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https://www.readbyqxmd.com/read/28646648/natural-history-of-a-viral-cohesive-end-site-cosn-of-the-%C3%AE-like-phages
#1
Nicole Vahanian, Choon Seok Oh, Jean Sippy, Michael Feiss
The base pairs of cosN, the site where the 12 base-long cohesive ends are generated in λ-like phages, show partial-two fold rotational symmetry. In a bioinformatic survey, we found that the cosN changes in 12 natural cosN variants are restricted to bp 6-to-12 of the cohesive end sequence. In contrast, bp 1-5 of the cohesive end sequence are strictly conserved (13/13), as are the two bp flanking the left nicking site (bp -2 and -1). The bp flanking the right nick site (bp 13 and 14) are conserved in 12 of 13 variants...
June 21, 2017: Virology
https://www.readbyqxmd.com/read/28646563/cohesive-network-reconfiguration-accompanies-extended-training
#2
Qawi K Telesford, Arian Ashourvan, Nicholas F Wymbs, Scott T Grafton, Jean M Vettel, Danielle S Bassett
Human behavior is supported by flexible neurophysiological processes that enable the fine-scale manipulation of information across distributed neural circuits. Yet, approaches for understanding the dynamics of these circuit interactions have been limited. One promising avenue for quantifying and describing these dynamics lies in multilayer network models. Here, networks are composed of nodes (which represent brain regions) and time-dependent edges (which represent statistical similarities in activity time series)...
June 24, 2017: Human Brain Mapping
https://www.readbyqxmd.com/read/28642208/assessing-the-efficacy-of-imagery-enhanced-cognitive-behavioral-group-therapy-for-social-anxiety-disorder-study-protocol-for-a-randomized-controlled-trial
#3
Peter M McEvoy, Michelle L Moulds, Jessica R Grisham, Emily A Holmes, David A Moscovitch, Delia Hendrie, Lisa M Saulsman, Ottmar V Lipp, Robert T Kane, Ronald M Rapee, Matthew P Hyett, David M Erceg-Hurn
Cognitive behavior group therapy (CBGT) is effective for social anxiety disorder (SAD), but a substantial proportion of patients do not typically achieve normative functioning. Cognitive behavioral models of SAD emphasize negative self-imagery as an important maintaining factor, and evidence suggests that imagery is a powerful cognitive mode for facilitating affective change. This study will compare two group CBGT interventions, one that predominantly uses verbally-based strategies (VB-CBGT) and another that predominantly uses imagery-enhanced strategies (IE-CBGT), in terms of (a) efficacy, (b) mechanisms of change, and (c) cost-effectiveness...
June 19, 2017: Contemporary Clinical Trials
https://www.readbyqxmd.com/read/28641585/cancer-bereaved-siblings-positive-and-negative-memories-and-experiences-of-illness-and-death-a-nationwide-follow-up
#4
Mary-Elizabeth Bradley Eilertsen, Malin Lövgren, Alexandra Eilegård Wallin, Ulrika Kreicbergs
OBJECTIVE: Our aim was to explore bereaved siblings' positive and negative memories and experiences of their brother's or sister's illness and death. METHOD: In our nationwide Swedish study, 174 of 240 (73%) bereaved siblings participated, and 70% responded to two open-ended statements, which focused on siblings' positive and negative memories and experiences of illness and death. The data were analyzed using systematic text condensation. RESULTS: The bereaved siblings' responses were categorized into four different themes: (1) endurance versus vulnerability, (2) family cohesion versus family conflicts, (3) growth versus stagnation, and (4) professional support versus lack of professional support...
June 23, 2017: Palliative & Supportive Care
https://www.readbyqxmd.com/read/28640685/understanding-and-improving-arterial-roads-to-support-public-health-and-transportation-goals
#5
Carolyn McAndrews, Keshia M Pollack, David Berrigan, Andrew L Dannenberg, Ed J Christopher
Arterials are types of roads designed to carry high volumes of motorized traffic. They are an integral part of transportation systems worldwide and exposure to them is ubiquitous, especially in urban areas. Arterials provide access to diverse commercial and cultural resources, which can positively influence community health by supporting social cohesion as well as economic and cultural opportunities. They can negatively influence health via safety issues, noise, air pollution, and lack of economic development...
