella-burnetii-promotes-replication-within-a-phagolysosome-like-vacuole
#29
JOURNAL ARTICLE
Charles L Larson, Kelsi M Sandoz, Diane C Cockrell, Robert A Heinzen
The Q fever agent Coxiella burnetii is a Gram-negative bacterium that invades macrophages and replicates inside a specialized lysosomal vacuole. The pathogen employs a type 4B secretion system (T4BSS) to deliver effector proteins into the host cell that modify the Coxiella- containing vacuole (CCV) into a replication-permissive niche. Mature CCVs are massive degradative organelles that acquire lysosomal proteins. Inhibition of mammalian (or mechanistic) target of rapamycin complex 1 (mTORC1) kinase by nutrient deprivation promotes autophagy and lysosome fusion, as well as activation of the transcription factors TFE3 and TFEB (TFE3/B), which upregulates expression of lysosomal genes...
February 5, 2019: MBio
https://read.qxmd.com/read/30670603/response-of-the-circadian-clock-and-diel-starch-turnover-to-one-day-of-low-light-or-low-co2
#30
JOURNAL ARTICLE
Thiago Alexandre Moraes, Virginie Mengin, Maria Grazia Annunziata, Beatrice Encke, Nicole Krohn, Melanie Hoehne, Mark Stitt
Diel starch turnover responds rapidly to changes in the light regime. We investigated if these responses require changes in the temporal dynamics of the circadian clock. Arabidopsis thaliana was grown in a 12-h photoperiod for 19 days, shifted to three different reduced light levels or to low CO2 for one light period, and returned to growth conditions. The treatments produced widespread changes in clock transcript abundance. However, almost all of the changes were restricted to extreme treatments that led to carbon starvation and were small compared to the magnitude of the circadian oscillation...
January 22, 2019: Plant Physiology
https://read.qxmd.com/read/30606777/hos15-interacts-with-the-histone-deactetylase-hda9-and-the-evening-complex-to-epigenetically-regulate-the-floral-activator-gigantea
#31
JOURNAL ARTICLE
Hee Jin Park, Dongwon Baek, Joon-Yung Cha, Xueji Liao, Sang-Ho Kang, Robertson C McClung, Sang Yeol Lee, Dae Jin Yun, Woe-Yeon Kim
In plants, seasonal inputs such as photoperiod and temperature modulate the plant's internal genetic program to regulate the timing of the developmental transition from vegetative to reproductive growth. This regulation of the floral transition involves chromatin remodeling, including covalent modification of histones. Here, we report that HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 15 (HOS15), a WD40 repeat protein, associates with a histone deacetylase complex to repress transcription of the GIGANTEA (GI)-mediated photoperiodic flowering pathway in Arabidopsis thaliana...
January 3, 2019: Plant Cell
https://read.qxmd.com/read/30572255/in-vitro-analysis-of-immunomodulatory-effects-of-mesenchymal-stem-cell-and-tumor-cell-derived-exosomes-on-recall-antigen-specific-responses
#32
JOURNAL ARTICLE
Anwar Fathollahi, Seyed Mahmoud Hashemi, Mostafa Haji Molla Hoseini, Farshid Yeganeh
BACKGROUND: The aim of the present study was to evaluate in vitro effects of exosomes derived from mesenchymal stem cells (MSCs) or tumor cells on recall-antigen-specific immune responses. METHODS: The exosomes were isolated from the supernatant of the cultures of the adipose-derived MSCs, and 4T1 cell line. The splenocytes isolated from experimental autoimmune encephalomyelitis (EAE) mice were utilized to evaluate the effects of exosomes on recall-antigen-specific responses...
December 17, 2018: International Immunopharmacology
https://read.qxmd.com/read/30341009/bioselection-reveals-mir-99b-and-mir-485-as-enhancers-of-adenoviral-oncolysis-in-pancreatic-cancer
#33
JOURNAL ARTICLE
Maria Rovira-Rigau, Giulia Raimondi, Miguel Ángel Marín, Meritxell Gironella, Ramon Alemany, Cristina Fillat
Oncolytic viruses are designed for cancer treatment. Cell-virus interactions are key determinants for successful viral replication. Therefore, the extensive reprogramming of gene expression that occurs in tumor cells might create a hurdle for viral propagation. We used a replication-based approach of a microRNA (miRNA) adenoviral library encoding up to 243 human miRNAs as a bioselection strategy to identify miRNAs that facilitate adenoviral oncolytic activity in pancreatic ductal adenocarcinoma. We identify two miRNAs, miR-99b and miR-485, that function as enhancers of adenoviral oncolysis by improving the intra- and extracellular yield of mature virions...
September 27, 2018: Molecular Therapy
https://read.qxmd.com/read/30089112/mir-221-negatively-regulates-innate-anti-viral-response
#34
JOURNAL ARTICLE
Hongqiang Du, Shuang Cui, Yunfei Li, Guang Yang, Peiyan Wang, Erol Fikrig, Fuping You
The innate immune system plays a critical role in the initial antiviral response. However, the timing and duration of these responses must be tightly regulated during infection to ensure appropriate immune cell activation and anti-viral defenses. Here we demonstrate that during antiviral response, a negative regulator miR-221 was also induced in an ELF4-dependent manner. We further show that ELF4 promotes miR-221 expression through direct binding to its promoter. Overexpression and knockdown assay show that miR-221 can negatively regulate IFNβ production in time of virus infection...