June 22, 2017: American Journal of Public Health
https://www.readbyqxmd.com/read/28639483/assessing-narcissism-using-rorschach-based-imagery-and-behavior-validated-by-clinician-reports-studies-with-adult-patients-and-nonpatients
#6
Emanuela S Gritti, David P Marino, Margherita Lang, Gregory J Meyer
We evaluate 11 Rorschach variables with potential for assessing grandiosity and narcissism. Seven of these variables were drawn from previous literature: Omnipotence, Idealization, Reflection, Personal Knowledge Justification, Exhibitionism, Magic, and Elevated Mood States; four were developed for this research: Expanded Personal Reference, Narcissistic Devaluation, Narcissistic Deflation, and Narcissistic Denial. Using Rorschach protocols from American normative adults and Italian adult outpatients, the dimensional structure of these variables was evaluated by principal components analysis, and validity was tested by correlations with clinician ratings of narcissism on two scales from the Shedler-Westen Assessment Procedure-200 that were made after at least five sessions with the primary clinician...
June 1, 2017: Assessment
https://www.readbyqxmd.com/read/28639326/bone-marrow-hematons-an-access-point-to-the-human-hematopoietic-niche
#7
Alexandre Janel, Juliette Berger, Céline Bourgne, Richard Lemal, Nathalie Boiret-Dupré, Frédérique Dubois-Galopin, Pierre Déchelotte, Charlotte Bothorel, Eric Hermet, Sara Chabi, Jacques-Olivier Bay, Céline Lambert, Bruno Pereira, Françoise Pflumio, Rima Haddad, Marc G Berger
To understand the complex interactions between hematopoietic stem cells and the bone marrow niche, a human experimental model is needed. Our hypothesis is that hematons are an appropriate ex vivo model of human bone marrow. We analyzed the hierarchical hematopoietic cell content and the tissue organization of single hematons from healthy donors. Most (>90%) hematons contained precursors of all cell lineages, myeloid progenitors, and LTC-ICs without preferential commitment. Approximately half of the hematons could generate significant levels of lympho-myeloid hematopoiesis after transplantation in an NSG mouse model, despite the low absolute numbers of transplanted CD34(+) cells...
June 22, 2017: American Journal of Hematology
https://www.readbyqxmd.com/read/28639225/the-development-and-implementation-of-a-patient-continuity-conference-in-a-psychiatry-residency-program
#8
Claire Garber, Marianne Bernadino, Joshua Tomaskek, Katharine J Nelson
OBJECTIVE: A resident-led patient continuity case conference was initiated with the goals of improving communication among providers and increasing cohesion among residents. METHODS: A monthly case conference focusing on patient continuity of care was held over the course of the academic year. Residents were surveyed for feedback about the role of the conference in both improving their competency in navigating transitions of care and building cohesion among residents...
June 21, 2017: Academic Psychiatry
https://www.readbyqxmd.com/read/28638569/quality-improvement-for-hiv-screening-in-a-primary-care-resident-clinic
#9
Tracey Doering, Sagal Mohamed, Laura Hollar, Zachary Pugh
Primary care physicians are responsible for the initiation of the preventive health screening tests and examinations for their patients. Resident physicians may be uncomfortable obtaining a sexual history from their patient. A performance improvement project was carried out in the resident clinic to see if we could improve HIV screening of our patients. With a cohesive effort between the faculty preceptors and residents, we were able to improve screening rates for HIV and improve some aspects, but not all, of sexual history taking for our population of patients...