2018: PloS One
https://read.qxmd.com/read/30065116/circadian-control-of-ore1-by-prr9-positively-regulates-leaf-senescence-in-arabidopsis
#35
JOURNAL ARTICLE
Hyunmin Kim, Hyo Jung Kim, Quy Thi Vu, Sukjoon Jung, C Robertson McClung, Sunghyun Hong, Hong Gil Nam
The circadian clock coordinates the daily cyclic rhythm of numerous biological processes by regulating a large portion of the transcriptome. In animals, the circadian clock is involved in aging and senescence, and circadian disruption by mutations in clock genes frequently accelerates aging. Conversely, aging alters circadian rhythmicity, which causes age-associated physiological alterations. However, interactions between the circadian clock and aging have been rarely studied in plants. Here, we investigated potential roles for the circadian clock in the regulation of leaf senescence in plants...
August 14, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/29991314/whole-genome-mrna-gene-expression-differs-between-patients-with-and-without-delirium
#36
JOURNAL ARTICLE
Katrina Kalantar, Sara C LaHue, Joseph L DeRisi, Hannah A Sample, Caitlin A Contag, Scott A Josephson, Michael R Wilson, Vanja C Douglas
OBJECTIVE: To identify differences in gene expression between patients with in-hospital delirium from a known etiology (urinary tract infection [UTI]) and patients with delirium from an unknown etiology, as well as from nondelirious patients. METHODS: Thirty patients with delirium (8 with UTI) and 21 nondelirious patients (11 with UTI) were included in this prospective case-control study. Transcriptomic profiles from messenger RNA sequencing of peripheral blood were analyzed for gene expression and disease-specific pathway enrichment patterns, correcting for systemic inflammatory response syndrome...
July 2018: Journal of Geriatric Psychiatry and Neurology
https://read.qxmd.com/read/29565554/a-complementary-chemical-and-genomic-screening-approach-for-druggable-targets-in-the-nrf2-pathway-and-small-molecule-inhibitors-to-overcome-cancer-cell-drug-resistance
#37
JOURNAL ARTICLE
James H Matthews, Xiao Liang, Valerie J Paul, Hendrik Luesch
Resistance to chemotherapy is a major obstacle in the treatment of a wide array of different types of cancer. Chemotherapeutic drug resistance is achieved by cancer cells by a variety of different mechanisms, which can be either compound specific or general. An emerging mechanism for nonspecific chemotherapeutic drug resistance relies on hyperactivity of the transcription factor Nrf2. Normally Nrf2 levels are tightly regulated by the ubiquitin-proteasome system; however, mutations in genes responsible for this regulation are common in many cancer types, resulting in increased expression of Nrf2, activation of its downstream target genes, and resistance to a variety of chemotherapeutic agents...
May 18, 2018: ACS Chemical Biology
https://read.qxmd.com/read/29535786/association-of-glioblastoma-multiforme-stem-cell-characteristics-differentiation-and-microglia-marker-genes-with-patient-survival
#38
JOURNAL ARTICLE
Sandra Bien-Möller, Ellen Balz, Susann Herzog, Laura Plantera, Silke Vogelgesang, Kerstin Weitmann, Carolin Seifert, Matthias A Fink, Sascha Marx, Angela Bialke, Chitra Venugopal, Sheila K Singh, Wolfgang Hoffmann, Bernhard H Rauch, Henry W S Schroeder
Patients with glioblastoma multiforme (GBM) are at high risk to develop a relapse despite multimodal therapy. Assumedly, glioma stem cells (GSCs) are responsible for treatment resistance of GBM. Identification of specific GSC markers may help to develop targeted therapies. Here, we performed expression analyses of stem cell (ABCG2, CD44, CD95, CD133, ELF4, Nanog, and Nestin) as well as differentiation and microglia markers (GFAP, Iba1, and Sparc) in GBM compared to nonmalignant brain. Furthermore, the role of these proteins for patient survival and their expression in LN18 stem-like neurospheres was analyzed...
2018: Stem Cells International
https://read.qxmd.com/read/29124042/e-fischeriana-root-compound-dpo-activates-antiviral-innate-immunity
#39
JOURNAL ARTICLE
Jingxuan Chen, Hongqiang Du, Shuang Cui, Tong Liu, Guang Yang, Huaping Sun, Weiwei Tao, Baoping Jiang, Li Yu, Fuping You
E. fischeriana has long been used as a traditional Chinese medicine. Recent studies reported that some compounds of E. fischeriana exhibited antimicrobial and immune enhance activity. Innate immune system is essential for the immune surveillance of inner and outer threats, initial host defense responses and immune modulation. The role of natural drug compounds, including E. fischeriana, in innate immune regulation is largely unknown. Here we demonstrated that E. fischeriana compound Dpo is involved in antiviral signaling...
2017: Frontiers in Cellular and Infection Microbiology
https://read.qxmd.com/read/29032592/arabidopsis-early-flowering3-increases-salt-tolerance-by-suppressing-salt-stress-response-pathways
#40
JOURNAL ARTICLE
Yasuhito Sakuraba, Selin Bülbül, Weilan Piao, Giltsu Choi, Nam-Chon Paek
Arabidopsis EARLY FLOWERING3 (ELF3) functions in modulating light input to the circadian clock, as a component of ELF3-ELF4-LUX ARRHYTHMO (LUX) evening complex. However, the role of ELF3 in stress responses remains largely unknown. In this study, we show that ELF3 enhances plants' resilience to salt stress: ELF3-overexpressing (ELF3-OX) plants are salt-tolerant, while elf3 mutants are more sensitive to salt stress. The expressions of many salt stress- and senescence-associated genes are altered in elf3-1 and ELF3-OX plants compared with wild-type...
December 2017: Plant Journal
keyword
keyword
88994
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.