March 2017: Journal of Community Hospital Internal Medicine Perspectives
https://www.readbyqxmd.com/read/28637284/descriptive-texture-analyses-of-cooked-patties-made-of-chicken-breast-with-the-woody-breast-condition1
#10
G Sanchez Brambila, Debolina Chatterjee, B Bowker, H Zhuang
The woody breast (WB) condition negatively influences the texture characteristics and quality of intact broiler breast fillets (Pectoralis major). But the impact of WB on ground meat is unknown. The objective of this study was to evaluate the effects of WB on the texture and cook loss of ground meat made of broiler breast fillets. Broiler breasts (deboned 3 h postmortem) were collected on 3 separate trial d from the commercial deboning line and classified into normal and severe WB fillet categories. Individual fillets were either ground and formed into patties or left intact prior to being stored for 7 d at -20°C...
June 16, 2017: Poultry Science
https://www.readbyqxmd.com/read/28636013/new-insights-into-the-solubility-of-graphene-oxide-in-water-and-alcohols
#11
Vadim V Neklyudov, Nail R Khafizov, Igor A Sedov, Ayrat M Dimiev
One of the main advantages of graphene oxide (GO) over its non-oxidized counterpart is its ability to form stable solutions in water and some organic solvents. At the same time, the nature of GO solutions is not completely understood; the existing data are scarce and controversial. Here, we demonstrate that the solubility of GO, and the stability of the as-formed solutions depend not just on the solute and solvent cohesion parameters, as commonly believed, but mostly on the chemical interactions at the GO/solvent interface...
June 21, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28635480/leadership-styles-in-nursing
#12
Vicki Cope, Melanie Murray
Nurses are often asked to think about leadership, particularly in times of rapid change in healthcare, and where questions have been raised about whether leaders and managers have adequate insight into the requirements of care. This article discusses several leadership styles relevant to contemporary healthcare and nursing practice. Nurses who are aware of leadership styles may find this knowledge useful in maintaining a cohesive working environment. Leadership knowledge and skills can be improved through training, where, rather than having to undertake formal leadership roles without adequate preparation, nurses are able to learn, nurture, model and develop effective leadership behaviours, ultimately improving nursing staff retention and enhancing the delivery of safe and effective care...
June 21, 2017: Nursing Standard
https://www.readbyqxmd.com/read/28633909/resolving-in-vivo-gene-expression-during-collective-cell-migration-using-an-integrated-rnascope-immunohistochemistry-and-tissue-clearing-method
#13
Jason A Morrison, Mary Cathleen McKinney, Paul M Kulesa
During collective cell migration individual cells display diverse behaviors that complicate our understanding of group cell decisions of direction and cohesion. In vivo gene and protein expression analyses would shed light on the underlying molecular choreography. However, this information has been limited due to difficulties to integrate single cell detection methods and the simultaneous readout of signals deep within the embryo. Here, we optimize and integrate multiplex fluorescence in situ hybridization by RNAscope, immunohistochemistry, and tissue clearing to visualize transcript and protein localization within single cells deep within intact chick embryos...
June 17, 2017: Mechanisms of Development
https://www.readbyqxmd.com/read/28633523/the-effect-of-pseudomonas-fluorescens-on-buried-steel-pipeline-corrosion
#14
Amy J Spark, Ivan S Cole, Adam S Best, David W Law, Liam P Ward
Buried steel infrastructure can be a source of iron ions for bacterial species, leading to microbiologically influence corrosion (MIC). Localised corrosion of pipelines due to MIC is one of the key failure mechanisms of buried steel pipelines. In order to better understand the mechanisms of localised corrosion in soil, semi-solid agar has been developed as an analogue for soil. Here, Pseudomonas fluorescens has been introduced to the system to understand how bacteria interact with steel. Through electrochemical testing including open circuit potentials, potentiodynamic scans, anodic potential holds and electrochemical impedance spectroscopy it has been shown that P...
June 21, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28630293/a-null-model-for-microbial-diversification
#15
Timothy J Straub, Olga Zhaxybayeva
Whether prokaryotes (Bacteria and Archaea) are naturally organized into phenotypically and genetically cohesive units comparable to animal or plant species remains contested, frustrating attempts to estimate how many such units there might be, or to identify the ecological roles they play. Analyses of gene sequences in various closely related prokaryotic groups reveal that sequence diversity is typically organized into distinct clusters, and processes such as periodic selection and extensive recombination are understood to be drivers of cluster formation ("speciation")...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28628076/promoting-simulation-globally-networking-with-nursing-colleagues-across-five-continents
#16
Celeste M Alfes, Elizabeth A Madigan
Simulation education is gaining momentum internationally and may provide the opportunity to enhance clinical education while disseminating evidence-based practice standards for clinical simulation and learning. There is a need to develop a cohesive leadership group that fosters support, networking, and sharing of simulation resources globally. The Frances Payne Bolton School of Nursing at Case Western Reserve University has had the unique opportunity to establish academic exchange programs with schools of nursing across five continents...
July 2017: Nursing Education Perspectives
https://www.readbyqxmd.com/read/28627717/direction-dependent-contraction-forces-on-cell-boundaries-induce-collective-migration-of-epithelial-cells-within-their-sheet
#17
REVIEW
Katsuhiko Sato
During early embryonic development, epithelial cells form a monolayer sheet and migrate in a definite direction. This phenomenon, called epithelial cell migration, is an important topic in developmental biology. A characteristic feature of this process is attachment to adjacent cells during migration, which is necessary for maintaining the integrity of the sheet. However, it is unclear how these cohesive cells migrate without breaking their attachments. A mechanism for this phenomenon was recently proposed, in which direction-dependent contraction forces acting on cell boundaries induce unidirectional epithelial migration...
June 19, 2017: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/28627627/stromal-antigen-2-functions-as-a-tumor-suppressor-in-bladder-cancer-cells
#18
Han Wang, Jianhua Zhong, Chenglong Wu, Yang Liu, Jinghua Zhang, Xiaowen Zou, Yan Chen, Jinfeng Su, Gang Yang, Yuantang Zhong, Aifa Tang
Stromal antigen 2 (STAG2) is a subunit of the cohesion complex that plays an important role in the normal segregation of sister chromatids during mitosis or meiosis. However, the effect of STAG2 on the bladder cancer cell proliferation, migration, and invasion has not yet been fully clarified. In this study, we aimed to characterize STAG2 expression and functional significance in BC and adjacent normal tissue. Notably, STAG2 expression was markedly lower in BC cells and tumor tissues than their normal counterparts at the gene and protein levels...
June 12, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28626133/simple-synthesis-hydrogenated-castor-oil-fatty-amide-wax-and-its-coating-characterization
#19
Xiuzhu Yu, Ning Wang, Rui Zhang, Zhong Zhao
A simple method for incorporating amine groups in hydrogenated castor oil (HCO) to produce wax for beeswax or carnauba wax substitution in packaging and coating was developed. From the conversion rate of the products, HCO was reacted with ethanolamine at 150°C for 5 h, and the molar ratio of HCO and ethanolamine was 1:4. The hardness of the final product was seven times higher than that of beeswax, the cohesiveness of the final product was 1.3 times higher than that of beeswax and approximately one half of that of carnauba wax, and the melting point of the final product is 98°C...
June 13, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28625229/mass-gathering-medical-care-in-electronic-dance-music-festivals
#20
Kathleen M FitzGibbon, Jose V Nable, Benjamin Ayd, Benjamin J Lawner, Angela C Comer, Richard Lichenstein, Matthew J Levy, Kevin G Seaman, Ian Bussey
Introduction Electronic dance music (EDM) festivals represent a unique subset of mass-gathering events with limited guidance through literature or legislation to guide mass-gathering medical care at these events. Hypothesis/Problem Electronic dance music festivals pose unique challenges with increased patient encounters and heightened patient acuity under-estimated by current validated casualty predication models. METHODS: This was a retrospective review of three separate EDM festivals with analysis of patient encounters and patient transport rates...
June 19, 2017: Prehospital and Disaster Medicine
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